Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| the monomeric gtpase rab35 regulates phagocytic cup formation and phagosomal maturation in entamoeba histolytica. | one of the hallmarks of amoebic colitis is the detection of entamoeba histolytica (eh) trophozoites with ingested erythrocytes. therefore, erythrophagocytosis is traditionally considered as one of the most important criteria to identify the pathogenic behavior of the amoebic trophozoites. phagocytosis is an essential process for the proliferation and virulence of this parasite. phagocytic cargo, upon internalization, follows a defined trafficking route to amoebic lysosomal degradation machinery. ... | 2017 | 28126902 |
| investigating the mechanism of adp-forming acetyl-coa synthetase from the protozoan parasite entamoeba histolytica. | adp-forming acetyl-coa synthetase (acd) catalyzes the interconversion of acetyl-coa and acetate. the related succinyl-coa synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for pyrococcus acd. characterization of enzyme variants of entamoeba acd in which the two proposed phosphorylated his residues were individually altered revealed that only his252 is essential for enzymatic activity. anal ... | 2017 | 28129670 |
| sine polymorphism reveals distinct strains of entamoeba histolytica from north india. | entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality in the developing world. more tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. virulence pattern of e. histolytica could be linked with the genotype of a strain. several loci showing insertion polymorphism of retrotransposable short interspersed nuclear elements ehsine1 and ehsine2 have been reported among laboratory strains of e. histolytica. the present study was ... | 2017 | 28131659 |
| prevalence and seasonal variation of human intestinal parasites in patients attending hospital with abdominal symptoms in northern jordan. | this study was carried out to determine the prevalence of intestinal parasites and their seasonal variation in northern jordan. a total of 21 906 stool samples were collected over a period of 4 years (2009- 2013) from 5 government hospitals in 3 cities. samples were processed and examined microscopically and by concentration methods. parasitic infection was found in 9611 samples (44%). giardia lamblia was the most prevalent parasite (41%) followed by entamoeba histolytica (31%) and ent. coli (13 ... | 2017 | 28134428 |
| first molecular epidemiology of entamoeba histolytica, e. dispar and e. moshkovskii infections in yemen: different species-specific associated risk factors. | to investigate the molecular epidemiology of entamoeba histolytica, e. dispar and e. moshkovskii infections among rural communities in yemen. | 2017 | 28151567 |
| epidemiology of soil-transmitted helminth and intestinal protozoan infections in preschool-aged children in the amhara region of ethiopia. | intestinal parasites are important contributors to global morbidity and mortality and are the second most common cause of outpatient morbidity in ethiopia. this cross-sectional survey describes the prevalence of soil-transmitted helminths and intestinal protozoa in preschool children 0-5 years of age in seven communities in the amhara region of ethiopia, and investigates associations between infection, household water and sanitation characteristics, and child growth. stool samples were collected ... | 2017 | 28167597 |
| [ambiguous hepatic mass and fever after stay in turkey]. | we report the case of a 76-year old female patient with a hepatic mass after staying in eastern turkey. there were no indices for malignancy or an infection with echinococcus or entamoeba histolytica. finally we diagnosed a fascioliasis (liver fluke) and cured the patient successfully. | 2017 | 28180910 |
| synthesis and biological evaluation of newer 1,3,4-oxadiazoles incorporated with benzothiazepine and benzodiazepine moieties. | a series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (staphylococcus aureus, staphylococcus pyogenes, escherichia coli, and pseudomonas aeruginosa) and fungi (candida albicans, aspergillus niger, and aspergillus clavatus) and protozoa (entamoeba histolytica, giardia lamblia, trypanosoma cruzi and leishmania mexicana). few of the selected compounds were tested for their ant ... | 2017 | 28182579 |
| entamoeba histolytica-encoded homolog of macrophage migration inhibitory factor contributes to mucosal inflammation during amebic colitis. | 2017 | 28186296 | |
| antiprotozoal constituents from annona cherimola miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery. | annona cherimola miller (annonaceae) is a medicinal plant frequently recommended in mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery. | 2017 | 28216899 |
| antiprotozoal, antimycobacterial, and anti-inflammatory evaluation of cnidoscolus chayamansa (mc vaugh) extract and the isolated compounds. | cnidoscolus chayamansa is a medicinal and edible plant known as chaya, is commonly used as an anti-inflammatory, antiprotozoal, antibacterial agent and as a remedy for respiratory illness, gastrointestinal disorders, and vaginal infections related with the inflammation process. in this paper, we describe the plant's phytochemical analysis and biological activities (antimycobacterial, antibacterial, antiprotozoal, and anti-inflammatory properties) of the chcl3:meoh (1:1) leaves extract and isolat ... | 2017 | 28222400 |
| prevalence of intestinal parasites in referred individuals to the medical centers of tonekabon city, mazandaran province. | the aim of the present study was to determine the prevalence of intestinal parasites and their relation with socio-demographic data in referred individuals to the medical centers in tonekabon, mazandaran province, 2015. | 2016 | 28224032 |
| occurrence of giardia, cryptosporidium, and entamoeba in wild rhesus macaques (macaca mulatta) living in urban and semi-rural north-west india. | giardia duodenalis, cryptosporidium spp., and entamoeba spp. are intestinal protozoa capable of infecting a range of host species, and are important causes of human morbidity and mortality. understanding their epidemiology is important, both for public health and for the health of the animals they infect. this study investigated the occurrence of these protozoans in rhesus macaques (macaca mulatta) in india, with the aim of providing preliminary information on the potential for transmission of t ... | 2017 | 28229045 |
| role of eosinophils and tumor necrosis factor alpha in interleukin-25-mediated protection from amebic colitis. | the parasite entamoeba histolytica is a cause of diarrhea in infants in low-income countries. previously, it was shown that tumor necrosis factor alpha (tnf-α) production was associated with increased risk of e. histolytica diarrhea in children. interleukin-25 (il-25) is a cytokine that is produced by intestinal epithelial cells that has a role in maintenance of gut barrier function and inhibition of tnf-α production. il-25 expression was decreased in humans and in the mouse model of amebic coli ... | 2017 | 28246365 |
| geospatial distribution of intestinal parasitic infections in rio de janeiro (brazil) and its association with social determinants. | intestinal parasitic infections remain among the most common infectious diseases worldwide. this study aimed to estimate their prevalence and provide a detailed analysis of geographical distribution of intestinal parasites in the metropolitan region of rio de janeiro, considering demographic, socio-economic, and epidemiological contextual factors. | 2017 | 28273080 |
| behavior of dna-lacking mitochondria in entamoeba histolytica revealed by organelle transplant. | the anaerobic protozoan parasite entamoeba histolytica has mitosomes that are mitochondria lacking some canonical functions and organelle dna. mitosomes play an important role in the life cycle of the parasite. the distribution of proteins in mitosomes is not uniform, and how mitosomes are maintained and retained is unknown. to answer these questions, we developed a transplant method for mitosomes with hemagglutinin-tagged protein into recipient cells containing mitosomes with myc-tagged protein ... | 2017 | 28287148 |
| the tudor staphylococcal nuclease protein of entamoeba histolytica participates in transcription regulation and stress response. | entamoeba histolytica is the protozoa parasite responsible of human amoebiasis, disease that causes from 40,000 to 100,000 deaths annually worldwide. however, few are known about the expression regulation of molecules involved in its pathogenicity. transcription of some virulence-related genes is positively controlled by the cis-regulatory element named ure1. previously we identified the transcription factor that binds to ure1, which displayed a nuclear and cytoplasmic localization. this protein ... | 2017 | 28293543 |
| association between enteric protozoan parasites and gastrointestinal illness among hiv- and tuberculosis-infected individuals in the chowke district, southern mozambique. | human immune deficiency virus (hiv) and tuberculosis (tb) infections remain major public health issues globally, particularly in sub-saharan africa. impairment of both cell-mediated and humoral immunity by hiv and/or tb infections may limit the host's defences against other pathogens, including the diarrheagenic protozoan cryptosporidium spp., giardia intestinalis, and entamoeba histolytica. during september-december 2015 a cross-sectional study was conducted to assess the prevalence and molecul ... | 2017 | 28302528 |
| structural and functional studies of a noncanonical dicer from entamoeba histolytica. | rnaseiii proteins are dsrna-specific endonucleases involved in many important biological processes, such as small rna processing and maturation in eukaryotes. various small rnas have been identified in a protozoan parasite entamoeba histolytica. ehrnaseiii is the only rnaseiii endonuclease domain (riiid)-containing protein in e. histolytica. here, we present three crystal structures that reveal several unique structural features of ehrnaseiii, especially the interactions between the two helixes ... | 2017 | 28317870 |
| development and initial evaluation of a lateral flow dipstick test for antigen detection of entamoeba histolytica in stool sample. | entamoeba histolytica infection remains a public health concern in developing countries. early diagnosis of amoebiasis can avoid disease complications, thus this study was aimed at developing a test that can rapidly detect the parasite antigens in stool samples. rabbits were individually immunized with recombinant pyruvate phosphate dikinase (rppdk) and e. histolytica excretory-secretory antigens to produce polyclonal antibodies. a rapid dipstick test was produced using anti-rppdk pab lined on t ... | 2017 | 28335696 |
| spontaneous resolution of a hepatogastric fistula. | 2017 | 28345399 | |
| amoeboma in a saudi resident: a case report. | amoebiasis is the third most frequent cause of mortality after malaria and schistosomiasis. in developed countries, amebiasis is also seen in migrants who have travelled to endemic areas. the factors responsible for its progression from intestinal amebiasis to an amebic liver abscess are not fully understood. | 2016 | 28348756 |
| prevalence of entamoeba histolytica, giardia lamblia, and cryptosporidium spp. in libya: 2000-2015. | introduction the intestinal protozoa entamoeba histolytica, giardia lamblia, and cryptosporidium spp. are the causative agents of giardiasis, amebiasis, and cryptosporidiosis, respectively. adequate knowledge of the geographical distribution of parasites and the demographic variables that influence their prevalence is important for effective control of infection in at-risk populations. methods the data were obtained by an english language literature search of medline and pubmed for papers using ... | 2016 | 28349839 |
| entamoeba histolytica - tumor necrosis factor: a fatal attraction. | 2015 | 28357296 | |
| in entamoeba histolytica, a bspa family protein is required for chemotaxis toward tumour necrosis factor. | entamoeba histolytica cell migration is essential for the development of human amoebiasis (an infectious disease characterized by tissue invasion and destruction). the tissue inflammation associated with tumour necrosis factor (tnf) secretion by host cells is a well-documented feature of amoebiasis. tumour necrosis factor is a chemoattractant for e. histolytica, and the parasite may have a tnf receptor at its cell surface. | 2015 | 28357299 |
| stem loop-mediated isothermal amplification test: comparative analysis with classical lamp and pcr in detection of entamoeba histolytica in kenya. | entamoeba histolytica, the causative agent for amoebiasis is a considerable burden to population in the developing countries where it accounts for over 50 million infections. the tools for detection of amoebiasis are inadequate and diagnosis relies on microscopy which means a significant percent of cases remain undiagnosed. moreover, tests formats that can be rapidly applied in rural endemic areas are not available. | 2017 | 28359328 |
| the effects of phosphanegold(i) thiolates on the biological properties of acanthamoeba castellanii belonging to the t4 genotype. | gold compounds have shown promise in the treatment of non-communicable diseases such as rheumatoid arthritis and cancer, and are considered of value as anti-microbial agents against gram-negative and gram-positive bacteria, and have anti-parasitic properties against schistosoma mansoni, trypanosoma brucei, plasmodium falciparum, leishmania infantinum, giardia lamblia, and entamoeba histolytica. they are known to affect enzymatic activities that are required for the cellular respiration processes ... | 2017 | 28366172 |
| development of a thermostabilised triplex lamp assay with dry-reagent four target lateral flow dipstick for detection of entamoeba histolytica and non-pathogenic entamoeba spp. | this study highlighted the development of a four target nitrocellulose-based nucleic acid lateral flow immunoassay biosensor in a dry-reagent strip format for interpretation of double-labelled double-stranded amplicons from thermostabilised triplex loop-mediated isothermal amplification assay. the dna biosensor contained two test lines which captured biotin and texas red labelled amplicons; a lamp internal amplification control line that captured digoxigenin labelled amplicon; and a chromatograp ... | 2017 | 28372729 |
| clinical study on the efficacy of amoebex (coded herbal drug) compared with metronidazole for the treatment of amoebic dysentery. | amoebiasisis an infectious disease, which originated with the single-celled parasitic protozoan entamoeba histolytica. the parasitic amoeba infects the liver and intestine and may cause mild diarrhea and serious dysentery with bloody and mucoid stool. a study was conducted to evaluate the efficacy of amoebex (400mg), a herbal formulation for the treatment of amoebiasis infections as compared to that of metronidazole (400mg). the therapeutic evaluations of these medicines were carried out on 184 ... | 2016 | 28375117 |
| corrigendum: n-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of entamoeba histolytica to nitrosative stress. | 2017 | 28378758 | |
| detection and differentiation of entamoeba histolytica and entamoeba dispar in clinical samples through pcr-denaturing gradient gel electrophoresis. | amebiasis is one of the twenty major causes of disease in mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. in this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) to differentiate between entamoeba histolytica (pathogenic) and e. dispar (non-pathogenic). the target for the pcr amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivi ... | 2017 | 28380216 |
| employing binol-phosphoroselenoyl chloride for selective inositol phosphorylation and chemical synthesis of glycosyl inositol phospholipid from entamoeba histolytica. | here we describe the chemical synthesis of glycosyl inositol phospholipids from entamoeba histolytica. the key feature of this synthesis is a regioselective phosphorylation reaction that occurs via desymmetrization of a myo-inositol derivative with phosphoroselenoyl chloride. we also developed a new protecting group strategy that utilizes allyl and alloc groups to synthesize complex glycolipids bearing unsaturated lipids. these developments provided an efficient synthetic route for various compl ... | 2017 | 28390155 |
| multiplex pcr detection of cryptosporidium sp, giardia lamblia and entamoeba histolytica directly from dried stool samples from guinea-bissauan children with diarrhoea. | in developing countries, diarrhoea is the most common cause of death for children under five years of age, with giardia lamblia, cryptosporidium and entamoeba histolytica as the most frequent pathogenic parasites. traditional microscopy for stool parasites has poor sensitivity and specificity, while new molecular methods may provide more accurate diagnostics. in poor regions with sample storage hampered by uncertain electricity supply, research would benefit from a method capable of analysing dr ... | 2017 | 28446068 |
| entamoeba histolytica: host parasite interactions at the colonic epithelium. | entamoeba histolytica (eh) is the protozoan parasite responsible for intestinal amebiasis and interacts dynamically with the host intestinal epithelium during disease pathogenesis. a multifaceted pathogenesis profile accounts for why 90% of individuals infected with eh are largely asymptomatic. for 100 millions individuals that are infected each year, key interactions within the intestinal mucosa dictate disease susceptibility. the ability for eh to induce amebic colitis and disseminate into ext ... | 2017 | 28452682 |
| occurrence and removal efficiency of parasitic protozoa in swedish wastewater treatment plants. | giardia intestinalis, cryptosporidium spp., entamoeba histolytica and dientamoeba fragilis are parasitic protozoa and causative agents of gastroenteritis in humans. g. intestinalis and cryptosporidium spp. in particular are the most common protozoa associated with waterborne outbreaks in high-income countries. surveillance of protozoan prevalence in wastewater and evaluation of wastewater treatment removal efficiencies of protozoan pathogens is therefore imperative for assessment of human health ... | 2017 | 28458199 |
| detection of cryptosporidium in stool samples of immunocompromised patients. | cryptosporidium species is the most common opportunistic enteric parasite encountered in the immunocompromised patients. considering the need to diagnose them early relies mostly on rapid tests such as antigen detection by immunochromatographic test (ict), elisa, and microscopy. however, the sensitivity and specificity varies with different methods and different kits used. this study was conducted to determine the intestinal parasitic profile in immunocompromised patients and to assess the diagn ... | 2017 | 28459014 |
| proliferating cell nuclear antigen restores the enzymatic activity of a dna ligase i deficient in dna binding. | proliferating cell nuclear antigen (pcna) coordinates multienzymatic reactions by interacting with a variety of protein partners. family i dna ligases are multidomain proteins involved in sealing of dna nicks during okazaki fragment maturation and dna repair. the interaction of dna ligases with the interdomain connector loop (idcl) of pcna through its pcna-interacting peptide (pip box) is well studied but the role of the interacting surface between both proteins is not well characterized. in thi ... | 2017 | 28469979 |
| an anti-amoebic vaccine: generation of the recombinant antigen lc3 from entamoeba histolytica linked to mutated exotoxin a (peδiii) via the pichia pastoris system. | to generate an immunogenic chimeric protein containing the entamoeba histolytica lc3 fragment fused to the retrograde delivery domains of exotoxin a of pseudomonas aeruginosa and kdel3 for use as an effective vaccine. | 2017 | 28470625 |
| ehrho1 regulates plasma membrane blebbing through pi3 kinase in entamoeba histolytica. | the protozoan parasite entamoeba histolytica causes amoebiasis, a major public health problem in developing countries. motility of e. histolytica is important for its pathogenesis. blebbing is an essential process contributing to cellular motility in many systems. in mammalian cells, formation of plasma membrane blebs is regulated by rho-gtpases through its effectors, such as rho kinase, mdia1, and acto-myosin proteins. in this study, we have illuminated the role of ehrho1 in bleb formation and ... | 2017 | 28477431 |
| treatment of diarrhoea using traditional medicines: contemporary research in south africa and zimbabwe. | gastrointestinal disorders, diarrhoea in particular remain a major concern in south africa and zimbabwe resulting in high mortality rates when left untreated. this investigation was aimed at documenting herbal medicines used in the treatment of diarrhoea in south africa and zimbabwe. | 2016 | 28480353 |
| the e. histolytica genome structure and virulence. | the outcome of an entamoeba histolytica infection is variable and the contribution of genetic diversity within e. histolytica to human disease is not fully understood. the information provided by the whole genome sequence of the e. histolytica reference laboratory strain (hm-1:imss) and thirteen additional laboratory strains have been made publically available. in this review theories on the source of the unexpected level of structural diversity found in e. histolytica will be discussed. | 2016 | 28497014 |
| crystal structure of the entamoeba histolytica rna lariat debranching enzyme ehdbr1 reveals a catalytic zn(2+) /mn(2+) heterobinucleation. | the rna lariat debranching enzyme, dbr1, is a metallophosphoesterase that cleaves 2'-5' phosphodiester bonds within intronic lariats. previous reports have indicated that dbr1 enzymatic activity is supported by diverse metal ions including ni(2+) , mn(2+) , mg(2+) , fe(2+) , and zn(2+) . while in initial structures of the entamoeba histolytica dbr1 only one of the two catalytic metal-binding sites were observed to be occupied (with a mn(2+) ion), recent structures determined a zn(2+) /fe(2+) het ... | 2017 | 28504306 |
| structure of ca2+-binding protein-6 from entamoeba histolytica and its involvement in trophozoite proliferation regulation. | cell cycle of entamoeba histolytica, the etiological agent of amoebiasis, follows a novel pathway, which includes nuclear division without the nuclear membrane disassembly. we report a nuclear localized ca2+-binding protein from e. histolytica (abbreviated hereafter as ehcabp6), which is associated with microtubules. we determined the 3d solution nmr structure of ehcabp6, and identified one unusual, one canonical and two non-canonical cryptic ef-hand motifs. the cryptic ef-ii and ef-iv pair with ... | 2017 | 28505197 |
| parallel elisas using crude soluble antigen and excretory-secretory antigen for improved serodiagnosis of amoebic liver abscess. | crude soluble antigen (csa) produced from entamoeba histolytica trophozoite is conventionally used for serodiagnosis of invasive amoebiasis. however, high background seropositivities by csa-assay in endemic areas complicate the interpretation of positive result in clinical settings. instead, incorporating a second assay which indicates active or recent infection into the routine amoebic serology could possibly complement the limitations of csa-assay. hence, the present study aimed to evaluate th ... | 2017 | 28506795 |
| prevalence of parasitic contamination in salad vegetables collected from supermarkets and street vendors in amman and baqa’a - jordan. | one of the main ways in transmitting parasites to humans is through consuming contaminated raw vegetables. the aim of this study was to evaluate the prevalence of parasitological contamination (helminthes eggs, giardia and entamoeba histolytica cysts) of salad vegetables sold at supermarkets and street vendors in amman and baqa’a – jordan. a total of 133 samples of salad vegetables were collected and examined for the prevalence of parasites. it was found that 29% of the samples were contaminated ... | 2016 | 28517923 |
| antiprotozoal, antibacterial and antidiarrheal properties from the flowers of chiranthodendron pentadactylon and isolated flavonoids. | chiranthodendron pentadactylon larreat. (sterculiaceae) is a mexican plant used in traditional medicine for the treatment of heart disease symptoms and infectious diarrhea. | 2017 | 28539715 |
| antiprotozoal activities of tiliroside and other compounds from sphaeralcea angustifolia (cav.) g. don. | sphaeralcea angustifolia (malvaceae) is extensively used in mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery. | 2017 | 28539736 |
| measuring changes in transmission of neglected tropical diseases, malaria, and enteric pathogens from quantitative antibody levels. | serological antibody levels are a sensitive marker of pathogen exposure, and advances in multiplex assays have created enormous potential for large-scale, integrated infectious disease surveillance. most methods to analyze antibody measurements reduce quantitative antibody levels to seropositive and seronegative groups, but this can be difficult for many pathogens and may provide lower resolution information than quantitative levels. analysis methods have predominantly maintained a single diseas ... | 2017 | 28542223 |
| hookworm infection is associated with decreased cd4+ t cell counts in hiv-infected adult ugandans. | most studies evaluating epidemiologic relationships between helminths and hiv have been conducted in the pre-art era, and evidence of the impact of helminth infections on hiv disease progression remains conflicting. less is known about helminth infection and clinical outcomes in hiv-infected adults receiving antiretroviral therapy (art). we sampled hiv-infected adults for eight gastrointestinal parasites and correlated parasitic infection with demographic predictors, and clinical and immunologic ... | 2017 | 28542260 |
| high prevalence of enteroparasites in children from ribeirão preto, são paulo, brazil. | to determine the prevalence of intestinal parasites and possible associated factors. | 2017 | 28562805 |
| identification of s-nitrosylated (sno) proteins in entamoeba histolytica adapted to nitrosative stress: insights into the role of sno actin and in vitro virulence. | we have recently reported that entamoeba histolytica trophozoites can adapt to toxic levels of the nitric oxide (no) donor, s-nitrosoglutathione (gsno). even if the consequences of this adaptation on the modulation of gene expression in no-adapted trophozoites (nat) have been previously explored, insight on s-nitrosylated (sno) proteins in nat is missing. our study aims to fill this knowledge gap by performing a screening of sno proteins in nat. employing sno resin-assisted capture (rac), we ide ... | 2017 | 28589096 |
| synthesis, antiprotozoal activity, and chemoinformatic analysis of 2-(methylthio)-1h-benzimidazole-5-carboxamide derivatives: identification of new selective giardicidal and trichomonicidal compounds. | a series of twelve new 2-(methylthio)-1h-benzimidazole-5-carboxamide derivatives (1-12) were synthesized and their antiparasitic activity was tested in vitro against giardia intestinalis, trichomonas vaginalis and entamoeba histolytica. experimental evaluations showed ic50 values within the nanomolar range for all tested compounds, some showing higher activity than metronidazole and albendazole. a chemoinformatic study was used to compare the structure-activity relationship of the synthesized ca ... | 2017 | 28595066 |
| investigation of a food-borne outbreak of gastroenteritis in a school canteen revealed a variant of sapovirus genogroup v not detected by standard pcr, sollentuna, sweden, 2016. | a food-borne outbreak of gastroenteritis with more than 650 suspected cases occurred in april 2016 in sollentuna, sweden. it originated in a school kitchen serving a total of 2,700 meals daily. initial microbiological testing (for campylobacter, salmonella, shigella, yersinia, giardia, cryptosporidium, entamoeba histolytica, adeno-, astro-, noro-, rota- and sapovirus) of stool samples from 15 symptomatic cases was negative, despite a clinical presentation suggestive of calicivirus. analyses of t ... | 2017 | 28602163 |
| entamoeba histolytica infection in female sex workers: a matched case-control study in durango, mexico. | infection with entamoeba histolytica (e. histolytica) can be potentially transmitted by sexual contact. the seroepidemiology of e. histolytica in female sex workers has not been studied. the aim of the study was to determine whether e. histolytica is associated with the occupation of female sex work. in addition, the correlates of e. histolytica seroprevalence in female sex workers were also investigated. | 2017 | 28611864 |
| heavy subunit of cell surface gal/galnac lectin (hgl) undergoes degradation via endo-lysosomal compartments in entamoeba histolytica. | the human gut parasite entamoeba histolytica uses a multifunctional virulence factor, hgl, a cell surface transmembrane receptor subunit of gal/galnac lectin that contributes to adhesion, invasion, cytotoxicity and immune response in the host. at present, the physiologic importance of hgl receptor is mostly known for pathogenicity of e. histolytica. however, the molecular mechanisms of hgl trafficking events and their association with the intracellular membrane transport machinery are largely un ... | 2017 | 28613117 |
| intestinal parasitic infestation among paediatric diarrhoea patients attending hospitals in port blair, andaman and nicobar islands. | acute childhood diarrhoea remains one of the leading causes of childhood morbidity and mortality in developing countries. the present study was conducted to estimate the prevalence of various intestinal parasitic infestation among children aged 5 years and less suffering from acute diarrhoea disease attending hospitals in andaman islands. during the period from january 2012 to december 2013, a total of 675 acute diarrhoea patients were included in the study. among these samples, 476 (70.5, 95 % ... | 2017 | 28615884 |
| antiamoebic activity of petiveria alliacea leaves and their main component, isoarborinol. | petiveria alliacea l. (phytolacaceae) is a medicinal plant with a broad range of traditional therapeutic properties, including the treatment of dysentery and intestinal infections caused by protozoan parasites. however, its effects against entamoeba histolytica have not been reported yet. we investigated the antiamoebic activity present in the leaves of p. alliacea antiamoebic activity was evaluated in methanolic and aqueous extracts, as well as in the hexanic, methanolic, and etoac fractions. t ... | 2017 | 28621111 |
| enteric multiplex pcr panels: a new diagnostic tool for amoebic liver abscess? | invasive amoebiasis is a life-threatening infection requiring immediate detection and treatment. however, diagnosis is challenging because conventional methods such as light microscopy and serology are unreliable. molecular techniques are therefore considered the new diagnostic reference standard, but most of the developed assays are research tools and not widely available. recently commercial multiplex pcr panels have been introduced which permit the simultaneous detection of multiple enteric p ... | 2017 | 28626584 |
| multiplex pcr gastro-intestinal panels for the management of traveller's diarrhea : are they accurate and useful? | until recently, the search for enteropathogens causing travellers' diarrhea was based on stool culture (campylobacter spp., salmonella spp. and shigella spp.), direct microscopy with (cryptosporidium spp.) or without specific staining (giardia lamblia, entamoeba histolytica) or specific antigen detection (giardia lamblia, entamoeba histolytica). molecular analyses are progressively replacing traditional diagnostic methods but their clinical usefulness remains to be better defined. this article a ... | 2017 | 28627855 |
| profile of intestinal parasitosis among school-aged children in kiliba (eastern dr congo). | this study of feces sought to determine the prevalence of human intestinal parasites in the kiliba area of the eastern dr congo. feces of 602 school children aged from 9 to 20 years were collected from april to june 2014 and examined. direct analyses were supplemented with the kato and ritchie methods. the global prevalence of intestinal parasitosis in school-aged children in the kiliba area was 91.4 % [95 % ci: 87.6-94.3%]. the frequency of helminthiasis was high at 73.8 % (95 % ci: 68.4-78.6%) ... | 2017 | 28655684 |
| adherens junctions and desmosomes are damaged by entamoeba histolytica: participation of ehcpadh complex and ehcp112 protease. | entamoeba histolytica trophozoites adhere to epithelium at the cell-cell contact and perturb tight junctions disturbing the transepithelial electrical resistance. behind tight junctions are the adherens junctions (ajs) that reinforce them and the desmosomes (dsms) that maintain the epithelium integrity. the damage produced to ajs and dmss by this parasite is unknown. here, we studied the effect of the trophozoites, the ehcpadh complex, and the ehcp112 recombinant enzyme (rehcp112) on aj and dsm ... | 2017 | 28656597 |
| an integrated study of human and animal infectious disease in the lake victoria crescent small-holder crop-livestock production system, kenya. | the neglected zoonotic diseases (nzd) are an understudied group that are a major cause of illness throughout the developing world. in general, little is known about the prevalence and burden of nzds in affected communities, particularly in relation to other infectious diseases with which they are often co-endemic. we describe the design and descriptive epidemiological outputs from an integrated study of human and animal zoonotic and non-zoonotic disease in a rural farming community in western ke ... | 2017 | 28666412 |
| in-silico prediction and modeling of the entamoeba histolytica proteins: serine-rich entamoeba histolytica protein and 29 kda cysteine-rich protease. | amoebiasis is the third most common parasitic cause of morbidity and mortality, particularly in countries with poor hygienic settings. there exists an ambiguity in the diagnosis of amoebiasis, and hence there arises a necessity for a better diagnostic approach. serine-rich entamoeba histolyticaprotein (srehp), peroxiredoxin and gal/galnac lectin are pivotal in e. histolyticavirulence and are extensively studied as diagnostic and vaccine targets. for elucidating the cellular function of these pro ... | 2017 | 28674640 |
| x-ray snapshots of a pyridoxal enzyme: a catalytic mechanism involving concerted [1,5]-hydrogen sigmatropy in methionine γ-lyase. | pyridoxal 5'-phosphate (plp)-enzymes are essentially involved in amino acid and amine metabolism of a wide variety of organisms. despite their extensive biochemical studies, there are little evidence and structural data to comprehensively elaborate the catalytic mechanism. we obtained x-ray snapshots of l-methionine γ-lyase from entamoeba histolytica (ehmgl), a plp-enzyme catalyzing the γ-elimination reaction of methionine. here, we suggest a catalytic mechanism of ehmgl by using the x-ray snaps ... | 2017 | 28687762 |
| intestinal parasitic infections: current status and associated risk factors among school aged children in an archetypal african urban slum in nigeria. | intestinal parasitic infections (ipis) among school aged children (sac) in nigeria remains endemic, hence the need for regular surveillance to attract the attention of policy makers. this cross-sectional study investigated the current prevalence and factors associated with intestinal parasitic infections among school aged children in an urban slum of lagos city, nigeria. | 2017 | 28711437 |
| case report: endemic amebiasis in australia: implications for residents, travelers, and clinicians. | entamoeba histolytica is considered endemic in australia; however, cases are rare, occurring almost exclusively in high-risk individuals. we describe a series of locally acquired, complicated cases in low-risk individuals from far north queensland in whom the diagnosis was delayed. amebiasis may pose a greater local threat than is currently recognized. | 2017 | 28719303 |
| occult amoebic liver abscess as cause of extensive inferior vena cava and hepatic vein thrombosis. | the most common extraintestinal complication of entamoeba histolytica is amoebic liver abscess (ala). hepatic vein and inferior vena cava (ivc) thrombosis are rare but well-documented complications of ala, typically attributed to mechanical compression and inflammation associated with a large abscess. we present a case of a previously healthy 43-year-old canadian man presenting with constitutional symptoms and right upper quadrant abdominal pain. he was found to have thrombophlebitis of the ivc, ... | 2017 | 28722597 |
| investigation of pyrophosphate versus atp substrate selection in the entamoeba histolytica acetate kinase. | acetate kinase (ack; e.c. 2.7.2.1), which catalyzes the interconversion of acetate and acetyl phosphate, is nearly ubiquitous in bacteria but is present only in one genus of archaea and certain eukaryotic microbes. all acks utilize atp/adp as the phosphoryl donor/acceptor in the respective directions of the reaction (acetate + atp [formula: see text] acetyl phosphate + adp), with the exception of the entamoeba histolytica ack (ehack) which uses pyrophosphate (ppi)/inorganic phosphate (pi) (acety ... | 2017 | 28724909 |
| agc family kinase 1 participates in trogocytosis but not in phagocytosis in entamoeba histolytica. | the protozoan parasite entamoeba histolytica is the aetiologic agent of amoebiasis, an endemic infection in developing countries with considerable morbidity and mortality. recently, trogocytosis has been recognized as the key step in amoebic cytolysis and invasion, a paradigm shift in understanding pathogenicity of this organism. here we report that agc family kinase 1 is specifically involved in trogocytosis of live human cells and does not participate in phagocytosis of dead cells. live imagin ... | 2017 | 28740237 |
| use of serologic responses against enteropathogens to assess the impact of a point-of-use water filter: a randomized controlled trial in western province, rwanda. | diarrhea is a leading contributor to childhood morbidity and mortality in sub-saharan africa. given the challenge of blinding most water, sanitation, and hygiene (wash) interventions, diarrheal disease outcome measures in wash intervention trials are subject to potential bias and misclassification. using the platform of a cluster-randomized controlled trial of a household-based drinking water filter in western province, rwanda, we assessed the impact of the drinking water filter on enteric seroc ... | 2017 | 28749764 |
| comparison of hemolytic activity of the intermediate subunit of entamoeba histolytica and entamoeba dispar lectins. | galactose and n-acetyl-d-galactosamine-inhibitable lectin of entamoeba histolytica has roles in pathogenicity and induction of protective immunity in rodent models of amoebiasis. recently, the intermediate subunit of the lectin, igl1, of e. histolytica has been shown to have hemolytic activity. however, the corresponding lectin is also expressed in a non-virulent species, entamoeba dispar, and another subunit, igl2, is expressed in the protozoa. therefore, in this study, we compared the activiti ... | 2017 | 28750000 |
| self-reported infections during international travel and notifiable infections among returning international travellers, sweden, 2009-2013. | we studied food and water-borne diseases (fwds), sexually transmitted diseases (stds), vector-borne diseases (vbds) and diseases vaccinated against in the swedish childhood vaccination programme among swedish international travellers, in order to identify countries associated with a high number of infections. we used the national database for notifiable infections to estimate the number of fwds (campylobacteriosis, salmonellosis, giardiasis, shigellosis, ehec, entamoeba histolytica, yersinosis, ... | 2017 | 28753671 |
| differential expression of pathogenic genes of entamoeba histolytica vs e. dispar in a model of infection using human liver tissue explants. | we sought to establish an ex vivo model for examining the interaction of e. histolytica with human tissue, using precision-cut liver slices (pcls) from donated organs. e. histolytica- or e. dispar-infected pcls were analyzed at different post-infection times (0, 1, 3, 24 and 48 h) to evaluate the relation between tissue damage and the expression of genes associated with three factors: a) parasite survival (peroxiredoxin, superoxide dismutase and 70 kda heat shock protein), b) parasite virulence ... | 2017 | 28771523 |
| a unique case of empyema secondary to amoebic liver abscess. | an amoebic liver abscess is an extraintestinal manifestation of amoebiasis that can present with complaints such as right upper quadrant pain and fever. it might not necessarily be associated with abdominal complaints and can have many other atypical presentations. it may present with lung diseases, cardiac diseases, or brain abscesses. we present a case of a patient with empyema secondary to amoebic liver abscess whose diagnosis was delayed due to an unusual presentation. a combination of radio ... | 2017 | 28775917 |
| crystal structure of the peg-bound sh3 domain of myosin ib from entamoeba histolytica reveals its mode of ligand recognition. | the versatility in the recognition of various interacting proteins by the sh3 domain drives a variety of cellular functions. here, the crystal structure of the c-terminal sh3 domain of myosin ib from entamoeba histolytica (ehmysh3) is reported at a resolution of 1.7 å in native and peg-bound states. comparisons with other structures indicated that the peg molecules occupy protein-protein interaction pockets similar to those occupied by the peptides in other peptide-bound sh3-domain structures. a ... | 2017 | 28777082 |
| evaluation of the bd max enteric parasite panel for the detection of cryptosporidium parvum/hominis, giardia duodenalis and entamoeba histolytica. | conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. this study sought to evaluate the automated bd max enteric parasite panel (epp) for the detection of cryptosporidium parvum/hominis, entamoeba histolytica and giardia duodenalis.methodolgy. a total of 104 known positive samples (43 cryptosporidium parvum/hominis and 61 g. duodenalis), 15 simulated samples (e. histol ... | 2017 | 28792380 |
| larrea tridentata: a novel source for anti-parasitic agents active against entamoeba histolytica, giardia lamblia and naegleria fowleri. | protozoan parasites infect and kill millions of people worldwide every year, particularly in developing countries where access to clean fresh water is limited. among the most common are intestinal parasites, including giardia lamblia and entamoeba histolytica. these parasites wreak havoc on the epithelium lining the small intestines (g. lamblia) and colon (e. histolytica) causing giardiasis and amebiasis, respectively. in addition, there are less common but far more deadly pathogens such as naeg ... | 2017 | 28793307 |
| different behavior of myeloperoxidase in two rodent amoebic liver abscess models. | the protozoan entamoeba histolytica is the etiological agent of amoebiasis, which can spread to the liver and form amoebic liver abscesses. histological studies conducted with resistant and susceptible models of amoebic liver abscesses (alas) have established that neutrophils are the first cells to contact invasive amoebae at the lesion site. myeloperoxidase is the most abundant enzyme secreted by neutrophils. it uses hydrogen peroxide secreted by the same cells to oxidize chloride ions and prod ... | 2017 | 28796788 |
| outbreak of intestinal amoebiasis among men who have sex with men, barcelona (spain), october 2016 and january 2017. | entamoeba histolytica has been recently recognised as an emerging sexually transmissible pathogen in men who have sex with men (msm), causing sporadic outbreaks in countries where it is not endemic. here we report two closed clusters of invasive amoebiasis occurring in barcelona, spain, in october 2016 (four cases) and in january 2017 (four cases). | 2017 | 28797327 |
| gastrointestinal parasites in captive and free-ranging cebus albifrons in the western amazon, ecuador. | currently, there is a lack of surveys that report the occurrence of gastrointestinal parasites in the white-headed capuchin monkey (cebus albifrons). we therefore assessed the presence and richness (= number of different parasite genera) of parasites in c. albifrons in wildlife refuges (n = 11) and in a free-ranging group near a human village (n = 15) in the ecuadorian amazon. in the 78 samples collected (median of 3 samples per animal), we identified a total of 6 genera of gastrointestinal para ... | 2017 | 28808619 |
| microbiome-mediated neutrophil recruitment via cxcr2 and protection from amebic colitis. | the disease severity of entamoeba histolytica infection ranges from asymptomatic to life-threatening. recent human and animal data implicate the gut microbiome as a modifier of e. histolytica virulence. here we have explored the association of the microbiome with susceptibility to amebiasis in infants and in the mouse model of amebic colitis. dysbiosis occurred symptomatic e. histolytica infection in children, as evidenced by a lower shannon diversity index of the gut microbiota. to test if dysb ... | 2017 | 28817707 |
| in vitro testing of potential entamoeba histolytica pyruvate phosphate dikinase inhibitors. | adverse effects and resistance to metronidazole have motivated the search for new antiamoebic agents against entamoeba histolytica. control of amoeba growth may be achieved by inhibiting the function of the glycolytic enzyme and pyruvate phosphate dikinase (ppdk). in this study, we screened 10 compounds using an in vitro ppdk enzyme assay. these compounds were selected from a virtual screening of compounds in the national cancer institute database. the antiamoebic activity of the selected compou ... | 2017 | 28820699 |
| molecular analysis of the enteric protozoa associated with acute diarrhea in hospitalized children. | pediatric diarrhea is a common cause of death among children under 5 years of age. in the current study, we investigated the frequency of intestinal parasites among 580 pediatric patients with chronic diarrhea. parasitic protozoa (all species combined) were detected by molecular tools in 22.9% of the children and the most common parasite was cryptosporidium spp. (15.1%). blastocystis hominis was detected in 4.7%, dientamoeba fragilis in 4%, giardia duodenalis in 1.7%, and entamoeba histolytica i ... | 2017 | 28824878 |
| bioflow: a non-invasive, image-based method to measure speed, pressure and forces inside living cells. | cell motility is governed by a complex molecular machinery that converts physico-chemical cues into whole-cell movement. understanding the underlying biophysical mechanisms requires the ability to measure physical quantities inside the cell in a simple, reproducible and preferably non-invasive manner. to this end, we developed bioflow, a computational mechano-imaging method and associated software able to extract intracellular measurements including pressure, forces and velocity everywhere insid ... | 2017 | 28835648 |
| the macrophage cytoskeleton acts as a contact sensor upon interaction with entamoeba histolytica to trigger il-1β secretion. | entamoeba histolytica (eh) is the causative agent of amebiasis, one of the major causes of dysentery-related morbidity worldwide. recent studies have underlined the importance of the intercellular junction between eh and host cells as a determinant in the pathogenesis of amebiasis. despite the fact that direct contact and ligation between eh surface gal-lectin and ehcp-a5 with macrophage α5β1 integrin are absolute requirements for nlrp3 inflammasome activation and il-1β release, many other undef ... | 2017 | 28837696 |
| synthetic analogs of an entamoeba histolytica glycolipid designed to combat intracellular leishmania infection. | intracellular pathogens belonging to the genus leishmania have developed effective strategies that enable them to survive within host immune cells. immunostimulatory compounds that counteract such immunological escape mechanisms represent promising treatment options for diseases. here, we demonstrate that a lipopeptidephosphoglycan (lppg) isolated from the membrane of a protozoan parasite, entamoeba histolytica (eh), shows considerable immunostimulatory effects targeted against leishmania (l.) m ... | 2017 | 28842620 |
| evaluation of primitive ground water supplies as a risk factor for the development of major waterborne zoonosis in egyptian children living in rural areas. | endemic waterborne zoonosis frequently occurs in both developed and less developed countries. thus, bio-surveillance of waterborne zoonosis is a "necessity" for the implementation of effective preventive public health measures in egyptian rural areas. the primitive individual water supplies created by the rural agriculture population, primarily from ground water, usually maximize the customers' exposure to impurity pathogens via diffused humans and animal excreta or wastages. the current study a ... | 2017 | 28843417 |
| intestinal parasitic infections: current prevalence and risk factors among schoolchildren in capital area of the republic of marshall islands. | intestinal parasitic infections (ipis) among schoolchildren in republic of marshall islands (rmi) largely remains unknown, thus investigation on ipis status to establish the baseline data is urgently needed. this cross-sectional study intended to investigate the current ipis status and associated risk factors among schoolchildren at capital of rmi. single stool sample from 400 schoolchildren (207 boys and 193 girls) aged 9.73±2.50 yrs old was examined by employing merthiolate-iodine-formaldehyde ... | 2017 | 28847672 |
| inhibition of amebic lysosomal acidification blocks amebic trogocytosis and cell killing. | entamoeba histolytica ingests fragments of live host cells in a nibbling-like process termed amebic trogocytosis. amebic trogocytosis is required for cell killing and contributes to tissue invasion, which is a hallmark of invasive amebic colitis. work done prior to the discovery of amebic trogocytosis showed that acid vesicles are required for amebic cytotoxicity. in the present study, we show that acidified lysosomes are required for amebic trogocytosis and cell killing. interference with lysos ... | 2017 | 28851845 |
| antiprotozoal activity of extracts of elaeodendron trichotomum (celastraceae). | chagas disease, amebiasis, giardiasis and trichomoniasis represent a serious health problem in latin america. the drugs employed to treat these illnesses produce important side effects and resistant strains have appeared. the present study was aimed to evaluate the antiprotozoal activity of leaves, stem bark and root bark of elaeodendron trichotomum, a celastraceus, that is used in mexico as an anti-infective in febrile-type diseases. | 2016 | 28852732 |
| no detection of entamoeba histolytica by multiplex pcr in children with acute non-bloody diarrhea in guatemala. | entamoeba histolytica is believed to be a ubiquitous cause of pediatric non-bloody diarrhea. using a multiplex pcr assay, we identified stool pathogens in 298 guatemalan children presenting with acute, non-bloody diarrhea. despite a high overall pathogen burden, we identified no cases of e. histolytica. knowledge of local epidemiology is important to inform e. histolytica therapeutic strategies in low-income settings. | 2017 | 28858041 |