Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| is atomic rearrangement of type iv pha synthases responsible for increased pha production? | type iv pha synthase is a key enzyme responsible for catalyzing the formation of non-toxic, biocompatible, and biodegradable short-chain-length polyhydroxyalkanoates (scl-pha) under the growth-limiting conditions in the members of the genus bacillus. | 2015 | 25077376 |
| chemometric approach to prediction of antibacterial agent production by streptomyces hygroscopicus. | the nutritional requirements for antimicrobial agent production using streptomyces hygroscopicus were analyzed in shake flask experiments. antimicrobial activity was tested against staphylococcus aureus and bacillus cereus. the mathematical models have been generated with relative high complexity in order to give an adequate fit to the data. all the results suggest a high dependence of produced antimicrobial agent quantities on the amount of carbon, nitrogen, and phosphorus in cultivation medium ... | 2014 | 25082769 |
| secdf as part of the sec-translocase facilitates efficient secretion of bacillus cereus toxins and cell wall-associated proteins. | the aim of this study was to explore the role of secdf in protein secretion in bacillus cereus atcc 14579 by in-depth characterization of a markerless secdf knock out mutant. deletion of secdf resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. most toxins, including food poisoning-associated enterotoxins nhe, hbl, and cytotoxin k, as well as phospholipase c were less a ... | 2014 | 25083861 |
| inactivation of bacillus spores inoculated in milk by ultra high pressure homogenization. | ultra high-pressure homogenization treatments at 300 mpa with inlet temperatures (ti) of 55, 65, 75 and 85 °c were applied to commercial ultra high temperature treated whole milk inoculated with bacillus cereus, bacillus licheniformis, bacillus sporothermodurans, bacillus coagulans, geobacillus stearothermophilus and bacillus subtilis spores in order to evaluate the inactivation level achieved. ultra high-pressure homogenization conditions at 300 mpa with ti = 75 and 85 °c were capable of a spor ... | 2014 | 25084664 |
| incidence, diversity and characteristics of spores of psychrotolerant spore formers in various repfeds produced in belgium. | the major objectives of this study were to determine the incidence of psychrotolerant spore formers from repfeds marketed in belgium, and their diversity and characteristics. spore formers in general were found as spores on 38.3% of the food samples and in 85% food products types evaluated. 76% of the food samples containing spore formers had spores before enrichment. a total of 86 spore formers were isolated from the samples. 28 of 86 bacterial spore formers (32.6%) were capable of vegetative g ... | 2014 | 25084675 |
| potential of kalopanax septemlobus leaf extract in synthesis of silver nanoparticles for selective inhibition of specific bacterial strain in mixed culture. | silver nanoparticles (agnps) were synthesised using kalopanax septemlobus plant leaf extracts. uv-visible spectrophotometric, fourier-transform infrared, electron dispersive x-ray spectroscopy, scanning electron microscopy (sem) and transmission electron microscopy (tem) analyses confirmed synthesis of agnps. tem micrographs revealed presence of well-dispersed agnps predominantly of small size and different shapes with an average particle size of 30.8 nm. antimicrobial susceptibility tests of ag ... | 2014 | 25085530 |
| a laboratory case study of efficient polyhydoxyalkonates production by bacillus cereus, a contaminant in saccharophagus degradans atcc 43961 in minimal sea salt media. | a contaminating bacterium growing along with the stock culture of saccharophagus degradans atcc 43961 (sde 2-40) on marine agar plate was isolated and investigated for its ability to produce polyhydoxyalkonates (pha). preliminary screening by sudan black b and nile blue a staining indicated positive characteristic of the isolate to produce pha. the isolate was able to grow and produce pha in minimal sea salt medium broth. pha quantification studies with gas chromatographic analyses of the dry ce ... | 2014 | 25085545 |
| synthesis, spectroscopic, anticancer, antibacterial and antifungal studies of ni(ii) and cu(ii) complexes with hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene]. | schiff's base ligand(l) hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene] and its metal complexes have been synthesized and characterized by elemental analysis, molar conductance, various spectroscopic techniques such as electronic, ir, (1)h nmr, mass, epr. molar conductance of complexes in dmf solution corresponds to non-electrolyte. complexes have general composition [m(l)2x2], where m=ni(ii) and cu(ii), x=cl(-), no3(-), ch3coo(-) and ½so4(2-). on the basis of above spectral studies, an ... | 2015 | 25087168 |
| quorum sensing in bacillus thuringiensis is required for completion of a full infectious cycle in the insect. | bacterial cell-cell communication or quorum sensing (qs) is a biological process commonly described as allowing bacteria belonging to a same pherotype to coordinate gene expression to cell density. in gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors regulated by secreted and re-imported signaling peptides. the bacillus quorum sensors rap, nprr, and plcr were previously identified as the first members of a new protein family called rnpp. except for the rap prot ... | 2014 | 25089349 |
| cytotoxic and antibacterial quinone sesquiterpenes from a myrothecium fungus. | six new quinone sesquiterpenes, myrothecols a-f (1-6), and hymenopsin b (7) were isolated from cultures of myrothecium sp. sc0265 by bioassay-guided fractionation. their structures were elucidated on the basis of 1d and 2d nmr and ms data. the absolute configurations of the new compounds were assigned by cd/tddft calculations, and that of and hymenopsin b was confirmed by x-ray diffraction analysis. compounds 1-5 and hymenopsin demonstrated cytotoxic activity against human carcinoma a549, hela, ... | 2014 | 25089733 |
| efficacy of gaseous chlorine dioxide in inactivating bacillus cereus spores attached to and in a biofilm on stainless steel. | we evaluated the lethal activity of gaseous chlorine dioxide (clo2) against bacillus cereus spores attached to and in biofilm formed on a stainless steel surface. aqueous clo2 was prepared by mixing sulfuric acid (5% w/v) with sodium chlorite (10mg/ml), and gaseous clo2 was produced by vaporization of aqueous clo2 in an air-tight container. the concentration of gaseous clo2 in the air within the container increased rapidly at first but gradually decreased over time. the lethality of gaseous clo2 ... | 2014 | 25090607 |
| characterization of lipoteichoic acid structures from three probiotic bacillus strains: involvement of d-alanine in their biological activity. | probiotics represent a potential strategy to influence the host's immune system thereby modulating immune response. lipoteichoic acid (lta) is a major immune-stimulating component of gram-positive cell envelopes. this amphiphilic polymer, anchored in the cytoplasmic membrane by means of its glycolipid component, typically consists of a poly (glycerol-phosphate) chain with d-alanine and/or glycosyl substitutions. lta is known to stimulate macrophages in vitro, leading to secretion of inflammatory ... | 2014 | 25090957 |
| sub-emetic toxicity of bacillus cereus toxin cereulide on cultured human enterocyte-like caco-2 cells. | cereulide (cer) intoxication occurs at relatively high doses of 8 µg/kg body weight. recent research demonstrated a wide prevalence of low concentrations of cer in rice and pasta dishes. however, the impact of exposure to low doses of cer has not been studied before. in this research, we investigated the effect of low concentrations of cer on the behavior of intestinal cells using the caco-2 cell line. the mtt (mitochondrial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the s ... | 2014 | 25093386 |
| thiophene acetylenes and furanosesquiterpenes from xanthopappus subacaulis and their antibacterial activities. | in a search for naturally occurring antibacterial compounds in medicinal plants, six hitherto unknown thiophene acetylenes, named 10,11-threo-xanthopappin d, 10,11-erythro-xanthopappin d, 10,11-cis-xanthopappin b, 5-(but-4-chloro-3-hydroxy-1-ynyl)-2-(z)-pent-3-ene-1-ynylthiophene, 5-(but-4-chloro-3-hydroxy-1-ynyl)-2-(e)-pent-3-ene-1-ynylthiophene, 5-(but-3,4-dihydroxy-1-ynyl)-2-(z)-pent-3-ene-1-ynylthiophene and two furanosesquiterpenes, as well as fifteen known compounds, were isolated from xan ... | 2014 | 25096755 |
| the genomes, proteomes, and structures of three novel phages that infect the bacillus cereus group and carry putative virulence factors. | this article reports the results of studying three novel bacteriophages, jl, shanette, and basilisk, which infect the pathogen bacillus cereus and carry genes that may contribute to its pathogenesis. we analyzed host range and superinfection ability, mapped their genomes, and characterized phage structure by mass spectrometry and transmission electron microscopy (tem). the jl and shanette genomes were 96% similar and contained 217 open reading frames (orfs) and 220 orfs, respectively, while basi ... | 2014 | 25100842 |
| first synthesis of bacillus cereus ch hf-ps cell wall trisaccharide repeating unit. | the first total synthesis of ch hf-ps, a cell wall trisaccharide repeating unit of b. cereus, is reported. the synthetic trisaccharide is appended with an aminopropyl linker at the reducing end to allow for conjugation to proteins and microarrays. the convergent synthesis involves transformation of d-mannose into an orthogonally protected rare aat sugar building block, two consecutive α-stereoselective glycosylations, β-selective attachment of the linker by solvent participation, and amide bond ... | 2014 | 25101800 |
| antimicrobial and allelopathic metabolites produced by penicillium brasilianum. | six known compounds, isoroquefortine c (1), griseofulvin (2), ergosterol peroxide (3), 3β-hydroxy-(22e,24r)-ergosta-5,8,22-trien-7-one (4), cerevisterol (5) and (22e,24r)-6β-methoxyergosta-7,22-diene-3β,5α-diol (6), were produced by the fungus penicillium brasilianum, and their structures were elucidated by spectroscopic methods. this is the first report on isoroquefortine c as naturally occurring compound. their bioactivities against five phytopathogenic fungi (gibeberalla saubinetti, fusarium ... | 2015 | 25103127 |
| antibacterial tetraoxygenated xanthones from the immature fruits of garcinia cowa. | a phytochemical investigation of the acetone extract from the immature fruits of garcinia cowa led to the isolation of two novel tetraoxygenated xanthones, garcicowanones a (1) and b (2), together with eight known tetraoxygeanted xanthones. their structures were determined by spectroscopic analysis. all isolated compounds were evaluated for their antibacterial activity against bacillus cereus tistr 688, bacillus subtilis tistr 008, micrococcus luteus tistr 884, staphylococcus aureus tistr 1466, ... | 2014 | 25110196 |
| comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk. | psychrotrophic bacteria in raw milk produce heat-resistant extracellular proteases, resulting in spoilage and shelf-life reduction of ultrahigh temperature treated milk and milk products. controlling of these spoilage microbes requires rapid and reliable identification systems for screening of raw milk. this study aimed to compare commercial bacterial identification systems with a genetic method (considered as the 'gold standard' method) for the identification of heat-resistant protease producin ... | 2014 | 25113043 |
| modular genetic architecture of the toxigenic plasmid pis56-63 harboring cry1ab21 in bacillus thuringiensis subsp. thuringiensis strain is5056. | bacillus thuringiensis subsp. thuringiensis is5056, a strain highly toxic to trichoplusia ni larvae, produces the newly described cry1ab21 delta-endotoxin encoded by a gene located in the 63.8 kb pis56-63 plasmid. in this report we present the structure and functional similarity of this plasmid to other b. thuringiensis large toxigenic plasmids with particular interest focused on its modular architecture. the 61 open reading frames (orfs) of the plasmid made four functional modules: (i) m1-mic, ... | 2014 | 25115108 |
| volatile constituents of dianthus rupicola biv. from sicily: activity against microorganisms affecting cellulosic objects. | dianthus rupicola biv. (cliffs carnation) is a camephytic, suffruticous, perennial plant growing up to 40 cm high. the plant is widespread in sicily and neighbouring islands (egadi, lampedusa, lipari) and in some areas of southern italy. gc and gc-ms analyses of the essential oil distilled from the flowers showed the presence of 66 components. its composition is characterised by the high content of thymol and carvacrol derivatives. a good antibacterial activity against bacillus cereus and bacill ... | 2014 | 25115728 |
| mangostanaxanthones i and ii, new xanthones from the pericarp of garcinia mangostana. | two new xanthones: mangostanaxanthones i (3) and ii (5) were isolated from the pericarp of garcinia mangostana, along with four known xanthones: 9-hydroxycalabaxanthone (1), parvifolixanthone c (2), α-mangostin (4), and rubraxanthone (6). their structures were elucidated on the basis of ir, uv, 1d, 2d nmr, and ms spectroscopic data, in addition to comparison with literature data. the isolated compounds were evaluated for their antioxidant, antimicrobial, and quorum-sensing inhibitory activities. ... | 2014 | 25128900 |
| [effects of inoculating plant growth-promoting rhizobacteria on the biological characteristics of walnut (juglans regia) rhizosphere soil under drought condition]. | effects of four plant growth-promoting rhizobacteria (pgpr) , namely pseudomonas sp. yt3, bacillus subtilis dz1, b. cereus l90 and b. fusiformis l13 on the biological characteristics of walnut (juglans regia) rhizosphere soil under drought stress were investigated. results showed that drought stress had little effect on available nutrients of walnut rhizosphere soil, but significantly decreased the activity of organic carbon by 18.4% and increased the ph from 7.34 to 7.79. under drought stress c ... | 2014 | 25129951 |
| effect of crude extracts of selected actinomycetes on biofilm formation of a. schindleri, m. aci, and b. cereus. | actinomycetes are well known group of gram positive bacteria for their potential to produce antibiotics. this study sought to assess the ability of the selected actinomycetes to control biofilm forming bacteria isolated from different dental plaque samples. on the basis of morphological differences three out of ten different dental plaque bacterial isolates were selected for further study. these isolates were biochemically and genetically characterized and were identified as acinetobacter schinn ... | 2015 | 25138589 |
| secondary metabolites: applications on cultural heritage. | biological sciences and related bio-technology play a very important role in research projects concerning protection and preservation of cultural heritage for future generations. in this work secondary metabolites of burkholderia gladioli pv. agaricicola (bga) icmp 11096 strain and crude extract of glycoalkaloids from solanaceae plants, were tested against a panel of microorganisms isolated from calcarenite stones of two historical bridges located in potenza and in campomaggiore (southern italy) ... | 2013 | 25145230 |
| antibacterial and antifungal activity of holothuria leucospilota isolated from persian gulf and oman sea. | emergence of antimicrobial resistance toward a number of conventional antibiotics has triggered the search for antimicrobial agents from a variety of sources including the marine environment. | 2014 | 25147657 |
| the antibacterial activity of cassia fistula organic extracts. | cassia fistula, is a flowering plant and a member of fabaceae family. its leaves are compound of 4 - 8 pairs of opposite leaflets. there are many cassia species around the world which are used in herbal medicine. | 2014 | 25147664 |
| biodegradation of hard keratins by two bacillus strains. | extensive quantities of keratinic by-products are disposed annually by animal-processing industry, causing a mounting ecological problem due to extreme resilience of these materials to enzymatic breakdown. there is a growing trend to apply cheap and environment-friendly methods to recycle keratinic wastes. soil bacteria of profound keratinolytic potential, especially spore-forming rods from the genus bacillus, play a significant role in keratinase-mediated biodegradation of keratins, therefore c ... | 2014 | 25147672 |
| antimicrobial and genotoxicity effects of zero-valent iron nanoparticles. | in a world of nanotechnology, the first concern is the potential environmental impact of nanoparticles. an efficient way to estimate nanotoxicity is to monitor the responses of bacteria exposed to these particles. | 2014 | 25147712 |
| collagen-like glycoprotein bcls is involved in the formation of filamentous structures of the lysinibacillus sphaericus exosporium. | lysinibacillus sphaericus produces mosquitocidal binary toxins (bin toxins) deposited within a balloon-like exosporium during sporulation. unlike bacillus cereus group strains, the exosporium of l. sphaericus is usually devoid of the hair-like nap, an external filamentous structure formed by a collagen-like protein, bcla. in this study, a new collagen-like exosporium protein encoded by bsph_0411 (bcls) from l. sphaericus c3-41 was characterized. thin-section electron microscopy revealed that del ... | 2014 | 25149519 |
| cobalt(ii) complexes with the antimicrobial drug enrofloxacin: structure, antimicrobial activity, dna- and albumin-binding. | the cobalt(ii) complexes with the quinolone antimicrobial agent enrofloxacin (herx) in the presence of the nitrogen-donor heterocyclic ligands pyridine (py), 2,2'-bipyridylamine (bipyam), 1,10-phenanthroline (phen), 2,2'-bipyridine (bipy) or the oxygen-donor ligand n,n-dimethylformamide (dmf) were synthesized and characterized. the crystal structures of complexes [co(erx)2(py)2]·meoh·6h2o and [co(erx)2(bipyam)]·4.5meoh·1.25h2o were determined by x-ray crystallography. the deprotonated enrofloxac ... | 2014 | 25151581 |
| biocontrol of aspergillus species on peanut kernels by antifungal diketopiperazine producing bacillus cereus associated with entomopathogenic nematode. | the rhabditid entomopathogenic nematode associated bacillus cereus and the antifungal compounds produced by this bacterium were evaluated for their activity in reducing postharvest decay of peanut kernels caused by aspergillus species in in vitro and in vivo tests. the results showed that b. cereus had a significant effect on biocontrol effectiveness in in vitro and in vivo conditions. the antifungal compounds produced by the b. cereus were purified using silica gel column chromatography and the ... | 2014 | 25157831 |
| thermal inactivation parameters of spores from different phylogenetic groups of bacillus cereus. | the thermal inactivation kinetics of spores from 39 bacillus cereus strains belonging to six different phylogenetic groups (group ii to vii) was studied. fresh spore suspensions in glass capillaries were heated in an oil bath at three or more different temperatures for five different times. survival curves and thermal death curves were established and the kinetic parameters dt (decimal reduction time at temperature t) and z (temperature dependence of dt) were derived by linear regression. most s ... | 2014 | 25171111 |
| cspa encodes a major cold shock protein in himalayan psychrotolerant pseudomonas strains. | the major cold-shock protein (cspa) encoding gene cspa were detected in three himalayan psychrotrophic pseudomonad strains, by pcr amplification. partial sequencing of three pseudomonas strains cspa gene and blast search confirmed the high similarity with putative bacterial cspa gene and bacterial cspa protein. bioinformatics analysis of these partial cspa amino acid sequences showed presence of putative conserved region for dna/rna-binding motifs rnp-1 and rnp-2. protein homologies of all three ... | 2014 | 25172452 |
| analgesic, antibacterial and central nervous system depressant activities of albizia procera leaves. | to ascertain analgesic, antibacterial and central nervous system (cns) depressant activities of ethyl acetate, dichloromethane and carbon tetrachloride fractions of methanol extract of albizia procera (a. procera) leaves. | 2014 | 25182551 |
| characterization of three bacillus cereus strains involved in a major outbreak of food poisoning after consumption of fermented black beans (douchi) in yunan, china. | three bacillus cereus strains isolated from an outbreak of food poisoning caused by the consumption of fermented black beans (douchi) containing b. cereus is described. the outbreak involved 139 persons who had nausea, vomiting, and diarrhea. the strains were isolated from vomit and the unprepared douchi. two of the strains produced the emetic toxin cereulide, as evidenced by polymerase chain reaction analysis for the presence of the nonribosomal synthetase cluster responsible for the synthesis ... | 2014 | 25188780 |
| enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain). | in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ... | 2014 | 25193866 |
| structural data and biological properties of almond gum oligosaccharide: application to beef meat preservation. | enzymatic hydrolysis of almond gum generates low molecular weight oligosaccharides (oag) with a yield of 33.5%. the generated oligosaccharides were purified and identified. oag analyses show that the most prominent residues were galactose and arabinose with traces of xylose, rhamnose, glucose and mannose. the glycosyl linkage positions were analyzed using gas chromatography-mass spectrometry showing a main chain composed of galactose units [ → 3)-gal-(1 → ] branched mainly with arabinose residue ... | 2015 | 25195541 |
| purification, crystallization and preliminary x-ray crystallographic analysis of glycosyltransferase-1 from bacillus cereus. | glycosyltransferases (gts), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. glycosyltransferase-1 from bacillus cereus (bcgt-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (udp-glucose) as the sugar donor. bcgt-1 (molecular mass 45.5 kda) ... | 2014 | 25195897 |
| simultaneous efficient removal of high-strength ammonia nitrogen and chemical oxygen demand from landfill leachate by using an extremely high ammonia nitrogen-resistant strain. | bioaugmentation is a promising technology for pollutant elimination from stressed environments, and it would provide an efficient way to solve challenges in traditional biotreatment of wastewater with high strength of ammonia nitrogen (nh4(+)-n). a high nh4(+)-n-resistant bacteria strain, identified as bacillus cereus (jlu bc), was domesticated and isolated from the bacteria consortium in landfill leachate. jlu bc could survive in 100 g/l nh4(+)-n environment, which indicated its extremely high ... | 2015 | 25196098 |
| hs-spme-gc-fid method for detection and quantification of bacillus cereus atcc 10702 mediated 2-acetyl-1-pyrroline. | a rapid micro-scale solid-phase micro-extraction (spme) procedure coupled with gas-chromatography with flame ionized detector (gc-fid) was used to extract parts per billion levels of a principle basmati aroma compound "2-acetyl-1-pyrroline" (2-ap) from bacterial samples. in present investigation, optimization parameters of bacterial incubation period, sample weight, pre-incubation time, adsorption time, and temperature, precursors and their concentrations has been studied. in the optimized condi ... | 2014 | 25196224 |
| high-quality draft genome sequence of nematocidal bacillus thuringiensis sbt003. | bacillus thuringiensis represents one of the six species of "bacillus cereus group" in the genus bacillus within the family bacillaceae. strain sbt003 was isolated from soil and identified as b. thuringiensis. it harbors at least seven plasmids and produces three shapes of parasporal crystals including oval, bipyramidal and rice. sds-page analysis of spore-crystal suspension of this strain reveals six major protein bands, which implies the presence of multiple parasporal crystal genes. bioassay ... | 2014 | 25197449 |
| clostridium and bacillus binary enterotoxins: bad for the bowels, and eukaryotic being. | some pathogenic spore-forming bacilli employ a binary protein mechanism for intoxicating the intestinal tracts of insects, animals, and humans. these gram-positive bacteria and their toxins include clostridium botulinum (c2 toxin), clostridium difficile (c. difficile toxin or cdt), clostridium perfringens (ι-toxin and binary enterotoxin, or bec), clostridium spiroforme (c. spiroforme toxin or cst), as well as bacillus cereus (vegetative insecticidal protein or vip). these gut-acting proteins for ... | 2014 | 25198129 |
| bacteriological quality of vegetables from organic and conventional production in different areas of korea. | foods grown in organic production systems have been described as representing an increased risk to public health compared with foods from conventional production. leafy vegetables (spinach, romaine lettuce, and green sesame leaves) grown in organic and conventional systems were collected from various areas in korea and examined using standard culture methods to compare the microbiological quality of the produce grown in the two agricultural systems. the 354 samples of these leafy vegetables were ... | 2014 | 25198606 |
| reducing pathogens by using zinc oxide nanoparticles and acetic acid in sheep meat. | practical applications of different concentrations (0, 1, 2, 4, 6, and 8 mm) of zinc oxide (zno) suspensions containing 1 % acetic acid were investigated against the pathogenic bacteria listeria monocytogenes, escherichia coli, staphylococcus aureus, and bacillus cereus. zno suspensions (0, 1, 3, 6, and 8 mm) containing acetic acid had a significant inhibitory effect on the growth of l. monocytogenes, e. coli, and s. aureus during 12 h of incubation, and the 8 mm suspensions of zno were the most ... | 2014 | 25198854 |
| proteomic evidences for rex regulation of metabolism in toxin-producing bacillus cereus atcc 14579. | the facultative anaerobe, bacillus cereus, causes diarrheal diseases in humans. its ability to deal with oxygen availability is recognized to be critical for pathogenesis. the b. cereus genome comprises a gene encoding a protein with high similarities to the redox regulator, rex, which is a central regulator of anaerobic metabolism in bacillus subtilis and other gram-positive bacteria. here, we showed that b. cereus rex is monocistronic and down-regulated in the absence of oxygen. the protein en ... | 2014 | 25216269 |
| biological activity of pinus nigra terpenes--evaluation of ftsz inhibition by selected compounds as contribution to their antimicrobial activity. | in the current work, in vitro antioxidant, antibacterial, and antifungal activites of the needle terpenes of three taxa of pinus nigra from serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed. the black pine essential oils showed generally weak antioxidative properties tested by two methods (dpph and abts scavenging assays), where the highest activity was identified in p. nigra var. banatica (ic50=25.08 mg/ml and vitc=0.67 mg (vitamin c)/g when tested with the dpph and abts rea ... | 2014 | 25217763 |
| potentiality of eisenia fetida to degrade disposable paper cups-an ecofriendly solution to solid waste pollution. | the aim of the present study was to subject the post-consumer waste, namely paper cups for vermicomposting along with cow dung in three different ratios for a period of 90-140 days employing eisenia fetida. the post-consumer wastes are a menace in many developing countries including india. this waste was provided as feed for earthworms and was converted to vermicompost. vermicompost prepared with paper cup waste was analyzed for their physicochemical properties. based on the physicochemical prop ... | 2015 | 25220767 |
| two rhizobacterial strains, individually and in interactions with rhizobium sp., enhance fusarial wilt control, growth, and yield in pigeon pea. | a pseudomonas aeruginosa strain, rrlj 04, and a bacillus cereus strain, bs 03, were tested both individually and in combination with a rhizobium strain, rh 2, for their ability to enhance plant growth and nodulation in pigeon pea (cajanus cajan l.) under gnotobiotic, greenhouse and field conditions. both of the rhizobacterial strains exhibited a positive effect on growth in terms of shoot height, root length, fresh and dry weight, nodulation and yield over the non-treated control. co-inoculation ... | 2014 | 25224506 |
| isolation and characterization of flagellar filaments from bacillus cereus atcc 14579. | isolated flagellar filaments from the type strain of bacillus cereus, atcc 14579, were shown to consist of 34, 32 and 31 kda proteins in similar proportions as judged by band intensities on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the n-terminal amino acid sequences of these three proteins of strain atcc 14579 were identical with the deduced sequences of three flagellin genes bc1657, bc1658 and bc1659 in the whole genome sequence. strain atcc 14579 was classified into serotype ... | 2014 | 25227778 |
| implementation of the bacillus cereus microbiological plate used for the screening of tetracyclines in raw milk samples with star protocol - the problem with false-negative results solved. | in antibiotic residue analyses the first step of screening is just as important as the following steps. screening methods need to be quick and inexpensive, but above all sensitive enough to detect the antibiotic residue at or below the maximum residue limit (mrl). in the case of a positive result, the next step is conducted and further methods are used for confirmation. mrls stated in european union regulation 37/2010 for tetracyclines in raw milk are: 100 µg kg(-1) for tetracycline, 100 µg kg(- ... | 2014 | 25230820 |
| murine model of bacillus cereus gastrointestinal infection. | bacillus cereus is a spore-forming micro-organism responsible for foodborne illness. in this study, we focus on the host response following intragastric challenge with a pathogenic b. cereus strain (b10502) isolated from a foodborne outbreak. c57bl/6j female mice were infected by gavage with strain b10502. controls were administered with pbs. infection leads to significant modification in relevant immune cells in the spleen, peyer's patches (pp) and mesenteric lymph nodes (mln). these findings c ... | 2014 | 25231625 |
| antimicrobial activity of antarctic bryozoans: an ecological perspective with potential for clinical applications. | the antimicrobial activity of antarctic bryozoans and the ecological functions of the chemical compounds involved remain largely unknown. to determine the significant ecological and applied antimicrobial effects, 16 ether and 16 butanol extracts obtained from 13 different bryozoan species were tested against six antarctic (including psychrobacter luti, shewanella livingstonensis and 4 new isolated strains) and two bacterial strains from culture collections (escherichia coli and bacillus cereus). ... | 2014 | 25232675 |
| comparison of hand hygiene procedures for removing bacillus cereus spores. | bacillus cereus is a spore-forming bacterium. b. cereus occasionally causes nosocomial infections, in which hand contamination with the spores plays an important role. therefore, hand hygiene is the most important practice for controlling nosocomial b. cereus infections. this study aimed to determine the appropriate hand hygiene procedure for removing b. cereus spores. thirty volunteers' hands were experimentally contaminated with b. cereus spores, after which they performed 6 different hand hyg ... | 2014 | 25252644 |
| phosphatase-inert glucosamine 6-phosphate mimics serve as actuators of the glms riboswitch. | the glms riboswitch is unique among gene-regulating riboswitches and catalytic rnas. this is because its own metabolite, glucosamine-6-phosphate (glcn6p), binds to the riboswitch and catalytically participates in the rna self-cleavage reaction, thereby providing a novel negative feedback mechanism. given that a number of pathogens harbor the glms riboswitch, artificial actuators of this potential rna target are of great interest. structural/kinetic studies point to the 2-amino and 6-phosphate es ... | 2014 | 25254431 |
| development of biopolymer nanocomposite for silver nanoparticles and ciprofloxacin controlled release. | screening of biopolymeric gel beads containing silver nanoparticles (ag-nps) stabilized in guar gum alkyl amine (ggaa) and ciprofloxacin (cip) was carried out in order to obtain a novel nanocomposite with controlled release profile of both antimicrobians. the selected matrix composed of alginate/high methoxyl pectin (hmp)/ggaa (4:4:1) was able to co-incorporate ag-nps and cip with encapsulation efficiency higher than 70%. sem images revealed good cohesivity and compatibility between the biopolym ... | 2015 | 25256547 |
| terpenoids, flavonoids and caffeic acid derivatives from salvia viridis l. cvar. blue jeans. | three diterpenoids, 1-oxomicrostegiol (1), viroxocin (2), viridoquinone (3), were isolated from the roots of salvia viridis l. cvar. blue jeans. five known diterpenoids, microstegiol (4), 7α-acetoxy-14-hydroxy-8,13-abietadiene-11,12-dione (5; 7-o-acetylhorminone tautomer), 7α,14-dihydroxy-8,13-abietadiene-11,12-dione (6; horminone tautomer), ferruginol and salvinolonyl 12-methyl ether (7) were also found in the roots together with 1-docosyl ferulate (8), and a mixture of 2-(4'-alkoxyphenyl) ethy ... | 2014 | 25256822 |
| bioaugmentation of a sequencing batch biofilm reactor with comamonas testosteroni and bacillus cereus and their impact on reactor bacterial communities. | the immobilization of microorganisms is essential for efficient bioaugmentation systems. the performance of bacillus cereus g5 as biofilm-forming bacteria and comamonas testosteroni a3 a 3,5 dinitrobenzoic acid (dnb)-degrading strain] in laboratory-scale sequencing batch biofilm reactors (sbbrs) treating dnb synthetic wastewater has been examined. the microbial diversity in the reactors was also explored. the reactor r3 inoculated with b. cereus g5 and c. testosteroni a3 together not only improv ... | 2015 | 25257599 |
| influence of lysogeny of tectiviruses gil01 and gil16 on bacillus thuringiensis growth, biofilm formation, and swarming motility. | bacillus thuringiensis is an entomopathogenic bacterium that has been used as an efficient biopesticide worldwide. despite the fact that this bacterium is usually described as an insect pathogen, its life cycle in the environment is still largely unknown. b. thuringiensis belongs to the bacillus cereus group of bacteria, which has been associated with many mobile genetic elements, such as species-specific temperate or virulent bacteriophages (phages). temperate (lysogenic) phages are able to est ... | 2014 | 25261525 |
| isolation and characterization of a furfural-degrading bacterium bacillus cereus sp. strain ds1. | furfural was found to be the main organic pollutant in the wastewater coming from the diosgenin factory. this substance is derived from acidic pentosan in dioscorea zingiberensis and is also found in a variety of agricultural byproducts, including corncobs, oat, wheat bran, and sawdust. it is regarded as a toxicant and an inhibitor to the growth of microorganism in both sewage disposal and biological fermentation. a furfural-degrading strain (ds1) was isolated from activated sludge of wastewater ... | 2015 | 25274411 |
| genomic comparison of 93 bacillus phages reveals 12 clusters, 14 singletons and remarkable diversity. | the bacillus genus of firmicutes bacteria is ubiquitous in nature and includes one of the best characterized model organisms, b. subtilis, as well as medically significant human pathogens, the most notorious being b. anthracis and b. cereus. as the most abundant living entities on the planet, bacteriophages are known to heavily influence the ecology and evolution of their hosts, including providing virulence factors. thus, the identification and analysis of bacillus phages is critical to underst ... | 2014 | 25280881 |
| identification of multidrug-resistant bacteria and bacillus cereus from healthcare workers and environmental surfaces in a hospital. | 2014 | 25280977 | |
| prevalence and characterization of foodborne pathogens from australian dairy farm environments. | the ability of foodborne pathogens to gain entry into food supply systems remains an ongoing concern. in dairy products, raw milk acts as a major vehicle for this transfer; however, the sources of pathogenic bacteria that contaminate raw milk are often not clear, and environmental sources of contamination or the animals themselves may contribute to the transfer. this survey examined the occurrence of 9 foodborne pathogens in raw milk and environments of 7 dairy farms (3 bovine, 3 caprine, and 1 ... | 2014 | 25282417 |
| antioxidant and antibacterial activities of exopolysaccharides from bifidobacterium bifidum wbin03 and lactobacillus plantarum r315. | the objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (eps) from bifidobacterium bifidum wbin03 (b-eps) and lactobacillus plantarum r315 (l-eps). the 1,1-diphenyl-2-picrylhydrazyl (dpph)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. both b-eps and l-eps had strong sca ... | 2014 | 25282420 |
| [bacillus cereus septicemia and necrotizing fasciitis in a patient with liver cirrhosis: a case report]. | a 54-year-old woman with hematemesis was referred to our hospital. she had a history of liver cirrhosis and diabetes mellitus. after inserting a sengstaken-blakemore tube, we performed endoscopic variceal ligation for ruptured esophageal varices. on the third day of admission, she developed septicemia and necrotizing fasciitis caused by bacillus cereus. she was successfully treated with early debridement of both lower extremities and intravenous treatment with vancomycin, ciprofloxacin, and clin ... | 2014 | 25283231 |
| thiolation-enhanced substrate recognition by d-alanyl carrier protein ligase dlta from bacillus cereus. | d-alanylation of the lipoteichoic acid on gram-positive cell wall is dependent on dlt gene-encoded proteins dlta, dltb, dltc and dltd. the d-alanyl carrier protein ligase dlta, as a remote homolog of acyl-(coenzyme a) (coa) synthetase, cycles through two active conformations for the catalysis of adenylation and subsequent thiolation of d-alanine (d-ala). the crystal structure of dlta in the absence of any substrate was observed to have a noticeably more disordered pocket for atp which would expl ... | 2014 | 25285205 |
| purification and characterization of a mucin specific mycelial lectin from aspergillus gorakhpurensis: application for mitogenic and antimicrobial activity. | lectins are carbohydrate binding proteins or glycoproteins that bind reversibly to specific carbohydrates present on the apposing cells, which are responsible for their ability to agglutinate red blood cells, lymphocytes, fibroblasts, etc. interest in lectins has been intensified due to their carbohydrate specificity as they can be valuable reagents for the investigation of cell surface sugars, purification and characterization of glycoproteins. the present study reports the purification, charac ... | 2014 | 25286160 |
| biological control of meloidogyne hapla using an antagonistic bacterium. | we examined the efficacy of a bacterium for biocontrol of the root-knot nematode (rkn) meloidogyne hapla in carrot (daucus carota subsp. sativus) and tomato (solanum lycopersicum). among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate c1-7, which was identified as bacillus cereus based on cultural and morphological characteristics, the biolog program, and 16s rrna sequencing analyses. the population density and the nem ... | 2014 | 25289015 |
| increased activity of goat liver plasma membrane alkaline phosphatase upon release by phosphatidylinositol-specific phospholipase c. | mammalian alkaline phosphatase (alp) is attached to the plasma membrane by a unique glycosylphosphatidylinositol (gpi) anchor. the influence of such a complex anchoring device on the enzyme function is not fully understood. here, we report the effect of cleavage of the gpi anchor on the activity of goat liver plasma membrane alp (glpm-alp). phosphatidylinositol-specific phospholipase c (pi-plc) purified from bacillus cereus was used for the cleavage of the gpi anchor (delipidation) and hence for ... | 2014 | 25296497 |
| in vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from spilanthes paniculata leaves. | the study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of spilanthes paniculata leaves for the first time widely used in the traditional treatments in bangladesh. | 2014 | 25299748 |
| draft genome sequence of ft9, a novel bacillus cereus strain isolated from a brazilian thermal spring. | a bacillus cereus strain, ft9, isolated from a hot spring in the midwest region of brazil, had its entire genome sequenced. | 2014 | 25301660 |
| inhibition of bacillus cereus strains by antimicrobial metabolites from lactobacillus johnsonii crl1647 and enterococcus faecium sm21. | bacillus cereus is an endospore-forming, gram-positive bacterium able to cause foodborne diseases. lactic acid bacteria (lab) are known for their ability to synthesize organic acids and bacteriocins, but the potential of these compounds against b. cereus has been scarcely documented in food models. the present study has examined the effect of the metabolites produced by lactobacillus johnsonii crl1647 and enterococcus faecium sm21 on the viability of select b. cereus strains. furthermore, the ef ... | 2014 | 25305011 |
| chemical composition and antimicrobial activity of polish herbhoneys. | the present study focuses on samples of polish herbhoneys (hhs), their chemical composition and antimicrobial activity. a gas chromatography-mass spectrometry (gc-ms) method was used to analyse eight samples of herbal honeys and three samples of nectar honeys. their antimicrobial activities were tested on selected gram-positive (bacillus cereus, staphylococcus aureus, staphylococcus schleiferi) and gram-negative (escherichia coli) bacteria, as well as on pathogenic fungi candida albicans. ether ... | 2015 | 25308646 |
| enterotoxigenic profiling of emetic toxin- and enterotoxin-producing bacillus cereus, isolated from food, environmental, and clinical samples by multiplex pcr. | bacillus cereus comprises the largest group of endospore-forming bacteria and can cause emetic and diarrheal food poisoning. a total of 496 b. cereus strains isolated from various sources (food, environmental, clinical) were assessed by a multiplex pcr for the presence of enterotoxin genes. the detection rate of nhea, entfm, hblc, and cytk enterotoxin genes among all b. cereus strains was 92.33%, 77.21%, 59.47%, and 47.58%, respectively. enterotoxigenic profiles were determined in emetic toxin- ... | 2014 | 25311736 |
| antibacterial activity of ethyl acetate and aqueous extracts of mentha longifolia l. and hydroalcoholic extract of zataria multiflora boiss. plants against important human pathogens. | to determine the potential antibacterial activity of ethyl acetate and aqueous extracts from mentha longifolia l. (m. longifolia) and hydroalcoholic extract of zataria multiflora boiss. (z. multiflora) against important human pathogens. | 2014 | 25312118 |
| chemical compositions and antimicrobial activities of athrixia phylicoides dc. (bush tea), monsonia burkeana (special tea) and synergistic effects of both combined herbal teas. | to determine the chemical compositions and evaluate the antimicrobial activity of bush tea (athrixia phylicoides dc.), special tea (monsonia burkeana) and synergy (combination of bush tea and special tea). | 2014 | 25312166 |
| an in vitro antibacterial and antifungal effects of cadmium(ii) complexes of hexamethyltetraazacyclotetradecadiene and isomers of its saturated analogue. | to evaluate the antibacterial and antifungal effects of cadmium(ii) complexes with hexamethyltetraazacyclotetradecadiene ligands. | 2014 | 25312179 |
| immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated escherichia coli and bacillus cereus on holoxenic mice. | competition of probiotic bacteria with other species from the intestinal microbiota involves different mechanisms that occur regardless of probiotics' viability. the objective of this paper was to assess the cytokine serum levels in holoxenic mice after oral administration of non-viable components (nvc) of enterococcus faecium probiotic culture stimulated with heat-inactivated escherichia coli and bacillus cereus in comparison to nvc of unstimulated e. faecium probiotic culture. | 2014 | 25317114 |
| plant growth promoting bacteria from cow dung based biodynamic preparations. | indigenous formulations based on cow dung fermentation are commonly used in organic farming. three biodynamic preparations viz., panchagavya (pg), bd500 and 'cow pat pit' (cpp) showed high counts of lactobacilli (10(9) ml(-1)) and yeasts (10(4) ml(-1)). actinomycetes were present only in cpp (10(4) ml(-1)) and absent in the other two. seven bacterial isolates from these ferments were identified by a polyphasic approach: bacillus safensis (pg1), bacillus cereus (pg2, pg4 pg5), bacillus subtilis ( ... | 2014 | 25320439 |
| lifesaving liver transplantation for multi-organ failure caused by bacillus cereus food poisoning. | bacillus cereus is a spore-forming, gram-positive bacterium that causes food poisoning presenting with either emesis or diarrhea. diarrhea is caused by proteinaceous enterotoxin complexes, mainly hemolysin bl, non-hemolytic enterotoxin (nhe), and cytotoxin k. in contrast, emesis is caused by the ingestion of the depsipeptide toxin cereulide, which is produced in b. cereus contaminated food, particularly in pasta or rice. in general, the illness is mild and self-limiting. however, due to cereulid ... | 2015 | 25323120 |
| synthesis, characterization and antibacterial activity of biodegradable films prepared from schiff bases of zein. | pure zein is known to be very hydrophobic, but is still inappropriate for coating and film applications because of their brittle nature. in an attempt to improve the flexibility and the antimicrobial activity of these coatings and films, chemical modification of zein through forming schiff bases with different phenolic aldhydes was tried. influence of this modifications on mechanical, topographical, wetting properties and antimicrobial activity of zein films were evaluated. the chemical structur ... | 2014 | 25328181 |
| divergence of protein-coding capacity and regulation in the bacillus cereus sensu lato group. | the bacillus cereus sensu lato group contains ubiquitous facultative anaerobic soil-borne gram-positive spore-forming bacilli. molecular phylogeny and comparative genome sequencing have suggested that these organisms should be classified as a single species. while clonal in nature, there do not appear to be species-specific clonal lineages, excepting b. anthracis, in spite of the wide array of phenotypes displayed by these organisms. | 2014 | 25350501 |
| bacillus subtilis hj18-4 from traditional fermented soybean food inhibits bacillus cereus growth and toxin-related genes. | bacillus subtilis hj18-4 isolated from buckwheat sokseongjang, a traditional korean fermented soybean food, exhibits broad-spectrum antimicrobial activity against foodborne pathogens, including bacillus cereus. in this study, we investigated the antibacterial efficacy and regulation of toxin gene expression in b. cereus by b. subtilis hj18-4. expression of b. cereus toxin-related genes (groel, nhea, nhec, and entfm) was downregulated by b. subtilis hj18-4, which also exhibited strong antibacteri ... | 2014 | 25359543 |
| the biosynthesis of udp-d-fucnac-4n-(2)-oxoglutarate (udp-yelosamine) in bacillus cereus atcc 14579: pat and pyl, an aminotransferase and an atp-dependent grasp protein that ligates 2-oxoglutarate to udp-4-amino-sugars. | surface glycan switching is often observed when micro-organisms transition between different biotic and abiotic niches, including biofilms, although the advantages of this switching to the organism are not well understood. bacillus cereus grown in a biofilm-inducing medium has been shown to synthesize an unusual cell wall polysaccharide composed of the repeating subunit →6)gal(α1-2)(2-r-hydroxyglutar-5-ylamido)fuc2nac4n(α1-6)glcnac(β1→, where galactose is linked to the hydroxyglutarate moiety of ... | 2014 | 25368324 |
| infection of cardiovascular implantable electronic devices: detection with sonication, swab cultures, and blood cultures. | the number of patients with cardiovascular implantable electronic devices (cieds) is increasing. the purpose of this study was to compare three methods (sonication, swab cultures, and blood cultures) to detect bacteria in infections of cieds. | 2015 | 25377386 |
| evaluation of antibacterial and anthelmintic activities with total phenolic contents of piper betel leaves. | the study was conducted to investigate the antibacterial and anthelmintic activities and to determine total phenolic contents of methanolic extract of piper betel leaves. | 2014 | 25386394 |
| bacillus cereus ar156-induced resistance to colletotrichum acutatum is associated with priming of defense responses in loquat fruit. | the effectiveness of a biocontrol agent bacillus cereus ar156 for control of anthracnose rot caused by colletotrichum acutatum in harvested loquat fruit and the possible mechanisms of its action have been investigated. treatment of fruit with b. cereus ar156 resulted in lower disease incidence and smaller lesion diameters compared with that of untreated fruit. the treatment enhanced activities of defense-related enzymes including chitinase, β-1, 3-glucanase, phenylalanine ammonia-lyase, peroxida ... | 2014 | 25386680 |
| infrared decontamination of oregano: effects on bacillus cereus spores, water activity, color, and volatile compounds. | infrared (ir) heating, a novel technology for decontaminating oregano, was evaluated by investigating the reduction of inoculated bacillus cereus spores and the effect on water activity (a(w)), color, and headspace volatile compounds after exposure to ir treatment. conditioned oregano (a(w) 0.88) was ir-treated in a closed heating unit at 90 and 100 °c for holding times of 2 and 10 min, respectively. the most successful reduction in b. cereus spore numbers (5.6 log units) was achieved after a ho ... | 2014 | 25393824 |
| comparison of 3 selective media for enumeration of bacillus cereus in several food matrixes. | in this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin b agar [mypa], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [pemba], and brillance bacillus cereus agar [bbc agar]) for bacillus cereus (b. cereus) from pure culture and several food samples. bbc agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media sh ... | 2014 | 25399752 |
| kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms. | kawasaki disease (kd) is a systemic vasculitis of unknown etiology. the innate immune system is involved in its pathophysiology at the acute phase. we have recently established a novel murine model of kd coronary arteritis by oral administration of a synthetic microbe-associated molecular pattern (mamp). on the hypothesis that specific mamps exist in kd sera, we have searched them to identify kd-specific molecules and to assess the pathogenesis. | 2014 | 25411968 |
| screening of microorganisms from deep-sea mud for antarctic krill (euphausia superba) fermentation and evaluation of the bioactive compounds. | twelve kinds of strains were isolated from deep-sea mud which can use antarctic krill powder as the sole carbon/nitrogen source. these strains were identified by 16s rdna sequence analysis and grouped into eight different genera, including bacillus, shewanella, psychrobacter, klebsiella, macrococcus, aeromonas, acinetobacter, and saccharomyces. after fermentation of antarctic krill powder using these strains, bioactive compounds including total phenolics, free amino acids, and enzyme activities ... | 2015 | 25416479 |
| in vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (solanum tuberosum cv vitelotte noire) following simulated gastro-intestinal digestion. | analyses of antioxidant and in vitro antimicrobial and anti-proliferative activities of anthocyanin-rich extracts from purple potatoes, solanum tuberosum l. cv vitelotte noire (solanaceae), were performed by simulating both a domestic cooking process and human digestion. extracts of crude and cooked purple potato did not exhibit antimicrobial activity against the tester strains: bacillus cereus, escherichia coli and pseudomonas aeruginosa. the behaviour changed after the simulated gastrointestin ... | 2015 | 25420792 |
| cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in bacillus cereus atcc 14579. | loss of the cytochrome c maturation system in bacillus cereus results in increased transcription of the major enterotoxin genes nhe, hbl, and cytk and the virulence regulator plcr. increased virulence factor production occurs at 37°c under aerobic conditions, similar to previous findings in bacillus anthracis. unlike b. anthracis, much of the increased virulence gene expression can be attributed to loss of only c551, one of the two small c-type cytochromes. additional virulence factor expression ... | 2015 | 25422307 |
| production and purification of anti-bacterial biometabolite from wild-type lactobacillus, isolated from fermented bamboo shoot: future suggestions and a proposed system for secondary metabolite onsite recovery during continuous fermentation. | wild-type lactobacillus isolated form khorisa, a fermented bamboo shoot product of assam, india were evaluated for production anti-bacterial secondary biometabolites, against staphylococcus aureus. submerged fermentation technique was used for the production of secondary anti-microbial biometabolite by a single wild-type lactobacillus strain, which tested positive for the release of anti-bacterial factor(s). crude cell-free supernatant was obtained, followed by extraction in water-immiscible sol ... | 2015 | 25432345 |
| pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea. | linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea. | 2015 | 25433250 |
| [purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains]. | recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight. | 2014 | 25434178 |
| antimicrobial and anti-inflammatory activities of pleurostylia capensis turcz (loes) (celastraceae). | pleurostylia capensis is a large tree that can reach the maximum height of 20 m long, and it have been traditionally used as cosmetic, for steam bath, ritual body wash, and as a purgative to treat symptoms of witchcraft. using ethanol, chloroform, dichloromethane (dcm), ethyl acetate (ea), and water extracts, leaves, bark and roots of pleurostylia capensis were investigated scientifically for their effectiveness in antimicrobial, antioxidant and anti-inflammatory activities using standard method ... | 2014 | 25435632 |
| probabilistic exposure assessment model to estimate aseptic-uht product failure rate. | aseptic-ultra-high-temperature (uht) products are manufactured to be free of microorganisms capable of growing in the food at normal non-refrigerated conditions at which the food is likely to be held during manufacture, distribution and storage. two important phases within the process are widely recognised as critical in controlling microbial contamination: the sterilisation steps and the following aseptic steps. of the microbial hazards, the pathogen spore formers clostridium botulinum and baci ... | 2015 | 25440556 |
| antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain. | the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ... | 2015 | 25440998 |
| phospholipase c from pseudomonas aeruginosa and bacillus cereus; characterization of catalytic activity. | to study characteristics of phospholipases c (plcs), their importance for producing microorganisms as well as the potential of their use for industrial purposes. | 2014 | 25441984 |