Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| gastrointestinal parasitosis: histopathological insights to rare but intriguing lesions of the gastrointestinal tract. | gastrointestinal parasitosis is a significant cause of morbidity and mortality. definitive diagnosis is usually made by stool tests and/or serology but may require tissue evaluation. although pathologists are usually familiar with common parasites, it is not well established whether the diagnosis could be suspected without seeing the "parasite" itself. | 2016 | 27136106 |
| entamoeba histolytica induces human neutrophils to form nets. | entamoeba histolytica invades the intestine and other organs during the pathogenesis of amoebiasis. in the early stages, the host organism responds with an inflammatory infiltrate composed mostly of neutrophils. it has been reported that these immune cells, activated by e. histolytica, exert a protective role by releasing proteolytic enzymes and generating reactive oxygen/nitrogen species (ros/rns) and antimicrobial peptides. it is now known that neutrophils also produce neutrophil extracellular ... | 2016 | 27138813 |
| species-specific immunodetection of an entamoeba histolytica cyst wall protein. | entamoeba histolytica causes intestinal disease in endemic settings throughout the world. diagnosis of e. histolytica infection would be improved by the identification of biomarkers that are expressed by cysts of e. histolytica, but not by cysts of closely related commensal species of entamoeba. herein, we describe two novel monoclonal antibodies (1a4 and 1d3) produced against a spacer region of the e. histolytica jacob2 lectin, an outer cyst wall protein. these reagents demonstrated no cross-re ... | 2016 | 27152855 |
| detection of beta-tubulin in the cytoplasm of the interphasic entamoeba histolytica trophozoites. | it is known that the microtubules (mt) of entamoeba histolytica trophozoites form an intranuclear mitotic spindle. however, electron microscopy studies and the employment of anti-beta-tubulin (β-tubulin) antibodies have not exhibited these cytoskeletal structures in the cytoplasm of these parasites. the purpose of this work was to detect β-tubulin in the cytoplasm of interphasic e. histolytica trophozoites. activated or non-activated hmi-imss-strain e. histolytica trophozoites were used and cult ... | 2016 | 27156446 |
| cryptosporidium and other intestinal parasitic infections among hiv patients in southern ethiopia: significance of improved hiv-related care. | intestinal parasitic infections are known to cause gastroenteritis, leading to higher morbidity and mortality, particularly in people living with hiv/aids. this study aimed to determine the prevalence of cryptosporidium and other intestinal parasitic infections among hiv patients receiving care at a hospital in ethiopia where previous available baseline data helps assess if improved hiv-related care has reduced infection rates. | 2016 | 27165271 |
| amoebiasis vaccine development: a snapshot on e. histolytica with emphasis on perspectives of gal/galnac lectin. | amoebiasis/amebiasis is a gastrointestinal infection caused by an enteric dwelling protozoan, entamoeba histolytica. the disease is endemic in the developing world and is transmitted mainly via the faecal-oral route (e.g., in water or food) and may or may not be symptomatic. this disease of socio-economic importance worldwide involves parasite adherence and cytolysis of human cells followed by invasion that is mediated by galactose-binding (gal/galnac) surface lectin. disruption of the mucus lay ... | 2016 | 27181579 |
| diagnostic performances of antigen detection compared to conventional and nucleic acid detection of entamoeba histolytica in a non-endemic setting. | this study evaluated the immunochromatographic (ic) assay "techlab(®) e. histolytica quik chek™" analysing 36 faecal samples and 7 cultured strains. this assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. the ic assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect e. histolytica antigen in frozen faeces. in our laboratory located in a non-endemic setting this assay could not rep ... | 2016 | 27196557 |
| proteomic study of entamoeba histolytica trophozoites, cysts, and cyst-like structures. | the cyst stage of entamoeba histolytica is a promising therapeutic target against human amoebiasis. our research team previously reported the production in vitro of cyst-like structures (cls) sharing structural features with cysts, including rounded shape, size reduction, multinucleation, and the formation of a chitin wall coupled to the overexpression of glucosamine 6-phosphate isomerase, the rate-limiting enzyme of the chitin synthesis pathway. a proteomic study of e. histolytica trophozoites, ... | 2016 | 27228164 |
| an entamoeba histolytica adp-ribosyl transferase from the diphtheria toxin family modifies the bacterial elongation factor tu. | adp-ribosyl transferases are enzymes involved in the post-translational modification of proteins; they participate in multiple physiological processes, pathogenesis and host-pathogen interactions. several reports have characterized the functions of these enzymes in viruses, prokaryotes and higher eukaryotes, but few studies have reported adp-ribosyl transferases in lower eukaryotes, such as parasites. the locus ehi_155600 from entamoeba histolytica encodes a hypothetical protein that possesses a ... | 2016 | 27234208 |
| nitroimidazole carboxamides as antiparasitic agents targeting giardia lamblia, entamoeba histolytica and trichomonas vaginalis. | diarrhoeal diseases caused by the intestinal parasites giardia lamblia and entamoeba histolytica constitute a major global health burden. nitroimidazoles are first-line drugs for the treatment of giardiasis and amebiasis, with metronidazole 1 being the most commonly used drug worldwide. however, treatment failures in giardiasis occur in up to 20% of cases and development of resistance to metronidazole is of concern. we have re-examined 'old' nitroimidazoles as a foundation for the systematic dev ... | 2016 | 27236016 |
| horizontal gene transfers from bacteria to entamoeba complex: a strategy for dating events along species divergence. | horizontal gene transfer has proved to be relevant in eukaryotic evolution, as it has been found more often than expected and related to adaptation to certain niches. a relatively large list of laterally transferred genes has been proposed and evaluated for the parasite entamoeba histolytica. the goals of this work were to elucidate the importance of lateral gene transfer along the evolutionary history of some members of the genus entamoeba, through identifying donor groups and estimating the di ... | 2016 | 27239333 |
| evaluation of five commercial methods for the extraction and purification of dna from human faecal samples for downstream molecular detection of the enteric protozoan parasites cryptosporidium spp., giardia duodenalis, and entamoeba spp. | high quality, pure dna is required for ensuring reliable and reproducible results in molecular diagnosis applications. a number of in-house and commercial methods are available for the extraction and purification of genomic dna from faecal material, each one offering a specific combination of performance, cost-effectiveness, and easiness of use that should be conveniently evaluated in function of the pathogen of interest. in this comparative study the marketed kits qiaamp dna stool mini (qiagen) ... | 2016 | 27241828 |
| immune response of amebiasis and immune evasion by entamoeba histolytica. | entamoeba histolytica is a protozoan parasite and the causative agent of amebiasis. it is estimated approximately 1% of humans are infected with e. histolytica, resulting in an estimate of 100,000 deaths annually. clinical manifestations of amebic infection range widely from asymptomatic to severe symptoms, including dysentery and extra-intestinal abscesses. like other infectious diseases, it is assumed that only ~20% of infected individuals develop symptoms, and genetic factors of both the para ... | 2016 | 27242782 |
| heterogeneity of the serine synthetic pathway in entamoeba species. | phosphoserine phosphatase (psp) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. in the present study, we examined the existence of psp among genus entamoeba including a human enteric parasite, entamoeba histolytica. e. histolytica as well as majority of entamoeba species have the first and second enzymes, but lacks psp. in contra ... | 2016 | 27268730 |
| serum stress responsive gene ehslncrna of entamoeba histolytica is a novel long noncoding rna. | non coding rnas are known to play important roles in regulating gene expression at the transcriptional and posttranscriptional levels in metazoans. there is very little information available about non coding rnas in protists such as entamoeba histolytica. antisense and micro rnas have been reported in e. histolytica, however no long non coding rnas has been reported yet. here, we report our findings on an in vitro serum stress-inducible gene ehslncrna, a member of b1 transmembrane kinase family ... | 2016 | 27273618 |
| neglected waterborne parasitic protozoa and their detection in water. | outbreak incidents raise the question of whether the less frequent aetiological agents of outbreaks are really less frequent in water. alternatively, waterborne transmission could be relevant, but the lack of attention and rapid, sensitive methods to recover and detect the exogenous stages in water may keep them under-recognized. high quality information on the prevalence and detection of less frequent waterborne protozoa, such as cyclospora cayetanensis, toxoplasma gondii, isospora belli, balan ... | 2016 | 27281375 |
| increases in entamoeba histolytica antibody-positive rates in human immunodeficiency virus-infected and noninfected patients in japan: a 10-year hospital-based study of 3,514 patients. | serological evidence of the epidemiological trends in entamoeba histolytica infection is scarce, especially in nonendemic countries. we aimed to determine the antibody-positive rates over a 10-year period, and compare the trends between human immunodeficiency virus (hiv)-infected and -noninfected patients. we reviewed 3,514 patients who underwent antibody testing during the study periods, which were divided into five annual categories: 2004-2005, 2006-2007, 2008-2009, 2010-2011, and 2012-2013. a ... | 2016 | 27296390 |
| tyrosine phosphorylation/dephosphorylation of myosin ii essential light chains of entamoeba histolytica trophozoites regulates their motility. | entamoeba histolytica trophozoites dwell in the human intestine as comensals although under still unclear circumstances become invasive and destroy the host tissues. for these activities, trophozoites relay on remarkable motility provided by the cytoskeleton organization. amebic actin and some of its actin-associated proteins are well known, while components of the myosin ii molecule, although predicted from the e. histolytica genome, need biochemical and functional characterization. recently, a ... | 2016 | 27318258 |
| regioselective phosphorylation of myo-inositol with binol-derived phosphoramidites and its application for protozoan lysophosphatidylinositol. | a regioselective phosphorylation method for myo-inositol was developed by utilizing readily preparable binol-derived phosphoramidites. the method also facilitated the complete separation of the diastereomeric products by simple chromatography. based on this phosphorylation and ni-catalyzed alkyl-alkyl cross-coupling reaction for long fatty acids, we achieved the first synthesis of a lysophosphatidylinositol, ehpia having long fatty acid c30:1, as a partial structure of glycosylphosphatidylinosit ... | 2016 | 27326923 |
| a retrospective analysis of the results of a five-year (2005-2009) parasitological examination for common intestinal parasites from bale-robe health center, robe town, southeastern ethiopia. | a cross-sectional retrospective survey using the past five years clinical records (2005-2009) was conducted. the study was aimed at assessing the status of common intestinal parasites from bale-robe health center, southeastern ethiopia, in 2009/2010. the survey involved collection of data recorded on intestinal parasite from the health center during 2005-2009. precoded questionnaires and interviews were also supplemented for knowledge attitude practices survey (kaps survey) to assess awareness l ... | 2013 | 27335857 |
| cross-sectional study on the prevalence of intestinal parasites and associated risk factors in teda health centre, northwest ethiopia. | objective. to assess the magnitude of intestinal parasitic infection and associated risk factors in teda health centre, northwest ethiopia. method. a cross-sectional study was conducted in teda health centre from february to april, 2011. stool samples were collected from 410 study participants and analysed by direct wet mount and formal ether concentration techniques. furthermore, sociodemographic data were collected by using standardized questionnaire. result. the overall prevalence of intestin ... | 2013 | 27335860 |
| accuracy of mobile phone and handheld light microscopy for the diagnosis of schistosomiasis and intestinal protozoa infections in côte d'ivoire. | handheld light microscopy using compact optics and mobile phones may improve the quality of health care in resource-constrained settings by enabling access to prompt and accurate diagnosis. | 2016 | 27348755 |
| high occurrence of blastocystis sp. subtypes 1-3 and giardia intestinalis assemblage b among patients in zanzibar, tanzania. | blastocystis is a common intestinal parasite with worldwide distribution but the distribution of blastocystis and its subtypes in east africa is largely unknown. in this study, we investigate the distribution of blastocystis subtypes in zanzibar, tanzania and report the prevalence of intestinal parasites using both molecular methods and microscopy. | 2016 | 27356981 |
| prevalence of entamoeba histolytica, giardia lamblia, and cryptosporidium spp. in libya: 2000-2015. | the intestinal protozoa entamoeba histolytica, giardia lamblia, and cryptosporidium spp. are the causative agents of giardiasis, amebiasis, and cryptosporidiosis, respectively. adequate knowledge of the geographical distribution of parasites and the demographic variables that influence their prevalence is important for effective control of infection in at-risk populations. | 2016 | 27363524 |
| molecular detection of common intestinal parasites: a performance evaluation of the bd max™ enteric parasite panel. | the purpose of this study was to evaluate the level of agreement of the bd max™ enteric parasite panel (epp) with microscopy for the detection of giardia duodenalis, cryptosporidium spp. and entamoeba histolytica in stool samples. a total of 372 stool samples (partly collected on the basis of positive microscopy and partly unselected, consecutive sample submitted for parasite investigation) were tested with epp according to manufacturer's instructions and also using microscopy according to stand ... | 2016 | 27394158 |
| irritable bowel syndrome and intestinal parasites: a view from south america. | irritable bowel syndrome (ibs) is a functional gastrointestinal disorder of uncertain etiology. several studies have proposed the possible role of intestinal parasites in the pathogenesis of ibs. we aimed to summarize the epidemiological studies that describe a possible link between intestinal parasites and ibs, with special interest in endemic areas for intestinal parasitism such as south america. a comprehensive review of the literature was conducted by using the keywords: irritable bowel synd ... | 2016 | 27409092 |
| entamoeba histolytica trophozoites and lipopeptidophosphoglycan trigger human neutrophil extracellular traps. | neutrophil defense mechanisms include phagocytosis, degranulation and the formation of extracellular traps (net). these networks of dna are triggered by several immune and microbial factors, representing a defense strategy to prevent microbial spread by trapping/killing pathogens. this may be important against entamoeba histolytica, since its large size hinders its phagocytosis. the aim of this study was to determine whether e. histolytica and their lipopeptidophosphoglycan (ehlppg) induce the f ... | 2016 | 27415627 |
| prevalence of intestinal protozoans among schoolchildren in suburban areas near yangon, myanmar. | although intestinal protozoans are common etiologies of diarrhea, few studies have been conducted in myanmar. this study planned to investigate the prevalence of giardia lamblia, entamoeba coli, entamoeba histolytica, and endolimax nana among schoolchildren and their guardians in suburban areas near yangon, myanmar. we performed a cross-sectional survey among schoolchildren and their guardians from 7 primary schools in south dagon and hlaing thar yar districts, yangon, myanmar. stool samples wer ... | 2016 | 27417092 |
| simultaneous detection and differentiation of entamoeba histolytica, e. dispar, e. moshkovskii, giardia lamblia and cryptosporidium spp. in human fecal samples using multiplex pcr and qpcr-mca. | entamoeba histolytica, giardia lamblia and cryptosporidium spp. are common causes of diarrheal and intestinal diseases all over the world. microscopic methods are useful in the diagnosis of intestinal parasites (ips), but their sensitivity was assessed approximately 60 percent. recently, molecular techniques have been used increasingly for the identification and characterization of the parasites. among those, in this study we have used multiplex pcr and real-time pcr with melting curve analysis ... | 2016 | 27423396 |
| antiamoebic activity of adenophyllum aurantium (l.) strother and its effect on the actin cytoskeleton of entamoeba histolytica. | in mexico, the adenophyllum aurantium (l.) strother plant is consumed as an infusion to treat intestinal diseases such as amoebiasis, which is an endemic health problem in mexico and other countries. however, the effect of a. aurantium on entamoeba histolytica, the causative agent of amoebiasis, is unknown. an aerial part methanolic extract (aamea), a root methanolic extract (aamer) and a root ethyl acetate extract (aaear) were tested on e. histolytica trophozoites. aamea and aamer did not show ... | 2016 | 27445810 |
| evaluation of the nanochip® gastrointestinal panel (gip) test for simultaneous detection of parasitic and bacterial enteric pathogens in fecal specimens. | infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. current laboratory stool testing has a long turnaround time (tat) and demands highly qualified personnel and multiple techniques. the need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising pe ... | 2016 | 27447173 |
| role of serum amyloid a, granulocyte-macrophage colony-stimulating factor, and bone marrow granulocyte-monocyte precursor expansion in segmented filamentous bacterium-mediated protection from entamoeba histolytica. | intestinal segmented filamentous bacteria (sfb) protect from ameba infection, and protection is transferable with bone marrow dendritic cells (bmdcs). sfb cause an increase in serum amyloid a (saa), suggesting that saa might mediate sfb's effects on bmdcs. here we further explored the role of bone marrow in sfb-mediated protection. transient gut colonization with sfb or saa administration alone transiently increased the h3k27 histone demethylase jmjd3, persistently increased bone marrow csf2ra e ... | 2016 | 27456830 |
| modulation of innate immune response by the vagus nerve in experimental hepatic amebiasis in rats. | the parasympathetic nervous system has a crucial role in immunomodulation of the vagus nerve, its structure provides a pathogen detection system, and a negative feedback to the immune system after the pathogenic agent has been eliminated. amebiasis is a disease caused by the protozoan parasite entamoeba histolytica, considered the third leading cause of death in the world. the rats are used as a natural resistance model to amoebic liver infection. the aim of this study is to analyze the interact ... | 2016 | 27466057 |
| fulminant amebic colitis after corticosteroid therapy: a systematic review. | amebic colitis, caused by intestinal infection with the parasite, entamoeba histolytica, is a common cause of diarrhea worldwide. fulminant amebic colitis is the most devastating complication of this infection, associated with both high mortality and morbidity. we conducted a review of the english literature to describe cases of fulminant amebic colitis associated with exposure to corticosteroid medications in order to identify the risk factors for poor outcome and determine difficulties in diag ... | 2016 | 27467600 |
| primary pulmonary amebiasis complicated with multicystic empyema. | amebiasis is a parasitic infection caused by the protozoan entamoeba histolytica. while most infections are asymptomatic, the disease could manifest clinically as amebic dysentery and/or extraintestinal invasion in the form of amebic liver abscess or other more rare manifestations such as pulmonary, cardiac, or brain involvement. herein we are reporting a case of a 24-year-old male with history of down syndrome who presented with severe right side pneumonia complicated with multicystic empyema r ... | 2016 | 27478673 |
| role of cysteine residues in the redox-regulated oligomerization and nucleotide binding to ehrabx3. | the enteric protozoan parasite, entamoeba histolytica, an etiological agent of amebiasis, is involved in the adhesion and destruction of human tissues. worldwide, the parasite causes about 50 million cases of amebiasis and 100,000 deaths annually. ehrabx3, a unique amoebic rab gtpase with tandem g-domains, possesses an unusually large number of cysteine residues in its n-terminal domain. crystal structure of ehrabx3 revealed an intra-molecular disulfide bond between c39 and c163 which is critica ... | 2016 | 27485554 |
| a decade of intestinal protozoan epidemiology among settled immigrants in qatar. | the world health organization estimates that about 3.5 billion people worldwide are affected by intestinal parasitic infections. reports have already emphasized the role of immigrants in outbreaks of parasitic diseases in industrialized countries. with the mass influx of immigrants to qatar, patent intestinal parasitic infections have been observed. herein, the prevalence of intestinal protozoan infections was analysed in 29,286 records of subjects referred for stool examination at the hamad med ... | 2016 | 27496143 |
| the motility of entamoeba histolytica: finding ways to understand intestinal amoebiasis. | the pathogenic amoeba entamoeba histolytica is able to migrate within various compartments of the human body. the present article reviews progress in understanding the mechanisms of cell motility in e. histolytica during human intestinal invasion and, in particular, how the three-dimensional characteristics of the environment regulate the parasite's behaviour. the amoeboid mode of migration that applies to e. histolytica's displacements on two-dimensional surfaces is also expected to apply to th ... | 2016 | 27497052 |
| the intestinal bacterial microbiome and e. histolytica infection. | entamoeba histolytica, the etiological agent of amebiasis, is a significant cause of pediatric diarrhea in south asia and sub-saharan africa. the clinical outcome of an e. histolytica exposure varies enormously and can present as diarrhea, dysentery, or amebic liver abscess. host and parasite factors likely contribute to the outcome of infection with the parasite, but do not explain the wide variation in presentation of disease. this suggests that other environmental factors affect disease. an e ... | 2017 | 27525214 |
| multicenter evaluation of bd max enteric parasite real-time pcr assay for detection of giardia duodenalis, cryptosporidium hominis, cryptosporidium parvum, and entamoeba histolytica. | common causes of chronic diarrhea among travelers worldwide include protozoan parasites. the majority of parasitic infections are caused by giardia duodenalis, entamoeba histolytica, cryptosporidium parvum, and cryptosporidium hominis similarly, these species cause the majority of parasitic diarrhea acquired in the united states. detection of parasites by gold standard microscopic methods is time-consuming and requires considerable expertise; enzyme immunoassays and direct fluorescent-antibody ( ... | 2016 | 27535690 |
| comparison of various methods in the diagnosis of entamoeba histolytica in stool and serum specimens. | entamoeba histolytica is indistinguishable from entamoeba dispar in direct microscopic examination. a definitive diagnosis of e. histolytica is important in terms of the treatment of the patient and to avoid unnecessary costs. this study's aim is to determine the prevalence of e. histolytica and to make a comparison of the different diagnostic tests in the patients specimens defined as e. histolytica/e. dispar infection. | 2016 | 27551176 |
| overexpression of differentially expressed genes identified in non-pathogenic and pathogenic entamoeba histolytica clones allow identification of new pathogenicity factors involved in amoebic liver abscess formation. | we here compared pathogenic (p) and non-pathogenic (np) isolates of entamoeba histolytica to identify molecules involved in the ability of this parasite to induce amoebic liver abscess (ala)-like lesions in two rodent models for the disease. we performed a comprehensive analysis of 12 clones (a1-a12) derived from a non-pathogenic isolate hm-1:imss-a and 12 clones (b1-b12) derived from a pathogenic isolate hm-1:imss-b. "non-pathogenicity" included the induction of small and quickly resolved lesio ... | 2016 | 27575775 |
| performance of microscopy and elisa for diagnosing giardia duodenalis infection in different pediatric groups. | techniques for giardia diagnosis based on microscopy are usually applied as routine laboratory testing; however, they typically exhibit low sensitivity. this study aimed to evaluate giardia duodenalis and other intestinal parasitic infections in different pediatric groups, with an emphasis on the comparison of giardia diagnostic techniques. feces from 824 children from different groups (diarrheic, malnourished, with cancer and from day care) were examined by microscopy and elisa for giardia, cry ... | 2016 | 27586394 |
| synthesis, antiamoebic and molecular docking studies of furan-thiazolidinone hybrids. | in continuation of our previous work, a series of furan-thiazolidinone hybrids was prepared by knoevenagel condensation of 3-(furan-2-ylmethyl)-2-(phenylimino)-1, 3-thiazolidin-4-one with different aryl aldehydes in presence of strong base. some members of the series exhibited remarkable antiamoebic activity and cell viability. three compounds (3, 6 and 11) showed excellent binding energy for entamoeba histolytica o-acetyle-l-serine sulfohydrolase and entamoeba histolytica thioredoxin reductase. ... | 2016 | 27597415 |
| enteric pathogens and factors associated with acute bloody diarrhoea, kenya. | shigellosis is the major cause of bloody diarrhoea worldwide and is endemic in most developing countries. in kenya, bloody diarrhoea is reported weekly as part of priority diseases under integrated disease surveillance and response system (idsr) in the ministry of health. | 2016 | 27600526 |
| comparison of microscopic and immunoassay examination in the diagnosis of intestinal protozoa of humans in mansoura, egypt. | protozoal diseases are prevalent globally and especially in developing countries that have relatively lower socioeconomic populations such as egypt. direct microscopic examination (dme) is used for the detection and identification of protozoa but lacks sufficient reliability, and thus may be detrimental in obtaining accurate diagnostic or epidemiological data. in this study, we determine the prevalence of infections by giardia intestinalis, cryptosporidium sp., and entamoeba histolytica in human ... | 2016 | 27605751 |
| entamoeba histolytica: overexpression of the gal/galnac lectin, ehcp2 and ehcp5 genes in an in vivo model of amebiasis. | the parasite entamoeba histolytica causes intestinal amebiasis and amebic liver abscess as its main extraintestinal manifestation. to study the in vivo events related to inflammation and the interactions between hosts and parasites during amebiasis, we designed a novel model of host-parasite interactions using cellulose membrane dialysis bags containing e. histolytica trophozoites. a bag is placed into the hamster peritoneal cavity, as has been reported in previous studies of programmed cell dea ... | 2016 | 27616150 |
| entamoeba histolytica and entamoeba dispar infection in mexican school children: genotyping and phylogenetic relationship. | this study aimed to determine the frequency of entamoeba histolytica and entamoeba dispar infection in school children in the community of tlaltizapan, in order to understand the dynamics of infection within the school and family spheres of this population. amoebiasis is an unsolved public health problem and an endemic disease in mexico. the incidence rate varies depending on the state; the most affected states show the highest numbers of new cases of amoebiasis per year. previously, we reported ... | 2016 | 27618874 |
| prevalence of amebiasis in inflammatory bowel disease in university clinical hospital mostar. | to explore the prevalence of amebiasis in inflammatory bowel disease (ibd), crohn's disease and ulcerative colitis, in patients in clinical hospital mostar (bosnia and herzegovina, region of herzegovina). | 2016 | 27652159 |
| role of ehrab7a in phagocytosis of type 1 fimbriated e. coli by entamoeba histolytica. | entamoeba histolytica, the causative agent of amoebic colitis and liver abscess in human, ingests the intestinal bacteria and variety of host cells. phagocytosis of bacteria by the amebic trophozoite has been reported to be important for the virulence of the parasite. here, we set out to characterize different stages of phagocytosis of type 1 e. coli and investigated the role of a set of amoebic rab gtpases in the process. the localizations of the rab gtpases during different stages of the phago ... | 2016 | 27663892 |
| characterization of the recombination activities of the entamoeba histolytica rad51 recombinase. | the protozoan parasite responsible for human amoebiasis is entamoeba histolytica. an important facet of the life cycle of e. histolytica involves the conversion of the mature trophozoite to a cyst. this transition is thought to involve homologous recombination (hr), which is dependent upon the rad51 recombinase. here, a biochemical characterization of highly purified ehrad51 protein is presented. the ehrad51 protein preferentially binds ssdna, forms a presynaptic filament and possesses atp hydro ... | 2017 | 27678398 |
| intestinal parasitic infections and associated epidemiological drivers in two rural communities of the bolivian chaco. | in 2013 a coproparasitological survey was carried out in two rural communities of the bolivian chaco to determine the prevalence of intestinal parasitic infections (ipis) and to investigate on possible infection drivers through a questionnaire interview. | 2016 | 27694735 |
| recent progress on the discovery of antiamoebic agents. | a large number of protozoans infect humans but entamoeba histolytica is the only organism responsible for causing amoebiasis, a deadly disease after malaria. numerous heterocycle-based antiamoebic agents have been previously synthesized as e. histolytica inhibitors and while some of these agents have shown moderate activity, the search for a novel and ideal antiamoebic compound is still ongoing. in this digest letter, we present the latest data on antiamoebic agents from 2011 to 2016 based on th ... | 2016 | 27707603 |
| the potential of lactobacillus casei and entercoccus faecium combination as a preventive probiotic against entamoeba. | travellers' diarrhoea caused by enteric protozoa like entamoeba histolytica is among the most common protozoan diseases in developing countries. in developing countries, amoebiasis is the second most prevalent protozoan disease. this protozoan parasite is often known to coexist as a part of the normal gut microbiota. it is estimated that around 50-60 % of population in developing countries might be harbouring entamoeba in an asymptomatic manner. due to physiological perturbation or upon immuno-c ... | 2016 | 27709564 |
| prevalence of intestinal parasitic infections and associated risk factors among pregnant women attending antenatal care center at felege hiwot referral hospital, northwest ethiopia. | parasitic infections affect tens of millions of pregnant women worldwide, and directly or indirectly lead to a spectrum of adverse maternal and fetal/placental effects. the objective of this study was to assess the prevalence of intestinal parasite infections and associated risk factors among pregnant women attending antenatal care center in felege hiwot referral hospital, bahir dar city, northwest ethiopia. | 2016 | 27716099 |
| prevalence of amoebiasis in a model research community and its confirmation using stool antigen elisa for entamoeba histolytica. | entamoeba histolytica (e. histolytica) produces an invasive disease called amoebiasis, which commonly produces diarrhea with or without blood in both children and adults, leading to high morbidity and mortality. entamoeba dispar (e. dispar) is a non invasive, non pathogenic organism. both entamoeba histolytica and entamoeba dispar look alike on microscopy and therefore cannot be differentiated unless checked on elisa, pcr or other specific method. to calculate the actual prevalence of pathogenic ... | 2016 | 27731816 |
| prevalence of intestinal parasitic infections and associated risk factors among schoolchildren in the plateau central and centre-ouest regions of burkina faso. | unsafe drinking water, unimproved sanitation and lack of hygiene pose health risks, particularly to children in low- and middle-income countries. this study aimed to assess the prevalence and risk factors of intestinal parasitic infections in school-aged children in two regions of burkina faso. | 2016 | 27756339 |
| recurrent amebic liver abscesses over a 16-year period: a case report. | amebic liver abscess is a rare disease in high-income countries. recurrence of amebic liver abscess is even rarer with only a few previous reports. here we present a patient who developed three subsequent amebic liver abscesses over a sixteen-year period. | 2016 | 27756400 |
| efficiency of direct microscopy of stool samples using an antigen-specific adhesin test for entamoeba histolytica. | e. histolytica is among the common causes of acute gastroenteritis. the pathogenic species e. histolytica and the nonpathogenic species e. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. in many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using native-lugol's iodine for pre-diagnosis. | 2016 | 27761283 |
| gene migration for re-emerging amebiasis in iran's northwest-iraq borders: a microevolutionary scale for reflecting epidemiological drift of entamoeba histolytica metapopulations. | in the microevolutionary scales of entamoeba isolates, the gene migration shows how entamoeba spp. has epidemiologically drifted among border countries. five hundred fecal samples were taken from patients suffering gastrointestinal disorders, abdominal pain, and diarrhea at saggez, northwest iran located within the border iraq country. following parasitological techniques, dna samples were extracted and amplified by polymerase chain reaction (pcr) of 18s rrna region to identify entamoeba infecti ... | 2017 | 27761718 |
| entamoeba encystation: new targets to prevent the transmission of amebiasis. | amebiasis is caused by entamoeba histolytica infection and can produce a broad range of clinical signs, from asymptomatic cases to patients with obvious symptoms. the current epidemiological and clinical statuses of amebiasis make it a serious public health problem worldwide. the entamoeba life cycle consists of the trophozoite, the causative agent for amebiasis, and the cyst, the form responsible for transmission. these two stages are connected by "encystation" and "excystation." hence, develop ... | 2016 | 27764256 |
| extensive transcriptome analysis correlates the plasticity of entamoeba histolytica pathogenesis to rapid phenotype changes depending on the environment. | amoebiasis is a human infectious disease due to the amoeba parasite entamoeba histolytica. the disease appears in only 20% of the infections. diversity in phenotypes may occur within the same infectious strain in the gut; for instance, parasites can be commensal (in the intestinal lumen) or pathogenic (inside the tissue). the degree of pathogenesis of clinical isolates varies greatly. these findings raise the hypothesis that genetic derivation may account for amoebic diverse phenotypes. the main ... | 2016 | 27767091 |
| cardioprotective effects of monocyte locomotion inhibitory factor on myocardial ischemic injury by targeting vimentin. | monocyte locomotion inhibitory factor (mlif), a heat-stable pentapeptide produced by entamoeba histolytica, has anti-inflammatory function and protective effect on ischemic stroke. in this study, we evaluated the effect of mlif on myocardial ischemia. mice were subjected to ischemia/reperfusion by occlusion of the left anterior descending artery (lad). after sacrifice, the serum concentrations of cardiac troponin i (ctni), creatine kinase (ck), lactate dehydrogenase (ldh) as well as the heart in ... | 2016 | 27773717 |
| seroprevalence of five parasitic pathogens in pregnant women in ten caribbean countries. | to date, published epidemiological studies of parasitic infections in humans in the caribbean region are very limited. here, we report the seroprevalence of five parasitic pathogens, including ascaris lumbricoides, entamoeba histolytica, giardia lamblia, schistosoma mansoni, and toxocara canis in 435 serum samples collected between 2008 and 2011 from pregnant women in ten caribbean islands. we tested the serum samples for igg antibodies against the five parasites by enzyme-linked immunosorbent a ... | 2017 | 27778108 |
| a study of parasitic and bacterial pathogens associated with diarrhea in hiv-positive patients. | diarrhea is a common complication of acquired immune deficiency syndrome (aids), occurring in almost 90% of aids patients in developing countries like india. the present study was aimed to determine the prevalence and microbiological profile of pathogens associated with diarrhea in human immunodeficiency virus (hiv) positive patients and their relation to cd4 counts. | 2016 | 27795876 |
| identification of entamoeba histolytica and e. dispar infection in maceió, alagoas state, northeast brazil. | a cross-sectional study was carried out to assess the prevalence of e. histolytica and e. dispar by examining stool samples obtained from 1,003 students of public schools in maceió, alagoas, brazil. | 2016 | 27801380 |
| migrant workers in malaysia: current implications of sociodemographic and environmental characteristics in the transmission of intestinal parasitic infections. | a cross-sectional study of intestinal parasitic infections amongst migrant workers in malaysia was conducted. a total of 388 workers were recruited from five sectors including manufacturing, construction, plantation, domestic and food services. the majority were recruited from indonesia (n = 167, 43.3%), followed by nepal (n = 81, 20.9%), bangladesh (n = 70, 18%), india (n = 47, 12.1%) and myanmar (n = 23, 5.9.2%). a total of four nematode species (ascaris lumbricoides, trichuris trichiura, ente ... | 2016 | 27806046 |
| n-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of entamoeba histolytica to nitrosative stress. | adaptation of the entamoeba histolytica parasite to toxic levels of nitric oxide (no) that are produced by phagocytes may be essential for the establishment of chronic amebiasis and the parasite's survival in its host. in order to obtain insight into the mechanism of e. histolytica's adaptation to no, e. histolytica trophozoites were progressively adapted to increasing concentrations of the no donor drug, s-nitrosoglutathione (gsno) up to a concentration of 110 μm. the transcriptome of no adapte ... | 2016 | 27808157 |
| phase i clinical trial results of auranofin, a novel antiparasitic agent. | under an nih priority to identify new drugs to treat class b parasitic agents, we performed high-throughput screens, which identified the activity of auranofin (ridaura) against entamoeba histolytica and giardia intestinalis, major causes of water- and foodborne outbreaks. auranofin, an orally administered, gold (au)-containing compound that was approved by the fda in 1985 for treatment of rheumatoid arthritis, was effective in vitro and in vivo against e. histolytica and both metronidazole-sens ... | 2017 | 27821451 |
| structures of parasite calreticulins provide insights into their flexibility and dual carbohydrate/peptide-binding properties. | calreticulin (crt) is a multifaceted protein, initially discovered as an endoplasmic reticulum (er) chaperone protein, that is essential in calcium metabolism. various implications in cancer, early development and immunology have been discovered more recently for crt, as well as its role as a dominant 'eat-me' prophagocytic signal. intriguingly, cell-surface exposure/secretion of crt is among the infective strategies used by parasites such as trypanosoma cruzi, entamoeba histolytica, taenia soli ... | 2016 | 27840680 |
| underestimated amoebic appendicitis among hiv-1-infected individuals in japan. | entamoeba histolytica is not a common causative agent of acute appendicitis. however, amoebic appendicitis can sometimes be severe and life threatening, mainly due to a lack of awareness. also, its frequency, clinical features, and pathogenesis remain unclear. the study subjects were hiv-1-infected individuals who presented with acute appendicitis and later underwent appendectomy at our hospital between 1996 and 2014. formalin-fixed paraffin-embedded preserved appendix specimens were reexamined ... | 2017 | 27847377 |
| recent advances in entamoeba biology: rna interference, drug discovery, and gut microbiome. | in recent years, substantial progress has been made in understanding the molecular and cell biology of the human parasite entamoeba histolytica, an important pathogen with significant global impact. this review outlines some recent advances in the entamoeba field in the last five years, focusing on areas that have not recently been discussed in detail: (i) molecular mechanisms regulating parasite gene expression, (ii) new efforts at drug discovery using high-throughput drug screens, and (iii) th ... | 2016 | 27853522 |
| the majority of cockroaches from the samutprakarn province of thailand are carriers of parasitic organisms. | we undertook a study of the mechanical transmission of parasitic organisms in cockroaches in the samutprakarn province of thailand. in this study, 920 cockroaches were obtained from 18 open-air shopping markets in 5 districts and 1 subdistrict of this province. all cockroaches were captured during their feeding time in their natural habitat. direct wet smear and modified acid-fast bacilli staining were used to identify the parasites from the external surface or cuticle of the cockroaches. the re ... | 2011 | 27857676 |
| multiplex real-time pcr assay targeting eight parasites customized to the korean population: potential use for detection in diarrheal stool samples from gastroenteritis patients. | intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. we developed a multiplex real-time pcr assay for detection of eight intestinal parasites and prospectively evaluated it for patients with gastroenteritis. the assay targeted cryptosporidium parvum, giardia lamblia, entamoeba histolytica, blastocystis hominis, dientamoeba fragilis, clonorchis sinensis, metagonimus yokogawai, an ... | 2016 | 27861635 |
| antioxidant defense of nrf2 vs pro-inflammatory system of nf-κb during the amoebic liver infection in hamster. | entamoeba histolytica is the causative agent of amoebic liver abscess (ala), which course with an uncontrolled inflammation and nitro-oxidative stresses, although it is well known that amoeba has an effective defence mechanisms against this toxic environment, the underlying molecular factors responsible for progression of tissue damage remain largely unknown. the purpose of the present study was to determine during the acute stage of ala in hamsters, the involvement of nuclear factor (erythroid- ... | 2017 | 27876112 |
| prevalence and risk factors of intestinal protozoan infections: a population-based study in rural areas of boyer-ahmad district, southwestern iran. | parasitic infections are still a significant health problem in rural areas in developing countries including iran. there is no recent population-based data about the prevalence of human intestinal parasites in most rural areas of iran. the current study aimed to determine the prevalence of intestinal protozoan infection in inhabitants of rural areas of boyer-ahmad district, southwestern iran. | 2016 | 27884121 |
| study of infections among human immunodeficiency virus/acquired immunodeficiency syndrome patients in shadan hospital, telangana, india. | human immunodeficiency virus (hiv) pandemicity is a major concern today as it causes greater loss of productivity than any other disease. hiv infection leads to profound immune deficiency and patients become highly susceptible to opportunistic infections (ois). hiv epidemic in india is heterogeneous in nature, both in terms of routes of transmission as well as geographical spread. | 2017 | 27890948 |
| functionally conserved rna-binding and protein-protein interaction properties of line-orf1p in an ancient clade of non-ltr retrotransposons of entamoeba histolytica. | retrotransposons are mobile genetic elements found in most organisms. their origin and evolution is not very well understood. retrotransposons that lack long terminal repeats (non-ltr) have been classified based on their reverse transcriptase (rt) and endonuclease sequences into groups, of which r2 is the most ancient. its members contain a single open reading frame (orf) while there are two orfs in the other groups, of which orf2 contains the rt and endonuclease sequences. it is thought that or ... | 2017 | 27894878 |
| proteome analysis of excretory-secretory proteins of entamoeba histolytica hm1:imss via lc-esi-ms/ms and lc-maldi-tof/tof. | excretory-secretory (es) proteins of e. histolytica are thought to play important roles in the host invasion, metabolism, and defence. elucidation of the types and functions of e. histolytica es proteins can further our understanding of the disease pathogenesis. thus, the aim of this study is to use proteomics approach to better understand the complex es proteins of the protozoa. | 2016 | 27895543 |
| lateral gene transfer of p-cresol- and indole-producing enzymes from environmental bacteria to mastigamoeba balamuthi. | p-cresol and indole are volatile biologically active products of the bacterial degradation of tyrosine and tryptophan respectively. they are typically produced by bacteria in animal intestines, soil and various sediments. here, we demonstrate that the free-living eukaryote mastigamoeba balamuthi and its pathogenic relative entamoeba histolytica produce significant amounts of indole via tryptophanase activity. unexpectedly, m. balamuthi also produces p-cresol in concentrations that are bacteriost ... | 2017 | 27902886 |
| distribution of parasites detected in stool samples of patients admitted to our parasitology laboratory during a three-year period between 2012 and 2014. | parasitic diseases are among the major public health issues worldwide. a number of tests are available for diagnosis, but the sentivity and specifity of these tests are assumed to be insufficient. nevertheless, the most common diagnostic method is microscopic examination. in this study, we aimed to introduce the distribution of parasites detected in stool samples of patients admitted to our laboratory on the basis of parameters such as, age, and gender during a 3-year period between 2012 and 201 ... | 2016 | 27905282 |
| improved anticancer and antiparasitic activity of new lawsone mannich bases. | substituted lawsone mannich bases 2a-e, 3a-e and 4a-e were prepared and tested for their biological activities. the new fatty alkyl substituted compounds 2a-c exhibited strong and selective growth inhibitory activities in the low one-digit micromolar and sub-micromolar range against a panel of human cancer cell lines associated with ros formation. in addition, compounds 2a-c revealed sub-micromolar anti-trypanosomal activities against parasitic trypanosoma brucei brucei cells via deformation of ... | 2017 | 27912173 |
| effect of mass stool examination and mass treatment for decreasing intestinal helminth and protozoan infection rates in bolivian children: a cross-sectional study. | bolivia is one of the countries with a high intestinal helminth and protozoan infection rate. despite the high prevalence of the parasitic infection, nationwide preventive measures for bolivian children have not yet been implemented. we evaluated the effect of mass stool examination and treatment as a strategy for decreasing the infection rate. this study was conducted between 2013 and 2015 in children aged 2-18 years. a total of 2,033 stool samples (575 in 2013, 815 in 2014 and 642 in 2015) wer ... | 2016 | 27923058 |
| metal dependence and branched rna cocrystal structures of the rna lariat debranching enzyme dbr1. | intron lariats are circular, branched rnas (brnas) produced during pre-mrna splicing. their unusual chemical and topological properties arise from branch-point nucleotides harboring vicinal 2',5'- and 3',5'-phosphodiester linkages. the 2',5'-bonds must be hydrolyzed by the rna debranching enzyme dbr1 before spliced introns can be degraded or processed into small nucleolar rna and microrna derived from intronic rna. here, we measure the activity of dbr1 from entamoeba histolytica by using a synth ... | 2016 | 27930312 |
| phosphorylation of eukaryotic initiation factor-2α during stress and encystation in entamoeba species. | entamoeba histolytica is an enteric pathogen responsible for amoebic dysentery and liver abscess. it alternates between the host-restricted trophozoite form and the infective environmentally-stable cyst stage. throughout its lifecycle e. histolytica experiences stress, in part, from host immune pressure. conversion to cysts is presumed to be a stress-response. in other systems, stress induces phosphorylation of a serine residue on eukaryotic translation initiation factor-2α (eif2α). this inhibit ... | 2016 | 27930733 |
| amoebic liver abscess: an unusual cause for a right iliac fossa mass: a case report. | amoebic liver abscess is the most common extra intestinal manifestation of amoebiasis in tropical countries. it usually presents with right hypochondrial pain, fever and anorexia. amoebic liver abscess has gained clinical significance due to the wide variety of clinical presentations which can cause diagnostic dilemmas and high mortality in untreated cases. | 2016 | 27931200 |
| the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
| (1)h, (13)c and (15)n nmr assignments of an unusual ca(2+)-binding protein from entamoeba histolytica in its apo form. | we report almost complete sequence specific (1)h, (13)c and (15)n nmr assignments of an unusual ca(2+)-binding protein from entamoeba histolytica (ehcabp6) in its apo form as a prelude to its structural and functional characterization. | 2017 | 27990612 |
| ehnpc1 and ehnpc2 proteins participate in trafficking of exogenous cholesterol in entamoeba histolytica trophozoites: relevance for phagocytosis. | entamoeba histolytica, the highly phagocytic protozoan causative of human amoebiasis lacks the machinery to synthesize cholesterol. here, we investigated the presence of npc1 and npc2 proteins in this parasite, which are involved in cholesterol trafficking in mammals. bioinformatics analysis revealed one ehnpc1 and two ehnpc2 genes. ehnpc1 appeared as a transmembrane protein and both ehnpc2 as peripheral membrane proteins. molecular docking predicted that ehnpc1 and ehnpc2 bind cholesterol and i ... | 2016 | 28002502 |
| diagnosis of intestinal parasites in a rural community of venezuela: advantages and disadvantages of using microscopy or rt-pcr. | a cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time pcr (rt-pcr) for 14 intestinal parasites in a venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. a total of 228 participants were included in this study. a multiplex rt-pcr was used for the detection of dientamoeba fragilis, giardia intestinalis, cryptosporidium sp. and ... | 2017 | 28007484 |
| data on rad51 amino acid sequences from higher and lower eukaryotic model organisms and parasites. | this paper contains data related to the research article titled "characterization of the recombination activities of the entamoeba histolytica rad51 recombinase" (kelso et al., in press) [1]. the known and putative amino acid sequence of rad51, the central enzyme of homologous recombination, from nineteen different higher and lower eukaryotic organisms was analyzed. here, we show amino acid conservation using a multiple sequence alignment, overall sequence identities using a percent identity mat ... | 2017 | 28018950 |
| new antiprotozoal agents: synthesis and biological evaluation of different 4-(7-chloroquinolin-4-yl) piperazin-1-yl)pyrrolidin-2-yl)methanone derivatives. | in an endeavor to develop efficacious antiprotozoal agents 4-(7-chloroquinolin-4-yl) piperazin-1-yl)pyrrolidin-2-yl)methanone derivatives (5-14) were synthesized, characterized and biologically evaluated for antiprotozoal activity. the compounds were screened in vitro against the hm1: imss strain of entamoeba histolytica and nf54 chloroquine-sensitive strain of plasmodium falciparum. among the synthesized compounds six exhibited promising antiamoebic activity with ic50 values (0.14-1.26μm) lower ... | 2017 | 28027871 |
| molecular diagnosis of entamoeba spp. versus microscopy in the great cairo. | amoebiasis is a human disease produced by entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. the true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as entamoeba dispar, and entamoeba moshkovskii. to overcome microscopy misid ... | 2017 | 28030342 |
| amoebic liver abscess in northern sri lanka: first report of immunological and molecular confirmation of aetiology. | since 1985, amoebic liver abscess (ala) has been a public health problem in northern sri lanka. clinicians arrive at a diagnosis based on clinical and ultrasonographic findings, which cannot differentiate pyogenic liver abscess (pla) from ala. as the treatment and outcome of the ala and pla differs, determining the etiological agent is crucial. | 2017 | 28061872 |
| muc2 mucin and butyrate contribute to the synthesis of the antimicrobial peptide cathelicidin in response to entamoeba histolytica- and dextran sodium sulfate-induced colitis. | embedded in the colonic mucus are cathelicidins, small cationic peptides secreted by colonic epithelial cells. humans and mice have one cathelicidin-related antimicrobial peptide (cramp) each, ll-37/hcap-18 and cramp, respectively, with related structure and functions. altered production of muc2 mucin and antimicrobial peptides is characteristic of intestinal amebiasis. the interactions between muc2 mucin and cathelicidins in conferring innate immunity against entamoeba histolytica are not well ... | 2017 | 28069814 |
| prevalence and risk factors for intestinal parasitic infection in schoolchildren in battambang, cambodia. | most intestinal parasitic infections (ipis), commonly endemic in tropical resource-poor developing countries, are neglected tropical diseases. parasitic infections and malnutrition are most commonly found in children. we determined the prevalence of ipis and the risk factors in battambang province, northwestern cambodia, from august to september 2015. this study collected 308 valid questionnaires and specimens from dontri (173, 56.2%) and kon kaêk (135, 43.8%) primary schools. all stool samples ... | 2017 | 28070012 |
| cytosine dna methylation at promoter of non ltr retrotransposon and heat shock protein gene (hsp70) of entamoeba histolytica and lack of correlation with transcription status. | non ltr retrotransposons (ehlines and ehsines) occupy 11% of the entamoeba histolytica genome. since promoter dna methylation at cytosines has been correlated with transcriptional silencing of transposable elements in model organisms we checked whether this was the case in ehline1. we located promoter activity in a 841bp fragment at 5'-end of this element by luciferase reporter assay. from rnaseq and rt-pcr analyses we selected a transcriptionally active and silent copy to study cytosine dna met ... | 2017 | 28082182 |
| a study on hla-dr/dq typing in adult malay patients with acute amoebic liver abscess. | amoebiasis is a parasitic disease caused by entamoeba histolytica that may lead to death in developing countries. few important risk factors have been identified in the development of amoebic liver abscess (ala). there are limited reports that suggest an association between antigens of the major histocompatibility complex (mhc) particularly class ii antigens and ala development. this present work aimed at studying the possible association of hla antigens with ala and disease severity. results of ... | 2016 | 28087959 |
| nanoformulation of synergistic tlr ligands to enhance vaccination against entamoeba histolytica. | diarrheal infectious diseases represent a major cause of global morbidity and mortality. there is an urgent need for vaccines against diarrheal pathogens, especially parasites. modern subunit vaccines rely on combining a highly purified antigen with an adjuvant to increase their efficacy. in the present study, we evaluated the ability of a nanoliposome adjuvant system to trigger a strong mucosal immune response to the entamoeba histolytica gal/galnac lectin leca antigen. cba/j mice were immunize ... | 2017 | 28089548 |
| iron responsive-like elements in the parasite entamoeba histolytica. | in entamoeba histolytica, iron modulates virulence and gene expression via unknown regulatory mechanisms. the existence of a posttranscriptional iron regulatory system parallel to the ire/irp (iron-responsive element/iron regulatory protein) system in the protozoan trichomonas vaginalis has recently been reported. due to their evolutionary closeness and the importance of iron for growth and virulence in these protozoa, we hypothesized the existence of an ire/irp-like mechanism in e. histolytica. ... | 2017 | 28100304 |