Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| distribution and characterization of borrelia burgdorferi isolates from ixodes scapularis and presence in mammalian hosts in ontario, canada. | the blacklegged tick, ixodes scapularis say (acari: ixodidae), has a wide geographical distribution in ontario, canada, with a detected range extending at least as far north as the 50th parallel. our data of 591 adult i. scapularis submissions collected from domestic animals (canines, felines, and equines) and humans during a 10-yr period (1993-2002) discloses a monthly questing activity in ontario that peaks in may and october. the lyme disease spirochete borrelia burgdorferi johnson, schmidt, ... | 2006 | 16892637 |
| the lyme vaccine: a cautionary tale. | people living in endemic areas acquire lyme disease from the bite of an infected tick. this infection, when diagnosed and treated early in its course, usually responds well to antibiotic therapy. a minority of patients develops more serious disease, particularly after a delay in diagnosis or therapy, and sometimes chronic neurological, cardiac, or rheumatological manifestations. in 1998, the fda approved a new recombinant lyme vaccine, lymerix, which reduced new infections in vaccinated adults b ... | 2007 | 16893489 |
| anti-cd25 antibody treatment of mice vaccinated and challenged with borrelia spp. does not exacerbate arthritis but inhibits borreliacidal antibody production. | cd4(+) cd25(+) t cells are a population of regulatory t cells responsible for the modulation of the immune response in several autoimmune and infectious disease models. we previously showed that adoptive transfer of enriched cd4(+) cd25(+) t cells also plays a major role in the prevention of arthritis in borrelia-vaccinated (borrelia burgdorferi isolate 297) and -challenged (b. bissettii) mice. here, we present evidence that administration of anti-cd25 antibody at the time of challenge or at lat ... | 2006 | 16893988 |
| fundamental processes in the evolutionary ecology of lyme borreliosis. | the evolutionary ecology of many emerging infectious diseases, particularly vector-borne zoonoses, is poorly understood. here, we aim to develop a biological, process-based framework for vector-borne zoonoses, using borrelia burgdorferi sensu lato (s.l.), the causative agent of lyme borreliosis in humans, as an example. we explore the fundamental biological processes that operate in this zoonosis and put forward hypotheses on how extrinsic cues and intrinsic dynamics shape b. burgdorferi s.l. po ... | 2006 | 16894341 |
| [seasonal activity of the ticks ixodes ricinus in the lakesides and forests habitats of szczecin and environs and its infestation with the spirochete borrelia burgdorferi]. | the article presents the results of researche (1999-2000) on numbers and seasonal activity of ixodes ricinus in selected lakesides and forests habitats of szczecin and on occurence of the spirochetes borrelia burgdorferi sensu lato in ticks. a total of 8065 ticks were collected from 6 localities : 647 larvae, 4549 nymphs, 1624 females and 1244 males. the spirochetes b. burgdorferi were found (using the pcr techniques) in 2271 specimens e.c. 28.1% whole population. numbers infected larvae were 21 ... | 2001 | 16894752 |
| [usefulness of the molecular techniques for detecting and/or identifing of parasites and fungi in humans and animals or pathogens transmitted by ticks. part iii]. | 2001 | 16894762 | |
| babesia microti and borrelia burgdorferi coinfection associated with increased severity of arthritis. | 2006 | 16897675 | |
| effect of salivary gland extract from ixodes ricinus ticks on the proliferation of borrelia burgdorferi sensu stricto in vivo. | saliva-activated transmission (sat) of borrelia burgdorferi sensu stricto was demonstrated using real-time pcr and salivary gland extract (sge) from partially fed ixodes ricinus ticks. c3h/hen mice were injected intradermally with 1.5 x 10(3) spirochetes mixed with 40 microg of sge per mouse. the control group was inoculated with the same dose of spirochetes without sge. the accelerating effect of sge on spirochete proliferation was demonstrated on day 1 post infection, when a 4.2-fold increase ... | 2006 | 16898130 |
| ixodes ricinus: the potential of two-dimensional gel electrophoresis as a tool for studying host-vector-pathogen interactions. | ixodes ricinus is a three-host tick, with three active instars. for moulting to occur the tick has to find a host where it can take a blood meal. throughout feeding i. ricinus can be infected or infect the host with different pathogens, e.g., tick-borne encephalitis virus or borrelia burgdorferi. the host-vector-pathogen interaction is very complex, making a detailed study difficult. here we analyse the potential of two-dimensional gel electrophoresis (2de) to study the host-vector-pathogen inte ... | 2007 | 16904668 |
| [tick-borne diseases exposure of forestry workers and preventive methods usage]. | forestry workers are a group of a high risk of occupational exposure to tick-borne diseases. the group of 51 healthy persons with a history of more than 3 years forestry working was tested for antibodies against b. burgdorferi and tick-borne encephalitis (tbe) and reviewed on tick-bites preventive methods usage. two-steps serological examination revealed the anti-b. burgdorferi antibodies in 14 (27.5%) persons. anti-tbe antibodies were present in 6 (11.8%) persons. despite of multiple tick bites ... | 2006 | 16909769 |
| [specific features of borrelia burgdorferi infection in children]. | clinical picture of borrelia burgdorferi infection has been presented in 89 children from lodz region. the analysis showed significant domination of cases with non specific symptoms (41.6%) as: fever or headache and cases with affected central and peripheral nervous system (30.3%). peripheral cranial nerves paralysis and symptoms of cerebrospinal meningitis dominated among children with neuroborreliosis. unlike descriptions concerning adults, majority of the observed symptoms were changes charac ... | 2006 | 16909770 |
| [different diagnosis:lyme arthritis or rheumatoid arthritis]. | 2006 | 16909777 | |
| [assessment of antioxidative defense parameters in serum and age of patients showing antibodies anti-borrelia burgdorferi presence--non-symptomatic carriers]. | to evaluate in serum antioxidative deffence parameters--sod ec.1.15.1.1 (superoxide dismutase), gsh-px ec.1.15.1.1 (glutathine peroxidase), -sh (sulphhydryl groups) among patients previously diagnosed with borreliosis presently showing antibodies against borrelia burgdorferi presence. the antioxidative deffence parameters were evaluated in 117 sera of patients with previously diagnosed borreliosis aged x = 43,07. control group consisted of 39 persons aged x = 40,97 with no detectable anti-borrel ... | 2006 | 16909786 |
| [diagnosis and treatment of lyme arthritis]. | lyme disease is a multisystem disease that affects primarily skin, nervous system, heart and joins. lyme disease is caused by borrelia burgdorferi and spread by ixodes ticks. arthritis is a well-known manifestation of lyme disease (ld). joint symptoms associated with b. burgdorferi infection range from arthralgias, to brief attacks of arthritis, to chronic erosive synovitis. arthritis may present as oligo- or monoarthritis and typically causes intermittent attacks of oligoarticular arthritis in ... | 2006 | 16909789 |
| [new aspects of pathogenesis of lyme borreliosis]. | b. burgdorferi can evade the destructive effects of the immune system by binding host's complement regulators, which leads to inhibition of the complement activation cascade. complement activity is blocked by crasps--complement regulator acquiring surface proteins. complement resistance might therefore represent one major pathogenic factor favoring spirochete transmission to the vertebrate host, as well as determine host reservoirs of borrelia burgdorferi genospecies. the cause of neuro-psychiat ... | 2006 | 16909797 |
| [comparison of two types of diagnostic test detecting antibodies against borrelia burgdorferi: eia based on one genospecies antigens and elisa based on recombinant antigens]. | the aim of the study was to compare the results of elisa diagnostic kit detecting antibodies against b. burgdorferi based on combination of three genospecies recombinant and eia kits based on one of three genospecies. sera of 351 forest workers were evaluated with elisa kit (recombinant antigen, igg). seropositive samples were tested with eia kits based on b. burgdorferi s.s., b. garinii, b. afzelii antigens. diagnostic kits based on combination of antigens of three genospecies more often detect ... | 2006 | 16909798 |
| [western-blot with vlse protein and "in vivo" antigens in lyme borreliosis diagnosis]. | the aim of the study was the evaluation of the efficiency of western blot (ecoline) test detecting simoultanous presence of igm and igg antibodies against b. burgdorferi in diagnosis of early and late stage of lyme borreliosis. the comparison of results achieved by performing test western-blot, elisa (based on recombinant antigens of three genospecies of borrelia) and eia (based on antigens of one b. burgdorferi genospecies). the tests western blot: ecoline (virotech) with antygens "in vivo", el ... | 2006 | 16909799 |
| ixodes frontalis and avian tick-related syndrome in the united kingdom. | this study aimed to characterise tick species responsible for avian tick infestations in the uk, to analyse various risk factors for tick-related syndrome in tick-infested birds and to test samples for the presence of certain tick-transmitted pathogens. | 2006 | 16911113 |
| comparison of findings for patients with borrelia garinii and borrelia afzelii isolated from cerebrospinal fluid. | the most common cause of lyme neuroborreliosis in europe is borrelia garinii, followed by borrelia afzelii. however, no series describing patients with culture-confirmed cases of lyme neuroborreliosis have been published, and no comparison of findings for patients with b. garinii and b. afzelii isolated from cerebrospinal fluid (csf) has been reported. | 2006 | 16912943 |
| comparative genome analysis: selection pressure on the borrelia vls cassettes is essential for infectivity. | at least three species of borrelia burgdorferi sensu lato (bbsl) cause tick-borne lyme disease. previous work including the genome analysis of b. burgdorferi b31 and b. garinii pbi suggested a highly variable plasmid part. the frequent occurrence of duplicated sequence stretches, the observed plasmid redundancy, as well as the mainly unknown function and variability of plasmid encoded genes rendered the relationships between plasmids within and between species largely unresolvable. | 2006 | 16914037 |
| opsoclonus-myoclonus as a manifestation of lyme disease. | opsoclonus-myoclonus syndrome (oms) is a rare condition that includes chaotic multidirectional saccadic eye movements associated with myoclonus and ataxia. in adults, it is usually considered to be an autoimmune disease occurring either in a paraneoplastic context or after central nervous system infection. we report the case of a patient who presented with the classic features of oms as a manifestation of acute borrelia burgdorferi infection that was shown both on serum and cerebrospinal fluid e ... | 2006 | 16914760 |
| glycoconjugates: roles in neural diseases caused by exogenous pathogens. | numerous reports indicate that lipid or protein associated carbohydrates are essential for infection of cells by various viruses, bacteria, or bacterial toxins, some of which affect the nervous system. examples of such pathogens include tetanus and botulinum neurotoxin, shiga and shiga-like toxins, borrelia burgdorferi, mycobacterium leprae, and human immunodeficiency virus. this review discusses evidence indicating that carbohydrates are essential for these pathogens to induce their deleterious ... | 2006 | 16918390 |
| natural killer t cells recognize diacylglycerol antigens from pathogenic bacteria. | natural killer t (nkt) cells recognize glycosphingolipids presented by cd1d molecules and have been linked to defense against microbial infections. previously defined foreign glycosphingolipids recognized by nkt cells are uniquely found in nonpathogenic sphingomonas bacteria. here we show that mouse and human nkt cells also recognized glycolipids, specifically a diacylglycerol, from borrelia burgdorferi, which causes lyme disease. the b. burgdorferi-derived, glycolipid-induced nkt cell prolifera ... | 2006 | 16921381 |
| borrelial lymphocytoma cutis successfully treated with intralesional interferon-alpha-2a. | 2006 | 16922975 | |
| comparative genome hybridization reveals substantial variation among clinical isolates of borrelia burgdorferi sensu stricto with different pathogenic properties. | clinical and murine studies suggest that there is a differential pathogenicity of different genotypes of borrelia burgdorferi, the spirochetal agent of lyme disease. comparative genome hybridization was used to explore the relationship between different genotypes. the chromosomes of all studied isolates were highly conserved (>93%) with respect to both sequence and gene order. plasmid sequences were substantially more diverse. plasmids lp54, cp26, and cp32 were present in all tested isolates, an ... | 2006 | 16923879 |
| nkt cells join the war on lyme disease. | 2006 | 16924252 | |
| functional characterization of bbcrasp-2, a distinct outer membrane protein of borrelia burgdorferi that binds host complement regulators factor h and fhl-1. | borrelia burgdorferi, the aetiological agent of lyme disease, employs sophisticated means to survive in diverse mammalian hosts. recent studies demonstrated that acquisition of complement regulators factor h and factor h-like protein-1 (fhl-1) allows spirochetes to resist complement-mediated killing. serum-resistant b. burgdorferi express up to five distinct complement regulator-acquiring surface proteins (crasps) that bind factor h and/or fhl-1. in this study we have identified and characterize ... | 2006 | 16925556 |
| constitutive expression of outer surface protein c diminishes the ability of borrelia burgdorferi to evade specific humoral immunity. | the lyme disease spirochete borrelia burgdorferi reduces the expression of outer surface protein c (ospc) in response to the development of an anti-ospc humoral response, leading to the hypothesis that the ability to repress ospc expression is critical for the pathogen to proceed to chronic infection. b. burgdorferi was genetically modified to constitutively express ospc by introducing an extra ospc copy fused with the borrelial flagellar gene (flab) promoter. such a genetic modification did not ... | 2006 | 16926410 |
| [lyme borreliosis situation in north africa]. | many epidemiological studies were conducted for studying lyme borreliosis (lb) which represents a new global public health problem. it is now the most common vector-borne disease in europe and north america. the causative agent borrelia burgdorferi sl is a bacterial species complex comprising 12 delineated and named species. in north africa, few studies based on clinical and serological features, have suggested that lb could occur. indeed, recent studies conducted in tunisia, algeria and morocco ... | 2004 | 16929760 |
| bilateral facial palsy: epstein-barr virus, not lyme disease. | bilateral facial palsy is frequently linked with lyme disease. we report a patient with bilateral facial palsy due to epstein-barr virus infection but with borrelia burgdorferi igm in serum caused by polyclonal b-lymphocyte stimulation. | 2006 | 16930373 |
| characterization of the humoral immune response in dogs after vaccination against the lyme borreliosis agent a study with five commercial vaccines using two different vaccination schedules. | using five commercially available vaccines, groups of dogs were vaccinated against lyme borreliosis and followed for 13 months. a modified vaccination schedule was included in the study in an attempt to induce higher and therefore longer-lasting protective antibody levels during the first year of immunization. following vaccination antibodies were monitored using elisa and western blotting. serum samples were examined either with antigen preparations derived from either borrelia burgdorferi s. s ... | 2007 | 16930782 |
| differential telomere processing by borrelia telomere resolvases in vitro but not in vivo. | causative agents of lyme disease and relapsing fever, including borrelia burgdorferi and borrelia hermsii, respectively, are unusual among bacteria in that they possess a segmented genome with linear dna molecules terminated by hairpin ends, known as telomeres. during replication, these telomeres are processed by the essential telomere resolvase, rest, in a unique biochemical reaction known as telomere resolution. in this study, we report the identification of the b. hermsii rest gene through cr ... | 2006 | 16936037 |
| [first aid in borreliosis]. | 2006 | 16937867 | |
| interaction of a neurotropic strain of borrelia turicatae with the cerebral microcirculation system. | relapsing fever (rf) is a spirochetal infection characterized by relapses of a febrile illness and spirochetemia due to the sequential appearance and disappearance of isogenic serotypes in the blood. the only difference between isogenic serotypes is the variable major outer membrane lipoprotein. in the absence of specific antibody, established serotypes cause persistent infection. studies in our laboratory indicate that another consequence of serotype switching in rf is a change in neuroinvasive ... | 2006 | 16940140 |
| [laboratory diagnosis of infection caused by borrelia burgdorferi]. | the laboratory diagnosis of lyme disease in denmark is reviewed with recommendations for serological testing. in denmark the laboratory testing is performed with an elisa technique. most laboratories use an assay based on purified flagella antigen. the two-tier approach with western blot as confirmatory testing is not recommended since the contribution to the diagnostic specificity is only marginal. predictive values of lyme serology are presented, based on the estimated prevalence of the differ ... | 2006 | 16942701 |
| [lyme borreliosis]. | lyme borreliosis is a zoonotic disease transmitted by ticks and caused by the spirochete borrelia burgdorferi. there are cases of the infection reported at least since the beginning of last century, although a complete description of the disease is available only since 1982. clinical presentation consists generally of a characteristic skin rash, known as erythema migrans, and a variety of systemic manifestations, which evolve in stages, along months to years. diagnosis is usually made with serol ... | 2006 | 16942731 |
| transcriptional profiling of borrelia burgdorferi containing a unique bosr allele identifies a putative oxidative stress regulon. | borrelia burgdorferi regulates gene expression in response to environmental conditions, including temperature, ph, redox potential and host factors. b. burgdorferi encodes a perr homologue designated bosr, which presumably serves as a global regulator of genes involved in the oxidative stress response. infectious b. burgdorferi strain b31 is resistant to oxidative stressors in vitro, whereas the non-infectious isolate was sensitive due, in part, to a point mutation that converts an arginine to a ... | 2006 | 16946255 |
| parry-romberg syndrome associated with borreliosis: could photochemotherapy halt the progression of the disease? | parry-romberg syndrome (prs) or progressive hemifacial atrophy is a rare entity characterized by unilateral atrophy of the skin, subcutaneous tissue and sometimes bone and cartilage. although this syndrome has overlapping features of scleroderma 'en coup de sabre', it shows little or no sclerosis and may affect the entire distribution of the trigeminal nerve including the eye and tongue. as the pathogenesis is unknown, no effective therapy exists. we present here the third case of prs associated ... | 2006 | 16948828 |
| the effect of pre-calving environment on the development of digital dermatitis in first lactation heifers. | digital dermatitis is commonly reported to be most severe in first lactation heifers. it has been suggested that this initial infection is followed by the development of a limited immunity to the organisms which cause digital dermatitis. if this is the case then exposure to digital dermatitis prior to calving should reduce its severity after calving. a study was undertaken to examine whether such exposure significantly affected the development of digital dermatitis post-partum. twelve weeks prio ... | 2007 | 16949318 |
| the dbpba locus of borrelia burgdorferi is not essential for infection of mice. | the lyme disease spirochete borrelia burgdorferi expresses a broad array of adhesive molecules, including the decorin-binding proteins a and b (dbpa and dbpb), which are believed to play important roles in mammalian infection. the dbpba locus was deleted; resulting mutants were able to infect both immunodeficient and immunocompetent mice, indicating that neither dbpa nor dbpb is essential for the infection of mammals, although the dbpab deficiency may significantly attenuate infectivity potentia ... | 2006 | 16954404 |
| treatment of lichen sclerosus with antibiotics. | current therapy for lichen sclerosus centers on topical steroids, particularly clobetasol propionate. as some evidence suggests an infectious etiology owing to borrelia, we studied the effect of penicillin and cephalosporin therapy on patients with lichen sclerosus who had responded poorly to treatment with potent topical corticosteroids. fifteen patients with lichen sclerosus were treated for 3-21 months with either penicillin or cephalosporins in an observational study. thirteen patients (nine ... | 2006 | 16961523 |
| molecular divergences of the ornithodoros sonrai soft tick species, a vector of human relapsing fever in west africa. | the soft tick ornithodoros sonrai is recognized as the only vector of borrelia crocidurae causing human relapsing fever in west africa. its determination has been exclusively based on morphological features, geographical distribution and vector competence. some ambiguities persist in its systematics and may cause misunderstanding about west african human relapsing fevers epidemiology. by amplifying and aligning 16s and 18s rdna genes in o. sonrai specimens collected from 14 distinct sites in sen ... | 2006 | 16962358 |
| epidemiology of relapsing fever borreliosis in europe. | tick-borne relapsing fever is a bacterial infection caused by spirochetes of the genus borrelia. this zoonotic disease is transmitted to humans through the bite of soft ticks of the genus ornithodoros. it is responsible for recurring fever access associated with spirochetemia. we present here an overview of tick-borne relapsing fever occurring in europe, as well as of the potential threat to travellers. | 2006 | 16965346 |
| characterization of a unique borreliacidal epitope on the outer surface protein c of borrelia burgdorferi. | the outer surface protein c (ospc) of the lyme disease agent, borrelia burgdorferi, is an immunoprotective antigen in laboratory models of infection. however, to understand its protective effects, it is important to identify the key epitopes of this protein. we produced a borreliacidal anti-ospc monoclonal antibody specific to the b31 strain and identified its binding site. the specificity of mab 16.22 was determined by western blot reactivity using ospc derived from different borrelia isolates ... | 2006 | 16965353 |
| a morphealike skin condition caused by borrelia burgdorferi in an immunocompromised patient. | 2006 | 16970224 | |
| [study on the coinfection status of borrelia burgdorferi sensu lato and spotted fever group rickettsia in ticks from hunchun, jilin province]. | to understand the coinfection status of borrelia burgdorferi sensu lato (b.b.s.l) and spotted fever group rickettsia (sfgr) in hunchun of jilin province, china. | 2006 | 16981329 |
| the flagellar cytoskeleton of the spirochetes. | the recent discoveries of prokaryotic homologs of all three major eukaryotic cytoskeletal proteins (actin, tubulin, intermediate filaments) have spurred a resurgence of activity in the field of bacterial morphology. in spirochetes, however, it has long been known that the flagellar filaments act as a cytoskeletal protein structure, contributing to their shape and conferring motility on this unique phylum of bacteria. therefore, revisiting the spirochete cytoskeleton may lead to new paradigms for ... | 2006 | 16983197 |
| control of borrelia burgdorferi-specific cd4+-t-cell effector function by interleukin-12- and t-cell receptor-induced p38 mitogen-activated protein kinase activity. | infection with borrelia burgdorferi, the causative agent of lyme disease, results in a th1 response and proinflammatory cytokine production. mice deficient for mkk3, an upstream activator of p38 mitogen-activated protein (map) kinase, develop a lower th1 response and exhibit an impaired ability to produce proinflammatory cytokines upon infection with the spirochete. we investigated the contribution of p38 map kinase activity in gamma interferon (ifn-gamma) production in cd4+ t cells in response ... | 2006 | 16988247 |
| interleukin-10 anti-inflammatory response to borrelia burgdorferi, the agent of lyme disease: a possible role for suppressors of cytokine signaling 1 and 3. | it has been established that interleukin-10 (il-10) inhibits inflammatory cytokines produced by macrophages in response to borrelia burgdorferi or its lipoproteins. the mechanism by which il-10 exerts this anti-inflammatory effect is still unknown. recent findings indicate that suppressors of cytokine signaling (socs) proteins are induced by cytokines and toll-like receptor (tlr)-mediated stimuli, and in turn they can down-regulate cytokine and tlr signaling in macrophages. because it is known t ... | 2006 | 16988256 |
| borrelia afzelii gene expression in ixodes ricinus (acari: ixodidae) ticks. | spirochetes belonging to the borrelia burgdorferi sensu lato (s. l.) complex have evolved remarkable ability to survive in diverse ecological niches during transmission cycles between ticks and vertebrate hosts by variable gene expression. to understand the events during spirochete transmission from feeding ticks to hosts, mrna levels of selected b. afzelii genes (bbk32, dbpa, ospa, ospc and vlse) were measured by quantitative real-time sybr green pcr. b. afzelii infected ixodes ricinus nymphs f ... | 2006 | 16989569 |
| a lightcycler taqman assay for detection of borrelia burgdorferi sensu lato in clinical samples. | lyme disease (ld) is an infection caused by an ixodid tick-borne spirochete, borrelia burgdorferi sensu lato. ld manifests itself as a multisystem inflammatory disease that affects the skin in its early localized stage and spreads to the joints, nervous system, heart, and, to a lesser extent, other organ systems in its later disseminated stages. if diagnosed and treated early with appropriate antibiotics, ld is almost always readily cured. developing a highly sensitive and specific real-time pol ... | 2007 | 16989975 |
| refractoriness of the western fence lizard (sceloporus occidentalis) to the lyme disease group spirochete borrelia bissettii. | the western fence lizard, sceloporus occidentalis, is refractory to experimental infection with borrelia burgdorferi sensu stricto, one of several lyme disease spirochetes pathogenic for humans. another member of the lyme disease spirochete complex, borrelia bissettii, is distributed widely throughout north america and a similar, if not identical, spirochete has been implicated as a human pathogen in southern europe. to determine the susceptibility of s. occidentalis to b. bissettii, 6 naïve liz ... | 2006 | 16995383 |
| transfer of borrelia burgdorferi s.s. infection via blood transfusion in a murine model. | without antibiotic treatment, the lyme-disease-causing bacterium, borrelia burgdorferi can be cultured from the peripheral blood of human patients nearly 6 wk post-tick bite. to determine if lyme disease spirochetes can be transmitted from a spirochetemic donor mouse to a naive recipient during blood transfusion, blood taken from immunocompetent infected mice was transfused into either immunodeficient (scid) mice, inbred immunocompetent animals (c3h/hej), or outbred mice. nine of 19 (47.7%) immu ... | 2006 | 16995409 |
| development of an ospc-based tetravalent, recombinant, chimeric vaccinogen that elicits bactericidal antibody against diverse lyme disease spirochete strains. | lyme disease is the most common arthropod-borne disease in north america and europe. at present, there is no commercially available vaccine for use in humans. outer surface protein c (ospc) has antigenic and expression characteristics that make it an attractive vaccine candidate; however, sequence heterogeneity has impeded its use as a vaccinogen. sequence analyses have identified 21 well defined ospc phyletic groups or "types" (designated a-u). in this report we have mapped the linear epitopes ... | 2007 | 16996663 |
| unexpected twist: harnessing the energy in positive supercoils to control telomere resolution. | negative dna supercoiling is an important conformational property of bacterial dna that plays a significant role in a wide variety of dna transactions. in contrast, positive dna supercoiling is a by-product of cellular processes that involve helical unwinding or movement of dna by a fixed translocase, and has generally been considered a necessary evil requiring removal. we now report the first evidence suggesting a physiological role for positive supercoiling; this occurs in telomere resolution ... | 2006 | 16999829 |
| borrelia burgdorferi complement regulator-acquiring surface protein 1 of the lyme disease spirochetes is expressed in humans and induces antibody responses restricted to nondenatured structural determinants. | borrelia burgdorferi complement regulator-acquiring surface protein 1 (crasp-1), the dominant factor h and fhl-1-binding protein of the lyme disease spirochete b. burgdorferi, is implicated in pathogen persistence and was recently reported to be nonimmunogenic in humans. here we show that serum samples from lyme disease patients contain antibodies with exclusive specificity for nondenatured structural determinants of crasp-1. | 2006 | 17000728 |
| [clinico-pathological collations in borreliosis]. | borreliosis is an infectious disease caused by spirochetal microorganisms borrelia burgdorferi transmitted by ticks. due to versatile clinical symptomatology and many pathogenetic aspects not explained yet, diagnosis of this disease is often very difficult. borreliosis may affect various human organs and systems such as movement system, nervous system (central and peripheral), skin, heart and vessels. one of the diagnostic methods for detection of microbial organisms in tissues is the histochemi ... | 2006 | 17007281 |
| clinical appearance of erythema migrans caused by borrelia afzelii and borrelia garinii--effect of the patient's sex. | the aim in this survey was to study the clinical characteristics of infections caused by borrelia genospecies in patients with erythema migrans where borrelial origin was confirmed by polymerase chain reaction. the aim was also to study factors influencing the clinical appearance of erythema migrans. | 2006 | 17009065 |
| therapy for lyme arthritis: strategies for the treatment of antibiotic-refractory arthritis. | 2006 | 17009226 | |
| role of aggrecanase 1 in lyme arthritis. | arthritis is one of the hallmarks of late-stage lyme disease. previous studies have shown that infection with borrelia burgdorferi, the causative agent of lyme disease, results in degradation of proteoglycans and collagen in cartilage. b burgdorferi do not appear to produce any exported proteases capable of digesting proteoglycans and collagen, but instead, induce and activate host proteases, such as matrix metalloproteinases (mmps), which results in cartilage degradation. the role of aggrecanas ... | 2006 | 17009305 |
| [tick infestation and the prevalence of borrelia burgdorferi and babesia divergens in cattle in bavaria]. | during the grazing period 2002 319 cattle from 31 farms located in 6 districts of southern bavaria were examined for the presence of ticks in 4- to 5-week intervals, and 287 serum samples were tested for the presence of antibodies against borrelia burgdorferi and babesia divergens. ticks were detected in all 31 farms with a mean prevalence of 69%. 3218 out of 3453 collected ticks were ixodes ricinus; 139 nymphs, 19 larvae and 77 damaged adult specimens could only be determined to the genus level ... | 2006 | 17009719 |
| identification of potential virulence determinants by himar1 transposition of infectious borrelia burgdorferi b31. | lyme disease borrelia organisms are highly invasive spirochetes that alternate between vertebrate and arthropod hosts and that establish chronic infections and elicit inflammatory reactions in mammals. although progress has been made in the targeted mutagenesis of individual genes in infectious borrelia burgdorferi, the roles of the vast majority of gene products in pathogenesis remain unresolved. in this study, we examined the feasibility of using transposon mutagenesis to identify infectivity- ... | 2006 | 17015459 |
| motion-onset and pattern-reversal visual evoked potentials in diagnostics of neuroborreliosis. | neuroborreliosis is a form of borreliosis that affects the central and/or peripheral nervous system. although it can mimic neurologic and ophthalmologic disorders such as multiple sclerosis and optic neuritis, visual evoked potential (vep) examination is usually not used in neuroborreliosis diagnostics. combined vep testing (pattern-reversal veps and veps produced in response to linear and radial motion) was performed in 81 patients with neuroborreliosis verified by laboratory results (positive ... | 2006 | 17016151 |
| prevalence of borrelia burgdorferi in small mammals in new york state. | intensive small mammal trapping was conducted in 12 counties in new york state during 1998-2000 to investigate the prevalence and site specificity of the lyme disease spirochete, borrelia burgdorferi in, and presence of the blacklegged tick, ixodes scapularis say on, the wild mice peromyscus leucopus rafinesque and peromyscus maniculatus wagner and other small mammal species. previously captured mice (1992-1997) from throughout new york state also were recruited into the study, providing a total ... | 2006 | 17017230 |
| tick inoculation in an eyelid region: report on five cases with one complication of the orbital myositis associated with lyme borreliosis. | to determine the frequency and dependence of lyme borreliosis after tick infestation in the eyelid region. | 2006 | 17020000 |
| identification of borrelia burgdorferi ribosomal protein l25 by the phage surface display method and evaluation of the protein's value for serodiagnosis. | the phage surface display technique was used to identify borrelia burgdorferi antigens. by affinity selection with immunoglobulin g from pooled sera of six lyme borreliosis (lb) patients, the ribosomal protein l25 was identified. the diagnostic value of l25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 lb patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify l25 as a useful antigen when combined w ... | 2006 | 17021109 |
| in vitro susceptibility of isolates of borrelia burgdorferi s.l. to antimicrobial agents. | in the present study, we investigate the in vitro antimicrobial activity of macrolides, beta-lactams and tetracycline against borrelia burgdorferi s.l. clinical and tick isolates. minimal inhibitory concentrations (mics) were determined in normal growth condition and after pre-exposure of the strains to sub-mic of the founder of each drug family. all the classes of tested antibiotics showed good antibacterial activity against all the borreliae isolates and there were no significant susceptibilit ... | 2006 | 17026839 |
| analysis of antibody response in humans to the type a ospc loop 5 domain and assessment of the potential utility of the loop 5 epitope in lyme disease vaccine development. | the ospc protein of borrelia burgdorferi is an immunodominant antigen. here we demonstrate that the loop 5 domain of type a ospc is surface exposed, elicits bactericidal antibody in mice, and is antigenic in humans. the data suggest that loop 5 may be suitable for inclusion in a polyvalent, chimeric ospc vaccinogen. | 2006 | 17028218 |
| serologic evaluation of patients from missouri with erythema migrans-like skin lesions with the c6 lyme test. | southern tick-associated rash illness (stari), also known as masters disease, affects people predominantly in the southeast and south central united states. these patients exhibit skin lesions that resemble erythema migrans (em), the characteristic skin lesion in early lyme disease. the etiology of stari remains unknown, and no serologic test is available to aid in its diagnosis. the c6 lyme enzyme-linked immunosorbent assay was used to evaluate coded serum specimens from patients with stari at ... | 2006 | 17028220 |
| longitudinal analysis of tick densities and borrelia, anaplasma, and ehrlichia infections of ixodes ricinus ticks in different habitat areas in the netherlands. | from 2000 to 2004, ticks were collected by dragging a blanket in four habitat areas in the netherlands: dunes, heather, forest, and a city park. tick densities were calculated, and infection with borrelia burgdorferi and anaplasma and ehrlichia species was investigated by reverse line blot analysis. the lowest tick density was observed in the heather area (1 to 8/100 m2). in the oak forest and city park, the tick densities ranged from 26 to 45/100 m2. the highest tick density was found in the du ... | 2006 | 17028227 |
| dual role of myd88 in rapid clearance of relapsing fever borrelia spp. | relapsing fever borrelia spp. undergo antigenic variation, achieve high levels in blood, and require rapid production of immunoglobulin m (igm) for clearance. myd88-deficient mice display defective clearance of many pathogens; however, the igm response to persistent infection is essentially normal. therefore, myd88(-/-) mice provided a unique opportunity to study the effect of nonantibody, innate host defenses to relapsing fever borrelia. infected myd88(-/-) mice harbored extremely high levels o ... | 2006 | 17030581 |
| antigenicity of borrelial protein bbk32 fragments in early lyme borreliosis. | recombinantly produced borrelial bbk32 protein fragments originating from borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii were evaluated as antigens in the serology of lyme borreliosis (lb). in elisa, a mid-portion hydrophilic fragment reacted with lb patient sera. of the 23 patients with culture- or pcr-positive erythema migrans (em), 43 % at diagnosis and 52 % at convalescence were positive for at least one borrelia species-specific variant bbk32 fragment antigen. in ... | 2006 | 17030908 |
| central nervous system borreliosis mimicking a pontine tumour. | in childhood, facial nerve palsy and headache are typical symptoms of second and third stage neuroborreliosis. while focal demyelination is occasionally observed on mri scans, the appearance of a tumorous lesion is extremely rare. the case of a 10-year-old girl with neuroborreliosis mimicking a space-occupying lesion in the brainstem, without any previously recognized manifestations of borreliosis, is reported. | 2006 | 17030923 |
| vector seasonality, host infection dynamics and fitness of pathogens transmitted by the tick ixodes scapularis. | fitness of tick-borne pathogens may be determined by the degree to which their infection dynamics in vertebrate hosts permits transmission cycles if infective and uninfected tick stages are active at different times of the year. to investigate this hypothesis we developed a simulation model that integrates the transmission pattern imposed by seasonally asynchronous nymphal and larval ixodes scapularis ticks in northeastern north america, with a model of infection in white-footed mice (peromyscus ... | 2007 | 17032476 |
| prevalence of borrelia burgdorferi s.l. and anaplasma phagocytophilum in the wood tick ixodes ricinus in the province of trento, italy. | 2006 | 17033789 | |
| borrelia burgdorferi genetic markers and disseminated disease in patients with early lyme disease. | three genetic markers of borrelia burgdorferi have been associated with disseminated disease: the ospc type, the 16s-23s rrna intergenic spacer type (rst), and vlse. here, we modified previous methods so as to identify the three markers by pcr and restriction fragment length polymorphism in parallel, analyzed b. burgdorferi isolates from erythema migrans (em) skin lesions in 91 patients, and correlated the results with evidence of dissemination. ospc type a was found approximately twice as frequ ... | 2006 | 17035489 |
| chronic polyneuropathy and lyme disease. | infection of the peripheral nervous system with borrelia burgdorferi can present as a cranial neuropathy or radiculopathy with cerebrospinal fluid (csf) pleocytosis and intrathecal antibody production against b. burgdorferi, or as an asymmetric peripheral neuropathy with acrodermatitis chronica atrophicans (aca) and normal csf findings. according to north american studies, it can also present as a symmetric chronic polyneuropathy without aca or other lyme manifestations. our purpose was to inves ... | 2006 | 17038034 |
| spatial patterns of lyme disease risk in california based on disease incidence data and modeling of vector-tick exposure. | ixodes pacificus, particularly the nymphal life stage, is the primary vector to humans of the lyme disease agent borrelia burgdorferi in california. during 2004, we collected i. pacificus nymphs from 78 woodland sites in ecologically diverse mendocino county, which has a moderately high incidence of lyme disease. within this county, nymphal density was elevated in forested areas with a growing degree day range of 2,600-3,000 (10 degrees c base). using a geographic information systems approach, w ... | 2006 | 17038692 |
| disruption of the salivary protein 14 in ixodes scapularis nymphs and impact on pathogen acquisition. | we previously examined the physiological role of the anticoagulant salivary protein 14 (salp14) in adult ixodes scapularis and showed that salp14 played a role in tick feeding and engorgement. we now analyze whether the disruption of the salp14 family expression by rna interference affects tick weight in naïve nymph i. scapularis. salp14 expression after dsrna injection was significantly reduced, as shown by mrna and protein analysis. however, nymph engorgement weight was not altered in salp9pac ... | 2006 | 17038693 |
| detection of borrelia burgdorferi dna in tissues from dogs with presumptive lyme borreliosis. | to develop a quantitative pcr assay for detection of borrelia burgdorferi dna in formalin-fixed, paraffin-embedded tissues; compare results of this assay with results of immunohistochemical staining of tissues from seropositive dogs; and determine whether b burgdorferi dna could be detected in renal tissues from dogs with presumptive lyme nephritis. | 2006 | 17042727 |
| reinfection with lyme borreliosis presenting as a painful polyradiculopathy: bannwarth's, beevor's and borrelia. | 2006 | 17043300 | |
| borrelia burgdorferi sensu lato infection in mosquitoes from szczecin area. | the aim of the study was to determine the level of infection in mosquitoes with spirochetes borrelia burgdorferi sensu lato in the woody areas of szczecin. the mosquitoes were collected from may to september 2003. the spirochetes, borrelia burgdorferi s. l., present in mosquitoes were detected in mosquitoes with indirect immunofluorescence assay (ifa) using rabbit anti-borrelia burgdorferi antibodies and goat anti-rabbit igg marked with fluorescein isocyanate (fitc). a total of 1557 females and ... | 2006 | 17044261 |
| invasion of human neuronal and glial cells by an infectious strain of borrelia burgdorferi. | human infection by borrelia burgdorferi, the etiological agent for lyme disease, can result in serious acute and late-term disorders including neuroborreliosis, a degenerative condition of the peripheral and central nervous systems. to examine the mechanisms involved in the cellular pathogenesis of neuroborreliosis, we investigated the ability of b. burgdorferi to attach to and/or invade a panel of human neuroglial and cortical neuronal cells. in all neural cells tested, we observed b. burgdorfe ... | 2006 | 17045505 |
| use of cfse staining of borreliae in studies on the interaction between borreliae and human neutrophils. | species of the tick-transmitted spirochete group borrelia burgdorferi sensu lato (b. burgdorferi) cause lyme borreliosis. acute borrelial infection of the skin has unusual characteristics with only a mild local inflammatory response suggesting that the interaction between borreliae and the cells of the first-line defence might differ from that of other bacteria. it has been reported that human neutrophils phagocytose motile borreliae through an unconventional mechanism (tube phagocytosis) which ... | 2006 | 17049082 |
| detection of borrelia garinii, borrelia tanukii and borrelia sp. closely related to borrelia valaisiana in ixodes ticks removed from dogs and cats in japan. | ticks removed from 1136 dogs and 134 cats all over japan were examined for borrelia infection by pcr and sequencing. the 5s-23s rdna intergenic spacer of borrelia was detected from two ixodes persulcatus ticks from two dogs and two unidentified ixodes spp. from another two dogs in hokkaido, and two ixodes granulatus ticks from two cats in okinawa. additional 2 i. granulatus from the same cats also showed positive. sequence analysis of the pcr products revealed that the one from hokkaido was simi ... | 2007 | 17049744 |
| complications of pregnancy and transplacental transmission of relapsing-fever borreliosis. | relapsing-fever borreliosis caused by borrelia duttonii is a common cause of complications of pregnancy, miscarriage, and neonatal death in sub-saharan africa. we established a murine model of gestational relapsing fever infection for the study of the pathological development of these complications. we demonstrate that b. duttonii infection during pregnancy results in intrauterine growth retardation, as well as placental damage and inflammation, impaired fetal circulation, and decreased maternal ... | 2006 | 17054065 |
| alzheimer's neuroborreliosis with trans-synaptic spread of infection and neurofibrillary tangles derived from intraneuronal spirochetes. | in the realm of dementia, it is astonishing to note that neurofibrillary tangles (nft) are microscopically identical in a childhood illness (sspe) and in a dementia of late adult life (alzheimer's disease). the words "alzheimer-type" nft in peer reviewed scientific articles written by acknowledged experts underscore the striking similarities in "tangles" in two different diseases. subacute sclerosing panencephalitis (sspe) is caused by infection with atypical measles virus. alzheimer's disease h ... | 2007 | 17055667 |
| potentially misleading western blot results in lyme disease diagnosis. | 2006 | 17058717 | |
| cerebrospinal fluid-infiltrating cd4+ t cells recognize borrelia burgdorferi lysine-enriched protein domains and central nervous system autoantigens in early lyme encephalitis. | neurological manifestations of lyme disease are usually accompanied by inflammatory changes in the cerebrospinal fluid (csf) and the recruitment of activated t cells into the csf compartment. in order to characterize the phenotype and identify target antigens of csf-infiltrating t cells in early neuroborreliosis with central nervous system (cns) involvement, we combined t-cell cloning, functional testing of t-cell responses with positional scanning synthetic combinatorial peptide libraries, and ... | 2007 | 17060473 |
| antibody detection against borrelia burgdorferi in horses located in the suburban areas of monterrey, nuevo león. | the aim of the present study was to determine the presence of borrelia burgdorferi antibodies in horses from the metropolitan area of monterrey, nuevo león, méxico. blood serum was obtained from a total of 100 horses residing at different counties in the area. from each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. all sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed b ... | 2001 | 17061503 |
| the lyme vaccine: a cautionary tale. | 2007 | 17064458 | |
| lyme disease presenting as popliteal cyst in children. | lyme disease is the most common tick-borne disease in north america. our review of the literature found few reports of lyme disease presented in the orthopaedic literature. however, lyme disease presenting as a popliteal cyst, with or without rupture, is rarely reported. we present 4 cases of lyme disease that initially presented to our pediatric orthopaedic clinic for treatment of a popliteal cyst. the early diagnosis and treatment of lyme disease may help prevent the often-devastating long-ter ... | 2006 | 17065933 |
| lyme borreliosis infection as a cause of dilated cardiomyopathy. | the relatively low percent of patients affected with the cardiac form of lyme borreliosis is difficult to diagnose, especially if the disease manifests itself in ways other than atrio-ventricular blockade. the advanced stage of lyme carditis manifesting as dilated cardiomyopathy is a special case of this affliction. the authors of this report present clinical experience with an attempt to support the working hypothesis about involvement of lyme borreliosis infection in the development of dilated ... | 2006 | 17066741 |
| isolated facial diplegia: a diagnostic dilemma. | 2006 | 17069133 | |
| allozyme polymorphism of mdh and alpha-gpdh in ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks. | ixodes ricinus linnaeus (acari: ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. the allozyme variability of mdh and alpha-gpdh was detected by native polyacrylamide gel electrophoresis in i. ricinus natural populations in three localities in serbia. four alleles of mdh locus (mdh 1, mdh 2, mdh 3 and mdh x) and four alleles of alpha-gpdh locus (vs, s, f and vf) were detected. interpopulation differences in mdh and alpha-gpdh allele frequencies were statistically ... | 2006 | 17072538 |
| p38 mitogen-activated protein kinase controls nf-kappab transcriptional activation and tumor necrosis factor alpha production through rela phosphorylation mediated by mitogen- and stress-activated protein kinase 1 in response to borrelia burgdorferi antigens. | the interaction of borrelia burgdorferi, the causative agent of lyme borreliosis, with phagocytic cells induces the activation of nf-kappab and the expression of proinflammatory cytokines including tumor necrosis factor alpha (tnf-alpha). b. burgdorferi-induced tnf-alpha production is also dependent on the activation of p38 mitogen-activated protein (map) kinase. the specific contribution of these signaling pathways to the response of phagocytic cells to the spirochete and the molecular mechanis ... | 2007 | 17074860 |
| borrelia burgdorferi and anaplasma phagocytophilum coinfection. | 2006 | 17080581 | |
| [skin manifestations of lyme borreliosis]. | lyme borreliosis is the most common tick-borne disease which is caused by borrelia burgdorferi and transmitted in the poland, as well as europe, primarily by ixodes ticks. after inoculation spirochetes spreads in the skin, activate locally immune response mechanisms and cause characteristic skin lesions like erythema migrans, lymphadenosis benigna cutis, erythema migrans multiplex and acrodermatitis chronica atrophicans. the correct clinical diagnosis of skin lesions and antibiotic treatment is ... | 2006 | 17080746 |
| [outbreak of lyme borreliosis with joint manifestation--case report]. | lyme borreliosis is caused by the spirochete borrelia burgdorferi, which is transmitted into the human body by ticks. the clinical symptoms are associated with skin, joints, heart and nervous system. four groups of antibiotics are used in lyme borreliosis treatment: penicillins, cephalosporins, tetracyclines and macrolides. we present the case of lyme borreliosis with outbreak joint manifestation. | 2006 | 17080747 |
| il-10 deficiency promotes increased borrelia burgdorferi clearance predominantly through enhanced innate immune responses. | borrelia burgdorferi is capable of persistently infecting a variety of hosts despite eliciting potent innate and adaptive immune responses. preliminary studies indicated that il-10-deficient (il-10(-/-)) mice exhibit up to 10-fold greater clearance of b. burgdorferi from target tissues compared with wild-type mice, establishing il-10 as the only cytokine currently known to have such a significant effect on spirochetal clearance. to further delineate these il-10-mediated immune effects, kinetic s ... | 2006 | 17082624 |