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identification of 3-sulfinopropionyl coenzyme a (coa) desulfinases within the acyl-coa dehydrogenase superfamily.in a previous study, the essential role of 3-sulfinopropionyl coenzyme a (3sp-coa) desulfinase acyl-coa dehydrogenase (acd) in advenella mimigardefordensis strain dpn7(t) (acddpn7) during degradation of 3,3'-dithiodipropionic acid (dtdp) was elucidated. dtdp is a sulfur-containing precursor substrate for biosynthesis of polythioesters (ptes). acddpn7 showed high amino acid sequence similarity to acyl-coa dehydrogenases but was unable to catalyze a dehydrogenation reaction. hence, it was investig ...201424317404
effects of aronia melanocarpa constituents on biofilm formation of escherichia coli and bacillus cereus.many bacteria growing on surfaces form biofilms. adaptive and genetic changes of the microorganisms in this structure make them resistant to antimicrobial agents. biofilm-forming organisms on medical devices can pose serious threats to human health. thus, there is a need for novel prevention and treatment strategies. this study aimed to evaluate the ability of aronia melanocarpa extracts, subfractions and compounds to prevent biofilm formation and to inhibit bacterial growth of escherichia coli ...201324317526
a one-pot system for production of l-2-aminobutyric acid from l-threonine by l-threonine deaminase and a nadh-regeneration system based on l-leucine dehydrogenase and formate dehydrogenase.l-2-aminobutyric acid (l-aba) is an unnatural amino acid that is a key intermediate for the synthesis of several important drugs. it can be produced by transaminase or dehydrogenase from α-ketobutyric acid, which can be synthesized enzymatically from the bulk amino acid, l-threonine. deamination of l-threonine followed by a hydrogenation reaction gave almost the theoretical yield and was estimated to be more cost-effective than the established chemical process. l-threonine deaminase from escheri ...201424322776
synergy of antibacterial and antioxidant activities from crude extracts and peptides of selected plant mixture.a plant mixture containing indigenous australian plants was examined for synergistic antimicrobial activity using selected test microorganisms. this study aims to investigate antibacterial activities, antioxidant potential and the content of phenolic compounds in aqueous, ethanolic and peptide extracts of plant mixture.201324330547
characterization of an alkalophilic extracellular chitosanase from bacillus cereus gu-02.an alkalophilic extracellular chitosanase (actase) was characterized from the culture supernatant of bacillus cereus gu-02. kinetic properties of actase produced from b. cereus gu-02 after cultivation in anaerobic condition, alkaline medium (ph 10) at 37°c for 3 days were investigated. actase was found to be stable in alkaline ph range from 8 to 10. interestingly, optimum ph and temperature were estimated to be 10 and 37°c, respectively, where actase showed chitosan degrading activity (87%), whi ...201424331981
antibacterial activity of the roots, stems and leaves of alchornea floribunda.alchornea floribunda müll. arg. is used in traditional medicine across africa for the treatment of bacterial, fungal, parasitic and inflammatory disorders.201424333961
alcoholytic cleavage of polyhydroxyalkanoate chains by class iv synthases induced by endogenous and exogenous ethanol.polyhydroxyalkanoate (pha)-producing bacillus strains express class iv pha synthase, which is composed of the subunits phar and phac. recombinant escherichia coli expressing pha synthase from bacillus cereus strain yb-4 (pharcyb-4) showed an unusual reduction of the molecular weight of pha produced during the stationary phase of growth. nuclear magnetic resonance analysis of the low-molecular-weight pha revealed that its carboxy end structure was capped by ethanol, suggesting that the molecular ...201424334666
lessons learnt from a birthday party: a bacillus cereus outbreak, bari, italy, january 2012.bacillus cereus, a ubiquitous bacterium, can be isolated in various starchy food items, causing both emetic and diarrhoeal disease. the real burden of b. cereus outbreaks is actually poorly known in italy. we report a b. cereus foodborne outbreak that occurred in a pub in bari (italy) on january 22nd 2012 during a birthday party, promptly reported by the pub owner.201324334785
capillary electrophoresis of microbial aggregates.uncontrolled aggregation of bacterial cells is a significant disadvantage of electrophoretic separations. various aspects of the electrophoretic behavior of different strains of gram-positive bacillus cereus, bacillus subtilis, sarcina lutea, staphylococcus aureus(1), and micrococcus luteus bacteria and gram-negative escherichia coli bacteria were investigated in this study. our findings indicate that bacteria can be rapidly analyzed by cze with surface charge modification by calcium ions (ca(2+ ...201424338952
new derivatives of aryl-propionic acid. synthesis and biological evaluation.to design new derivatives of aryl-propionic acid with potential antibacterial and antioxidant activity.201324340542
new hydrazones with pyrazolone structure: synthesis, characterization and biological evaluation.to design new hydrazones with pyrazolone structure with improved biological properties.201324340543
bio-augmentation as a tool for improving the modified sequencing batch biofilm reactor.biological treatment of domestic sewage was accomplished in a pilot-scale modified sequencing batch biofilm reactor (sbbr) bio-augmented with consortium of 5 strains of indigenous bacteria (genus pseudomonas and bacillus). the reactor consisted of fibrous filler in the upper and ceramsite filter media in the bottom. it demonstrated to have a short hydraulic residence time (hrt) for 10 h and good quality effluent to cope with low c/n ratio domestic wastewater. the biofilm attached fibrous fillers ...201424342171
purification of a thermostable chitinase from bacillus cereus by chitin affinity and its application in microbial community changes in soil.a thermostable chitinase was purified by chitin affinity from the culture supernatant of bacillus cereus tku028 with shrimp head powder (shp) as the sole carbon/nitrogen source. tku028 chitinase was purified using a one-step affinity adsorbent system, and the molecular mass of tku028 chitinase (approximately 40 kda) was then determined using sds-page. the enzyme was stable for 60 min at temperatures below 60 °c and stable over a broad ph range of 4-9 for 60 min. in addition, the temporal changes ...201424342954
involvement of alanine racemase in germination of bacillus cereus spores lacking an intact exosporium.the l-alanine mediated germination of food isolated bacillus cereus dsa 1 spores, which lacked an intact exosporium, increased in the presence of d-cycloserine (dcs), which is an alanine racemase (alr) inhibitor, reflecting the activity of the alr enzyme, capable of converting l-alanine to the germination inhibitor d-alanine. proteomic analysis of the alkaline extracts of the spore proteins, which include exosporium and coat proteins, confirmed that alr was present in the b. cereus dsa 1 spores ...201424346000
lipids, proteins, phenolic composition, antioxidant and antibacterial activities of seeds of peanuts (arachis hypogaea l) cultivated in tunisia.fatty acid composition of peanut seed oil in four varieties cultivated in tunisia showed that linoleic (c18:2), oleic (c18:1) and palmitic (c16) acids account for more than 84% for chounfakhi and massriya and for more than 85% of the total fatty acids of trabilsia and sinya seed oil respectively. seed oil contents were significantly different (p ≤ 0.05) and did not exceed 48%. the study of total phenolics revealed that chounfakhi contained more total phenolics (2.1 mg gae/g dw), followed by the ...201324346073
characterization of amylolysin, a novel lantibiotic from bacillus amyloliquefaciens ga1.lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. they are capable to inhibit the growth of gram-positive bacteria, including listeria monocytogenes, staphylococcus aureus or bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in ...201324349428
an alkenylresorcinol derivative from hakea sericea fruits and their antimicrobial activity.hakea sericea has been introduced to portugal for ornamental purposes. the phytochemical composition and the antioxidant, antibacterial, antibiofilm and cytotoxic properties of this shrub species have been previously reported. the present work describes the bioassay-guided fractionation of the crude methanolic extract of h. sericea fruits and the isolation of 9-(3,5-dihydroxy-4-methylphenyl)nona-3(z)-enoic acid. the structure of this new compound was established by one- and two-dimensional nmr a ...201324354201
complete genome sequence of bacillus toyonensis bct-7112t, the active ingredient of the feed additive preparation toyocerin.strain bct-7112, previously identified as bacillus cereus var. toyoi, is the type strain of the species bacillus toyonensis, a novel species of the b. cereus group. the complete genome of this strain, which is the active ingredient of the feed additive preparation toyocerin, has been sequenced and annotated to reveal the genetic properties of this probiotic organism with a long history of safe use in animal nutrition.201324356843
a new bis-labdanic diterpene from the rhizomes of alpinia pahangensis.the rhizomes of alpinia pahangensis yielded a new bis-labdanic diterpene for which the name pahangensin c (1) was proposed along with twelve known analogues (2-13). the structure of 1 was elucidated via spectroscopic methods including 1d and 2d nmr techniques and lcms-it-tof analysis. compounds 2 and 12 were isolated for the first time from the genus alpinia. this is the second occurrence of compounds 2 and 12 in the zingiberaceae family. selected analogues exhibited moderate to strong inhibitor ...201324356874
genetic determinants of antimicrobial resistance in gram positive bacteria from organic foods.bacterial biocide resistance is becoming a matter of concern. in the present study, a collection of biocide-resistant, gram-positive bacteria from organic foods (including 11 isolates from genus bacillus, 25 from enterococcus and 10 from staphylococcus) were analyzed for genes associated to biocide resistance efflux pumps and antibiotic resistance. the only qac-genes detected were qaca/b (one bacillus cereus isolate) and smr (one b. cereus and two staphylococcus saprophyticus isolates). efflux p ...201424361832
antibacterial activity of 2-alkynoic fatty acids against multidrug-resistant bacteria.the first study aimed at determining the structural characteristics needed to prepare antibacterial 2-alkynoic fatty acids (2-afas) was accomplished by synthesizing several 2-afas and other analogs in 18-76% overall yields. among all the compounds tested, the 2-hexadecynoic acid (2-hda) displayed the best overall antibacterial activity against gram-positive staphylococcus aureus (mic=15.6 μg/ml), staphylococcus saprophyticus (mic=15.5 μg/ml), and bacillus cereus (mic=31.3 μg/ml), as well as agai ...201324365283
detection of emetic bacillus cereus by real-time pcr in foods.the simplex real-time pcr assays based on the taqman probe and sybr green i, which target cereulide synthetase genes (ces genes) were used for rapidly, reliably and sensitively identifying the emetic strains from among bacillus cereus strains isolated from different sources. only the emetic strains showed positive reactions to the real-time pcr assays, but all examined strains of diarrheal b. cereus, other bacillus species and other gram positive and negative bacteria gave negative results. the ...201324366630
psp: rapid identification of orthologous coding genes under positive selection across multiple closely related prokaryotic genomes.with genomic sequences of many closely related bacterial strains made available by deep sequencing, it is now possible to investigate trends in prokaryotic microevolution. positive selection is a sub-process of microevolution, in which a particular mutation is favored, causing the allele frequency to continuously shift in one direction. wide scanning of prokaryotic genomes has shown that positive selection at the molecular level is much more frequent than expected. genes with significant positiv ...201324373418
antibacterial, antifungal and antioxidant activities of honey collected from timergara (dir, pakistan).in this study honeys of acacia modesta, prunus persica, zizyphus sativa and isodon rogosus plants were tested against two gram-positive bacterial strains (staphylococcus aureus and bacillus cereus), two gram-negative bacterial strains (klebsilla pneumonia and escherichia coli) and two fungal strains (alternaria alternata and trichoderma harzianum) through agar well diffusion method. the tested honeys showed high antimicrobial activities to the tested bacterial and fungal strains. all the tested ...201424374434
haplophytin b from maclurodendron porteri.an alkaloid from maclurodendron porteri has been isolated and characterized. extraction process was conducted by acid-base extraction method followed by column chromatography. the structure was established by nuclear magnetic resonance spectroscopy and mass spectrometry. the compound was identified as haplophytin b which occurs commonly in the rutaceae family. however, this is the first time this alkaloid was isolated and reported from the species. the compound showed no inhibition against staph ...201424374446
impact of ag and al₂o₃ nanoparticles on soil organisms: in vitro and soil experiments.in vitro analyses were conducted to assess the impact of al2o3 and ag nanoparticles on two common soil bacteria, bacillus cereus and pseudomonas stutzeri. al2o3 nanoparticles did not show significant toxicity at any dose or time assayed, whereas exposure to 5 mg l(-1) ag nanoparticles for 48 h caused bactericidal effects. moreover, alterations at the morphological level were observed by transmission electron microscopy (tem); ag but not al2o3 nanoparticles evoked the entrance of b. cereus cells ...201424374587
antimicrobial effectiveness of silicone oil, heavy silicone oil and perfluorodecaline against bacillus cereus.the purpose of this work is to study the effectiveness of silicone oil, heavy silicone oil and perfluorodecaline against bacillus cereus. for this we tested the antimicrobial activities of three intraocularly used liquids--silicone oil (siluron 1000), heavy silicone oil (densiron-68) and perfluorodecaline (f-decalin)--against b. cereus (nctc 9946). the antimicrobial effects of silicone oil, heavy silicone oil and perfluorodecaline were determined by the growing capability of the microorganism. w ...201424375026
spore germination mediated by bacillus megaterium qm b1551 slel and ypeb.previous work demonstrated that bacillus megaterium qm b1551 spores that are null for the sleb and cwlj genes, which encode cortex-lytic enzymes (cles), either of which is required for efficient cortex hydrolysis in bacillus spores, could germinate efficiently when complemented with a plasmid-borne copy of ypeb plus the nonlytic portion of sleb encoding the n-terminal domain of sleb (sleb(n)). the current study demonstrates that the defective germination phenotype of b. megaterium sleb cwlj spor ...201424375103
in-vitro anticancer and antimicrobial activities of plga/silver nanofiber composites prepared by electrospinning.in the present work, a series of 0, 1 and 7 wt% silver nano-particles (ag nps) incorporated poly lactic-co-glycolic acid (plga) nano-fibers were synthesized by the electrospinning process. the plga/ag nano-fibers sheets were characterized using sem, tem and dsc analyses. the three synthesized plga/silver nano-fiber composites were screened for anticancer activity against liver cancer cell line using mtt and ldh assays. the anticancer activity of plga nano-fibers showed a remarkable improvement d ...201424375170
synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications.silver has long been advocated as an effective antimicrobial. however, toxicity issues with silver have led to limited use of silver in nanoform, especially for food preservation. with the aim of exploring combinatorial options that could increase the antibacterial potency of silver nanoparticles and reduce the effective dosage of silver, we evaluated the extent of synergy that a combination of silver nanoparticles and an essential oil representative (cinnamaldehyde) could offer. a battery of gr ...201324376352
synthesis of novel 2-(substituted amino)alkylthiopyrimidin-4(3h)-ones as potential antimicrobial agents.5-alkyl-6-(substituted benzyl)-2-thiouracils 3a,c were reacted with (2-chloroethyl) diethylamine hydrochloride to afford the corresponding 2-(2-diethylamino)ethylthiopyrimidin- 4(3h)-ones 4a,b. reaction of 3a-c with n-(2-chloroethyl)pyrrolidine hydrochloride and/or n-(2-chloroethyl)piperidine hydrochloride gave the corresponding 2-[2-(pyrrolidin-1-yl)ethyl]-thiopyrimidin-4(3h)-ones 5a-c and 2-[2-(piperidin-1-yl)ethyl]thiopyrimidin-4(3h)-ones 6a,b, respectively. treatment of 3a-d with n-(2-chloro ...201324378967
identification and validation of specific markers of bacillus anthracis spores by proteomics and genomics approaches.bacillus anthracis is the causative bacteria of anthrax, an acute and often fatal disease in humans. the infectious agent, the spore, represents a real bioterrorism threat and its specific identification is crucial. however, because of the high genomic relatedness within the bacillus cereus group, it is still a real challenge to identify b. anthracis spores confidently. mass spectrometry-based tools represent a powerful approach to the efficient discovery and identification of such protein marke ...201424379445
antibacterial activity of different natural honeys from transylvania, romania.honey is used in food industry and medicine due to its nutritive, therapeutic and dietetic qualities. the microbiological characteristics of 10 unpasteurized honey samples of known origin, collected from transylvania beekeepers (romania) were determined. the antibacterial activity of these types of honey against escherichia coli, staphylococcus aureus, staphylococcus epidermidis, salmonella enteritidis, salmonella anatum, salmonella choleraesuis, bacillus cereus, bacillus subtilis subsp. spizize ...201424380618
differential regulation and activity against oxidative stress of dps proteins in bacillus cereus.in this study, the sequence similarity, structure, ferroxidase activity and efficacy in antagonizing oxidative stress of three dps-like proteins, dps1, dps2 and dps3, encoded by bacillus cereus were comparatively analyzed. the three dps-like proteins are homologous to other bacterial dps proteins that exhibit ferroxidase activity. both dps1 and dps2 have a typical dps spherical structure, but dps3 has a unique filamentous structure. several dps mutant strains were generated to investigate the fu ...201324383075
draft genome sequence of bacillus cereus lct-bc25, isolated from space flight.bacillus cereus strain lct-bc25, which was carried by the shenzhou viii spacecraft, traveled in space for about 398 h. to investigate the response of b. cereus to space environments, we determined the genome sequence of b. cereus strain lct-bc25, which was isolated after space flight.201424385570
dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of cr6+ from real tannery effluent employing indigenous bacillus cereus isolate.a native bacillus cereus isolate has been employed, for the first time, for simultaneous decolorization, dechlorination of chloroorganics, and cr(6+) remediation from the real tannery effluent. most of the physicochemical variables in 3:1 diluted effluent were well above the standard prescribed limits, which decreased substantially upon microbial treatment. the extent of bioremediation was better in diluted (3:1) as compared to undiluted effluent supplemented with nutrients and augmented with b. ...201424390198
antimicrobial activity of traditional medicinal plants from ankober district, north shewa zone, amhara region, ethiopia.traditional medicinal plants have long been used in ethiopia to treat human and livestock ailments. despite a well-documented rich tradition of medicinal plant use in the country, their direct antimicrobial effects are still poorly known.201424392738
trifolium pratense l. as a potential natural antioxidant.the essential oils of three different growth stages of trifolium pratense l. (tp1, tp2 and tp3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. the highest content of volatile compounds was found in the essential oil sample tp1, where terpenes such as β-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. the antioxidant activity was determined by f ...201424402202
effects of methanol extract of wedelia chinensis osbeck (asteraceae) leaves against pathogenic bacteria with emphasise on bacillus cereus.the antibacterial activity of the methanol extract of wedelia chinensis leave was studied and tested against three pathogenic gram positive bacteria (bacillus cereus, b. subtilis and stapylococcus aureus) and three pathogenic gram negative bacteria (escherichia coli, proteus rettgeri and pseudomonas aeruginosa) by the disk diffusion assay and broth dilution methods. the extract exhibited favourable antibacterial activity against the bacterial cells but was more potent against gram positive bacte ...201324403653
effect of temperatures on the growth, toxin production, and heat resistance of bacillus cereus in cooked rice.bacillus cereus is capable of producing enterotoxin and emetic toxin, and bacillus foodborne illnesses occur due to the consumption of food contaminated with endospores. the objectives of this study were to investigate the growth and toxin production of b. cereus in cooked rice and to determine the effect of temperature on toxin destruction. cooked rice inoculated with b. cereus was stored at 15, 25, 35, and 45°c or treated at 80, 90, and 100°c. the results indicated that emetic toxin was produc ...201424404779
genome sequence of bacillus cereus strain lct-bc235, carried by the shenzhou viii spacecraft.in order to explore the effect of space environments on bacillus cereus, we determined the draft genome sequence of a b. cereus strain, lct-bc235, which was isolated after space flight.201424407625
exoproteome analysis of a novel strain of bacillus cereus implicated in disease resembling cutaneous anthrax.bacillus cereus belongs to b. cereus sensu lato group, shared by six other related species including bacillus anthracis. b. anthracis is the causative agent for serious illness affecting a wide range of animals as well as humans and is a category a biological and toxin warfare (btw) agent. recent studies indicate that a bacillus species other than b. anthracis can cause anthrax-like disease and role of anthrax virulence plasmids (pxo1 and pxo2) on the pathogenicity of b. cereus has been document ...201424412723
distribution of toxigenic bacillus cereus in rice samples marketed in hong kong.eleven of 16 samples of rice on sale in rice shops and supermarkets in hong kong contained bacillus cereus. although b. cereus counts did not exceed 100 bacteria/g in most of the positive samples, a sample of thai red rice and a poor quality rice originating from china contained between 300 to 1000 cells/g and 10(4) to 2×10(5) cells/g, respectively. nine strains produced an enterotoxin responsible for the diarrhoeal-type b. cereus food poisoning and seven of these strains also produced a haemoly ...199524415029
[clinical characteristics and resistance of wound surface infections causing by bacillus cereus in the department of orthopaedics].to study clinical characteristics and resistance of wound surface infections, so as to guide clinical diagnosis and rational administration.201324416908
biosynthesis and characterization of mercury sulphide nanoparticles produced by bacillus cereus mrs-1.mercury is a highly toxic heavy metal accumulated in the environment, which can be detoxified by reducing hg2+ to non toxic form. bacteria resistant to toxic metals and capable of converting them into non toxic forms have a direct application in the bioremediation of contaminated sites. in this study, mercury resistant strain bacillus cereus mrs-1 was isolated from electroplating industrial effluent. this strain exhibited the ability to convert mercury into extracellular sulphide nanoparticles o ...201324416933
inhibition of bacillus spores by combinations of heat, potassium sorbate, nacl and ph.viability of spores of bacillus cereus was totally inhibited at 85°c over 30 min by adding 0.4% (w/v) potassium sorbate with 6% (w/v) nacl at ph 4.5. viability of b. stearothermophilus spores was totally inhibited at 95°c for 45 min in a buffer at ph 4.2 containing 0.8% (w/v) potassium sorbate and 8% (w/v) nacl. a synergistic inhibitory effect was demonstrated in some of the combinations. the inhibition may be due to interference with the heat-resistance apparatus of the spores.199424421139
ph and acidity in lactic-fermenting cereal gruels: effects on viability of enteropathogenic microorganisms.survival of bacillus cereus, campylobacter jejuni, enterotoxigenic escherichia coli, salmonella typhimurium and shigella flexneri during lactic acid fermentation of cereal gruels prepared from low-tannin (white) and high-tannin (red) sorghum varieties was studied. a previously fermented gruel (starter culture, sc) recycled daily or stored for 7, 14 or 28 days, germinated cereal flour (power flour, pf), or a combination of pf and sc (pf+sc) were used as starters. at 24 h, the ph of all gruels wit ...199424421191
inhibited growth of common enteropathogenic bacteria in lactic-fermented cereal gruels.a natural lactic fermentation of mixtures of water and whole flour of either maize or high-tannin sorghum was obtained either before or after cooking to a weaning gruel: the preparations had a final ph of about 3.8 (range 3.67 to 4.00) and a ratio of lactic acid to acetic acid of 9∶1 (w/w). the growth of added (about 10(7) c.f.u./g gruel) gram-negative intestinal pathogenic bacteria, enterotoxigenicescherichia coli, campylobacter jejuni, shigella flexneri andsalmonella typhimurium, was strongly ...199224425608
impact of lactococcus lactis spp. lactis bio adhesion on pathogenic bacillus cereus biofilm on silicone flowing system.bacillus cereus is a food pathogen that can attach on most of the surfaces and form biofilms, which facilitate the persistence and resistance toward antimicrobials. the aims of this study were (i) to characterize the structural dynamics of b. cereus sessile growth in two nutritional environments (with or without a nutrient flow), and (ii) to evaluate the impact of bio adhesion of lactococcus lactis on b. cereus biofilm. significantly greater biofilm volume and thickness were observed under dynam ...201324426121
microbial treatment for prevention and removal of paraffin deposition on the walls of crude pipelines.two bacterial strains, paraffin removal strain and biosurfactant-producing strain, named bhj-1 and qfl-1, were isolated from oil production wells in daqing oilfield of china. they were subsequently identified as bacillus cereus qau68 and bacillus subtilis xccx, respectively. as an indicator of the degradation paraffin, the inoculum concentration of bhj-1 and qfl-1 were added in different proportions, the optimum proportion was 5:2. in this proportion the degradation rate of paraffin could reach ...201324426154
in vitro characterization of a recombinant ahl-lactonase from bacillus cereus isolated from a striped catfish (pangasianodon hypophthalmus) pond.aiia gene encoding ahl-lactonase was isolated from bacillus cereus strain n26.2, originating from a striped catfish (pangasianodon hypophthalmus) pond in vietnam. this gene, abbreviated as aiia(n26.2), was cloned and expressed in a competent escherichia coli strain bl21(de3)plyss. the resulting protein, abbreviated as aiian26.2, was highly active in the ph range of 6-8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °c or for 3 days at 20 °c. these properties of aiian ...201324426155
impact of donor arm cleaning with different aseptic solutions for prevention of contamination in blood bags.transfusion associated sepsis cases are encountered occasionally and bacterial transmission remains the major cause. the goal of our study was to compare the efficacy of disinfectants in phlebotomy site preparation. after selection of donor the antecubital fossa area of the arm was disinfected with different types of disinfectants namely sprit (70% isopropyl alcohol), povidone iodine (0.5% w/v available iodine in distilled water), savlon (1.5% v/v chlorhexidine gluconate solution and 3.0% cetrim ...201324426327
synthesis, characterization and biological studies of substituted quinozoline-4-(3h)-ones containing diazepine moiety.the synthesis and characterization of new series of 1,4-benzodiazepine derivatives have been presented. the structures were confirmed by elemental analyses, ir spectral, (1)h nmr spectral and mass spectral data. all the compounds were screened for in vitro antimicrobial and anthelmintic activities. the antibacterial activity was tested against staphylococcus aureus (gram positive), bacillus cereus (gram positive), escherichia coli (gram negative) and pseudomonas aeruginosa (gram negative). the a ...201424438669
isolation of bacillus cytotoxicus from various commercial potato products.bacillus (b.) cytotoxicus is a newly described thermotolerant member of the bacillus cereus group. this potential foodborne pathogen had so far only been isolated from vegetable products, including mashed potatoes. here we report the detection of b. cytotoxicus in a variety of potato products taken on retail level or from catering establishments (n=151). identification of isolates as b. cytotoxicus was performed after enrichment at 50°c, followed by differentiation using fourier transform-infrar ...201424440535
hydrothermal synthesis of copper based nanoparticles: antimicrobial screening and interaction with dna.copper based nanoparticles (cu-based nps) of different compositions and sizes have been hydrothermally synthesized by varying the reaction time in the presence of the biocompatible surfactants polyoxyethylene (20) sorbitan laurate (tween 20) and polyethylene glycol 8000 (peg 8000). effective control of the above synthetic parameters gave rise to cu, cu2o and cu/cu2o nps of 10-44 nm. the antibacterial activity of the nps was screened against gram-positive (bacillus subtilis, bacillus cereus, stap ...201424441110
surfactin restores and enhances swarming motility under heavy metal stress.the present work reports the importance of lipopeptide biosurfactant on swarming motility of multi-metal resistant (mmr) bacterium under heavy metal stress. the mmr bacteria strain cm100b, identified as bacillus cereus, was isolated from the coal mine sample. the strain was able to grow and reduce several metals namely cd(2+), co(2+), cu(2+), ni(2+), mn(2+) and pb(2+) ions which are common environmental pollutants. presence of toxic heavy metal ions in the swarming medium significantly altered t ...201424441179
purification of chitinase/chitosanase from bacillus cereus and discovery of an enzyme inhibitor.a chitinase and a chitosanase were induced from a squid pen powder (spp)-containing medium of bacillus cereus tku030 and purified by precipitation with ammonium sulphate and combined column chromatography. the purified chitinase and chitosanase exhibited optimum activity at 60 °c, ph 5-6 and 40 °c, ph 4, respectively. the chitinase and chitosanase were stable at 25-60 °c, ph 4-7 and 25-50 °c, ph 3-7, respectively. the chitinase and chitosanase showed the highest activity toward β-chitin and 60% ...201424444885
brown seaweed (saccharina japonica) as an edible natural delivery matrix for allyl isothiocyanate inhibiting food-borne bacteria.the edible, brown seaweed saccharina japonica was prepared as powder in the size range 500-900 μm for the desorption release of allyl isothiocyanate (aitc). powders were used as raw (containing lipids) and as de-oiled, where the lipid was removed. in general, de-oiled powders adsorbed larger masses of aitc after vapour or solution contact. mass adsorbed due to solution contact exceeded vapour contact. larger particles adsorbed more than smaller particles. no chemical bonding between aitc and the ...201424444900
nisin a extends the shelf life of high-fat chilled dairy dessert, a milk-based pudding.the aims of this study were to evaluate the effectiveness of nisin a to control the growth of spore-forming bacteria, bacillus and paenibacillus, in chilled high-fat, milk pudding and to reduce heat treatment to improve aroma and flavour.201424450783
antimicrobial activity, growth inhibition of human tumour cell lines, and phytochemical characterization of the hydromethanolic extract obtained from sapindus saponaria l. aerial parts.the hydromethanolic extract of sapindus saponaria l. aerial parts was investigated for antimicrobial activity (against several gram-positive and gram-negative bacteria and fungi) and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. the evaluated extract was further characterized in terms of phytochemicals using uv, (1)h-nmr, (13)c-nmr, and ms spectroscopic tools. the extract has shown a significant antimicrobial activity on all tested bacterial and ...201324455713
genome sequences of three novel bacillus cereus bacteriophages.the bacillus cereus group is an assemblage of highly related firmicute bacteria that cause a variety of diseases in animals, including insects and humans. we announce three high-quality, complete genome sequences of bacteriophages we isolated from soil samples taken at the bases of fruit trees in utah county, utah. while two of the phages (shanette and jl) are highly related myoviruses, the bacteriophage basilisk is a siphovirus.201424459255
the regulated synthesis of a bacillus anthracis spore coat protein that affects spore surface properties.examine the regulation of a spore coat protein and the effects on spore properties.201424460572
detection of foodborne pathogens and analysis of aflatoxin levels in home-made doenjang samples.doenjang is a traditional korean fermented soybean product that provides a major source of protein. in this study, a total of 18 different home-made doenjang samples were examined for the presence of foodborne pathogens and the total aflatoxin levels. using an enzyme-linked immunosorbent assay to assess microbial quality and potential public health risk, we showed that total coliform levels in the doenjang samples ranged from 0 to 4.43±2.32 ×10(6) cfu/g, and the maximum limit of bacillus cereus ...201224471080
novel giant siphovirus from bacillus anthracis features unusual genome characteristics.here we present vb_bans-tsamsa, a novel temperate phage isolated from bacillus anthracis, the agent responsible for anthrax infections in wildlife, livestock and humans. tsamsa phage is a giant siphovirus (order caudovirales), featuring a long, flexible and non-contractile tail of 440 nm (not including baseplate structure) and an isometric head of 82 nm in diameter. we induced tsamsa phage in samples from two different carcass sites in etosha national park, namibia. the tsamsa phage genome is th ...201424475065
spectroscopy and solvatochromism studies along with antioxidant and antibacterial activities investigation of azo-azomethine compounds 2-(2-hydroxyphenylimino)methyl)-4-phenyldiazenyl)phenol.the azo-azomethine compounds 2-(2-hydroxyphenylimino)methyl)-4-phenyldiazenyl)phenol (2a-d) were prepared from the reaction of 2-aminophenol with 2-hydroxy-5-(aryldiazenyl)benzaldehyde (1a-d). the structures of all compounds were then characterized by elemental analysis, mass, infrared, uv-vis, (1)h and (13)c nmr spectroscopy. the electronic absorption spectra indicated enol-keto tautomeric and positive solvatochromism in compounds (2a-d), which is dependent on the substitution, nature of solven ...201424480116
expression, purification, and solubility optimization of peptidyl-trna hydrolase 1 from bacillus cereus.peptidyl-trna hydrolase 1 cleaves the ester bond of peptidyl-trna thereby recycling peptidyl-trnas generated from premature termination of translation and expression of minigenes and short orfs. bacterial pth1 is essential, highly conserved, and has no essential eukaryotic homolog making it a good target for antibacterial development. herein we describe the cloning of pth1 gene from bacillus cereus as an n-terminal hexahistidine fusion protein. solubility was optimized for overexpression in esch ...201424480186
global overview of the risk linked to the bacillus cereus group in the egg product industry: identification of food safety and food spoilage markers.to evaluate the food safety and spoilage risks associated with psychrotrophic bacillus cereus group bacteria for the egg product industry and to search for relevant risk markers.201424484429
efficacy of commercial natural antimicrobials alone and in combinations against pathogenic and spoilage microorganisms.microbial control strategies are needed in the food industry to prevent foodborne illnesses and outbreaks and prolong product shelf life. the aim of this study was to investigate and compare the efficacy of the commercial natural antimicrobials white mustard essential oil (wmeo), citrus flavonoid and acid blend (cfab), olive extract (oe), nisaplin (a compound containing nisin), and lauric arginate (lae) alone and in combinations against foodborne pathogens and spoilage microorganisms. mics of in ...201424490921
multiplex pcr (polymerase chain reaction) assay for detection of e. coli o157:h7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon).the coastal aquaculture mainly shrimps constitute major export sector in bangladesh and is increasingly shaped by international trade conditions and by national responses to those stringent quality and safety standards. pcr based validated methods for detection of major bacterial pathogens in shrimp might be very useful tool for ensuring quality and safety standards of exportable shrimps. the objective of this study was to evaluate overall performance (sensitivity and specificity) of the multipl ...201324498789
vacuum distillation residue upgrading by an indigenous bacillus cereus.biological processing of heavy fractions of crude oils offers less severe process conditions and higher selectivity for refining. biochemical processes are expected to be low demand energy processes and certainly ecofriendly.201324499629
inactivation and ultrastructure analysis of bacillus spp. and clostridium perfringens spores.bacterial endospores are resistant to many environmental factors from temperature extremes to ultraviolet irradiation and are generally more difficult to inactivate or kill than vegetative bacterial cells. it is often considered necessary to treat spores or samples containing spores with chemical fixative solutions for prolonged periods of time (e.g., 1-21 days) to achieve fixation/inactivation to enable electron microscopy (em) examination outside of containment laboratories. prolonged exposure ...201424503289
food-borne pathogens of animal origin-diagnosis, prevention, control and their zoonotic significance: a review.the term food borne diseases or food-borne illnesses or more commonly food poisoning are used to denote gastrointestinal complications that occur following recent consumption of a particular food or drink. millions of people suffer worldwide every year and the situation is quiet grave in developing nations creating social and economic strain. the food borne pathogens include various bacteria viz., salmonella, campylobacter, escherichia coli, listeria monocytogenes, yersinia enterocolitica, staph ...201324506006
antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract.the use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability. therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films. as a natural antimicrobial agent, grapefruit seed extract (gse) has been incorporated into agar to prepare antimicrobial packaging film. the films with different concentrations of gse were pr ...201424507339
the caskr two-component system is required for the growth of mesophilic and psychrotolerant bacillus cereus strains at low temperatures.the different strains of bacillus cereus can grow at temperatures covering a very diverse range. some b. cereus strains can grow in chilled food and consequently cause food poisoning. we have identified a new sensor/regulator mechanism involved in low-temperature b. cereus growth. construction of a mutant of this two-component system enabled us to show that this system, called caskr, is required for growth at the minimal temperature (tmin). caskr was also involved in optimal cold growth above tm ...201424509924
rapid homogenous time-resolved fluorescence (htrf) immunoassay for anthrax detection.infection with bacillus anthracsis spores induces an acute anthrax disease that can cause casualties and death in untreated cases. thus rapid diagnosis of anthrax at early stage of the disease is essential to allow an effective treatment. here we present the development of rapid and sensitive homogenous time-resolved fluorescence (htrf) immunoassays based on the energy transfer process of europium cryptate (euk) donor to alexafluor647 acceptor. the energy transfer process is limited to d < 10 nm ...201424515915
influence of phenolic compounds of kangra tea [camellia sinensis (l) o kuntze] on bacterial pathogens and indigenous bacterial probiotics of western himalayas.phenolic compounds of nutraceutical importance viz., catechins (c), (-)-epicatechin (ec), (-)-epigallocatechin (egc), (-)-epigallocatechin-3-gallate (egcg) and (-)-epicatechin-3-gallate (ecg) were estimated in fresh green tea shoots of camellia sinensis (l) o kuntze cultivar. the total polyphenols and total catechins were in the range of 219.90 to 317.81 and 140.83 to 271.39 g/kg, respectively in monthly samples of tea. the values of c, ec, egc, egcg and ecg in tea powders as analyzed through hi ...201324516437
influence of probiotic bacterium bacillus cereus isolated from the gut of wild shrimp penaeus monodon in turn as a potent growth promoter and immune enhancer in p. monodon.a probiotic bacterium isolated from the gut of wild shrimp penaeus monodon rendered maximum antagonistic activity against shrimp pathogens and was capable of producing extracellular enzymes. the probiotic bacterium was identified as bacillus cereus through 16s rrna sequencing. the lyophilized b. cereus was supplemented with shrimp basal diet at four different concentrations (0.1–0.4%/100 g feed) in d1–d4 diets. the viability of probiotic bacterium in the test diets was evaluated during the study ...201424516873
antibacterial potency of housefly larvae extract from sewage sludge through bioconversion.use of the fly to convert sewage sludge into nutrient-rich soil conditioner and amendment is an attractive approach for sludge bioconversion. during this process, fecal coliforms, an indicating pathogen, in sludge were reduced to 5.3 x 10(2) most probable number/g dry solid from initial 3.32 x 10(6) mpn/g dry solid. it was also found that the extract of larvae grown in sludge during bioconversion have an observable inhibitory effect against bacteria compared to larvae grown in wheat bran as meas ...201324520734
cerecidins, novel lantibiotics from bacillus cereus with potent antimicrobial activity.lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are widely produced by gram-positive bacteria, including many species of the bacillus group. in the present study, one novel gene cluster coding lantibiotic cerecidins was unveiled in bacillus cereus strain as 1.1846 through genomic mining and pcr screening. the designated cer locus is different from that of conventional class ii lantibiotics in that it included seven tandem precursor cera genes ...201424532070
colloidal polyaniline dispersions: antibacterial activity, cytotoxicity and neutrophil oxidative burst.polyaniline colloids rank among promising application forms of this conducting polymer. cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. the antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for bacillus cereus and escherichia coli, with a minimum inhibitory concentration of 3,500 μg ml(-1). the data recorded on human keratinocyte (hac ...201424534430
antimicrobial activity of acid-hydrolyzed citrus unshiu peel extract in milk.citrus fruit (citrus unshiu) peels were extracted with hot water and then acid-hydrolyzed using hydrochloric acid. antimicrobial activities of acid-hydrolyzed citrus unshiu peel extract were evaluated against pathogenic bacteria, including bacillus cereus, staphylococcus aureus, and listeria monocytogenes. antilisterial effect was also determined by adding extracts at 1, 2, and 4% to whole, low-fat, and skim milk. the cell numbers of b. cereus, staph. aureus, and l. monocytogenes cultures treate ...201424534507
penicillin sensitivity as compared with nucleic acid phosphorus content of virulent and avirulent strains of bacillus anthracis and bacillus cereus. 195924545148
iron acquisition in bacillus cereus: the roles of ilsa and bacillibactin in exogenous ferritin iron mobilization.in host-pathogen interactions, the struggle for iron may have major consequences on the outcome of the disease. to overcome the low solubility and bio-availability of iron, bacteria have evolved multiple systems to acquire iron from various sources such as heme, hemoglobin and ferritin. the molecular basis of iron acquisition from heme and hemoglobin have been extensively studied; however, very little is known about iron acquisition from host ferritin, a 24-mer nanocage protein able to store tho ...201424550730
bioaccumulation characterization of cadmium by growing bacillus cereus rc-1 and its mechanism.in an effort to explore the protective mechanism of growing bacillus cereus rc-1 against the toxicity of different cd(ii) concentrations, bacterial growth, cadmium consumption, surface interactions and intra- and extra-cellular cd(ii) contents were examined. cellular morphology and growth were evidently affected by the initial metal concentrations above 20 mg l(-1), according to the analysis of sem, afm, tem and uv spectrophotometer. surface complexation and electrostatic attraction played an im ...201424560281
provenance in bioinformatics workflows.in this work, we used the prov-dm model to manage data provenance in workflows of genome projects. this provenance model allows the storage of details of one workflow execution, e.g., raw and produced data and computational tools, their versions and parameters. using this model, biologists can access details of one particular execution of a workflow, compare results produced by different executions, and plan new experiments more efficiently. in addition to this, a provenance simulator was create ...201324564294
bacillus cereus certhrax adp-ribosylates vinculin to disrupt focal adhesion complexes and cell adhesion.bacillus cereus is often associated with mild to moderate gastroenteritis; however, some recent isolates cause inhalational anthrax-like diseases and death. these potential emerging human pathogens express multiple virulence factors. b. cereus strain g9241 expresses anthrax toxin, several polysaccharide capsules, and the novel adp-ribosyltransferase, certhrax. in this study, we show that certhrax adp-ribosylates arg-433 of vinculin, a protein that coordinates actin cytoskeleton and extracellular ...201424573681
diffusible signal factor (dsf) quorum sensing signal and structurally related molecules enhance the antimicrobial efficacy of antibiotics against some bacterial pathogens.extensive use of antibiotics has fostered the emergence of superbugs that are resistant to multidrugs, which becomes a great healthcare and public concern. previous studies showed that quorum sensing signal dsf (diffusible signal factor) not only modulates bacterial antibiotic resistance through intraspecies signaling, but also affects bacterial antibiotic tolerance through interspecies communication. these findings motivate us to exploit the possibility of using dsf and its structurally related ...201424575808
seasonal trend and clinical presentation of bacillus cereus bloodstream infection: association with summer and indwelling catheter.bacillus cereus, an opportunistic pathogen, can cause fatal infection. however, b. cereus bloodstream infections (bsis) have not been well characterised. from 2008 to 2013, b. cereus isolates from all of the specimens and patients with b. cereus bsis were identified. environmental samples were collected to detect b. cereus contamination. we also characterised the clinical presentation of b. cereus bsi through analyses of risk factors for bsi and mortality. a total of 217 clinical b. cereus isola ...201424584718
soy whey based medium for optimized phytase activity in saccharomyces cerevisiae mtcc 5421 and α-d-galactosidase and antibacterial activities in lactobacillus plantarum mtcc 5422 by response surface methodology.soy whey based growth medium for yeast enabled the native isolate of saccharomyces cerevisiae mtcc 5421 to produce 198 u/ml of phytase activity against that of 135 u/ml in potato dextrose broth. in the same medium for lactic acid bacteria, the isolate of lactobacillus plantarum mtcc 5422 was able to elaborate α-d-galactosidase activity of 10.6 u/ml in comparison to 6 u/ml in lactobacillus mrs broth. using this formulated medium, a central composite experimental design based on 5 variables of 3 f ...201424587527
characterization and genome analysis of the bacillus cereus-infecting bacteriophages bps10c and bps13.due to the emergence of antibiotic-resistant strains, bacteriophages are considered to be an alternative approach for the control of pathogens. in this study, the bacteriophages bps10c and bps13 were isolated and characterized to investigate their ability to control food-borne pathogenic bacillus cereus. phage bps13 exhibited slightly higher host lysis activity compared with phage bps10c. in addition, phage bps13 exhibited greater stability under various ph and temperature conditions. to extend ...201424595561
de-hairing protease production by an isolated bacillus cereus strain at under solid-state fermentation using cow dung: biosynthesis and properties.agro-industrial residues and cow dung were used as the substrate for the production of alkaline protease by bacillus cereus strain at. the bacterial strain bacillus cereus strain at produced a high level of protease using cow dung substrate (4813 ± 62 u g(-1)). physiological fermentation factors such as the incubation time (72 h), the ph (9), the moisture content (120%), and the inoculum level (6%) played a vital role in the enzyme bioprocess. the enzyme production improved with the supplementat ...201424596497
crystal and solution structure analysis of fhud2 from staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-b.iron acquisition is a central process for virtually all organisms. in staphylococcus aureus, fhud2 is a lipoprotein that is a high-affinity receptor for iron-bound hydroxamate siderophores. in this study, fhud2 was crystallized bound to ferrioxamine-b (fxb), and also in its ligand-free state; the latter structures are the first for hydroxamate-binding receptors within this protein family. the structure of the fhud2-fxb conformation shows that residues w197 and r199 from the c-terminal domain don ...201424606332
quantification of the impact of single and multiple mild stresses on outgrowth heterogeneity of bacillus cereus spores.outgrowth heterogeneity of bacterial spore populations complicates both prediction and efficient control of spore outgrowth. in this study, the impact of mild preservation stresses on outgrowth of bacillus cereus atcc 14579 spores was quantified during the first stages of outgrowth. heterogeneity in outgrowth of heat-treated (90°c for 10 min) and non-heat-treated germinated single spores to the maximum micro-colony stage of 256 cells was assessed by direct imaging on anopore strips, placed on bh ...201424607860
isolation and characterization of chitinase-producing bacillus and paenibacillus strains from salted and fermented shrimp, acetes japonicus.chitinases catalyze the conversion of chitin and are produced by a wide range of bacteria. the biological applications of these enzymes have been exploited in food and pharmaceutical industries. we isolated 2 halophilic chitinase-producing novel strains of bacteria-sch-1 and sch-2 from saeu-jeot, a traditional korean salted and fermented food made with shrimp (acetes japonicus). the isolated strains- sch-1 and sch-2 were gram-positive, rod-shaped, endospore-forming facultative anaerobes, with st ...201424611959
[bacteriological quality and toxigenic bacillus cereus detection in cooked white rice sold at the metropolitan area of san josé, costa rica].the wide use of rice is one of the factors that favors its implication in food borne diseases, and one of the most important pathogens associated to it is bacillus cereus. the aim of this work was to evaluate the microbiological quality of 50 samples of white cooked rice sold in restaurants at the metropolitan area of san josé, costa rica, including the determination of the total aerobic plate count, the most probable number of total and fecal coliforms and escherichia coli. mpn of bacillus cere ...201224617031
comparative proteomic analysis of experimental evolution of the bacillus cereus-ketogulonicigenium vulgare co-culture.the microbial co-culture system composing of ketogulonicigenium vulgare and bacillus cereus was widely adopted in industry for the production of 2-keto-gulonic acid (2-kga), the precursor of vitamin c. we found serial subcultivation of the co-culture could enhance the yield of 2-kga by 16% in comparison to that of the ancestral co-culture. to elucidate the evolutionary dynamics and interaction mechanisms of the two microbes, we performed itraq-based quantitative proteomic analyses of the pure cu ...201424619085
association between neutropenia and brain abscess due to bacillus cereus bacteremia in patients with hematological malignancies. 201424625329
diversity of pulsed-field gel electrophoresis patterns of cereulide-producing isolates of bacillus cereus and bacillus weihenstephanensis.bacillus cereus is an important foodborne pathogen causing diarrhoea, emesis and in, rare cases, lethal poisonings. the emetic syndrome is caused by cereulide, a heat-stable toxin. originally considered as a rather homogenous group, the emetic strains have since been shown to display some diversity, including the existence of two clusters of mesophilic b. cereus and psychrotolerant b. weihenstephanensis. using pulsed-field gel electrophoresis (pfge) analysis, this research aimed to better unders ...201424627989
a fullerene colloidal suspension stimulates the growth and denitrification ability of wastewater treatment sludge-derived bacteria.fullerene (c60) is a nanoparticle that has been widely studied and applied in numerous commodities. however, there are concerns regarding its potential negative impact on the environment. a fullerene colloidal suspension (nc60) is known for its property of selectively inhibiting the growth of microorganisms. in this study, using denaturing gradient gel electrophoresis fingerprinting technology, we found that fullerene altered the structure of a sludge-derived microbial community. specifically, t ...201424630252
green synthesis of silver nanoparticles using delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities.green synthesis of silver nanoparticles (agnps) using aqueous root extract of delphinium denudatum (dd) by reduction of ag(+) ions from silver nitrate solution has been investigated. the synthesized ddagnps were characterized by using uv-vis spectroscopy, x-ray diffraction (xrd), field emission scanning electron microscope (fesem) and fourier transform infrared spectroscopy (ftir). the prepared ddagnps showed maximum absorbance at 416nm and particles were polydispersed in nature, spherical in sh ...201424632157
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