Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| production of phytophthora infestans-resistant potato (solanum tuberosum) utilising ensifer adhaerens ov14. | based on the use of agrobacterium tumefaciens-mediated transformation commodity crop improvement through genetic engineering is the fastest adopted crop technology in the world (james 2010). however, the complexity of the agrobacterium patent landscape remains a challenge for non-patent holders who wish to generate novel varieties for a commercial purpose. the potential of non-agrobacterium strains (transbacter(™)) to modify a plant genome has previously been described. however, they are unlikel ... | 2011 | 21912851 |
| Flavonoid production in transgenic hop (Humulus lupulus L.) altered by PAP1/MYB75 from Arabidopsis thaliana L. | Hop is an important source of secondary metabolites, such as flavonoids. Some of these are pharmacologically active. Nevertheless, the concentration of some classes as flavonoids in wild-type plants is rather low. To enhance the production in hop, it would be interesting to modify the regulation of genes in the flavonoid biosynthetic pathway. For this purpose, the regulatory factor PAP1/AtMYB75 from Arabidopsis thaliana L. was introduced into hop plants cv. Tettnanger by Agrobacterium-mediated g ... | 2012 | 21912858 |
| genome sequence of agrobacterium tumefaciens strain f2, a bioflocculant-producing bacterium. | agrobacterium tumefaciens f2 is an efficient bioflocculant-producing bacterium. but the genes related to the metabolic pathway of bioflocculant biosynthesis in strain f2 are unknown. we present the draft genome of a. tumefaciens f2. it could provide further insight into the biosynthetic mechanism of polysaccharide-like bioflocculant in strain f2. | 2011 | 21914861 |
| Remediation of oil-based drill cuttings through a biosurfactant-based washing followed by a biodegradation treatment. | In this study, oil-based drill cuttings were washed by a rhamnolipid solution and then subjected to bioremediation in stainless steel boxes using sawdust as bulking agent. A mixed bacterial culture, mainly containing Pseudomonas, Acinetobacter, Alcaligenes, Agrobacterium, and Comamonas, was used as inoculums. Approximately 83% of organics were removed after washing under optimal conditions (liquid/solid ratio, 3:1; washing time, 20 min; stirring speed, 200 rpm; rhamnolipid concentration, 360 mg/ ... | 2011 | 21925873 |
| testing the 3'-untranslated rna regions of beet necrotic yellow vein virus and beet yellows virus as inducers of posttranscriptional gene silencing. | 2011 | 21928144 | |
| Expression of a novel antimicrobial peptide Penaeidin4-1 in creeping bentgrass (Agrostis stolonifera L.) enhances plant fungal disease resistance. | Turfgrass species are agriculturally and economically important perennial crops. Turfgrass species are highly susceptible to a wide range of fungal pathogens. Dollar spot and brown patch, two important diseases caused by fungal pathogens Sclerotinia homoecarpa and Rhizoctonia solani, respectively, are among the most severe turfgrass diseases. Currently, turf fungal disease control mainly relies on fungicide treatments, which raises many concerns for human health and the environment. Antimicrobia ... | 2011 | 21931807 |
| Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference. | Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming. Recently, transient expression of genes in grapevine leaves has been obtained by Agrobacterium tumefaciens infiltration (agroinfiltration). We th ... | 2012 | 21932028 |
| cloning a peanut resveratrol synthase gene and its expression in purple sweet potato. | a resveratrol synthase gene was cloned from the peanut plant (arachis hypogaea) by rt-pcr and was transformed into purple sweet potato (ipomoea batatas) by agrobacterium-mediated transformation. stem sections were infected with bacterial solution of od(600) = 0.4 for 20 min and then cocultured for 2 days. infected explants were cultured on ms media containing 50 mg/l kanamycin, 0.02 mg/l naa and 1 mg/l 6-ba for bud induction or containing 75 mg/l kanamycin, 1.0 mg/l naa and 0.1 mg/l 6-ba for roo ... | 2012 | 21932029 |
| A bacterial artificial chromosome library for 'Jefferson' hazelnut and identification of clones associated with eastern filbert blight resistance and pollen-stigma incompatibility. | European hazelnut (Corylus avellana L.) is the only economically important nut crop in the family Betulaceae. Because of its small genome size (~385 Mb / 1C), relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, the European hazelnut could serve as a model plant for the Betulaceae. Here we report the construction of a bacterial artificial chromosome (BAC) library for 'Jefferson' hazelnut using the cloning enzyme MboI and the vector ... | 2011 | 21936690 |
| resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in cell cultures of vitis amurensis treated with coumaric acid. | the present study examines the effect of p-coumaric acid (ca), a precursor of stilbenes and isoflavonoids, on biosynthesis of resveratrol in cell cultures of vitis amurensis. earlier, we transformed v. amurensis with the rolb gene of agrobacterium rhizogenes and showed increased level of resveratrol production in the rolb transgenic cell culture. we used control and the rolb-transgenic cell culture of v. amurensis as a model system in this study. ca was capable of increasing resveratrol producti ... | 2011 | 21938424 |
| Detection of protein interactions in plant using a gateway compatible bimolecular fluorescence complementation (BiFC) system. | We have developed a BiFC technique to test the interaction between two proteins in vivo. This is accomplished by splitting a yellow fluorescent protein (YFP) into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest. These constructs can then be co-transformed into Nicotiana benthamiana via Agrobacterium mediated transformation, allowing the transit expression of fusion proteins. The reconstitution of YFP signal only occurs when the inquest proteins interact (1-7 ... | 2011 | 21947026 |
| The Citrus leaf blotch virus movement protein acts as silencing suppressor. | To counteract plant antiviral defense based on RNA silencing, many viruses express proteins that inhibit this mechanism at different levels. The genome of Citrus leaf blotch virus (CLBV) encodes a 227-kDa protein involved in replication, a 40-kDa movement protein (MP), and a 41-kDa coat protein (CP). To determine if any of these proteins might have RNA silencing suppressor activities, we have used Agrobacterium-mediated transient assays in the green fluorescent protein (GFP)-expressing Nicotiana ... | 2011 | 21948005 |
| Methyl jasmonate elicitation enhances glycyrrhizin production in Glycyrrhiza inflata hairy roots cultures. | Hairy roots were induced by infecting stems and leaves of Glycyrrhiza inflata with Agrobacterium rhizogenes ATCC 15834. The optimization of growth and glycyrrhizin accumulation of G. inflata hairy roots was studied. Sucrose (6%, w/v) was optimal for growth and glycyrrhizin accumulation in G. inflata hairy roots. Effects of elicitors like chitosan, methyl jasmonate, and yeast extract on glycyrrhizin production were studied. Methyl jasmonate (100 microM) was most efficient in enhancing glycyrrhizi ... | 2011 | 21950168 |
| A guide to binary vectors and strategies for targeted genome modification in fungi using Agrobacterium tumefaciens-mediated transformation. | Agrobacterium tumefaciens-mediated transformation (ATMT) of fungi has become a common technique for the study of a wide variety of different fungal species over the past 12 years. The discovery that the host range of A. tumefaciens could be extended to include fungi provided an efficient transformation tool for species in which it was previously impossible to conduct molecular genetics experiments. ATMT experiments can be divided into three groups: i) Forward genetics (i.e., random mutagenesis), ... | 2011 | 21951772 |
| the agrobacterium-mediated transformation of common wheat (triticum aestivum l.) and triticale (x triticosecale wittmack): role of the binary vector system and selection cassettes. | the influence of two binary vector systems, pgreen and pcambia, on the agrobacterium-mediated transformation ability of wheat and triticale was studied. both vectors carried selection cassettes with bar or nptii driven by different promoters. two cultivars of wheat, kontesa and torka, and one cultivar of triticale, wanad, were tested. the transformation rates for the wheat cultivars ranged from 0.00 to 3.58% and from 0.00 to 6.79% for triticale. the best values for wheat were 3.58% for kontesa a ... | 2011 | 21952729 |
| Transient co-expression of post-transcriptional gene silencing suppressors for increased in planta expression of a recombinant anthrax receptor fusion protein. | Potential epidemics of infectious diseases and the constant threat of bioterrorism demand rapid, scalable, and cost-efficient manufacturing of therapeutic proteins. Molecular farming of tobacco plants provides an alternative for the recombinant production of therapeutics. We have developed a transient production platform that uses Agrobacterium infiltration of Nicotiana benthamiana plants to express a novel anthrax receptor decoy protein (immunoadhesin), CMG2-Fc. This chimeric fusion protein, de ... | 2011 | 21954339 |
| morphological and physiological characteristics of transgenic cherry tomato mutant with hbsag gene. | hbsag gene was previously introduced into cherry tomato (lycopersicum esculentum var. cerasiforme) by agrobacterium-mediated transformation. to investigate the side effect of hbsag gene in cherry tomato, we analyzed morphological and physiological characteristics of the transgenic mutant n244. the process was performed under field conditions. the results suggested that the mutant n244 exhibited morphological, cytological and physiological variation. first of all, compared with the wild plants nk ... | 2011 | 21954613 |
| gateway-compatible vectors for high-throughput gene functional analysis in switchgrass (panicum virgatum l.) and other monocot species. | switchgrass (panicum virgatum l.) is a c4 perennial grass and has been identified as a potential bioenergy crop for cellulosic ethanol because of its rapid growth rate, nutrient use efficiency and widespread distribution throughout north america. the improvement of bioenergy feedstocks is needed to make cellulosic ethanol economically feasible, and genetic engineering of switchgrass is a promising approach towards this goal. a crucial component of creating transgenic switchgrass is having the ca ... | 2011 | 21955653 |
| efficient embryogenic suspension culturing and rapid transformation of a range of elite genotypes of sweet potato (ipomoea batatas [l.] lam.). | efficient agrobacterium tumefaciens-mediated transformation was developed using embryogenic suspension cell cultures of elite sweet potato (ipomoea batatas [l.] lam.) cultivars, including ayamurasaki, sushu2, sushu9, sushu11, wanshu1, xushu18 and xushu22. embryogenic suspension cultures were established in lcp medium using embryogenic calli induced from apical or axillary buds on an induction medium containing 2 mg l(-1) 2,4-d. suspension cultures were co-cultivated with a. tumefaciens strain lb ... | 2011 | 21958713 |
| enhancing transgenic pea (pisum sativum l.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase). | one way of enhancing and broadening resistance of plants to different biotic and abiotic stresses is to combine transgenes expressing several genes into a single line. this can be done using different strategies such as crossing, single vector with multiple genes, co-transformation, sequential transformation and ires elements. in the present study conventional crossing method was used. parental transgenic lines transformed via agrobacterium tumefasciens-mediated gene transformation with pgreenii ... | 2011 | 21971070 |
| expression of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus in soybean seed yields an immunogenic antigenic protein. | porcine reproductive and respiratory syndrome (prrs), caused by the prrs virus (prrsv), is a serious disease of swine and contributes to severe worldwide economic losses in swine production. current vaccines against prrs rely on the use of an attenuated-live virus; however, these are unreliable. thus, alternative effective vaccines against prrs are needed. plant-based subunit vaccines offer viable, safe, and environmentally friendly alternatives to conventional vaccines. in this study, efforts h ... | 2011 | 21971995 |
| enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase. | arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that world health organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. this translates into an increased risk of arsenic-related illnesses for millions of people. recent studies demonstrate that increasing thiol-sinks in tran ... | 2011 | 21972493 |
| evolution of vacuolar proton pyrophosphatase domains and volutin granules: clues into the early evolutionary origin of the acidocalcisome. | volutin granules appear to be universally distributed and are morphologically and chemically identical to acidocalcisomes, which are electron-dense granular organelles rich in calcium and phosphate, whose functions include storage of phosphorus and various metal ions, metabolism of polyphosphate, maintenance of intracellular ph, osmoregulation and calcium homeostasis. prokaryotes are thought to differ from eukaryotes in that they lack membrane-bounded organelles. however, it has been demonstrate ... | 2011 | 21974828 |
| Evidence of Methylobacterium spp. and Hyphomicrobium sp. in azaspiracid toxin contaminated mussel tissues and assessment of the effect of azaspiracid on their growth. | A flagellar protein belonging to the genus Methylobacterium or Agrobacterium was previously observed by proteomics in azaspiracids (AZA) toxic mussels. Here, we report the isolation of two different Methylobacterium spp. (NTx1 and Tx1) from non-toxic and AZA toxic mussels, respectively, which when co-cultured with AZA exhibited significantly different growth responses - isolate Tx1 growth rate was enhanced, whereas growth of isolate NTx1 was adversely affected, compared to non-AZA supplemented c ... | 2011 | 21982824 |
| brucella abortus efp gene is required for an efficient internalization in hela cells. | numerous chromosomal virulence genes (chv) have been shown to play an important role in the ability of agrobacterium tumefaciens to transform plants. the a. tumefaciens chvh gene encodes a protein similar in sequence to the escherichia coli elongation factor p (ef-p). in a. tumefaciens this factor is required for tumor formation and for full expression of the vir genes, exerting its activity at a post-transcriptional level. cross-complementation assays suggest that the chvh gene and the efp gene ... | 2012 | 21983596 |
| Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue. | Small heat shock proteins are involved in stress tolerance. We previously isolated and characterized a rice cDNA clone, Oshsp26, encoding a chloroplast-localized small heat shock protein that is expressed following oxidative or heat stress. In this study, we transferred this gene to tall fescue plants by an Agrobacterium-mediated transformation system. The integration and expression of the transgene was confirmed by PCR, Southern, northern, and immunoblot analyzes. Compared to the control plants ... | 2011 | 21984008 |
| characterization of the mmsab-arad1 (gguabc) genes of agrobacterium tumefaciens. | the chve-gguabc operon plays a critical role in both virulence and sugar utilization through the activities of the periplasmic chve protein, which binds to a variety of sugars. the roles of the ggua, ggub, and gguc are not known. while ggua and ggub are homologous to bacterial abc transporters, earlier genetic analysis indicated that they were not necessary for utilization of sugars as the sole carbon source. to further examine this issue, in-frame deletions were constructed separately for each ... | 2011 | 21984786 |
| compositional equivalence of insect-protected glyphosate-tolerant soybean mon 87701 × mon 89788 to conventional soybean extends across different world regions and multiple growing seasons. | the soybean product mon 87701 × mon 89788 expresses both the cry1ac gene derived from bacillus thuringiensis and the cp4 epsps (5-enolpyruvylshikimate-3-phosphate synthase) gene derived from agrobacterium sp. strain cp4. each biotechnology-derived trait confers specific benefits of insect resistance and glyphosate tolerance, respectively. the purpose of this study was to compare the composition of seed and forage from this combined-trait product to those of conventional soybean grown in geog ... | 2011 | 21985102 |
| selection of reference genes for qpcr in hairy root cultures of peanut. | abstract: | 2011 | 21985172 |
| a pair of new bac and bibac vectors that facilitate bac/bibac library construction and intact large genomic dna insert exchange. | abstract: | 2011 | 21985432 |
| Plant expressed EtMIC2 is an effective immunogen in conferring protection against chicken coccidiosis. | Coccidiosis is an economically important disease affecting poultry industry and remains one of the major problems globally. Developing a cost effective sub-unit vaccine may help mitigate loss in the industry. Here, we report expressing one of the microneme proteins, EtMIC2 from Eimeria tenella in tobacco using Agrobacterium-mediated transient expression. The ability of plant expressed recombinant EtMIC2 in eliciting both humoral and cell-mediated immune responses were measured in the immunized b ... | 2011 | 21986219 |
| Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium. | Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The recombinant strain S. cerevisiae W303-1A/pAGROXI successfully colonized a minimal medium containing D: -xylose as a sole carbon source and was ca ... | 2011 | 21989637 |
| agrobacterium rhizogenes-mediated transformation of the parasitic plant phtheirospermum japonicum. | plants within the orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. | 2011 | 21991355 |
| regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9aa2 gene conferring insect resistance. | the recovery of high performing transgenic lines in clonal crops is limited by the occurrence of somaclonal variation during the tissue culture phase of transformation. this is usually circumvented by developing large populations of transgenic lines, each derived from the first shoot to regenerate from each transformation event. this study investigates a new strategy of assessing multiple shoots independently regenerated from different transformed cell colonies of potato (solanum tuberosum l.). | 2011 | 21995716 |
| Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection. | The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express ... | 2011 | 22000049 |
| increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic arabidopsis. | increasing the energy density of biomass by engineering the accumulation of triacylglycerols (tags) in vegetative tissues is synergistic with efforts to produce biofuels by conversion of lignocellulosic biomass. typically, tag accumulates in developing seeds, and little is known about the regulatory mechanisms and control factors preventing oil biosynthesis in vegetative tissues in most plants. here, we engineered arabidopsis thaliana to ectopically overproduce the transcription factor wrinkled1 ... | 2011 | 22003502 |
| an efficient agrobacterium-mediated transient transformation of arabidopsis. | agrobacterium tumefaciens-mediated transient transformation has been a useful procedure for characterization of proteins and their functions in plants, including analysis of protein-protein interactions. agrobacterium-mediated transient transformation of nicotiana benthamiana by leaf infiltration has been widely used due to its ease and high efficiency. however, in arabidopsis this procedure has been challenging. previous studies suggested that this difficulty was caused by plant immune response ... | 2011 | 22004025 |
| vanadium bromoperoxidase from delisea pulchra: enzyme-catalyzed formation of bromofuranone and attendant disruption of quorum sensing. | vanadium bromoperoxidase was isolated and cloned from the marine red alga delisea pulchra. this enzyme catalyzes the bromolactonization of 4-pentynoic acid forming 5e-bromo-methylidenetetrahydro-2-furanone, a compound which is shown herein to inhibit quorum sensing in the engineered reporter strain, agrobacterium tumefaciens ntl4. | 2011 | 22006105 |
| agrobacterium-mediated transformation of eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance. | eucalyptus globulus is one of the most economically important plantation hardwoods for paper making. however, its low transformation frequency has prevented genetic engineering of this species with useful genes. we found the hypocotyl section with a shoot apex has the highest regeneration ability among another hypocotyl sections, and have developed an efficient agrobacterium-mediated transformation method using these materials. we then introduced a salt tolerance gene, namely a bacterial choline ... | 2012 | 22009051 |
| agrobacterium counteracts host-induced degradation of its effector f-box protein. | the scf (skp1-cul1-f-box protein) ubiquitin ligase complex plays a pivotal role in various biological processes, including host-pathogen interactions. many pathogens exploit the host scf machinery to promote efficient infection by translocating pathogen-encoded f-box proteins into the host cell. how pathogens ensure sufficient amounts of the f-box effectors in the host cell despite the intrinsically unstable nature of f-box proteins, however, remains unclear. we found that the agrobacterium f-bo ... | 2011 | 22009152 |
| modified expression of alternative oxidase in transgenic tomato and petunia affects the level of tomato spotted wilt virus resistance. | abstract: | 2011 | 22014312 |
| Agrobacterium rhizogenes-dependent production of transformed roots from foliar explants of pepper (Capsicum annuum): a new and efficient tool for functional analysis of genes. | Pepper is known to be a recalcitrant species to genetic transformation via Agrobacterium tumefaciens. A. rhizogenes-mediated transformation offers an alternative and rapid possibility to study gene functions in roots. In our study, we developed a new and efficient system for A. rhizogenes transformation of the cultivated species Capsicum annuum. Hypocotyls and foliar organs (true leaves and cotyledons) of Yolo Wonder (YW) and Criollo de Morelos 334 (CM334) pepper cultivars were inoculated with t ... | 2011 | 22016085 |
| [construction of expression vector driven by tobacco root-specific promoter and transformation into rhodiola hairy root]. | to construct root-specific promoter of plant expression vector and transformate into rhodiola hairy root. | 2011 | 22017001 |
| expression and large-scale production of the biochemically active human tissue-plasminogen activator in hairy roots of oriental melon (cucumis melo). | human tissue-plasminogen activator (t-pa) is a thrombolytic protein that plays an active role in dissolving fibrin clots by fibrinolysis and in activating plasminogen to plasmin in blood vessels. t-pa and synthetic t-pa (st-pa) genes were expressed as enzymatically active form in hairy roots of oriental melon (cucumis melo l. cv. geumssaragi-euncheon) infected by agrobacterium rhizogenes. the insertion of the t-pa genes in genomic dna of transgenic hairy roots was verified by pcr. the presence a ... | 2011 | 22018738 |
| Improving UV resistance and virulence of Beauveria bassiana by genetic engineering with an exogenous tyrosinase gene. | Insect pathogenic fungi like Beauveria bassiana have been developed as environmentally friendly biocontrol agents against arthropod pests. However, restrictive environmental factors, including solar ultraviolet (UV) radiation frequently lead to inconsistent field performance. To improve resistance to UV damage, we used Agrobacterium-mediated transformation to engineer B. bassiana with an exogenous tyrosinase gene. The results showed that the mitotically stable transformants produced larger amoun ... | 2012 | 22024554 |
| Membrane and core periplasmic Agrobacterium tumefaciens virulence Type IV secretion system components localize to multiple sites around the bacterial perimeter during lateral attachment to plant cells. | Type IV secretion systems (T4SS) transfer DNA and/or proteins into recipient cells. Here we performed immunofluorescence deconvolution microscopy to localize the assembled T4SS by detection of its native components VirB1, VirB2, VirB4, VirB5, VirB7, VirB8, VirB9, VirB10, and VirB11 in the C58 nopaline strain of Agrobacterium tumefaciens, following induction of virulence (vir) gene expression. These different proteins represent T4SS components spanning the inner membrane, periplasm, or outer memb ... | 2011 | 22027007 |
| extracellular virb5 enhances t-dna transfer from agrobacterium to the host plant. | virb5 is a type 4 secretion system protein of agrobacterium located on the surface of the bacterial cell. this localization pattern suggests a function for virb5 which is beyond its known role in biogenesis and/or stabilization of the t-pilus and which may involve early interactions between agrobacterium and the host cell. here, we identify virb5 as the first agrobacterium virulence protein that can enhance infectivity extracellularly. specifically, we show that elevating the amounts of the extr ... | 2011 | 22028781 |
| a high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots. | barley stripe mosaic virus (bsmv) is a single-stranded rna virus with three genome components designated alpha, beta, and gamma. bsmv vectors have previously been shown to be efficient virus induced gene silencing (vigs) vehicles in barley and wheat and have provided important information about host genes functioning during pathogenesis as well as various aspects of genes functioning in development. to permit more effective use of bsmv vigs for functional genomics experiments, we have developed ... | 2011 | 22031834 |
| virus-derived ssdna vectors for the expression of foreign proteins in plants. | plant viruses with ssrna genomes provide a unique opportunity for generating expression vehicles for biopharming in plants, as constructs containing only the replication origin, with the replication-associated protein (rep) gene provided in cis or in trans, can be replicationally amplified in vivo by several orders of magnitude, with significant accompanying increases in transcription and expression of gene(s) of interest. appropriate replicating vectors or replicons may be derived from several ... | 2011 | 22038412 |
| generation of transgenic rice lines with reduced contents of multiple potential allergens using a null mutant in combination with an rna silencing method. | rice seed proteins are known to be a causative antigen in some patients with food allergy, especially cereal allergy, with clinical symptoms such as eczema and dermatitis. the α-amylase/trypsin inhibitors (14-16 kda), α-globulin (26 kda) and β-glyoxalase i (33 kda) are regarded as major potential allergens of rice (oryza sativa l.) seed based on specific recognition by serum ige from allergy patients. in order to suppress the production of these major allergens in rice grains, a mutant in the 'k ... | 2011 | 22039121 |
| Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study. | Approaches to simplify and streamline the construction of full-length infectious cDNA clones (FL-cDNAs) are needed. Among desirable improvements are the ability to use total nucleic acids (TNA) extracts from infected hosts (to bypass viral purification limitations) for the direct one-step amplification of large FL-cDNAs, the possibility to inoculate plants with uncloned FL-cDNAs and the simplified cloning of these large molecules. | 2011 | 22040379 |
| temperature effects on agrobacterium phytochrome agp1. | phytochromes are widely distributed biliprotein photoreceptors with a conserved n-terminal chromophore-binding domain. most phytochromes bear a light-regulated c-terminal his kinase or his kinase-like region. we investigated the effects of light and temperature on the his kinase activity of the phytochrome agp1 from agrobacterium tumefaciens. as in earlier studies, the phosphorylation activity of the holoprotein after far-red irradiation (where the red-light absorbing pr form dominates) was stro ... | 2011 | 22043299 |
| [high efficient gene knockout in verticillium dahliae]. | we developed an efficient method of gene knockout in verticillium dahliae, an important soil-borne fungal pathogen that causes cotton vascular wilt diseases. | 2011 | 22043791 |
| natural plant genetic engineer agrobacterium rhizogenes: role of t-dna in plant secondary metabolism. | agrobacterium rhizogenes is a natural plant genetic engineer. it is a gram-negative soil bacterium that induces hairy root formation. success has been obtained in exploring the molecular mechanisms of transferred dna (t-dna) transfer, interaction with host plant proteins, plant defense signaling and integration to plant genome for successful plant genetic transformation. t-dna and corresponding expression of rol genes alter morphology and plant host secondary metabolism. dur ... | 2011 | 22048847 |
| dissimilatory ammonia production vs. denitrification in vitro and in inoculated agricultural soil samples. | a dissimilatory ammonia-producing isolate identified as enterobacter amnigenus and a denitrifier identified as agrobacterium radiobacter isolated from the same soil were studied. the products of nitrate reduction in a minimal medium, enriched with glucose and containing nitrate n as the sole nitrogen source, were quantified when each of these isolates was cultured anaerobically, alone or mixed together in the presence or absence of c(2)h(2). when they were cultured together, ammonia was the prin ... | 1990 | 22049939 |
| genetic transformation of colletotrichum truncatum associated with anthracnose disease of chili by random insertional mutagenesis. | an agrobacterium tumefaciens -mediated transformation (atmt) system was successfully developed for colletotrichum truncatum, the causal agent of chili anthracnose. a. tumefaciens carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (gfp) gene was used to transform the conidiospores of two c. truncatum pathotypes f8-3b and brip26974. optimum transformation efficiency was obtained when equal volumes of a. tumefaciens strain agl1 carrying either pjf1 or ppk2 binary ve ... | 2011 | 22052577 |
| surface contact stimulates the just-in-time deployment of bacterial adhesins. | the attachment of bacteria to surfaces provides advantages such as increasing nutrient access and resistance to environmental stress. attachment begins with a reversible phase, often mediated by surface structures such as flagella and pili, followed by a transition to irreversible attachment, typically mediated by polysaccharides. here we show that the interplay between pili and flagellum rotation stimulates the rapid transition between reversible and polysaccharide-mediated irreversible attachm ... | 2012 | 22053824 |
| construction of two vectors for gene expression in trichoderma reesei. | we report the construction of two filamentous fungi trichoderma reesei expression vectors, pwef31 and pwef32. both vectors possess the hygromycin phosphotransferase b gene expression cassette and the strong promoter and terminator of the cellobiohydrolase 1 gene (cbh1) from t. reesei. the two newly constructed vectors can be efficiently transformed into t. reesei with agrobacterium-mediated transformation. the difference between pwef31 and pwef32 is that pwef32 has two longer homologous arms. as ... | 2012 | 22056690 |
| a photolyase-like protein from agrobacterium tumefaciens with an iron-sulfur cluster. | photolyases and cryptochromes are evolutionarily related flavoproteins with distinct functions. while photolyases can repair uv-induced dna lesions in a light-dependent manner, cryptochromes regulate growth, development and the circadian clock in plants and animals. here we report about two photolyase-related proteins, named phra and phrb, found in the phytopathogen agrobacterium tumefaciens. phra belongs to the class iii cyclobutane pyrimidine dimer (cpd) photolyases, the sister class of plant ... | 2011 | 22066008 |
| [isolation and characterization of promoter of ads from artemisia annua]. | to try to find the ways to enhance the expression of ads gene encoding amorpha-4,11-diene synthase, a key enzyme in artemisinin biosynthesis pathway catalyzing the formation of amorpha-4,11-diene from farnesyl diphosphate, and accelerate the artemisinin synthesis, the promoter of ads was isolated and characterized. | 2011 | 22066438 |
| Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China. | A total of 159 endophytic bacteria were isolated from surface-sterilized root nodules of wild perennial Glycyrrhiza legumes growing on 40 sites in central and northwestern China. Amplified fragment length polymorphism (AFLP) genomic fingerprinting and sequencing of partial 16S rRNA genes revealed that the collection mainly consisted of Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium and Paenibacillus species. Based on symbiotic properties with the legume hosts Glycyrrhiza uralensis and Gl ... | 2011 | 22066910 |
| Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca. | ABSTRACT: BACKGROUND: Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites is difficult since their survival depends completely on successful haustorium penetration of a host and the formation of vascular connections. The recent completion of a massive transcriptome sequencing project (the Parasitic Plant Genome Project) will fuel the use o ... | 2011 | 22067615 |
| solanum lycopersicum cytokinin response factor (slcrf) genes: characterization of crf domain-containing erf genes in tomato. | cytokinin is an influential hormone in growth and developmental processes across many plant species. while several cytokinin-regulated genes have been well characterized in arabidopsis, few have been identified in tomato, solanum lycopersicum. here a tomato family of 11 highly related cytokinin response factor genes designated as slcrf1-slcrf11 (solanum lycopersicum cytokinin response factor) are identified and characterized. slcrfs are ap2/erf transcription factors and generally orthologous to ... | 2011 | 22068146 |
| telomere-mediated truncation of barley chromosomes. | engineered minichromosomes offer an enormous opportunity to plant biotechnology as they have the potential to simultaneously transfer and stably express multiple genes. following a top-down approach, we truncated endogenous chromosomes in barley (hordeum vulgare) by agrobacterium-mediated transfer of t-dna constructs containing telomere sequences. blocks of arabidopsis-like telomeric repeats were inserted into a binary vector suitable for stable transformation. after transfer of these constructs ... | 2011 | 22080935 |
| zinc finger nuclease and homing endonuclease-mediated assembly of multigene plant transformation vectors. | binary vectors are an indispensable component of modern agrobacterium tumefaciens-mediated plant genetic transformation systems. a remarkable variety of binary plasmids have been developed to support the cloning and transfer of foreign genes into plant cells. the majority of these systems, however, are limited to the cloning and transfer of just a single gene of interest. thus, plant biologists and biotechnologists face a major obstacle when planning the introduction of multigene traits into tra ... | 2011 | 22082504 |
| in vitro azadirachtin production by hairy root cultivation of azadirachta indica in nutrient mist bioreactor. | azadirachtin, a well-known biopesticide is a secondary metabolite conventionally extracted from the seeds of azadirachta indica. the present study involved in vitro azadirachtin production by developing hairy roots of a. indica via agrobacterium rhizogenes-mediated transformation of a. indica explants. liquid culture of hairy roots was established in shake flask to study the kinetics of growth and azadirachtin production. a biomass production of 13.3 g/l dry weight (specific growth rate of 0.7 d ... | 2011 | 22083394 |
| functional characterization of sugarcane myb transcription factor gene promoter (pscmybas1) in response to abiotic stresses and hormones. | the sugarcane (saccharum officinarum) stress-related myb transcription factor gene, scmybas1, demonstrated induced response to water deficit and salt stress in our previous study. to elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (pscmybas1, 1,033 bp) flanking the 5' scmybas1 coding region from the sugarcane genome. a series of pscmybas1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -8 ... | 2011 | 22083650 |
| Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation: lessons from transformation assays and genome-wide expression profiling. | Agrobacterium tumefaciens-mediated genetic transformation has been routinely used in rice for more than a decade. However, the transformation efficiency of the indica rice variety is still unsatisfactory and much lower than that of japonica cultivars. Further improvement on the transformation efficiency lies in the genetic manipulation of the plant itself, which requires a better understanding of the underlying process accounting for the susceptibility of plant cells to Agrobacterium infection a ... | 2012 | 22089972 |
| rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing. | we aimed to engineer transgenic plants for the purpose of early detection of plant pathogen infection, which was accomplished by employing synthetic pathogen inducible promoters fused to reporter genes for altered phenotypes in response to the pathogen infection. toward this end, a number of synthetic promoters consisting of inducible regulatory elements fused to a red fluorescent protein (rfp) reporter were constructed for use in phytosensing. | 2011 | 22093754 |
| [discovery and research progress of endophytic bacteria in the root nodules of legumes--a review]. | recently, some research reports showed varied endophytic bacteria in the root nodules of some legumes, which attracts great interest in research field of rhizobia. here, we reviewed the discovery, identification of some endophytic bacteria (agrobacterium, non-symbiotic rhizobia, and other bacteria) in root nodules and their influence on symbiosis or plant growth, to understand the microecosystem of root nodule and to extend the field of rhizobia research. | 2011 | 22097764 |
| [construction of a bivalent plant expression vector carrying vvsuc11 and vvsuc12 genes and its genetic transformation in sugar beet]. | we have recombined genes vvsuc11, vvsuc12 from vitis vinifera l., and root-specific promoters of sweet potato storage protein gene from ipomoea batatas l. lam., named as sp1 and sp2. we have constructed a vector pcambia2301-sp1- vvsuc11-sp2-vvsuc12 using pcambia2301 as an original vector. vvsuc11 and vvsuc12 were under the control of root-specific promoters of sweet potato storage protein gene. we transformed the vector into kws-9103 breeding line of beta vulgaris l. with agrobacterium-mediated ... | 2011 | 22097805 |
| detection of acylated homoserine lactones produced by vibrio spp. and related species isolated from water and aquatic organisms. | aims: to assess the diversity in production of acylated homoserine lactones (ahls) among vibrio spp and related species. methods and results: a total of 106 isolates, with representatives of 28 vibrio spp and related species, were investigated for the production of ahls. for this, a rapid method for the screening of ahls was developed based on the use of bacterial biosensors using a double-layer microplate assay. at least one bacterial biosensor was activated in 20 species, agrobacterium tumef ... | 2011 | 22098208 |
| Inhibition and dispersal of Agrobacterium tumefaciens biofilms by a small diffusible Pseudomonas aeruginosa exoproduct(s). | Environmental biofilms often contain mixed populations of different species. In these dense communities, competition between biofilm residents for limited nutrients such as iron can be fierce, leading to the evolution of competitive factors that affect the ability of competitors to grow or form biofilms. We have discovered a compound(s) present in the conditioned culture fluids of Pseudomonas aeruginosa that disperses and inhibits the formation of biofilms produced by the facultative plant patho ... | 2011 | 22105093 |
| identification of pathogenesis associated genes by t-dna-mediated insertional mutagenesis in botrytis cinerea: a type 2a phosphoprotein phosphatase and a spt3 transcription factor have significant impact on virulence. | agrobacterium tumefaciens - mediated transformation (atmt) was used to generate an insertional mutant library of the grey mould fungus botrytis cinerea. from a total of 2,367 transformants, 68 mutants showing significant reduction in virulence on tomato and bean plants were analyzed in detail. as reported for other fungal atmt libraries, integrations were mostly single copy, occurred preferentially in non-coding (regulatory) regions, and were frequently accompanied by small deletions of the targ ... | 2011 | 22112214 |
| Improvement of cellulase activity in Trichoderma reesei by heterologous expression of a beta-glucosidase gene from Penicillium decumbens. | Trichoderma reesei is a well-known cellulase producer and widely applied in enzyme industry. To increase its ability to efficiently decompose cellulose, the beta-glucosidase activity of its enzyme cocktail needs to be enhanced. In this study, a beta-glucosidase I coding sequence from Penicillium decumbens was ligated with the cellobiohydrolase I (cbh1) promoter of T. reesei and introduced into the genome of T. reesei strain Rut-C30 by Agrobacterium-mediated transformation. In comparison to that ... | 2011 | 22112562 |
| Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. | Halohydrin dehalogenases are attractive biocatalysts in producing a series of important chiral building blocks. Recombinant expression of halohydrin dehalogenase from Arthrobacter sp. AD2 (HheA) in Escherichia coli using T7 promoter-based pGEF(+) system revealed much lower expression level than that of the well-studied halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC). In this study, we changed the codon usage in the 5'-end of hheA gene to improve the expression yield of HheA. Ou ... | 2011 | 22112566 |
| Phosphate-bound structure of an organophosphate-degrading enzyme from Agrobacterium radiobacter. | OpdA is a binuclear metalloenzyme that can hydrolyze organophosphate pesticides and nerve agents. In this study the crystal structure of the complex between OpdA and phosphate has been determined to 2.20Å resolution. The structure shows the phosphate bound in a tripodal mode to the metal ions whereby two of the oxygen atoms of PO(4) are terminally bound to each metal ion and a third oxygen bridges the two metal ions, thus displacing the µOH in the active site. In silico modelling demonstrates th ... | 2012 | 22112835 |
| a cooperative virulence plasmid imposes a high fitness cost under conditions that induce pathogenesis. | harbouring a plasmid often imposes a fitness cost on the bacterial host. motivated by implications for public health, the majority of studies on plasmid cost are focused on elements that impart antibiotic resistance. plasmids, however, can provide a wide range of ecologically important phenotypes to their bacterial hosts-such as virulence, specialized catabolism and metal resistance. the agrobacterium tumefaciens tumour-inducing (ti) plasmid confers both the ability to infect dicotyledonous plan ... | 2011 | 22113028 |
| Enhancement of rutin in Fagopyrum esculentum hairy root cultures by the Arabidopsis transcription factor AtMYB12. | Common buckwheat (Fagopyrum esculentum Moench) is rich in phenolic compounds and may be useful for the treatment of metabolic syndrome in humans. To improve the production of rutin in buckwheat, we overexpressed the flavonol-specific transcription factor, AtMYB12 using Agrobacterium rhizogenes into hairy root culture systems. This induced the expression of flavonoid biosynthetic genes encoding phenylalanine ammonia lyase, cinnamate 4-hydroxylase, 4-coumarate:CoA ligase, chalcone synthase, chalco ... | 2011 | 22113884 |
| supramolecular complexes of the agrobacterium tumefaciens virulence protein vire2. | virulence protein vire2 from agrobacterium tumefaciens is involved in plant infection by transferring a fragment of agrobacterial ti plasmid sst-dna in complex with vire2-vird2 proteins into the plant cell nucleus. the vire2 protein interactions with ssdna and formation of vire2 protein complexes in vitro and in silico have been studied. using dynamic light scattering we found that purified recombinant protein vire2 exists in buffer solution in the form of complexes of 2-4 protein molecules of 1 ... | 2011 | 22117554 |
| [6b genes: the important effective factors relative to tumor formation in plants]. | In recent years, the functional mechanisms of the oncogenens from Agrobacterium in plants were received more and more attentions. 6b genes, derived from the T-DNA fragment, are vital carcinogenesis factors of plants and belong to rolB genes family. In plants, 6b genes can affect phytohormone levels and carbohydrate contents, and can also cause accumulation of secondary metabolites, as well as change the relative genes expression. The specific mechanisms behind these impacts remain to be research ... | 2011 | 22120076 |
| recent advances in curdlan biosynthesis, biotechnological production, and applications. | curdlan is a water-insoluble β-(1,3)-glucan produced by agrobacterium species under nitrogen-limited condition. its heat-induced gelling properties render curdlan to be very useful in the food industry initially. recent advances in the understanding of the role curdlan plays in both innate and adaptive immunity lead to its growing applications in biomedicine. our review focuses on the recent advances on curdlan biosynthesis and the improvements of curdlan fermentation production both from our la ... | 2011 | 22124723 |
| Production of mouse granulocyte-macrophage colony-stimulating factor by gateway technology and transgenic rice cell culture. | To establish a production platform for recombinant proteins in rice suspension cells, we first constructed a Gateway-compatible binary T-DNA destination vector. It provided a reliable and effective method for the rapid directional cloning of target genes into plant cells through Agrobacterium-mediated transformation. We used the approach to produce mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) in a rice suspension cell system. The promoter for the aAmy3 amylase gene, which is ... | 2011 | 22125231 |
| Antibacterial Activities of Ginkgo biloba L. Leaf Extracts. | The antibacterial activity of methanol, ethanol, chloroform, and hexane extracts of the leaves of Himalayan gymnospermous plant Ginkgo biloba L. was assessed against five animal and plant pathogenic strains (Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, Erwinia chrysanthemi, and Xanthomonas phaseoli) employing disc-diffusion and broth-dilution assays. The methanol extract showed the highest activity (zone of inhibition of 15-21?mm) followed by ethanol (14-19?mm), chloroform (15 ... | 2011 | 22125471 |
| [detection of the quorum sensing signals in methanogenic archaea]. | methanosaeta harundinacea 6ac displayed a cell-density dependent cell morphology transition between short rods (3 microm - 5 microm) and filaments ( >200 microm), implying a quorum sensing system in this archaeon. | 2011 | 22126075 |
| Biodegradation of crystal violet by Agrobacterium radiobacter. | Agrobacterium radiobacter MTCC 8161 completely decolorized the Crystal Violet with 8 hr (10 mg/L) at static anoxic conditions. The decreased decolorization capability by A. radiobacter was observed, when the Crystal Violet concentration was increased from 10 to 100 mg/L. Semi-synthetic medium containing 1% yeast extract and 0.1% NH4C1 has shown 100% decolorization of Crystal Violet within 5 hr. A complete degradation of Crystal Violet by A. radiobacter was observed up to 7 cycles of repeated add ... | 2011 | 22128547 |
| Use of the Cre-loxP recombination system as an estimate for Agrobacterium-mediated co-transformation of tobacco leaves. | Agrobacterium tumefaciens-mediated transformation of tobacco leaves (Nicotiana tabacum) is used to study gene expression in a heterologous genetic background. Here, the Cre-loxP recombination system was used to detect T-DNA transfer by two A. tumefaciens cells harboring different binary vectors. Cre, under the control of the CaMV 35S promoter, was cloned into one vector, and a loxP cassette into another vector. A mixture of A. tumefaciens, in which each ... | 2011 | 22130741 |
| Phylogenomics of Reichenowia parasitica, an Alphaproteobacterial Endosymbiont of the Freshwater Leech Placobdella parasitica. | Although several commensal alphaproteobacteria form close relationships with plant hosts where they aid in (e.g.,) nitrogen fixation and nodulation, only a few inhabit animal hosts. Among these, Reichenowia picta, R. ornata and R. parasitica, are currently the only known mutualistic, alphaproteobacterial endosymbionts to inhabit leeches. These bacteria are harbored in the epithelial cells of the mycetomal structures of their freshwater leech hosts, Placobdella spp., and these structures have no ... | 2011 | 22132238 |
| glucose and plant exudate enhanced enumeration of bacteria capable of degrading polycyclic aromatic hydrocarbons. | enumerating environmental microbial isolates capable of polycyclic aromatic hydrocarbon (pah) degradation can provide insight into the microbe-plant interactions that facilitate pah removal. we examined a known pah degrader ( pseudomonas putida g7), a nondegrader ( agrobacterium tumefaciens lba4404), and several microorganisms isolated from the environment by using a pah cocktail in an enumeration medium with or without 0.025% (m/v) glucose and (or) root exudates. compared with the standard m ... | 2011 | 22136124 |
| Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater. | Olive oil mill wastewaters (OMWs) are characterised by low pH and a high content of mono- and polyaromatic compounds that exert microbial and phytotoxic activity. The laccase cDNA of the poxA1b gene from Pleurotus ostreatus, carrying a signal peptide sequence for enzyme secretion and driven by the CaMV 35S promoter, was cloned into a plant expression vector. Nuclear genetic transformation was carried out by co-cultivation of Agrobacterium tumefaciens with tobacco cv Samsun NN leaves and cells of ... | 2011 | 22142729 |
| asymmetric leaves2 gene, a member of lob/as2 family of arabidopsis thaliana, causes an abaxializing leaves in transgenic cockscomb. | the leaf primordium derives from the peripheral zone of shoot apical meristem. during the formation of leaf primordia, they need to establish the proximodistal, mediolateral, and ab/adaxial axes. among these axes, the ab/adaxial axis might be the most important. asymmetric leaves2 (as2) gene is a member of as2/lateral organ boundary (lob) family of arabidopsis thaliana. in this work, we transformed 35s:as2 transgene constructs to cockscomb (celosia cristata) via agrobacterium ... | 2011 | 22143880 |
| a novel gateway®-compatible binary vector allows direct selection of recombinant clones in agrobacterium tumefaciens. | abstract: | 2011 | 22145613 |
| the conjugation protein tcpc from clostridium perfringens is structurally related to the type iv secretion system protein virb8 from gram-negative bacteria. | bacterial conjugation is important for the acquisition of virulence and antibiotic resistance genes. we investigated the mechanism of conjugation in gram-positive pathogens using a model plasmid pcw3 from clostridium perfringens. pcw3 encodes tetracycline resistance and contains the tcp locus, which is essential for conjugation. we showed that the unique tcpc protein (359 amino acids, 41 kda) was required for efficient conjugative transfer, localized to the cell membrane independently of other c ... | 2011 | 22150951 |
| high level expression of acidothermus cellulolyticus beta-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid. | abstract: background: large-scale production of effective cellulose hydrolytic enzymes is the key to bioconversion of agricultural residues to ethanol. the goal of this study was to develop rice plant as a bioreactor for large-scale production of cellulose hydrolytic enzymes via genetic transformation and to improve rice straw simultaneously as an efficient biomass feedstock for conversion of cellulose to glucose. results: in this study, the cellulose hydrolytic enzyme beta-1, 4-endoglucanase ( ... | 2011 | 22152050 |
| international committee on systematics of prokaryotes subcommittee on the taxonomy of agrobacterium and rhizobium: minutes of the meeting, 7 september 2010, geneva, switzerland. | 2011 | 22156799 | |
| modulation of ethanol stress tolerance by aldehyde dehydrogenase in the mycorrhizal fungus tricholoma vaccinum. | we report the first mycorrhizal fungal aldehyde dehydrogenase gene, ald1, which was isolated from the basidiomycete tricholoma vaccinum. the gene, encoding a protein ald1 of 502 amino acids, is up-regulated in ectomycorrhiza. phylogenetic analyses using 53 specific fungal aldehyde dehydrogenases from all major phyla in the kingdom of fungi including ald1 and two partial sequences of t. vaccinum were performed to get an insight in the evolution of the aldehyde dehydrogenase family. by using compe ... | 2011 | 22159964 |
| stress-inducible expression of barley hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress. | coping with different kinds of biotic and abiotic stresses is the foundation of sustainable agriculture. although conventional breeding and marker-assisted selection are being employed in mulberry (morus indica l.) to develop better varieties, nonetheless the longer time periods required for these approaches necessitates the use of precise biotechnological approaches for sustainable agriculture. in an attempt to improve stress tolerance of mulberry, an important plant of the sericulture industry ... | 2011 | 22160463 |
| Molecular cloning and functional characterization of Catharanthus roseus hydroxymethylbutenyl 4-diphosphate synthase gene promoter from the methyl erythritol phosphate pathway. | The Madagascar periwinkle produces monoterpenoid indole alkaloids (MIA) of high interest due to their therapeutical values. The terpenoid moiety of MIA is derived from the methyl erythritol phosphate (MEP) and seco-iridoid pathways. These pathways are regarded as the limiting branch for MIA biosynthesis in C. roseus cell and tissue cultures. In previous studies, we demonstrated a coordinated regulation at the transcriptional and spatial levels of genes from both pathways. We report here on the i ... | 2011 | 22160472 |
| agrobacterium tumefaciens-mediated transformation of aspergillus aculeatus for insertional mutagenesis. | abstract: agrobacterium tumefaciens-mediated transformation (amt) was applied to aspergillus aculeatus. transformants carrying the t-dna from a binary vector pbig2rhph2 were sufficiently mitotically stable to allow functional genomic analyses. the amt technique was optimized by altering the concentration of acetosyringone, the ratio and concentration of a. tumefaciens and a. aculeatus cells, the duration of co-cultivation, and the status of a. aculeatus cells when using conidia, protoplasts, o ... | 2011 | 22166586 |
| [Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots]. | Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon alpha2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants. | 2011 | 22168044 |