Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography. | hydrophobic interaction chromatography (hic) is an important technique for protein purification, which exploits the separation of proteins based on hydrophobic interactions between the stationary phase ligands and hydrophobic regions on the protein surface. one way of enhancing the purification efficiency by hic is the addition of short sequences of peptide tags to the target protein by genetic engineering, which could reduce the need for extra and expensive chromatographic steps. in the present ... | 2007 | 17448484 |
| evaluation of the inhibitory effect of amphotericin b on the apical growth of f. solani using the biocell-tracer system. | the biocell-tracer (bct) system is an automatic microscopic method used for measuring the growth rate of a single fungal hyphae, which has not yet been applied to study fusarium spp. considering the large resistance of fusarium species to the available chemotherapy and that hyphae is the morphological fungal form most often seen in vivo, in this work, amphotericin b mic and mfc values for a fusarium solani strain were obtained by the conventional assay method testing conidia and also by the bct ... | 2007 | 17472613 |
| quality control and reference guidelines for clsi broth microdilution method (m38-a document) for susceptibility testing of anidulafungin against molds. | the clsi (formerly nccls) m38-a document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. a multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (mics and minimum effective concentrations [mecs]) for evaluating anidulafungin against molds. anidulafungin mics (>or=50% inhibition) and mecs (morphological hyphal changes) were determined f ... | 2007 | 17475760 |
| biotransformation of heterocyclic dinitriles by rhodococcus erythropolis and fungal nitrilases. | 2,6-pyridinedicarbonitrile (1a) and 2,4-pyridinedicarbonitrile (2a) were hydrated by rhodococcus erythropolis a4 to 6-cyanopyridine-2-carboxamide (1b; 83% yield) and 2-cyanopyridine-4-carboxamide (2b; 97% yield), respectively, after 10 min. after 118 h, the intermediates 1b or 2b were transformed into 2,6-pyridinedicarboxamide (1c; 35% yield) and 2,6-pyridinedicarboxylic acid (1d; 60% yield) or 2-cyanopyridine-4-carboxylic acid (2c; 64% yield), respectively. the nitrilase from fusarium solani af ... | 2007 | 17479225 |
| chemical modification results in hyperactivation and thermostabilization of fusarium solani glucoamylase. | chemical modification of carboxyl groups of glucoamylase from a mesophilic fungus, fusarium solani, was carried out using ethylenediamine as nucleophile in the presence of water-soluble 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. modification brought about a dramatic enhancement of catalytic activity and thermal stability of glucoamylase. temperature and ph optima of ethylenediamine-coupled glucoamylase (ecg) increased as compared with those of native enzyme. the specificity constant (k(cat)/k ... | 2007 | 17496965 |
| metal-based antibacterial and antifungal agents: synthesis, characterization, and in vitro biological evaluation of co(ii), cu(ii), ni(ii), and zn(ii) complexes with amino acid-derived compounds. | a series of antibacterial and antifungal amino acid-derived compounds and their cobalt(ii), copper(ii), nickel(ii), and zinc(ii) metal complexes have been synthesized and characterized by their elemental analyses, molar conductances, magnetic moments, and ir, and electronic spectral measurements. ligands (l(1))-(l(5)) were derived by condensation of beta-diketones with glycine, phenylalanine, valine, and histidine and act as bidentate towards metal ions (cobalt, copper, nickel, and zinc) via the ... | 2006 | 17497020 |
| production of fusarium solani f. sp. pisi cutinase in fusarium venenatum a3/5. | fusarium venenatum a3/5 was transformed using the aspergillus niger expression plasmid, pigf, in which the coding sequence for the f. solani f. sp. pisi cutinase gene had been inserted in frame, with a kex2 cleavage site, with the truncated a. niger glucoamylase gene under control of the a. niger glucoamylase promoter. the transformant produced up to 21 u cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. glucoamylase (165 u l(-1) or 8 mg l(-1)) was also ... | 2007 | 17505784 |
| expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis. | levels of matrix metalloproteinases (mmps) can be modulated during corneal infection, but little is known about mmp profiles during fungal keratitis. the purpose of this study was to determine the effect of corneal trauma and immunosuppressive treatment on the expression kinetics of mmp-2 and mmp-9 during experimental keratomycosis. | 2007 | 17525657 |
| fungal neuroinfections: rare disease but unacceptably high mortality. | within last 25 years we have observed 20 cases of fungal meningitis and/or cerebral abscesses. commonest etiologic agens was candida spp. (c. albicans 9 of 20). molds were responsible for 4 cases of brain abscess. mortality was 50% what seems to be very high. extremely high mortality is caused by delayed onset of therapy, severe underlying disease and multiresistant fungal organisms such as mucorales, fusarium solani and aureobasidium. | 2007 | 17558372 |
| expression of a ribosome-inactivating protein gene in bitter melon is induced by sphaerotheca fuliginea and abiotic stimuli. | the gene encoding a single-chain, ribosome-inactivating protein (scrip) was cloned from bitter melon (momordica charantia l.) leaves infected with the fungus, sphaerotheca fuliginea, by rt-pcr. the orf was 861 bp. the ribosome-inactivating protein was expressed in e. coli and, when purified, it inhibited the growth of fusarium solani [corrected] northern blot analysis revealed that rip transcripts rapidly accumulated in leaves 1-day post inoculation with sphaerotheca fuliginea and reached a peak ... | 2007 | 17579811 |
| efficacy of topical caspofungin in experimental fusarium keratitis. | to evaluate the efficacy of caspofungin in an experimental rabbit model of fusarium keratitis and to compare it with amphotericin b. | 2007 | 17592325 |
| in vitro antifungal activity of rhazya stricta. | rhazya stricta is a small glabrous shrub, widely distributed throughout western asia from yemen to arabia, to the north west province of india and abundantly found in various regions of pakistan. larvicidal and antifungal studies of polar and non polar aerial parts extracts of rhazya stricta were performed using brine shrimps larvae for larvicidal study and for antifungal study microorganisms, trichophyton longifusis, aspergillus flavus, candida albicans, microsporum canis and fusarium solani we ... | 2007 | 17604249 |
| [experimental study of fast diagnosis of mycotic keratitis using molecular biology technical]. | to establish a method for fast diagnosis of mycotic keratitis using multi-pcr system. | 2007 | 17605910 |
| comparison of pathogen-induced expression and efficacy of two amphibian antimicrobial peptides, msra2 and temporin a, for engineering wide-spectrum disease resistance in tobacco. | the rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. in this study, two antimicrobial peptides from the skin secretions of frogs, msra2 (n-methionine-dermaseptin b1) and temporin a, were evaluated for engineering pathogen-induced disease resistance in plants. both peptides inhibited plant-specific pathogens in vitro at micromolar concentrations that were not toxic to plant protoplasts. the plant-optimiz ... | 2007 | 17645440 |
| interaction of fusarium solani lectin with monosaccharides and oligosaccharides: a fluorometric study. | the intrinsic fluorescence intensity of fusarium solani lectin was quenched upon binding to mono- and oligosaccharides without any change in the emission maximum and it was used to determine association constants for several sugars and glycans. the lectin interacted very poorly with monosaccharides but well with disaccharides (t-antigen and lacnac) with a distinction between beta1-->4 and beta1-->3 linkages. among the monosaccharides, the interaction was observed only with gal/galnac derivatives ... | 2007 | 17645671 |
| toxigenic fungi and mycotoxins in mature corn silage. | to investigate the exposure of livestock and farm workers to mycotoxins during the last months of silage use, the mycoflora and the mycotoxins in a mature silage (11-months-old) were studied. a multimycotoxin method was developed to evaluate the toxigenic in vitro ability of fungal strains. the screening of potentially toxigenic fungi isolated from the mature silage showed that six fusaria (fusarium culmorum, fusarium equiseti, fusarium graminearum, fusarium oxysporum, fusarium solani and fusari ... | 2007 | 17655998 |
| discovery of fusarium solani as a naturally occurring pathogen of sugarbeet root maggot (diptera: ulidiidae) pupae: prevalence and baseline susceptibility. | the fungus fusarium solani (mart.) sacc. was discovered as a native entomopathogen of the sugarbeet root maggot, tetanops myopaeformis (röder), in the red river valley of north dakota during the 2004 sugarbeet production season. this is the first report of a native pathogen affecting the pupal stage of t. myopaeformis. forty-four percent of larvae collected from a field site near st. thomas (pembina co.) in northeastern north dakota during may and june of 2004 were infected with the entomopathog ... | 2008 | 17662996 |
| attachment to and penetration of conventional and silicone hydrogel contact lenses by fusarium solani and ulocladium sp. in vitro. | to analyze the relative capacities of fusarium solani and ulocladium sp. to attach to and penetrate silicone hydrogel soft contact lenses. | 2007 | 17667618 |
| [the effect of infection by fusarium solani f. sp. batatas on endogenous hormone levels in sweet potato seedling]. | the endogenous hormone aba concentrations increased in the leaves, shoots and roots of sweet potato plants infected with fusarium solani f. sp. batatas (fsb) or treated by its culture filtrate. the accumulation of aba occurred firstly in the roots, but the highest concentration was in the shoots. the endogenous ga(1/3) concentration of leaves, shoots and roots of infected sweet potato kept at markedly low levels during the period of experiments.the aba concentration of sweet potato seedlings inc ... | 2007 | 17675755 |
| fusarium solani species complex isolates conspecific with fusarium solani f. sp. cucurbitae race 2 from naturally infected human and plant tissue and environmental sources are equally virulent on plants, grow at 37 degrees c and are interfertile. | in a previous taxonomic study based on multilocus sequencing of fusarium from clinical specimens and hospital environments, the most common lineage was fusarium solani species complex group 1 (fssc 1) which is conspecific with f. solani f. sp. cucurbitae race 2, a pathogen of cucurbit fruits. the aims of our study were to determine if clinical and environmental isolates of fssc 1 are plant pathogens and members of the same biological species as cucurbit isolates, and to determine if all isolates ... | 2007 | 17686017 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| antimicrobial activity of methanol extract of origanum majorana l. (sweet marjoram). | in-vitro microbicidal activity of the methanol extract of origanum majorana l. was tested against seven fungi (fusarium solani, candida albicans, aspergillus niger, a. parasiticus, rhizopus oryzae, rhizoctonia otyzae-sativae and altemaria brassicicola) and six bacteria (bacillus subtilis, b. megaterium, escherichia coil, proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus). the methanol extract of o. majorana can be used as an effective herbal protectant against different pathogen ... | 2007 | 17718003 |
| ulcerous lesions disclosing cutaneous infection with fusarium solani. | fusarium spp. are emergent opportunistic moulds capable of producing life-threatening diseases, usually in immunocompromised hosts. when they infect immunocompetent patients, they usually cause localized diseases. we describe here a case of cutaneous fusariosis in a patient with a medical history of diabetes mellitus and non-hodgkin's lymphoma who presented with two painful lesions on her left leg. the diagnosis was made on the basis of histopathological findings and skin biopsy culture. the les ... | 2007 | 17721650 |
| prevention of fungal infection of plants by specific inhibition of cutinase. | specific antibodies prepared against cutinase from fusarium solani pisi and diisopropylfluorophosphate, a potent inhibitor of this enzyme, prevented infection of the host (pea epicotyl) by this organism, without affecting the viability of the spores. this finding shows that enzymatic penetration of cuticle is involved in pathogenesis. | 1979 | 17758793 |
| alkali bees: response of adults to pathogenic fungi in brood cells. | female alkali bees (nomia melanderi) opened sealed cells containing brood infested with aspergillus flavus, a. tamarii, fusarium solani, rhizopus sp., or mucor sp. and filled them with compact soil, thus reducing fungus sporulation. such awareness of the condition of sealed brood is hitherto unknown among solitary bees. | 1969 | 17770861 |
| antifungal activity of chitinases from trichoderma aureoviride dy-59 and rhizopus microsporus vs-9. | two chitinolytic fungal strains, trichoderma aureoviride dy-59 and rhizopus microsporus vs-9, were isolated from soil samples of korea and vietnam, respectively. dy-59 and vs-9 crude chitinases secreted by these fungi in the 0.5% swollen chitin culture medium had an optimal ph of 4 and the optimal temperatures of 40 degrees c and 60 degrees c, respectively. enzymatic hydrolysis products from crab swollen chitin were n-acetyl-beta-d-glucosamine (glcnac) by dy-59 chitinase, and glcnac and n, n'-di ... | 2008 | 17896135 |
| antifungal efficacy of voriconazole, itraconazole and amphotericin b in experimental fusarium solani keratitis. | to evaluate the efficacy of topical voriconazole in an experimental rabbit model of fusarium keratitis. | 2008 | 17912543 |
| sewage and community shower drains are environmental reservoirs of fusarium solani species complex group 1, a human and plant pathogen. | in two recent studies, clinical isolates in the fusarium solani species complex (fssc) were sequenced; one of the most common lineages was fssc group 1 (fssc 1), a phylogenetic species that is synonymous with f. solani f. sp. cucurbitae race 2, a pathogen of cucurbit fruits. fssc 1 was also identified in sink and shower drains in two hospitals. the environmental sources of fssc 1 are important for understanding the epidemiology of both human and plant diseases caused by this organism. fssc 1 was ... | 2008 | 17916080 |
| comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis. | the activity of various lipases was compared, in both free and immobilized forms, using the kinetics of the hydrolysis reaction of p-nitrophenyl butyrate, which was followed with in situ uv/vis diode array spectrophotometry. several enzymes were used to catalyze the reaction, namely candida antarctica lipase b and fusarium solani pisi cutinase wildtype and three single-mutation variants. the enzymes were tested in three different forms: free, immobilized as cross-linked aggregates and supported ... | 2008 | 17940805 |
| hypotin, a novel antipathogenic and antiproliferative protein from peanuts with a sequence similar to those of chitinase precursors. | a protein designated hypotin, with both antifungal and antibacterial activity, was isolated from peanut (arachis hypogaea) seeds. the isolation procedure included extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, ion chromatography, and gel filtration. the protein exhibited a molecular mass of 30.4 kda in sds-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions, indicating that it is a monomeric protein. its n-terminal sequence ... | 2007 | 17979231 |
| modulation of cutinase stability and structure by phospholipid detergents. | fusarium solani pisi cutinase hydrolyses triglycerides of different lengths. here we show that micelle-forming short-chain (c6-c9) phospholipids significantly reduce cutinase stability (both below and above the critical micelle concentration cmc) and rates of folding (only above cmc), trapping cutinase in an inactive state which only regains activity over hours to days, rather than the few seconds required for refolding in the absence of detergent. destabilization decreases with increasing chain ... | 2007 | 17980683 |
| toll-like receptors (tlrs) expression and function in response to inactivate hyphae of fusarium solani in immortalized human corneal epithelial cells. | to evaluate the role of toll-like receptors (tlrs) in host responses to fusarium solani by the use of cultured immortalized human corneal epithelial cells (hcec) and to determine whether inactive hyphal fragments can induce an antifungal response in these cells. | 2007 | 17982419 |
| fragrance release from the surface of branched poly (amide)s. | enzymes are powerful tools in organic synthesis that are able to catalyse a wide variety of selective chemical transformations under mild and environmentally friendly conditions. enzymes such as the lipases have also found applications in the synthesis and degradation of polymeric materials. however, the use of these natural catalysts in the synthesis and the post-synthetic modification of dendrimers and hyperbranched molecules is an application of chemistry yet to be explored extensively. in th ... | 2005 | 18007278 |
| deep cutaneous infection by fusarium solani in a healthy child. | 2007 | 18021857 | |
| isolation and biochemical characterization of an antifungal peptide from amaranthus hypochondriacus seeds. | an antifungal peptide, ay-amp, was isolated from amaranthus hypochondriacus seeds by acidic extraction and then purified by reverse-phase high-pressure liquid chromatography. the molecular mass of this peptide, as determined by mass spectrometry, is 3184 da. the peptide belongs to the superfamily of chitin-binding proteins, containing a single cysteine/glycine-rich chitin-binding domain, and it was found that ay-amp degrades chitin. ay-amp inhibits the growth, at very low doses, of different pat ... | 2007 | 18031007 |
| role of ethylene in the protection of tomato plants against soil-borne fungal pathogens conferred by an endophytic fusarium solani strain. | an endophytic fungal isolate (fs-k), identified as a fusarium solani strain, was obtained from root tissues of tomato plants grown on a compost which suppressed soil and foliar pathogens. strain fs-k was able to colonize root tissues and subsequently protect plants against the root pathogen fusarium oxysporum f.sp. radicis-lycopersici (forl), and elicit induced systemic resistance against the tomato foliar pathogen septoria lycopersici. interestingly, attenuated expression of certain pathogenesi ... | 2007 | 18048373 |
| in vitro interactions of antifungal agents against clinical isolates of fusarium spp. | the in vitro activities of amphotericin b (amb), itraconazole (itc), voriconazole (vcz) and terbinafine (tbf) alone and in the combinations amb+vcz, tbf+itc and tbf+vcz were evaluated against 29 clinical isolates of fusarium spp. (15 fusarium solani, 7 fusarium oxysporum, 2 fusarium decemcellulare, 2 fusarium dimerum and 3 other fusarium spp.). minimum inhibitory concentrations were determined using the method of the clinical and laboratory standards institute and the interaction activity was ca ... | 2008 | 18054469 |
| invasion of the french paleolithic painted cave of lascaux by members of the fusarium solani species complex. | a major fungal invasion was discovered in the prehistoric painted cave of lascaux in france in sep 2001. at least three species of the fusarium solani complex were isolated and identified with a portion of the translation elongation factor 1alpha gene (ef-1alpha), a portion of the nuclear large subunit rdna (lsu) and nuclear ribosomal intergenic spacer region (its). this study represents the first time that fusarium species have been reported from a cave containing prehistoric paintings. signifi ... | 2007 | 18065003 |
| [the roles of gelatinases in pathological changes of fungal keratitis in experimental rabbits]. | to study the roles of gelatinases, including matrix metalloproteinase (mmp)-2 and mmp-9, in pathological changes of fungal keratitis in rabbits. | 2007 | 18070528 |
| properties and applications of microbial d-amino acid oxidases: current state and perspectives. | d: -amino acid oxidase (daao) is a biotechnologically relevant enzyme that is used in a variety of applications. daao is a flavine adenine dinucleotide-containing flavoenzyme that catalyzes the oxidative deamination of d-isomer of uncharged aliphatic, aromatic, and polar amino acids yielding the corresponding imino acid (which hydrolyzes spontaneously to the alpha-keto acid and ammonia) and hydrogen peroxide. this enzymatic activity is produced by few bacteria and by most eukaryotic organisms. i ... | 2008 | 18084756 |
| on the mechanism of enzyme action; the influence of a pigment from fusarium solani d2 purple on the composition of fats formed in fusaria. | 1949 | 18108937 | |
| on the mechanism of enzyme action; solanione a pigment from fusarium solani d2 purple. | 1949 | 18131427 | |
| rational design of a new one-step purification strategy for candida antarctica lipase b by ion-exchange chromatography. | a fast and efficient one-step method for purification of lipase b from candida antarctica by ion-exchange chromatography was developed by rational design. the electrostatic properties of the enzyme were calculated and validated by isoelectric focusing and measurement of the titration curve. c. antarctica lipase b shows an unusual ph profile with a broad isoelectric region from ph 4 to 8. at ph 3 c. antarctica lipase b can be bound to a cation-exchange chromatography column and was purified to ho ... | 2008 | 18154980 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| occupational hypersensitivity pneumonitis due to molds in an onion and potato sorter. | a 42-year-old female non-smoking onion and potato sorter developed work-related shortness of breath, cough, fatigue and flu-like symptoms. the diagnosis of hypersensitivity pneumonitis was based on patchy infiltrates in both lungs on high resolution computed tomography and lymphocytosis of 71% in a bronchoalveolar lavage sample with a cd4/cd8 ratio of 0.4. exposure cessation and initial corticosteroid therapy resulted in complete recovery. igg antibodies to penicillium species and fusarium solan ... | 2008 | 18161826 |
| interactions between glyphosate and autochthonous soil fungi surviving in aqueous solution of glyphosate. | the survival of autochthonous fungi in soil treated with 1mm aqueous solution of glyphosate was investigated. significant differences in the total number of fungi in the studied objects were observed, and additionally significant qualitative changes were encountered. the dominating group of fungi belonged to genus fusarium: fusarium solani h30, fusarium solani h50 and fusarium oxysporum h80. interactions between the isolated strains of fungi and varying concentrations of glyphosate were determin ... | 2008 | 18177917 |
| activities of voriconazole, itraconazole and amphotericin b in vitro against 590 moulds from 323 patients in the voriconazole phase iii clinical studies. | fungal pathogens from the voriconazole trials were identified and tested for susceptibility at two reference laboratories. | 2008 | 18222957 |
| fungal root endophytes from natural vegetation in mediterranean environments with special reference to fusarium spp. | surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal and inland soils, both sandy soils and salt marshes) under either water or salt stress in the alicante province (southeast spain). all plant species examined were colonized by endophytic fungi. a total of 1830 fungal isolates were obtained and identified by morphological and molecular [internal transcribed spacer (its) and translation elongation factor-1alpha gene region (tef ... | 2008 | 18248439 |
| antifungal susceptibility profile of clinical fusarium spp. isolates identified by molecular methods. | to analyse the susceptibility pattern of a collection of fusarium clinical isolates. | 2008 | 18263569 |
| protein structure and dynamics in ionic liquids. insights from molecular dynamics simulation studies. | we present in this work the first molecular simulation study of an enzyme, the serine protease cutinase from fusarium solani pisi, in two ionic liquids (ils): 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][pf6]) and 1-butyl-3-methylimidazolium nitrate ([bmim][no(3)]). we tested different water contents in these ils at room temperature (298 k) and high temperature (343 k), and we observe that the enzyme structure is highly dependent on the amount of water present in the il media. we show ... | 2008 | 18266354 |
| amphotericin b formulations variably enhance antifungal activity of human neutrophils and monocytes against fusarium solani: comparison with aspergillus fumigatus. | lipid formulations of amphotericin b (ambf) are widely used in the treatment of life-threatening infections caused by aspergillus fumigatus and fusarium solani. we aimed to compare the immunomodulatory effects of four ambf, deoxycholate (damb), liposomal (lamb), lipid complex (ablc) and colloidal dispersion (abcd), on the oxidative antifungal activities of human neutrophils (pmns) and monocytes (mncs) against hyphae of a. fumigatus and f. solani. | 2008 | 18272514 |
| orientation of cutinase adsorbed onto pmma nanoparticles probed by tryptophan fluorescence. | the fluorescence of the single tryptophan (trp69) of cutinase from fusarium solani pisi, free in aqueous solution and adsorbed onto the surface of poly(methyl methacrylate) (pmma) latex particles, was studied at phs of 4.5 and 8.0. the monodisperse pmma particles (d=106.0+/-0.1 nm) were coated with a quite compact monolayer of cutinase at both ph values. the trp decay curve of the folded free cutinase in solution can only be fitted with a sum of four exponentials with lifetimes of 0.05, 0.3-0.4, ... | 2008 | 18311968 |
| expression in escherichia coli, purification, refolding and antifungal activity of an osmotin from solanum nigrum. | abstract: | 2008 | 18334031 |
| [non-dermatophytic moulds: onychomycosis in four patients infected with the human immunodeficiency virus]. | patients infected with human immunodeficiency virus (hiv) are a risk group for onychomycosis, fungal infections caused mainly by dermatophytes and yeast. however, non-dermatohytic moulds are becoming common agents for nail infections in this population of patients. we report four cases of onychomycosis caused by non-dermatophytic moulds (aspergillus niger, scytalidium hyalinum, scytalidium dimidiatum and fusarium solani) in patients infected with hiv from recife, pernambuco, brazil. onychomicosi ... | 2008 | 18338928 |
| antifungal and antibacterial activities of taxus wallichiana zucc. | current study was undertaken to evaluate the in vitro antifungal and antibacterial potential of methanol extract and subsequent fractions obtained after partitioning in organic solvents with variable polarity of the aerial parts of the tree taxus wallichiana zucc. traditionally, this plant is often used in folk medicines in pakistan for treating microbial infections. in order to rationalize the traditional use, methanol extracts of leaf, bark, and heartwood of taxus wallichiana zucc. were tested ... | 2008 | 18343912 |
| fungal removal of gaseous hexane in biofilters packed with poly(ethylene carbonate) pine sawdust or peat composites. | the removal of volatile organic compounds (voc) in biofilters packed with organic filter beds, such as peat moss (pm) and pine sawdust (ps), frequently presents drawbacks associated to the collapse of internal structures affecting the long-term operation. poly(ethylene ether carbonate) (peec) groups grafted to these organic carriers cross linked with 4,4'-methylenebis(phenylisocyanate) (mdi) permitted fiber aggregation into specific shapes and with excellent hexane sorption performance. modified ... | 2008 | 18351679 |
| [diversity of facultatively anaerobic microscopic mycelial fungi in soils]. | the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ... | 2008 | 18365728 |
| fusarium solani in the post-transplant patient: an unusual fungus. | the incidence of fungal infections in post-transplant patients ranges from 2% to 50%, and more uncommon pathogens are being isolated. earlier, the fungi seen were candida, aspergillus, and cryptococcus; and of late, histoplasma, coccidiodomycoses, and dematiaceous fungi are seen. in this article, a patient with invasive fusariosis is presented, and literature of this uncommon fungus is reviewed. | 2008 | 18372269 |
| antifungal activity of coumarins. | the antifungal activity of 40 coumarins was tested against the fungal strains: candida albicans (atcc 14053), aspergillus fumigatus (atcc 16913) and fusarium solani (atcc 36031), using the broth microdilution method. osthenol showed the most effective antifungal activity among all the compounds tested, with a mic value of 125 microg/ml for fusarium solani and 250 micro/ml for candida albicans and aspergillus fumigatus. the antifungal potential of this prenylated coumarin can be related to the pr ... | 2008 | 18386483 |
| expression of toll-like receptors in the fusarium solani infected cornea. | to investigate the expression of toll-like receptors (tlrs) in the human cornea with fusarium solani (f. solani) keratitis. | 2008 | 18398706 |
| enzymatic hydrolysis of ptt polymers and oligomers. | oligomers and polymers (film, fabrics) of the linear aromatic polyester poly(trimethylene terephthalate) (ptt) were treated with polyesterases from thermomyces lanuginosus, penicillium citrinum, thermobifida fusca and fusarium solani pisi. the cutinase from t. fusca was found to release the highest amounts of hydrolysis products from ptt materials and was able to open and hydrolyse a cyclic ptt dimer according to rp-hplc-uv detection. in contrast, the lipase from t. lanuginosus also showed activ ... | 2008 | 18405994 |
| improving activity and stability of cutinase towards the anionic detergent aot by complete saturation mutagenesis. | cutinase is an enzyme suitable for detergent applications as well as for organic synthesis in non-aqueous solvents. however, its inactivation in the presence of anionic surfactants is a problem which we have addressed by creating a complete saturation library. for this, the cutinase gene from fusarium solani pisi was mutated to incorporate all 19 possible amino acid exchanges at each of the 214 amino acid positions. the resulting library was screened for active variants with improved stability i ... | 2008 | 18424821 |
| enhancing the thermal stability of lipases through mutagenesis and immobilization on zeolites. | the hydrolysis reaction of p-nitrophenyl butyrate catalyzed by lipases was followed with in situ uv/vis diode array spectrophotometry. five enzymes - candida antarctica lipase b and fusarium solani pisi cutinase wild-type and three single-mutation variants - were tested as catalysts in homogeneous conditions and immobilized on zeolite nay, on a polyacrylate support and as cross-linked aggregates. using deconvolution techniques and kinetic modeling, the thermal stability of the different biocatal ... | 2009 | 18443829 |
| dual elicitation for improved production of withaferin a by cell suspension cultures of withania somnifera. | high yielding transformed callus culture of w. somnifera was established by infecting hypocotyls with agrobacterium tumefaciens mtcc-2250. maximum withaferin a content of 0.0875 mg/g dry cell weight and transformation efficiency of 80% were obtained. confirmation of transformation was done on the basis of the presence of the ags gene by using polymerase chain reaction. various abiotic elicitors (arachidonic acid, methyl jasmonate, calcium chloride, and copper sulfate) and biotic elicitors (cell ... | 2008 | 18449479 |
| screening of tropical fungi producing polyethylene terephthalate-hydrolyzing enzyme for fabric modification. | microfungi were selectively isolated for production of polyethylene terephthalate (pet) fiber-degrading enzymes potentially to be used to modify the surface of polyester fabric. a range of fungi were isolated from plant surfaces and soil samples using a polycaprolactone (pcl) plate-clearing assay technique, and screened for cutinolytic esterase (cutinase) activity. twenty-two of 115 isolates showed clearing indicating the production of cutinase. the ability of the fungi to produce cutinase in mi ... | 2008 | 18449587 |
| quantification of fusarium solani f. sp. glycines isolates in soybean roots by colony-forming unit assays and real-time quantitative pcr. | fusarium solani f. sp. glycines (fsg; syn. f. virguliforme akoi, o'donnell, homma & lattanzi) is a soil-borne fungus that infects soybean roots and causes sudden death syndrome (sds), a widespread and destructive soybean disease. the goal of this study was to develop and use a real-time quantitative polymerase chain reaction (qpcr) assay to compare the accumulation of genomic dna among 30 fsg isolates in inoculated soybean roots. isolates differed significantly (p < or = 0.05) in their dna accum ... | 2008 | 18461301 |
| development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis. | aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only to candida species as a cause of invasive fungal infections in immunocompromised humans. current immunodiagnostic tests for invasive aspergillosis (ia) are based on the detection of circulating galactomannan (gm) in a patient's serum by using a rat monoclonal antibody (mab), eb-a2, that binds to tetra (1-->5)-beta-d-galactofuranoside, the immunodominant epitope in gm. the potential cross-reactivity of mab eb-a2 with ... | 2008 | 18463222 |
| probing the microenvironment of sol-gel entrapped cutinase: the role of added zeolite nay. | cutinase, an esterase from fusarium solani pisi, was immobilized in sol-gel matrices of composition 1:5 tetramethoxysilane (tmos):n-alkyltrimethoxysilane (n-alkyltms). fluorescence spectroscopy using the single tryptophan (trp-69) residue of cutinase as a probe revealed that the polarity of the matrices decreased as their hydrophobicity increased up to the tmos/n-butyltms pair, which correlates with an increase in cutinase activity. fluorescence emission was suppressed (a higher than two orders ... | 2008 | 18490069 |
| combined therapy in treatment of murine infection by fusarium solani. | we evaluated the efficacy of voriconazole, amphotericin b and micafungin alone and combined in a murine model of disseminated infection by fusarium solani. | 2008 | 18495651 |
| late-onset infections after lasik. | to report two cases of infectious keratitis that developed 2 and 6 years after lasik. | 2008 | 18500093 |
| caspofungin-mediated beta-glucan unmasking and enhancement of human polymorphonuclear neutrophil activity against aspergillus and non-aspergillus hyphae. | we investigated whether caspofungin and other echinocandins have immune-enhancing properties that influence human polymorphonuclear neutrophil (pmn)-mediated mold hyphal damage. | 2008 | 18500936 |
| biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant aot. | the reactivity, stability and unfolding of wild-type (wt) fusarium solani pisi cutinase and l153q, s54d and t179c variants were studied in the absence and presence of the dioctyl sulfosuccinate sodium salt (aot) surfactant. in the absence of surfactant the s54d variant catalytic activity is similar to that of the wt cutinase, whereas l153q and t179c variants show a lower activity. aot addition induces an activity reduction for wt cutinase and its variants, although for low aot concentrations a s ... | 2008 | 18501723 |
| a pseudo-outbreak of fusarium solani in an intensive care unit associated with bronchoscopy. | 2008 | 18511154 | |
| molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the fusarium solani species complex. | members of the species-rich fusarium solani species complex (fssc) are responsible for approximately two-thirds all fusarioses of humans and other animals. in addition, many economically important phytopathogenic species are nested within this complex. due to their increasing clinical relevance and because most of the human pathogenic and plant pathogenic fssc lack latin binomials, we have extended the multilocus haplotype nomenclatural system introduced in a previous study (d. c. chang, g. b. g ... | 2008 | 18524963 |
| production of paclitaxel by fusarium solani isolated from taxus celebica. | a fungus was isolated from the stem cuttings of taxus celebica, which produced paclitaxel in liquid-grown cultures. the fungus was identified as fusarium solani based on colony characteristics, morphology of conidia and the 26s rdna sequence. paclitaxel was identified by chromatographic and spectroscopic comparison with authentic paclitaxel and its cytotoxic activity towards jurkat cells in vitro. | 2008 | 18535360 |
| chemical composition and antifungal activity of essential oil and various extract of silene armeria l. | the aims of this study were to examine the chemical composition of the essential oil isolated from the floral parts of silene armeria l. by hydrodistillation, and to test the efficacy of essential oil and various leaf extracts (n-hexane, chloroform, ethyl acetate and methanol) as an antifungal potential. the gc-ms analysis determined that 28 compounds, which represented 89.03% of total oil, were present in the oil containing mainly 1-butene, methylcyclopropane, 2-butene and caryophyllene oxide. ... | 2008 | 18538562 |
| the use of a hydrophobic resin as a product reservoir in steroid transformations. | particles of the hydrophobic resin polydimethylsiloxane were found to preferentially accumulate steriods on the basis of their hydrophobicity. thus, the resin selectively sorped the steroid products resulting from the transformation of diosgenin by nocardia rhodochrous, with the result that higher yields of the later biotransformation product, 1-dehydrodiosgenone, and lower yields of the first product, diosgenone, were obtained than in the absence of resin. furthermore, steroids accumulated by t ... | 1985 | 18553742 |
| immunological diversity within a family of cutinase-like proteins of mycobacterium tuberculosis. | secreted proteins of mycobacterium tuberculosis play key roles in the assembly of the mycobacterial cell wall, with many being major targets of the host immune response. to date, meaningful characterization of a significant proportion of this important group of proteins is lacking. among the group of putatively secreted proteins of m. tuberculosis are 7 cutinase-like proteins (clp), not previously characterized in terms of their immunogenicity or vaccine protective efficacy. although the clp var ... | 2008 | 18565629 |
| myd88 regulation of fusarium keratitis is dependent on tlr4 and il-1r1 but not tlr2. | the fungal pathogens fusarium solani and fusarium oxysporum cause severe corneal disease in the united states and worldwide and were the causative organisms in a recent outbreak of contact lens-associated keratitis. to characterize innate immunity in fusarium keratitis, we developed a murine model in which conidia are injected into the corneal stroma. immunocompetent c57bl/6 mice rapidly developed severe corneal opacification associated with neutrophil infiltration and clearance of fusarium hyph ... | 2008 | 18566426 |
| gas phase transesterification reactions catalyzed by lipolytic enzymes. | porcine pancreatic lipase and fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrates and products are in a gaseous form. in this type of system, enzyme activity was found to depend essentially on the water activity (a(w)) of the enzyme preparation. | 1992 | 18600970 |
| mold species in dust from the international space station identified and quantified by mold-specific quantitative pcr. | dust was collected over a period of several weeks in 2007 from hepa filters in the u.s. laboratory module of the international space station (iss). the dust was returned on the space shuttle atlantis, mixed, sieved and the dna was extracted. using a dna-based method called mold-specific quantitative pcr (msqpcr), 39 molds were measured in the dust. potential opportunistic pathogens aspergillus flavus and aspergillus niger and potential moderate toxin producers penicillium chrysogenum and penicil ... | 2008 | 18602989 |
| synthesis of organometallic-based biologically active compounds: in vitro antibacterial, antifungal and cytotoxic properties of some sulfonamide incorporated ferrocences. | sulfonamides incorporated ferrocene (sif) have been synthesized by the condensation reaction of sulfonamides (sulfanilamide, sulfathiazole or sulfamethaxazole) with 1,1'-diacetylferrocene. the synthesized compounds (sif(1)-sif(4)) have been characterized by their physical, spectral and analytical properties and have been screened for their in vitro antibacterial properties against pathogenic bacterial strains e.g., escherichia coli, pseudomonas aeruginosa, bacillus subtilis staphylococcus aureus ... | 2009 | 18608785 |
| in vitro antifungal and antibacterial activities of extracts of galium tricornutum subsp. longipedunculatum. | the aim of this study was to evaluate the antimicrobial activity of crude ethanolic extracts and fractions of the ariel parts and the fruits of galium tricornutum subsp. longipedunculatum, traditionally used in northern areas of pakistan for treating microbial infections of skin. extracts and their fractions were tested against six bacteria and six fungal strains using the hole diffusion method and macrodilution method. all extracts and fractions possessed significant antimicrobial effect. four ... | 2009 | 18608788 |
| synthesis of fatty acid esters by a recombinant cutinase in reversed micelles. | fusarium solani pisi recombinant cutinase, solubilized in aot/isooctane-reversed micelles, was used to catalyze the esterification of fatty acids with aliphatic alcohols. some relevant parameters for the enzyme activity such as ph, w(o) (water/surfactant molar ratio), temperature, and substrate concentration were optimized. maximal specific activity was obtained for hexanol. the cutinase showed selectivity for short-chain fatty acids. the stability of the microencapsulated cutinase was investiga ... | 1993 | 18613016 |
| working at controlled water activity in a continuous process: the gas/solid system as a solution. | fusarium solani cutinase and candida cylindracea lipase were used to catalyze a transesterification reaction in a continuous gas/solid bioreactor. in this system, a solid phase composed of a packed enzymatic preparation was continuously percolated with carrier gas which fed substrate and removed reaction products simultaneously. different conditions of immobilization were used and compared to the results obtained with a nonsupported enzyme. the enzymatic activity was found to be highly dependent ... | 1995 | 18623231 |
| use of multiple methods for genotyping fusarium during an outbreak of contact lens associated fungal keratitis in singapore. | in singapore, an outbreak of fungal keratitis caused by members of the fusarium solani species complex (fssc) was identified in march 2005 to may 2006 involving 66 patients. epidemiological investigations have indicated that improper contact lens wear and the use of specific contact lens solutions were risk factors. | 2008 | 18627616 |
| fungistatic activity of iron-free bovin lactoferrin against several fungal plant pathogens and antagonists. | lactoferrin (lf) is a member of the transferrin family of iron-binding glycoproteins. it is also a multifunctional protein of 80 kda that is synthesized by glandular epithelial cells and secreted into mucosal fluid. high levels of lf are present in colostrom and milk and low levels in tears, saliva, and gastrointestinal and reproductive secretions. data regarding the antifungal effects of lf are limited. studies have been performed on candida albicans, which demonstrated that lf inhibits the gro ... | 2008 | 18629710 |
| multiplex tandem pcr: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens. | we describe the first development and evaluation of a rapid multiplex tandem pcr (mt-pcr) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. the assay uses a short-cycle multiplex amplification, followed by 12 simultaneous pcrs which target the fungal internal transcribed spacer 1 (its1) and its2 region, elongation factor 1-alpha (ef1-alpha), and beta-tubulin genes to identify 11 fungal pathogens: candida albicans, candid ... | 2008 | 18632914 |
| kinetic studies of fusarium solani pisi cutinase used in a gas/solid system: transesterification and hydrolysis reactions. | fusarium solani cutinase supported onto chromosorb p was used to catalyze transesterification (alcoholysis) and hydrolysis on short volatile alcohols and esters in a continuous gas/solid bioreactor. in this system, a solid phase composed of a packed enzymatic preparation was continuously percolated with carrier gas which fed substrates and removed reaction products simultaneously. a kinetic study was performed under differential operating conditions in order to get initial reaction rates. the ef ... | 1997 | 18636604 |
| predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems. | a model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. this model accounts for both charge-independent and electrostatic effects. the determination of nonelectrostatic effects was based on the model of eiteman and gainer for uncharged solutes while the electrostatic contribution was computed using titra, a program that uses a continuum electrostatic model to treat charge interactions in proteins and considers the effect of ph and ionic stren ... | 1997 | 18636640 |
| persisting onychomycosis caused by fusarium solani in an immunocompetent patient. | we present a patient with a fusarium solani infection of both distal thumbnails. she suffered from basedow's disease but had no signs of reduced immunity. the patient declined antimycotic treatment and the infection was still verifiable after 5 years. our observation shows that a superficial f. solani infection may persist in immunocompetent patients without causing noteworthy discomfort. because a superficial f. solani infection may give origin to a systemic infection it should always be treate ... | 2009 | 18643888 |
| synthetic affinity ligands as a novel tool to improve protein stability. | cutinase from fusarium solani pisi is the model-system for a new approach to assess and enhance protein stability based on the use of synthetic triazine-scaffolded affinity ligands as a novel protein-stabilizing tool. the active site of cutinase is excluded from the main surface regions postulated to be involved in early protein's thermal unfolding events. hence, these regions are suitable targets for binding complementary affinity ligands with a potential stabilizing effect. a random solid-phas ... | 2009 | 18654989 |
| identification and characterization of bacterial cutinase. | cutinase, which exists in both fungi and bacteria, catalyzes the cleavage of the ester bonds of cutin. fungal cutinases have been extensively studied, however, reports on bacterial cutinases have been limited due to the lack of knowledge concerning the identity of their open reading frames. in the present study, the cutinase from thermobifida fusca was induced by cutin and purified to homogeneity by following p-nitrophenyl butyrate hydrolyzing activity. peptide mass fingerprinting analysis of th ... | 2008 | 18658138 |
| microbial community analysis of field-grown soybeans with different nodulation phenotypes. | microorganisms associated with the stems and roots of nonnodulated (nod(-)), wild-type nodulated (nod(+)), and hypernodulated (nod(++)) soybeans [glycine max (l.) merril] were analyzed by ribosomal intergenic transcribed spacer analysis (risa) and automated risa (arisa). risa of stem samples detected no bands specific to the nodulation phenotype, whereas risa of root samples revealed differential bands for the nodulation phenotypes. pseudomonas fluorescens was exclusively associated with nod(+) ... | 2008 | 18658280 |
| in-vitro antibacterial, antifungal activity of some transition metal complexes of thiosemicarbazone schiff base (hl) derived from n4-(7'-chloroquinolin-4'-ylamino) thiosemicarbazide. | the synthetic, spectroscopic, and biological studies of cu(ii), ni(ii), zn(ii), co(ii), mn(ii), fe(iii) and cr(iii) complexes of n(4)-(7'-chloroquinolin-4'-ylamino)-n(1)-(2-hydroxy-benzylidene)thiosemicarbazone (hl) obtained by the reaction of n(4)-(7'-chloroquinolin-4'-ylamino)thiosemicarbazide with 2-hydroxybenzaldehyde. the structures of the complexes were determined on the basis of the elemental analyses, spectroscopic data (ir, electronic, (1)h and (13)c nmr and mass spectra) along with mag ... | 2008 | 18665996 |
| first isolation of a novel thermostable antifungal peptide secreted by aspergillus clavatus. | a novel antifungal peptide produced by an indigenous fungal strain (vr) of aspergillus clavatus was purified. the antifungal peptide was enriched in the supernatant after heat treatment at 70 degrees c. the thermostable character was exploited in the first purification step, as purified peptide was obtained after ultrafiltration and reverse phase-hplc on c18 column application. the purified peptide named "acafp" for a. clavatus antifungal peptide, has molecular mass of 5773da determined by maldi ... | 2008 | 18687373 |
| activities of antifungal agents against yeasts and filamentous fungi: assessment according to the methodology of the european committee on antimicrobial susceptibility testing. | we compared the activities of antifungal agents against a wide range of yeasts and filamentous fungi. the methodology of the european committee on antimicrobial susceptibility testing (eucast) for yeasts and spore-forming molds was applied; and a total of 349 clinical isolates of candida spp., other yeast species, aspergillus spp., and nondermatophyte non-aspergillus spp. were investigated. the average geometric mean (gm) of the mics of the various drugs for candida spp. were as follows: amphote ... | 2008 | 18694949 |
| following multi-component reactions in liquid medium using spectral band-fitting techniques. | the kinetics of the hydrolysis reaction of p-nitrophenyl butyrate catalyzed by a single-site mutant of fusarium solani pisi cutinase, supported on zeolite nay by adsorption, was followed using ultraviolet-visible (uv/vis) in situ diode array spectrophotometry. spectral band-fitting techniques allowed the use of in situ diode array spectrophotometry to obtain quantitative data on the concentrations of the various species present in an aqueous reaction medium even in the presence of the solid cata ... | 2008 | 18702868 |
| ctf1, a transcriptional activator of cutinase and lipase genes in fusarium oxysporum is dispensable for virulence. | cutinolytic enzymes are secreted by fungal pathogens attacking the aerial parts of the plant, to facilitate penetration of the outermost cuticular barrier of the host. the role of cutinases in soil-borne root pathogens has not been studied thus far. here we report the characterization of the zinc finger transcription factor ctf1 from the vascular wilt fungus fusarium oxysporum, a functional orthologue of ctf1alpha that controls expression of cutinase genes and virulence in the pea stem pathogen ... | 2008 | 18705871 |
| determination of in vitro susceptibility of ocular fusarium spp. isolates from keratitis cases and comparison of clinical and laboratory standards institute m38-a2 and e test methods. | we evaluated the susceptibility of 85 fusarium spp. isolates from cases of fungal keratitis with 8 antifungal drugs using the standard clinical and laboratory standards institute broth microdilution and e test methods. members of the fusarium solani species complex showed consistently higher mics to the triazole drugs itraconazole, voriconazole, and posaconazole than did members of other species complexes (fusarium oxysporum and other minor species). high mics to amphotericin b, natamycin, and e ... | 2008 | 18707841 |