Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| two novel secreted ferritins involved in immune defense of chinese mitten crab eriocheir sinensis. | as a principal extracellular iron storage molecule, secreted ferritin plays an important role in the iron-withholding strategy of innate immunity. in this study, two novel secreted ferritins were identified from chinese mitten crab eriocheir sinensis (designated as esfer-1 and esfer-2) by rapid amplification of cdna ends (race) approaches and expressed sequence tag (est) analysis. the full-length cdnas of esfer-1 and esfer-2 were of 1278 and 1595 bp, respectively, both containing a putative iron ... | 2010 | 20045469 |
| an inhibitor kappab homologue from bay scallop argopecten irradians. | ikappab is an important member of nf-kappab pathway in the innate immune system. in the present study, the full-length cdna sequence encoding ikappab protein (designated aiikappab) was isolated from bay scallop argopecten irradians. the complete sequence of aiikappab cdna containing a 5' untranslated region (utr) of 237 bp, a 3' utr of 1023 bp with a poly (a) tail, and an open reading frame (orf) of 1086 bp encoding a polypeptide of 361 amino acids with the predicted molecular weight of 39.9 kda ... | 2010 | 20074646 |
| determination of sulphate-reducing bacteria based on vancomycin-functionalized magnetic nanoparticles using a modification-free quartz crystal microbalance. | a fast, sensitive and reliable quartz crystal microbalance (qcm) biosensor is described for the selective detection of the marine pathogenic sulphate-reducing bacterium (srb), d. desulfotomaculum. based on the amplification of the response of vancomycin-functionalized magnetic nanoparticles (van-mnps), under an external magnetic field, the bacteria-mnps conjugates attach to the surface of an au electrode. the qcm biosensor gave a distinct response to the vancomycin-sensitive, d. desulfotomaculum ... | 2010 | 20079624 |
| expression of antimicrobial peptides from hyas araneus haemocytes following bacterial challenge in vitro. | circulating haemocytes play major roles in the host defense reactions of decapods, including the synthesis and release of antimicrobial peptides (amps). unlike the amps from insects, those in decapods are constitutively expressed. this study aims to establish primary cell cultures of the three main haemocyte types in hyas araneus haemocytes, and to measure the in vitro expression of amp genes in the cells following microbial challenge. the haemocyte populations were separated on percoll gradient ... | 2010 | 20083137 |
| selection of candidate probionts by two different screening strategies from atlantic cod (gadus morhua l.) larvae. | two primary selection criteria were used to collect a pool of nearly 500 candidate probiotic bacteria from atlantic cod (gadus morhua l.) larvae, i.e. the dominant intestinal bacterial flora and isolates with antagonistic activity against vibrio anguillarum. bacteria were isolated from cod larvae from five rearing groups with variable rearing technologies. the bacteria were brought to pure culture and characterized phenotypically. based on properties such as uniqueness, dominance and fermentativ ... | 2010 | 20097491 |
| inactivation of vibrio anguillarum by attached and planktonic roseobacter cells. | the purpose of the present study was to investigate the inhibition of vibrio by roseobacter in a combined liquid-surface system. exposure of vibrio anguillarum to surface-attached roseobacters (10(7) cfu/cm(2)) resulted in significant reduction or complete killing of the pathogen inoculated at 10(2) to 10(4) cfu/ml. the effect was likely associated with the production of tropodithietic acid (tda), as a tda-negative mutant did not affect survival or growth of v. anguillarum. | 2010 | 20118354 |
| cloning and characterization of an invertebrate type lysozyme from venerupis philippinarum. | lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections and providing nutrition as digestion enzymes. in the present study, an invertebrate type lysozyme (denoted as vplyz) was identified from venerupis philippinarum haemocytes by cdna library and race approaches. the full-length cdna of vplyz consisted of 805 nucleotides with a canonical polyadenylation signal sequence aataaa and a polya tail, and an open-reading frame of 558bp encoding a polypept ... | 2010 | 20144728 |
| molecular characterization and expression of a crustin-like gene from chinese mitten crab, eriocheir sinensis. | crustin is a cysteine-rich antibacterial peptide which widely distributes within decapod crustaceans. in the present study, the cdna encoding crustin-like peptide (designated cruses) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches and expressed sequence tag analysis. the full-length cdna of cruses was of 796bp, containing a 5' untranslated region (utr) of 214bp, a 3' utr of 267bp with a poly(a) tail, and an open reading frame (or ... | 2010 | 20144896 |
| cloning and analysis of a ferritin subunit from turbot (scophthalmus maximus). | ferritin is an evolutionarily conserved protein that plays an important role in iron storage and detoxification. in this study, the gene encoding a ferritin h subunit homologue (smfer1) was cloned from turbot (scophthalmus maximus) and analyzed at the expression and functional levels. the open reading frame of smfer1 is 534 bp and preceded by a 5'-untranslated region that contains a putative iron regulatory element (ire). the deduced amino acid sequence of smfer1 shares extensive sequence identi ... | 2010 | 20152905 |
| antimicrobial activity in the tissues of atlantic cod (gadus morhua l.). | antimicrobial peptides (amp) are important components of the innate immune system in metazoans. they have been studied widely in several fishes, but little is known about these defence factors in atlantic cod, which is thought to have a less sophisticated adaptive immune system compared to other teleosts. the aim of the present study was to screen for potential amps in various tissues of atlantic cod and to examine their spectra of activity. acidic crude extracts were prepared from thirteen tiss ... | 2010 | 20153440 |
| three kazal-type serine proteinase inhibitors from the red swamp crayfish procambarus clarkii and the characterization, function analysis of hcpcspi2. | three kazal-type serine proteinase inhibitors, hcpcspi2, hppcspi3, and hppcspi4, with complete cdna sequences, were identified from a cdna library of the red swamp crayfish, procambarus clarkii. semi-quantitative rt-pcr shows that hcpcspi2 exists mainly in hemocytes while both hppcspi3 and hppcspi4 were detected in the hepatopancreas and the heart. homology comparison and phylogenic analysis indicate that hppcspi3 and hppcspi4 shared high identity and formed the same group, and both of them were ... | 2010 | 20170735 |
| disease susceptibility of salmon exposed to polybrominated diphenyl ethers (pbdes). | the health effects of the flame retardant polybrominated diphenyl ethers (pbdes) in fish are not well understood. to determine the potential effects of this ubiquitous contaminant class on fish health, juvenile subyearling chinook salmon (oncorhynchus tshawytscha) were fed a diet that reflected the pbde congeners found in the stomach contents of subyearling chinook salmon collected from the highly urbanized and industrialized lower willamette river in the columbia river basin of north america. t ... | 2010 | 20207027 |
| effects of anthraquinone extract from rheum officinale bail on the growth performance and physiological responses of macrobrachium rosenbergii under high temperature stress. | in order to study the effects of anthraquinone extract from rheum officinale bail on macrobrachium rosenbergii under high temperature stress, freshwater prawns were randomly divided into five groups: a control group was fed with basal diet, and four treatment groups fed with basal diet supplemented with 0.05%, 0.1%, 0.2%, and 0.4% anthraquinone extracts for 10 weeks, respectively. then, freshwater prawns were exposed to high temperature stress at 35 degrees c for 48h. the growth, changes in haem ... | 2010 | 20219682 |
| identification and molecular analysis of a novel c-type lectin from scophthalmus maximus. | c-type lectins are calcium-dependent carbohydrate-binding proteins that play important roles in innate immunity. in this study, a c-type lectin homologue (smlec1) was identified from turbot (scophthalmus maximus) and analyzed at expression and functional levels. the open reading frame of smlec1 is 504 bp, with a 5'-untranslated region (utr) of 101 bp and a 3'-utr of 164 bp. the deduced amino acid sequence of smlec1 shares 34%-38% overall identities with the c-type lectins of several fish species ... | 2010 | 20226863 |
| beta-actin is a useful internal control for tissue-specific gene expression studies using quantitative real-time pcr in the half-smooth tongue sole cynoglossus semilaevis challenged with lps or vibrio anguillarum. | beta-actin has been frequently used as an internal control (gene) or as a housekeeping gene to normalize the expression of the target gene(s) or mrna levels between different samples. however, the beta-actin expression has been shown to be influenced by the sample type and experimental conditions. if beta-actin could be used as a reference gene for the half-smooth tongue sole cynoglossus semilaevis remains ill-defined. here we evaluate the tissue-specific beta-actin gene expression pattern in c. ... | 2010 | 20227507 |
| presence of typical and atypical virulence genes in vibrio isolates belonging to the harveyi clade. | the study was aimed at investigating the presence of typical and atypical virulence genes in isolates belonging to the harveyi clade (vibrio harveyi and vibrio campbellii). | 2010 | 20345385 |
| aic1qdc-1, a novel gc1q-domain-containing protein from bay scallop argopecten irradians with fungi agglutinating activity. | the globular c1q-domain-containing (c1qdc) proteins are a family of versatile pattern recognition receptors via their globular c1q (gc1q) domain to bind various ligands including several pamps on pathogens. in this study, a new gc1q-domain-containing protein (aic1qdc-1) gene was cloned from argopecten irradians by rapid amplification of cdna ends (race) approaches and expressed sequence tag (est) analysis. the full-length cdna of aic1qdc-1 was composed of 733bp, encoding a signal peptide of 19 r ... | 2010 | 20346969 |
| a new c-type lectin (fclec5) from the chinese white shrimp fenneropenaeus chinensis. | c-type lectins are one family of pattern recognition receptors (prrs) that play important roles in innate immunity. in this work, cdna and genomic sequences for a new c-type lectin (fclec5) were obtained from the chinese white shrimp fenneropenaeus chinensis. fclec5 cdna contains an open reading frame of 1,008 bp and its genomic sequence is 1,137 bp with 4 exons and 3 introns. the predicted fclec5 protein contains a signal peptide and two carbohydrate recognition domains (crds). the n-terminal c ... | 2010 | 20349323 |
| unmethylated cpg oligodeoxynucleotides activate head kidney leukocytes of atlantic cod, gadus morhua. | bacterial dna and synthetic oligodeoxynucleotides (odns) that contain unmethylated cpg motifs are strong inducers of immune response in most mammalian organisms. the use of these synthetic cpg motifs in fish, particularly in salmonids and carp, resulted in the modulation of their immune system. however, much less is known in other species of fish such as gadoids including atlantic cod, gadus morhua. using head kidney (hk) leukocytes of cod in an in vitro study, we determined the effects of some ... | 2010 | 20349339 |
| characterization of chitinolytic bacteria and histological aspects of shell disease syndrome in european spiny lobsters (palinurus elephas) (fabricius 1787). | the present research reports the first description of shell disease syndrome in european spiny lobsters palinurus elephas (fabricius 1787), which occurred in an experimental aquaculture facility in sicily (italy). both bacterial characterization and histopathological examination of the exoskeleton at site of lesions was carried out. infected specimens showed tail fan erosions, and in one case uropod ulceration and complete loss of periods. identified species included: listonella anguillarum 50.5 ... | 2010 | 20361978 |
| broad-host-range plasmids for red fluorescent protein labeling of gram-negative bacteria for use in the zebrafish model system. | to observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (danio rerio), a series of plasmids was constructed for the red fluorescent protein (rfp) labeling of a variety of fish and human pathogens. the aim of this study was to create a collection of plasmids that would express rfp pigments both constitutively and under tac promoter regulation and that would be nontoxic and broadly transmissible to a variety of gram-negative b ... | 2010 | 20363780 |
| development of protection in rainbow trout (oncorhynchus mykiss, walbaum) to vibrio anguillarum following use of the probiotic kocuria sm1. | probiotic kocuria sm1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (oncorhynchus mykiss) fingerlings as dietary supplement at approximately 10(8) cells g(-1) for two weeks. the fish were challenged with vibrio anguillarum and innate immunity determined over the following 5 weeks. the probiotic-fed fish survived (mortalities = 10-28%; p < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of ko ... | 2010 | 20371293 |
| comparative susceptibility of turbot, halibut, and cod yolk-sac larvae to challenge with vibrio spp. | in intensive aquaculture systems, high mortalities are frequently observed during the early life stages of marine fish. the aim of this study was to investigate differences in the susceptibility of turbot scophthalmus maximus, halibut hippoglossus hippoglossus and cod gadus morhua to various strains of vibrio anguillarum (serotypes o1, o2alpha and o2beta), v salmonicida and v splendidus. the bath challenge experiments were performed using a multidish system, with 1 egg well-1. unchallenged eggs ... | 2010 | 20391910 |
| proteomic analysis of differentially expressed proteins in lymphoid organ of fenneropenaeus chinensis response to vibrio anguillarum stimulation. | to gain an insight into the function of shrimp lymphoid organ at protein level, we analyzed the proteome of lymphoid organ in healthy chinese shrimp fenneropenaeus chinensis (f. chinensis) through two-dimensional gel electrophoresis (2-de) based proteomic approach. a total of 95 spots representing 75 protein entries were identified by liquid chromatography tandem mass spectrometry (lc-ms/ms) with both online and in-house database. according to gene ontology (go) annotation of biological process, ... | 2010 | 20394824 |
| identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in venerupis philippinarum. | small heat shock proteins (shsps) encompass a widespread and diverse class of proteins with molecular chaperone activity. in the present study, two shsp isoforms (vpshsp-1 and vpshsp-2) were cloned from venerupis philippinarum haemocytes by rapid amplification of cdna ends (race) approaches. the expression profiles of these two genes under vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. the bacterial c ... | 2010 | 20405260 |
| the second anti-lipopolysaccharide factor (esalf-2) with antimicrobial activity from eriocheir sinensis. | the anti-lipopolysaccharide factor (alf) is a small basic protein that can bind and neutralize lipopolysaccharide (lps), mediating degranulation and activation of an intracellular coagulation cascade. in the present study, cdna of the second eriocheir sinensis alf (designated as esalf-2) was cloned and the full-length cdna of esalf-2 was of 724bp, consisting of an open reading frame (orf) of 363bp encoding a polypeptide of 120 amino acids. the deduced amino acid of esalf-2 shared 82% similarity ... | 2010 | 20416335 |
| differential regulation of sciaenops ocellatus viperin expression by intracellular and extracellular bacterial pathogens. | viperin is an antiviral protein that has been found to exist in diverse vertebrate organisms and is involved in innate immunity against the infection of a wide range of viruses. however, it is largely unclear as to the potential role played by viperin in bacterial infection. in this study, we identified the red drum sciaenops ocellatus viperin gene (sovip) and analyzed its expression in relation to bacterial challenge. the complete gene of sovip is 2570 bp in length and contains six exons and fi ... | 2010 | 20420911 |
| infection-induced changes in expression of antibacterial and cytokine genes in the gill epithelial cells of atlantic cod, gadus morhua during incubation with bacterial pathogens. | the molecular processes of immune responses in mucosal tissues, such as the gills, during infection with bacterial pathogens are poorly understood. in the present study, we analyzed the transcriptional profiles of selected antibacterial genes and cytokines in the gills of a cold-water fish, atlantic cod, gadus morhua following in vitro infection with bacterial pathogens, vibrio anguillarum and atypical aeromonas salmonicida using semi-quantitative rt-pcr. there was significant upregulation in th ... | 2010 | 20430108 |
| vaccination trials of sea bass (dicentrarchus labrax) against pasteurellosis using oral, intraperitoneal and immersion methods. | photobacterium damsela subsp. piscicida (phdp) is the aetiological agent of fish pasteurellosis, causing heavy losses in intensive mariculture plants. the present work compares the protective efficacy of five different vaccine formulation: oral, intraperitoneal, immersion, bivalent immersion (vibrio anguillarum) and immersion associated with immunostimulants. each of these vaccine formulations containing whole cells of phdp formalin inactivated (fkc), was administered to 100 sea bass weighing ap ... | 2005 | 20437375 |
| the first molluscan tctp in venerupis philippinarum: molecular cloning and expression analysis. | translationally controlled tumor protein (tctp) is one of the abundant and ubiquitously expressed proteins in metazoans. in the present study, the first molluscan tctp (denoted as vptctp) was identified from venerupis philippinarum haemocytes by est and race approaches. the full-length cdna of vptctp consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids. the deduced amino acid sequence of vptctp shared high similarity with tctps from other species, indicatin ... | 2010 | 20438851 |
| cloning and characterization of type ii interleukin-1 receptor cdna from japanese flounder (paralichthys olivaceus). | the type ii interleukin-1 receptor (il-1rii) cdna was cloned from japanese flounder (paralichthys olivaceus) by mrna differential display (dd-pcr) and rapid amplification of cdna ends (race). the full-length cdna is 1793 bp in length, including a 100 bp 5'-terminal untranslated region (utr), a 430 bp 3'-terminal utr, and a 1263 bp open reading frame (orf). the orf encodes 420 amino acid residues with an estimated molecular mass of 46.65 kda. the protein possesses signature features of the il-r f ... | 2010 | 20451651 |
| specificity of anti-vibrio immune response through p38 mapk and pkc activation in the hemocytes of the mussel mytilus galloprovincialis. | in mussel (mytilus sp.) hemocytes, differential functional responses to injection with different types of live and heat-killed vibrio species have been recently demonstrated. in this work, responses of mytilus hemocytes to heat-killed vibrio splendidus lgp32 and the mechanisms involved were investigated in vitro and the results were compared with those obtained with vibrio anguillarum (atcc 19264). adhesion of hemocytes after incubation with bacteria was evaluated by flow cytometry: both total h ... | 2010 | 20493193 |
| identification and characterization of a cystatin gene from chinese mitten crab eriocheir sinensis. | cystatins are a superfamily of proteins as reversible inhibitor of cysteine proteinases which play essential roles in a spectrum of physiological and immunological processes. in this study, a novel member of cystatin superfamily was identified from chinese mitten crab eriocheir sinensis (designated escystatin) by expressed sequence tag (est) analysis and rapid amplification of cdna ends (race) approaches. the full-length cdna of escystatin was of 1486 bp, consisting of a 5'-terminal untranslated ... | 2010 | 20570735 |
| mhc class iialpha gene polymorphism and its association with resistance/susceptibility to vibrio anguillarum in japanese flounder (paralichthys olivaceus). | association between polymorphism of the major histocompatibility complex (mhc) genes and disease resistance has been documented for few teleosts. in this study, we first investigate the genetic variation at the mhc iialpha gene in japanese flounder (paralichthys olivaceus) for survival after challenge with bacterial infection. to explore the specific allele associated with disease resistance, about 6000 individuals from 60 families challenged with vibrio anguillarum, which causes significantly d ... | 2010 | 20580738 |
| molecular cloning, characterization and expression analysis of cathepsin c gene involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of cathepsin c (catc) in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full-length catc cdna (1481 bp) was cloned via pcr based upon an initial expre ... | 2010 | 20600276 |
| cdna cloning and mrna expression of a selenium-dependent glutathione peroxidase from zhikong scallop chlamys farreri. | the glutathione peroxidases are essential enzymes of the cellular antioxidant defence system. in the present study, the full-length cdna sequence encoding an extracellular glutathione peroxidase (designated cfgpx3) was isolated from zhikong scallop chlamys farreri. the complete cdna was of 1194 bp, containing a 5' untranslated region (utr) of 50 bp, a 3' utr of 490 bp and an open reading frame (orf) of 654 bp encoding a polypeptide of 217 amino acids. cfgpx3 possessed all the conserved features ... | 2010 | 20601067 |
| effects of vibrio challenge on digestive gland biomarkers and antioxidant gene expression in mytilus galloprovincialis. | in bivalve molluscs, responses to bacterial infection have been largely characterized in terms of both functional responses and gene expression in the immune cells, the hemocytes. the effects of bacterial challenge at the tissue level, where bacterial infection may cause stressful conditions, have not been so far specifically investigated. biomarkers are widely utilised to evaluate the health status of bivalves, from the molecular to the organism level, in response to both natural and anthropoge ... | 2010 | 20601119 |
| molecular cloning, genomic structure and expression analysis of major histocompatibility complex class iα gene of half-smooth tongue sole (cynoglossus semilaevis). | major histocompatibility complex (mhc) has a central role in the adaptive immune system by presenting foreign peptide to the t-cell receptor. the full length of mhc class iα cdna was cloned from half-smooth tongue sole by homology cloning and rapid amplification of cdna ends polymerase chain reaction (race-pcr), genomic organization and expression of mhc iα were examined to study the function of mhc gene in fish. the domain structure feature and antigen-binding motifs of other teleost and mammal ... | 2011 | 20652829 |
| molecular cloning and expression of a relish gene in chinese mitten crab eriocheir sinensis. | nf-κb is a b-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. in the present study, a novel nf-κb-like transcription factor relish was cloned from chinese mitten crab eriocheir sinensis (designated as esrelish) by rapid amplification of cdna ends (race) technique based on expressed sequence tag (est). the full-length cdna of esrelish was of 5034 bp, consisting of a 5' untranslated region (utr) of 57 bp, a 3' utr of ... | 2010 | 20670337 |
| a variant of the interleukin-1beta gene in european sea bass, dicentrarchus labrax l., is associated with increased resistance against vibrio anguillarum. | vibriosis caused by the pathogenic bacterium vibrio (listonella) anguillarum leads to serious losses in european sea bass, dicentrarchus labrax. because of its pleiotropic activity in controlling immune and inflammatory responses against various pathogens, interleukin-1beta (il-1beta) is an attractive candidate for resistance to bacterial vibriosis. four polymorphisms c.76 + 52c>t, c.76 + 157a>g, c.76 + 215a>and c76 + 310a>g of il1b were genotyped in progeny of four families of wild sea bass cap ... | 2010 | 20690960 |
| molecular cloning, characterization, expression and activity analysis of cathepsin l in chinese mitten crab, eriocheir sinensis. | cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of cathepsin l (catl) in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full-length catl cdna (1274 bp) was cloned via pcr based upon an initial expre ... | 2010 | 20732429 |
| molecular cloning, characterization and expression analysis of cathepsin a gene in chinese mitten crab, eriocheir sinensis. | cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of cathepsin a (cata) in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full length cata cdna (2200 bp) was cloned via pcr based upon an initial expre ... | 2011 | 20817057 |
| prolactin-induced production of reactive oxygen species and il-1β in leukocytes from the bony fish gilthead seabream involves jak/stat and nf-κb signaling pathways. | prolactin (prl), a peptide hormone produced by the pituitary gland, was shown to play an important role in the modulation of the immune system of lower and higher vertebrates. to further investigate the effects of prl on the activation of professional phagocytes of bony fish, we stimulated head kidney leukocytes and purified macrophages from the gilthead seabream (sparus aurata l.) with various physiological concentrations of native salmon prl for 2 and 16 h and analyzed the respiratory burst ac ... | 2010 | 20826756 |
| screening of genes expressed in vivo after infection by vibrio anguillarum m3. | genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. this study aims to screen the genes expressed in vivo after vibrio anguillarum infection by in vivo-induced antigen technology (iviat). | 2010 | 20849396 |
| upregulation in response to infection and antibacterial activity of oyster histone h4. | several histones and histone-derived peptides have been shown to have antimicrobial activity and a potential role in innate immune defenses. a histone h4 sequence was identified in a subtractive suppression library containing genes upregulated in american cupped oysters, crassostrea virginica, in response to challenge with the protozoan parasite perkinsus marinus. oyster histone h4 protein levels significantly increased in hemocyte lysates and cell free hemolymph of oysters experimentally challe ... | 2010 | 20883794 |
| molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (alf) from the mud crab, scylla paramamosain. | antimicrobial peptides (amps) serve a major role in host defense systems against microbial invasion. in this study, a novel isoform (alfsp2) of antilipopolysaccharide factors (alfs) was cloned from the mud crab, scylla paramamosain. the open reading frame of the alfsp2 cdna is 348 bp and encodes for a predicted 115 amino acid residues (12.92 kda), and a mature protein of 94 amino acids and a molecular mass of 10.79 kda. the amino acid sequence of alfsp2 has an overall similarity of 74%, 66% and ... | 2010 | 20883796 |
| diet enriched with mushroom phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, epinephelus bruneus against vibriosis. | the effect of diet supplemented with phellinus linteus fed for 30 days was investigated in grouper epinephelus bruneus challenged with vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, and vibrio carchariae; infected and treated fish had a significantly higher percent weight gain and feed efficiency. in groups fed with enriched diet and challenged with v. anguillarum and v. harveyi the mortality rate declined with a consequent rise in survival rate than with other pathogens. on the other ... | 2010 | 20883799 |
| in vitro adherence of two candidate probiotics from atlantic cod and their interference with the adhesion of two pathogenic bacteria. | the potential of two candidate probiotic bacteria (gp21 and gp12), isolated from the gut of atlantic cod, to adhere to primary cultures of the epithelial cells from the different regions of the intestine and to interfere with the adhesion of two pathogens, vibrio anguillarum and aeromonas salmonicida subsp. salmonicida were investigated. the intestinal isolates showed clear preference in adhering to the cells from the different intestine segments. gp12 adhered strongly to the fore- and mid intes ... | 2010 | 20884135 |
| cloning and characterization of allograft inflammatory factor-1 (aif-1) from manila clam venerupis philippinarum. | allograft inflammatory factor-1 (aif-1) is a 17 kda interferon-γ-inducible ca(2+)-binding ef-hand protein that plays a significant role not only in different host responses to inflammatory stimuli, but in the whole host immune defense reaction. in the present study, the full-length cdna of aif-1 was identified from manila clam venerupis philippinarum (denoted as vpaif-1) by cdna library and race approaches. the cdna of vpaif-1 consisted of a 5-terminal untranslated region (utr) of 153 bp, a 3'ut ... | 2010 | 20933601 |
| molecular characterization of a mollusk chicken-type lysozyme gene from haliotis discus hannai ino, and the antimicrobial activity of its recombinant protein. | a chicken-type lysozyme was obtained and characterized from the abalone haliotis discus hannai ino (hdlysc). the full-length cdna of hdlysc was 586 bp, and it contained an open reading frame of 441 bp, encoding a 147-amino acid protein with a calculated molecular mass of 15.64 kda and an isoelectric point of 4.87. the amino acid sequence of hdlysc possessed all conserved features critical for the fundamental structure and function of c-type lysozymes, including the two catalytic residues, glu54 ... | 2010 | 20951809 |
| molecular characterization of a novel big defensin from clam venerupis philippinarum. | antimicrobial peptides (amps) are important mediators of the primary defense mechanism against microbial invasion. in the present study, a big defensin was identified from venerupis philippinarum haemocytes (denoted as vpbd) by race and est approaches. the vpbd cdna contained an open reading frame (orf) of 285 bp encoding a polypeptide of 94 amino acids. the deduce amino acid sequence of vpbd shared the common features of big defensin including disulfide array organization and helix structure, i ... | 2010 | 20975988 |
| detection of autoinducer (ai-2)-like activity in food samples. | the contamination, survival, and possible foodborne disease outbreaks are major issues confronting the food industry. however, from a microbial perspective, any food whether natural or processed is just another environmental niche that is available for colonization. quorum sensing or cell-cell communication is a process by which microorganisms are thought to communicate with each other using a variety of small molecules termed autoinducers. the autoinducer ai-2 is thought to be a universal signa ... | 2011 | 21031305 |
| signal transducer and activator of transcription 3 (stat3) homologue in turbot (scophthalmus maximus): molecular characterization and expression analysis. | signal transducer and activator of transcription 3 (stat3) acts as an important mediator in multiple biological processes induced by different cytokines. so far, little information is available in fish stat3. in this study, turbot (scophthalmus maximus) stat3 gene was cloned and characterized for the first time. the turbot stat3 full-length cdna consists of 2355 nucleotides encoding a polypeptide of 784 amino acids with four conserved domains including stat_int, stat_alpha, stat_bind and sh2 dom ... | 2010 | 21035551 |
| molecular cloning, characterization and expression analysis of two apoptosis genes, caspase and nm23, involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | apoptosis is a central regulatory feature of the immune system, and the most common form of death among immunological cells. however, the function of apoptosis, within the innate immune system of invertebrates, remains largely unknown. for this reason, we investigated the immune functionality of two apoptosis genes, caspase and nm23, in the chinese mitten crab (eriocheir sinensis), which is a commercially important and disease vulnerable aquaculture species. the entire length caspase and nm23 cd ... | 2010 | 21050890 |
| alternation of venerupis philippinarum hsp40 gene expression in response to pathogen challenge and heavy metal exposure. | hsp40 was an understudied protein family with co-chaperone activity. in the present study, a hsp40 homology was cloned from venerupis philippinarum haemocytes (designated as vphsp40) by est analysis and race approaches. the expression profiles of vphsp40 under vibrio anguillarum challenge and heavy metal exposure were investigated by quantitative real-time rt-pcr. the bacterial challenge could significantly up-regulate the mrna expression, and the highest expression level was detected at 24 h po ... | 2010 | 21056105 |
| an anti-lipopolysaccharide factor from red swamp crayfish, procambarus clarkii, exhibited antimicrobial activities in vitro and in vivo. | the anti-lipopolysaccharide factors (alfs) are a group of effector molecules of innate immunity in arthropods, exhibiting binding and neutralizing activities to lipopolysaccharides. in this study, an alf cdna sequence (pcalf1) was identified from red swamp crayfish, procambarus clarkii. the deduced peptide of pcalf1 was conserved; it manifested the signal peptide and lipopolysaccharide (lps)-binding domain, especially the two conserved cysteine residues at both ends of the domain. transcripts of ... | 2010 | 21056107 |
| mutation of a novel virulence-related gene mltd in vibrio anguillarum enhances lethality in zebra fish. | vibrio anguillarum, a halophilic gram-negative bacterium, is the causative agent of vibriosis, which is a major problem for the aquaculture industry worldwide. previously, a virulence-related gene fragment of v. anguillarum was obtained from a suppression subtractive hybridization (ssh) library. in this study, the complete gene sequence was obtained by long and accurate pcr (la-pcr). after sequence analysis and homologous comparison, this new virulence-related gene was revealed to encode a putat ... | 2010 | 21070855 |
| vibriosis vaccines based on various sero-subgroups of vibrio anguillarum o2 induce specific protection in atlantic cod (gadus morhua l.) juveniles. | the purpose of this study was to investigate the efficacy of three monovalent and a trivalent vibriosis dip vaccines in juvenile atlantic cod (gadus morhua l.), examine whether the responses were specific and study the expression of selected immune genes after dip vaccination. in addition, the study addressed whether the deviating isolates of vibrio anguillarum serotype o2 belongs to another sero-subgroup than the previously established sero-subgroups o2a, o2b and o2c. rabbit v. anguillarum sero ... | 2010 | 21078394 |
| cloning, genomic structure, and expression analysis of peroxiredoxin v from bay scallop argopecten irradians. | peroxiredoxins (prxs) constitute a superfamily of antioxidative proteins that play important roles in protecting organisms against damage from reactive oxygen species (ros). in this study, the peroxiredoxin v gene from argopecten irradians (ai-prxv) was isolated and characterized. the full-length ai-prxv cdna consists of 1689 bp with a 567 bp open reading frame (orf) that encodes 188 amino acids. three putative polyadenylation consensus signals (aataaa) were found in the 953 bp long 3'-utr. the ... | 2010 | 21078396 |
| subcellular components of probiotics kocuria sm1 and rhodococcus sm2 induce protective immunity in rainbow trout (oncorhynchus mykiss, walbaum) against vibrio anguillarum. | the efficacy of cellular components of probiotics kocuria sm1 and rhodococcus sm2 to protect rainbow trout (oncorhynchus mykiss, walbaum) against vibriosis was assessed. groups of fish (average weight = 10-15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e., 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ecps), cell wall proteins (cwps) and whole cell proteins (wcps) of sm1 and sm2, respectively, or with 0.1 ml of phosphate-buffered saline ... | 2010 | 21078398 |
| molecular cloning, characterization and expression analysis of macrophage migration inhibitory protein (mif) in chinese mitten crab, eriocheir sinensis. | macrophage migration inhibitory factor (mif) as a multi-functional cytokine mediating both innate and adaptive immune responses, however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of mif in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full-length mif cdna (704 bp) was cloned via pcr based upon an initial expressed sequence tag (e ... | 2010 | 21087669 |
| vancomycin-functionalised ag@tio2 phototoxicity for bacteria. | this study reports on the synthesis of vancomycin (van)-functionalised ag@tio(2) nanoparticles and their enhanced bactericidal activities. van-ag@tio(2) nanoparticles were prepared by nanoparticle deposition and chemical cross-linking reactions. the catalysts showed high efficiency for the degradation of methylene blue under ultraviolet (uv) illumination. the photocatalytic inactivation of the sulphate-reducing bacteria, desulfotomaculum, was also studied under uv light irradiation and in the da ... | 2010 | 21115223 |
| cdna cloning and characterization of a new member of the tumor necrosis factor receptor family gene from scallop, chlamys farreri. | tumor necrosis factors receptor (tnfr) is a superfamily of proteins derived mainly from vertebrates. it plays significant role in diverse physiological and pathological events such as inflammation, apoptosis, autoimmunity and organogenesis. the gene of a new member of tnfr family, designated as cftnfr2, was cloned and characterized from scallop, chlamys farreri. the full-length cdna of cftnfr2 consisted of 1,501 nucleotides with a poly (a) tail, encoding a polypeptide of 378 amino acids with the ... | 2010 | 21132383 |
| comparison of 16srdna and toxr genes as targets for detection of vibrio anguillarum in dicentrarchus labrax kidney and liver. | vibrio anguillarum is a pathogen that causes high mortality in marine and freshwater fish. the aim of this study was to develop a real-time pcr assay for identification and quantification of v. anguillarum in fish tissue. the assay was carried out using two target genes, 16srdna and toxr, to evaluate the influence of differences in the operon copy number in quantitative assessment, both in pure cultures of v. anguillarum serovar o1 (strain 975/i), as a reference, and in the liver and kidney of a ... | 2010 | 21145392 |
| genomic sequences comparison and differential expression of miiuy croaker mhc class i gene, after infection by vibrio anguillarum. | major histocompatibility complex (mhc) has a central role in the adaptive immune system by presenting foreign peptide to the t-cell receptor. mhc gene family contains two main subgroups of immunologically active molecules. in order to study the molecular function and genomic characteristic of class i gene in teleost, the full lengths of mhc class iα cdna and genomic sequence were cloned from miiuy croaker (miichthys miiuy). seven exons and six introns were identified in miiuy croaker class iα ge ... | 2010 | 21147159 |
| development of a single base extension-tag microarray for the detection of pathogenic vibrio species in seafood. | in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ... | 2010 | 21161212 |
| a primitive toll-like receptor signaling pathway in mollusk zhikong scallop chlamys farreri. | as a member of pattern-recognition receptors (prrs), the toll-like receptor (tlr) and its signaling pathway play pivotal roles in recognizing various pathogen-associated molecular patterns (pamps), and buildup the front-line against invading pathogens. in the present study, the sequence features and mrna expression profiles of five key genes involved in tlr signal pathway were characterized, and their functions in the immune responses were also investigated in order to validate the tlr signaling ... | 2010 | 21167199 |
| [molecular cloning, sequence analysis and expression pattern of hepcidin gene in ayu (plecoglossus altivelis)]. | hepcidin, a member of cysteine-rich antimicrobial peptides, plays an important role in both fish adaptive immunity and the regulation of iron metabolism. in this paper, the nucleotide sequence of a full-length cdna clone for ayu (plecoglossus altivelis) hepcidin gene, 763 nucleotides in length, was determined. ayu hepcidin gene contained a complete open reading frame (orf) encoding an 85-amino-acid peptide with a molecular weight of 9.7 k. a signal peptide of 24 residues existed in hepcidin n-te ... | 2010 | 21174348 |
| the effects of pollution on fish health. | potentially harmful substances-e.g. pesticides, heavy metals and hydrocarbons-are often released into the aquatic environment. when large quantities of pollutants are released there may be an immediate impact as measured by large-scale sudden mortalities of aquatic organisms, e.g. fish kills resulting from contamination of waterways with agricultural pesticides. lower levels of discharge may result in an accumulation of the pollutants in aquatic organisms. the end results, which may occur long a ... | 1998 | 21182713 |
| bacteriocin activity and probiotic activity of aeromonas media. | three strains of aeromonas media (161, a164 and a199) were shown to be active in-vitro producers of bacteriocin-like inhibitory substances (blis). for example, the producer strain, aer. media a199, displayed antagonistic activity against all strains tested of aer. caviae, aer. hydrophila, aer. salmonicida, aer. veronii var. sobria, listonella anguillarum, photobacterium damsella, eight species of vibrio and yersinia ruckeri. because of this wide-ranging activity against fish/shellfish pathogens, ... | 1998 | 21182714 |
| infection and immunization trials of asian seabass (lates calcarifer) against fish pathogen vibrio anguillarum. | vibriosis is one of the most prevalent fish diseases caused by bacteria belonging to the genus vibrio sp. fish disease will be controlled by proper vaccination trials and maintenance of fish form. pathogenicity for asian seabass (lates calcarifer) against v anguillarum results in necrosis and haemorrhagic areas near the base of fins, exopthalmia and ulcers on the skin surface. around 50, 100, 200 microl of formalin killed bacterial cells were injected (intraperitoneal) to three different size (5 ... | 2010 | 21186732 |
| influence of alginic acid and fucoidan on the immune responses of head kidney leukocytes in cod. | the present study investigated the immunomodulatory activities of alginic acid and fucoidan, both derived from brown seaweeds, on selected cellular immune responses and antibacterial activity of head kidney (hk) leukocytes of cod, gadus morhua. primary cultures of hk leukocytes were incubated with either 10 or 100 μg ml(-1) of the substances and the effects on respiratory burst, cellular proliferation, acid and alkaline phosphatase activity and cellular myeloperoxidase were measured at 3- and 24 ... | 2010 | 21191809 |
| a crassostrea gigas toll-like receptor and comparative analysis of tlr pathway in invertebrates. | toll-like receptor (tlr) signaling pathway was an important and evolutionarily conserved innate immune pathway. evolutionary lineage of this pathway in the lophotrochozoans is still less understood. in this study, we cloned a novel tlr, a key component of tlr pathway, from crassostrea gigas, and named it cgtoll-1. the 4343 base pairs full-length cdna was assembled with the 3' and 5' race (rapid amplification of cdna ends) pcr results, and containing a 3540 bp open reading frame, which encoding a ... | 2010 | 21195773 |
| vibrio vulnificus rtxa1 gene recombination generates toxin variants with altered potency during intestinal infection. | vibrio vulnificus is a food-borne bacterial pathogen associated with 1% of all food-related deaths, predominantly because of consumption of contaminated seafood. the ability of v. vulnificus to cause disease is linked to the production of a large cytotoxin called the "multifunctional-autoprocessing rtx" (martx(vv)) toxin, a factor shown here to be an important virulence factor by the intragastric route of infection in mice. in this study, we examined genetic variation of the rtxa1 gene that enco ... | 2011 | 21220343 |
| a galectin with quadruple-domain from bay scallop argopecten irradians is involved in innate immune response. | galectins are a family of β-galactoside-binding lectins that specifically bind to β-galactoside residues and play crucial roles in innate immune responses of invertebrates and vertebrates. the cdna of bay scallop argopecten irradians galectin (designated as aigal2) was cloned by rapid amplification of cdna ends (race) method based on the expressed sequence tag (est). the full-length cdna of aigal2 was of 2137 bp. the open reading frame encoded a polypeptide of 555 amino acids containing four car ... | 2011 | 21237194 |
| gene discovery, comparative analysis and expression profile reveal the complexity of the crassostrea gigas apoptosis system. | apoptosis system was reported to play important role in organism immunity, but it was a currently understudied respect in molluscan immunity studies. base on the recent generation of ests in the pacific oyster, crassostrea gigas, a survey of apoptosis-related molecules was conducted in the assembled unigenes, we found that the basic genes and domains in apoptosis-associated proteins were conserved, the overall apoptotic machinery was complex in c. gigas and that the organism had an expanded numb ... | 2011 | 21237195 |
| surface display of aeromonas hydrophila gapdh in attenuated vibrio anguillarum to develop a noval multivalent vector vaccine. | displaying foreign antigens on the surface of attenuated or avirulent bacteria is an important strategy to develop live multivalent vector vaccines. in our previous work, several efficient surface display systems have been established based on outer membrane anchoring elements, which could successfully display heterologous proteins in attenuated vibrio anguillarum. in this work, the glyceraldehyde-3-phosphate dehydrogenase (gapdh) from pathogenic aeromonas hydrophila lsa34 was fused to seven dis ... | 2011 | 21246232 |
| molecular cloning and expression analysis of signal transducer and activator of transcription (stat) from the chinese white shrimp fenneropenaeus chinensis. | innate immunity is the first line of defense by a host against invading pathogens. several signaling pathways participate in the immune response, one of which is the janus kinase (jak)/signal transducer and activator of transcription (stat) pathway. various evidences have been provided to suggest that the jak/stat pathway is involved in both antibacterial and antiviral immunities. in this study, the full-length cdna and gene sequence of stat (designated as fcstat) was cloned from the chinese whi ... | 2011 | 21246285 |
| structure-activity relationship of cinnamaldehyde analogs as inhibitors of ai-2 based quorum sensing and their effect on virulence of vibrio spp. | many bacteria, including vibrio spp., regulate virulence gene expression in a cell-density dependent way through a communication process termed quorum sensing (qs). hence, interfering with qs could be a valuable novel antipathogenic strategy. cinnamaldehyde has previously been shown to inhibit qs-regulated virulence by decreasing the dna-binding ability of the qs response regulator luxr. however, little is known about the structure-activity relationship of cinnamaldehyde analogs. | 2011 | 21249192 |
| a novel c-type lectin from bay scallop argopecten irradians (aictl-7) agglutinating fungi with mannose specificity. | c-type lectins are a superfamily of proteins that can bind pathogen-associated molecular patterns (pamps) and microorganisms through the recognition of carbohydrates, thus they are directly involved in innate defense mechanisms as part of the acute-phase response to infection. in this study, the cdna of a novel c-type lectin (designated as aictl-7) was cloned from bay scallop argopecten irradians by expression sequence tag (est) analysis and rapid amplification of cdna ends (race) approach. the ... | 2011 | 21255651 |
| genetic and biochemical analyses of chromosome and plasmid gene homologues encoding icl and arcp domains in vibrio anguillarum strain 775. | anguibactin, the siderophore produced by vibrio anguillarum 775 is synthesized from 2,3-dihydroxybenzoic acid (dhba), cysteine and hydroxyhistamine via a nonribosomal peptide synthetase (nrps) mechanism. most of the genes encoding anguibactin biosynthetic proteins are harbored by the pjm1 plasmid. in this work we report the identification of a homologue of the plasmid-encoded angb on the chromosome of strain 775. the product of both genes harbor an isochorismate lyase (icl) domain that converts ... | 2011 | 21286786 |
| the genus listonella macdonell and colwell 1986 is a later heterotypic synonym of the genus vibrio pacini 1854 (approved lists 1980) - a taxonomic opinion. | we analyzed the taxonomic position of the genus listonella based on phylogenetic, genomic and phenotypic data. the species of the genus listonella were nested within the genus vibrio according to the 16s rrna-based phylogenetic tree. the closest neighbor of vibrio listonella) anguillarum (lmg4437t and atcc 68554=775) was vibrio ordalii (lmg 13544t) with more than 99.5 % gene sequence similarity. furthermore, v. (listonella) pelagius is highly related to vibrio splendidus. according to average am ... | 2011 | 21296930 |
| role of the pjm1 plasmid-encoded transport proteins fatb, c and d in ferric anguibactin uptake in the fish pathogen vibrio anguillarum. | vibrio anguillarum serotype o1 is part of the natural flora in the aquatic habitat, but under certain circumstances it can cause terminal haemorrhagic septicemia in marine and fresh water fish due to the action of the anguibactin iron uptake system encoded by the virulence plasmid pjm1. this plasmid harbours the genes for the biosynthesis of the siderophore anguibactin and the ferric anguibactin transport proteins fatd, c, b and a encoded in the iron transport operon. the fata protein is the out ... | 2010 | 21304833 |
| bioactivity, chemical profiling, and 16s rrna-based phylogeny of pseudoalteromonas strains collected on a global research cruise. | one hundred one antibacterial pseudoalteromonas strains that inhibited growth of a vibrio anguillarum test strain were collected on a global research cruise (galathea 3), and 51 of the strains repeatedly demonstrated antibacterial activity. here, we profile secondary metabolites of these strains to determine if particular compounds serve as strain or species markers and to determine if the secondary metabolite profile of one strain represents the bioactivity of the entire species. 16s rrna gene ... | 2011 | 21305330 |
| taxonomy of bacterial fish pathogens. | abstract: bacterial taxonomy has progressed from reliance on highly artificial culture-dependent techniques involving the study of phenotype (including morphological, biochemical and physiological data) to the modern applications of molecular biology, most recently 16s rrna gene sequencing, which gives an insight into evolutionary pathways (= phylogenetics). the latter is applicable to culture-independent approaches, and has led directly to the recognition of new uncultured bacterial groups, i.e ... | 2011 | 21314902 |
| c-type lectin from red swamp crayfish procambarus clarkii participates in cellular immune response. | lectins are potential immune recognition proteins. in this study, a novel c-type lectin (pc-lec1) is reported in freshwater crayfish procambarus clarkii. pc-lec1 encodes a protein of 163 amino acids with a putative signal peptide and a single carbohydrate recognition domain. it was constitutively expressed in various tissues of a normal crayfish, especially in the hepatopancreas and gills. expressions of pc-lec1 were up-regulated in the hepatopancreas and gills of crayfish challenged with vibrio ... | 2011 | 21322006 |
| evidence for the role of horizontal transfer in generating pvt1, a large mosaic conjugative plasmid from the clam pathogen, vibrio tapetis. | the marine bacterium vibrio tapetis is the causative agent of the brown ring disease, which affects the clam ruditapes philippinarum and causes heavy economic losses in north of europe and in eastern asia. further characterization of v. tapetis isolates showed that all the investigated strains harbored at least one large plasmid. we determined the sequence of the 82,266 bp plasmid pvt1 from the cect4600(t) reference strain and analyzed its genetic content. pvt1 is a mosaic plasmid closely relate ... | 2011 | 21326607 |
| effects of three heavy metals on the bacteria growth kinetics: a bivariate model for toxicological assessment. | the effects of three heavy metals (co, ni and cd) on the growth kinetics of five bacterial strains with different characteristics (pseudomonas sp., phaeobacter sp. strain 27-4, listonella anguillarum, carnobacterium piscicola and leuconostoc mesenteroides subsp. lysis) were studied in a batch system. a bivariate model, function of time and dose, is proposed to describe simultaneously all the kinetic profiles obtained by incubating a microorganism at increasing concentrations of individual metals ... | 2011 | 21360150 |
| cloning and characterization of a sialic acid binding lectins (sabl) from manila clam venerupis philippinarum. | sialic acid binding lectin (sabl) is a member of immunoglobulin-like lectins family that are thought to promote cell-cell interactions and regulate the functions of cells in the innate and adaptive immune systems through glycan recognition. in the present study, the full-length cdna of sabl was identified from manila clam venerupis philippinarum (denoted as vpsabl) by cdna library and race approaches. the cdna of vpsabl consisted of a 5'terminal untranslated region (utr) of 62 bp, a 3' utr of 35 ... | 2011 | 21376125 |
| molecular characterization of miiuy croaker cc chemokine gene and its expression following vibrio anguillarum injection. | a cc chemokine gene was isolated from miiuy croaker (miichthys miiuy) by expressed sequence tag analysis. the mimi-cc cdna contains an open reading frame of 429 nucleotides encoding 142 amino acid residues. the deduced mimi-cc possesses the typical arrangement of four cysteines as found in other known cc chemokines (c³¹, c³², c56, and c7°). it shares 15.3%-37.4% identity to cc chemokines of mammal and teleost. phylogenetic analysis showed that miiuy croaker was most closely related to atlantic c ... | 2011 | 21414411 |
| a monoamine oxidase from scallop chlamys farreri serving as an immunomodulator in response against bacterial challenge. | monoamine oxidase (mao) is an essential enzyme in the catabolism of monoamines, and implicated in the immune response of vertebrates. in the present study, the full-length cdna encoding monoamine oxidase (designated cfmao) was cloned from chlamys farreri by using rapid amplification of cdna ends (race) approaches and expression sequence tag (est) analysis. the open reading frame of cfmao cdna encoded 519 amino acids, which shared 73.9% similarity with that from oyster crassostrea gigas, and 64.5 ... | 2011 | 21420424 |
| microbial ecology of fish species ongrowing in greek sea farms and their watery environment. | the present study focuses on the bacteriological profile of both watery ecosystem and fishes from different north and central west greek fish-farms ongrowing euryhaline fish species. the natural microflora of the fish and the water of their ongrowing units in selected farms were studied for a period of 20 months. the analyzed samples were mainly sea bream (sparus aurata) 61.3% and sea bass (dicentrarchus labrax) 24%. in most of the watery ecosystems coming from the different sampling areas, tota ... | 2011 | 21421068 |
| an interleukin-2 enhancer binding factor 2 homolog involved in immune response from chinese mitten crab eriocheir sinensis. | as a transcription factor, interleukin-2 enhancer binding factor 2 (ilf2) regulates il-2 gene at level of transcription, splicing and translation in vertebrates and plays significant roles in immune system. in this study, an ilf2 homolog was identified from chinese mitten crab eriocheir sinensis (designated as esilf) by expressed sequence tag (est) analysis. the full-length cdna of esilf was of 2159bp, containing a 5' untranslated region (utr) of 90bp, a 3' utr of 866bp with a poly (a) tail, and ... | 2011 | 21439385 |
| iron(iii) complexation by vanchrobactin, a siderophore of the bacterial fish pathogen vibrio anguillarum. | the bacterial fish pathogen vibrio anguillarum serotype o2 strain rv22 produces the mono catecholate siderophore vanchrobactin (vb) under conditions of iron deficiency. vb contains two potential bidentate coordination sites: catecholate and salicylate groups. the iron(iii) coordination properties of vb is investigated in aqueous solutions using spectrophotometric and potentiometric methods. the stepwise equilibrium constants (log k) for successive addition of vb dianion to a ferric ion are 19.9; ... | 2011 | 21494744 |
| molecular and functional characterization of the gilthead seabream β-defensin demonstrate its chemotactic and antimicrobial activity. | antimicrobial peptides (amps) are important mediators of the innate immune response against bacteria and viruses. we have found a β-defensin (bd) gene searching the expressed sequence tags (ests) of the teleost fish gilthead seabream (sparus aurata). the clone contains an open reading frame of 201bp mrna that encodes a putative seabream β-defensin (sabd) propeptide of 66 amino acids containing the six conserved cysteines as the main signature of this amp. the phylogenetic tree shows that sabd, a ... | 2011 | 21497909 |
| a novel type iii crustin (cruses2) identified from chinese mitten crab eriocheir sinensis. | antimicrobial peptides are important effectors in the host innate immune response against microbial invasion. in the present study, the cdna encoding a crustin (designated cruses2) was cloned from chinese mitten crab eriocheir sinensis by using est analysis and rapid amplification of cdna ends (race) approach. the full-length cdna of cruses2 was of 1237 bp, containing a 5' untranslated region (utr) of 12 bp, a 3' utr of 886 bp with a poly (a) tail, and an open reading frame (orf) of 339 bp encod ... | 2011 | 21549196 |
| identification and expression of a putative lps-induced tnf-α factor from asiatic hard clam meretrix meretrix. | lps-induced tnf-α (litaf) is a novel transcriptional factor that mediates the expression of inflammatory cytokines in lps-induced processes. in the present study, the full-length cdna encoding litaf (designated as mm-litaf) was identified from asiatic hard clam, meretrix meretrix, by expressed sequence tag and rapid amplification of cdna ends (race) approaches. the full-length cdna of mm-litaf was 1653 bp, consisting of a 5' untranslated region (utr) of 91 bp, a 3'utr of 1166 bp with one cytokin ... | 2011 | 21567197 |
| complete genome sequence of the marine fish pathogen vibrio anguillarum harboring the pjm1 virulence plasmid and genomic comparison with other virulent strains of v. anguillarum and v. ordalii. | we dissected the complete genome sequence of the o1 serotype v. anguillarum 775 (pjm1), and determined the draft genomic sequences of the plasmidless strains of serotype o1, 96f and o2β, rv22 and v. ordalii. all strains harbor two chromosomes but 775 also carries the virulence plasmid pjm1 that encodes the genes to produce anguibactin and cognate transport genes, one of the main virulence factors of v. anguillarum. genomic analysis identified eight genomic islands in chromosome 1 of v. anguillar ... | 2011 | 21576332 |
| genetic determinants of virulence in the marine fish pathogen vibrio anguillarum. | one of the most studied fish pathogens is vibrio anguillarum. development of the genetics and biochemistry of the mechanisms of virulence in this fish pathogen together with clinical and ecologic studies has permitted the intensive development of microbiology in fish diseases. it is the intention of this review to compile the exhaustive knowledge accumulated on this bacterium and its interaction with the host fish by reporting a complete analysis of the v. anguillarum virulence factors and the g ... | 2011 | 21625345 |
| molecular characterization and expression analysis of an inhibitor of nf-+¦b (i+¦b) from asiatic hard clam meretrix meretrix. | inhibitor of nf-+¦b (i+¦b) is an important member of rel/nf-+¦b signaling pathway, which is an important mediator of immune responses in innate immune system. in this study, the i+¦b cdna of hard clam meretrix meretrix (designated as mm-i+¦b) was cloned and characterized. the full-length cdna of mm-i+¦b was of 2098 bp, containing a 5' untranslated region (utr) of 123 bp, a 3' utr of 810 bp with a poly (a) tail, and an open reading frame (orf) of 1164 bp encoding a polypeptide of 387 amino acids. ... | 2011 | 21689757 |