Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| an improved analytical method, based on hplc with electrochemical detection, for monitoring exposure to 3-chloro-4-fluoroaniline. | an hplc method for monitoring exposure of workers to 3-chloro-4-fluoroaniline (cfa) is described. it is based on the detection of a major urinary metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, and is superior to the previously adopted gc method. the limit of detection for the metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, is less than or equal to 0.01 mg/l (as cfa equivalent), with a coefficient of variation obtained over the range 1-15 mg/l of 4%. the hplc method avoids the need f ... | 1988 | 3244272 |
| detection of arthritogenic factor in adjuvant arthritis. | an evocation of arthritis by an ag-specific lymphokine has recently been considered with the description of arthritogenic factor (af) in rats with collagen arthritis. because rats with cfa-induced arthritis also exhibit t cell reactivity to native type ii collagen, t cell lines specific for this protein were established from cfa-injected rats. supernatant material from these lines contained a type ii collagen-binding lymphokine with functional and biochemical attributes identical with those desc ... | 1988 | 3257989 |
| immunologic enhancement of b16 melanoma growth. | in certain experimental tumor models, tumor growth is less pronounced in immune deficient animals. characteristically, tumors such as the murine b16 melanoma and lewis lung carcinoma (3ll) are weakly antigenic. we proposed that with such tumors that are weakly antigenic, growth is enhanced by t-cell factors. young mice were inoculated with irradiated b16 cells in complete freunds adjuvant (cfa) on three occasions, each separated by 2 weeks. specific antibody (igg) to b16 membrane antigens was de ... | 2011 | 3258540 |
| the role of immunity to cartilage proteoglycan in adjuvant arthritis. intravenous injection of bovine proteoglycan enhances adjuvant arthritis. | it has been suggested that autoimmunity to the proteoglycan (pg) component of cartilage plays a major role in the etiology of adjuvant arthritis (aa), which occurs in rats, but not in mice, after injection of cfa. in order to more directly investigate this role, bovine and human cartilage pg were used to modulate aa, and immunity to pg was assessed. immunization of rats or mice with pg by itself does not induce arthritis. however, in rats, a single i.v. injection of soluble pg, given 1 wk before ... | 1988 | 3260920 |
| induction of anterior chamber associated immune deviation in rats receiving intracameral injections of retinal s antigen. | the injection of a variety of antigens, including transplantation antigens, haptens, and viral glycoproteins, into the anterior chamber of eyes of mice produces a characteristic spectrum of immune responses in which suppressed cell mediated immunity dominates. this phenomenon is called anterior chamber associated immune deviation (acaid). having recently demonstrated that soluble antigens also can induce acaid in mice, we examined the possibility that an important soluble ocular antigen, retinal ... | 1988 | 3261228 |
| monitoring of interleukin-2 receptor (il-2r) expression in vivo and studies on an il-2r-directed immunosuppressive therapy of active and adoptive adjuvant-induced arthritis in rats. | recent evidence indicates that adjuvant arthritis (aa) of rats induced by complete freund's adjuvant (cfa) is an autoimmune disease that is mediated by t cells. this report describes the distribution of activated il-2 receptor (il-2r)-bearing cells in spleen, popliteal lymph nodes (pln) and blood in aa rats and in naive healthy rats using the monoclonal antibody (mab) art-18. it was found that in the primary lymph nodes (injected side) two peaks of elevated numbers of il-2r-positive cells (day 9 ... | 1988 | 3262576 |
| demyelination of sciatic nerve in acute experimental allergic neuritis of the rat. a gel electrophoresis investigation. | acute experimental allergic neuritis (ean) of lewis rats was monitored by clinical and electrophysiological tests for 60 days. sciatic nerve myelin proteins were analyzed by quantitative microgel electrophoresis and electroimmunoblotting at different clinical stages of the disease. the clinical severity of ean and the demyelination as measured by electrophysiological tests and myelin protein concentrations in sciatic nerve did not correspond during the course of the disease. demyelination reache ... | 1988 | 3263390 |
| disease patterns in experimental allergic neuritis (ean) in the lewis rat. is ean a good model for the guillain-barré syndrome? | lewis rats develop experimental allergic neuritis (ean) when injected with bovine dorsal root (bdr) emulsified in complete freund's adjuvant (cfa). in this study the susceptibility to ean and subsequent relapse was studied in animals ranging from 4 to 25 weeks of age. older animals exhibited a severe acute illness which was monophasic over the period of observation, whereas younger animals developed a less severe and frequently relapsing illness. very young animals often relapsed more than once ... | 1988 | 3265717 |
| a prospective study of serum antibody responses to enterotoxinogenic escherichia coli in swedish travellers. | serological responses to relevant enterotoxinogenic escherichia coli (etec) antigens were studied in 85 swedish travellers to subtropical and tropical areas. serum samples were collected from the travellers before and then after 3-5 weeks, i.e. within a week after return to sweden, when a faecal specimen was also taken. 40% of the participants had traveller's diarrhoea during their visit abroad, while 21% reported "loose stools" and 39% had no such problems. etec was rarely isolated from the sto ... | 1988 | 3283923 |
| immunodiabetes in rabbits. | the changes of basal glycemia and of that during the glucose and insulin tolerance tests as well as of the production of hemagglutinating antibodies in rabbits immunized with crystalline insulin (ci), chromatographically purified insulin (cpi) and waste products of the chromatographic purification of insulin (wp), both with and without complete freund's adjuvant (cfa) were studied. following immunization with ci and its components the glycemia increased characteristically and the course of gluco ... | 1988 | 3286223 |
| active immunization of heifers against luteinizing hormone-releasing hormone, human chorionic gonadotropin and bovine luteinizing hormone. | seventy crossbred heifers were allotted randomly to 10 treatment groups. treatments consisted of active immunization against ovalbumin (ov) conjugates of luteinizing hormone-releasing hormone (lhrh), human chorionic gonadotropin (hcg) and bovine luteinizing hormone (blh) with each of three adjuvants. the adjuvants were complete freund's adjuvant (cfa), m103(6) and 6vr6. control animals were immunized against ov alone using cfa. bulls were placed with the heifers following immunization to allow c ... | 1988 | 3288600 |
| characterization of tick antigens inducing host immune resistance. ii. description of rabbit-acquired immunity to amblyomma americanum ticks and identification of potential tick antigens by western blot analysis. | feeding by adult amblyomma americanum ticks induced a level of immunity in rabbits to subsequent tick feeding that resulted in a significant decrease in tick feeding success and fecundity. histological analysis of tick feeding sites in hosts expressing resistance revealed a predominant eosinophil response, with weak basophil and neutrophil infiltrates. while the basophil was never the dominant granulocyte at the tick feeding sites in resistant hosts, this cell exhibited the greatest increase in ... | 1988 | 3291384 |
| colonization, diarrhoea and protective immunogenicity of a cfa-deficient, enterotoxin-producing escherichia coli mutant in a non-ligated intestine experimental model. | a colonization factor antigen (cfa)-deficient mutant has been isolated that is as efficient as the cfa/i-carrying, enterotoxin-producing e. coli strain it was derived from in producing diarrhoea and colonizing the intestine in a rabbit non-ligated intestine model, the ritard model. infection with 10(11) mutant bacteria induced diarrhoea in 15 of the 17 rabbits challenged; corresponding diarrhoeal attack rates after challenge with similar doses of the original cfa/i-positive strain and of a non-e ... | 1987 | 3296650 |
| comparison of cfa/i:st plasmids of enterotoxigenic escherichia coli. | 1987 | 3298460 | |
| small-bowel colonization alone is a cause of diarrhea. | bacterial overgrowth in the small bowel is known to occur in patients with persistent diarrhea, but it has been unclear if colonization alone causes fluid secretion. we studied isogenic, nontoxigenic escherichia coli strains with colonizing factor antigen ii (cfa/ii) (1392+) and without cfa/ii (1392-) in 18-h rabbit ileal loops. neither strain produced fluid, but quantitative cultures of rabbit ileal loops with the cfa/ii-positive strain had a mean of 10(3) more cfu of bacteria per cm2 than did ... | 1987 | 3301680 |
| potentiation of autoimmune response in rats infected with toxoplasma gondii. | the influence that an infection with toxoplasma gondii in thymus proximity produces on the autoimmune response was studied. rats infected with 3 x 10(3) trophozoites of t. gondii in thymus proximity were immunized with 5 mg/ml of chemically modified rat male accessory glands saline extract (mrag) and/or human serum albumin (hsa) emulsified in complete freund's adjuvant (cfa) at 6 days after infection. a second immunization was performed 30 days later. four experimental groups were made: group 1- ... | 1987 | 3303977 |
| experimental autoimmune myositis in sjl/j mice. | experimental autoimmune myositis (eam), was produced in sjl/j mice by injection of an emulsion of crude syngeneic muscle homogenate and complete freund's adjuvant (cfa). nearly all injected sjl/j mice showed necrotic muscle fibres associated with infiltrating mononuclear cells, both within the necrotic fibres and in surrounding endomysial connective tissue. the disease in sjl/j mice was confined to skeletal muscle. eight other mouse strains of different major histocompatibility types, similarly ... | 1987 | 3308207 |
| effects of low x-ray doses in saccharomyces cerevisiae. | three strains of saccharomyces cerevisiae with different capacities for repair of radiation damage (rad, rad18, and rad52) have been tested for their colony forming ability (cfa) and growth rates after application of small x-ray doses from 3.8 mgy to 40 gy. there was no reproducible increase in cfa observable after application of doses between 3.8 mgy and 4.7 gy. x-ray doses of 40 gy causing an inactivation of cfa from 90% to 50%, depending on the repair capacity of the strains used, caused a re ... | 1987 | 3317476 |
| tubulointerstitial nephritis by tamm-horsfall glycoprotein or egg white component. | experimental tubulointerstitial nephritis (tin) was induced in wistar rats and new zealand white rabbits by direct intrarenal inoculation of tamm-horsfall glycoprotein (thg) or egg white component (egg white a). after five immunizations of thg or egg white a with complete freund's adjuvant (cfa), direct intrarenal inoculation of thg or egg white a also induced more severe tin in the inoculated kidney. by the 7th day, in 1 of the animals with immunizations and then intrarenal inoculation of thg, ... | 1987 | 3320775 |
| complete freund's adjuvant has a differential amplification action on the induction of diabetes by streptozotocin in various murine strains: cfa amplifies stz in murine diabetes. | the effect of complete freunds adjuvant (cfa) has been investigated on the multi-low-dose streptozotocin (stz) model of diabetes in mice of the h-2b (c57bl/6) and of the h-2k (a/j, cba) genotypes. physiological (glycemia and body weight) and morphological (insulitis) parameters were monitored. stz was used at standard and sub-diabetogenic dose levels (45 and 22.5 mg/kg stz, for five consecutive days respectively) and cfa was given as a single dose (0.1 ml) on the first dosage day. in h-2k mice, ... | 1987 | 3328141 |
| lack of correlation between haemagglutination and adherence to epithelial cells in yersinia pseudotuberculosis. | yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (hep-2) in relation to possession of a virulence (vw) plasmid and to growth conditions. vw-lacking (vw-) bacteria were isolated from ten vw+ strains of each serovar which, after they were grown on cfa plates at 37 degrees c, agglutinated the erythrocytes from five different species. in contrast to the bacteria possessing the plasmid (vw+) half of the vw- bacteria, grown on cfa p ... | 1988 | 3346900 |
| increased production of kurloff cells and accompanying lymphocyte subset changes in immunized guinea-pigs treated with cyclophosphamide and cyclosporin a. | we have used a panel of novel monoclonal antibodies to investigate the influence of cyclophosphamide (cy) and cyclosporin a (csa) on blood and spleen mononuclear cell populations in guinea-pigs immunized with ovalbumin (ova) in complete freund's adjuvant (cfa). female animals received, on day 0, 100 micrograms ova/cfa in each hind footpad and were treated with either cy (300 mg/kg i.p., day -3) or csa (25 mg/kg orally, daily from day 0) or with both drugs. two weeks later, mononuclear cell suspe ... | 1988 | 3350582 |
| c5 gene influences the development of murine myasthenia gravis. | the influence of the c5 gene and c5 deficiency on murine experimental autoimmune myasthenia gravis (eamg) susceptibility was evaluated. two co-isogenic strains, b10.d2/nsn (c5 sufficient) and b10.d2/osn (c5 deficient), which are genetically identical except for the c5 gene locus, were immunized with acetylcholine receptors (achr) in cfa to induce myasthenia gravis. both strains had equivalent concentration of serum autoantibodies to muscle achr and antibodies bound to muscle achr. c5-sufficient ... | 1988 | 3356901 |
| suppression of an established dth response to ovalbumin in mice by feeding antigen after immunization. | experiments were designed to examine whether systemic delayed-type hypersensitivity responses (dth) to ovalbumin (ova) can be suppressed when antigen is fed after immunization, and to investigate the immunological mechanisms involved. a single 25 mg feed of ova given 7 or 14 days after immunization with ova in complete freund's adjuvant (cfa) suppressed the dth response of bdf1 mice, but had no significant effect on the serum igg antibody response. dth suppression was greatest when antigen was f ... | 0 | 3384449 |
| tolerance induction by a poorly arthritogenic collagen ii can prevent collagen-induced arthritis. | collagen type ii (cii)-induced arthritis (cia) can be induced in 78% of b10.riii mice (h2r) by intradermal (id) immunization with cii of bovine origin in complete freund's adjuvant (cfa), whereas immunization with cii of chick origin induces arthritis in less than 5% of these mice. nevertheless, tolerization of b10.riii mice with intravenously injected chick cii renders the animals resistant to induction of cia by immunization with bovine cii. such tolerization can be achieved either by intraven ... | 1988 | 3401929 |
| induction of autoimmunity with dendritic cells: studies on thyroiditis in mice. | the initiation and maintenance of thyroid autoimmunity by professional antigen-presenting cells were assessed by observing thyroiditis and induction of igg antibodies to thyroglobulin (tg). dendritic cells (dc) were purified from spleens of cba mice and t cells removed with anti-thy 1 and complement. some dc were pulsed with 25-500 micrograms/ml of mouse tg in vitro and normal syngeneic mice received injections of 10(5) cells intravenously. in untreated animals only 1 thyroid out of 40 showed a ... | 1988 | 3402103 |
| cellular immune reactions and blood cerebrospinal fluid barrier dysfunction in guinea pigs. | both young and adult strain 13 guinea pigs have been treated with complete freund's adjuvant (cfa). in young animals cfa induced an increase in the lymphocyte cell count in blood and a reversible blood csf barrier impairment. both these effects could be suppressed by the immunosuppressant drug cyclosporin a. in contrast to the young animals treatment with cfa in adult strain 13 guinea pigs influenced neither the lymphocyte cell count nor blood csf barrier function. in young and adult cfa-treated ... | 1988 | 3405372 |
| immunological responsiveness of neonatal a/j mice to isotypic determinants of syngeneic ige. | we have previously shown that adult a/j mice produce high titers of anti-ige with isotypic or idiotypic specificities in response to challenge with a conjugate of klh with syngeneic monoclonal ige. thus, b cells that can synthesize anti-ige are present in the mice. adult mice are unresponsive to unconjugated ige in cfa, suggesting that tolerance exists at the level of t cells. the present study shows that neonatal mice produce anti-ige antibodies in response to unconjugated ige in cfa, but that ... | 0 | 3411291 |
| glycoprobe fructosamine kit used with the rotochem cfa 2000 centrifugal analyzer. | 2012 | 3416449 | |
| [configuration frequency analysis. xxiii. types and syndromes of a higher order]. | configural frequency analysis (cfa) is a method for the detection of significantly high or low frequencies in multivariate contingency tables. as a criterion for the comparisons. cfa uses expected frequencies from log-linear models which reflect assumptions on the association structure of the variables under study. the present paper extends models of cfa such that triplet associations or even higher order associations can be considered. examples use empirical data from research on depression in ... | 1987 | 3451616 |
| active immunization of heifers against prostaglandin f2 alpha: potential as a sterilization vaccine. | four experiments were conducted with 210 heifers in an attempt to develop a sterilization vaccine by active immunization against prostaglandin f2 alpha (pgf2 alpha) and to evaluate feedlot performance following immunization against the combination of pgf2 alpha and estrogens. the objectives were: development of a pgf2 alpha-ovalbumin conjugate that would induce antibody formation when used with complete freund's adjuvant (cfa); comparison of cfa with other adjuvants in relation to pgf2 alpha ant ... | 1987 | 3471746 |
| the effects of pertussis toxin on the induction and transfer of experimental autoimmune uveoretinitis. | experimental autoimmune uveoretinitis (eau), an intraocular inflammatory disease, is induced in experimental animals by immunization with a retinal specific antigen, s-antigen (s-ag), emulsified in complete freund's adjuvant (cfa). the induction of eau is enhanced by treating s-ag-immunized animals with bordetella pertussis. this study examined the effects of a purified component of b. pertussis, pertussis toxin (ptx), on eau induction as well as the mode of action of this toxin. treatment of le ... | 1986 | 3486070 |
| the fate of neonatally injected effector cells of allergic encephalomyelitis. | lymphocytes from rats sensitized with basic protein (bp) plus complete freund's adjuvant (cfa), which produce allergic encephalomyelitis when transferred to adult recipients, fail to induce disease when transferred to 3- to 5-day-old neonatal rats. the transferred cells do, however persist in the recipients and can be revealed by actively challenging with bp-cfa later in adult life. challenge leads to a significantly earlier onset of disease than is seen in control animals. we report here that t ... | 1986 | 3486457 |
| immune sensitization to syngeneic organ-specific intestinal antigens in the lewis rat. | in human chronic inflammatory bowel disease involving mucosal epithelium, sera and lamina propria mononuclear cells are reactive with cell surface components isolated from gut epithelial cells. to define a model system in which the disease-inducing potential of such immune factors could be rigorously evaluated, we sought to immunologically sensitize inbred murine strains to syngeneic colonic epithelial cell-associated components (ecac-c), to define precise in vivo and in vitro conditions to opti ... | 1986 | 3489532 |
| inhibition of an ige response by secondary b cells of a different isotype. | we found that the synthesis of ige anti-ars antibodies is strongly inhibited by the presence of secondary non-ige-producing cells that are specific for the ars hapten. such b cells can be induced by inoculation of a protein-ars conjugate in cfa. the effect is seen after inoculation of ova-ars in cfa followed by klh-ars in alum, or, more convincingly, after adoptive transfer of b cells induced by antigen in cfa. dose-response data indicated that inhibition can be effected by b cells containing no ... | 0 | 3491175 |
| in vitro cell-mediated and complement-mediated cytotoxicity to murine testicular cells. | male balb/c mice at 8 to 14 weeks of age were divided into three groups: group 1 was immunized with an emulsion of testicular cells (tcs) (10(7)/mouse), complete freund's adjuvant (cfa), and extract of bordetella pertussis (bp); group 2 was given cfa and bp injections; and group 3 was given sterile saline injections. suspensions of tcs and spleen cells (scs) from each mouse were prepared 4 weeks after the first immunization for a cytotoxic t lymphocyte (ctl) assay. for the antibody-dependent cel ... | 1986 | 3491547 |
| delayed type hypersensitivity (dth) to type ii collagen (cii) in dba-1 mice. | sensitization of dba-1 mice with type ii collagen (cii) in complete freund's adjuvant can cause polyarthritis. a possible link between cii-induced arthritis and delayed type hypersensitivity (dth) has been suggested, so we decided to investigate the susceptibility of dba-1 mice to cii induced dth reactions. the mice were primed with a dose of 10 micrograms cii i.p. 4 days before challenging with 40 micrograms cii in the ear. swelling was measured 48 h later and was found to be reproducible. resp ... | 0 | 3491709 |
| functional mapping of ventricular contraction and phase using single-photon emission computed tomography (spect). | a method for functional mapping of ventricular contraction and phase was developed using gated blood-pool spect. parameters of contraction and phase were calculated using length-based and count-based fourier analyses. in length-based fourier analysis (lfa), percentage-shortening and length-based phase were calculated based on the changes of lengths from a ventricular centre to edges. in count-based fourier analysis (cfa), phase and amplitude were also calculated using serial tomographic phase im ... | 1986 | 3494967 |
| unresponsiveness of mrl/mp-lpr/lpr mice to antigen given subcutaneously in adjuvant: partial restoration of response after local injection of b cells. | mrl/lpr mice were used as a model for seeking information on the role of b cells as antigen-presenting cells in vivo. in confirmation of the finding of other workers, mrl/lpr mice with pronounced lymphadenopathy failed to undergo t cell priming in lymph nodes (ln) after s.c. injection of keyhole limpet hemocyanin (klh) in complete freund's adjuvant (cfa): thus, in contrast to normal mice or young mrl/lpr mice, the ln cells from older mrl/lpr mice failed to give secondary t proliferative response ... | 2011 | 3496379 |
| delayed-type hypersensitivity to transplantation antigens in mice and its investigation under conditions of allograft and xenograft tolerance. | methods of induction of delayed-type hypersensitivity (dth) to transplantation alloantigens and xenoantigens in mice were developed. they include the s.c. sensitization of mice with spleen cells (5 x 10(6) xenogeneic cells in cfa or 1 x 10(7) allogeneic cells) followed 5 days later by testing of the reactions. reactions induced to alloantigens and to xenoantigens were the dth reactions: they reached a maximum 24 h after testing, were mediated by t lymphocytes, were not transferred to intact reci ... | 1987 | 3497824 |
| the effect of aging on the induction of experimental autoimmune thyroiditis. | the effect of age on the ability to elicit the various immune functions comprising experimental autoimmune thyroiditis in mice has been examined. compared with young mice (2 to 3 mo), cba/caj and a/j aged mice (20 to 30 mo) show a drastic reduction in their ability to develop circulating antibody after injection of mouse thyroglobulin (mtg) or mouse thyroid extract (mte) in complete freund's adjuvant (cfa). delayed-type hypersensitivity responses were also depressed, as well as the ability of ag ... | 1987 | 3497973 |
| experimental allergic orchitis in mice: iv. preliminary characterization of the major murine testis specific aspermatogenic autoantigen(s). | active experimental allergic orchitis (eao), characterized by inflammation of the testes (autoimmune orchitis), aspermatogenesis, epididymitis and vasitis was induced in mice using a panel of tissue antigens as immunogens. immunization with allogeneic murine tissue homogenates emulsified in complete freund's adjuvant (cfa) accompanied by the injection of pertussigen revealed that only adult murine testicular and epididymal homogenates are capable of eliciting murine eao. all other tissue antigen ... | 1987 | 3501013 |
| [effect of intratumor macrophages on the growth of a rat sarcoma]. | the removal of active phagocytic cells (cfa) from suspensions of a rat sarcoma (s-e 100) caused a decrease in tumor development in "m" line rats; consequently, we postulated that the macrophage (m phi) population infiltrating the tumor might possess inhibitory functions. in the present paper we investigate whether the effect of cfa is a general one or whether it is dependent on the interaction between m phi infiltrating the tumor and the recipient. s-e 100 was inoculated in "m" line rats (s-e 10 ... | 1987 | 3503311 |
| plasmids coding for colonization factor antigens i and ii, heat-labile enterotoxin, and heat-stable enterotoxin a2 in escherichia coli. | colonization factor antigens i and ii (cfa/i and cfa/ii) are important in the pathogenesis of diarrhea in humans caused by some enterotoxigenic escherichia coli (etec). plasmid dna from 16 cfa/i+ and five cfa/ii+ etec were examined by southern blot analysis with enterotoxin gene probes and were compared with plasmid dna from derivatives of the same etec that had lost the ability to produce these colonization factors. among the 16 cfa/i+ etec strains, the loss of cfa/i was accompanied by the loss ... | 1986 | 3510984 |
| local and systemic antibody responses to naturally acquired enterotoxigenic escherichia coli diarrhea in an endemic area. | fifteen patients hospitalized with acute, watery diarrhea and with enterotoxigenic escherichia coli (etec) detected from stool samples were studied to evaluate the extent to which natural etec diarrhea induces local and systemic antibody responses to e. coli heat-labile toxin (lt), homologous lipopolysaccharide (lps), and colonization factors (cfa/i and cfa/ii). specific iga and igg antibodies to lt, cfa i and ii, and each patient's homologous lps were determined by elisa in serum, saliva, breas ... | 1986 | 3512729 |
| characterization of escherichia coli mutants completely defective in synthesis of cyclopropane fatty acids. | the synthesis of cyclopropane fatty acids (cfa) in bacteria represents a biochemically and physiologically unique membrane modification whose importance for the cell remains unknown, despite extensive study of a cfa- mutant of escherichia coli and of the cloned cfa gene. recently we reported the isolation of new cfa- mutants (d. w. grogan and j. e. cronan, jr., mol. gen. genet. 196:367-372, 1984). molecular-genetic and biochemical analysis indicated that these were null mutants of the e. coli cf ... | 1986 | 3519583 |
| opsonic antibodies evoked by hybrid peptide copies of types 5 and 24 streptococcal m proteins synthesized in tandem. | the protective immunogenicity of a hybrid peptide containing tandem copies of types 5 and 24 epitopes was investigated. carboxy-terminal peptides of the cyanogen bromide-derived fragment 7 (cb7) of type 24 m protein were chemically synthesized, and then extended to include the first 20 residues of the amino-terminus of type 5 m protein. when emulsified in cfa and injected into rabbits without conjugation to a carrier, each of the synthetic hybrid peptides, designated s-m5(1-20)-s-cb7(23-35)c and ... | 1986 | 3519829 |
| experimental systemic amyloidosis induced by immunization with syngeneic organ extracts in mice. | systemic amyloidosis was induced consistently in mice by intramuscular injection of syngeneic organ (liver and kidney) extracts mixed with cfa six times at weekly intervals. syngeneic organ extract with cfa also induced amyloidosis of a lesser degree. all three strains of mice (c57bl/6, c3h/he, and balb/c) injected with a syngeneic liver extract mixed with cfa developed systemic amyloidosis; the most prominent amyloid deposition occurred in c57bl/6 (b6) mice, followed by c3h/he and balb/c. the a ... | 1986 | 3519830 |
| trauma of the extremities: prospective comparison of digital and conventional angiography. | digital subtraction angiography (dsa) and conventional film angiography (cfa) were compared prospectively in 50 patients with trauma of the extremities. each patient underwent both procedures with comparable imaging parameters and injections. three angiographers reviewed the angiograms for the presence of seven angiographic signs of arterial injury. the two modalities showed pseudoaneurysms, arteriovenous fistulas, vessel displacement, occlusion, and focal narrowing equally well. cfa was superio ... | 1986 | 3520645 |
| protection of c3h/hej mice from lethal salmonella typhimurium lt2 infection by immunization with lipopolysaccharide-lipid a-associated protein complexes. | c3h/hej mice were immunized intraperitoneally (i.p.) with lipopolysaccharide (lps)-lipid a-associated protein (lap) complexes or with purified protein-free lps prior to lethal i.p. or intravenous salmonella typhimurium lt2 challenge. our results demonstrated that these salmonella-hypersusceptible mice can be effectively protected against 1,000 100% lethal doses of s. typhimurium lt2 (i.e., 1,000 viable bacteria) administered by intravenous challenge when previously immunized with lap-lps complex ... | 1986 | 3531011 |
| effect of active immunization against lhrh or lh in boars: reproductive consequences and performance traits. | forty crossbred boars were equally divided into eight groups at birth. four groups were immunized (200 micrograms/boar) at 12 wk of age against either luteinizing hormone-releasing hormone (lhrh) conjugated to human serum globulin (lhrh-hsg) in complete freund's adjuvant (cfa), lhrh-hsg in muramyldipeptide adjuvant (pep), procine luteinizing hormone (lh) conjugated to hsg (plh-hsg) in cfa or ovine lh (olh) in cfa. equal doses of boosters were given in either pep or incomplete freund's adjuvant ( ... | 1986 | 3531138 |
| hemadsorption and enzyme-linked immunosorbent assay nitrocellulose replica methods for identification of colonization factor antigen (cfa)-positive escherichia coli colonies and for isolation of cfa-negative mutants. | methods were developed that allow demonstration of individual colonies carrying colonization factor antigen (cfa) i or cfa/ii or e8775-type antigen in mixed bacterial cultures on solid media. these methods are based on mannose-resistant hemadsorption or cfa enzyme-linked immunosorbent assay (elisa) on nitrocellulose replicas of the cultures allowing simultaneous analysis of up to 200 colonies per plate. the sensitivity and specificity of the cfa elisa nitrocellulose replica method were 97 and 99 ... | 1986 | 3533978 |
| the immunopathology of acute experimental allergic encephalomyelitis. iv. an ultrastructural immunocytochemical study of class ii major histocompatibility complex molecule (ia) expression. | cell surface expression of class ii major histocompatibility complex (ia) molecules is required for antigen recognition by t cells. to determine the ultrastructural cellular distribution of ia molecules in the autoimmune disease model acute experimental allergic encephalomyelitis (eae) we studied central nervous system (cns) tissues from adult strain 13 guinea pigs (gp). experimental allergic encephalomyelitis was induced by sensitization with gp spinal cord homogenate in complete freund's adjuv ... | 1987 | 3549982 |
| attempts to localize the defect in dysgammaglobulinemia of um-b19 chickens by studying the effect of immunomodulating substances on immunoglobulin and antibody production. | the extreme decreased levels of igg in dysgammaglobulinemic um-b19 chickens are linked with decreased antibody activity. antibody activity to t-dependent (although diminished) and t-independent antigens is present but is restricted to igm and iga antibodies. complete freund's adjuvant enhances the existing isotype pattern of serum immunoglobulins and antibodies. the antibody response to a "t-independent" antigen (b. abortus) is greatly increased by cfa in dysgammaglobulinemic chickens. beside th ... | 1987 | 3564361 |
| polycythaemia rubra vera and water-induced pruritus: blood histamine levels and cutaneous fibrinolytic activity before and after water challenge. | we studied blood histamine activity (ha) and cutaneous fibrinolytic activity (cfa) in a patient with polycythaemia rubra vera (prv) and water-induced pruritus, before and after water exposure. the results suggest that the water-induced itching in prv is associated with an increase in ha. in addition, markedly increased levels of cfa were found both before and after water exposure. these findings have been previously reported in patients with aquagenic pruritus (ap) but not in patients with prv. ... | 1987 | 3567071 |
| analysis of human ascites effect on clonogenic growth of human tumor cell lines and nrk-49f cells in soft agar. | we studied the factors that control the capacity of tumor cells to grow in soft agar. and, we analyzed the effect of cell-free ascites (cfa) obtained from ovarian cancer patients in combination with various media on the cloning efficiency of h-134 and ovcar-3nu ovarian carcinoma cell lines and the widr colon carcinoma cell line. seven batches of cfa consistently enhanced the soft agar growth of tumor cells more efficiently than tested sera. the addition of charcoal-treated bovine serum albumin ( ... | 1987 | 3572052 |
| cryptogenic fibrosing alveolitis in children. | cryptogenic fibrosing alveolitis (cfa) in children is supposedly a rare diffuse lung disease characterized clinically by tachypnoea, cough, poor weight gain, and cyanosis. histologically, it is characterized by thickening of alveolar walls and infiltration of the alveolar septae with mononuclear cells and fibrous tissue. the condition is more acute in children, with fulminant course. ten children were diagnosed as having cfa out of three hundred children seen over a period of 3 years. diagnosis ... | 1987 | 3578926 |
| significance of immune responses to mucosal carcinogens: a hypothesis and a workable model system. | carcinogens must enter the tissues before neoplastic transformation of cells can occur. the present report describes the 2-aaf model system being used to test our hypothesis that development of mucosal immunity to carcinogens will interfere with their passage through mucosal epithelial cells. the 2-aaf model is ideal for testing this hypothesis. 2-aaf is a carcinogen which passes through the intestinal epithelium following oral administration, is transported to the liver and is there metabolized ... | 1986 | 3601874 |
| toxicity of chloroprocaine and sodium bisulfite on human neuroblastoma cells. | the effects of commercial and crystalline solutions of chloroprocaine (cp) (1.6 x 10(-3)-0.4 x 10(-3)m) and sodium bisulfite (sb) (0.8 x 10(-3)-0.08 x 10(-3)m) were studied on the multiplication of human neuroblastoma cells. these cells were chosen because of putative neurotoxicity associated with cp; cell multiplication (measured as colony-forming ability-cfa) was studied because cfa is a reliable in vitro test for drug toxicity at the cellular level. cfa was dramatically reduced (86%) after 20 ... | 1987 | 3631590 |
| experimental allergic encephalomyelitis in rhesus monkeys: i. immunological parameters in eae resistant and susceptible rhesus monkeys. | immunological parameters in rhesus monkeys, resistant and susceptible to experimental allergic encephalomyelitis (eae), were studied. monkeys immunized with complete freund's adjuvant (cfa) alone and eae resistant monkeys immunized with a low dose of bovine brain homogenate emulsified in cfa did not show significant fluctuations in numbers of granulocytes, lymphocytes and lymphocyte subsets (cd4; cd8; gm13, a subset of cd8) and anti-brain homogenate antibody titres remained low. eae susceptible ... | 1987 | 3652511 |
| [use of the küchenhoff continuity correction of the lehmacher cfa test in search of types of sociogenic behavior disorders]. | to avoid anticonservative rejections of cfa null hypotheses when using lehmacher's test küchenhoff suggested to apply a continuity correction. this correction reduces the differences between observed and expected cell frequencies by a constant. the present paper discusses this variant of lehmacher's test. application of the correction for continuity in the search for types of sociogene neuropathology are presented. the discussion covers the effectivity of cfa tests in connection with holm's meth ... | 1987 | 3660914 |
| liposome as an adjuvant for the production of estradiol antibodies. | mice immunized with liposome associated estradiol-bovine serum albumin conjugate (e2-bsa) showed a significantly higher estradiol-specific immune response than did mice injected with the free conjugate. the conjugation of estradiol with bsa is imperative, since injection of liposomes with estradiol in the lipid bilayer was found to be ineffective in inducing an immune response. entrapment of e2-bsa in neutral and positively charged liposomes was found to be a potent mode of immunostimulation, ev ... | 1987 | 3666803 |
| alterations in rat renal glucose transport following in vivo use of freund's adjuvant. | alterations in rat renal glucose transport following in vivo use of freund's adjuvant were examined. lewis-brown norway rats were placed in four separate injection groups: tubular basement membrane plus adjuvant [complete freund's adjuvant (cfa) plus pertussis]; adjuvant (cfa plus pertussis); cfa only; or pertussis only. renal handling of glucose was assessed 14 days after a single injection. no in vivo changes were noted. no histologic differences among groups were noted. however, brush border ... | 1986 | 3714353 |
| ultrastructural study of central nervous system demyelination in galactocerebroside sensitized rabbits. | the rabbit eye model was used to investigate the role of delayed type hypersensitivity and of circulating antigalactocerebroside antibody in primary demyelination. delayed type hypersensitivity reaction to tuberculin was induced within the retinal myelinated layers by injecting purified protein derivative into the vitreous of rabbits that were previously immunized with complete freund's adjuvant (cfa) alone, or galactocerebroside (gc) in cfa. the morphological features of the myelinated zones we ... | 1986 | 3724066 |
| [experimental study of soft agarose colony formation assay of renal carcinoma]. | several problems have been pointed out in colony formation assay (cfa) developed by hamburger and salmon. we studied those using 87 fresh renal carcinoma, comprising of 72 renal cell carcinoma and 15 transitional cell carcinoma of renal pelvis. results are as follows: pure single cell suspension was difficult to be yielded from these carcinoma. our result suggests that cell suspension contains various sized growth unit as well as greater than or equal to 60 microns growth unit. presence of subst ... | 1986 | 3729441 |
| heterogeneity of xeroderma pigmentosum (xp); variability and stability within and between the complementation groups c, d, e, i and variants. | thirty-two cases of xeroderma pigmentosum (xp) of the complementation groups c (7), d (12), e (3), i (2) and 8 variants are analyzed biochemically and clinically. there is some congruence of the cellular defects (uds, cfa, sce) and the clinical severity of the skin symptoms. despite the large clinical variability within and between the complementation groups, several clinical features are to be attributed to one group or another. the most striking observation is the predominance of lmm in the d ... | 1986 | 3748852 |
| cyclic fatty esters: synthesis, characterization, and lipolysis of isomeric triglycerides of 9-(6-propyl-3-cyclohexenyl)-(z)8-nonenoic acid. | triglycerides of a model cyclic fatty acid (cfa) 9-(6-propyl-3-cyclohexenyl)-(z)8-nonenoic acid (ia) were synthesized for biological and toxicity evaluations. the monoacid triglyceride ii (cycycy) was interesterified with triolein (ooo) to obtain mixtures of diacid triglycerides: iii (oocy), iv (ocyo), v (ocycy), and vi (cyocy). the interesterification mixtures were separated by preparative hplc into two 'critical pairs' of isomeric triglycerides. triglycerides iii-vi were synthesized and a 13c- ... | 1986 | 3757149 |
| the effect of dose, route, number of injections and the use of adjuvant on the clearance of and immune response to, haemocyanin following injection into brown trout (salmo trutta). | the primary and secondary immune responses were investigated in trout injected with haemocyanin (hcn) intramuscularly (im) or intraperitoneally (ip), with or without adjuvant. hcn was detected in the blood 30 min after injection and clearance times varied after one injection from 8 to 56 days. fish given two and three injections cleared the antigen faster. precipitins against hcn were first detected 21 to 30 days after injection and were still present on day 56. however, antibodies detectable by ... | 1986 | 3765340 |
| long-term evolution of bcg- and cfa-induced granulomas in rat lungs. correlation of histologic features with cells in bronchoalveolar lavage samples. | granulomatous inflammation was induced in the lungs of rats for assessment of the suitability of this animal species for long-term study of granuloma development and resolution and for comparison of the histologic changes with the cellular profile in bronchoalveolar lavage (bal) samples. it was found that after a single intravenous injection of bacillus calmette-guérin (bcg) organisms suspended in saline-0.01% triton, complete freund's adjuvant (cfa), or bcg suspended in cfa (bcg + cfa), distinc ... | 1986 | 3777133 |
| immune protective mechanisms during pregnancy. i. cell-mediated immunity against listeria monocytogenes in pregnant mice. | characteristics of protective mechanisms during pregnancy were investigated using neonatally thymectomized (ntx) and/or pregnant mice infected with sublethal doses of listeria monocytogenes, of which the explosive growth at an early phase of 2 or 3 days after infection is prevented by non-immune macrophages, and complete elimination at a late phase from 4 to 10 days after infection is attributed to the augmented functions of macrophages in co-operation with lymphokine-producing sensitized t lymp ... | 1986 | 3793136 |
| the value of serial bronchoalveolar lavages in assessing the clinical progress of patients with cryptogenic fibrosing alveolitis. | this study tested the hypothesis that changes in bronchoalveolar lavage (bal) inflammatory cell profiles reflect the clinical progress of patients with cryptogenic fibrosing alveolitis (cfa) when followed for substantial periods of time with or without any form of treatment. two or more bronchoalveolar lavages (mean, 3; maximum, 5) were performed on each of 32 patients who were followed for periods as long as 7 yr (mean, 2.5 yr, median, 4 yr). the majority were studied before and during treatmen ... | 1987 | 3800151 |
| the regulation of tissue eosinophilia. v. induction of lymphocyte-derived eosinophil chemotactic inhibitory factor production by a macrophage product from complete freund's adjuvant-treated guinea pigs. | splenic t lymphocytes of complete freund's adjuvant (cfa)-treated guinea pigs spontaneously produce a lymphokine, eosinophil-directed chemotactic inhibitory factor (ecif), which selectively inhibits the chemotactic response of the treated eosinophils to a certain chemotactic lymphokine for eosinophils. in the present paper, induction of spontaneous ecif production was examined in cfa-treated guinea pigs. although short-time (3-hr) cultured supernatants of cfa-treated spleen cells exhibited littl ... | 1987 | 3802211 |
| genetic regulation of testosterone-induced immune suppression. | genes in the major histocompatibility complex (h-2) of the mouse control several immune functions as well as various facets of testosterone (te) physiology. in order to study the genetic control of te-induced immune suppression, complete freund's adjuvant (cfa; containing mycobacteria tuberculosis) was administered parenterally to several mouse strains differing at the h-2 complex which were either sham- or te-treated. the specific lymphocyte proliferative response to purified protein derivative ... | 1987 | 3802214 |
| complementation of cell and serum growth factors during proliferation of tumour cells in semisolid medium. | foetal bovine serum (fbs) and the preparation ultraser (lkb, sweden) contain more growth factors (gf) necessary to promote proliferation of cells in soft agar than the sera from newborn and adult animals. it was found that non-dialysable gfs are produced during growth of cells in semisolid medium. the dependence of colony-forming efficiency (cfe) on plating cell dose (pcd) was studied in 11 cell lines at varying concentrations of fbs. cell proliferation depended on both serum and cell gfs. the m ... | 1986 | 3803626 |
| anti-cholera response elicited by a completely synthetic antigen with built-in adjuvanticity administered in aqueous solution. | some synthetic peptides derived from the b subunit of cholera toxin, conjugated to tetanus toxoid have been shown by us to be efficient when administered in complete freund's adjuvant (cfa), in induction of anticholera toxin response with neutralizing capacity. when the peptide ctp3, corresponding to residues 50-64 within the sequence of the b subunit, was attached to the hardly immunogenic a-l (multichain poly-dl-alanine) the resulting conjugate did not lead to a significant anti-peptide respon ... | 1986 | 3804383 |
| effects of the adjuvants sgp and quil a on the induction of experimental autoimmune thyroiditis in mice. | in the present study, two adjuvants, sgp and quil a, were assessed for their ability to induce experimental autoimmune thyroiditis (eat) in mice. sgp (a synthetic copolymer of starch, acrylamide, and sodium acrylate) and quil a (a plant saponin) were compared with lipopolysaccharide (lps) and complete freund's adjuvant (cfa) given together with mouse thyroglobulin (mtg) for their ability to induce eat in cba/j mice. immunization with mtg and lps, mtg and cfa, or mtg with sgp was effective in ind ... | 1987 | 3815534 |
| effects of natural or synthetic microbial adjuvants on induction of autoimmune thyroiditis. | natural and synthetic adjuvants of microbial origin were compared for their capacity to potentiate the induction of experimental autoimmune thyroiditis (eat) with the autoantigen mouse thyroglobulin (mtg). regardless of the immunomodulator used, severe thyroiditis was observed only in eat-susceptible strains of the k haplotype and not in eat-resistant strains of the d haplotype. compared to phenol-extracted lipopolysaccharide, a potent adjuvant for enhancing eat induction, phthalyl-substituted, ... | 1985 | 3839208 |
| preparation for hapten help by glucan, muramyl dipeptide, and its l-ala-glycerol-mycolate derivative. | previously, we reported that one of the factors that determines whether or not an animal will be prepared for hapten help after priming is the type of adjuvant used. the present work was undertaken, therefore, to determine which of a diverse variety of adjuvants or biological response modifiers would be effective. they included freund's complete (cfa) and incomplete (fica) adjuvants, particulate glucan, muramyl dipeptide (mdp), and its l-ala-glycerol-mycolate derivative. help by the azobenzenear ... | 1985 | 3861748 |
| the regulation of tissue eosinophilia. iii. in vitro production of eosinophil-directed chemotactic inhibitory factor by t lymphocytes of complete freund's adjuvant-treated guinea-pigs. | guinea-pig spleen cells treated with complete freund's adjuvant (cfa) produce eosinophil-directed chemotactic inhibitory factors (ecif). the inhibition is selective for the response of eosinophils to delayed ecf-a, which had been isolated from 24-hr-old inflamed skin lesions induced by dnp-ascaris extract in sensitized animals and had been confirmed as being a t lymphocyte-derived lymphokine. ecif activity is absorbed by incubation with eosinophils, but not with macrophages or neutrophils. the c ... | 1985 | 3873403 |
| thymosin alpha 1-induced modulation of cellular responses and functional t-cell subsets in mice with experimental autoimmune thyroiditis. | the effects of ta-1, a peptide constituent of thymosin fraction 5, were studied on murine autoimmune thyroiditis using two congenic strains of mice, b10.br (br) and b10.d2 (d2), which are sensitive and resistant to experimental autoimmune thyroiditis (eat) induction, respectively. eat was induced by either 2 weekly iv injections of mouse thyroglobulin with adjuvant lipopolysaccharide (lps) or intradermal injection of thyroglobulin mixed with complete freund's adjuvant (cfa). the criteria for ind ... | 1985 | 3873993 |
| transfer of experimental autoimmune thyroiditis to normal syngeneic mice by injection of mouse thyroglobulin-sensitized t lymphocytes after activation with concanavalin a. | induction of autoimmune thyroiditis in normal syngeneic cba/j mice was achieved by injection of 72-hr concanavalin a (con a)-induced lymphoblasts from donor mice which had been immunized with mouse thyroglobulin (mtg) emulsified with complete freund's adjuvant (cfa). injection of lymph node or spleen cells, or frequent injection of serum taken from mice with autoimmune thyroiditis failed to transfer appreciable thyroiditis to recipient mice. selection by treatment of incubated cells with monoclo ... | 1985 | 3874026 |
| chronic relapsing experimental allergic encephalomyelitis in the lewis rat. | chronic relapsing experimental allergic encephalomyelitis (cr-eae) was induced in rats with an emulsion of guinea-pig spinal cord tissue (gpsc) in complete freund's adjuvant (cfa) enriched with mycobacterium tuberculosis h37ra (tbc). 78% of the sensitized rats developed a cr-eae showing 2 to 3 clinical relapses during the first 40 days. after 60-80 days, approximately half of the rats with cr-eae had a further relapse which was followed by complete recovery in only 35% of the cases. the remainin ... | 1985 | 3877741 |
| enterotoxin production, presence of colonization factor antigen i, and adherence to hela cells by escherichia coli o128 strains belonging to different o subgroups. | strains of three subgroups of escherichia coli o128 were studied. enterotoxin production was observed in 30 (91%) o128ac strains, whereas strains of subgroups o128ab and o128ad were not toxigenic. cfa/i was only found in two serotypes of subgroup o128ac, all of them producing heat-stable enterotoxin except for one which produced both toxins. none of the strains studied produced cfa/ii. in a binding test with hela cells, localized adherence was found only in strains of subgroup o128ab; diffuse ad ... | 1985 | 3880728 |
| survey in vanuatu on enterotoxigenic escherichia coli in children and infants with and without acute diarrhea. | we have studied the incidence of enterotoxigenic escherichia coli (etec) strains isolated from infants with and without diarrheal diseases in vanuatu, south pacific. over a period of 5 months we have isolated enterotoxigenic e. coli strains from 29 (26.6%) of 109 children with acute diarrhea and from 13 (21.6%) of 60 children of the control group. in the group with diarrhea, 7 (6.4%) strains released heat-labile toxin, 7 (6.4%) released heat-stable toxin, and 15 (13.7%) produced both heat-labile ... | 1985 | 3886696 |
| [comparative study of adherence to rabbit enterocytes, presence of colonization factors cfa/i and cfa/ii and toxinogenesis of 55 strains of escherichia coli]. | fifty-five strains of escherichia coli isolated from 51 faeces of melanesian children with acute diarrhoea in new caledonia were studied; three diarrhoeas were bloody. for each strain, haemagglutination type, adhesion to rabbit enterocytes, serotype, production of heat-labile (lt) or heat-stable (st) toxins and identification of colonization factor antigens cfa/i or cfa/ii were determined. we identified 48 strains able to attach to rabbit enterocytes; 27 produced enterotoxins (21 strains lt+ and ... | 1985 | 3890696 |
| [prevalence of the enterotoxigenic escherichia coli colonization factors cfa/i, cfa/ii and e8775 isolated in the south pacific (new caledonia, republic of vanuatu and wallis and futuna)]. | we tested the expression of adherence properties of enterotoxigenic escherichia coli (etec) strains isolated in new-caledonia, vanuatu and wallis and futuna by examining for the presence of colonization factor e8775 using an agglutination test and an immuno-diffusion technique with specific antisera. approximately 19% of etec strains possessed cfa/i and 21% a cfa/ii. the e8775 antigen was found on 1.8% of the strains. this last factor was found on strains of the serogroup 025 from vanuatu. two s ... | 1985 | 3896547 |
| enterotoxigenic escherichia coli strains belonging to a new serogroup, escherichia coli o166. | thirty-two strains of escherichia coli belonging to a new o group, o166, were examined. twenty-one strains had the flagella antigen h27, five had the h15 antigen, five had the h7 antigen, and one was nonmotile. all the h27 strains and the nonmotile strain produced heat-stable enterotoxin but not heat-labile enterotoxin. all the h7 strains produced heat-labile enterotoxin but not heat-stable enterotoxin. the remaining strains were nonenterotoxigenic. none of the strains possessed colonization fac ... | 1985 | 3902874 |
| adjuvant specific immunotherapy of resectable squamous cell lung carcinoma. analysis at the eighth year. | from june 1976 to june 1981, 86 patients with resectable (stage i and ii) squamous cell lung carcinoma were entered into a randomized controlled study with three arms: control group - no treatment postoperatively. specific immunotherapy group - three monthly doses of 500 micrograms of tumor associated antigen (taa) emulsified with complete freund's adjuvant (cfa). nonspecific immunotherapy group - three monthly doses of cfa emulsified in saline. all the patients in the study received skin tests ... | 1985 | 3904977 |
| enteroadhesion fimbriae and enterotoxin of escherichia coli: genetic transfer to a streptomycin-resistant mutant of the gale oral-route live-vaccine salmonella typhi ty21a. | an enterotoxigenic escherichia coli plasmid encoding colonization factor antigen (cfa) i fimbriae and heat-stable toxin was transferred into a streptomycin-resistant mutant of the salmonella typhi gale strain ty21a (a live attenuated oral typhoid vaccine). the virulence plasmid-carrying transconjugants produced cfa i fimbriae and heat-stable toxin. the marked production of cfa i fimbriae was observed even in a vaccine medium for ty21a. the data lead to a new type of potential live oral vaccine, ... | 1985 | 3905619 |
| [epidemic of gastroenteritis in noumea (new caledonia) caused by an enterotoxinogenic strain of escherichia coli (0l26:b16) believed to be enteropathogenic]. | a strain of enteropathogenic escherichia coli 0126:b16 has been isolated in fifteen children and one adult during a severe outbreak. one infant is dead. the strain produced heat-stable enterotoxin, attach to rabbit enterocytes but did not have colonization factor antigen cfa/i or cfa/ii. its hemagglutination type was the same that the e. coli h10407, cfa/i+. it presented a resistance at eight antibiotics and, with the loss of enterotoxigenicity, there was a loss of resistance at ampicillin and o ... | 1985 | 3906346 |
| enterotoxigenic escherichia coli in a population of infants with diarrhea in chile. | the incidence of enterotoxigenic escherichia coli (etec) was investigated in 95 e. coli strains isolated from 48 infants with diarrhea in santiago, chile. by using standard biological assays and dna-dna hybridization procedures, etec was found in 31.2% of the cases: 14 strains produced heat-stable enterotoxin (st) only, three strains produced heat-labile enterotoxin (lt) and st, and two strains produced lt only. dna probes detected all enterotoxin producers except one st-producing strain. the st ... | 1985 | 3908470 |
| the skin inflammatory response of the badger (meles meles). | twenty-five badgers, captured in an area where they had been implicated in outbreaks of bovine tuberculosis, received intradermal inoculations of control medium, 150 micrograms phytohaemagglutinin (pha), 40 units streptokinase/10 units streptodornase (sk/sd), 200 micrograms purified protein derivative of mycobacterium bovis (ppd), freund's incomplete adjuvant (ifa), and freund's complete adjuvant (cfa), each in 0.1 ml of inoculum. the reactions were assessed by skinfold thickness and skin histol ... | 1985 | 3910074 |
| flavor avoidance expressed in grooming by pine voles (microtus pinetorum): importance of context and hormonal factors. | pine voles (microtus pinetorum) were given sipper tubes containing saccharin solution, and after drinking, were injected with 0.15 m licl. subsequently, two-sipper tests were given to assure the presence of conditioned flavor avoidance (cfa), and then animals were presented with saccharin in carboxymethyl-cellulose on their own fur (autogrooming), or on the fur of cagemates (allogrooming). cfa was expressed in two-sipper tests, and during allogrooming of material from a cagemate's fur, but not d ... | 1985 | 3912780 |
| agglutination and fusion of globoside gl-4 containing phospholipid vesicles mediated by lectins and calcium ions. | we have investigated the interaction of five n-acetylgalactosamine (galnac) specific lectins with the glycosphingolipid globoside gl-4, inserted into phospholipid vesicles composed of phosphatidyl-ethanolamine and phosphatidic acid, with respect to their ability to induce vesicle agglutination, fusion, and destabilization. the following lectins were used: soybean agglutinin (sba); sophora japonica agglutinin (sja); helix pomatia agglutinin (hpa); ricinus communis agglutinin ii (rcaii); and codiu ... | 1985 | 3922401 |
| genetic analysis of anti-tbm disease in the norway rat. | the anti-tubular basement membrane (tbm) disease was found in own kidneys of bn, but not bn.1b rats, after immunization with bp.1n (or bp) allogeneic kidney homogenate in cfa. both humoral [anti-tbm reaction assessed by direct immunofluorescence (if)] and cellular response to tbm antigen (tubulointerstitial nephritis and giant cell infiltration) appeared to be controlled by an mhc-linked ir gene(s). in the present experiments all of the 54 rt1b/rt1n heterozygous bc rats (bn.1b x bn) x bn.1b were ... | 1985 | 3930301 |
| enzyme-linked immunosorbent assays for the detection of adhesion factor antigens of enterotoxigenic escherichia coli. | two hundred forty-four specimens of escherichia coli isolated in bangladesh and thailand and identified as enterotoxin producers were tested for the presence of adhesion antigens by mannose-resistant hemagglutination, immunodiffusion, and enzyme-linked immunosorbent assays (elisas). specific antisera to the antigens colonization factor antigen (cfa)/i, cfa/ii (consisting of coli surface antigens [cs] 1, 2, and 3), and putative colonization factor antigen (pcf) 8775 (consisting of cs4, 5, and 6) ... | 1985 | 3934290 |
| presence of colonization factor antigens on fresh isolates of fecal escherichia coli: a prospective study. | in dhaka, bangladesh, fresh isolates of escherichia coli from 197 patients with diarrhea were investigated for production of enterotoxin and possession of colonization factor antigen (cfa) i or ii. enterotoxigenic e. coli (etec) was isolated from 34% of the patients, and of the 67 enterotoxin-positive strains, 75% carried cfas. among 68 healthy control persons no strains positive for both enterotoxin and cfa were found. the cfas in general were restricted to certain serotypes of e. coli. in a su ... | 1985 | 3934291 |
| biochemical evidence for an increased and progressive deposition of collagen in lungs of patients with pulmonary fibrosis. | to assess the role of changes in lung collagen in pulmonary fibrosis, the content of this protein was measured in biopsy and autopsy lung from patients with cryptogenic fibrosing alveolitis (cfa), a fibrotic lung disorder of unknown cause. the collagen concentration was measured in lung samples from 21 patients with cfa (14 autopsy and seven open-lung biopsy) and 17 normal subjects; total lung collagen was determined in the right lung of 10 patients who died from cfa and the results were compare ... | 1986 | 3943276 |
| thymus independence of eosinophilia induced by a non-parasite antigen. | eosinophilia is primarily a t-cell-dependent phenomenon, related to helminthic infections. using a rat model (+/rnu), it has been shown that keyhole limpet hemocyanin (klh) in complete freund's adjuvant (cfa) induced blood eosinophilia, but only after pretreatment with cyclophosphamide (cy). cy alone caused an eosinophilopenia which was reversed by cfa or klh-cfa treatment. eosinophilia was also induced in congenitally athymic rnu/rnu rats after cy pretreatment and klh-cfa stimulation, the respo ... | 1986 | 3949415 |
| alkylating agents and mouse spermatogenesis: effects of a single dose of cyclophosphamide. | cyclophosphamide (cfa) is one of the alkylating agents which has now been used with some success in the treatment of human neoplasias and renal disease. to evaluate how this drug could affect the seminiferous epithelium, a single dose of cfa (200 mg/kg/weight) was given to twenty adult mice (strain a/sw). the effect of the drug was compared with a control group to which physiological saline solution was injected. mice were sacrificed by cervical dislocation at four different time intervals after ... | 1985 | 4025847 |