Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| brucellosis: an overview. | brucellosis remains a major zoonosis worldwide. although many countries have eradicated brucella abortus from cattle, in some areas brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. despite vaccination campaigns with the rev 1 strain, b. melitensis remains the principal cause of human brucellosis. brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. the recent isolation of distin ... | 1997 | 9204307 |
| infection of a pacemaker by brucella melitensis. | we report a case that, to the best of our knowledge, is the only published instance of infection of a pacemaker and its leads by brucella melitensis. furthermore, this case suggests that b. melitensis may be able to persist around pacemaker devices despite its having been eliminated from the rest of the body. the patient was a sheep shearer who had just undergone a 45-day course of antibiotic therapy for brucella and had been considered cured on the basis of negative blood cultures. | 1997 | 9205989 |
| [15th international symposium of salmonellosis and brucellosis: a report]. | 1997 | 9221550 | |
| [the functional metabolic activity of the blood neutrophils in different clinical forms of brucellosis]. | the characteristics of the activity of alkaline phosphatase, nonspecific esterase, myeloperoxidase, lysosomal cation proteins, lipids have been determined in the cytochemical study of neutrophils in peripheral blood samples of 127 patients with acute, chronic, primary chronic brucellosis and osteochondrosis. it is expedient to use these characteristics as additional tests for the differential diagnostics of different clinical forms of brucellosis, as well as residual brucellosis and osteochondro ... | 1997 | 9221662 |
| a review of the use of b. melitensis rev 1 vaccine in adult sheep and goats. | the live brucella melitensis rev 1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants. the classically recommended exclusive vaccination of young replacement animals has failed to control brucellosis in some developed countries and is frequently inapplicable in the developing world. accordingly, whole-flock vaccination is the only feasible alternative to control b. melitensis infection in small ruminants under the extensive management conditions ... | 1997 | 9234451 |
| serological findings obtained in cattle herds immunised with the brucella melitensis rev.1 and the b. abortus b19 vaccine in mongolia. | to reduce the economic losses caused by brucella abortus infection under the conditions of nomadic cattle breeding, more than half million cattle over 3 months of age (excluding male animals) were immunised with the b. melitensis rev.1 vaccine in 7 provinces of mongolia in the first year of a programme (1987). in the second year, only heifer calves over 3 months of age, yearling animals not vaccinated in the first year, as well as heifers over 2 years of age and cows were vaccinated. the vaccine ... | 1997 | 9270127 |
| identification of the major t-cell antigens present in the brucella melitensis b115 protein preparation, brucellergene ocb. | brucellergene is a commercial allergen prepared from brucella melitensis strain b115 and containing at least 20 cytoplasmic proteins. these proteins were separated by sds-page. the unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. the capacity of the separated proteins to elicit delayed-type hypersensitivity (dth) in infected guinea-pigs or to induce the production of interferon-gamma (ifn-gamma) by blood cells from infected cattle was evaluated. the bi ... | 1997 | 9291893 |
| localization and characterization of a specific linear epitope of the brucella dnak protein. | we have previously produced and characterized four monoclonal antibodies to the brucella dnak protein which were derived from mice infected with b. melitensis or immunized with the b. melitensis cell wall fraction. by use of a recombinant dna technique, we have localized a linear epitope, recognized by two of these monoclonal antibodies (v78/07b01/g11 and v78/09d04/d08), in the last 21 amino acids of the c-terminal region of the brucella dnak protein. the c-terminal region has been reported to b ... | 1997 | 9297829 |
| identification and characterization of brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. | in a previous report, proteins from brucella melitensis were characterized by two-dimensional polyacrylamide gel electrophoresis (2-d page) and n-terminal microsequencing. in the present report, we have extended this study to the second etiologic agent in ovine brucellosis, b. ovis, responsible for ram epididymitis and infertility. the combination of 2-d gel electrophoresis and protein microsequencing facilitated the location and identification of the major proteins of b. ovis on the 2-d pattern ... | 1997 | 9298663 |
| [fever, pulmonary infiltrates and hepatosplenomegaly]. | 1997 | 9304141 | |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| survival of a bacterioferritin deletion mutant of brucella melitensis 16m in human monocyte-derived macrophages. | a bacterioferritin (bfr) deletion mutant of brucella melitensis 16m was generated by gene replacement. the deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pbbr-bfr. the survival and growth of the mutant, b. melitensis pad 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (mdm). these results suggest that bfr is not essential for the intracellular survival of b. melitensis in human mdm. | 1997 | 9317046 |
| scrotal gray-scale and color doppler sonographic findings in genitourinary brucellosis. | brucellosis is an enzootic disease that is frequently transmitted from brucella-infected animals and their products to humans through the gastrointestinal tract. genitourinary complications are seen in 2-10% of cases. we used sonography to investigate such complications in patients from a brucella-endemic area. | 1997 | 9321717 |
| rapid detection of brucella melitensis from blood cultures by a commercial system. | to assess the capability of the peds plus medium of the bactec 9240 blood culture system to recover brucella melitensis within the routine seven-day protocol used by most clinical microbiology laboratories, inoculated blood culture bottles were monitored by the bactec 9240 instrument for four weeks, and blind subcultures were performed once a week. a total of 2579 blood cultures were drawn, 42 (1.6%) of which were positive for brucella melitensis. forty-one of the 42 (97.6%) positive cultures we ... | 1997 | 9323475 |
| [fever and dry cough in a construction worker from portugal]. | a 33-year-old portugese worker presented with a one-week history of nonproductive cough and fever. a presumptive diagnosis "viral infection of the respiratory tract" was made. however, because of persisting cough and fever further investigations were necessary, and finally brucella melitensis was isolated in blood cultures. three months before admission to the hospital the man was dressing the carcasses of a goat in portugal and consumpted fresh goats milk cheese. antibiotic therapy with rifampi ... | 1997 | 9340710 |
| brucellar osteomyelitis involving prosthetic extra-articular hardware. | we report the case of a patient with brucella melitensis osteomyelitis involving non-joint prosthetic implant of the femur. this is the first case published of osteomyelitis by brucella sp. in a patient with prosthetic implant in bone and the second one with both intra- or extra-articular prosthetic bone implant. brucella melitensis is a rare organism which causes osteomyelitis in patients with prosthetic hardware, and should be added to the list of suspected organisms responsible for this disea ... | 1997 | 9354359 |
| the differentiation of brucella species by substrate specific tetrazolium reduction. | in the development of a tetrazolium reduction assay to replace substrate stimulated oxygen uptake for the identification of brucella species, nine tetrazolium salts were evaluated. only the more readily reduced compounds (mtt and int) detected increased metabolic activity with the more fastidious, slow growing strains and with b. suis strains on l-arginine and dl-ornithine. the assay was optimised with mtt. mtt reduction profiles offered with the medium on which the cells were grown. cells grown ... | 1997 | 9355260 |
| a 74-year-old man with persistent fevers. | an elderly man with a history of extensive world travel presents with a chronic illness and fevers. the febrile illness has been present for eight years, and no diagnosis has been made despite extensive evaluation and testing. the differential diagnosis of this unusual case of fever of unknown origin is discussed. | 1997 | 9392941 |
| detection and differentiation of the six brucella species by polymerase chain reaction. | brucelosis is a severe acute febrile disease caused by bacteria of the genus brucella. its current diagnosis is based on clinical observations that may be complemented by serology and microbiological culture tests; however, the former is limited in sensitivity and specificity, the latter is time consuming. to improve brucelosis diagnosis we developed a test which is specific and sensitive and is capable of differentiating the six species of brucella. | 1997 | 9407549 |
| a brucella melitensis high-temperature-requirement a (htra) deletion mutant is attenuated in goats and protects against abortion. | it has been previously demonstrated that a brucella melitensis high-temperature-requirement a (htra) deletion mutant is more susceptible to oxidative killing in vitro than the parental strain and is attenuated in mice. to evaluate the contribution of the b melitensis htra protease to virulence in ruminants, the capacity of the b melitensis htra mutant rwp5 to produce abortion in goats was compared to that of the virulent parental strain 16m. experimental infection with strain 16m caused abortion ... | 1997 | 9429252 |
| [spontaneous bacterial peritonitis caused by brucella]. | 1997 | 9441188 | |
| the characterisation of brucella strains isolated from marine mammals. | small gram negative coccobacilli isolated from seals, porpoises, dolphins and from an otter road casualty were identified as brucellae by their colonial and cell morphology, staining characteristics, biochemical activity, agglutination by monospecific antisera and susceptibility to lysis by brucella specific bacteriophage. their characterisation, including metabolic profiles, is described. these strains could not be assigned to recognised nomen species of the genus brucella and it is suggested t ... | 1997 | 9444074 |
| evaluation of immunogenicity and protective activity in balb/c mice of the 25-kda major outer-membrane protein of brucella melitensis (omp25) expressed in escherichia coli. | the antibody response specific to the 25-kda major outer-membrane protein (omp25) of brucella melitensis expressed in escherichia coli was assessed in balb/c mice. groups of mice were immunised and boosted either with sonicated e. coli carrying plasmid pac2533-e. coli (pac2533)-expressing the gene coding for omp25 (omp25 gene) of b. melitensis, or with e. coli carrying plasmid puc19-e. coli (puc19). one control group received saline. the evolution of antibody responses was investigated by indire ... | 1998 | 9449948 |
| [aneurysm of the thoracic aorta and spondylodiscitis disclosing brucellosis]. | brucellosis, which decreased during the eighties in france, remains a public health problem in many mediterranean countries. we report the case of a 65-year old patient native of morocco, of thoracic aorta aneurysm and lumbar spondylodiscitis due to brucella melitensis, revealed by haemoptysis and lumbar pains, with a favourable outcome after aortic graft resection, spinal plaster immobilization and specific lengthy antibiotic treatment. this case report is characterized by the absence of endoca ... | 1997 | 9499988 |
| early clinical response to different therapeutic regimens for human brucellosis. | seventy-three cases of acute brucellosis were studied in relation to fever duration and hospital stay following different drug combinations, including gentamicin plus cotrimoxazole, rifampicin plus doxycycline, rifampicin plus cotrimoxazole, rifampicin plus tetracycline, streptomycin plus doxycycline, doxycycline plus cotrimoxazole, tetracycline plus cotrimoxazole, and tetracycline plus streptomycin. no statistical significant difference was found between these combinations regarding early clini ... | 1998 | 9502602 |
| prostatitis and hepatitis due to brucella melitensis: a case report. | a case is reported of a 43-year-old man who presented prostatitis and hepatitis due to brucella melitensis. his symptoms were icterus, weakness, anorexia, fever, and urinary discomfort. physical examination revealed icterus and hepatosplenomegaly. lymphomonocytosis, elevated erythrocyte sedimentation rate and abnormal liver functions had been detected in laboratory tests. brucella melitensis was isolated from prostatic fluid and blood cultures. | 1998 | 9515679 |
| human infection by brucella melitensis: an outbreak attributed to contact with infected goats. | although several outbreaks of brucella melitensis infection have been reported among laboratory workers or goat cheese consumers, outbreaks related to rural labour have been rarely studied. an outbreak of human brucellosis among farm workers of argentina was studied and revealed a close relationship with an epidemic of caprine abortions which occurred shortly before on the same farm. high rates of b. melitensis infection were found among goats. active brucellosis was diagnosed in 33 subjects (14 ... | 1997 | 9537757 |
| [the transmission of brucellosis via breast feeding. a report of 2 cases]. | 1998 | 9542227 | |
| characterization of an immuno-dominant antigen in brucella ovis and evaluation of its use in an enzyme-linked immunosorbent assay. | a panel of 45 brucella ovis serologically positive sera were tested in immunoblots against b. ovis outer membrane proteins omp31 and omp25, purified by preparative sds-gel electrophoresis. forty-three sera reacted with omp31, while only 11 reacted with omp25, suggesting that omp31 is identical to the previously reported immuno-dominant 29-kda protein. attempts to purify omp31 on a larger scale by using procedures such as ion exchange-, reversed phase-, affinity- and gel filtration chromatography ... | 1998 | 9549861 |
| brucella-infected ovarian dermoid cyst causing initial treatment failure in a patient with acute brucellosis. | a 37-year-old woman was diagnosed with acute brucellosis. despite appropriate antimicrobial therapy, high fever persisted. a contrast enhanced ct scan of the abdomen and pelvis revealed a left ovarian dermoid cyst. on the first postoperative day after left ovariectomy and right ovarian cystectomy were performed, the patient defervesced abruptly and continued to be completely afebrile. cultures from bone marrow and dermoid cyst grew brucella melitensis. the observation of a brucella-infected ovar ... | 1998 | 9561386 |
| [43-year-old patient with undulating fever of unknown origin and abdominal symptoms]. | 1998 | 9561451 | |
| isolation of brucella melitensis from alpine ibex. | eleven alpine ibex (capra ibex) and 27 chamois (rupicapra rupicapra) from gran paradiso national park (italy) were examined in march 1996. a 7-yr-old ibex buck had thick-walled carpal joints and enlargement of the right testicle characterized by necrosis and fibrosis. microscopically, testicular lesions were characterized by large areas of necrosis, fibrosis with irregular aggregates of macrophages and lymphocytes, and scattered foci of suppuration. specimens of the carpal bursae and testicle we ... | 1998 | 9577795 |
| identification, cloning and initial characterisation of feupq in brucella suis: a new sub-family of two-component regulatory systems. | to cause disease, brucella species have to adapt to a range of different environments. environmental sensing and adaptive responses in bacteria often involve the concerted action of a two-component regulatory system, consisting of sensor and response regulator components. amplification and sequence analysis of response regulators from brucella species identified a response regulator sequence with 96% similarity to rhizobium leguminosarum feup. in r. leguminosarum, the feupq two-component system ... | 1998 | 9595675 |
| evaluation of north american antibody detection tests for diagnosis of brucellosis in goats. | the sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. tests evaluated included the u.s. department of agriculture (usda) card test with 8% cell concentration (8%card), usda rapid automated presumptive test (rap), mexican rose bengal plate tests with 8 and 3% cell concentrations (8%rb and 3%rb), french rose bengal plate test with 4.5% cell concentration (4.5%rb), usda standard plate test (spt), usda buffered acidified plate agglutination test ... | 1998 | 9620406 |
| diarrhoea as the first manifestation of brucellosis. | 1998 | 9629985 | |
| central nervous system brucellosis: presentation, diagnosis and treatment. | although neurological symptoms in brucellosis are frequent, central nervous system (cns) involvement is uncommon. five patients with neurobrucellosis are presented. three patients presented with meningoencephalitis, one with polyradiculoneuritis and one with myelitis and an eighth nerve palsy. all patients had lymphocytic pleocytosis, low glucose and elevated levels of protein in the cerebrospinal fluid (csf). gamma-globulin and igg values in the csf were also increased. results of agglutination ... | 1998 | 9661940 |
| brucella melitensis infection in sheep: present and future. | sheep brucellosis, a zoonosis mainly due to b. melitensis (biovar 1, 2 or 3), remains widespread world-wide. pathologically and epidemiologically, the disease is very similar to b. abortus infection in cattle. the live b. melitensis rev 1 strain is currently considered as the best vaccine available for the control of sheep brucellosis, especially when used at the standard dose by the conjunctival route. used exhaustively in whole-flock vaccination programmes, it induces a great decrease in the p ... | 1998 | 9689741 |
| evaluation of a rough mutant of brucella melitensis in pregnant goats. | brucella melitensis strain vtrm1, a rough derivative of b melitensis strain 16m, is able to colonise the lymph nodes of goats, does not induce abortion in pregnant goats when used at doses leading to abortions with virulent strain 16m, and does not induce anti-o chain antibodies. however, strain vtrm1 as a single dose vaccine induces only partial protection against both infection and abortion following challenge. | 1998 | 9690613 |
| molecular cloning and characterization of cgs, the brucella abortus cyclic beta(1-2) glucan synthetase gene: genetic complementation of rhizobium meliloti ndvb and agrobacterium tumefaciens chvb mutants. | the animal pathogen brucella abortus contains a gene, cgs, that complemented a rhizobium meliloti nodule development (ndvb) mutant and an agrobacterium tumefaciens chromosomal virulence (chvb) mutant. the complemented strains recovered the synthesis of cyclic beta(1-2) glucan, motility, virulence in a. tumefaciens, and nitrogen fixation in r. meliloti; all traits were strictly associated with the presence of an active cyclic beta(1-2) glucan synthetase protein in the membranes. nucleotide sequen ... | 1998 | 9721274 |
| non-simultaneous brucella peritonitis and meningitis in a patient with a ventriculoperitoneal shunt. | 1998 | 9721968 | |
| paediatric neurobrucellosis: case report and literature review. | neurological complications are rare in childhood brucellosis: there are only 33 reported cases. in children, neurobrucellosis is usually of acute presentation involving the central nervous system. we report our experience with an 8-year-old boy with brucella meningitis who demonstrated a jarisch-herxheimer-like reaction, i.e. initial clinical deterioration following the commencement of antibrucella treatment, associated with increased pleocytosis and shift from lymphocytic to polymorphic predomi ... | 1998 | 9733381 |
| management of brucella endocarditis of a prosthetic valve. | a case of chronic brucella endocarditis of a prosthetic valve is reported. the diagnosis of this infection was established by positive blood cultures and high brucella agglutination titre. the patient was successfully managed by combination of medical therapy (consisting of streptomycin, trimethoprim-sulphamethoxazole, rifampin and tetracycline) and surgery. | 1998 | 9733385 |
| differentiation of brucella melitensis, b. ovis and b. suis biovar 2 strains by use of membrane protein- or cytoplasmic protein-specific gene probes. | the possibility of differentiating brucella species and biovars by southern blot hybridization of agarose gel-electrophoresed hindiii-digested genomic dna with membrane protein- or cytoplasmic protein-specific gene probes was investigated on 92 reference and field strains representative of all known species and biovars. based on the rflp pattern observed, three gene probes, i.e. br25, 39ugpa and omp16 coding for membrane or cytoplasmic proteins differentiated b. melitensis, b. ovis and b. suis b ... | 1998 | 9766202 |
| identification of brucella by ribosomal-spacer-region pcr and differentiation of brucella canis from other brucella spp. pathogenic for humans by carbohydrate profiles. | molecular and chemical characteristics often provide complementary information in the differentiation of closely related organisms. the genus brucella consists of a highly conserved group of organisms. identification of the four species pathogenic in humans (brucella melitensis, brucella abortus, brucella suis, and brucella canis) is problematic for many clinical laboratories that depend primarily on serology and phenotypic characteristics to differentiate species. pcr amplification of the 16s-2 ... | 1998 | 9774568 |
| identification of the perosamine synthetase gene of brucella melitensis 16m and involvement of lipopolysaccharide o side chain in brucella survival in mice and in macrophages. | brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. the smooth lipopolysaccharide (s-lps) has been reported to be an important virulence factor of these organisms, but the genetic basis of expression of the s-lps o antigen has not yet been described. likewise, the role of the o side chain of s-lps in the survival of brucella has not been clearly defined. a mini-tn5 transposon mutant library of brucella melitensis 16m was screened b ... | 1998 | 9784561 |
| effect of p39 gene deletion in live brucella vaccine strains on residual virulence and protective activity in mice. | the 39-kilodalton protein (p39) has previously been shown to be an immunodominant protein in brucella infections. p39 gene deletion mutants of vaccine strains brucella abortus s19 and brucella melitensis rev.1 were constructed by gene replacement. this deletion did not significantly modify the residual virulence of both vaccine strains in cd-1 mice. cd-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. mutant vaccine strains devoid of p39 could provi ... | 1998 | 9784574 |
| evaluation of lipopolysaccharides and polysaccharides of different epitopic structures in the indirect enzyme-linked immunosorbent assay for diagnosis of brucellosis in small ruminants and cattle. | brucella abortus and brucella melitensis have surface lipopolysaccharides and polysaccharides carrying b. melitensis-type (m) and b. abortus-type (a) epitopes as well as common (c) epitopes present in all smooth brucella biotypes. crude lipopolysaccharides, hydrolytic o polysaccharides, and native hapten polysaccharides of mc or ac specificity were evaluated in indirect enzyme-linked immunosorbent assays with polyclonal, monoclonal, or protein g conjugates by using sera from cattle, sheep, and g ... | 1998 | 9801329 |
| comparison of polyclonal, monoclonal and protein g peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis in sheep. | the sensitivities of three commercial peroxidase conjugates (polyclonal anti-sheep igg, recombinant protein g and a monoclonal anti-ruminant igg1) in an enzyme-linked immunosorbent assay for brucella ovis were evaluated. the monoclonal and protein g conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella-free sheep. the protein g conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion ... | 1998 | 9802197 |
| [epidemiological analysis on the rising of brucellosis prevalence in the area already under controlled]. | an epidemiological analysis was made on the outbreak of brucellosis in sui-de county, shaanxi province in 1995-1996. result showed that it was one of the most serious brucellosis outbreaks since 1986 when national brucellosis under controlled critiria had been met in the province, and the number of cases was continuously spreading and growing. the incidence was up to 84.8 per 10(5) and the prevalence rate reached 17.5 percent. patients had typical symptoms with bad conditions. most all the cases ... | 1997 | 9812474 |
| antibrucella activity of quinolone sparfloxacin. | background: sparfloxacin is a new fluoroquinolone with excellent in vitro activity against a variety of gram-positive and gram-negative bacteria. methods: in vitro antibacterial activity of this new drug was tested against 139 strains of brucella melitensis. these strains were isolated from 138 patients at a major tertiary care referral center in riyadh, saudi arabia. results: seven percent of the strains were inhibited by 0.06 mg per l and 99% by 0.12 mg per l of sparfloxacin. of the five other ... | 1995 | 9815397 |
| in vitro activities of six new fluoroquinolones against brucella melitensis. | we have tested the in vitro activities of eight fluoroquinolones against 160 brucella melitensis strains. the most active was sitafloxacin (mic at which 90% of the isolates are inhibited [mic90], 0.12 microg/ml). in decreasing order, the activities (mic90s) of the rest of the tested fluoroquinolones were as follows: levofloxacin, 0.5 microg/ml; ciprofloxacin, trovafloxacin, and moxifloxacin, 1 microg/ml; and ofloxacin, grepafloxacin, and gatifloxacin, 2 microg/ml. | 1999 | 9869594 |
| efficacy of elisa compared to conventional tests (rbpt and cft) for the diagnosis of brucella melitensis infection in sheep. | an indirect elisa (ielisa) using a b. abortus smooth lipopolysaccharide (s-lps) as coating antigen and a polyclonal anti-sheep igg peroxydase-labeled serum as conjugate was developed. the ielisa was assessed in comparison to the food and agriculture organisation/international atomic energy agency (fao/iaea) standard bovine elisa kit (iaea kit) using an anti-bovine igg conjugate that cross-reacts with sheep antibodies, and with complement fixation test (cft) and rose bengal plate test (rbpt). the ... | 1998 | 9874104 |
| risk factors for brucellosis seropositivity of goat herds in the mexicali valley of baja california, mexico. | a case-control study was conducted in the mexicali valley to identify risk factors for goat-herd seropositivity for brucella melitensis. nineteen case herds (> or = 2 positive results with the 8% rose bengal plate test (rbt)) and 55 control herds (zero positive results in rbt), matched for herdsize and geographic location, were enrolled. conditional logistic regression was used to construct a multivariable model of the odds of seropositivity using variables assessed in a questionnaire administer ... | 1998 | 9879591 |
| enzyme-linked immunosorbent assay for brucella antigen detection in human sera. | a sandwich enzyme-linked immunosorbent assay (elisa) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples. under optimum conditions, 100 brucella cells/well or 10(5) fg/ml of lipopolysaccharide (lps) were detected in spiked specimens. the standardised assay was performed on 1607 sera from random blood donors, 146 patients with brucellosis, 20 persons in high risk groups and 264 sera from patients with diseases other than brucell ... | 1998 | 9879960 |
| quantitative assessment by flow cytometry of t-lymphocytes producing antigen-specific gamma-interferon in brucella immune cattle. | peripheral blood mononuclear cells (pbmc) isolated from cattle infected with brucella secreted gamma-interferon (ifn-gamma) after antigen-specific stimulation with brucellergene, which is a mixture of cytoplasmic proteins of rough brucella melitensis b115. following the depletion of the monocyte-macrophages from the pbmc, the enriched lymphocyte populations stimulated with brucellergene did not produce ifn-gamma. two-colour immunofluorescence staining of intracellular ifn-gamma and bovine cell s ... | 1998 | 9880107 |
| leukocytoclastic vasculitis as a cutaneous manifestation of systemic infection caused by brucella melitensis. | a 22-year-old man with leukocytoclastic vasculitis as a cutaneous manifestation of brucellosis is presented. skin lesions resolved 48 hours after starting treatment with tetracycline and rifampicin. although skin manifestations are rarely associated with infection due to brucella and do not seem to worsen the prognosis, they may cause the patient to seek medical attention as well as contribute to the diagnosis. | 1999 | 9951590 |
| immunogenicity of recombinant escherichia coli expressing the omp31 gene of brucella melitensis in balb/c mice. | balb/c mice were immunized with recombinant escherichia coli expressing the omp31 gene of brucella melitensis, a gene coding for a major outer membrane protein. immunization resulted in the production of specific antibodies to b. melitensis in the serum, the production of which was considerably increased after boosting with a dose ten times lower than the first. a significant specific proliferative response of immune spleen cells to b. melitensis was observed 5 weeks after the first immunization ... | 1999 | 9987174 |
| differentiation of microorganisms based on pyrolysis-ion trap mass spectrometry using chemical ionization. | the ability to differentiate microorganisms using pyrolysision trap mass spectrometry was demonstrated for five gram-negative disease-causing organisms: brucella melitensis, brucella suis, vibrio cholera, yersinia pestis, and francisella tularensis. bacterial profiles were generated for gamma-irradiated bacterial samples using pyrolytic methylation and compared for electron ionization and chemical ionization using several liquid reagents with increasing proton affinities. electron ionization com ... | 1999 | 9989380 |
| performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis. | competitive and standard enzyme-linked immunosorbent assays (elisas), rose bengal (rb), complement fixation, and agar gel immunoprecipitation with native hapten (agid-nh) were compared by using sera from brucella-free, brucella melitensis-infected, and b. melitensis rev1-vaccinated sheep. the most sensitive tests were indirect elisa and rb, and the most specific tests were agid-nh and competitive elisa. we show that rb followed by agid-nh is a simple and effective system for diagnosing sheep bru ... | 1999 | 10066666 |
| brucella melitensis 16m: characterisation of the gale gene and mouse immunisation studies with a gale deficient mutant. | the gale gene of streptomyces lividans was used to probe a cosmid library harbouring brucella melitensis 16m dna and the nucleotide sequence of a 2.5 kb clai fragment which hybridised was determined. an open reading frame encoding a predicted polypeptide with significant homology to udp-galactose-4-epimerases of brucella arbortus strain 2308 and other bacterial species was identified. dna sequences flanking the b. melitensis gale gene shared no identity with other gal genes and, as for b. abortu ... | 1999 | 10068125 |
| [development of a method for alkaline extraction of dna from brucella for diagnosing brucellosis using the polymerase chain reaction]. | several methods of alkaline extraction of chromosome dna from brucella in the presence of 50 microliters model diagnostic material blood serum are developed for the diagnosis of brucellosis by the polymerase chain reaction (pcr). these methods are based on the capacity of naoh to effectively denature proteins and destroy brucella cell wall, thus isolating the genome dna without exposure to proteolytic enzymes, detergents, deproteinization, or ph neutralization. the first method consists in alkal ... | 1999 | 10190109 |
| in vitro activities of antibiotics alone and in combination against brucella melitensis at neutral and acidic phs. | brucellae survive acidic phs in phagolysosomes. azithromycin, streptomycin, and quinolones were active against brucella melitensis at ph 7.0 but not at ph 5.0; rifampin and doxycycline retained activity at ph 5.0. regardless of ph, azithromycin-rifampin and ofloxacin-rifampin showed less synergy than established streptomycin-doxycycline and rifampin-doxycycline combinations. | 1999 | 10223958 |
| effect of early antibiotic treatment on the antibody response to cytoplasmic proteins of brucella melitensis in mice. | to test whether antibiotic therapy hampers the antibody response to brucella antigens, 30 balb/c mice were infected with brucella melitensis h38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups dox7 and dox28, respectively) or for no treatment (control group). antibodies to smooth lipopolysaccharide (lps) reached peak levels (mean optical density [od] = 2.618) between days 56 and 70 p.i. in the control ... | 1999 | 10225853 |
| molecular characterization of a brucella species large dna fragment deleted in brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. | a brucella melitensis 16m dna fragment of 17,119 bp, which contains a large region deleted in b. abortus strains and dna flanking one side of the deletion, has been characterized. in addition to the previously identified omp31 gene, 14 hypothetical genes have been identified in the b. melitensis fragment, most of them showing homology to genes involved in the synthesis of a polysaccharide. considering that 10 of the 15 genes are missing in b. abortus and that all the polysaccharides described in ... | 1999 | 10338472 |
| [neurological manifestations indicative of brucellosis]. | eight patients presented neurological signs secondary to brucella infection. the clinical presentation was a meningoencephalitis in three cases, a meningoencephalomyelitis in one case, an epiduritis with spinal cord compression in one case, an acute polyradiculoneuritis in two cases and a chronic polyradiculoneuritis in one case. acoustic nerve was impaired in seven cases. cerebrospinal fluid (csf) analysis revealed a lymphocytic meningitis and a high protein concentration in all cases. the aggl ... | 1999 | 10339791 |
| time for a fresh look at the bacterial chromosome. | 1999 | 10366858 | |
| investigation into the role of the response regulator ntrc in the metabolism and virulence of brucella suis. | during infection, brucella species have to adapt to a range of different environments. environmental sensing in bacteria often involves the concerted action of two-component regulatory systems consisting of sensor and response regulator components. in this study, we identified, cloned and sequenced four independent response regulator gene fragments from brucella melitensis. one amplified gene fragment showed nearly 90% identity to the response regulator subfamily of ntrc transcriptional activato ... | 1999 | 10373105 |
| increasing resistance of brucellae to co-trimoxazole. | 1999 | 10383374 | |
| an adolescent pakistani girl with chronic meningitis. | 1999 | 10396591 | |
| who consultation on the development of new/improved brucellosis vaccines. 17 december 1997, geneva, switzerland. | 1998 | 10403040 | |
| bilateral dacryoadenitis associated with brucellosis. | 1999 | 10406164 | |
| in vitro activities of five new antimicrobial agents against brucella melitensis. | 1999 | 10418765 | |
| the effects of electron and chemical ionization modes on the ms profiling of whole bacteria. | free fatty acid profiling of whole bacteria [francisella tularensis, brucella melitensis, yersinia pestis, bacillus anthracis (vegetative and sporulated), and bacillus cereus] was carried out with direct probe mass spectrometry under 70-ev electron ionization (ei) and isobutane chemical ionization in both the positive (ci+) and negative modes (ci-). electron ionization produced spectra that contained molecular ions and fragment ions from various free fatty acids. spectra acquired with isobutane ... | 1999 | 10439512 |
| use of mass vaccination with a reduced dose of rev 1 vaccine for brucella melitensis control in a population of small ruminants. | mass vaccination with reduced dose 1/50 rev 1 strain live vaccine (1-2 10(9) colony forming units), administered subcutaneously, over a four and a half year period reduced the prevalence of brucella melitensis in kuwait's small ruminant population from 5.8% in 1993 to 2.02% in 1997. serological test results using the rose bengal plate test, rivanol agglutination test and complement fixation showed no evidence of persistence of positive serology in animals nine or more months after vaccination. q ... | 1999 | 10445249 |
| outer membrane proteins omp10, omp16, and omp19 of brucella spp. are lipoproteins. | the deduced sequences of the omp10, omp16, and omp19 outer membrane proteins of brucella spp. contain a potential bacterial lipoprotein processing sequence. after extraction with triton x-114, these three proteins partitioned into the detergent phase. processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. the three proteins were radioimmunoprecipitated from [(3)h]palmitic acid-labeled brucella abortus lysates with monoclonal antibodies. ... | 1999 | 10456959 |
| vertebral osteomyelitis in northern spain. report of 62 cases. | the records of 62 patients with clinical and radiographic evidence of vertebral osteomyelitis and positive bacteriological diagnosis, seen between 1979 and 1996, were reviewed in order to gather data on the epidemiology and the clinical pattern displayed by patients with this condition in northern spain. | 1999 | 10464555 |
| identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains. | brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ... | 1999 | 10473532 |
| bilateral mammary abscess due to brucella melitensis. | brucellosis is an important zoonosis, which is widespread as endemic cases in turkey. unusual clinical features and complications can sometimes lead to diagnostic difficulties. we describe here a case of a pregnant woman with bilateral mammary abscesses due to brucella melitensis. | 1999 | 10482066 |
| spontaneous bacterial peritonitis due to brucella melitensis in a cirrhotic patient. | 1999 | 10484037 | |
| brucella spp. peritonitis. | 1999 | 10492334 | |
| divergence paralysis & intracranial hypertension due to neurobrucellosis. a case report. | case report: a 22 year old female presented with sudden onset of uncrossed diplopia at distance, intracranial hypertension, esotropia and was evaluated. microbiological tests of csf and sera showed for brucellosis and the patient received therapy for this and her intracranial hypertension. the papilledema, headache, esotropia and diplopia all disappeared after therapy. conclusions: diagnostic tests for brucella must be considered for patients who have divergence palsy and papilledema, especially ... | 1999 | 10506689 |
| [brucella melitensis biovar 3 in argentina?]. | 1999 | 10509416 | |
| acute calcular cholecystitis in a patient with brucellosis. | 1999 | 10517200 | |
| protection of mice against brucellosis by vaccination with brucella melitensis wr201(16mdeltapurek). | human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. a vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. vaccines currently used in animals are unsuitable for human use. we tested a live, attenuated, purine-auxotrophic mutant strain of brucella melitensis ... | 1999 | 10531243 |
| supramolecular organization of photosystem ii and its light-harvesting antenna in partially solubilized photosystem ii membranes. | we present an extended analysis of the organization of green plant photosystem ii and its associated light-harvesting antenna using electron microscopy and image analysis. the analysis is based on a large dataset of 16 600 projections of negatively stained psii-lhcii supercomplexes and megacomplexes prepared by means of three different pretreatments. in addition to our previous work on this system [boekema, e.j., van roon, h., calkoen, f., bassi, r. and dekker, j.p. (1999) biochemistry 38, 2233- ... | 1999 | 10561584 |
| brucellosis outbreak: analysis of risk factors and serologic screening. | israel is one of the mediterranean countries in which brucellosis is endemic. as recently there has been a brucellosis outbreak in a kibbutz, the aim of this study is to identify asymptomatic infected kibbutz members, and to delineate the manner of infection in this setting. therefore, all the asymptomatic kibbutz members were screened by the rose bengal test for brucellosis, while both patients and healthy members were asked to fill in a questionnaire in order to pinpoint the manner of infectio ... | 1999 | 10567679 |
| [acute cholecystitis during systemic brucellosis]. | 1999 | 10568164 | |
| comparison of e test to microdilution for determining in vitro activities of antibiotics against brucella melitensis. | 1999 | 10577353 | |
| brucellar spondylitis: review of 35 cases and literature survey. | thirty-five patients aged 14-74 years (average, 54 years) who had brucellar spondylitis were treated between january 1991 and december 1997. the time from onset of symptoms to diagnosis of spondylitis ranged from 1 week to 8 months (median, 9 weeks). back or neck pain (100% of patients), fever (66%), and constitutional symptoms (57%) were the most common symptoms. cultures of blood specimens from 26 patients (74%) were positive for brucella melitensis. the duration of antimicrobial therapy (medi ... | 1999 | 10585793 |
| epidemiology and control of brucellosis in ruminants from 1986 to 1996 in malta. | the epidemiology and control of brucella melitensis in malta was analysed using herd test data made available by the veterinary service of malta. the eradication scheme commenced in 1987 with the introduction of a test and slaughter scheme using the rose bengal test. herds registered with malta dairy products limited (mdp) showed a herd prevalence of 23% in 1987 which fell to less than 1.5% by 1993. prevalence rose to 13% in 1995. herds not delivering milk to the mdp showed an initial herd preva ... | 1999 | 10588008 |
| cold agglutinins as a fast clue to brucellosis? | 1999 | 10593136 | |
| effects of opsonization and gamma interferon on growth of brucella melitensis 16m in mouse peritoneal macrophages in vitro. | entry of opsonized pathogens into phagocytes may benefit or, paradoxically, harm the host. opsonization may trigger antimicrobial mechanisms such as reactive oxygen or nitric oxide (no) production but may also provide a safe haven for intracellular replication. brucellae are natural intramacrophage pathogens of rodents, ruminants, dogs, marine mammals, and humans. we evaluated the role of opsonins in brucella-macrophage interactions by challenging cultured murine peritoneal macrophages with bruc ... | 2000 | 10603396 |
| [liver abscess probably caused by brucella]. | 1999 | 10614086 | |
| chronic intracerebral brucella abscess. case illustration. | 2000 | 10616102 | |
| [increased production of the protein antigen of brucella melitensis in escherichia coli k-12 cells]. | the production of brucella melitensis protein antigen with molecular weight of 38 kd in escherichia coli k-12 cell lysates has been studied by immunoblotting with various antisera. e. coli strains differed by the vector plasmid and the size of b. melitensis 565 dna fragment with 38 kd protein gene, cloned in this plasmid. the immunoblotting analysis detected increased production of 38 kd protein in the recombinant gse830 strain in comparison with the b. melitensis strain 565, from which the gene ... | 1999 | 10621933 |
| iliopsoas bursitis due to brucella melitensis infection--a case report. | 1999 | 10622492 | |
| lipopolysaccharides of brucella abortus and brucella melitensis induce nitric oxide synthesis in rat peritoneal macrophages. | smooth lipopolysaccharide (s-lps) and lipid a of brucella abortus and brucella melitensis induced the production of nitric oxide (no) by rat adherent peritoneal cells, but they induced lower levels of production of no than escherichia coli lps. the participation of the inducible isoform of no synthase (inos) was confirmed by the finding of an increased expression of both inos mrna and inos protein. these observations might help to explain (i) the acute outcome of brucella infection in rodents, ( ... | 2000 | 10679001 |
| brucellosis in a mother and her young infant: probable transmission by breast milk. | brucellosis, although primarily a zoonotic infection, is also a threat for human health. infection can be transmitted to humans through direct contact with infected animals, products of conception, or animal discharges, and through consumption of potentially infected milk, milk products, or meat. human-to-human transmission is rare. there have been case reports of transmission via blood transfusion and bone marrow transplantation from infected donors. sexual intercourse is a possible means of tr ... | 2000 | 10689217 |
| topology prediction of brucella abortus omp2b and omp2a porins after critical assessment of transmembrane beta strands prediction by several secondary structure prediction methods. | in order to propose a reliable model for brucella porin topology, several structure prediction methods were evaluated in their ability to predict porin topology. four porins of known structure were selected as test-cases and their secondary structure delineated. the specificity and sensitivity of 11 methods were separately evaluated. our critical assessment shows that some secondary structure prediction methods (phd, dsc, sopma) originally designed to predict globular protein structure are usefu ... | 2000 | 10698111 |
| molecular characterization of brucella strains isolated from marine mammals. | recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as brucella species by conventional phenotypic analysis. this study found the 16s rrna gene from one representative isolate was identical to the homologous sequences of brucella abortus, b. melitensis, b. canis, and b. suis. is711-based dna fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical brucella species. in general, fingerprint patterns grouped by ... | 2000 | 10699036 |