Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a selectable temperature-sensitive v-src moloney retrovirus. | we have cloned the v-src gene of ts339b77 rsv into a new moloney-based retroviral expression vector (yn). in the resulting construct (ts339ynsrc), the ts339 gene is transcribed from the viral ltr, while a selectable resitance marker, the tn5 neomycin phosphotransferase (neor) gene, is transcribed from an internal thymidine kinase (tk) promoter. g418 resistance and focus formation were induced in nih3t3 cells at comparable efficiencies within the permissive temperature range (33-37 degrees c). at ... | 1988 | 2485257 |
| detection of bovine respiratory syncytial virus using a heterologous antigen-capture enzyme immunoassay. | based on the marked antigenic similarities that exist between antigens of the human and bovine strains of respiratory syncytial virus (rsv), an enzyme immunoassay (eia) designed to detect human rsv was used to detect bovine rsv. the commercial test kit (rsv eia) consists of a solid phase (beads) coated with a capture antiserum prepared against the long strain of human rsv. the rsv eia test was compared with the method of inoculation of cell cultures and fluorescent antibody (fa) staining of lung ... | 1989 | 2488346 |
| nosocomial viral infections: recent developments and new strategies. | surveillance studies generally have underestimated the importance of viruses in the pathogenesis of nosocomial respiratory tract infections. the development of rapid, sensitive and reliable diagnostic techniques has permitted documentation of viral respiratory pathogens and has led to increased understanding of the epidemiology of these organisms in the hospital. these advances are exemplified by studies of respiratory syncytial virus infections in hospitalized children. the pulmonary sequelae o ... | 1989 | 2495955 |
| immunization of the gastrointestinal tract with bacterial and viral antigens: implications in mucosal immunity. | the effects of oral immunization with pseudomonas aeruginosa (paoi), chlamydia trachomatis or respiratory syncytial virus (rsv) on the development of specific antibody responses in the intestine, respiratory tract and genital secretions was studied in several animal models. oral immunization resulted in the development of specific immunity in distant mucosal sites. however, its role in influencing the outcome of reinfection challenge at the distant site varied with the antigen. little or no prot ... | 1989 | 2499537 |
| [clinical studies on the secondary bacterial infection in respiratory syncytial virus infection of children]. | secondary bacterial infection was studied on 231 children admitted with respiratory syncytial virus (rsv) infection in the 10 years since 1987. of the 231 children, 56 (24.2%) had dual bacterial infection possibly due to secondary bacterial invasion. the diagnoses of bacterial disease were sepsis (2), pyothorax (2), pneumonias (41), otitis media (7), nasopharyngitis (2) and urinary tract infection (2). dual bacterial infections were more frequent in infants and children over 6 months than in inf ... | 1989 | 2504838 |
| [induction of unstable mutations in drosophila melanogaster by microinjections of dna from oncogene viruses into the embryonic polar plasm. part iv. malignantization of tissues of mutant larvae]. | we have demonstrated that larval tissues of mutant stocks induced by injections of oncogene virus dna (sa7 and rsv) show the neoplastic mode of growth after transplantation into the body cavity of wild-type flies. neoplasms tested were shown to be ordinary benign insect neoplasms, and at the same time they show the dominant mode of heredity typical of the neoplasms of vertebrates. genetic factors responsible for the neoplastic growth are localised in the 3rd chromosome in the stocks obtained aft ... | 1989 | 2509289 |
| an experimental approach to specific adoptive immunotherapy for malignant brain tumors. | with the aid of interleukin 2 (il-2), two phenotypically different cytotoxic t lymphocyte (ctl) clones were established with target specificity against syngeneic murine malignant brain tumor (a methylcholanthrene-induce ependymoblastoma of c57bl/6 mouse origin, 203-glioma). furthermore, the cloned ctl lines were characterized in vitro, and their in vivo effectiveness was investigated by intracerebral (i.c.) tumor neutralization assay and adoptive immunotherapy with the clones for i.c. tumor-bear ... | 1989 | 2519184 |
| the 1b (ns2), 1c (ns1) and n proteins of human respiratory syncytial virus (rsv) of antigenic subgroups a and b: sequence conservation and divergence within rsv genomic rna. | a 2330 nucleotide sequence spanning the 1b (ns2), ic (ns1) and n genes and intergenic regions of human respiratory syncytial virus strain 18537, representing antigenic subgroup b, was determined by sequencing cloned cdnas of intracellular mrnas. comparison with the previously reported sequences for strain a2 of subgroup a showed that 1b, 1c and n were highly conserved at the nucleotide level (78, 78 and 86% identity, respectively) and at the amino acid level (92, 87 and 96% identity, respectivel ... | 1989 | 2525176 |
| in vitro performance of the small particle aerosol generator (spag-2). | the anti-viral drug ribavirin primarily used for the treatment of respiratory syncytial virus (rsv) infection in infants is delivered by continuous nebulization with the small particle aerosol generator (spag). clinical data suggest that the spag is an efficient nebulizer for ribavirin; it is also being used for the delivery of other pulmonary agents. the efficiency of a nebulizer can be defined in large part by delivering a major proportion of its output with a particle size of 1 to 5 microns w ... | 1989 | 2529473 |
| t-cells in respiratory syncytial virus (rsv) infection. | 1989 | 2533461 | |
| a rat mutant cell clone showing temperature-dependent transformed phenotypes with functional expression of the src gene product. | the cellular mutant b814 isolated from a fischer rat cell line shows temperature-sensitivity of focus formation on infection with moloney murine sarcoma virus (mo-msv) and rous sarcoma virus (rsv). an rsv-transformed clone (s814-2) isolated from b814 cells shows temperature-sensitive transformed phenotypes for morphology, growth in soft agar, and glucose uptake. the expression, phosphorylation, and tyrosine kinase activity of pp60v-src in s814-2 were not affected at the nonpermissive temperature ... | 1989 | 2535907 |
| ability of mammalian fibroblasts to grow in synthetic medium containing neither serum nor exogenously added macromolecules. | rous sarcoma virus transformed chinese hamster fibroblasts, clone chr1-3, were established at high temperature, then subcloned. six subclones with round and flat morphology harboring undeleted and partially deleted rsv proviruses, respectively, were seeded into serum-free synthetic medium with no macromolecular additives, and maintained for 2 mo. one flat subclone no. 14, fully designated sfchr1-3.14 for its serum-free phenotype, was further propagated in the same medium. the cells grew exponent ... | 1989 | 2536651 |
| a method for infection of cultured myogenic cells with rous sarcoma virus using polybrene. | a method for efficiently infecting primary myogenic cultures with a temperature-sensitive variant of the prague strain of rous sarcoma virus (rsv, tsla24) to obtain a high yield of transformed myogenic cells is reported. it incorporates the use of an amorphous polymer of polycations, polybrene, to enhance the absorption of the virus by the muscle cells. in addition, other steps which were shown to be important were a) to allow cell attachment before infection, b) to infect at 35 degrees c in low ... | 1989 | 2536652 |
| crystal structure of a retroviral protease proves relationship to aspartic protease family. | retroviral gag, pol and env gene products are translated as precursor polyproteins, which are cleaved by virus-encoded proteases to produce the mature proteins found in virions. on the basis of the conserved asp-thr/ser-gly sequence at the putative protease active sites, and other biochemical evidence, retroviral proteases have been predicted to be in the family of pepsin-like aspartic proteases. it has been suggested that aspartic proteases evolved from a smaller, dimeric ancestral protein, and ... | 1989 | 2536902 |
| comparison of the regional distribution of calspectin (nonerythroid spectrin or fodrin), alpha-actinin, vinculin nonerythroid protein 4.1, and calpactin in normal and avian sarcoma virus- or rous sarcoma virus-induced transformed cells. | with fluorescence and interference reflection microscopy (irm), we compared the regional distribution of calspectin, its interacting proteins (nonerythroid protein 4.1 and calpactin), alpha-actinin, and vinculin in nrk cells and their avian sarcoma virus (asv)- or temperature-sensitive (ts) rous sarcoma virus (rsv)-transformed cells. the localization of these cytoskeletal proteins was determined with the specific antibodies. in nrk cells, alpha-actinin and vinculin were concentrated at adhesion ... | 1989 | 2537219 |
| dissociation of inositol trisphosphate from diacylglycerol production in rous sarcoma virus-transformed fibroblasts. | the metabolism of phosphatidylinositol (pi) and related intermediates was studied in uninfected and rous sarcoma virus-(rsv) infected chicken embryo fibroblasts (cefs). cells infected with wild-type rsv exhibited twofold increases in steady-state concentrations of inositol trisphosphate (ip3) and inositol bisphosphate (ip2) as compared to uninfected cefs. in addition, increased concentrations of ip3 and ip2 were observed in cefs infected with the rsv temperature-sensitive transformation mutant n ... | 1989 | 2537320 |
| identification of a phorbol ester-repressible v-src-inducible gene. | chicken embryo fibroblasts (cef) infected with a temperature-sensitive rous sarcoma virus (rsv) mutant, tsny72-4, express a set of pp60v-src-induced rnas soon after shift to the permissive temperature. by subtractive and differential screening, we have cloned 12 of these sequences, 2 of which were c-fos and krox-24. serum induced all the v-src-inducible genes tested, suggesting that these genes serve roles in normal cell division and are not specific to transformation per se. significantly, howe ... | 1989 | 2537491 |
| restricted clonality of visceral sarcomas in avian sarcoma virus-infected chickens. | experiments were undertaken to analyze proviral dna in primary (wing web) and visceral sarcomas arising in fp chickens infected with bh-rsv(rav-2). using the degree of heterogeneity of bh-rsv proviral integration sites as a measure of the degree of polyclonality of sarcoma tissue, we observed that a high proportion of the visceral sarcomas examined comprised dominant clones, independently of whether these sarcomas were isolated from immune-suppressed or nonsuppressed infected chickens; by contra ... | 1989 | 2538025 |
| response of major histocompatibility (b) complex haplotypes b22, b26, and b30 to rous sarcomas. | the response to rous sarcoma virus-induced tumors was measured over a 2-yr period using line unh 105 new hampshire chickens segregating for three major histocompatibility (b) complex haplotypes, b22, b26, and b30. twelve sires and 49 dams were used to produce 489 pedigreed progeny consisting of six b genotypes b22/b22, b22/b26, b22/b30, b26/b26, b26/b30, and b30/b30. six-wk-old chicks were inoculated in the wingweb with a pseudotype of bryan high-titer rous sarcoma virus designated as bh rsv (ra ... | 1989 | 2539589 |
| expression of a novel gene encoding a 51.5 kd precursor protein is induced by different retroviral oncogenes in quail neuroretinal cells. | a cdna clone, named t64, was isolated from a library of quail neuroretinal cells transformed by a thermosensitive v-src mutant of rous sarcoma virus. it corresponds to the most abundant mrna with thermodependent expression in these cells. t64 accumulation also correlated with pp60v-src activity in other cell types transformed by rsv, such as fibroblasts and myoblasts, but was independent of the proliferative state of the cells, indicating that t64 is rather implicated in the process of morpholog ... | 1989 | 2541393 |
| hiv-1 expression is posttranscriptionally repressed in drosophila cells. | the long terminal repeats (ltrs) of the human immunodeficiency virus (hiv) the rous sarcoma virus (rsv) and the copia drosophila retrotransposon were compared in their capacity to direct expression of the bacterial cat (chloramphenicol acetyltransferase) gene in human, murine, and drosophila cell lines. the results indicate that hiv and rsv ltr expression is post transcriptionally repressed in the drosophila cells while copia ltr expression is post-transcriptionally repressed in the human and mu ... | 1989 | 2541751 |
| efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle. | efficacy and safety of components of an im-administered vaccine for prevention of infectious bovine rhinotracheitis virus (ibrv), parainfluenza type-3 (pi-3) virus, bovine viral diarrhea virus (bvdv), and respiratory syncytial virus (rsv) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. challenge of immunity tests were conducted in calves for ibrv, pi-3 virus, or bvdv vaccinal components. all inoculated calves developed serum-neutr ... | 1989 | 2542202 |
| suppression of rous sarcoma virus-induced tumor formation by preinfection with viruses encoding src protein with novel n termini. | two recovered avian sarcoma viruses (rasvs), rasv157 and rasv1702, encode src products which contain novel, nonmyristoylated n-terminal amino acids. these viruses transform chicken embryo fibroblasts and cause tumors in chicks. however, the tumors rasvs induce are small and regress within 2 weeks. to determine whether this regression results from weak tumorigenicity or from the active immunity of the host, we injected 1-week-old chicks with rasv and several days later injected the chicks with ch ... | 1989 | 2542564 |
| clinical manifestations of respiratory tract infections due to respiratory syncytial virus and rhinoviruses in hospitalized children. | from september 1984 to may 1986, nasopharyngeal secretions were obtained from 519 children with some form of respiratory tract infection. the nasal secretions were screened for respiratory syncytial virus (rsv), rhinoviruses, adenoviruses, parainfluenza virus types 1, 2, 3, influenza virus types a and b, and enteroviruses by tissue culture virus isolation technique and/or enzyme-linked immunosorbent assay. a uniform questionnaire gave information about age, sex, individual signs and symptoms, fi ... | 1989 | 2545074 |
| comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections. | a comparison of immunofluorescence (if), enzyme-linked immunosorbent assay (elisa), and isolation in tissue culture (tc) for detection of respiratory viruses was performed on 496 nasopharyngeal aspirates from children under 5 years of age with lower acute respiratory infections who were receiving attention at three hospitals in buenos aires, argentina. all samples were tested by the three methods for respiratory syncytial virus (rsv), influenza a and b, adenovirus, and parainfluenza 1 and 3. vir ... | 1989 | 2547019 |
| proteolytic activity of specialized surface protrusions formed at rosette contact sites of transformed cells. | surface protrusions at the leading edge of a moving cell that make contact with the surrounding extracellular matrix (ecm) are its main motor for locomotion and invasion. chicken embryonic fibroblasts transformed by rous sarcoma virus (rsv-cef) form specialized membrane rosette-shaped contact sites on planar substrata as shown by interference reflection microscopy (irm). such activity is lacking in normal cells. these rosette contacts are more labile than other adhesion sites, such as focal and ... | 1989 | 2549171 |
| [differences in the distribution of proviruses in low- and highly- metastatic variants of hamster cells, transformed by rous sarcoma virus]. | the structure of provirus in syrian hamster cells, transformed by rous sarcoma virus (rsv) varying in metastatic capability in vivo has been analysed. the original cell line and its low metastatic variants contain only one copy of the integrated rsv genome. the dna of highly metastatic cell lines cloned from the same primary culture, contain an additional copy of provirus. this rsv copy in different highly metastatic variants has a similar integration site. | 1989 | 2549401 |
| thermodynamic stability and statistical significance of potential stem-loop structures situated at the frameshift sites of retroviruses. | rna stem-loop structures situated just 3' to the frameshift sites of the retroviral gag-pol or gag-pro and pro-pol regions may make important contributions to frame-shifting in retroviruses. in this study, the thermodynamic stability and statistical significance of such secondary structural features relative to others in the sequence have been assessed using a newly developed method that combines calculations of the lowest free energy of formation of rna secondary structures and the monte carlo ... | 1989 | 2549508 |
| creation and expression of myristylated forms of rous sarcoma virus gag protein in mammalian cells. | rous sarcoma virus (rsv), a member of the avian sarcoma and leukosis family of retroviruses, has long been known to be capable of infecting and transforming mammalian cells; however, such transformed cells do not release virus particles. the rsv gag product (pr76gag) produced in these cells is not released into the culture medium or proteolytically processed to release mature products. thus, the behavior of pr76gag in mammalian cells is much like that of mammalian retroviral gag proteins which h ... | 1989 | 2550669 |
| expression vector system based on the chicken beta-actin promoter directs efficient production of interleukin-5. | we examined the promoter activity of the 1.3-kb chicken beta-actin gene sequence located between the 5' flanking region and the proximal region of the second exon. this promoter region showed higher promoter activity than the simian virus 40 (sv40) early promoter or the rous sarcoma virus (rsv) long terminal repeat (ltr) as assayed by transient lacz gene expression in mouse l cells. furthermore, replacement of the 3' splice sequence in this promoter by that derived from the rabbit beta-globin ge ... | 1989 | 2551778 |
| effects of alterations in the leader sequence of rous sarcoma virus rna on initiation of translation. | the 372-nucleotide leader sequence of rous sarcoma virus rna contains three conserved short open reading frames and other sequences responsible for a variety of life cycle functions. we have investigated several aspects of the leader rna which may influence the translation of the major coding regions to which the leader is juxtaposed. we found that small perturbations of the leader length do not affect the binding and scanning of ribosomal subunits by more than about 10%, that the length and/or ... | 1989 | 2552182 |
| tpck and quercetin act synergistically with vanadate to increase protein-tyrosine phosphorylation in avian cells. | we have found four compounds that act synergistically with the phosphotyrosine phosphatase inhibitor sodium orthovanadate (na3vo4) to greatly increase the extent of protein-tyrosine phosphorylation in both uninfected chick embryo fibroblasts (cef) and their rous sarcoma virus-transformed counterparts (rsv-cef). these four inhibitors fall into two categories: the chymotrypsin-specific protease inhibitors, tosyl-phenylalanine-chloromethyl ketone (tpck) and n-carbobenzoxy-1-phenylalanine-chlorometh ... | 1989 | 2552375 |
| a continuous line of chicken embryo cells derived from a chondrocyte culture infected with rsv. | a continuous line of chicken embryo cells was derived from a culture of chondrocytes infected with rous sarcoma virus (rsv). these cells, designated as na101, have reduced serum requirements and are able to grow in a semisolid medium. na101 cells show the same phenotype as freshly rsv infected chondrocyte cultures, i.e. synthesis of fibronectin and type i collagen, elongated bipolar shape and absence of tumorigenicity following grafting onto the chorioallantoic membrane of embryonated duck eggs; ... | 1989 | 2553220 |
| inhibition of avian retrovirus protein synthesis in the presence of host cellular mrna. | two conditions of in vitro translation are described in which the rna of rous sarcoma virus is significantly less efficient at protein synthesis than host cellular chick mrna. in direct competition, addition of increasing amounts of host cell mrna inhibits synthesis of gag gene products from rsv rna. when the denaturant dimethyl sulfoxide is added to a mixture of the two rna's the inhibition is even greater. these results support the idea that protein synthesis from avian retrovirus rna is negat ... | 1989 | 2554857 |
| inhibition of sv40 dna replication by rous sarcoma virus ltr enhancer. | simian virus 40 (sv40) late region recombinant constructs containing the rous sarcoma virus (rsv) src gene along with rsv enhancer stimulated expression but completely abolished sv40 dna replication. constructs, in which the heterologous enhancer sequences were omitted, did replicate normally in african green monkey kidney cells and, in the presence of helper virus, gave rise to infectious progeny. inhibition of sv40 dna replication follows a cis-acting mechanism and is most likely due to a conf ... | 1989 | 2554858 |
| clustering of discrete cell properties essential for tumorigenicity and metastasis. ii. studies of syrian hamster embryo fibroblasts transformed by rous sarcoma virus. | tumorigenic and metastasizing activities (tga; ma) and susceptibility, or resistance to h2o2 and pge-releasing activity (h2o2r + pges+ phenotype) have been examined in 6 syrian hamster embryo cell strains transformed in vitro with rous sarcoma viruses (schmidt-ruppin and prague strains). early observations of extremely high level of tga and even ma of rsv-sr-transformants never selected in vivo have been confirmed. the correspondence of these properties with a high level of expression of h2o2r + ... | 1989 | 2555309 |
| lipid characteristics of rsv-transformed balb/c 3t3 cell lines with different spontaneous metastatic potentials. | to determine whether a metastatic phenotype may be correlated with a characteristic lipid pattern, we compared the lipid composition of low metastasizing balb/c 3t3 cells transformed by the b77 strain of rous sarcoma virus (b77-3t3 cells) with that of a subclone isolated by growth in 0.6% agar, the b77-aa6 cells, which exhibit a high capacity for spontaneous metastasis. b77-3t3 cells revealed characteristics in their lipid composition common to other systems of transformed cells, i.e., an accumu ... | 1989 | 2555644 |
| cdna sequence of the long terminal repeat of a prb strain of the rous sarcoma virus. | the cdna library of the polya+ rna from prague b strain of rsv infected cells was constructed by the use of oligo(dt) primer and the reverse transcription method. the clones containing the ltr sequences were studied. the dna sequences of several clones were studied and compared with the cdna sequence of the long terminal repeat (ltr) region of a prb strain of rous sarcoma virus (rsv) which had previously been determined. by the analyses of several different cdna clones, mutations were detected i ... | 1989 | 2556723 |
| v-src induces clonal sarcomas and rapid metastasis following transduction with a replication-defective retrovirus. | v-src is an effective carcinogen when expressed from rous sarcoma virus (rsv) in vivo. whereas rsv tumors require sustained oncogene expression, their growth is largely a balance between viral recruitment of tissues and host immune destruction of infected cells. we have therefore examined the tumorigenic potential of v-src in the absence of viral recruitment and viral antigen expression. v-src was introduced with high efficiency into chicken wing web tissues using replication-defective (rd) retr ... | 1989 | 2557619 |
| [the family of env genes of avian retroviruses: molecular analysis of rous sarcoma virus adapted to duck cells]. | for the elucidation of the molecular basis of rsv adaptation to conditionally permissive host from the genome library of duck embryo fibroblasts, transformed by rous sarcoma virus in 30 passages on these cells, recombinant bacteriophages that include provirus sequences, were obtained. complete and transformation-defective proviruses were characterized, nucleotide sequences of their env-genes were compared with their counterparts the original rsv (pr-rsv-c) and with viruses of other subgroups (a, ... | 1989 | 2558292 |
| rescue of infectious virus from nonproducer rous cells by chick cellular dna. | the rescue of infectious virus from nonproducer bh rsv(-) cells by chick cellular dna was attempted in order to investigate the functional state of endogenous and exogenous retroviral genes integrated within the cellular dna. no infectious virus was rescued by transfection with dnas of chick helper factor (chf)-negative chick embryo cells (cec), chf-positive cec or uninfected cec producing endogenous rous associated virus (rav-0). on the other hand, infectious rous viruses with the phenotype of ... | 1989 | 2558502 |
| alkaline phosphatase fusions to the respiratory syncytial virus f protein as an approach to analyze its membrane topology. | manoil and beckwith (1985) have constructed a transposon, tnphoa, that permits the generation of hybrid proteins composed of alkaline phosphatase (ap) lacking its signal peptide fused to amino-terminal sequences of other proteins. this transposon has been used to localize export signals and analyze membrane topology of bacterial proteins. we have applied this approach to the membrane fusion protein (f) of respiratory syncytial virus (rsv). the transposon tnphoa and a plasmid directing bacterial ... | 1989 | 2558867 |
| potential usefulness of a cultured glioma cell line induced by rous sarcoma virus in b10.a mouse as an immunotherapy model. | a cultured glioma cell line, sr-b10.a, which was derived from a brain tumor induced in an adult female b10.a mouse by rous sarcoma virus (rsv), has been established. the morphological appearance of the tumor produced by s.c. inoculating sr-b10.a cells was analogous to an astrocytoma of human glioma. glial fibrillary acidic protein as well as s-100 protein was positive in these sr-b10.a tumor cells. a population doubling time of the cultured cells was 18.5 hours. chromosomal analysis revealed a d ... | 1989 | 2559217 |
| cellular immunity in chicks expressing fibrosarcomatous liver tumor following embryo infection with subgroup a rous sarcoma virus. | bryan standard strain of rous sarcoma virus (bs-rsv) of subgroup a was inoculated into heavy and light breeds of chicken embryos via chorioallantoic membrane (cam) to ascertain cell-mediated immune response, as measured by a leukocyte migration inhibition (lmi) test. chicks hatched from eggs with pock-positive cams were more likely to develop liver tumors than those hatched from eggs with pock-negative cams. chicks that developed tumors usually had a positive cell-mediated immune response, and t ... | 1989 | 2559698 |
| expression of a prokaryotic gene in cultured lung endothelial cells after lipofection with a plasmid vector. | transfection of cultured cells with functioning foreign genes can be a powerful tool for delineating mechanisms controlling gene expression and for manipulating cellular synthetic machinery. we transfected cultured bovine pulmonary artery endothelial cells with a plasmid (prsvcat) containing the prokaryotic gene, chloramphenicol acetyltransferase (cat), driven by a rous sarcoma virus (rsv) promoter. transfection was accomplished by binding the plasmid dna to specially synthesized cationic liposo ... | 1989 | 2559763 |
| insulin enhances glucocorticoid induction of gene expression in a sequence specific manner. | glucocorticoid receptor (gr) induces transcriptional activation of specific genes by binding to cis-acting enhancer sequences termed glucocorticoid responsive elements (gres). insulin regulates gene transcription part of which is mediated sequences dominated by gres. by transient expression in htc cells of fusion gene consist mmtv 5' flanking sequences containing the gres on the thymidine kinase promoter (tk) and the coding region of the chloramphenicol acetyl transferase gene (cat), we demonstr ... | 1989 | 2560516 |
| a variant schmidt-ruppin strain of rous sarcoma virus with increased affinity for mammalian cells. | sr-rsv-d(h), a variant virus with extremely high tropism for mammalian cells, was isolated by passage of the schmidt-ruppin strain of rous sarcoma virus of subgroup d (sr-rsv-d) through hamster cells. this variant virus has acquired an altered envelope glycoprotein, encoded by the env gene, that has high affinity for receptors on the surface of mammalian cells. the variant virus transforms rat cells at about 100 times the efficiency of the parental virus, sr-rsv-d(s), as assayed by focus formati ... | 1989 | 2560773 |
| augmentation of anti-tumor immunity in low-responder mice by various biological response modifiers: analysis of effector mechanism. | in order to elucidate the role of biological response modifiers (brms) in anti-tumor immunotherapy, we examined their effect on the induction of anti-tumor immunity in low-responder mice which hardly exhibit anti-tumor resistance against syngeneic rous sarcoma virus (rsv)-induced tumors, such as b10 or b10.br mice. the anti-tumor immunity induction in the low-responder mice was 0% on immunization with mitomycin c-treated syngeneic tumor cells alone. however, if brms were used as an adjuvant, bcg ... | 1989 | 2560774 |
| analysis of b-g and immune response genes in the iowa state university s1 chicken line by hybridization of sperm deoxyribonucleic acid with a major histocompatibility complex class ii probe. | sperm dna was isolated from chickens of the iowa state university s1 line. birds were from sublines selected for b-g antigen, humoral immune response to glutamic acid-alanine-tyrosine (irgat), and response to rous sarcoma virus-induced (rsv) tumors. the dna was digested with restriction enzymes and subjected to southern blot analysis with a dna probe specific for a class ii gene of the chicken major histocompatibility complex (mhc). restriction fragment length polymorphisms (rflp) associated wit ... | 1989 | 2565033 |
| occurrence and aetiology of acute respiratory diseases: results of a longterm surveillance programme. | totals of 58,661,000 acute respiratory disease (ard) cases, 1,376,651 bronchitis and pneumonia complications, and 93,042 deaths from influenza, bronchitis, pneumonia or chronic pulmonary affection were notified during 11 years of ard surveillance from 1975 to 1986. all ard seasons started with the first phase in september-december; this increase in morbidity was caused chiefly by adenoviruses, parainfluenza viruses, rhinoviruses and m. pneumoniae. second wave of ard morbidity occurring in januar ... | 1989 | 2565674 |
| synthesis of respiratory syncytial virus rna in cell-free extracts. | a cell-free system has been used to synthesize respiratory syncytial virus (rsv) rna in vitro. polyadenylated species representing all size classes of rsv mrnas were labelled. some of the labelled rna was seen in a cscl gradient at the density characteristic of negative-strand viral nucleocapsids. experiments using a thiotriphos-phorylated nucleotide indicate that rna chains are initiated de novo in the cell-free system. | 1989 | 2567342 |
| increase in ribosomal protein s6 phosphorylation is due to v-erbb-transforming activity and not to v-erba mitogenic activity in avian erythroblastosis virus-infected chicken embryo fibroblasts. | avian erythroblastosis virus (aev-es4), a transforming avian retrovirus, transforms chicken embryo fibroblasts (cefs) in culture and induces the maintenance of ribosomal protein s6 phosphorylation in the absence of serum. this effect is less pronounced after aev-es4 transformation than after transformation by rous sarcoma virus (pr-rsv a). however, our results indicate that the two viruses induce an activation of the same s6 phosphokinase, as evidenced by the identity of s6 phosphopeptides and p ... | 1989 | 2567979 |
| effects of norakin on respiratory syncytial virus in tissue culture and in mice. | norakin at 1 microgram/ml inhibits the reproduction of respiratory syncytial virus (rsv) in vero cells to 50% and at 5 micrograms/ml to 90%. the development of lung lesions in rsv-infected balb/c mice was suppressed by 70% when the animals were treated with two doses (25 mg/kg each) of norakin, 30 min before and 4 hr post infection. (p.i.), respectively. | 1989 | 2569812 |
| enhancement of immunity against rsv-induced sarcomas by generation of hapten-reactive helper t lymphocytes. | previous work from this laboratory has shown that preimmunization of syngeneic hosts with rous sarcoma virus (rsv)-transformed cells elicits a strong immune response against the growth of transplantable rsv sarcomas, mediated by t lymphocytes expressing the surface phenotype of helper cell precursors (prat, di renzo & comoglio, 1983). this paper shows that anti-tumour immunity may be elicited in tumour-bearing animals by triggering an experimentally pre-amplified t-helper cell population at the ... | 1985 | 2578431 |
| antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. | to study the antigenic characteristics of respiratory syncytial virus (rsv), we developed and evaluated monoclonal antibodies (mabs) to three strains of rsv: 11 to long, 4 to 18537, and 9 to a2. six of these mabs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion protein. by the pattern of the reactions of these mabs to 16 strains of rsv in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay, we were able to distinguish three subgroups. with ... | 1985 | 2579169 |
| antigenic analysis of a putative new strain of respiratory syncytial virus. | a recent isolate (9320) of respiratory syncytial virus (rsv) that had been reported to differ from all previous strains was compared with rsv strains isolated over a period of 23 years by using the techniques of in vitro neutralization by specific antiserum and in vivo resistance to infection in cotton rats. the 9320 strain did not appear to be a novel antigenic variant and exhibited greater homology to several prototype strains than did an earlier isolate. the antigenic differences detected by ... | 1985 | 2579170 |
| serum antibodies against respiratory tract viruses: a prospective three-year follow-up from birth. | acute otitis media (aom) has been epidemiologically related to viral respiratory tract infections, and viral antigens have also been detected in middle ear secretion in some aom episodes. successive serum samples from children followed prospectively for three years from birth were analysed for igg antibodies against respiratory syncytial virus (rsv), adenoviruses and influenza a virus. values from serum antibody activity gradually decreased during the first six months of life, followed by a grad ... | 1989 | 2584849 |
| respiratory syncytial virus: a nursing perspective. | respiratory syncytial virus (rsv), a common winter illness, is most devastating in infants with underlying pulmonary and cardiovascular disease. ribavirin aerosol is an effective treatment when used with appropriate nursing management. | 1989 | 2587088 |
| detection of multiple viral agents in nasopharyngeal specimens yielding respiratory syncytial virus (rsv). an assessment of diagnostic strategy and clinical significance. | a retrospective study of 6 years (1981-1987) experience with clinical specimens of pediatric patients submitted for identification of respiratory viruses was undertaken to determine the prevalence of multiple viral isolates and to assess the impact of dual infections on severity of clinical disease. respiratory syncytial virus (rsv), the most frequently identified agent, was detected in cell culture and/or by immunofluorescence (if) in 666 of 2,415 specimens examined. a second virus was isolated ... | 1989 | 2591168 |
| hla class i antigens in severe rsv bronchiolitis. | cytotoxic t-lymphocytes (ctl) recognize virus-infected cells in association with hla class i antigens. there is strong evidence of the importance of ctl in respiratory syncytial virus (rsv) infections. we looked for but were unable to demonstrate an association between particular hla class i antigens and severe rsv bronchiolitis in infants. | 1989 | 2595727 |
| [severe respiratory syncytial virus infections. study of 87 infants hospitalized in an epidemic]. | during a winter epidemic, 87 infants were admitted to necker-enfants-malades hospital with a severe respiratory syncitial virus (rsv) infection. these infants fell into two groups: 37 infants without any medical history and 50 showing an underlying pathology (immune deficiencies, heart disease, cns disorders, digestive malformations, allergic manifestations). of the 37 infants with no medical history, most were below the age of 6 months and the rsv infection was manifested clinically by bronchio ... | 1989 | 2604509 |
| antiviral activities and the mechanism of 9-beta-d-ribofuranosyl-6-alkylthiopurines on several rna viruses from animals. | a series of 9-beta-d-ribofuranosyl-6-alkylthiopurines (6-alkyl ti) were found to inhibit in vitro replication of infectious hematopoietic necrosis virus (ihnv), human influenza virus (ifv) and respiratory syncytial virus (rsv) with ic50 values of about 0.06 microgram/ml, 0.7-1.5 micrograms/ml and 1-3 micrograms/ml, respectively. viral rna synthesis in infected cells in the presence of actinomycin d was inhibited by treatment with the compounds dose-dependently. it was also found that the decreas ... | 1989 | 2608474 |
| the 1a protein of respiratory syncytial virus is an integral membrane protein present as multiple, structurally distinct species. | the respiratory syncytial virus (rsv) 1a protein was previously identified as a 7.5-kilodalton (kda) nonglycosylated species that, on the basis of its predicted sequence determined from the sequence of its mrna, contains a hydrophobic central domain that was suggestive of membrane interaction. here, four major, structurally distinct intracellular species of the 1a protein were identified in cells infected by rsv or by a recombinant vaccinia virus expressing the 1a gene. the four species of 1a we ... | 1989 | 2649692 |
| comparison of the anti-respiratory syncytial virus activity and toxicity of papaverine hydrochloride and pyrazofurin in vitro and in vivo. | based on reports describing their broad antiviral activity, the toxicity and antiviral efficacy of papaverine hydrochloride and pyrazofurin against respiratory syncytial virus (rsv) infection were tested in vitro in tissue culture cells and in vivo in cotton rats. papaverine inhibited rsv replication in vitro; however, the median minimal toxic dose-median minimal inhibitory concentration ratios (mtd50:mic50) in vitro and in vivo for papaverine were less than 4. further work with this compound wa ... | 1989 | 2653219 |
| use of high-speed rolling to detect respiratory syncytial virus in cell culture. | we examined the effect of motion on respiratory syncytial virus (rsv) growth in cell culture. infected cultures were incubated stationary, rolling, or on an orbital shaker. enzyme immunoassay (eia) results for cultures infected with high concentrations of a laboratory strain of rsv were similar for all incubation conditions. however, cultures infected with low concentrations of virus and rolled at 96 rpm had a significantly greater mean eia optical density (1.78 +/- 0.22) than cultures rolled at ... | 1989 | 2654185 |
| respiratory syncytial virus alters electrophysiologic properties in cotton rat airway epithelium. | the effect of respiratory syncytial virus (rsv) infection on the electrophysiologic properties of the airway epithelium was studied in tracheas obtained from cotton rats, after in vivo exposure to the virus. rsv infection was documented by tissue culture infectivity and immunofluorescent antibody techniques. light microscopic studies of the tracheas 72 hours after exposure to rsv revealed normal epithelial morphology. rsv infection produced a 28% decrease in short-circuit current (isc) and an al ... | 1989 | 2654848 |
| mechanism of lung injury in cotton rats immunized with formalin-inactivated respiratory syncytial virus. | respiratory syncytial virus (rsv) seronegative cotton rats were immunized intramuscularly at four and eight weeks of age with either formalin-inactivated, alum-precipitated rsv grown in hep-2 cell tissue cultures or virus-free hep-2 cell tissue cultures similarly prepared. sham-immunized animals served as controls. at 12 weeks of age, all animals were challenged with 6 x 10(5) plaque forming units of live rsv via the intranasal route. animals were killed at predetermined days and evaluated for r ... | 1989 | 2655343 |
| a sensitive technique to monitor gene transfer and expression in bone marrow stem cells. | the polymerase chain reaction technique (pcr), a primer-mediated enzymatic amplification of specific target sequences, was used to monitor gene transfer into hematopoietic progenitor stem cells. a gene coding for human interleukin 1 alpha (il-1 alpha) was cotransfected with the rous sarcoma virus (rsv) cat plasmid into mouse bone marrow cells by electroporation. individual chloramphenicol (cam)-resistant bone marrow progenitor colonies (granulocyte-macrophage colony-forming units; cfu-gm) contai ... | 1989 | 2666145 |
| evaluation of the abbott testpack rsv enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. | the abbott testpack rsv assay (abbott laboratories, north chicago, ill.), a rapid (20-min) enzyme immunoassay, was compared with culture and direct immunofluorescence (dfa) of nasopharyngeal cells for the detection of respiratory syncytial virus (rsv) in nasopharyngeal swab specimens. nasopharyngeal swab specimens, collected from 234 infants, were placed in viral transport medium. portions of specimen in transport medium were used for each test. of 234 specimens, 70 (30%) were culture positive, ... | 1989 | 2666434 |
| development of cell-mediated cytotoxic immunity to respiratory syncytial virus in human infants following naturally acquired infection. | with virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (rsv) was studied in peripheral blood lymphocytes (pbl) in groups of infants with acute rsv infection and in other control groups of subjects during a community outbreak of rsv infection. no rsv-specific cellular cytotoxicity was observed in cord blood lymphocytes and in other uninfected controls. the pbl of infants with acute rs ... | 1989 | 2666570 |
| antigenic characterization of respiratory syncytial virus associated with acute respiratory infections in uruguayan children from 1985 to 1987. | the occurrence of subgroup a and b strains of respiratory syncytial virus (rsv) was studied during three epidemic years, 1985 to 1987, in uruguay. a set of monoclonal antibodies was selected according to their reactivity with local rsv isolates and used for the typing of rsv directly in nasopharyngeal cells by indirect immunofluorescence. of 77 specimens, 69 could be typed as belonging to subgroup a or b, 5 could not be typed with the restricted set of monoclonal antibodies employed, and 3 react ... | 1989 | 2671013 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by dna-rna hybridization. | we have developed an rna-cdna hybridization assay for the detection of respiratory syncytial virus (rsv) rna in nasopharyngeal samples. we chose to use as probe a cdna complementary to the nucleocapsid protein gene of rsv, integrated into the plasmid vector pbr322. the lower limit of sensitivity of the assay is 8.2 x 10(2) pfu of the long strain of rsv. in throat washes with added cell-free virus, the assay can detect 3.3 x 10(3) pfu of rsv. respiratory secretions were collected from a group of ... | 1989 | 2671029 |
| immunosuppression of the antibody response to respiratory syncytial virus (rsv) by pre-existing serum antibodies: partial prevention by topical infection of the respiratory tract with vaccinia virus-rsv recombinants. | immunization strategies to prevent respiratory syncytial virus (rsv) disease will involve immunization of infants less than 2 months of age who possess maternally derived rsv antibodies. vaccinia-rsv recombinant viruses are useful tools for defining parameters important in immunization against rsv and also are being considered as live virus vaccines for use in humans. previous studies demonstrated that passively acquired rsv antibodies can suppress the immune response and the protective efficacy ... | 1989 | 2671259 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and elisa does not justify discontinuation of virus isolation. | direct fluorescent antibody assay (dfa) using monoclonal antibody and enzyme-linked immunosorbent assay (elisa) for rapid detection of respiratory syncytial virus (rsv) in nasopharyngeal secretions (nps) were compared with conventional virus isolation and identification procedures in cell cultures. when 134 nps were examined from infants and young children with acute respiratory tract infection, 42 (31%) were culture-positive for rsv and 31 of these were detected by the appearance of a typical c ... | 1989 | 2674634 |
| viral etiology in acute lower respiratory infections in children from a closed community. | the aim of this study was to determine the viral agents associated with acute lower respiratory infections (alri) in young children. during a 2-yr period, 204 nasopharyngeal aspirates (npa) from children under 4 yr of age living in an orphanage and exhibiting febrile alri were studied by both indirect immunofluorescence (iif) and isolation in four cell lines. npa cell smears as well as tissue culture cells exhibiting cytopathic effect (cpe) or hemadsorption were stained by iif for respiratory sy ... | 1989 | 2675703 |
| protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric fg glycoprotein. | the cotton rat model of experimental human respiratory syncytial virus (rsv) infection was used to study the efficacy of fg, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. fg contained the extracellular regions of the f (fusion) and g (attachment) glycoproteins of rsv. vaccination with fg resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from rsv challenge against both serogroup a and b virus strains. b ... | 1989 | 2677234 |
| respiratory syncytial virus-induced acute lung injury in adult patients with bone marrow transplants: a clinical approach and review of the literature. | acute lung injury induced by respiratory syncytial virus (rsv) is a major cause of morbidity and mortality in patients who have undergone bone marrow transplantation. twenty-nine of the 74 patients who received bone marrow transplants at the university of minnesota during a 1-year period developed evidence of acute lung injury, and rsv was identified as the cause in 8. we discuss the clinical course of these 8 patients and offer a clinical approach to rsv infection occurring after bone marrow tr ... | 1989 | 2677595 |
| [outbreak of respiratory syncytial virus in a neonatal unit]. | described is an outbreak of nosocomial rsv infection, following the admission of a 20-day-old and 11-day-old newborn with lower respiratory infections, diagnosed as being caused by rsv through detection of viral antigens by direct fat (fluorescent antibody test) of nasopharyngeal samples. later on, 5 of the other 20 newborn in patients in the same care unit also showed positive results in direct fat, for rsv. the clinical manifestations of these patients were mild even in those cases with underl ... | 1989 | 2679277 |
| eukaryotic promoters drive gene expression in escherichia coli. | eight eukaryotic promoters have been tested for their activity in vivo in escherichia coli. the rat beta-actin, rat amylase, rat chymotrypsin b, mouse metallothionein i, rat insulin i, human insulin, rous sarcoma virus long terminal repeat (rsv ltr) and hepatitis b viral precore promoter activities were measured by using the bacterial chloramphenicol acetyltransferase coding sequences as the reporter function and by primer extension rna analysis. all eight promoter-chloramphenicol acetyltransfer ... | 1989 | 2681182 |
| immunization of cotton rats with the fusion (f) and large (g) glycoproteins of respiratory syncytial virus (rsv) protects against rsv challenge without potentiating rsv disease. | a formalin-inactivated respiratory syncytial virus (rsv) vaccine tested 22 years ago failed to protect infant vaccinees against rsv infection or disease. instead, lower respiratory tract disease was enhanced during subsequent infection by rsv. enhancement of pulmonary pathology is also observed when cotton rats are immunized with formalin-inactivated rsv and subsequently infected with this virus. a major question that must be addressed for each new paramyxovirus vaccine is whether the immunogen ... | 1989 | 2692334 |
| rapid detection of respiratory syncytial virus by a biotin-enhanced immunoassay: test performance by laboratory technologists and housestaff. | a biotin-enhanced enzyme immunoassay (eia) for respiratory syncytial virus (rsv) antigen detection (testpack rsv) was prospectively compared with virus isolation in cell culture and immunofluorescence. of 156 nasopharyngeal swab specimens from infants with respiratory symptoms, 81 (52%) yielded rsv in culture. compared with culture the sensitivity of the eia was 95% and specificity was 92%; the specificity increased to 97% with a blocking assay. compared with immunofluorescence the sensitivity o ... | 1989 | 2696927 |
| [effect of the environment on the development of respiratory allergies]. | allergy reactions may be regarded as resulting from exposure to allergens of subjects who are genetically predisposed to synthesize ige. the presence of allergens in the environment is necessary to acquire sensitization. with some allergens, sensitization seems to develop very rapidly. the introduction or presence in the environment of certain allergens, such as house dust mites, may increase the prevalence of asthma. despite conflicting reports, in atopic subjects exposure to pet animals also i ... | 1989 | 2699066 |
| serum immunoglobulin g antibody subclass response to respiratory syncytial virus f and g glycoproteins after first, second, and third infections. | serum samples from 31 children who experienced two or three infections with respiratory syncytial virus (rsv) in the first four years of life were tested in an enzyme-linked immunosorbent assay to examine the immunoglobulin g (igg) subclass responses to the rsv f and g surface glycoproteins associated with primary infection and reinfection. we sought to determine whether the greater degree of glycosylation of the g glycoprotein was reflected in an igg subclass immune response more like that to a ... | 1989 | 2715331 |
| characterization of prolonged apneic episodes associated with respiratory syncytial virus infection. | we used high-speed polygraphic recordings to document in detail the sequence of events during prolonged apneic spells in two infants with respiratory syncytial viral (rsv) infection. also, we elicited upper airway reflexes by stimulating the airway with saline during sleep. spontaneous prolonged apneic spells were recorded in both infants, and these consisted of two distinctly different apnea types. a number of similarities between apnea of prematurity, upper airway reflex apnea and the apneas i ... | 1989 | 2717245 |
| immunoglobulin-class-specific immune response to respiratory syncytial virus structural proteins in infants, children, and adults. | the protein specificities of igg, igm, and iga antibodies induced during respiratory syncytial virus (rsv) infection in 74 patients (4 weeks to 81 years of age) were investigated using the technique of immunoblotting. although the pattern of antibody reactivity varied among patients, most of the humoral immune response in all age groups was directed against the 48, 42, 35, and 27 k proteins. an infant's own antibody response was discernible in 55 of the 57 children below 1 year of age, despite t ... | 1989 | 2723614 |
| immune response to experimentally induced infection with respiratory syncytial virus: possible role in the development of pulmonary disease. | groups of mice were immunized intraperitoneally with hep-2 lysate or respiratory syncytial virus (rsv)-infected hep-2 cells with or without adjuvant(s). the animals were subsequently challenged intranasally (i.n.) with rsv and/or hep-2 lysate and studied for rsv shedding in the lung, antigen-specific antibody response and pulmonary histopathology. a significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups t ... | 1989 | 2732693 |
| induction of in vitro differentiation of mouse embryonal carcinoma (f9) and erythroleukemia (mel) cells by herbimycin a, an inhibitor of protein phosphorylation. | herbimycin a is one of the benzenoid ansamycin antibiotics isolated from a culture of streptomyces species (omura, s., a. nakagawa, and n. sadakane. 1979. tetrahedron lett. 1979: 4323-4326). recent studies have shown that the antibiotic not only inhibits the phosphorylation of p60src in rous sarcoma virus- (rsv) infected cells, but also reverses the cellular phenotypes acquired by transfection with tyrosine kinase oncogenes (uehara, y., m. hori, t. takeuchi, and h. umezawa. 1985. jpn. j. cancer ... | 1989 | 2745553 |
| bronchial reactivity in infants in acute respiratory failure with viral bronchiolitis. | airway reactivity and the effects of bronchodilators in infants are controversial. we studied the response to bronchodilator treatment in 14 mechanically ventilated infants (mean age, 2.74 months; range, 0.6-5.9) in respiratory failure caused by respiratory syncytial virus (rsv)-associated bronchiolitis. sixteen infants without lung disease, undergoing elective surgery, provided normal values. maximum expiratory deflation flow-volume (dfv) curves were produced by manual inflation of the lungs wi ... | 1989 | 2748221 |
| [respiratory insufficiency in acute bronchiolitis in infancy]. | forty-one infants with acute viral bronchiolitis were hospitalized in our paediatric intensive care unit during the seven year period from 1980 to 1987. in 14 out of 27 evaluated patients, respiratory syncitial virus (rsv) was detected in the nasal secretions. twenty-three children required only supportive care and monitoring. eighteen infants had to be ventilated because of respiratory failure. the major indication for mechanical ventilation was an arterial or capillary pco2 of more than 64 mmh ... | 1989 | 2755928 |
| prevalence of respiratory syncytial virus subgroups over six consecutive outbreaks: 1981-1987. | indirect immunofluorescence with strain-specific monoclonal antibodies was used to determine the phenotype of respiratory syncytial virus (rsv) isolates obtained from infants hospitalized in greater boston over six successive outbreaks from 1981 to 1987. of 981 isolates, 591 (60%) were classified as subgroup a and 383 (39%) as subgroup b. the prevalence of subgroups varied both between and within yearly outbreaks. in 1983-84 and 1984-85, both subgroups circulated concurrently and in almost equal ... | 1989 | 2760479 |
| the frequency, distribution and effects of antibodies, to seven putative respiratory pathogens, on respiratory disease and weight gain in feedlot calves in ontario. | during 1983-85, 279 calves requiring treatment for bovine respiratory disease and 290 comparison (control) animals from 15 different groups of feedlot calves were bled on arrival and again at 28 days postarrival. their sera were then analyzed for antibodies to seven putative respiratory pathogens. on arrival, the prevalences of indirect agglutination titers to pasteurella haemolytica, p. haemolytica cytotoxin, mycoplasma bovis and m. dispar were greater than 50%, the prevalence of titers to bovi ... | 1989 | 2766158 |
| evidence that the fusion protein of respiratory syncytial virus exists as a dimer in its native form. brief report. | the quaternary structure of respiratory syncytial virus (rsv) fusion protein has been studied. crosslinking studies were done to stabilize the noncovalently associated proteins. these stable, heat-resistant, covalently linked complexes were analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. in situ crosslinking studies demonstrated that the fusion protein of rsv exists as a dimer in its native form on the surface of infected cells. the purified protein was also found to be presen ... | 1989 | 2774976 |
| immunoglobulin a antibody response to respiratory syncytial virus structural proteins in colostrum and milk. | immunoglobulin a (iga) antibody response to respiratory syncytial virus (rsv) structural proteins in colostrum and milk was investigated by a radioimmunoprecipitation assay. by using [35s]methionine-labeled rsv-infected hep-2 cells and antiserum to human iga as the capture antibody, iga antibody responses to large glycoprotein, fusion protein, nucleoprotein, phosphoprotein, and matrix protein were demonstrated in colostrum and milk. the iga antibody response was mainly directed against fusion pr ... | 1989 | 2778061 |
| flow cytometric analysis of pulmonary lymphocytes from mice infected with respiratory syncytial virus. | balb/c mice were infected intra-nasally with respiratory syncytial virus (rsv) and cells recovered from the lungs by single or repeated bronchoalveolar lavage (bal). single bal gave enough cells (1 - 2 x 10(5) cells/mouse) for morphological study of giemsa stained cytospin preparations, and allowed the same mouse lungs to be assayed for virus titre or processed for histology. in normal or sham-infected mice the majority of recovered cells were macrophages, with less than 5% lymphocytes. followin ... | 1989 | 2784741 |
| interleukin-1 inhibitor production by human mononuclear leukocytes and leukocyte subpopulations exposed to respiratory syncytial virus: analysis and comparison with the response to influenza virus. | net interleukin-1 (il-1) inhibitor activity is induced by exposure of purified human monocytes-macrophages to respiratory syncytial virus (rsv). furthermore, il-1 inhibitor activity was produced by monocytes-macrophages exposed to rsv in the presence of lymphocytes, that is, by unseparated mononuclear leukocytes (mnl). purified rsv-exposed lymphocytes, as well as the lymphocytes exposed within mnl preparations, produced net il-1 inhibitor activity. in contrast, net il-1 activity was produced whe ... | 1989 | 2788203 |
| clinical characteristics of respiratory syncytial virus infections in healthy versus previously compromised host. | in an effort to delineate the clinical characteristics of respiratory syncytial virus (rsv) infection in the compromised host, we compared children with bronchopulmonary dysplasia (bpd), congenital heart disease (chd), premature birth, failure to thrive, and gastroesophageal reflux to previously healthy children. during a four-year period, 262 patients were admitted to the hospital with rsv infection diagnosed by a rapid rsv antigen detection test. children with bpd or chd had more hospital days ... | 1989 | 2797931 |
| duration of hospitalization in previously well infants with respiratory syncytial virus infection. | to describe the typical hospital course of infection in previously well infants hospitalized with respiratory syncytial virus (rsv) infection, we reviewed the charts of 196 patients with laboratory-proved respiratory syncytial virus infection in the 1987-1988 respiratory disease season. eighty-seven of the children had been previously well. their mean duration of hospitalization was 3.4 days. previously well infants younger than 6 weeks of age experienced significantly longer hospitalizations an ... | 1989 | 2797955 |
| effect of vaccination on cell populations in lung washes from calves after infection with respiratory syncytial virus. | the inflammatory response in the air-passages of the lungs of calves after intranasal inoculation with respiratory syncytial virus (rsv) was compared in rsv-vaccinated and control animals. total cells recovered from lung washings remained the same; however, the fold by eight days after infection and the type of cells changed from a predominance (85 per cent) of macrophages to equal proportions of macrophages and neutrophils (45 per cent) during the course of infection. the absolute numbers of ne ... | 1989 | 2799080 |
| hilar enlargement in respiratory syncytial virus pneumonia. | the clinical and radiographic features of ten children with hilar enlargement in association with proven respiratory syncytial virus (rsv) infection are described. hilar enlargement was seen in 10/35 children with rsv infection, and was invariably unilateral and right sided. it is recommended that rsv pneumonia be considered in children with unilateral hilar enlargement if tuberculosis has been excluded, and the onset of disease is rapid. | 1989 | 2806272 |
| antibody-mediated enhancement of respiratory syncytial virus infection in two monocyte/macrophage cell lines. | monoclonal antibodies (mabs) specific for two surface glycoproteins of respiratory syncytial virus (rsv) were found to enhance rsv infection in two macrophagelike cell lines (p388d1 and thp-1). mabs to an irrelevant antigen (pneumococcal polysaccharide) and to the nucleocapsid of rsv did not enhance infection. blocking either the fc segment of the monoclonal antibody of the fc receptor on the cells diminished the enhancement, suggesting that this phenomenon involves attachment of the monoclonal ... | 1989 | 2809253 |