Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [natural killers and cytotoxic lymphocytes in classical hog cholera]. | the formation of immune mechanisms directed at elimination of infected cells and including the activity of natural killers and cytotoxic lymphocytes was assessed in pigs infected with hog cholera virus. in acute disease natural killer activity in the blood is reduced, while in vaccinal process it is increased. vaccination in parallel with cyclophosphamide immunodepression lead to inhibition of natural killer activity. leukocytes and lymphocytes of immunized pigs can cause cytolysis of autologous ... | 1995 | 7483572 |
| an rna pseudoknot is an essential structural element of the internal ribosome entry site located within the hepatitis c virus 5' noncoding region. | translation of the human hepatitis c virus (hcv) rna genome occurs by a mechanism known as "internal ribosome entry." this unusual strategy of translation is employed by naturally uncapped picornaviral genomic rnas and several cellular mrnas. a common feature of these rnas is a relatively long 5' noncoding region (ncr) that folds into a complex secondary structure harboring an internal ribosome entry site (ires). evidence derived from the use of dicistronic expression systems, combined with an e ... | 1995 | 7489514 |
| african swine fever virus structural protein p72 contains a conformational neutralizing epitope. | we have previously described a monoclonal antibody (mab 135d4) to an unidentified 70- to 72-kda african swine fever virus (asfv) protein that exhibited high levels of neutralizing activity against various virulent asfv isolates. here, we identify the reactive asfv protein as the major virus structural protein p72. in vitro-translated products of the p72 protein gene were specifically immunoprecipitated by mab 135d4. immunoprecipitation of a nested set of truncated p72 in vitro translation produc ... | 1994 | 7514322 |
| antigenic structure of envelope glycoprotein e1 of hog cholera virus. | envelope glycoprotein e1 (gp51 to gp54) is the most antigenic protein of hog cholera virus or classical swine fever virus (csfv). four antigenic domains, a to d, have been mapped on e1 with a panel of monoclonal antibodies (mabs) raised against csfv strain brescia. the boundaries of these domains have been established by extensive studies on binding of mabs to transiently expressed deletion mutants of e1 (p. a. van rijn, e. j. de meijer, h. g. p. van gennip, and r. j. m. moormann, j. gen. virol. ... | 1994 | 7514680 |
| the influence of protease inhibitors of the organism, especially bovine aprotinin, on the production of virulent hog cholera virus in tissue cultures. | the influence of biological protease inhibitors, especially aprotinin, on the production of virulent hog cholera virus in cell cultures. production of number and size of fluorescent plaques after infection pk 15 cells with hc virus depended on properties of fetal calf sera added to the medium. by affinity chromatography on bovine alpha-chymotrypsin bound to cm-cellulose inhibitory proteins against chymotrypsin-like proteases could be eliminated from inhibiting sera. the fraction free from inhibi ... | 1994 | 7516855 |
| rapid characterization of new pestivirus strains by direct sequencing of pcr-amplified cdna from the 5' noncoding region. | reverse transcription coupled with the polymerase chain reaction (rt-pcr) was used for the rapid laboratory diagnosis of pestivirus infections. a direct dna sequencing method was developed for the analysis of the amplified cdna from the 5' noncoding region of the viral genome. 70 pestivirus strains were compared in this study. sequence analysis allowed the characterization of each isolate as either classical swine fever virus (csfv), bovine viral diarrhea virus, or border disease virus, respecti ... | 1994 | 7529990 |
| uses of flow cytometry in virology. | this article reviews some of the published applications of flow cytometry for in vitro and in vivo detection and enumeration of virus-infected cells. sample preparation, fixation, and permeabilization techniques for a number of virus-cell systems are evaluated. the use of flow cytometry for multiparameter analysis of virus-cell interactions for simian virus 40, herpes simplex viruses, human cytomegalovirus, and human immunodeficiency virus and its use for determining the effect of antiviral comp ... | 1994 | 7530594 |
| partial sequencing of hog cholera virus alfort strain genome and its comparison with other pestivirus strains. | after molecular rna cloning of the alfort strain (alfort/lcrv) of hog cholera virus (hcv), the nucleotide sequence of about 70% of the total genome was determined. this sequence was compared with homologous parts of previously published pestivirus genomes. the average homology with another clone of the alfort strain (alfort/frc) was found to be lower (86.1%) than with brescia strain of hcv (94.3%), while, compared with nadl, osloss and sd-1 (3 different strains of bovine viral diarrhea virus, bv ... | 1995 | 7550401 |
| pestivirus diversity. | 1995 | 7560298 | |
| pathological changes in the renal interstitial capillaries of pigs inoculated with two different strains of african swine fever virus. | african swine fever is a viral disease of pigs characterized predominantly by haemorrhagic lesions. this paper reports the lesions observed in the renal interstitial capillaries of pigs inoculated with african swine fever virus strains of differing virulence: the malawi'83 strain (haemadsorbent and highly virulent) and the dominican republic'78 strain (haemadsorbent and moderately virulent). in pigs infected with the malawi'83 strain, petechial haemorrhages and microhaemorrhages were observed 5 ... | 1995 | 7560303 |
| experimental african swine fever: apoptosis of lymphocytes and virus replication in other cells. | in order to determine the cause of cellular death of lymphocytes in pigs with acute african swine fever and the relationships between african swine fever virus (asfv) and interstitial cells, ten pigs were inoculated with a highly virulent strain of asfv (malawi '83) and samples taken for ultrastructural study of hepatic and renal interstitial tissues. we demonstrated death by apoptosis of lymphocytes and virus replication in fibroblasts, smooth muscle cells and endothelial cells in the interstit ... | 1995 | 7561784 |
| inactivation of viruses in liquid manure. | the stability of some viruses and methods of virus inactivation in liquid manure are reviewed. the authors discuss experimental data on the stability of foot and mouth disease virus, classical swine fever virus, aujeszky's disease virus, african swine fever virus, swine influenza virus, porcine paramyxovirus, bovine virus diarrhoea virus and transmissible gastroenteritis of pigs virus. recommendations and practical advice are given for the choice and application of chemical disinfectants for slu ... | 1995 | 7579641 |
| the drosophila melanogaster genome contains a member of the rh/t2/s-glycoprotein family of ribonuclease-encoding genes. | members of the rh/t2/s-glycoprotein family of ribonuclease(rnase)-encoding genes have been found predominantly in fungi, plants and bacteria, where they have been implicated in functions as diverse as the phosphate-starvation response and self-incompatibility. we report the isolation and sequence of dmrnase-66b, the first member of this family to be found in an insect genome. this gene was identified by the analysis of a cdna clone derived from cytological region 66b1-2 of the genome of drosophi ... | 1995 | 7607542 |
| inhibition of il-2r and sla class ii expression on stimulated lymphocytes by a suppressor activity found in homogenates of african swine fever virus infected cultures. | virus free supernatants (vfs) obtained by ultracentrifugation of homogenates of african swine fever (asf) virus infected cultures inhibited the proliferative response and the expression in peripheral blood mononuclear cells of two activation molecules, the il-2 receptor (il-2r) and the swine mhc class ii antigens (sla ii), induced by several stimuli (lectins, pma plus the calcium ionophore a23187 or specific antigen). this inhibition was time dependent: no effect was seen on il-2r expression whe ... | 1995 | 7611879 |
| vectors for the genetic manipulation of african swine fever virus. | plasmid vectors designed to facilitate the genetic manipulation of african swine fever virus (asfv) are described. our results demonstrate that the beta-glucuronidase enzyme (gus) can be used to follow gene expression in asfv-infected cells. infectious plaques formed by asfv expressing gus are visually detectable, thus providing a simple and highly sensitive method for the selection of asfv recombinants. these and previous results have allowed us to construct two chimeric gene cassettes that con ... | 1995 | 7612241 |
| production and purification of recombinant african swine fever virus attachment protein p12. | the conditions for cultivation of spodoptera frugiperda (sf9) insect cells for production of recombinant baculoviruses have been studied, to scale-up and improve the efficiency of the process for production of the african swine fever virus attachment protein p12 in the baculovirus expression system. it was shown that the total virus and recombinant protein production in insect cells infected with the acp12 recombinant baculovirus were slightly dependent on cell density, but largely dependent on ... | 1995 | 7612242 |
| isolation and characterization of tk-deficient mutants of african swine fever virus. | african swine fever virus induces the synthesis of thymidine kinase (tk) in bhk tk-negative cells as an immediate early protein. the tk gene is not essential for growth of asfv in cell culture and a stable viral strain deficient in tk has been isolated (e70ntkp). the genetic lesion of this asfv tk- strain was identified by tk gene nucleotide sequencing, showing a nucleotide deletion leading to a -1 frameshift and a nonsense codon residue downstream of the deletion. the availability of this viabl ... | 1995 | 7625127 |
| comparison of the entire nucleotide and deduced amino acid sequences of the attenuated hog cholera vaccine strain gpe- and the wild-type parental strain ald. | we have determined the complete nucleotide sequences of a live attenuated hog cholera virus (hcv) and its progenitor strain. the viral rna of each strain consisted of 12,298 nucleotides including untranslated regions of 373 and 228 bases at the 5' and 3' end, respectively. there was a single large open reading frame spanning 11,697 nucleotides which could encode a large protein of 3,899 amino acids with a calculated molecular weight of 438-kda. we have found 225 nucleotide difference between the ... | 1995 | 7661692 |
| classical swine fever virus: independent induction of protective immunity by two structural glycoproteins. | to study which proteins of classical swine fever virus (csfv) are able to confer protective immunity in swine, n-terminal autoprotease, viral core protein, and the three structural glycoproteins were expressed via vaccinia virus recombinants (vvr). csfv proteins synthesized in cells infected with vvr showed migration characteristics on sodium dodecyl sulfate gels identical to those of their respective csfv counterparts. apparently authentic dimerization of the recombinant glycoproteins was obser ... | 1995 | 7666549 |
| localization of the african swine fever virus attachment protein p12 in the virus particle by immunoelectron microscopy. | the african swine fever virus attachment protein p12 was localized in the virion by immunoelectron microscopy. purified virus particles were incubated, before or after different treatments, with p12-specific monoclonal antibody 24bb7 and labeled with protein a-colloidal gold. untreated virus particles showed labeling only in lateral protrusions that followed the external virus envelope. mild treatment of african swine fever virions with the nonionic detergent octyl-glucoside or with ethanol onto ... | 1993 | 7679861 |
| reverse interference method for measurement of hog cholera virus (hcv) and anti-hcv antibody. | a new procedure was developed for the assay of the hog cholera virus (hcv) and anti-hcv antibody. initially, the suppression effect of hcv on interferon (ifn) by hcv production was confirmed. swine kidney cell cultures preinfected with hcv produced no ifn, even following the addition of ifn inducers. however the sensitivity of the cell to ifn was not influenced by the infection with this virus. based on these results, a new method, named reverse interference method, was established. in this meth ... | 1993 | 7685639 |
| characterization of structural and non-structural proteins of hog cholera virus by means of monoclonal antibodies. | a panel of 15 monoclonal antibodies, produced against the hog cholera virus, were characterized by radioimmunoprecipitation assays. using this panel, we were able to identify 4 sets of monoclonal antibodies precipitating each a different viral protein with relative molecular weight of 40, 46, 120 kda, respectively, and a protein complex containing 15, 16, 27, and 55 kda polypeptides which were further characterized. one monoclonal antibody recognized an antigenic determinant at the c-terminal cl ... | 1993 | 7688508 |
| virulent african swine fever virus isolates are neutralized by swine immune serum and by monoclonal antibodies recognizing a 72-kda viral protein. | convalescent swine serum to african swine fever virus (asfv) isolate e75 neutralized the infectivity of virulent asfv isolates e75, e70, lisbon 60, malawi lil 20/1 and a low passage tissue culture adapted variant of e75, e75cv/v3, by 86-97% in vero and macrophage cell cultures. a monoclonal antibody, mab-135d4, recognizing an asfv protein of 72 kda also exhibited strong neutralizing activity with these viruses. unexpectedly, both e75 immune sera and mab-135d4 failed to neutralize high passage ti ... | 1993 | 7690502 |
| epitope mapping of envelope glycoprotein e1 of hog cholera virus strain brescia. | four antigenic domains (a, b, c and d) on envelope glycoprotein e1 (gp51-54) of hog cholera virus strain brescia have been specified by using 13 monoclonal antibodies (mabs) that recognize non-conserved and conserved epitopes. it was shown that the non-conserved epitopes map to the n-terminal half of e1 by analysis of chimeric e1 proteins of strains brescia and c. conserved epitopes, however, could not be mapped using this approach. here we describe mapping of both conserved and non-conserved ep ... | 1993 | 7691986 |
| [lice and methods of control]. | the morphology and biology of sucking lice (anoplura) and biting lice (mallophaga) are described. a table shows the main species for given hosts and provides simplified keys for identification. lice have a direct pathogenic effect (damage to skin and cutaneous appendages, fall in productivity) and an indirect effect (transmission of rickettsia prowazeki, r. quintana and borrelia recurrentis in human beings; african and classical swine fever virus, equine infectious anaemia virus and dipylidium c ... | 1994 | 7711304 |
| [glycoproteins from the african swine fever virus]. | the composition of african swine fever virus (asfv) glycoproteins was studied. isolate-specific major gp 110-140 was identified by radioimmunoprecipitation in lysate of porcine bone marrow cells infected with hemadsorbing virus strains. carbohydrates constitute about 50% of the total mass of gp 110-140. a quantitative method is proposed for identification of the serologic relationships between asfv hemadsorbing strains. a possible role of asfv glycoprotein carbohydrates in the hemadsorption, vir ... | 1994 | 7716925 |
| african swine fever virus gene j13l encodes a 25-27 kda virion protein with variable numbers of amino acid repeats. | the african swine fever virus (asfv) j13l gene encodes a 177 amino acid protein (19.0 kda) with a putative transmembrane domain between residues 32 and 52. there is a potential signal peptide cleavage site at residue 54 and several possible motifs for phosphorylation and myristylation. rabbit antisera raised against a synthetic peptide from the c terminus of the j13l orf identified proteins of 25-27 kda in cells infected with a recombinant vaccinia virus expressing the j13l orf, in asfv-infected ... | 1995 | 7730797 |
| ultrastructural study of the renal tubular system in acute experimental african swine fever: virus replication in glomerular mesangial cells and in the collecting ducts. | despite the considerable attention given to kidney lesions in african swine fever (asf), a number of questions remain to be answered. structural and ultrastructural examination showed that a highly virulent isolate of asf virus (malawi 83) replicated in glomerular mesangial cells and renal collecting duct epithelial cells, with hyperplasia of the latter in infected pigs. replication in mesangial cells may be due to their contact with the bloodstream, as well as to their phagocytic capacity and h ... | 1995 | 7733828 |
| a proposed division of the pestivirus genus using monoclonal antibodies, supported by cross-neutralisation assays and genetic sequencing. | sixty-six pestiviruses from ruminant and porcine hosts were analysed with a panel of 76 monoclonal antibodies raised against 9 different viruses. reactivity was used to construct epitope similarity maps for all of the viruses. four principal virus subgroups were demonstrated. one subgroup equated to classical swine fever virus (csfv) and included most porcine pestiviruses but none from ruminants. a second subgroup contained mainly viruses of bovine origin, including reference bovine viral diarrh ... | 1995 | 7735309 |
| the role of fibrinolysis in the pathogenesis of the haemorrhagic syndrome produced by virulent isolates of african swine fever virus. | the activity of several proteins involved in fibrinolysis and the morphological changes in the blood vessel walls of pigs infected with highly virulent (malawi'83) and moderately virulent (dominican republic '78-dr'78) asf virus isolates were determined. pigs infected with the malawi'83 virus developed an increased fibrinolytic activity due to high plasma levels of tissue-plasminogen activator (t-pa) of 71.3 +/- 22.8 iu/ml (mean +/- sd), which correlated well with an increased activation of inte ... | 1995 | 7740481 |
| cytopathogenicity of classical swine fever virus caused by defective interfering particles. | for three independent cytopathogenic isolates of classical swine fever virus, defective rnas were found in infected cells in addition to full-length viral genomes. these rnas represent the genomes of typical defective interfering (di) particles because of strict dependence on a complementing helper virus and interference with the replication of the helper virus. analysis of the di genomes revealed internal deletions of 4,764 nucleotides encompassing the complete structural protein-coding region ... | 1995 | 7745717 |
| further characterization of border disease virus isolates: evidence for the presence of more than three species within the genus pestivirus. | the molecular analysis of three ovine pestivirus strains revealed the existence of two distinct groups of sheep-derived pestiviruses, namely "true" border disease virus strains (bdv) and bovine viral diarrhea virus (bvdv)-like strains. as an extension of these studies rt-pcr and nucleotide sequencing of the autoprotease (npro) and nucleocapsid protein (c) encoding regions of additional serologically defined ovine pestivirus strains were performed. a comparison of npro and c revealed that three o ... | 1995 | 7747470 |
| promoter analysis of an african swine fever virus gene encoding a putative elongation factor. | 1995 | 7758704 | |
| highly specific confirmatory western blot test for african swine fever virus antibody detection using the recombinant virus protein p54. | a western blot technique using a recombinant protein has been developed to confirm positive results obtained in african swine fever (asf)-specific antibody detection by elisa. the new confirmatory western blot is based on the use of protein p54, one of the most antigenic asf virus structural proteins, expressed in escherichia coli fused to the n-terminus of ms2 polymerase. the recombinant western blot assay was highly specific and equally sensitive for asf virus-infected pigs detection as the co ... | 1995 | 7769024 |
| assessment of safety and protective value of a cell culture modified strain "c" vaccine of hog cholera/classical swine fever virus. | the protective value of a commercial strain "c" vaccine of classical swine fever (csf) was tested in weaner pigs. vaccinated animals were challenged intranasally with the virulent hog cholera virus (hcv) strain alfort/187 in groups of four pigs each at one to four weeks post vaccination, respectively. non-vaccinated control animals were challenged in the same manner. some vaccinated pigs seroconverted as early as one week post vaccination with all pigs yielding neutralizing antibodies (nab) agai ... | 1995 | 7779071 |
| a comparison of three avidin-biotin complex immunoenzyme systems for detection of african swine fever virus antigen in paraffin-embedded tissues. | the sensitivity and specificity of 3 avidin-biotin complex (abc) immunostaining systems were compared on paraffin-embedded tissues from african swine fever virus (asfv)-infected pigs. results were also compared with immunofluorescent detection on cryosections of the same tissue for optimal detection of asfv antigen. the abc-alkaline phosphatase (abc-ap) and abc-peroxidase (abc-po) systems were at least as sensitive as direct fluorescent antibody (fa) and 10-fold more sensitive than the abc-gluco ... | 1995 | 7779959 |
| early infection of interdigitating dendritic cells in the pig lymph node with african swine fever viruses of high and low virulence: immunohistochemical and ultrastructural studies. | the role of interdigitating dendritic cells (idcs) in the early pathogenesis of african swine fever (asf) was investigated using mandibular lymphoid tissue from normal pigs and pigs inoculated oronasally with highly virulent lisbon 60 (l-60) and moderately virulent dominican republic 1979 (dr-2) asf virus (asfv) isolates. paraffin-embedded tissue sections were immunostained for asfv antigen and s-100 protein, a marker of idcs, using an avidin-biotin alkaline phosphatase procedure. swine idcs wer ... | 1995 | 7779960 |
| african swine fever interference with foot-and-mouth disease infection and seroconversion in pigs. | initial oral infection of pigs with either highly virulent (l-60) or moderately virulent (dr-2) african swine fever virus (asfv), followed in 3 days with exposure to foot-and-mouth disease virus (fmdv) (tongue inoculation and contact), failed to cause fmdv infection or seroconversion in 18 of 22 l-60-infected pigs and 13 of 34 dr-2-infected pigs. of the 13 dr-2-infected pigs remaining free of foot-and-mouth disease (fmd), 2 pigs survived to 24 days without antibody to fmdv, despite constant cont ... | 1995 | 7779962 |
| african swine fever virus infection of skin-derived dendritic cells in vitro causes interference with subsequent foot-and-mouth disease virus infection. | highly purified skin-derived dendritic cells (sddcs) isolated from swine skin by a simple novel method were cultured for 24 hours before independent or sequential inoculation with african swine fever virus (asfv) and foot-and-mouth disease virus (fmdv). by avidin-biotin immunohistochemical staining, asfv antigen was detected in 50% of sddcs as early as 1.5 hours postinfection (hpi) and in 80% by 3 hpi when cytopathic effect was noted. cell lysis was detected with fmdv infection as early as 8 hpi ... | 1995 | 7779963 |
| [isolation of the agent of european swine plague from imported frozen wild boar meat]. | since july 1993 imported frozen meat of wild boars has to be screened for the presence of hcv. the number of taken samples is given by the ministry of health, sport and consumer protection. until august 1994 the total number of 688 samples from different countries, have been examined. three of them were found positive for hcv. the first one (november 1993) was from china, the other two positive samples were sent in one delivery from romania in may 1994. | 1995 | 7781542 |
| role of viral proteins and concanavalin a in in vitro replication of pseudorabies virus in porcine peripheral blood mononuclear cells. | we examined the capability of pseudorabies virus (prv) to replicate in vitro in porcine peripheral blood mononuclear cells (pbmc) and characterized the phenotype of infected cells. in addition, we investigated whether inactivation of various prv proteins or the expression of a foreign gene affected this replication. finally, we studied the replication of prv strains in concanavalin a (con a)-stimulated lymphocytes. the replication of prv mutants with inactivated glycoproteins ge or gg, thymidine ... | 1995 | 7782771 |
| genetic recombination of pseudorabies virus: evidence that homologous recombination between insert sequences is less frequent than between autologous sequences. | we studied in vivo recombination between a thymidine kinase (tk) negative, glycoprotein e (ge) negative, attenuated strain and a virulent strain of pseudorabies virus (prv) in pigs. to simplify the detection of recombination we inserted different but overlapping (375 bp) parts of the e1 gene of classical swine fever virus into the gg locus of both virus strains. recombination between the e1 sequences of these viruses results in reconstitution of the complete e1 coding sequence and expression of ... | 1995 | 7794111 |
| congenital infection of pigs with ruminant-type pestiviruses. | congenital infections of pigs were induced with two ruminant-type pestiviruses isolated from pigs. one of the viruses was bovine viral diarrhoea virus-like and the other border disease virus-like. both produced symptoms similar to those observed with low virulence strains of classical swine fever virus. a striking effect of persistent virus infection in post-natal life was stunting in viraemic animals. it was also shown that a congenitally infected pig shed virus for 2.5 years and in sufficient ... | 1994 | 7806701 |
| reverse transcriptase-pcr assay for detection of hog cholera virus. | a reverse transcriptase-pcr strategy was developed for the detection of hog cholera virus. hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. restriction endonuclease analysis identified samples as historic or recent isolates. | 1994 | 7814509 |
| comparison of pestivirus multiplication in cells of different species. | twenty-four pestiviruses, comprising 11 from pigs, eight from cattle and five from sheep, were tested for their ability to replicate in cells of porcine, bovine and ovine origin. seven of the viruses were successfully passaged in all three cell types. four porcine isolates (hog cholera virus) replicated to significant titres only in porcine cells. one bovine virus grew well in bovine cells but only poorly in ovine cells and not at all in porcine cells. the remaining 12 viruses could replicate in ... | 1994 | 7817008 |
| characterization of a ubiquitinated protein which is externally located in african swine fever virions. | an antiserum was raised against the african swine fever virus (asfv)-encoded ubiquitin-conjugating enzyme (ubcv1) and used to demonstrate by western blotting (immunoblotting) and immunofluorescence that the enzyme is present in purified extracellular virions, is expressed both early and late after infection of cells with asfv, and is cytoplasmically located. antiubiquitin serum was used to identify novel ubiquitin conjugates present during asfv infections. this antiserum stained virus factories ... | 1995 | 7853518 |
| porcine immune responses to african swine fever virus (asfv) infection. | immune responses mediating protection against asfv are poorly understood. anti-asfv antibodies may influence the course of the clinical disease but they have never been found to neutralize the virus. recent developments on cellular defense mechanisms, using swine protection models, and on the induction and role of some cytokines warrant further investigation on these areas. | 1994 | 7856069 |
| mapping and sequence of the gene encoding protein p17, a major african swine fever virus structural protein. | the gene encoding protein p17, a major structural protein of african swine fever virus, has been mapped and sequenced. protein p17 was purified from dissociated virus by reverse-phase hplc and the amino acid sequence of a peptide obtained after digestion of protein p17 with cyanogen bromide was determined by automated edman degradation. to map the gene encoding protein p17, a mixture of 17-mer oligonucleotides based upon a part of the amino acid sequence was hybridized to cloned african swine fe ... | 1995 | 7856088 |
| high level expression of the envelope glycoprotein (gp53) of bovine viral diarrhoea virus (singer) and its potential use as diagnostic reagent. | a 1.74-kb cdna fragment containing the gp53 coding region has been cloned from bovine viral diarrhoea virus (bvdv) strain singer by reverse transcription polymerase chain reaction (rt-pcr). sequence analysis indicated that gp53 of bvdv strains singer, nadl and sd-1 shared extensive sequence homology at both the rna (85-94%) and protein (82-91%) levels. nineteen cysteine residues and five potential n-linked glycosylation sites were identified within the sequenced region, all of which were conserv ... | 1994 | 7856309 |
| comparative analysis of the 5' non-coding region of pestivirus rna detected from live virus vaccines. | comparative analysis of nucleotide sequences in the 5' non-coding region (ncr) of pestivirus rna detected from live porcine and human virus vaccines indicated that the contaminants are of bovine viral diarrhea virus (bvdv), and that there are at least three genotypes, which are distinct from hog cholera virus, among the bvdv strains. most of the nucleotide changes in variable regions of the 5' ncr were covariant, with complementary substitutions at other positions for secondary structures. the p ... | 1994 | 7865600 |
| genetic heterogeneity within the coding regions of e2 and ns3 in strains of bovine viral diarrhea virus. | we have amplified and sequenced parts of the genomes of eleven laboratory strains of bovine viral diarrhea (bvd) virus originating from north america, new zealand and europe. the cumulative nucleotide (nt) sequence heterogeneity of the amplified fragments located in the analysed region of the gene encoding the nonstructural protein ns3 (p80) was 24% as compared to 47% for e2 (gp53). the nt substitutions in the e2 region resulted in replacements in 42% of amino acid (aa) positions, while the dedu ... | 1995 | 7875587 |
| replication of transfected plasmid dna by cells infected with african swine fever virus. | recombinant plasmids containing african swine fever virus (asfv) dna fragments covering all the virus genome were transfected into infected cells in order to detect viral origins of dna replication. plasmid replication was monitored by sensitivity to mboi, which cleaves only replicated, unmethylated dna, and resistance to dpni, which cleaves only the same methylated sequence. all the recombinants replicated to a similar extent, indicating that asfv does not use a preferred origin for dna replica ... | 1995 | 7886943 |
| unusual folding regions and ribosome landing pad within hepatitis c virus and pestivirus rnas. | a statistically significant folding region is identified in the 5' untranslated region (5'-utr) of hepatitis c virus (hcv), bovine viral diarrhea virus and hog cholera virus. this unusual folding region (ufr) detected in hcv encompasses 199 nucleotides (nt) and coincides with the reported internal ribosome entry site or ribosome landing pad (rlp), as determined by the 5' and 3' deletions [tsukiyama-kohara et al., j. virol. 66 (1992) 1476-1483]. the rna structure predicted in the ufr of hcv consi ... | 1995 | 7890155 |
| an african swine fever virus gene with similarity to the t-lymphocyte surface antigen cd2 mediates hemadsorption. | an open reading frame, lmw8-dr, in the african swine fever virus (asfv) genome possesses striking similarity to the lymphocyte membrane antigen cd2. all characterized cd2 domains, including the amino-terminal signal sequence, igv, hinge, igc2, stalk, transmembrane, and proline-rich carboxy cytoplasmic domains, are highly conserved in the asfv gene. critical residues for the binding of the lymphocyte function-associated antigen (lfa-3) and cd59 and for t-cell activation are also partially conserv ... | 1994 | 7907198 |
| characterization and molecular basis of heterogeneity of the african swine fever virus envelope protein p54. | it has been reported that the propagation of african swine fever virus (asfv) in cell culture generates viral subpopulations differing in protein p54 (c. alcaraz, a. brun, f. ruiz-gonzalvo, and j. m. escribano, virus res. 23:173-182, 1992). a recombinant bacteriophage expressing a 328-bp fragment of the p54 gene was selected in a lambda phage expression library of asfv genomic fragments by immunoscreening with antibodies against p54 protein. the sequence of this recombinant phage allowed the loc ... | 1994 | 7933107 |
| detection of african swine fever virus protein vp73 in tissues of experimentally and naturally infected pigs. | 1994 | 7948208 | |
| [postmortem findings in swine: non-selected submissions from hog cholera protection areas of 1992 versus selected submissions of 1991-1992]. | this article presents a survey of death-causes of all spontaneously died pigs, n = 851, from a restricted area in the province of south holland during a 2 1/2 months lasting hog-cholera epizoötic in 1992. 23 pigs from 5 submissions showed a positive ift against hog-cholera virus. those animals and pigs from sero-positive farms were excluded from this survey. the results of the post-mortems were compared with the post-mortem findings of the normally submitted, selected, animals in 1991 and 1992, ... | 1994 | 7974450 |
| segregation of bovine viral diarrhea virus into genotypes. | isolates of bovine viral diarrhea virus (bvdv) were segregated into two groups based on comparison of sequences from the 5' untranslated region (utr) of the viral genome. phylogenic analysis suggested that these groups, termed bvdv i and bvdv ii, are as different from each other as reference bvdv (bvdv-nadl, bvdv-sd-1, bvdv-osloss) are from hog cholera virus. polymerase chain reaction (pcr) tests, based on the 5' untranslated region and the genomic region coding for the p125 polypeptide, were de ... | 1994 | 7975238 |
| border disease virus: delineation by monoclonal antibodies. | many ovine pestiviruses from britain and a number of atypical porcine isolates are largely unrecognised by monoclonal antibodies (mabs) specific for reference strains of classical swine fever virus and bovine viral diarrhoea virus (bvdv). additional mabs have therefore been produced using some of these "unreactive" pestiviruses. two of the viruses used were atypical porcine isolates (strains 87/6 and vosges), whilst another had been isolated from a sheep (59386). thirty-three mabs were selected, ... | 1994 | 7979965 |
| nucleotide sequence analysis of the structural gene coding region of the pestivirus border disease virus. | border disease virus (bdv) of sheep, an important ovine pathogen, is serologically related to the two other well characterized members of the pestivirus genus of the flaviviridae family, namely bovine viral diarrhea virus (bvdv) and hog cholera virus (hocv). to determine its genetic relationship to bvdv and hocv, the genome of bdv strain, bd-78 encompassing the 5' untranslated region (utr) and structural gene coding region was molecularly cloned and the nucleotide sequence determined. the sequen ... | 1994 | 7985409 |
| detection of a cell line contaminated with hog cholera virus. | cell lines from the repository of the american type culture collection were examined for possible contamination with bovine viral diarrhea virus. during testing, hog cholera virus (hcv) was detected in the ib-rs-2 d10 porcine kidney cell line. this variant of hcv was avirulent for pigs and seldom induced detectable concentrations of antibody against reference viruses (hcv-ames or bovine viral diarrhea virus-ny1) in serum of inoculated pigs. additionally, this variant of hcv did not confer protec ... | 1994 | 7989247 |
| classical swine fever: genetic detection and analysis of differences between virus isolates. | two pairs of oligonucleotide primers were designed that specifically amplified regions of the classical swine fever virus genome. these products, corresponding to a 671 bp portion of the genes encoding the e1 and e2 (gp33 and gp55) proteins and a 1090 bp portion of the putative polymerase gene, were amplified from eight virus isolates which had been responsible for a series of classical swine fever outbreaks in italy involving both domestic pigs and wild boar. for each virus the fragments were p ... | 1994 | 7996138 |
| insect iridescent virus type 6 encodes a polypeptide related to the largest subunit of eukaryotic rna polymerase ii. | cytoplasmic dna viruses encode a dna-dependent rna polymerase (ddrp) that is essential for transcription of viral genes. the amino acid sequences of known large subunits of ddrps contain highly conserved regions. oligonucleotide primers, deduced from two conserved domains [rqp(t/s)lh and nadfdgde] were used in pcr experiments for the detection of the corresponding gene of the genome of insect iridescent virus type 6, also known as chilo iridescent virus (civ). a specific dna product of about 150 ... | 1994 | 8021587 |
| nucleotide sequence of a 55 kbp region from the right end of the genome of a pathogenic african swine fever virus isolate (malawi lil20/1). | the nucleotide sequence of a 55098 bp region from the right end of the genome of a virulent african swine fever virus (asfv) isolate (malawi lil20/1) has been determined. translation of the sequence identified 67 major open reading frames (orfs) which are closely spaced and read from both dna strands. at six positions intergenic tandem repeat arrays are found. comparison of the predicted amino acid sequences of encoded proteins with protein sequence databases identified a number of homologies. t ... | 1994 | 8021596 |
| two novel multigene families, 530 and 300, in the terminal variable regions of african swine fever virus genome. | here, we describe two novel multigene families (mgf) present in the terminal variable regions of the african swine fever virus (asfv) genome. mgf530 includes at least six related orfs (averaging 530 amino acids) containing four well-conserved domains and amino acid identities ranging from 24 to 55%. mgf300 is composed of three orfs (averaging 300 amino acids) containing three highly conserved domains and amino acid identities ranging from 25 to 46%. amino terminal regions of predicted mgf530 and ... | 1994 | 8030263 |
| nucleotide sequence of the telomeric region of the african swine fever virus genome. | the 4-kb terminal bcli fragment of african swine fever virus (asfv) dna, including the inverted terminal repetition (itr), was cloned and sequenced. the sequence showed that the 2.1-kb itr is composed of a 301-nucleotide-long unique sequence immediately adjacent to the terminal hairpin loop, a set of 38 tandem direct repeats of a 34-nucleotide degenerate sequence, another unique intervening sequence with no remarkable features, and a set of 5 tandem repeats of a 27-nucleotide unit unrelated in s ... | 1994 | 8030278 |
| [bovine virus diarrhea/mucosal disease: a review]. | infections with the bovine viral diarrhea/mucosal disease virus (bvdv) are widespread and cause a variety of diseases including reproductive disorders, abortion and malformation, pneumoenteritis, thrombocytopenia and mucosal disease. together with the closely related border disease virus of sheep (bdv) and european swine fever virus (csfv), also referred to as hog cholera virus, bvdv is now classified in the genus pestivirus of the flaviviridae family. the bvdv exists in two biotypes, noncytopat ... | 1994 | 8036482 |
| swine-reconstituted scid mice as a model for african swine fever virus infection. | injection of swine peripheral blood mononuclear cells into mice with severe combined immunodeficiency (scid), resulted in the stable long-term establishment of a functional swine immune system (scid-sw). swine immunoglobulins were present in the serum of scid-sw mice and swine cells were detected in the blood as well as in lymph nodes and spleen using monoclonal antibodies raised against cell subpopulations. swine lymphocytes from reconstituted scid mice responded in vitro to specific antigens o ... | 1994 | 8046401 |
| attempted transovarial and venereal transmission of african swine fever virus by the iberian soft tick ornithodoros (pavlovskyella) marocanus (acari: ixodoidea: argasidae). | transovarial transmission experiments were conducted with three groups of ornithodoros (pavlovskyella) marocanus velu; one group consisted of 27 pairs of adults that had been fed as larvae on a pig with a viremia of 10(7.4) had50/ml of african swine fever virus (asfv). the second and third groups each consisted of 100 pairs of adults fed on a viremic pig (10(4.5) had50/ml) as adults. the first group underwent five gonotrophic cycles over a 554-d period. the second and third groups underwent thre ... | 1994 | 8057310 |
| susceptibility of nonprimate cell lines to hepatitis a virus infection. | hepatitis a virus (hav) has been adapted to grow in primate cell cultures. we investigated replication of hav in nonprimate cells by inoculating 20 cell lines from different species with the tissue culture-adapted hm175 strain. slot blot hybridization and immunofluorescence analysis revealed that hav replicated in gpe, sp 1k, and ib-rs-2 d10 cells of guinea pig, dolphin, and pig origin, respectively. studies in ib-rs-2 d10 cells were discontinued because cultures were contaminated with classical ... | 1994 | 8057483 |
| [clinical and anatomo-pathological differences in 2 strains of african swine fever virus in angola]. | african swine fever (asf) is prevalent in angola. it is caused by several strains of viruses, among which silva-porto and huambo 85. a clinical and anatomo-pathological comparative test carried out on the natural and experimental disease showed different and significant characteristics in the behaviour of these two strains. silva-porto generates clinical signs characterized by an haemorrhagic generalized diathesis more marked on the skin, organs and viscera. in addition, the anatomo-pathological ... | 1993 | 8073167 |
| chilo iridescent virus encodes a putative helicase belonging to a distinct family within the "dead/h" superfamily: implications for the evolution of large dna viruses. | the complete nucleotide sequence of the ecori dna fragment m (7099 bp; 0.310-0.345 map units) of the genome of insect iridescent virus type 6--chilo iridescent virus (civ)--was determined. a 606 codon open reading frame located in this region encoded a protein (p69) related to a distinct family of putative dna and/or rna helicases belonging to the "dead/h" superfamily. unique sequence signatures were derived that allowed selective retrieval of the putative helicases of the new family from amino ... | 1994 | 8073636 |
| african swine fever virus encodes a cd2 homolog responsible for the adhesion of erythrocytes to infected cells. | we have identified an open reading frame, ep402r, within the ecori e' fragment of the african swine fever virus genome that encodes a polypeptide of 402 amino acid residues homologous to the adhesion receptor of t cells, cd2. transcription of ep402r takes place during the late phase of virus replication. the disruption of ep402r, achieved through the replacement of a 354-bp-long fragment from within ep402r by the marker gene lacz, does not affect the virus growth rate in vitro but abrogates the ... | 1993 | 8102411 |
| virulence and pathogenesis of non-virulent and virulent strains of pseudorabies virus expressing envelope glycoprotein e1 of hog cholera virus. | pseudorabies virus (prv) expressing the envelope glycoprotein e1 (e1) of hog cholera virus (hcv) was used as a model to study the potential risks connected with the use of a live herpesvirus vaccine expressing a foreign gene. the gene encoding e1 was inserted into the glycoprotein x (gx) locus of both a virulent prv strain and a non-virulent prv strain in which the virulence genes encoding glycoprotein i (gi) and thymidine kinase (tk) had been inactivated. we investigated whether strain m205 (gi ... | 1994 | 8113720 |
| identification of genes encoding zinc finger proteins, non-histone chromosomal hmg protein homologue, and a putative gtp phosphohydrolase in the genome of chilo iridescent virus. | five rna transcripts of about 1.2 to 1.7 kilobases were mapped to a part of the genome of insect iridescent virus type 6 (chilo iridescent virus; civ) between genome coordinates 0.832 and 0.856 within the ecori dna fragment f. the nucleotide sequence of this particular region (5702 base pairs) of the civ genome was determined. the dna sequence contains a number of perfect direct, inverted, and palindromic repeats including three clusters of tandemly organized repetitive dna elements located betw ... | 1994 | 8121799 |
| genetic identification and nucleotide sequence of the dna polymerase gene of african swine fever virus. | the dna polymerase gene of african swine fever virus (asfv) was mapped by marker rescue experiments using a phosphonoacetic acid-resistant mutant and hybridization with an oligonucleotide probe designed from the most conserved motif of family b dna polymerases. viral dna fragments mapping in this region were cloned and sequenced. an open reading frame coding for a 1244 amino acid long peptide with a molecular mass of 142.5 kda was determined from the sequence. a unique feature of asfv dna polyme ... | 1994 | 8121806 |
| [field infection with bvd virus in swine: epidemiology and diagnosis]. | in a pig breeding herd in lower saxony infertility of breeding sows had been repeatedly observed. growth retardation and post mortem findings in two piglets gave clinical indication to swine fever/hog cholera. a virus was isolated and typed by monoclonal antibodies as pestivirus not identical with hog cholera virus (hcv). in neutralization tests applying the field isolate, hcv and bovine viral diarrhea (bvd) virus the sera breeding sows and weaner pigs yielded high neutralizing antibody titres a ... | 1994 | 8131728 |
| [prevalence of antibodies against the viruses of european swine fever, aujeszky's disease and "porcine reproductive and respiratory syndrome" in wild boars in the federal states sachsen-anhalt and brandenburg]. | during the hunting season from 1991/1992 blood samples were collected from wild boar shot in the federal states of sachsen-anhalt (482 samples) and brandenburg (177 samples) which corresponds to 2.1 and 0.4% of the total hunting bag. all sera were screened in a complex trapping blocking (ctb) elisa for antibodies against hog cholera virus (hcv) and in an indirect elisa for antibodies against aujeszky's disease virus (adv). additionally the sera were tested for neutralizing antibodies against hcv ... | 1994 | 8131731 |
| multigene families in african swine fever virus: family 505. | sequencing of restriction fragment ecori a-sali c of african swine fever virus has revealed the existence of a multigene family, designated family 505 because of the average number of amino acids in the proteins, composed of seven homologous and tandemly arranged genes. all the genes of family 505 are expressed during infection. primer extension analysis showed that transcription is initiated a short distance (3 to 62 nucleotides) from the start codon of the corresponding open reading frame. the ... | 1994 | 8139051 |
| monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains. | monoclonal antibodies to bovine viral diarrhea virus (bvdv) were examined for binding with a large number of north american bvdv isolates and eight strains of the serologically related pestivirus, hog cholera virus (hcv). no single bvdv monoclonal antibody reacted with all bvdv isolates. the most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) north american isolates. from a fewer number of isolates tested, one anti-gp53 mon ... | 1994 | 8143258 |
| a rapid and sensitive chemiluminescence dot-immunobinding assay for screening hybridoma supernatants. | the present report describes a simple and rapid dot-immunobinding assay combined with a chemiluminescence detection system for screening hybridoma supernatants for specific monoclonal antibodies (mabs). small rectangular nitrocellulose filters dotted with either crude mixtures of antigens, or with control samples, were placed in six well plates, incubated with hybridoma supernatants, then stained with peroxidase-conjugated anti-mouse igg. the reaction was performed with a chemiluminescence detec ... | 1994 | 8157996 |
| epidemiology of classical swine fever in sardinia: a serological survey of wild boar and comparison with african swine fever. | a serological survey was carried out to establish the distribution of classical swine fever among wild boar in sardinia, where that disease and african swine fever have been endemic in free-ranging domestic pigs and wild boar living in the mountainous areas of the province of nuoro for several years. blood samples were collected from 4752 wild boar shot during the period december 1988 to january 1992. an overall prevalence of 11 per cent was observed and the almost constant rate of about 9.8 per ... | 1994 | 8171792 |
| glycoprotein e2 of classical swine fever virus: expression in insect cells and identification as a ribonuclease. | two regions of amino acids homologous to the ribonuclease catalysis domain of the fungal rnases t2 of aspergillus oryzae and rh of rhizopus niveus and the plant s-glycoproteins of nicotiana alata are perfectly conserved in the amino acid sequence of the envelope glycoprotein e2 of classical swine fever virus (csfv). to analyze the functional significance of these conserved sequences, the gene encoding e2 was inserted into the p10 locus of baculovirus and expressed in insect cells. recombinant vi ... | 1994 | 8178442 |
| nucleotide sequence and variability of the inverted terminal repetitions of african swine fever virus dna. | african swine fever virus (asfv) genome is a large (170-190 kb) double-stranded dna molecule with structural features similar to those of poxviruses. prominent among those features are the presence of a hairpin loop structure at the end of the dna molecule and terminal-inverted repeats (tir). the tirs have been previously demonstrated by electron microscopy and cross-hybridization of terminal restriction fragments. we have determined the sequence of both left and right dna ends from the ba71v vi ... | 1994 | 8178480 |
| application of a blocking enzyme-linked immunosorbent assay for serological monitoring of hog cholera (classical swine fever) in poland. | between 1990 and 1992, serum samples from 55,478 domestic swine were tested by enzyme-linked immunosorbent assay (elisa) for the presence of hog cholera virus (hcv) antibodies. the amount of antibody in the sera was expressed as the mean percentage inhibition (pi). for diagnosis, the tested sera were diluted 1:2 and considered positive if the pi was less than 25%. sera giving pi values in the range of 25-50% were retested against hcv and bovine virus diarrhoea virus (bvdv), by neutralising perox ... | 1993 | 8219338 |
| virus-host interactions in african swine fever: the attachment to cellular receptors. | biochemical and morphological techniques have shown that african swine fever virus (asfv) enters susceptible cells by a mechanism of receptor-mediated endocytosis. the virus binds to a specific, saturable site in the cell and this interaction is required for a productive infection. a structural asfv protein of 12kda (p12) has been identified to be involved in the recognition of the cellular receptor, on the basis of the specific binding of the polypeptide to sensitive vero cells. protein p12 is ... | 1993 | 8219802 |
| african swine fever virus genome content and variability. | a 55 kilobase pair (kb) region from the right end of the virulent african swine fever virus isolate, malawi lil20/1, has been sequenced. the 68 major open reading frames (orfs) encoded are generally closely spaced and read from both dna strands across the complete sequence. comparison of the amino acid sequences of predicted orfs with sequence databases identified 15 orfs which encode proteins that are similar to proteins of known function. two orfs are homologous to copies of multigene family 3 ... | 1993 | 8219803 |
| molecular characterization of positive-strand rna viruses: pestiviruses and the porcine reproductive and respiratory syndrome virus (prrsv). | molecular characterization has become an important tool for the analysis of viruses including their classification. the manuscript focuses on the molecular analysis of two members of the genus pestivirus (hog cholera virus, hcv and bovine viral diarrhea virus, bvdv) and of the recently discovered porcine reproductive and respiratory syndrome virus (prrsv). the first protein encoded within the single large pestivirus orf is a nonstructural protein with autoproteolytic activity. the cleavage site ... | 1993 | 8219812 |
| the relative density of cd44-positive porcine monocytic cell populations varies between isolations and upon culture and influences susceptibility to infection by african swine fever virus. | african swine fever (asf) virus has been reported to infect cells of the monocyte family, probably macrophage-like cells, but there is variation in the apparent susceptibility of these cells. we have demonstrated that the phenotype and activity of porcine monocytic cells varies between different isolations and also upon culture. the variation during culture is dependent upon the phenotype of the cells at the time of isolation. as for the susceptibility of porcine monocytes/macrophages to infecti ... | 1993 | 8225410 |
| processing of pestivirus polyprotein: cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus. | the polyprotein of classical swine fever virus starts with the nonstructural protein p23, which is followed by the nucleocapsid protein p14. proteolytic cleavage between p23 and p14 was demonstrated in a cell-free transcription-translation system. successive truncation of the cdna used for the transcription indicated that the proteolytic activity responsible for the cleavage between p23 and p14 resides within p23. in order to determine the cleavage site between these two proteins, the respective ... | 1993 | 8230432 |
| african swine fever virus interaction with microtubules. | the role of microtubules in intracellular transport of african swine fever virus (asfv) and virus-induced inclusions was studied by immunofluorescence using anti-asfv and anti-tubulin antibodies, by electron microscopy of infected vero cells and by in vitro binding of virions to purified microtubules. mtc, a reversible colchicine analogue, was used to depolymerize microtubules. in cells treated with mtc multiple large inclusions containing asfv antigens and particles were observed in the cytopla ... | 1993 | 8241964 |
| mapping and sequence of the gene encoding the african swine fever virion protein of m(r) 11500. | the gene encoding the african swine fever virus protein of m(r) 11,500, present in the virus particle, has been mapped and sequenced in the genome of the vero cell-adapted virus strain ba71v. a serum raised against virion proteins of m(r) 12,000 to 13,000 isolated from polyacrylamide gels was used to screen a plasmid expression library, containing viral dna random fragments, that expresses viral polypeptides fused to beta-galactosidase. using this method, we have identified and sequenced the ope ... | 1993 | 8245848 |
| long-term persistent infection of swine monocytes/macrophages with african swine fever virus. | long-term persistent infection was established in 100% of pigs (n = 19) experimentally infected with african swine fever virus (asfv). viral dna was detected in peripheral blood mononuclear leukocytes (pbml) at greater than 500 days postinfection by a pcr assay. infectious virus was not, however, isolated from the same pbml samples. in cell fractionation studies of pbml, monocytes/macrophages were found to harbor viral dna during the persistent phase of infection. this result indicates that mono ... | 1994 | 8254776 |
| passively transferred african swine fever virus antibodies protect swine against lethal infection. | the role of anti-viral antibodies in homologous protective immunity to a virulent african swine fever virus (asfv) strain e75 was examined by passive transfer experiments in swine. eighty-five percent of animals (n = 14) that received anti-asfv immunoglobulin (ig) survived challenge infection, while 100% mortality was observed in control group animals (n = 28) that received anti-pseudorabies virus ig, normal swine ig, or phosphate-buffered saline. with the exception of a significantly delayed an ... | 1994 | 8259670 |
| two putative african swine fever virus helicases similar to yeast 'deah' pre-mrna processing proteins and vaccinia virus atpases d11l and d6r. | two open reading frames (orfs) of african swine fever virus (asfv) encoding putative helicases have been sequenced. the two genes, termed d1133l and b962l, are located in the central region of the viral genome, but are separated by about 40 kb of dna. both genes are expressed late during asfv infection of vero cells, after replication of viral dna has begun. contiguous to d1133l, three other orfs (d129l, d79l and d339l), encoding putative proteins of unknown function, have been sequenced. protei ... | 1993 | 8262374 |
| nucleotide sequence of a nucleoside triphosphate phosphohydrolase gene from african swine fever virus. | a putative nucleoside triphosphate phosphohydrolase (ntpase) gene of african swine fever virus was identified by using a degenerate oligonucleotide probe derived from the nucleoside triphosphate binding motif, which is highly conserved among viral and cellular ntpases. the probe hybridized with fragments sali e and ecori q, which is entirely contained in the former one. sequencing of this region revealed an open reading frame, designated q706l, coding for a protein of 706 amino acids, with a cal ... | 1993 | 8266720 |
| characterization of vaccinia virus gene b12r. | we report the characterization of vaccinia virus gene b12r which is predicted to encode a 33k protein with 36% amino acid identity to the serine/threonine protein kinase encoded by vaccinia virus gene b1r. s1 nuclease protection experiments showed that gene b12r is transcribed early during infection from an initiation site 11 bp upstream of the open reading frame (orf). the gene encodes a 33k polypeptide that is not required for virus replication in tissue culture nor for virus virulence in a mu ... | 1993 | 8277291 |
| molecular characterization of border disease virus, a pestivirus from sheep. | three serologically different pestivirus strains isolated from sheep were selected for molecular analysis. cdna and deduced amino acid sequences of the genomic regions encoding glycoproteins e1 and e2 were obtained from the three strains. a comparison with amino acid sequences of bovine viral diarrhea virus (bvdv) and classical swine fever virus (csfv) revealed that one of the three ovine pestivirus strains can be grouped together with bvdv. the other two strains, however, were clearly different ... | 1994 | 8291236 |
| the dna polymerase-encoding gene of african swine fever virus: sequence and transcriptional analysis. | the putative dna polymerase-encoding gene of african swine fever virus has been sequenced. the gene, designated g1207r, is located in the central region of the viral genome, and encodes a protein of 1207 amino acids (aa) with a predicted m(r) of 139,835. the gene is transcribed at both early and late stages of infection into a 4.1-kb rna. transcription is initiated at tsp, 8 nucleotides (nt) upstream from the start codon. open reading frame (orf) g1207r contains four direct repeats in tandem clo ... | 1993 | 8293992 |
| [lipids from the african swine fever virus]. | the lipids of highly purified african swine fever virus (asfv) propagated in porcine bone marrow cells were observed to contain 25.6% phospholipids, 9.7% monoglycerides, 14.1% cholesterol, 17.8% free fatty acids, 14.4% diglycerides, 13.6% triglycerides, and 6.7% cholesterol ethers. diethyl ether extracts mono-, di-, triglycerides, free fatty acids, 50% of cholesterol and cholesterol ethers, and 25% of phospholipids from the virus. analysis of the 14c-sodiumacetate incorporation into viral, cellu ... | 1993 | 8302309 |