Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a transmissible chicken tumour associated with reticuloendotheliosis virus infection. | histiocytic lymphosarcomas of the intestine, liver, spleen and sciatic nerve were found at necropsy in a 36-week-old laying hen that was culled from a flock of 1800 birds because of emaciation. type c particles were observed in ultrathin sections of liver and spleen. the serum of the hen contained reticuloendotheliosis virus (rev) antigen, and antibody against rev, but lacked antibodies reactive with marek's disease virus or subgroups a and b of rous sarcoma virus. the tumour was transmitted to ... | 1980 | 6250524 |
| replicatin of the resident marek's disease virus genome in synchronized nonproducer mkt-1 cells. | mkt-1, a virus nonproducer lymphoblastoid cell line established from a marek's disease tumor, was synchronized by double thymidine block to determine the sequence of events in the synthesis of cellular and latent marek's disease virus dna. cellular dna synthesis was measured by incorporation of [3h]thymidine, whereas viral dna synthesis was determined by dna-dna reassociation kinetics. the results of these studies indicate that the resident marek's disease viral dna in mkt-1 cells replicates dur ... | 1980 | 6251256 |
| dna of a chicken herpesvirus is associated with at least two chromosomes in a chicken lymphoblastoid cell line. | 1980 | 6251606 | |
| [immunoprophylaxis against marek's disease and its perspectives]. | an account is given of the present international position in immunoprophylactic control of marek's disease. an assessment is made of the method used for immunisation in the gdr. in that context, reference is made to problems for which solutions will have to be found in the long run. | 1980 | 6251769 |
| performance of 3 successive generations of specified-pathogenfree chickens maintained as a closed flock. | no antibodies against salmonella pullorum, mycoplasma gallisepticum, mycoplasma synoviae, haemophilus gallinarum, fowl pox virus, marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. antibodies against i ... | 1980 | 6253742 |
| [continuous lymphoblastoid cell lines in virological research]. | 1980 | 6254262 | |
| persistence of genomes of both herpesvirus of turkeys and marek's disease virus in a chicken t-lymphoblastoid cell line. | a cell line tentatively designated as mdcc-bo1(t), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (hvt). bo1(t) cells were t lymphoblastoid cells and the more than 95% of them had marek's disease (md) tumor-associated surface antigen (matsa). however, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-hvt and -md virus (mdv) sera. the virus could be rescued from bo1 ... | 1980 | 6254498 |
| virus-viral co-cancerogenesis and the other viral interactions. | the article presents the data obtained by the authors in studies of virus-viral co-cancerogenesis, the interaction between some non-oncogenic viruses and well-known oncogenic viruses, the results of co-cancerogenic effect marek's disease herpesvirus with the avian leukemia virus and the possibility of phenotype mixing between oncornaviruses belonging to different species in nature. | 1980 | 6257199 |
| latent infections with marek's disease virus and turkey herpesvirus. | the number of plaque-forming units (pfu) from lymphocytes latently infected with oncogenic (ga-5) or nononcogenic (sb-1) marek's disease virus (mdv) was decreased after complement lysis with antisera against thymus-derived lymphocytes or bursa-derived lymphocytes; an additive effect with dual treatment was observed. complement lysis with antisera against marek's disease tumor-associated surface antigen (putative tumor antigen) had little effect, which suggests an absence of the antigen on latent ... | 1981 | 6259403 |
| activation of the alternative complement pathway by normal and transformed cells in homologous and heterologous sera. | a variety of both normal and viral transformed or infected cells have been tested for their ability to activate the alternative pathway of complement in either homologous or heterologous sera. normal cell lines fail to produce alternative pathway activation in homologous sera but readily activate the alternative pathway in heterologous sera. in contrast, human cells, persistently infected with measles virus or chicken cells transformed with oncogenic avian viruses, produce a marked activation in ... | 1981 | 6260511 |
| influence of the bursa of fabricius on the pathogenesis of marek's disease. | a series of experiments was conducted to study the influence of embryonal bursectomy (ebx) on the early and late pathogenesis of marek's disease (md). the early lytic infection in the lymphoid organs normally associated with oncogenic md virus infection in intact chickens was not seen in ebx chickens. therefore, the damage to the immune system was minimal. ebx chickens also had lower viremia levels, higher lymphocyte responses to mitogens, and a lower or delayed md mortality when compared with i ... | 1981 | 6260660 |
| comparative 50% protective dose assays (pd50) of marek's disease virus strain cvi988. | 1981 | 6260810 | |
| [evidence for the persistence of epstein-barr-virus in the parotid gland (author's transl)]. | ebv is associated with b-lymphocytes. it is, however, not clear whether spontaneous activation of ebv genomes in carrier lymphocytes, which are present in the lymphocyte rich area of the oropharynx, is responsible for lifelong persistence of antibody-titers directed against ebv related antigens and the shedding of virus into the oropharynx. alternatively ebv could reside in specific sites of the body and be produced there resembling somehow the situation of marek's disease virus of chicken. that ... | 1981 | 6261052 |
| nature of genetic resistance to marek's disease in chickens. | infection of line 6 resistant and line 7 susceptible chickens with marek's disease virus (mdv) resulted in a depressed phytohemagglutinin (pha) response and the presence of marek's disease (md) tumor-associated surface antigen (matsa) in the spleens. at 6-10 weeks after infection, recovery in pha response, diminution in the number of matsa cells, and the presence of significant anti-matsa immunity were observed in line 6 but not in line 7 chickens. both lines had antibody-dependent cell-mediated ... | 1981 | 6262555 |
| [study of viral contamination of japanese quail and cell cultures from their embryos]. | agents identified as adenovirus celo were isolated from organ suspensions of 1500 japanese quails (jq) in 4 experiments. no contaminating viruses were found in examinations of cell cultures prepared from the kidneys of 80 jq. negative results were obtained in examinations of 163 antigens from jq organs in the cofal test. agents identified as mycoplasma were isolated in 16 cases from 736 specimens of the virus-containing fluid used for manufacture of measles virus. according to the results of the ... | 1981 | 6266156 |
| attempts ot activate endogenous virus expression with marek's disease virus dna. | several dnas derived from marek's disease virus-infected cells and tissues were tested for in vitro infectivity and for the ability to activate avian endogenous type c virus. the dna isolated from tumour tissue, peripheral blood buffy coat cells, mdv-infected tissue cultures, lymphoblastoid cell lines and feather follicle epithelium cells from mdv-infected birds elicited a negative response in transfection assays. the mdv dnas isolated did not activate the endogenous type c virus from cell cultu ... | 1981 | 6266886 |
| expression of forssman antigen of avian lymphoblastoid cell lines transformed by marek's disease virus or avian leukosis virus. | the expression of forssman-type heterophile antigen on marek's disease (md) virus (mdv)-transformed cell lines, mdcc-msbi 1, -hp1, -rp1 and -bp1, and avian leukosis virus (alv)-transformed cell lines, lscc-1104b1 and -1104x5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. forssman antigen was detected on a high percentage of the cells in two alv-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. of ... | 1981 | 6267170 |
| immune response and disease resistance in chickens. i. selection for high and low titer to salmonella pullorum antigen. | a selection experiment for high and low anti-salmonella pullorum antibody titer was carried out over four generations within the b1b1 blood group genotype in chickens. the study was aimed primarily at identifying different response patterns controlled by immune response genes linked to the b system, the major histocompatibility complex of the chicken. maximal divergence was obtained in the third generation of selection when agglutination titers of 1/320 and 1/80 in b1b1 high and low responders, ... | 1981 | 6267577 |
| serological responses to mycoplasma synoviae in chickens infected with virulent or avirulent strains of marek's disease virus. | the pathogenicity and immunosuppressive capability of five strains of marek's disease herpesvirus (mdhv) was examined. three of the strains (ala, miss, ncs) were isolated from flocks experiencing vaccine breaks while the other two strains (ga, hn) represented prototype virulent and avirulent viruses. pathogenicity of mdhv strains was evaluated on their ability to cause mortality, their ability to cause gross or microscopic lesions, and the median latent period to death (mlpd) for each strain. an ... | 1981 | 6267580 |
| fractionation of marek's disease virus-induced lymphoma by velocity sedimentation and association of infectivity with cellular fractions with and without tumor antigen expression. | cell suspensions of lymphomas induced by marek's disease (md) virus were fractionated by sedimentation at unit gravity on a continuous gradient of bovine fetal serum. cells in various fractions were examined for md tumor-associated surface antigen (matsa) by indirect immunofluorescence, using specific antibody, and for viral infectivity by cocultivating fractionated cells with permissive monolayer cells of duck embryo fibroblasts. most matsa-bearing cells in the lymphomas sedimented at a sedimen ... | 1981 | 6267963 |
| effects of infectious bursal disease on marek's disease vaccination: suppression of antiviral immune response. | studies were made to determine whether infectious bursal disease virus (ibdv) infection would affect the response of chickens to turkey herpesvirus (hvt) vaccination in the development and level of hvt viremia and virus-neutralizing (vn) antibodies to hvt. the hvt viremia in the vaccinated chickens was not affected by ibdv, whether ibdv was inoculated simultaneously with hvt vaccination at one day of age or whether it was inoculated 3 weeks postvaccination with hvt. however, vn antibody response ... | 1980 | 6268042 |
| difference between influences of homologous and heterologous maternal antibodies on response to serotype-2 and serotype-3 marek's disease vaccines. | marek's disease (md) vaccines representing serotypes 2 (sb-1 strain of md virus) and 3 (fc-126 strain of turkey herpesvirus) were administered to 1-day-old md-susceptible chicks that either were free of antibodies or carried maternally derived antibodies against sb-1, fc-126, or a serotype-1 md virus, cu-2 strain. homologous antibodies delayed the development of vaccine virus viremias and inhibited vaccinal immunity, as judged by protection against challenge with the virulent jm-10 strain of md ... | 1981 | 6268052 |
| latency of herpesvirus of turkey and marek's disease virus genomes in a chicken t-lymphoblastoid cell line. | the properties of latent herpesvirus of turkey (hvt) and marek's disease virus (mdv) genomes have been studied in virus-non-producer mdcc-bo1(t) cells, a t-lymphoblastoid cell line derived from spleen cells of an hvt-vaccinated chicken. the numbers of the two virus genomes in bo1(t) cells remained stable at 1.6 to 1.8 hvt genome equivalents/cell and 3.4 to 3.8 mdv genome equivalents/cell throughout a number of passages and were not decreased by the presence of phosphonoacetic acid in the culture ... | 1981 | 6268738 |
| restriction endonuclease analysis of the genomes of virulent and avirulent marek's disease viruses. | the dnas from virulent strain bc-1 and avirulent strains c2(a) of marek's disease virus (mdv) were compared by electrophoresis on 0.5% agarose gels of the products obtained with the restriction endonucleases bam h1, sal 1, and sma 1. the patterns of the fragments of the dnas from these two strains were very similar, but showed some significant differences in the number and mobility of the dna bands. the dna fragments obtained with the restriction endonucleases, and especially sma 1, were mostly ... | 1981 | 6268948 |
| immunoprecipitation of marek's disease virus-specific polypeptides with chicken antibodies purified by affinity chromatography. | 1981 | 6269290 | |
| mice inoculated with marek's disease tumor cells: increased number of lymphomas. | 1981 | 6269799 | |
| genetic resistance to herpesvirus-induced lymphoma at the level of the target cell determined by the thymic microenvironment. | 1981 | 6270018 | |
| herpesvirus infection enhances cholesterol and cholesteryl ester accumulation in cultured arterial smooth muscle cells. | in our previous experiments, atherosclerosis similar to that in humans was reproducibly induced in both normocholesterolemic and hypercholesterolemic specific-pathogen-free (spf) chickens by infection with marek's disease herpesvirus (mdv). in contrast, uninfected chickens fed either relatively cholesterol-poor or cholesterol-supplemented diets did not develop this arterial disease. in experiments reported here, the hypothesis that infection of arterial smooth muscle cells (smcs) with mdv would ... | 1981 | 6271018 |
| herpsevirus-induced atherosclerosis. | 1981 | 6271616 | |
| spontaneous and induced herpesvirus genome expression in marek's disease tumor cell lines. | we incubated 31 newly established marek's disease tumor cell lines at 41 degrees c for 48 h after subculturing and then examined them to determine the spontaneous rates of expression of viral internal antigen(s), viral membrane antigen(s), and virus isolation. all but two of the lines were isolated from tumors induced by clone-purified marek's disease virus strain jm-10, ga-5, rb-1b, and bc-1a in nine different genetic strains of chickens with defined histocompatibility antigens. the line-to-lin ... | 1981 | 6273319 |
| structural changes in the dna of marek's disease virus during serial passage in cultured cells. | 1981 | 6274089 | |
| persistence and expression of marek's disease virus dna in tumour cells and peripheral nerves studied by in situ hybridization. | we have used cloned fragments of marek's disease virus (mdv) dna and in situ hybridization to search for virus dna and study its expression in infected chick embryo fibroblasts (cef), lymphoblastoid cell lines, tumours and neural lesions. dna from the hprs 16/att strain of mdv was cleaved with ecori endonuclease and several fragments were cloned in escherichia coli using the vector pbr322. seven fragments ranging in size from 2.6 to 11 kbp representing approx. 25% of the mdv genome were labelled ... | 1981 | 6275026 |
| detection of normal and aberrant chromosomes in chicken embryos and in tumor cells. | 1981 | 6275374 | |
| [transmission of marek's disease to wild feathered game]. | the following wild feathered game were tested from 6 to 12 months of age: common pheasant (phasianus colchicus), common gray partridge (perdix perdix), european quail (coturnix coturnix), and greek partridge (alectoris graeca). only pheasant was found to be susceptible to infection with a virulent virus of marek's disease. in this bird the first clinical symptoms of the disease (nervous changes) were observed in 28 days from infection. immunoprecipitation antibodies to the marek's disease virus ... | 1981 | 6275596 |
| establishment of b-lymphoblastoid cell lines from marek's disease virus-induced tumors in turkeys. | four lymphoblastoid cell lines were established from tumours of turkeys inoculated with high doses of the ga strain of marek's disease virus (mdv). unlike other md lymphoblastoid cell lines of chicken origin, these mdv transformed turkey line appear to be b lymphocytes and produce immunoglobulin. growth characteristics of these cell lines are slightly different; however, they all produce low levels of mdv-specific antigen and carry the complete genome of the virus as determined by virus rescue i ... | 1982 | 6277804 |
| the mechanism of genetic resistance to marek's disease in chickens. | genetic resistance to marek's disease in rpl line-6 chickens is expressed not only at the level of host immunological responses against virus an tumour antigens, but also at the level of target lymphoid cells for virus infection and transformation. the nature of the target cell involved was investigated. spleen cells from susceptible line-7 chickens adsorbed more marek's disease virus and turkey herpesvirus in vitro than line-6 spleen cells. in the case of marek's disease virus this was reflecte ... | 1982 | 6277808 |
| 1980 p. p. levine award (dr. r. l. witter). | 1981 | 6279067 | |
| a simple in vitro differentiation between turkey herpesvirus and marek's disease virus. | the growth and plaque formation by turkey herpesvirus (hvt) amd marek's disease herpesvirus (mdhv) were examined in qt35 cells, a continuous fibroblast cell line derived from chemically induced tumors of japanese quail. hvt grew and formed plaques consistently in qt35 cells when inoculated with cell-culture-propagated virus or peripheral mononuclear leukocytes (pml) from chickens that had been inoculated with hvt. both oncogenic and nononcogenic strains of mdhv, however, failed to grow and induc ... | 1981 | 6279072 |
| natural killer cell activity in chickens exposed to marek's disease virus: inhibition of activity in susceptible chickens and enhancement of activity in resistant and vaccinated chickens. | chickens of 2 genetic lines (lines p and n) were inoculated with a pathogenic strain of marek's disease (md) virus (mdv) and chronologically examined for disease response and natural killer (nk) cell expression. the nk cell reactivity was assayed in an in vitro cytotoxicity assay in which effector cells from the spleen of test chickens were reacted with 51cr-labeled lscc-rp9 target cells. chickens of line p developed progressive debilitating disease and a high incidence of gross tumors and death ... | 1981 | 6279075 |
| induction of lymphomas and tumor antigen by marek's disease virus in turkeys. | pathogenic marek's disease (md) virus (mdv) induced a high incidence of mortality and gross and microscopic lesions in turkey poults. inoculated turkey poults became persistently viremic with mdv, although the levels of detectable circulating mdv were generally lower in turkeys than in similarly inoculated chickens. the early lytic phase of md characterized by lymphoid cell destruction and the appearance of viral antigen in lymphoid organ was not as prominent in turkeys as in chickens. gross md ... | 1981 | 6279077 |
| marek's disease in turkeys: lack of protection by vaccination. | herpesvirus of turkeys, a highly effective vaccine against marek's disease (md) in chickens, was ineffective in protecting turkeys against md. another tissue-culture attenuated vaccine virus also protected chickens, but not turkeys, from md. intact and immunosuppressed turkey poults inoculated with herpesvirus of turkey developed a persistent viremia, but did not have detectable gross or microscopic lesions. | 1982 | 6280536 |
| inverted repeat nucleotide sequences in the genomes of marek disease virus and the herpesvirus of the turkey. | the dnas of two herpesvirus, the oncogenic marek disease virus and the serologically related herpesvirus of the turkey, were studied by electron microscopy. on the basis of fold-back molecules observed in single-stranded dna from both viruses, structures have been derived from the overall nucleotide sequence arrangement in their genomes. although differing in molecular weight, the genomes of marek disease virus and turkey herpesvirus are both constructed according to the same plan--two regions o ... | 1982 | 6281774 |
| influence of oncogenicity of marek' disease virus on evaluation of genetic resistance. | resistance to marek's disease ( md) is, in part, genetically determined and linked to the major histocompatability complex. the genetic resistance of 9 previously characterized lines of chickens and also of the inbred ucd-003 and 4 congenic lines was evaluated by challenge with jm-10. five lines were highly resistant. these included the n-line and two derivatives from n-line (n-2 and n-5), pdrc strain, and one of the congenic ucd lines. the pdrc genotype is unknown, but the 3 related lines carry ... | 1981 | 6283507 |
| [resistance of broilers to marek's disease virus]. | studied was the resistance, resp., susceptibility of the initial broiler lines - 66 and 77 of the cornish breed, and 88 and 99 of the plymouth rhock breed, which are used in this country for the production of the four-line hybrid, - to the virus of the marek's disease, isolate ct-1. a biologic assay was carried out with a total of 2080 young birds divided into three groups; i groups - infected abdominally; ii group - contacts; and iii group - controls. certain breed, line, and sex differences we ... | 1981 | 6283725 |
| the immune response of the chick following viral vaccinations and immunization with sheep red blood cells. | the effects of viral vaccinations and immunization with sheep red blood cells (srbc) on the humoral response of pullets were investigated. pullets were vaccinated with marek's disease virus, newcastle disease virus (ndv), infectious bronchitis virus (ibv), and infectious bursal disease virus at appropriate ages used in commercial practice. at seven weeks, the pullets were intramuscularly immunized with srbc. ndv and ibv antibodies were detected by hemagglutination-inhibition tests. hemagglutinat ... | 1982 | 6284114 |
| collaborative study of the assay for virus content of marek's disease vaccines using a reference preparation. | 1982 | 6284759 | |
| dose-dependent inhibition of virus rescue from lymphocytes latently infected with turkey herpesvirus or marek's disease virus. | the number of plaque-forming units (pfu) of turkey herpesvirus (hvt) isolated per 10(6) latently infected splenic lymphocytes was determined by co-cultivation on permissive monolayer cultures in 35-mm-diameter petri dishes. doses of 1 x 10(6) spleen cells or less per culture gave uniform dose-related titers, whereas doses of 8 x 10(6) cells often yielded less than 1-2% of the expected number of pfu. intermediate doses gave proportionally reduced virus yields. this dose-dependent inhibition was o ... | 1982 | 6285880 |
| effect of t & b cell immunosuppression on the release of marek's disease virus from the feather follicles of chickens. | 1982 | 6286472 | |
| characterization of an antigen associated with the marek's disease lymphoblastoid cell line msb-1. | a marek's disease lymphoblastoid cell line (msb-1) has been analysed by immunoprecipitation for expression of tumour-associated antigen, marek's disease virus (mdv)-specific antigens and antigens specific to avian leukosis-sarcoma viruses. rabbit antisera raised against two independently derived cell lines after extensive absorption with normal chick cells reacted with a polypeptide of mol. wt. 40 000 (40k) in extracts of msb-1 cells. the 40k polypeptide was not present in myeloblasts or in chic ... | 1982 | 6286857 |
| in vitro infection of lymphocytes with marek's disease virus. | suspension cultures of splenic lymphocytes, incubated at 41 degrees c, became infected with marek's disease virus (mdv) following exposure to a) other infected lymphocytes, b) infected chicken kidney monolayer cultures, or c) cell-free mdv. both viral antigen expression and virus isolation could be demonstrated after more than 40 passages made by the addition of fresh spleen cells at 2- to 3-day intervals. susceptibility of spleen cells from bursectomized chickens was markedly lower than that of ... | 1982 | 6287084 |
| molecular-biological characterization of marek's disease virus. i. identification of virus-specific polypeptides in infected cells. | 1982 | 6287716 | |
| molecular-biological characterization of marek's disease virus. ii. differentiation of various mdv and hvt strains. | 1982 | 6287717 | |
| circular forms of viral dna in marek's disease virus-transformed lymphoblastoid cells. | the state of viral dna in three marek's disease virus (mdv)-transformed chicken cell lines (mdcc-msb-1, mdcc-hp-2, mdcc-rp-1) was investigated by cscl-density gradient centrifugation, and sedimentation analysis in neutral glycerol gradients. each cell line contained mdv dna integrated into the host cell genome. additionally, free viral dna could be identified in mdcc-msb-1 and mdcc-hp-2 cells, sedimenting at about 100 s, and banding at the position of circular dna in cscl-ethidiumbromide gradien ... | 1982 | 6287971 |
| in vivo study of interference between herpes and influenza viruses. | 1982 | 6288823 | |
| [extraction and freeze-drying of herpes virus c3-1 isolated from turkeys]. | possibilities for producing dried preparations of turkey herpes virus c3-1 by use of various ultra-sound desintegrator-systems and different ways of processing the cell suspensions containing the virus were studied. it was established that cell-less virus of a 1.5 to 2 log smaller quantity than the initial virus containing cell suspension can be produced by ultrasound generator with an indirect action -- a vibrating membrane, which transmits the impulses through a liquid medium and the container ... | 1982 | 6291223 |
| impaired host resistance against local jmv marek's disease tumors after thymectomy and after bursectomy. | 1982 | 6293284 | |
| inhibitory activity of cytosine arabinoside on marek's disease virus. | the presence of ara-c in growth medium at concentrations of 10(-4) to 10(-7) m completely or partially suppressed the formation of plaques specific for mdv or hvt and decreased proportionally the growth of hprs line 1 lymphoblastoid cells. administration of ara-c to chickens immediately after infection with mdv (1 mg/chicken/day i.p. for 5 days) reduced the incidence of marek's disease by 50%. thus ara-c appears to be an inhibitor of marek's disease. | 1982 | 6293895 |
| partial restriction map of marek's disease virus dna. | a partial restriction map of marek's disease virus (mdv) dna was constructed by digestion with endonucleases bamhi, bg/i and smai and by blotting hybridization. the data suggest that there is a terminal heterogeneous sequence at least on one end of the mdv dna molecule. the data did not reveal four different orientations of the terminal fragments of mdv dna molecules despite the observation that mdv dna contains inverted repeat sequences as also present in herpes simplex virus (hsv) dna molecule ... | 1982 | 6293927 |
| a monoclonal antibody reactive with marek's disease tumor-associated surface antigen. | an antibody-secreting hybridoma, rph-6, to marek's disease tumor-associated surface antigen (matsa) was produced by somatic-cell hybridization between the mouse myeloma sp2/o-ag/14 and spleen cells from msb1 immunized mice. the antibody reacted with 95 to 100% of the cells from eight of 10 chicken md cell lines and one of two turkey md cell lines. it did not react with chicken md cell lines rp1 and sk3, turkey md cell line rp19, lymphoid leukosis (ll) cell lines, rp9 and rp12, and reticuloendoth ... | 1983 | 6294177 |
| [virus and cancer]. | 1981 | 6294911 | |
| [relationship between the genetic polymorphism of alkaline phosphatase and the resistance of broiler poultry to marek's disease virus]. | studied was the polymorphism of alkaline phosphatase in blood plasma samples taken from 507 poulets and cocks of lines 66 and 77 of the cornish breed, and lines 88 and 99 of the white plymouth rock breed. the birds were divided into three groups to test their resistance to marek's disease: (1) controls, (2) infected with the virus of marek's disease with 3 subgroups--resistant birds, slightly susceptible ones, and strongly susceptible ones, and (3) contacts, with two subgroups--survivals and bir ... | 1982 | 6294972 |
| [breed, line and sex differences of phagocytic activity in relation to broiler poultry resistance to marek's disease virus]. | the phagocytic activity was studied of 421 poulets and cocks of lines 66 and 77 of the cornish breed, and lines 88 and 99 of the white plymouth rock breed. breed and linear differences were noticed in the changes of the phagocytic activity (phagocytic number and phagocytic index) with birds infected with the virus of marek's disease. the rise of the phagocytic activity with lines 66 and 77 was slighter as compared with that of the white plymouth rock lines 88 and 99. strongest were the changes i ... | 1982 | 6294973 |
| characterization of non-oncogenic marek's disease virus-infected and turkey herpesvirus-infected lymphocytes. | 1982 | 6296281 | |
| propagation of virulent and avirulent turkey hemorrhagic enteritis virus in cell culture. | virulent and apathogenic isolates of turkey hemorrhagic enteritis virus (hev) were successfully propagated in lymphoblastoid cell lines of turkey origin, whereas spleen and kidney cell cultures from hev-infected turkeys failed to replicate the virus. the lymphoblastoid cell lines used were mdtc-rp16 and mdtc-rp19, which were previously established from tumors induced by marek's disease virus in turkeys. virus replication followed co-cultivation of lymphoblastoid cells with spleen cells from hev- ... | 1982 | 6297447 |
| influence of maternal antibody on efficacy of embryo vaccination with cell-associated and cell-free marek's disease vaccine. | vaccination with turkey herpesvirus (hvt) of 18-day-old chicken embryos from a commercial source or from a cross (15 x 7) of two inbred lines induced better protection against early post-hatch challenge with virulent marek's disease virus (mdv) than vaccination at hatch, despite the presence in embryos of maternally derived antibodies to hvt or to hvt and mdv. however, 50%-protective-dose (pd50) assays revealed that maternal antibodies in embryos reduced vaccine efficacy. the pd50 assays were co ... | 1982 | 6297450 |
| marek's disease virus (mdv) antigens in the feather follicle epithelium: difference between oncogenic and nononcogenic mdv. | feather tips from chickens infected with oncogenic or nononcogenic marek's disease virus (mdv) were individually tested for mdv antigens by the agar-gel-precipitin test to compare antigen contents of feather follicles infected with oncogenic or nononcogenic mdv. there was a significant difference between oncogenic and nononcogenic mdv in the development of mdv antigens in the feather follicle epithelium of infected chickens. significantly more feather tips from chickens infected with oncogenic m ... | 1982 | 6297453 |
| marek's disease--survey on vaccination failures. | twelve years after the initial development of efficacious vaccines against marek's disease--prepared mostly from hvt--and their subsequent use on a large scale, there is a general opinions that failures have been increasing in incidence and in severity. many factors have been held responsible for these failures, and, though efforts are being made to obviate them, no acceptable solutions have been found sofar in the control of the disease in the field. if mistakes in the preparation, storage hand ... | 1982 | 6299837 |
| the epidemiology of avian herpesviruses in veterinary medicine. | there are ten avian herpesviruses, which have been isolated from eight orders. six of these are of veterinary importance: pacheco's parrot disease virus, pigeon herpesvirus, duck plague virus, infectious laryngotracheitis virus, herpesvirus of turkeys and marek's disease virus. the knowledge on the epidemiology of each virus and the disease it causes is discussed. features in common to infections with most avian herpesviruses are: infection is persistent in individuals and ubiquitous in populati ... | 1982 | 6299838 |
| marek's disease vaccines. | marek's disease (md) vaccines have been in almost universal use since the early 1970's and constitute the first effective practical means for the control of any neoplastic disease in man or animals. five types have been described: 1) attenuated variants of oncogenic strains (serotype 1) of marek's disease virus (mdv) produced by serial passage in cultured cells; 2) naturally nononcogenic mdv strains (serotype 2); 3) nononcogenic herpesvirus from turkeys (hvt, serotype 3); 4) inactivated cells or ... | 1982 | 6299850 |
| [prevention of marek's disease using 3 vaccines in chickens belonging to different genetic varieties of meat producers]. | 1982 | 6299854 | |
| suppression of humoral immunity in chickens prevents transient paralysis caused by a herpesvirus. | marek's disease virus (mdv)3 is a highly oncogenic herpesvirus that usually causes visceral lymphomas and lymphoid infiltration of the peripheral nerves in chickens. a relatively rare encephalitic condition, first found in farm flocks and referred to as transient paralysis (tp), is also caused by mdv(1). tp symptoms occur 9 to 11 days after mdv inoculation and range from mild ataxia to profound coma. most birds recover by 24 to 72 hr after onset of symptoms, although severely affected birds may ... | 1983 | 6300237 |
| marek's disease herpesviruses. iv. molecular characterization of marek's disease herpesvirus a antigen. | marek's disease herpesvirus a antigen (mdhv-a) was identified as a 61,000- to 65,000-dalton glycoprotein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation from the culture medium of both [35s]methionine- and [14c]glucosamine-labeled infected cells by specific rabbit serum directed against mdhv-a. rigorous identification was accomplished by selective blocking of this specific immunoprecipitation of the glycoprotein with purified mdhv-a that was isolated at its ... | 1983 | 6300460 |
| characterization of marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells. | leukocyte suspensions derived from genetically marek's disease (md)-resistant n-line and md-susceptible p-line chickens were fractionated at various times after exposure to the jm-10 clone of md virus. at 3 and 5 days post exposure (dpe), during the productive-restrictive (cytolytic) phase, most infected spleen and thymus leukocytes were found to be low-density, nylon wool-adherent cells that possessed fc receptors and surface ia and igm and were depleted by carbonyl iron treatment. this was tru ... | 1983 | 6300499 |
| failure of herpesvirus saimiri to enhance atherogenesis in owl monkeys (aotus trivirgatus). | a retrospective study was performed to evaluate the potential of a mammalian oncogenic virus, herpesvirus saimiri, to cause atherosclerosis in owl monkeys (aotus trivirgatus). this was undertaken since an avian oncogenic herpesvirus, marek's disease virus, does so in chickens. data from earlier studies were reviewed and 3 groups of animals were selected. these included 23 animals infected with herpesvirus that died an average of 156 days later with malignant lymphoma; 11 infected an average of 2 ... | 1983 | 6301514 |
| embryo vaccination against marek's disease with serotypes 1, 2 and 3 vaccines administered singly or in combination. | marek's disease virus (mdv) vaccines of serotypes 1 and 2 administered in 18-day-old embryonated eggs induced better protection against post-hatch challenge at 3 days with virulent mdv than vaccines given at hatch. embryonal vaccination with a polyvalent vaccine containing equal quantities of serotypes 1 and 2 of mdv and serotype 3 virus (turkey herpesvirus, hvt) was also significantly more effective than post-hatch vaccination. these and earlier results indicate that protective efficacy of sing ... | 1983 | 6307248 |
| studies on the role of macrophages in marek's disease of the chicken. | macrophage activity in relation to marek's disease was investigated by determining phagocytic indices in vivo and by examining virus plaque-inhibiting activity of peritoneal macrophages in vitro. no correlation was observed between phagocytic index and resistance in different genetic strains of chickens. infection with marek's disease virus increased both phagocytic indices and the plaque-inhibiting activity of peritoneal macrophages, more so in susceptible than in resistant chickens. there was ... | 1983 | 6312038 |
| simultaneous vaccination of one-day-old broiler chickens with tenosynovitis virus and turkey herpesvirus. | an attenuated tenosynovitis virus vaccine and turkey herpesvirus (hvt) vaccine mixed prior to vaccination were injected subcutaneously into one-day-old chicks. in laboratory and field trials there did not appear to be any interference, because chickens vaccinated with both vaccines were effectively immunized against challenge with virulent marek's disease virus or tenosynovitis virus when compared with unvaccinated chickens or chickens vaccinated with either hvt or tenosynovitis virus. the combi ... | 1983 | 6312445 |
| viruses in the etiology of atherosclerosis. | to examine the possible role of viruses in the etiology of atherosclerosis, we searched for the presence of viral genomes in arterial tissues by in situ hybridization. because chickens infected with marek disease virus, a herpesvirus, develop atherosclerotic lesions after infection, we looked for the presence of herpesvirus or parts thereof in human artery wall tissue, particularly in individuals with evidence of atherosclerosis. herpesvirus probes were used on specimens of aortic wall removed f ... | 1983 | 6312457 |
| establishment of lymphoblastoid cell lines from marek's disease primary tumors. | seven lymphoblastoid cell lines were developed from 15 marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). these cell lines are designated as mdcc-rp22 through mdcc-rp29. tumor materials were from 151(5) x 7(1) chickens infected with either jm-111s clone or the ga-22 clone of marek's disease virus. factors contributing to the establishment of cell lines include, in addition to the r ... | 1983 | 6314315 |
| kinetics of phytohemagglutinin response in chickens infected with various strains of marek's disease virus. | phytohemagglutinin (pha) response of whole blood lymphocytes from white leghorn inbred line 7(2) chickens infected with various strains of marek's disease virus (mdv) was monitored sequentially for 6 weeks postinfection. a significant difference between jm and ga strains was shown. a two-phase depression in the pha response was observed in chickens infected with the jm strain. early depression occurred 1 week postinfection and was followed by recovery a week later. the second depression occurred ... | 1983 | 6314973 |
| field trials with a bivalent vaccine (hvt and sb-1) against marek's disease. | white leghorn chickens on five farms were given a bivalent marek's disease (md) vaccine consisting of turkey herpesvirus (hvt) and sb-1 (a nononcogenic md virus); other chickens received only hvt. the farms had histories of "vaccination failures," presumably owing to an exceptionally virulent challenge md virus. the bivalent vaccine uniformly protected chickens better than hvt alone between 12 and 16-20 weeks of age, when serious md losses occurred. during that period, total mortality in groups ... | 1983 | 6314982 |
| prevalence of antibodies to infectious bursal disease virus serotypes i and ii in 75 ohio chicken flocks. | serum samples from 75 ohio layer flocks were tested for antibodies to infectious bursal disease virus (ibdv) serotypes i and ii using a virus-neutralization test. infectious bursal disease had been diagnosed in three of the 75 flocks. antibody titers to serotype i were detected in 77% of the flocks, and antibody titers to serotype ii were detected in 47% of the flocks. of the eight flocks known to have been vaccinated using a serotype i ibdv vaccine, all had antibody titers to serotype i, and fo ... | 1983 | 6314983 |
| marek's disease virus-induced transient paralysis: a comparison of lesions in susceptible and resistant lines of chickens. | a study was conducted to determine the causative lesion of marek's disease virus-induced transient paralysis. brain histologic lesions were quantitated and compared in genetically resistant and susceptible inbred chickens. comparisons were made 11 days post inoculation (pi) of virus when clinical signs were evident in susceptible birds and on day 17 pi when these signs had remitted. clinically-affected birds from the susceptible line on day 11 pi had consistent evidence of neuropil vacuolation c ... | 1983 | 6316708 |
| avian lymphoproliferative diseases and their virus associations. | 1983 | 6316767 | |
| in vivo characteristics of a transplantable marek's disease lymphoblastoid cell line, mdcc-msb1-41c. | a marek's disease (md) lymphoblastoid cell line, mdcc-msb1-41c, was highly transplantable and lethal for chickens. autopsies showed extensive metastasis in various organs. the transplantabilities of the parent cell line, mdcc-msb1, and another derivative line, mdcc-msb1-33c, were transient. md virus (mdv) could be isolated from the kidneys but not from the peripheral blood leukocytes of chickens inoculated with the msb1-41c cell line. in addition, anti-mdv antibodies were produced both in chicke ... | 1983 | 6316900 |
| [morphological studies of lymphatic tissue of chick livers following infection with marek's disease virus and turkey herpes virus]. | 1983 | 6318683 | |
| restriction endonuclease analysis of marek's disease virus dna and homology between strains. | the restriction endonuclease patterns of viral dna obtained from serotypes 1 and 2 strains of marek's disease virus have been compared and homology between the strains examined by hybridization. the results have shown that hprs 16 (serotype 1) dna has a structure similar to its attenuated variant hprs 16/att except for a few fragments that are present only in the virulent strain. evidence was obtained which suggested that these restriction fragments contained repeat sequences and that insertion ... | 1983 | 6319562 |
| genetic relatedness of virulent and avirulent strains of marek's disease virus. | comparative studies using southern blot hybridization indicated that the avirulent strains of marek's disease virus (mdv) were probably generated through dna rearrangement that occurred in the limited area(s) of the virulent mdv genome. | 1984 | 6320782 |
| further characterization of marek's disease virus-infected lymphocytes. i. in vivo infection. | previous reports from this laboratory identified bursa-derived lymphocytes (b cells) and non-b cells as the predominant cell types respectively involved in the early cytolytic and subsequent latent infection of chickens with marek's disease virus (mdv). it was not known whether these differences were qualitative or quantitative or if the method for detection of latent infection (viral antigen production after 48 h of in vitro cultivation) was sensitive enough. to further define the cells involve ... | 1984 | 6321364 |
| further characterization of marek's disease virus-infected lymphocytes. ii. in vitro infection. | lymphocyte cultures from chicken spleens had been shown to be susceptible to in vitro infection by marek's disease virus (mdv)4 in an earlier report from this laboratory. in that study, virus infection was evidenced by virus isolation and detection of viral internal antigen (via) 2 days post inoculation (dpi), and serial passage was accomplished by adding fresh spleen cells at 2-day intervals. the susceptible cells were identified as bursa-derived lymphocytes (b cells). using a dual fluorescence ... | 1984 | 6321365 |
| the use of reference preparations in virus content testing of vaccines. | a series of virus content tests on newcastle disease, avian infectious bronchitis and marek's disease vaccines was carried out over a period of several years on commercial products together with a standard preparation of each virus. analysis of the results of marek's disease vaccine titrations revealed a good correlation between plaque count and the ratio obtained for the standard vaccine tested in parallel. expression in relation to a reference preparation is therefore of value to vaccine manuf ... | 1984 | 6321508 |
| identification with monoclonal antibodies of glycoproteins of marek's disease virus and herpesvirus of turkeys related to virus neutralization. | by use of monoclonal antibodies cross-reactive with marek's disease virus and herpesvirus of turkeys, three glycoproteins (for marek's disease virus, gp115/110, gp63, and gp50; for herpesvirus of turkeys, gp115, gp62 and gp52) related to virus neutralization were identified. immunization of chickens or rabbits with these glycoproteins purified by affinity chromatography resulted in production of neutralizing antibodies. | 1984 | 6321752 |
| [vaccinating properties of the kekava 55 strain of attenuated marek's disease virus]. | two attenuated strains of oncogenic herpes virus, marek's disease virus, were obtained: one by cultivation in chick fibroblast cultures at 37 degrees c, the other at 41 degrees c, i. e. at chicken body temperature. both attenuated strains throughout a large number of passages retain high vaccination efficacy, on the average 76% and 77.3%, respectively. the virus attenuated at 41 degrees c is superior to that attenuated at 37 degrees c in higher reproduction rate in vitro and, possibly, in a grea ... | 1983 | 6322454 |
| [early mortality syndrome in chickens following double infection with marek's disease virus and chicken anemia agent]. | 1983 | 6322482 | |
| in vivo and in vitro infections of chicken lymphocytes with marek's disease virus or herpesvirus of turkeys. | 1983 | 6323800 | |
| [protecting chickens against marek's disease under laboratory conditions by the administration of marvak, a czechoslovak produced vaccine and vaccine imported from east germany]. | laboratory trials were conducted to compare the efficiency of two vaccines against marek's disease (md) on the basis of the dynamics of post-vaccination viraemia and by means of a challenge test. two groups of the hx-sl hybrid layers, each containing 40 birds, were vaccinated with the marvak cellular vaccine manufactured in czechoslovakia (100 pfu per chick) or with the insel riems vaccine manufactured in the gdr (1000 pfu per chick). in weekly intervals, half the birds of each group were tested ... | 1984 | 6326372 |
| aus in the prevention of marek's disease. | aminoureidosulfone (aus) was used as a feed additive to prevent mortality in marek's disease (md)-infected chickens in the laboratory. chicks infected with md virus (5000 plaque-forming units) at 1 day of age were given aus in the feed from 1 day of age until the experiment was terminated. aus, at all concentrations tested, reduced mortality due to marek's disease. chickens receiving aus at the 0.002% level had a 4% mortality rate, those receiving 0.005% had 9% mortality, and those receiving 0.0 ... | 1984 | 6326736 |
| immunization against marek's disease transplantable cell lines. | continuous passage of mdcc-rp1, a highly tumorigenic marek's disease (md) lymphoblastoid cell line, in cell culture resulted in a gradual loss in ability of the cell line to cause progressive tumors in susceptible day-old chicks. inoculation of day-old chicks with high-cell-culture-passaged (187th to 417th) nontumorigenic mdcc-rp1 cells gave excellent protection against challenge at 8 days with low-passaged tumorigenic mdcc-rp1 cells but failed to protect against primary tumors caused by inocula ... | 1984 | 6326737 |
| field trials to test the efficacy of polyvalent marek's disease vaccines in broilers. | the efficacies of trivalent (md11/75c + sb-1 + hvt), bivalent (sb-1 + hvt), and turkey herpesvirus (hvt) vaccines against marek's disease (md) were compared in commercial broiler flocks in four trials involving 11 farm locations and 486,300 chickens. in all four trials, chickens receiving polyvalent vaccines had lower leukosis (md) condemnation rates than chickens vaccinated with hvt alone; when data were summarized for each vaccine type in each trial, condemnation rates for the bivalent- or tri ... | 1984 | 6326743 |
| structure of marek's disease virus dna: detailed restriction enzyme map. | purified virion dna (120 x 10(6) molecular weight [mw]) of marek's disease virus strain ga was cleaved with bamhi restriction endonuclease, and 27 out of the 29 fragments were cloned into bacterial plasmids. restriction maps for bamhi, bgli, and smai endonucleases were constructed. the genomic structure of marek's disease virus dna was found to be similar to that of herpes simplex virus types 1 and 2. a long unique region (75 x 10(6) mw, located at 10 x 10(6) to 85 x 10(6) mw [10-85] from the le ... | 1984 | 6328029 |