Publications

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cardiopulmonary helminth parasites of red foxes (vulpes vulpes) in catalonia, northeastern spain. 200515683774
karyotype of canine soft tissue sarcomas: a multi-colour, multi-species approach to canine chromosome painting.many canine tumour types represent useful models for tumours also found in humans. studies of chromosomal abnormalities in canine tumours have been impeded by the complexity of the canine karyotype (2n = 78), which has made accurate identification of rearranged chromosomes difficult and laborious. to overcome this difficulty we have developed a seven-colour paint system for canine chromosomes, with six sets of chromosome paints covering all chromosomes except y. several pairs of canine autosomes ...200415702421
detecting danger--or just another odorant? olfactory sensitivity for the fox odor component 2,4,5-trimethylthiazoline in four species of mammals.2,4,5-trimethylthiazoline (tmt) is a volatile component of the anal gland secretion of the red fox and elicits behavioral and physiological fear responses in the rat. using instrumental conditioning paradigms, we determined olfactory detection thresholds for tmt in three rats, a natural prey species of the red fox, and compared their performance to that of three squirrel monkeys, three spider monkeys and four pigtail macaques, all non-prey species of the red fox. we found that the rats were able ...200415708773
field evaluation of an intravital diagnostic test of echinococcus multilocularis infection in red foxes.echinococcus multilocularis parasitizes the small intestine of red foxes (vulpes vulpes) and other carnivores, and has a wide distribution throughout the northern hemisphere. this cestode is the causative agent of human alveolar echinococcosis, a life-threatening helminth zoonosis. in 2000-2002, 2130 red foxes were examined for its presence in slovakia, with a total prevalence of 30.7%. the data on occurrence were obtained by the combination of necropsy of small intestines from red foxes and cop ...200415725534
first record of trichinella pseudospiralis in the slovak republic found in domestic focus.infection of trichinella spp. is widespread among wildlife in slovakia and the red fox (vulpes vulpes) is the main reservoir of trichinella britovi. trichinella spiralis has been rarely documented in sylvatic and domestic animals of this country. during routine examination of domestic pigs at the slaughter, trichinella larvae were detected by artificial digestion in a domestic pig of a large-scale breeding farm in eastern slovakia. the parasite has been identified by molecular (pcr) and biochemi ...200415725537
oral vaccination of wildlife against rabies: opportunities and challenges in prevention and control.rabies is an acute, progressive, fatal encephalitis caused by viruses in the family rhabdoviridae, genus lyssavirus. rabies virus is the representative member of the group. warm-blooded vertebrates are susceptible to experimental infection, but major primary hosts for disease perpetuation encompass bats and mammalian carnivores. the dog is the global reservoir, and important wild carnivores include foxes, raccoons, skunks, and mongoose, among others. traditionally, reliance upon long-term, wides ...200415742629
elimination of terrestrial rabies in western european countries.since the late 1930s, the red fox (vulpes vulpes) has been the main vector of rabies in europe. practically, decimation of fox population did not prevent the spread of the disease. the only efficient method to control wildlife rabies consisted in using oral vaccination by depositing vaccine baits containing a capsule or a plastic sachet filled with an attenuated anti-rabies liquid vaccine throughout fox habitats. several live virus vaccines have been and are currently being used: the sad b19 and ...200415747421
epizootiologic investigations of parvovirus infections in free-ranging carnivores from germany.to assess if wild carnivores in germany play a role in the epizootiology of canine parvovirus (cpv) infection, seroprevalences against cpv in free-ranging carnivores (n=1,496) from selected urban and rural areas were compared. antibodies against cpv were found in sera from red foxes (vulpes vulpes; 136 of 1,442; 9%), raccoon dogs (nyctereutes procyonides; two of 33; 6%), stone martens (martes foina; four of 13; 31%), and pine martens (martes martes; one of two) using the hemagglutination-inhibit ...200515827228
endemic alveolar echinococcosis in southern belgium?until now, belgium has been considered as a low-risk country for alveolar echinococcosis. however it was recently demonstrated by necropsy series that, in some parts of southern belgium (wallonia), up to 51% of the red foxes (vulpes vulpes) may be infected by e. multilocaris. the authors, working in a university hospital in southern belgium, described in 2002 the first autochthonous belgian case of hepatic alveolar echinococcosis. more importantly, in 2004, they diagnosed three other patients wi ...200515832579
epidemiological aspects of canine visceral leishmaniosis in the islamic republic of iran.an epidemiological study to examine the sero-prevalence of zoonotic visceral leishmaniosis (zvl) among domestic and wild canines in endemic foci of iran was carried out during 1999-2003 to assess the distribution of the disease and the possible association between infection in dogs, wild canines and people. anti-leishmanial antibodies were detected by the direct agglutination test (dat). parasitological study was performed for all captured wild canines and were detected in some of the seropositi ...200515845279
the proto-oncogene c-kit maps to canid b-chromosomes.plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or b-chromosomes. it is generally believed that b-chromosomes lack major genes and represent parasitic and selfish elements of a genome. here we report, for the first time, the localization of a gene to b-chromosomes of mammals: red fox (vulpes vulpes) and two subspecies of raccoon dog (nyctereutes procyonoides). identification of the proto-oncogene c-kit on b-chromosomes of two canidae species th ...200515861301
salmonella isolates from wild birds and mammals in the basque country (spain).the authors investigated the prevalence of salmonella spp. in 205 wild birds and mammals belonging to 45 species during the years 2001 and 2002 in the basque country (spain). salmonella was isolated from 16 (7.8%) animals. the prevalence was 8.5% (7/82) in birds, and 7.2% (9/123) in mammals. nine serotypes, all of them belonging to the species salmonella enterica, were identified: two isolates of typhimurium (from 1/3 griffon vultures [gyps fulvus], and 1/5 sparrowhawks [accipiter nisus]); one o ...200415861885
cloning and sequencing of cdna encoding for the testis-specific fox (vulpes vulpes) sperm polypeptide vb of the cytochrome c oxidase.identification of fox (vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. we report here the cloning and sequencing of fsp8, a fox sperm protein of 14.7 kd. fsp8 was isoantigenic in foxes, as it was recognized by sera of both male and female foxes immunized with fox sperm proteins. no glycosylation was detected, on fsp8, as shown both by deglycosylation assay and lectin labeling. to determine the fsp8 sequence, the nh2-termin ...200515866998
seminal concentration of trace elements in fox and relationships to spermatozoa quality.concentrations of copper, zinc, iron, cadmium, lead, and nickel in the semen of foxes (vulpes vulpes, n = 10), microscopic analysis of occurrence of pathological spermatozoa, and correlations of these elements with pathological forms were studied. samples were analyzed by using an atomic absorption spectrophotometer. for analysis of pathological spermatozoa semen samples fixed with hancock's solution and stained with giemsa were prepared. for each fox at least 1000 spermatozoa were evaluated. th ...200515887577
stepwise mutation likelihood computation by sequential importance sampling in subdivided population models.an importance sampling algorithm for computing the likelihood of a sample of genes at loci under a stepwise mutation model in a subdivided population is developed. this allows maximum likelihood estimation of migration rates between subpopulations. the time to the most recent common ancestor of the sample can also be computed. the technique is illustrated by an analysis of a data set of australian red fox populations.200515890376
status of oral rabies vaccination in wild carnivores in the united states.persistence of multiple variants of rabies virus in wild chiroptera and carnivora presents a continuing challenge to medical, veterinary and wildlife management professionals. oral rabies vaccination (orv) targeting specific carnivora species has emerged as an integral adjunct to conventional rabies control strategies to protect humans and domestic animals. orv has been applied with progress toward eliminating rabies in red foxes (vulpes vulpes) in western europe and southern ontario, canada. mo ...200515896404
[surveillance on severe acute respiratory syndrome associated coronavirus in animals at a live animal market of guangzhou in 2004].to study the prevalence of severe acute respiratory syndrome coronavirus (sars-cov) like virus in animals at a live animal market of guanzhou in 2004 before and after culling of wild animal action taken by the local authority, in order to predict the re-emerging of sars from animal originals in this region.200515921605
intestinal establishment and reproduction of adult trichinella spp. in single and mixed species infections in foxes (vulpes vulpes).intestinal establishment and reproduction of adult trichinella spiralis, trichinella nativa, trichinella britovi and trichinella pseudospiralis were examined as single species or mixed species infections in foxes. this is the first study of intestinal dynamics of trichinella spp. in a carnivore model and the results suggest that the intestinal phase is relatively short as only very few worms were recovered 10 days post-inoculation (dpi). in mixed species infection with equal doses of t. nativa a ...200515925724
studies on vertical transmission of trichinella spp. in experimentally infected ferrets (mustela putorius furo), foxes (vulpes vulpes), pigs, guinea pigs and mice.vertical transmission of trichinella spiralis was evaluated in ferrets (n=21), foxes (n=11), pigs (n=12), guinea pigs (n=16), and mice (n=41). the placental barrier to be crossed by migratory trichinella larvae varies structurally in different animal species. ferrets and foxes have an endotheliochorial placenta structure, guinea pigs and mice a haemochorial, and pigs an epitheliochorial placenta. the non-encapsulating trichinella pseudospiralis larvae have an extended muscle migration prior to e ...200515925725
toxocara canis in experimentally infected silver and arctic foxes.in two experiments, thirty-six farm foxes of two species were inoculated with various doses of infective toxocara canis eggs or tissue larvae isolated from mice. in experiment i, six adult arctic foxes (alopex lagopus; 11-month old) were each inoculated with 20,000 eggs and sacrificed 100, 220, or 300 days post infection (dpi), while ten silver fox cubs (vulpes vulpes; 6-9-week old) were infected with varying doses of eggs (30-3000) and necropsied 120 dpi. in experiment ii, two groups of five cu ...200515986242
prevalence of serum antibodies to canine adenovirus and canine herpesvirus in the european red fox (vulpes vulpes) in australia.to determine the seroprevalence and aspects of the epidemiology of canine adenovirus (cadv) and canine herpesvirus (cahv-1) in european red foxes (vulpes vulpes) in australia.200515986915
echinococcus multilocularis in red foxes (vulpes vulpes) of the italian alpine region: is there a focus of autochthonous transmission?alveolar echinococcosis, caused by the metacestode of echinococcus multilocularis, is a zoonosis with a wider distribution area than described in the past. fox populations living in the alpine regions of italy had been considered free from this parasite until 2002, when two infected foxes (vulpes vulpes) were detected in the bolzano province (trentino alto adige region) near the austrian border. the aim of this work was to evaluate the prevalence of infection in red fox populations from five ita ...200515998516
comparative aspects of sperm membrane fatty acid composition in silver (vulpes vulpes) and blue (alopex lagopus) foxes, and their relationship to cell cryopreservation.cryogenic protocols have been developed for the storage of farmed silver fox (vulpes vulpes) spermatozoa. however, these same protocols and modifications of these protocols have failed to satisfactorily preserve spermatozoa collected from farmed blue foxes (alopex lagopus). because cryogenic success has been linked to membrane composition, the plasma membrane lipid composition of farmed blue fox and silver fox spermatozoa was studied. silver fox spermatozoal membranes have significantly higher l ...200516040024
copro-diagnosis of echinococcus multilocularis by a nested pcr in red foxes (vulpes vulpes) from northern italy. 200416044705
complex interactions among mammalian carnivores in australia, and their implications for wildlife management.mammalian carnivore populations are often intensively managed, either because the carnivore in question is endangered, or because it is viewed as a pest and is subjected to control measures, or both. most management programmes treat carnivore species in isolation. however, there is a large and emerging body of evidence to demonstrate that populations of different carnivores interact with each other in a variety of complex ways. thus, the removal or introduction of predators to or from a system c ...200516094805
tmt-induced autonomic and behavioral changes and the neural basis of its processing.one of the main interests in the field of neuroscience is the investigation of the neural basis of fear. during recent years, an increasing number of studies have used trimethylthiazoline (tmt), a component of red fox feces, as a stimulus to induce fear in predator naive rats, mice, and voles. the aim of the present review is to summarize these studies. we present an overview to the autonomic and behavioral changes that are induced by tmt exposure. then, we summarize the small number of studies ...200516099043
diseases and mortality in free-ranging brown bear (ursus arctos), gray wolf (canis lupus), and wolverine (gulo gulo) in sweden.ninety-eight brown bears (ursus arctos), 20 gray wolves (canis lupus), and 27 wolverines (gulo gulo), all free-ranging, were submitted to the national veterinary institute, uppsala, sweden, during 1987-2001 for investigation of diseases and causes of mortality. the most common cause of natural death in brown bears was infanticide. infanticide also was observed in wolverines but not in wolves. traumatic injuries, originating from road or railway accidents, were the most common cause of death in w ...200516107663
bovine tuberculosis in a free ranging red fox (vulpes vulpes) from doñana national park (spain).during 1997 and 1998, a survey of iberian carnivores was conducted to study the epidemiology of bovine tuberculosis in the doñana national park and surrounding areas in southwestern spain. post-mortem examinations were done on seven red foxes (vulpes vulpes), two egyptian mongoose (herpestes ichneumon), one weasel (mustela nivalis), two genets (genetta genetta), one iberian lynx (lynx pardinus), one eurasian badger (meles meles), and two polecats (mustela putorius). lesions suggestive of bovine ...200516107680
seasonal 4-year investigation into the role of the alpine marmot (marmota marmota) as a carrier of zoophilic dermatophytes.two hundred and six samples of alpine marmot (mamota marmota) hair (148 from adults and 58 from young subjects), 102 soil samples from the entrances to the burrows of the above individuals and 20 control specimens (obtained from adjoining areas away from the burrow systems where the rodents are not usually present) were examined from may 1994 to september 1997. seventy-five isolates belonging to six species of dermatophytes were found in 69 of the 206 hair samples examined (33.5%). two species w ...200516110784
a canine x chromosome painting probe applied to four canid species: close relationship of a heterochromatic-like sequence between the dog and the blue fox.microdissection of chromosomes is an invaluable tool to physically isolate single chromosomes, chromosome-arms or chromosome-bands and, subsequently generate painting probes with which numerical or structural aberrations of chromosomes can be studied. in addition, such painting probes can be used to compare karyotypic relationships among mammalian species. for the present study a canine whole x chromosome painting probe was prepared by means of conventional microdissection and degenerate-oligonu ...200516130457
red fox (vulpes vulpes) cannibalistic behaviour and the prevalence of trichinella britovi in nw italian alps.food habits of the red fox (vulpes vulpes) were studied in the aosta valley region (nw italian alps) and were related to the prevalence of trichinella infection in the red fox itself and in two mustelid species (the stone marten (martes foina) and the badger (meles meles)). the search of trichinella by the automatic digestion of muscles samples led us to determine a prevalence of 3.5+/-1.2% in red foxes, 7.9+/-4.3% in stone martens and 1.9+/-1.8% in badgers, with no significant differences among ...200516151732
a molecular phylogeny of the canidae based on six nuclear loci.we have reconstructed the phylogenetic relationships of 23 species in the dog family, canidae, using dna sequence data from six nuclear loci. individual gene trees were generated with maximum parsimony (mp) and maximum likelihood (ml) analysis. in general, these individual gene trees were not well resolved, but several identical groupings were supported by more than one locus. phylogenetic analysis with a data set combining the six nuclear loci using mp, ml, and bayesian approaches produced a mo ...200516213754
distribution of crenosoma vulpis and eucoleus aerophilus in the lung of free-ranging red foxes (vulpes vulpes).crenosoma vulpis and eucoleus aerophilus are nematode parasites that can cause verminous pneumonia in wild carnivores. there is a paucity of information regarding the distribution of parasites in the lungs and the relationship between histopathological and parasitological diagnoses in naturally infected foxes. the objectives of this study were: first, to study the lobar and airway distribution of c. vulpis and e. aerophilus in wild red foxes and second, to investigate the relationship between fe ...200516312245
cloning of fox (vulpes vulpes) il2, il6, il10 and ifngamma and analysis of their expression by quantitative rt-pcr in fox pbmc after in vitro stimulation by concanavalin a.the immune response in the fox (vulpes vulpes), despite the success of the oral rabies vaccine is not well characterised, and specific immunological tools are needed. a quantitative rt-pcr using sybr green to investigate fox cytokine expression after antigen pbmc in vitro re-stimulation is presented here. first, we cloned by homology with dog cytokine sequences the fox il2, il6, il10, ifngamma and a partial 18s sequence. fox specific primers were then defined and used to set up a species-specifi ...200616321447
prevalence of trichinella larvae and extra-intestinal nematodes in norwegian red foxes (vulpes vulpes).a survey of the parasitic fauna of the norwegian red fox (vulpes vulpes) population was carried out in 1994/1995 and 2002-2005. all foxes were killed during the licensed hunting season from october to april and, in total, 393 animals from all regions of the country were examined. the present study details the results of extra-intestinal nematode and trichinella larvae examinations. all individuals were examined for trichinella, using routine digestion methods. parasitological examination of the ...200616378689
echinococcus multlocularis infections of rural, residential and urban foxes (vulpes vulpes) in the canton of geneva, switzerland.we examined 267 red foxes (vulpes vulpes) from the canton of geneva, switzerland, for intestinal infections with echinococcus multilocularis. this region is situated in the core area of the endemic range of this zoonotic cestode in central europe. several factors were taken into account and urbanisation level appeared to be the most explicative to describe observed differences. the prevalence decreased significantly from rural and residential areas (prevalence of 52%, ci 43-62%, and 49%, ci 38-5 ...200516402566
organochlorine and heavy-metal contaminants in wild mammals and birds of urbino-pesaro province, italy: an analytic overview for potential bioindicators.tissue samples from 56 bird and 11 mammal species of different trophic levels, collected from 1994 to 1995 from the urbino-pesaro area in the marche region of central italy, were analyzed for the presence of organochlorine compounds (polychlorinated biphenyls and p,p'-dde) and heavy metals (pb, cd, cr, and hg). results revealed interspecies differences in pollutant residue concentrations. a clear relationship between contaminant and trophic levels emerged depending on several factors specific to ...200616418892
low seroprevalence of antibodies to neospora caninum in wild canids in israel.the role of domestic dogs in the epidemiology of neospora caninum as well as the relationship between n. caninum infection of farm dogs and cattle were demonstrated, however, evidence is scarce regarding the role of wild canids in domestic animal neosporosis. the present study was undertaken to evaluate the role of wild canids in the epidemiology of bovine neosporosis in israel by analyzing the prevalence of antibodies to n. caninum in wild canids. sera samples were collected from 114 free rangi ...200616436314
pcr detection of neospora caninum, toxoplasma gondii and encephalitozoon cuniculi in brains of wild carnivores.neospora caninum, toxoplasma gondii and encephalitozoon cuniculi are important pathogens with affinity to the central nervous system of many animals. 240 brains of wild carnivores were examined by pcr-based diagnosis. the presence of n. caninum dna was confirmed in 4.61% (7/152) red foxes (vulpes vulpes). dna of t. gondii was found in 4.92% (3/61) martens (martes sp.) and in 1.32% (2/152) red foxes. dna of e. cuniculi was determined in 3.28% (2/61) martens and in one examined european otter (lut ...200616472923
toxoplasmosis: a serological survey in ontario wildlife.sera from seven species of wild animals in ontario were examined for antibody to toxoplasma gondii using the sabin-feldman dye test. of 158 sera tested, 53% of the red foxes (vulpes vulpes), 56% of the striped skunks (mephitis mephitis), 78% of the coyotes (canis latrans), 33% of the black bears (ursus americanus), 18% of the short tailed shrews (blarina brevicauda) and none of the field voles (microtus pennsylvanicus) had antibody. antibody to t. gondii was present in sera from wild animals cap ...197616502687
the susceptibility of five species of wild animals to experimental infection with leptospira grippotyphosa.five species of wild animals including opossums, didelphis marsupialis, striped skunks, mephitis mephitis, red, vulpes vulpes, and gray foxes, urocyon cinereoargenteus and raccoons, procyon lotor, were inoculated intraperitoneally with varying numbers of leptospira grippotyphosa, organisms. clinical signs were not detected, however, leptospiremia, leptospiruria and antibodies for the homologous organism, were demonstrated. lesions attributed to leptospirosis were observed in liver and kidney tis ...197016512129
pantropism of rabies virus in free-ranging rabid red fox vulpes fulva.the fluorescent antibody and mouse inoculation tests were employed to study the pantropism of rabies virus in tissue from free-ranging red fox (vulpes fulva) submitted for rabies diagnosis. viral antigen was found in various organs and tissues of the body. the distribution of antigen within tissues is discussed in the light of the pathogenesis and potential excretion of the virus.197016512165
geographic variation in body size: the effects of ambient temperature and precipitation.latitudinal trends in body size have been explained as a response to temperature- or water-related factors, which are predictors of primary production. we used the first principal component calculated from three body parameters (weight, body length and the greatest length of the skull) of a sample of mammals from israel and sinai to determine those species that vary in size geographically, and whether such variation is related to annual rainfall, average minimum january temperature and average m ...200616525785
epidemiological investigations on trichinella infections in farmed fur animals of estonia.farming of fur animals represents an important income in estonia. even though trichinella worms does not induce a symptomatic disease in carnivores, the carcasses of skinned animals can increase the biomass of the parasite in the environment, if they are not properly destroyed. the aim of the present survey was to study the prevalence of trichinella worms in farmed fur animals of estonia. of 281 muscle samples from blue foxes (alopex lagopus), silver foxes (vulpes vulpes fulva), minks (mustela v ...200616564631
chromosomes of the red fox. 194216578047
helminths of red foxes (vulpes vulpes) in denmark.an epidemiological study of helminths in 1040 red foxes collected from various localities in denmark during 1997-2002, revealed 21 helminth species at autopsy, including nine nematode species: capillaria plica (prevalence 80.5%), capillaria aerophila (74.1%), crenosoma vulpis (17.4%), angiostrongylus vasorum (48.6% from northern zealand (endemic area)), toxocara canis (59.4%), toxascaris leonina (0.6%), uncinaria stenocephala (68.6%), ancylostoma caninum (0.6%), and trichuris vulpis (0.5%); seve ...200616580775
remarkable findings concerning pbdes in the terrestrial top-predator red fox (vulpes vulpes).in the present study, we have analyzed muscle, liver, and adipose tissue of 33 red foxes from belgium for their content of polybrominated diphenyl ethers (pbdes). median sums of seven tri- to hepta-bdes (bde 28, bde 47, bde 99, bde 100, bde 153, bde 154, and bde 183) were 2.2, 2.4, and 3.4 ng/g lipid weight in adipose tissue, liver, and muscle, respectively. these levels were lower than those found in various species of voles and mice, the main prey species of the red fox. this is probably relat ...200616719094
the effect of sperm number on fertility in blue fox vixens (alopex lagopus ) artificially inseminated with frozen silver fox (vulpes vulpes ) semen.during the breeding seasons of 1989 and 1990, a total of 617 blue fox vixens aged 1 to 6 years (mean +/- sem, 2.6 +/- 0.1) were inseminated with frozen silver fox semen with either 150 million (n = 213, 1989 + 1990), 100 million (n=172, 1990), 75 million (n = 119, 1989) or 37.5 million (n = 113, 1989) spermatozoa per insemination. two intrauterine inseminations, each with an insemination volume of 1.0 ml, were performed at 24-hour intervals on the first and second days after maximum vaginal elec ...199216727071
the optimum time for single artificial insemination of blue fox vixens (alopex lagopus) with frozen-thawed semen from silver foxes (vulpes vulpes).during the breeding season of 1991 a total of 608 blue fox vixens aged 1 to 6 years (2.3 +/- 0.1 years, mean +/- sem) from 2 farms were artificially inseminated intrauterine once with frozen-thawed silver fox semen (1 ml dose containing a total of 150 million spermatozoa). the vixens were allocated to 3 different groups according to the time of insemination. vixens in group 1 (n = 203), group 2 (n = 198), and group 3 (n = 207) were inseminated on the first, second or third day after the peak val ...199216727185
in vitro culture of silver fox embryos.this experiment was designed to establish in vitro culture methods for silver fox embryos in order to develop the methods for evaluation of the post-thaw viability of frozen embryos in future studies. artificially inseminated silver fox females were killed humanely on predetermined days after insemination and oviducts and uteri were flushed for embryos. the embryos were cultured in modified tcm 199 or in the same medium supplemented with silver fox oviductal tissue suspension for varying periods ...199316727359
testosterone production in fetal testes of the silver fox.testosterone content was studied in fetal serum and testes of silver foxes during different periods of prenatal life. the first sign of testosterone synthesis in the testes could be detected starting from the 31st day of gestation by radioimmunoassay. subsequently its content sharply increased and showed maximum value on the 50th day. detectable serum testosterone level in male silver fox fetus was noted from the 40th day, its serum concentration increased in prenatal life, but only insignifican ...199416727535
cortisol production in fetal adrenals of the silver fox.the present study was designed to examine cortisol production by the silver fox fetal adrenals and their response to acth at different periods of prenatal life. serum levels of cortisol were determined on days 35, 40, 45 and 50 of gestation (term on day 52) in embryos of both sexes. cortisol content in adrenal tissue homogenates and its in vitro adrenal production were also investigated at the same time points. hormones were measured by ria. the levels of cortisol changed slightly during embryon ...199716728039
heterogeneity within the hemagglutinin genes of canine distemper virus (cdv) strains detected in italy.canine distemper virus (cdv) is a highly contagious viral pathogen causing lethal disease in dogs and other mammalians. a high degree of genetic variation is found between recent cdv strains and the old cdv isolates used in the vaccines and such genetic variation is regarded as a possible cause of the increasing number of cdv-related diseases in dogs. the h gene shows the greatest extent of genetic variation that allows for distinction of various lineages, according to a geographical pattern of ...200616730927
cyp2d-related metabolism in animals of the canoidea superfamily - species differences.cyp2d-related drug metabolism in liver microsomes from animals of the canoidea super family, i.e. mink (mustela vison), bears (ursus arctos), foxes (vulpes vulpes) and dogs, were investigated. propranolol, bunitrolol and imipramine, which are typically substrates of cyp2d subfamilies, were used in the experiment. all the animals of the canoidea superfamily that were tested lacked the ability to catalyse 7-hydroxylation of propranolol, which is one of the major metabolic pathways in rats. stereos ...200616755362
[black-silver fox vulpes vulpes male reproductive potential after longitudinal selection on domestic behavior]. 200916756099
behavioral styles in european rabbits: social interactions and responses to experimental stressors.the existence and consistency of individual behavioral types in response to challenging situations is of increasing interest in behavioral biology. in our study on european rabbits (oryctolagus cuniculus), we (1) investigated correlations between social behavior during early development and responses to experimental stressors during later life, and (2) tested for consistencies in these responses across different situations. for this, we observed juveniles living in field enclosures in early summ ...200616828816
[red fox (vulpes vulpes) as reservoir of parasites and source of zoonosis].red fox (vulpes vulpes) as reservoir of parasites and source of zoonosis. this review presents data from europe and poland on the prevalence of helminth and protozoan parasites in red foxes (vulpes vulpes). the most common nematodes were geohelminths: uncinaria stenocephala, toxocara canis and toxocara leonina. as concerning trichinella genus t. britovi was found more often than t. spiralis. among tapeworms the following species were recorded: mesocestoides lineatus, taenia sp., and echinococcus ...200516838621
control of rabies in slovenia.red foxes (vulpes vulpes) are the main reservoir of rabies in slovenia, whereas cases of rabies in other wildlife species occur sporadically. in 1995, a program of oral vaccination of wildlife in slovenia was initiated; baits with oral vaccine were distributed by air at a density of 20 baits/km(2). during 1995, when the oral vaccination program was started, 1,089 cases of rabies (including both wild and domestic animals) were reported. five years later (1999), only six positive animals were dete ...200616870876
spatial spreading of echinococcus multilocularis in red foxes (vulpes vulpes) across nation borders in western europe.the occurrence of the fox tapeworm echinococcus multilocularis in red foxes was studied in belgium and a neighbouring region in the netherlands. a total number of 1202 foxes were analysed (1018 in belgium and 184 in the netherlands) of which 179 were infected with e. multilocularis (164 in belgium and 15 in the netherlands). further, the spatial distribution of infection among sampled foxes was analysed with an ellipsoidal gradient, demonstrating a decreasing prevalence in northwestern direction ...200616872702
rabies in europe--trends and developments.rabies continues to be a major public health concern both in europe and worldwide. in the greater part of the european continent the disease has been eradicated, or its incidence drastically reduced, by oral vaccination of wildlife reservoirs. yet, 'hot spots' still remain in north-eastern and south-eastern europe with a high temporal variation of disease distribution in the previous decade. rabies is predominantly transmitted by the red fox; but in recent years, especially in eastern european c ...200616878461
the diversity of rabies virus in russia demonstrated by anti-nucleocapsid monoclonal antibody application and limited gene sequencing.a comparative analysis of anti-nucleocapsid monoclonal antibody (n-mab) reaction profiles and dna sequences was performed on 97 selected rabies virus (rabv) isolates from russia and neighbouring states. a panel of 73 n-mab from the wistar institute (philadelphia, pa, usa), veterinary laboratories agency (weybridge, uk) and p-41 (tübingen, germany) was used. the sequence of a 400 bp fragment of the n gene was generated for all available isolates and a phylogenetic analysis of the fragment was car ...200616878463
means used for terrestrial rabies elimination in france and policy for rabies surveillance in case of re-emergence.in france, the first case of wildlife rabies was detected in 1968, with the red fox (vulpes vulpes) as reservoir and vector of terrestrial rabies. the last case was reported at the end of 1998. the maximum infected area amounted to 140,000 km2 in 1989 with a record number of 4,213 infected wild and domestic animals. the contaminated areas included various landscapes such as low populated farming areas and low mountainous areas as well as very densely populated areas and industrial areas contiguo ...200616878468
rabies vaccination programme for red foxes (vulpes vulpes) and golden jackals (canis aureus) in israel (1999-2004).since 1956, red foxes (vulpes vulpes) and, to a lesser extent, golden jackals (canis aureus), have been the primary vectors maintaining endemic wildlife rabies in israel. starting in the autumn of 1998, oral rabies vaccination campaigns have been conducted in israel targeting these two wildlife species, with increasing yearly geographical extension. significant data have been accumulated from an area of approximately 5,200 km2 in northern israel. in the spring of 2003 the project was extended to ...200616878470
elimination of rabies in the czech republic.rabies in foxes was widespread in the territory of the czech republic after the second world war. the first three cases of fox rabies appeared in north and south bohemia in 1947. in the following year, a considerable increase in rabies incidence was reported and of the 146 cases, 106 (74 %) of the 117 wildlife cases were in foxes. rabies in foxes and other wild animals was verified in many border czech and moravian districts. sylvatic rabies was established in the czech republic and persisted th ...200616878471
cross reactive antigenicity in orally vaccinated foxes and raccoon dogs against european bat lyssavirus type 1 and 2.for the first time, the effectiveness of oral rabies vaccines against european bat lyssaviruses type 1 (eblv-1) and type 2 (eblv-2) by means of cross-neutralization assays was investigated. sera from orally vaccinated red foxes (vulpes vulpes) and raccoon dogs (nyctereutes procyonoides) with the street alabama dufferin (sad) b19 and sad p5/88 live-modified vaccine viruses were used to study the cross reactive antigenicity against cvs-11 (genotype 1), eblv-1 (genotype 5) and eblv-2 (genotype 6). ...200616878477
echinococcus multilocularis in north italy.alveolar echinococcosis is a zoonotic infection caused by the metacestode of the tapeworm echinococcus multilocularis. fox populations living in the alpine regions of italy had been considered free from this parasite until 2002, when two infected foxes were detected in bolzano province (trentino alto adige region) near austrian border. a modified nested pcr analysis was used to detect e. multilocularis dna in faecal samples belonging to red fox populations from five italian regions. a total of 5 ...200616881394
raccoon dog (nyctereutes procyonoides)--the new host of echinococcus multilocularis in poland.the alimentary canals of 9 species of domesticated or wild animals were examined for the presence of e. multilocularis. the tapeworm was found in 9 red foxes (vulpes vulpes) out of 155 examined (5.8%) and in 2 raccoon dogs (nyctereutes procyonoides) of 25 examined (8.0%). our studies proved that in poland the raccoon dog can also be infected with e. multilocularis.200216883704
prevalence of sarcocystis spp. in two subspecies of caribou (rangifer tarandus) in newfoundland and labrador, and foxes (vulpes vulpes), wolves (canis lupus), and husky dogs (canis familiaris) as potential definitive hosts.a study was conducted to determine the prevalence and geographical distribution of sarcocystis spp. infecting 2 subspecies of caribou (rangifer tarandus) inhabiting newfoundland and labrador and its potential definitive hosts. muscle samples of caribou were obtained, primarily from hunters, and feces of red foxes (vulpes vulpes) and wolves (canis lupus), from trappers, and husky dogs (canis familiaris), from owners. histological sections of muscle and flotation methods for feces were used for pa ...200616884021
[studies on the incidence of echinococcus multilocularis in red foxes (vulpes vulpes) in north-east, central and south of poland].during the year 1994-1997, a total number of 1042 red foxes orginating from the 33 provinces north-east, central and south of poland under special safety procautions for intestinal stages of echinococcus multilocularis using the mucosal-smer technique were examined. an average of 5.09% (53 foxes) from 10 provinces was infected. in those provinces, the prevalance varied between 1.19% to 35.71%. the infection varied from low to very high but mostly harboured gravid e. multilocularis parasites with ...199916886382
[the occurrence of intestinal nematodes in red foxes in the western pomerania].in the period from november 2004 to march 2005 the prevalence of gastro-intestinal nematodes of 165 foxes (vulpes vulpes l.) from the pomerania region was examined. these parasites were found in 66.1% of examined foxes. toxocara canis was the most frequent parasite and was observed in 33.9% of examined animals. toxascaris leonina was found in 1.2%, uncinaria stenocephala in 25.5%, ancylostoma caninum in 6.7 and trichuris vulpis in 10.3% of the examined foxes.200516913531
temporal distribution of ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna in hungary.a survey was carried out over a 4-year period to describe the temporal distribution of three 'anthropophilic' tick species, ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna in hungary. altogether 4658 adult ticks belonging to the three species were collected from 1931 red foxes (vulpes vulpes) killed in an area of about 70,000 km(2) representing all major climatic areas of the country. the seasonal activity of the three species was different. i. ricinus ticks were most active b ...200616919880
echinococcus multilocularis in the red fox vulpes vulpes from the east carpathian region of poland and the slovak republic.the occurrence of echinococcus multilocularis in the poland-slovak frontier zone of the east carpathian region was assessed, for comparison with that in adjacent regions in both countries. a total of 392 red foxes from poland and 427 red foxes from the slovak republic were examined from 2001 to 2004. significant differences in prevalences were observed in foxes captured from the borderland and adjacent zones in both countries. the mean prevalence of e. multilocularis in the polish borderland rea ...200616923266
[bromadiolone poisoning in foxes].bromadiolone is an anticoagulant rodenticide that inhibits the reactivation of vitamin k1 by the enzyme vitamin k1-epoxide reductase. the present case report originated from the application of bromadiolone against water voles (arvicola terrestris) in northeastern switzerland. at least 40 foxes (vulpes vulpes) were found dead after the inappropriate use of a bait that contained 0.02 % bromadiolone. anticoagulant rodenticide poisoning was suspected on the basis of the postmortem examination and su ...200616933704
persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against microsporum canis.to assist in evaluating serological test results from dead animals, 10 silver foxes (vulpes vulpes) and 10 blue foxes (alopex lagopus), 6 of each species previously vaccinated against and all challenged with microsporum canis, were blood sampled and euthanased. fox carcasses were stored at +10 degrees c, and autopsy was performed on days 0, 2, 4, 7, and 11 post mortem during which samples from blood and/or body fluid from the thoracic cavity were collected. antibodies against m. canis were measu ...200616987389
hepatopulmonary fistula caused by alveolar echinococcosis: report of a case.alveolar echinococcosis is a parasitosis endemic to red fox habitats in the northern hemisphere. the liver is the most commonly affected organ, followed by the lungs. we report the case of an elderly woman with hepatic alveolar echinococcosis (hae) complicated by a hepatopulmonary fistula. we performed a one-stage operation for the hepatic and pulmonary lesions through the transdiaphragmatic route via a laparotomy. we report this case to emphasize that the first-line treatment for a hepatopulmon ...200616998692
selective predation on utah prairie dogs.predation always affects demography and population dynamics, but removal of certain types of individuals is especially consequential. predators strike quickly and commonly avoid areas with human observers, however, and thereby make it difficult to document patterns of predation under natural conditions. at a colony of marked utah prairie dogs (cynomys parvidens), a high frequency of predation in 2005 provided an unusual opportunity to examine susceptibility of five types of individuals to predat ...200617004225
parasites of carnivorous mammals in białowieza primeval forest.although the parasitofauna of wild carnivorous mammals in poland is quite well recognized, there has been only one research carried on this issue so far in białowieza forest--the last lowland primeval forest of temperate zone in europe.200617007336
molecular epidemiology of trichinella spp. in three baltic countries: lithuania, latvia, and estonia.meat of domestic pigs and wild boars has been the significant source of emerged human trichinellosis in lithuania, latvia, and estonia over the past two decades. however, there is very little known on the occurrence of trichinella spp. in main wildlife reservoirs and its transmission in domestic and sylvatic cycles in these countries. the present study demonstrated considerably higher endemicity of trichinella spp. in main sylvatic reservoirs (28.9-42% in foxes (vulpes vulpes) and raccoon dogs ( ...200717013647
occurrence of giardia and cryptosporidium in norwegian red foxes (vulpes vulpes).faecal samples from 269 norwegian wild red foxes (vulpes vulpes) shot during the hunting season (october-april) in 2002-2004 were examined for the presence of giardia and cryptosporidium. cryptosporidium oocysts were detected in samples from 6 (2.2%) of the foxes, and giardia cysts in 13 (4.8%) of the foxes. the prevalence of giardia infection was significantly higher in juvenile male foxes than in adult male foxes, but no other significant differences between age and sex were found. no signific ...200717045400
prevalence of antibodies against toxoplasma gondii and neospora caninum in hungarian red foxes (vulpes vulpes).in the present study we have investigated the seroprevalence to the protozoan parasites toxoplasma gondii and neospora caninum in 337 red foxes (vulpes vulpes) from 16 out of 19 counties in hungary. the foxes were originally collected within a national vaccination program against rabies. antibodies to t. gondii were detected in as many as 228 (68%) of the foxes using a commercial direct agglutination test (dat). in an indirect iscom elisa, five foxes (1.5%) were positive for antibodies against n ...200717045742
trichinella nativa and trichinella t9 in the hokkaido island, japan.trichinella sp. muscle larvae were isolated from the thigh muscle of two red foxes (vulpes vulpes) captured in sapporo and otofuke, hokkaido, japan, in 2003. multiplex pcr designed for genotyping the genus trichinella revealed that the sapporo isolate showed a specific pattern to t. britovi complex (t. britovi, trichinella t8 and trichinella t9) and the otofuke isolate showed that to t. nativa. nucleotide sequences of a part of the mitochondrial cytochrome oxidase subunit i (coi) gene and intern ...200617049300
evaluation of a serological test (indirect elisa) for the diagnosis of sarcoptic mange in red foxes (vulpes vulpes).sarcoptic mange occurs in many parts of the world and is common in populations of domestic and wild canids, including red foxes (vulpes vulpes). in recent years, an indirect antibody enzyme-linked immunosorbent assay (elisa), with higher sensitivity and specificity than traditional diagnostic methods, has been successfully applied in the diagnosis of sarcoptic mange in dogs. the same elisa has also demonstrated specific antibodies to sarcoptes scabiei in experimentally infected red foxes. the ai ...200617083572
antibodies to mycobacterium bovis in wild carnivores from doñana national park (spain).we conducted a retrospective serologic survey for antibodies against the mpb70 protein of mycobacterium bovis in wild carnivores from doñana national park (southwestern spain). serum samples from 118 red foxes (vulpes vulpes), 39 iberian lynx (lynx pardinus), 31 eurasian badgers (meles meles), five egyptian mongoose (herpestes ichneumon), four european genet (genetta genetta), and one eurasian otter (lutra lutra) were analyzed using an indirect competitive enzyme-linked immunoassay. antibodies a ...200617092907
red fox (vulpes vulpes) a new host species for acanthocefalan moniliformis moniliformis (bremser, 1811) in poland.acanthocephalan moniliformis moniliformis is a parasite of rodents, rarely also reported from carnivorous mammals. one female specimen of this parasite has been found in the small intestine of red fox vulpes vulpes. it is the first report about this species invading the red fox in poland.200617120996
morphology of the lingual papillae in the raccoon dog and fox.the dorsal lingual surfaces of the raccoon dogs (nyctereutes procyonoides) and fox (vulpes vulpes japonica) were examined by scanning electron microscopical (sem) observations. the distribution and type of the lingual papillae found in the raccoon dog were similar to those in the fox. filiform, fungiform, foliate and vallate papillae were observed. the filiform papillae were distributed over the entire dosal surface of the tongue. each filiform papilla on the apical surface of the tongue had sev ...200617154050
rarity of a top predator triggers continent-wide collapse of mammal prey: dingoes and marsupials in australia.top predators in terrestrial ecosystems may limit populations of smaller predators that could otherwise become over abundant and cause declines and extinctions of some prey. it is therefore possible that top predators indirectly protect many species of prey from excessive predation. this effect has been demonstrated in some small-scale studies, but it is not known how general or important it is in maintaining prey biodiversity. during the last 150 years, australia has suffered the world's highes ...200717164197
evaluation of four serological techniques to determine the seroprevalence of neospora caninum in foxes (vulpes vulpes) and coyotes (canis latrans) on prince edward island, canada.the objectives of this study were (1) to evaluate the performance and agreement of serological assays (elisa, ifat, neospora caninum agglutination test and immunoblot) using reference sera and field sera from foxes and coyotes and (2) to estimate the n. caninum seroprevalence in foxes and coyotes on prince edward island, canada. with fox and coyote reference sera the test performance of the elisa, ifat and ib was excellent (100% sensitivity and specificity). nat showed a low sensitivity (50%). s ...200717223270
occurrence of cryptosporidium parvum and bacterial pathogens in faecal material in the red fox (vulpes vulpes) population. 200717225081
distribution and genetics of the color phases of the red fox in canada. 194517247144
serologic evidence of west nile virus exposure in north american mesopredators.sera from 936 mammalian mesopredators (virginia opossums, gray foxes, striped skunks, hooded skunks, raccoons, a bobcat, and a red fox) were collected during 2003 and 2004 in california, arizona, texas, louisiana, ohio, and wyoming and screened for flavivirus-specific antibodies by an epitope-blocking enzyme-linked immunosorbent assay (blocking elisa). serum samples positive for antibodies against flaviviruses were screened for west nile virus (wnv)-specific antibodies by blocking elisa and sele ...200717255248
population genetic analysis of serbian red foxes (vulpes vulpes) by means of mitochondrial control region sequences.a population genetic analysis based on sequences of the mitochondrial control region in 110 red foxes from five sampling localities in northern serbia was carried out. the analysis yielded nine different haplotypes. neither haplotype phylogeny nor their distribution was in accordance with the geographic location of the populations. in particular, the data failed to detect an unequivocal influence of the two big rivers, the danube and the tisza, separating the populations studied. population diff ...200717265184
canid genomics: mapping genes for behavior in the silver fox. 200717284673
a meiotic linkage map of the silver fox, aligned and compared to the canine genome.a meiotic linkage map is essential for mapping traits of interest and is often the first step toward understanding a cryptic genome. specific strains of silver fox (a variant of the red fox, vulpes vulpes), which segregate behavioral and morphological phenotypes, create a need for such a map. one such strain, selected for docility, exhibits friendly dog-like responses to humans, in contrast to another strain selected for aggression. development of a fox map is facilitated by the known cytogeneti ...200717284676
population dynamics and epidemiology of toxocara canis in danish red foxes.toxocara canis, an intestinal helminth of canids with zoonotic potential, was found in 618 (59%) of 1,040 red foxes (vulpes vulpes) collected from all danish provinces (1997-2002). the prevalence and average worm burden were significantly higher for cubs than older foxes and in males than in females. a multiple logistic regression demonstrated that the prevalence was influenced significantly by sex and age of foxes in addition to location, season, and year of collection. the highest prevalence a ...200617304794
neospora caninum-like oocysts observed in feces of free-ranging red foxes (vulpes vulpes) and coyotes (canis latrans).the aim of this study was to examine the feces of free-ranging foxes and coyotes for the presence of neospora caninum oocysts. feces were collected from 271 foxes and 185 coyotes in the canadian province of prince edward island, processed by sucrose flotation, and examined by light microscopy for the presence of coccidian oocysts. in 2 fox and 2 coyote samples, oocysts morphologically and morphometrically similar to oocysts of n. caninum were observed. dna was extracted from these samples and su ...200617304805
trophic control of mesopredators in terrestrial ecosystems: top-down or bottom-up?it has been argued that widespread extinctions of top predators have changed terrestrial ecosystem structures through mesopredator release, where increased abundances of medium-sized predators have detrimental effects on prey communities. this top-down concept has received much attention within conservation biology, but few studies have demonstrated the phenomenon. the concept has been criticized since alternative explanations involving bottom-up impacts from bioclimatic effects on ecosystem pro ...200717305803
polymorphism and chromosomal location of the mc4r (melanocortin-4 receptor) gene in the dog and red fox.the melanocortin-4 receptor (mc4r) is expressed in the hypothalamus and regulates energy intake and body weight. in silico screening of the canine chromosome 1 sequence and a comparison with the porcine mc4r sequence by blast were performed. the nucleotide sequence of the whole coding region and 3'- and 5'-flanking regions of the dog (1214 bp) and red fox (1177 bp) mc4r gene was established and high conservation of the nucleotide sequences was revealed (99%). five sets of pcr primers were design ...200717306938
epidemiology, histopathology, and muscle distribution of trichinella t9 in feral raccoons (procyon lotor) and wildlife of japan.the prevalences of trichinella t9 in trapped raccoons (procyon lotor) and several other potential mammalian reservoirs in hokkaido, wakayama, and nagasaki prefectures were investigated. muscle samples were collected from 2003 to 2006 from 1,080 raccoons, 113 raccoon dogs including 2 species (nyctereutes procyonoides albus and n. p. viverrinus), 41 wild boars (sus scrofa leucomystax), 14 martens (martes melampus), 10 badgers (meles meles), 5 siberian weasels (martes sibirica coreana), 7 mink (mus ...200717310398
biomagnification of pbdes in three small terrestrial food chains.eight polybrominated diphenyl ethers (pbde) congeners (iupac nos. 28, 47, 99, 100, 153, 154, 183, and 209) were measured in passerines (great tits--parus major), wood mice (apodemus sylvaticus), and bank voles (clethrionomys glareolus). these data were combined with previously obtained data on pbdes in common buzzards (buteo buteo), sparrowhawks (accipiter nisus), and red fox (vulpes vulpes). this enabled estimation of the biomagnification potential of pbdes in the following three terrestrial fo ...200717310700
echinococcus multilocularis and toxocara canis in urban red foxes (vulpes vulpes) in brussels, belgium.during the last decades, european red foxes (vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. in urban areas, risks of zoonoses transmission are likely to increase as a result of a higher rate of intra- and inter-species contacts. foxes occur on 35% of the brussels-capital region area and local densities reach up to 4 family groups per square kilometre. according to the directive 2003/99/ecc, a first survey fo ...200717324480
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