Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview. | hemostatic and adhesive agents date back to world war ii, when homologous fibrin sealant came onto scene. considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. its components were a serine protease (a thrombin-like enzyme) extracted from the venom of crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of bubalus bubalis buffaloes. this new bioproduct has been used as ... | 2017 | 28396682 |
| no production and potassium channels activation induced by crotalus durissus cascavella underlie mesenteric artery relaxation. | animal toxins are natural resources for pharmacological studies. the venom of crotalus durissus cascavella (c.d. cascavella) may be a source in the bio-prospecting of new anti-hypertensive agents. the aim of this study was to investigate vascular effects of the venom of c.d. cascavella in normotensive rats. studies were performed using isolated mesenteric artery segments and aortic endothelial cells. the cumulative administration of the venom of c.d. cascavella (0.001-30 μg/ml) on phenylephrine ... | 2017 | 28428070 |
| opioid neurotransmission modulates defensive behavior and fear-induced antinociception in dangerous environments. | the effects of endogenous opioid peptide antagonists on panic-related responses are controversial. using elevated mazes and a prey-versus-predator paradigm, we investigated the involvement of the endogenous opioid peptide-mediated system in the modulation of anxiety- and panic attack-induced responses and innate fear-induced antinociception in the present work. wistar rats were intraperitoneally pretreated with either physiological saline or naloxone at different doses and were subjected to eith ... | 2017 | 28457818 |
| microcomputed tomographic, morphometric, and histopathologic assessment of congenital bone malformations in two neotropical viperids. | congenital malformations have been reported in all classes of vertebrates and may be a determinant of life span and survival. in reptiles, the incidence of congenital malformations can be associated with genetic and environmental causes, including pollution. the characterization of pathological processes involved in the development of congenital malformations of bone in snakes is rare in the literature, but is of great relevance in the field of reptile conservation and environmental health. we d ... | 2017 | 28463628 |
| antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine. | snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. to achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. in brazil, antivenom against south american rattlesnake (crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the brazilian ministry of health, anvisa. however, each ... | 2017 | 28507562 |
| commentary on: "vascular distensibilities have minor effects on intracardiac shunt patterns in reptiles" by filogonio et al. (2017). | the recent study by filogonio et al. (2017) suggested that net cardiac shunt patterns in two species of reptiles (trachemys scripta and crotalus durissus) were not significantly influenced by the vascular distensibilities of the systemic and pulmonary vasculatures. this is in contrast to a previously published study (hillman et al., 2014) in the toad (rhinella marina) in which net cardiac shunts were predicted primarily by the physical properties of vascular distensibility rather than physiologi ... | 2017 | 28546068 |
| reply to the commentary by hillman et al. on: "vascular distensibilities have minor effects on intracardiac shunt patterns in reptiles" by filogonio et al. (2017). | our meta-analysis (filogonio et al., 2017) on central vascular blood flows in a snake (crotalus durissus) and a turtle (trachemys scripta) was motivated by hillman et al.'s (2014) analysis on amphibians to investigate whether cardiac shunt patterns depend on cardiac output and vascular distensibilities. in contrast to hillman et al. (2014), we did not uncover a general trend that supports the notion that cardiac shunts in reptiles are dictated by vascular distensibilities. in addition to our res ... | 2017 | 28550946 |
| crotoxin stimulates an m1 activation profile in murine macrophages during leishmania amazonensis infection. | american tegumentary leishmaniasis is caused by different species of leishmania. this protozoan employs several mechanisms to subvert the microbicidal activity of macrophages and, given the limited efficacy of current therapies, the development of alternative treatments is essential. animal venoms are known to exhibit a variety of pharmacological activities, including antiparasitic effects. crotoxin (ctx) is the main component of crotalus durissus terrificus venom, and it has several biological ... | 2017 | 28641584 |
| a snake venom group iia pla2 with immunomodulatory activity induces formation of lipid droplets containing 15-d-pgj2 in macrophages. | crotoxin b (cb) is a catalytically active group iia spla2 from crotalus durissus terrificus snake venom. in contrast to most giia spla2s, cb exhibits anti-inflammatory effects, including the ability to inhibit leukocyte functions. lipid droplets (lds) are lipid-rich organelles associated with inflammation and recognized as a site for the synthesis of inflammatory lipid mediators. here, the ability of cb to induce formation of lds and the mechanisms involved in this effect were investigated in is ... | 2017 | 28642580 |
| origin and characterization of small membranous vesicles present in the venom of crotalus durissus terrificus. | small membranous vesicles are small closed fragments of membrane. they are released from multivesicular bodies (exosomes) or shed from the surface membrane (microvesicles). they contains various bioactive molecules and their molecular composition varies depending on their cellular origin. small membranous vesicles have been identified in snake venoms, but the origin of these small membranous vesicles in the venom is controversial. the aim of this study was to verify the origin of the small membr ... | 2017 | 28668562 |
| crotoxin, a rattlesnake toxin, down-modulates functions of bone marrow neutrophils and impairs the syk-gtpase pathway. | neutrophils have a critical role in the innate immune response; these cells represent the primary line of defense against invading pathogens or tissue injury. crotoxin (ctx), the major toxin of the south american rattlesnake (crotalus durissus terrificus) venom, presents longstanding anti-inflammatory properties, inhibiting neutrophil migration and phagocytosis by peritoneal neutrophils for 14 days. herein, to elucidate these sustained inhibitory effects induced by ctx, we performed in vitro and ... | 2017 | 28688804 |
| phospholipase a2inhibitor from crotalus durissus terrificus rattlesnake: effects on human peripheral blood mononuclear cells and human neutrophils cells. | crotalus neutralizing factor (cnf) is an inhibitor of phospholipase a2(pla2), present in the blood plasma of crotalus durissus terrificus snake. this inhibitor neutralizes the lethal and enzymatic activity of crotoxin, the main neurotoxin from this venom. in this study, we investigated the effects of cnf on the functionality of human peripheral blood mononuclear cells (pbmcs) and human neutrophils. the following parameters were evaluated: viability and proliferation, chemotaxis, cytokines and lt ... | 2017 | 28743568 |
| the complementarity-determining region sequences in igy antivenom hypervariable regions. | the data presented in this article are related to the research article entitled "development of igy antibodies against anti-snake toxins endowed with highly lethal neutralizing activity" (da rocha et al., 2017) [1]. complementarity-determining region (cdr) sequences are variable antibody (ab) sequences that respond with specificity, duration and strength to identify and bind to antigen (ag) epitopes. b lymphocytes isolated from hens immunized withbitis arietans(ba) and anti-crotalus durissus ter ... | 2017 | 28748206 |
| pulmonary mechanic and lung histology induced by crotalus durissus cascavella snake venom. | this study have analyzed the pulmonary function in an experimental model of acute lung injury, induced by the crotalus durissus cascavella venom (c. d. cascavella) (3.0 μg/kg - i.p), in pulmonary mechanic and histology at 1 h, 3 h, 6 h, 12 h and 24 h after inoculation. the c. d. cascavella venom led to an increase in newtonian resistance (rn), tissue resistance (g) and tissue elastance (h) in all groups when compared to the control, particularly at 12 h and 24 h. the histeresivity (η) increased ... | 2017 | 28760509 |
| antitumoral effects of γcdcpli, a pla2 inhibitor from crotalus durissus collilineatus via pi3k/akt pathway on mda-mb-231 breast cancer cell. | phospholipases a2 (pla2s) overexpression is closely associated with the malignant potential of breast cancers. here, we showed for the first the antitumoral effects of γcdcpli, a pla2 inhibitor from crotalus durissus collilineatus via pi3k/akt pathway on mda-mb-231 cell. firstly, γcdcpli was more cytotoxic to mda-mb-231 breast cancer cells than other cell lines (mcf-7, hela, pc3 and a549) and did not affect the viability of non-tumorigenic breast cell (mcf 10a). in addition, γcdcpli induced modu ... | 2017 | 28765552 |
| tetrodotoxin-insensitive electrical field stimulation-induced contractions on crotalus durissus terrificus corpus cavernosum. | reptiles are the first amniotes to develop an intromitent penis, however until now the mechanisms involved in the electrical field stimulation-induced contraction on corpora cavernosa isolated from crotalus durissus terrificus were not investigated. crotalus and rabbit corpora cavernosa were mounted in 10 ml organ baths for isometric tension recording. electrical field stimulation (efs)-induced contractions were performed in presence/absence of phentolamine (10 μm), guanethidine (30 μm), tetrodo ... | 2017 | 28837636 |
| neuromuscular paralysis by the basic phospholipase a2subunit of crotoxin from crotalus durissus terrificus snake venom needs its acid chaperone to concurrently inhibit acetylcholine release and produce muscle blockage. | crotoxin (ctx), a heterodimeric phospholipase a2(pla2) neurotoxin from crotalus durissus terrificus snake venom, promotes irreversible blockade of neuromuscular transmission. indirect electrophysiological evidence suggests that ctx exerts a primary inhibitory action on transmitter exocytosis, yet contribution of a postsynaptic action of the toxin resulting from nicotinic receptor desensitization cannot be excluded. here, we examined the blocking effect of ctx on nerve-evoked transmitter release ... | 2017 | 28867438 |
| discovery of human scfvs that cross-neutralize the toxic effects of b. jararacussu and c. d. terrificus venoms. | accidents involving venomous snakes are a public health problem worldwide, causing a large number of deaths per year. in brazil, the majority of accidents are caused by the bothrops and crotalus genera, which are responsible for approximately 80% of severe envenoming cases. the cross-neutralization of snake venoms by antibodies is an important issue for development of more effective treatments. our group has previously reported the construction of human monoclonal antibody fragments towards both ... | 2018 | 28887121 |
| biophysical and biological properties of small linear peptides derived from crotamine, a cationic antimicrobial/antitumoral toxin with cell penetrating and cargo delivery abilities. | crotamine is a natural polypeptide from snake venom which delivers nucleic acid molecules into cells, besides having pronounced affinity for negatively charged membranes and antifungal activity. we previously demonstrated that crotamine derived short linear peptides were not very effective as antifungal, although the non-structured recombinant crotamine was overridingly more potent compared to the native structured crotamine. aiming to identify the features necessary for the antifungal activity ... | 2017 | 28888370 |
| non-clinical studies for evaluation of 8-c-rhamnosyl apigenin purified from peperomia obtusifolia against acute edema. | compound 8-c-rhamnosyl apigenin (8cr) induced a moderate reduction in the enzymatic activity of secretory phospholipase a2 (spla2) fromcrotalus durissus terrificusand cytosolic phospholipase a2 (cpla2), but the compound also significantly inhibited the enzymatic activity of the enzyme cyclooxygenase. in vitro assays showed that the compound induced a slight change in the secondary structure of spla2 fromcrotalus durissus terrificussnake venom. in vivo assays were divided into two steps. in the f ... | 2017 | 28906474 |
| neuromuscular effect of venoms from adults and juveniles of crotalus durissus cumanensis (humboldt, 1811) from guajira, colombia. | a toxinological study was performed to compare the neuromuscular effect of venom from adult and juvenile specimens from crotalus durissus cumanensis from guajira, colombia. both venoms exhibited neurotoxic activity in chick biventer cervicis nerve-muscle preparation. in addition, venom from juveniles was faster than adults to produce a neuromuscular blockade. in the contrary to the venom from juvenile's, at high doses, adult's venom affected the ach and kcl contractures, indicating a myotoxic ef ... | 2017 | 28978413 |
| pancreatic and snake venom presynaptically active phospholipases a2 inhibit nicotinic acetylcholine receptors. | phospholipases a2 (pla2s) are enzymes found throughout the animal kingdom. they hydrolyze phospholipids in the sn-2 position producing lysophospholipids and unsaturated fatty acids, agents that can damage membranes. pla2s from snake venoms have numerous toxic effects, not all of which can be explained by phospholipid hydrolysis, and each enzyme has a specific effect. we have earlier demonstrated the capability of several snake venom pla2s with different enzymatic, cytotoxic, anticoagulant and an ... | 2017 | 29023569 |
| antivenom against crotalus durissus terrificus venom: immunochemical reactivity and experimental neutralizing capacity. | the venom of crotalus durissus terrificus produces a neurotoxic and myotoxic syndrome that can lead to the death. specific antivenom is the only treatment to neutralize the toxicity of the venom and the precocity in applying the antivenom is crucial for the efficiency of the treatment. we studied the variation of the immunochemical reactivity and neutralizing capacity of the specific antivenom on this venom in pre-incubation and rescue experiments, at different times. elisa titers increased with ... | 2017 | 29042310 |
| multiple effects of toxins isolated from crotalus durissus terrificus on the hepatitis c virus life cycle. | hepatitis c virus (hcv) is one of the main causes of liver disease and transplantation worldwide. current therapy is expensive, presents additional side effects and viral resistance has been described. therefore, studies for developing more efficient antivirals against hcv are needed. compounds isolated from animal venoms have shown antiviral activity against some viruses such as dengue virus, yellow fever virus and measles virus. in this study, we evaluated the effect of the complex crotoxin (c ... | 2017 | 29141010 |
| antichagasic effect of crotalicidin, a cathelicidin-like vipericidin, found in crotalus durissus terrificus rattlesnake's venom gland. | cathelicidins are antimicrobial peptides produced by humans and animals in response to various pathogenic microbes. crotalicidin (ctn), a cathelicidin-related vipericidin from the south american crotalus durissus terrificus rattlesnake's venom gland, and its fragments have demonstrated antimicrobial and antifungal activity, similarly to human cathelicidin ll-37. in order to provide templates for the development of modern trypanocidal agents, the present study evaluated the antichagasic effect of ... | 2017 | 29208061 |
| crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions. | classically, crotalus durissus terrificus (cdt) venom can be described, according to chromatographic criteria, as a simple venom, composed of four major toxins, namely: gyroxin, crotamine, crotoxin and convulxin. crotoxin is a non-covalent heterodimeric neurotoxin constituted of two subunits: an active phospholipase a2 and a chaperone protein, termed crotapotin. this molecule is composed of three peptide chains connected by seven disulfide bridges. naturally occurring variants/isoforms of either ... | 2017 | 29209361 |
| successful reconstruction of whole mitochondrial genomes from ancient central america and mexico. | the northern and southern peripheries of ancient mesoamerica are poorly understood. there has been speculation over whether borderland cultures such as greater nicoya and casas grandes represent mesoamerican outposts in the isthmo-colombian area and the greater southwest, respectively. poor ancient dna preservation in these regions challenged previous attempts to resolve these questions using conventional genetic techniques. we apply advanced in-solution mitogenome capture and high-throughput se ... | 2017 | 29273718 |
| global proteomic and functional analysis of crotalus durissus collilineatus individual venom variation and its impact on envenoming. | individual variations studies are important to understand the snakebite envenoming and to improve the antivenom production and its effectiveness. in this way, the objective of this study was a comparative analysis of intraspecific variation in the venom composition of 22 crotalus durissus collilineatus specimens through proteomic techniques. venoms were fractionated by rp-fplc, and analyzed by sds-page and mass spectrometry. although similar, chromatographic and electrophoretic profiles showed s ... | 2019 | 29462664 |
| characterization of the venom of c. d. cumanesis of colombia: proteomic analysis and antivenomic study. | the colombian rattlesnake crotalus durissus cumanensis is distributed in three geographic zones of the country: the atlantic coast, the upper valley of the magdalena river, and the eastern plains of the colombian orinoquía. its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. identification and analysis of c. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which ... | 2018 | 29462980 |
| new records of helminths in reptiles from five states of brazil. | forty five specimens representing nine species of reptile (salvator merianae, enyalius bilineatus, amphisbaena alba, xenopholis undulatus, chironius fuscus, helicops angulatus, chironius flavolineatus, erythrolamprus viridis and crotalus durissus) collected in five brazilian states were examined for helminths. twelve helminth species were found as follow: nine nematoda (physaloptera tupinambae, strongyluris oscari, paracapillaria sp., dracunculus brasiliensis, physaloptera liophis, serpentirhabi ... | 2018 | 29489929 |
| protocol to obtain targeted transcript sequence data from snake venom samples collected in the colombian field. | snake venoms are a mixture of different molecules that can be used in the design of drugs for various diseases. the study of these venoms has relied on strategies that use complete venom extracted from animals in captivity or from venom glands that require the sacrifice of the animals. colombia, a country with political and geographical conflicts has difficult access to certain regions. a strategy that can prevent the sacrifice of animals and could allow the study of samples collected in the fie ... | 2018 | 29571682 |
| mechanistic insights into functional characteristics of native crotamine. | the chemical composition of snake venoms is a complex mixture of proteins and peptides that can be pharmacologically active. crotamine, a cell-penetrating peptide, has been described to have antimicrobial properties and it exerts its effects by interacting selectively with different structures, inducing changes in the ion flow pattern and cellular responses. however, its real therapeutic potential is not yet fully known. bearing in mind that crotamine is a promising molecule in therapeutics, thi ... | 2018 | 29574214 |
| camelid single-domain antibodies (vhhs) against crotoxin: a basis for developing modular building blocks for the enhancement of treatment or diagnosis of crotalic envenoming. | toxic effects triggered by crotalic envenoming are mainly related to crotoxin (ctx), composed of a phospholipase a₂ (cb) and a subunit with no toxic activity (ca). camelids produce immunoglobulins g devoid of light chains, in which the antigen recognition domain is called vhh. given their unique characteristics, vhhs were selected using phage display against ctx from crotalus durissus terrificus. after three rounds of biopanning, four sequence profiles for cb (kf498602, kf498603, kf498604, and k ... | 2018 | 29596324 |
| a functional and thromboelastometric-based micromethod for assessing crotoxin anticoagulant activity and antiserum relative potency against crotalus durissus terrificus venom. | the assessment of the capacity of antivenoms to neutralize the lethal activity of snake venoms still relies on traditional rodent in vivo lethality assay. ed50 and ld50 assays require large quantities of venoms and antivenoms, and besides leading to animal suffering. therefore, in vitro tests should be introduced for assessing antivenom neutralizing capacity in intermediary steps of antivenom production. this task is facilitated when one key lethal toxin is identified. a good example is crotoxin ... | 2018 | 29654870 |
| electrical field stimulation-induced contractions on pantherophis guttatus corpora cavernosa and aortae. | a tetrodotoxin (ttx)-resistant mechanism is responsible for the electrical field stimulation (efs)-induced contractions and relaxations of crotalus durissus terrificus corpora cavernosa. here it was investigated whether this mechanism also occurs in corpora cavernosa and aortae of the non-venomous snake pantherophis guttatus corpora cavernosa and aortae. corpora cavernosa and aortic rings isolated from pantherophis guttatus snake were mounted in organ bath system for isometric tension recording. ... | 2018 | 29672643 |
| vertebrates as a bactericidal agent. | in brazil, although a large number of animals are used in traditional medicine (at least 354 species), information about their biological activities is scarce. in this context, the objective of this study was to evaluate the bactericidal potential of zootherapeutic by-products from animals used in brazilian traditional medicine and discuss the ecological and cultural consequences of such practices. the species analyzed were: tupinambis merianae (skin), iguana iguana (skin and body fat), crotalus ... | 2018 | 29922961 |
| crotoxin isolated from crotalus durissus terrificus venom modulates the functional activity of dendritic cells via formyl peptide receptors. | the crotalus durissus terrificus rattlesnake venom, its main toxin, crotoxin (ctx), and its crotapotin (ca) and phospholipase a2 (cb) subunits modulate the immune system. formyl peptide receptors (fprs) and lipoxin a4 (lxa4) are involved in ctx's effect on macrophages and neutrophils. dendritic cells (dcs) are plasticity cells involved in the induction of adaptive immunity and tolerance maintenance. therefore, we evaluated the effect of ctx, ca or cb on the maturation of dcs derived from murine ... | 2018 | 29967803 |
| edema induced by a crotalus durissus terrificus venom serine protease (cdtsp 2) involves the par pathway and pkc and plc activation. | snake venom serine proteases (svsps) represent an essential group of enzymatic toxins involved in several pathophysiological effects on blood homeostasis. some findings suggest the involvement of this class of enzymatic toxins in inflammation. in this paper, we purified and isolated a new gyroxin isoform from the crotalus durissus terrificus (cdt) venom, designated as cdtsp 2, which showed significant proinflammatory effects in a murine model. in addition, we performed several studies to elucida ... | 2018 | 30111691 |
| crotalus durissus sp. rattlesnake venom induces toxic injury in mouse sperm. | accidents involving snakebites constitute a serious public health problem in many regions around the world. however, there are no study about a possible action of snake venom on the reproductive system. herein we show that crotalus durissus sp. (linnaeus) rattlesnake venom (25 μg/kg of body weight) affected chromatin condensation, and increased the number of sperm with abnormal morphology and the sperm count. in conclusion, besides the known hazards of the c. durissus sp. venom to animal health, ... | 2018 | 30149042 |
| essential oil from lippia origanoides (verbenaceae): haemostasis and enzymes activity alterations. | the search for natural inhibitors of snake venom toxins is essential to supplement or even replace the serum therapy. the aim of this work was to evaluate the pharmacological properties of essential oil from lippia origanoides kunth. (verbenaceae). | 2019 | 30160215 |
| electrical field-induced contractions on crotalus durissus terrificus and bothrops jararaca aortae are caused by endothelium-derived catecholamine. | endothelium is the main source of catecholamine release in the electrical-field stimulation (efs)-induced aortic contractions of the non- venomous snake panterophis guttatus. however, adrenergic vasomotor control in venomous snakes such as crotalus durissus terrificus and bothrops jararaca has not yet been investigated. crotalus and bothrops aortic rings were mounted in an organ bath system. efs-induced aortae contractions were performed in the presence and absence of guanethidine (30 μm), phent ... | 2018 | 30199552 |
| cdna cloning, heterologous expression, protein folding and immunogenic properties of a phospholipase a2 from bothrops ammodytoides venom. | a mrna transcript that codes for a phospholipase (pla2) was isolated from a single venom gland of the bothrops ammodytoides viper. the pla2 transcript was cloned onto a pcr®2.1-topo vector and subsequently expressed heterologously in the e. coli strain m15, using the pqe30 vector. the recombinant phospholipase was named rbampla2_1, and is composed of an n-terminal fusion protein of 16 residues, along with 122 residues from the mature protein that includes 14 cysteines that form 7 disulfide bonds ... | 2019 | 30205154 |
| molecular characterization and identification of hepatozoon species miller, 1908 (apicomplexa: adeleina: hepatozoidae) in captive snakes from brazil. | species of hepatozoon are parasites frequently recorded in snakes. the species identification of this genus was based mostly on the gametocyte morphology and morphometric calculations. for more reliable results, molecular characterization, an initial step for the correct identification of hepatozoon species, has been used. this study aimed to determine the prevalence and identification of hepatozoon species in captive snakes from brazil. to that end, morphological, morphometric, and molecular da ... | 2018 | 30267181 |
| antimicrobial and proinflammatory effects of two vipericidins. | hospital infections allied to bacterial resistance to antibiotics have become a major worldwide problem. in this context, antimicrobial peptides (amps) are presented as an alternative in the control of these resistant organisms. besides antimicrobial effects, these molecules play a crucial role in immunity by acting as immunomodulators. these peptides can activate inflammatory cells to produce pro- and anti-inflammatory mediators. in this study we will show the activity against multi-drug resist ... | 2018 | 30269027 |
| in-depth venome of the brazilian rattlesnake crotalus durissus terrificus: an integrative approach combining its venom gland transcriptome and venom proteome. | snake venoms are complex mixtures mainly composed of proteins and small peptides. crotoxin is one of the most studied components from crotalus venoms, but many other components are less known due to their low abundance. the venome of crotalus durissus terrificus, the most lethal brazilian snake, was investigated by combining its venom gland transcriptome and proteome to create a holistic database of venom compounds unraveling novel toxins. we constructed a cdna library from c. d. terrificus veno ... | 2018 | 30270628 |
| a biotechnological approach to immunotherapy: antivenom against crotalus durissus cascavella snake venom produced from biodegradable nanoparticles. | snakebite envenoming is a tropical disease neglected worldwide. in brazil, the crotalus durissus cascavella (cdc) snake belongs to a genus with venom of highest lethality. a search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. this approach proposed to produce small and narrow-sized cationic cdc venom-loaded chitosan nanoparticles (chnp) able to induce antibody response against the cdc venom. the ionic gelation method induced the formatio ... | 2018 | 30287370 |
| fruit bagasse phytochemicals from malpighia emarginata rich in enzymatic inhibitor with modulatory action on hemostatic processes. | agro-industrial wastes are promising sources of phytochemicals for the development of products to be used in health promotion and maintenance. in this study, extracts from acerola bagasse (ab) were characterized by hplc, and evaluated according to its modulatory action on phospholipases a2 and proteases involved in processes such as inflammation and blood clotting. snake venoms were used as biological tools once they have high functional and structural homology between their enzymes and human en ... | 2018 | 30334251 |
| cell migration inhibition activity of a non-rgd disintegrin from crotalus durissus collilineatus venom. | in recent decades, snake venom disintegrins have received special attention due to their potential use in anticancer therapy. disintegrins are small and cysteine-rich proteins present in snake venoms and can interact with specific integrins to inhibit their activities in cell-cell and cell-ecm interactions. these molecules, known to inhibit platelet aggregation, are also capable of interacting with certain cancer-related integrins, and may interfere in important processes involved in carcinogene ... | 2018 | 30377432 |
| crotoxin from crotalus durissus terrificus venom: in vitro cytotoxic activity of a heterodimeric phospholipase a2 on human cancer-derived cell lines. | crotoxin (ctx), a heterodimeric phospholipase present in venom of snakes of the genus crotalus, has demonstrated a broad spectrum of pharmacological properties, such as antimicrobial, hemostatic, and antitumoral. however, the precise mechanism of its cytotoxicity and antitumoral properties remains to be determined. therefore, in the present study, we isolated crotoxin (f1 ctx) through two steps deae-sepharose and heparin-sepharose ff chromatography. the c-terminal sequence of the a- and b-chain ... | 2018 | 30395843 |
| the endogenous opioid system modulates defensive behavior evoked by crotalus durissus terrificus: panicolytic-like effect of intracollicular non-selective opioid receptors blockade. | there is a controversy regarding the key role played by opioid peptide neurotransmission in the modulation of panic-attack-related responses. | 2019 | 30407114 |
| histopathological findings in the viscera of malformed neotropical viperids. | congenital malformations (cms) or developmental abnormalities are pathological processes defined as morphological defects that originate during embryonic stages. a wide variety of cms is observed in reptiles, most of which are incompatible with normal life. characterization of cms and their pathogenesis are of great importance for the conservation of reptiles, including neotropical viperids (nvs). external cms are often reported in these species, but little is known about concomitant microscopic ... | 2019 | 30503577 |
| antivenomic approach of different crotalus durissus collilineatus venoms. | our group has previously performed a proteomic study verifying that individual variations can occur among crotalus durissus collilineatus venoms. these variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. in this way, this study aimed to evaluate the brazilian anticrotalic serum capacity in recognizing twenty-two crotalus durissus collilineatus venoms, as well as their fractions. | 2018 | 30534148 |
| immunotherapeutic potential of crotoxin: anti-inflammatory and immunosuppressive properties. | for the past 80 years, crotoxin has become one of the most investigated isolated toxins from snake venoms, partially due to its major role as the main toxic component in the venom of the south american rattlesnake crotalus durissus terrificus. however, in the past decades, progressive studies have led researchers to shift their focus on crotoxin, opening novel perspectives and applications as a therapeutic approach. although this toxin acts on a wide variety of biological events, the modulation ... | 2018 | 30564276 |
| rattlesnakes bites in the brazilian amazon: clinical epidemiology, spatial distribution and ecological determinants. | crotalus bites are considered a public health problem especially in latin america. this study was performed to describe the epidemiology, spatial distribution and environmental determinants of crotalus durissus bites in the brazilian amazon. crotalus durissus envenomings official database included cases reported from 2010 to 2015. a total of 70,816 snakebites were recorded in the amazon region, 3058 (4.3%) cases being classified as crotalid, with a mean incidence rate of 11.1/100,000 inhabitants ... | 2019 | 30579811 |
| intradermal application of crotamine induces inflammatory and immunological changes in vivo. | crotamine is a single-chain polypeptide with cell-penetrating properties, which is considered a promising molecule for clinical use. nevertheless, its biosafety data are still scarce. herein, we assessed the in vivo proinflammatory properties of crotamine, including its local effect and systemic serum parameters. sixty male wistar rats were intradermically injected with 200, 400 and 800 µg crotamine and analyzed after 1, 3 and 7 days. local effect of crotamine was assessed by determination of mp ... | 2019 | 30646542 |
| transected tendon treated with a new fibrin sealant alone or associated with adipose-derived stem cells. | tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. this study seeks to use a new fibrin sealant (fs) derived from the venom of crotalus durissus terrificus, a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (asc) for application in tendons injuries, considered a common and serious orthopedic problem. lewis rats had tendons ... | 2019 | 30654437 |
| panicolytic-like effect of µ1-opioid receptor blockade in the inferior colliculus of prey threatened by crotalus durissus terrificus pit vipers. | the endogenous opioid peptide system has been implicated in the neural modulation of fear and anxiety organised by the dorsal midbrain. furthermore, previous results indicate a fundamental role played by inferior colliculus (ic) opioid mechanisms during the expression of defensive behaviours, but the involvement of the ic µ1-opioid receptor in the modulation of anxiety- and panic attack-related behaviours remains unclear. using a prey-versus-snake confrontation paradigm, we sought to investigate ... | 2019 | 30663473 |
| expression, purification and virucidal activity of two recombinant isoforms of phospholipase a2 from crotalus durissus terrificus venom. | the global emergence and re-emergence of arthropod-borne viruses (arboviruses) over the past four decades have become a public health crisis of international concern, especially in tropical and subtropical countries. a limited number of vaccines against arboviruses are available for use in humans; therefore, there is an urgent need to develop antiviral compounds. snake venoms are rich sources of bioactive compounds with potential for antiviral prospection. the major component of crotalus durissu ... | 2019 | 30809709 |
| photoreceptors morphology and genetics of the visual pigments of bothrops jararaca and crotalus durissus terrificus (serpentes, viperidae). | snakes inhabit a great variety of habitats, whose spectral quality of light may vary a lot and influence specific adaptations of their visual system. in this study, we investigated the genetics of the visual opsins and the morphology of retinal photoreceptors, of two nocturnal snakes from the viperidae family, bothrops jararaca and crotalus durissus terrificus, which inhabit preferentially the atlantic rain forest and the brazilian savannah, respectively. total rna was extracted from homogenized ... | 2019 | 30826356 |
| crotoxin promotes macrophage reprogramming towards an antiangiogenic phenotype. | crotoxin (ctx) is the primary toxin of south american rattlesnake crotalus durissus terrificus venom. ctx reduces tumour mass, and tumour cell proliferation and these effects seem to involve the formation of new vessels. angiogenesis has a key role in tumour growth and progression and is regulated by macrophage secretory activity. herein, the effect of ctx on macrophage secretory activity associated with angiogenesis was investigated in vitro. thymic endothelial cells (ec) were incubated in the ... | 2019 | 30862840 |
| secondary hemostasis studies of crude venom and isolated proteins from the snake crotalus durissus terrificus. | among the activities triggered by crotalus durissus terrificus snake venom, coagulation is intriguing and contradictory since the venom contains both coagulant and anticoagulant precursor proteins. this work describes the in vitro effects of crude venom and purified proteins from snake crotalus durissus terrificus as they affect coagulation factors of clotting pathways. coagulant and/or anticoagulant activities of crude venom, and purified proteins were all analyzed directly in human plasma. clo ... | 2019 | 30867125 |
| crotalus durissus terrificus (viperidae; crotalinae) envenomation: respiratory failure and treatment with antivipmyn tri® antivenom. | we report an envenomation to a professional herpetologist by a south american rattlesnake (crotalus durissus terrificus) that resulted in respiratory failure, and therapeutic improvement following antivenom administration. a 56-year-old male was bitten on the left wrist by a crotalus durissus terrificus (c. d. terrificus) while attempting to tube the snake for maintaining safe control while performing venom extraction. the patient was intubated due to rapidly ensuing respiratory failure and admi ... | 2019 | 30880190 |
| respiratory sinus arrhythmia is a major component of heart rate variability in undisturbed, remotely monitored rattlesnakes, crotalus durissus. | ecg recordings were obtained using an implanted telemetry device from the south american rattlesnake, crotalus durissus, held under stable conditions without restraining cables or interaction with researchers. mean heart rate (fh) recovered rapidly (<24 h) from anaesthesia and operative procedures. this preceded a more gradual development of heart rate variability (hrv), with instantaneous fh increasing during each lung ventilation cycle. atropine injection increased mean fh and abolished hrv. c ... | 2019 | 30967516 |
| molecular interactions between p-coumaric acid and snake venom toxins. | a large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. the interactions between the phenolic compound p-coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. the p-coumaric acid was able to inhibit 31% of the phospholipase activity induced by bothrops alternatus venom, 27% of the hemolytic activity induced by b. moojeni, 62.5% of the thrombolytic activ ... | 2019 | 31016790 |
| effects of crotoxin, a neurotoxin from crotalus durissus terrificus snake venom, on human endothelial cells. | vascular endothelium plays an important modulatory role due to the production of molecules that mediate vasomotricity, inflammation, and leukocyte adhesion and rolling. here we addressed whether crotoxin (25-200 μg/ml) - the main component of crotalus durissus terrificus snake venom - interferes with cell viability, apotosis/necrosis, and cell response to oxidative stress in human umbilical vein endothelial cells (huvec) in vitro. we also examined whether crotoxin alters the levels of interleuki ... | 2019 | 31071401 |
| crotoxin, a neurotoxin from crotalus durissus terrificus snake venom, as a potential tool against thrombosis development. | endothelium plays an important modulatory role due to the synthesis and secretion of molecules that act on hemostasis and fibrinolysis. as there are no literature data about the effect of crotoxin (ctx) - the main component of crotalus durissus terrificus snake venom - on human endothelial cells, the present study examined how ctx (25-200 μg/ml) affects the endothelial production of the hemostatic factors antithrombin iii, protein c, protein s, plasminogen activator inhibitor-1 (pai-1), tissue p ... | 2019 | 31071407 |
| insights into the structure, function and stability of bordonein-l, the first l-amino acid oxidase from crotalus durissus terrificus snake venom. | snake venom l-amino acid oxidases (svlaaos) are an interesting class of enzymes with important biological activities. their participation in key metabolic processes, including pathological disorders, suggest that svlaaos are potential lead compounds in drug discovery. however, their short-term stability defies their applications. this paper describes the stability studies together with functional and structural characterization of the laao bordonein-l. it has 498 amino acid residues, one n-glyco ... | 2019 | 31078582 |
| synthetic inhibitors of snake venom enzymes: thioesters derived from 2-sulfenyl ethylacetate. | snakebite envenomings are a global public health issue. the therapy based on the administration of animal-derived antivenoms has limited efficacy against the venom-induced local tissue damage, which often leads to permanent disability. therefore, there is a need to find inhibitors against toxins responsible for local damage. this work aimed to synthesize thioesters derived from 2-sulfenyl ethylacetate and to evaluate the inhibitory effects on two snake venom toxins. ethyl 2-((4-chlorobenzoyl)thi ... | 2019 | 31126073 |
| functional and biological insights of rcollinein-1, a recombinant serine protease from crotalus durissus collilineatus. | the prevalent class of snake venom serine proteases (svsp) in viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. however, thrombin-like serine proteases differ from thrombin by being unable to activate factor xiii, thus leading to the formation of loose clots and fibrinogen consumption. we report the functional and biological characterization of a recombinant thrombin-like serine protease from crotalus durissus co ... | 2019 | 31131001 |
| effects of the natural peptide crotamine from a south american rattlesnake on candida auris, an emergent multidrug antifungal resistant human pathogen. | invasive candida infections are an important growing medical concern and treatment options are limited to a few antifungal drug classes, with limited efficacies depending on the infecting organism. in this scenario, invasive infections caused by multiresistant candida auris are emerging in several places around the world as important healthcare-associated infections. as antimicrobial peptides (amps) exert their activities primarily through mechanisms involving membrane disruption, they have a lo ... | 2019 | 31141959 |
| radicicol enhances the regeneration of skeletal muscle injured by crotoxin via decrease of nf-kb activation. | this study evaluated cellular and molecular effects of radicicol, a heat shock protein (hsp) inducer, on the regeneration of skeletal muscle injured by crotoxin, the main toxin isolated from crotalus durissus terrificus venom. regenerating muscles treated with radicicol had decreased nf-kb activation. differentiating myoblasts treated with radicicol showed reduced number of nf-kb positive nuclei and increased fusion index. the results suggest that radicicol enhances regeneration of muscle by att ... | 2019 | 31173791 |
| jabuticaba (plinia jaboticaba) skin extracts as inhibitors of phospholipases a2 and proteases. | the phenolic extracts of jabuticaba skin flour (jsf) were characterized by hplc, and evaluated for their modulating action upon phospholipases a2 and proteases of snake venom, aiming at their possible use in the treatment of the various diseases associated with the action of venom toxins. two types of extracts were prepared from jsf: aqueous and methanolic. these extracts, evaluated at different ratios, (venom: extract, m/m), significantly inhibited the phospholipase activity induced by the veno ... | 2019 | 31269103 |
| evaluation of the inhibitory potential of casuarictin, an ellagitannin isolated from white mangrove (laguncularia racemosa) leaves, on snake venom secretory phospholipase a2. | ellagitannins constitute the largest group of hydrolyzable tannins of plants, and, from this group, casuarictin (casu) was identified in some plant species. however, to our knowledge, no investigation of secretory phospholipase a2 (spla2) inhibition by casu has been performed yet. casuarictin was isolated by chromatography n-butanol (n-buoh) partition of laguncularia racemosa leaves. the pharmacological and biological effects of casu were evaluated on isolated spla2 from the rattlesnake (crotalu ... | 2019 | 31288445 |
| thermal regime effects on the resting metabolic rate of rattlesnakes depend on temperature range. | while ectothermic organisms often experience considerable circadian variation in body temperature under natural conditions, the study of the effects of temperature on metabolic rates are traditionally based on subjecting animals to constant temperature regimes. whether data resulting from constant-temperature experiments accurately predicts temperature effects under more natural fluctuating temperature regimes remains uncertain. to address such possibility, we measured the resting metabolic rate ... | 2019 | 31331520 |
| venoms and isolated toxins from snakes of medical impact in the northeast argentina: state of the art. potential pharmacological applications. | among the ophidians that inhabit the northeast of argentina, the genus bothrops such as b. alternatus and b. diporus species (also known as yararás) and crotalus durisus terrificus (named cascabel), represent the most studied snake venom for more than thirty years. these two genera of venomous snakes account for the majority of poisonous snake envenomations and therefore, constitute a medical emergency in this region. this review presents a broad description of the compiled knowledge about venom ... | 2019 | 31345151 |
| clinical, laboratory, and therapeutic aspects of crotalus durissus (south american rattlesnake) victims: a literature review. | snakebite envenoming is a neglected public health issue in many tropical and subtropical countries. to diagnosis and treat snakebites may be challenging to health care personnel since sufficient information has not been yet provided. this review presents the clinical, therapeutic, and laboratory aspects of crotalus durissus (south american rattlesnakes) victims. the clinical setting may show local effects such as little or no pain, mild edema, and recurrent erythema. in contrast, the systemic ef ... | 2019 | 31467868 |
| local and systemic effects caused by crotalus durissus terrificus, crotalus durissus collilineatus, and crotalus durissus cascavella snake venoms in swiss mice. | crotalus envenomations cause serious complications and can be fatal without appropriate treatment. venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. we comparatively evaluated the local and systemic effects caused by crotalus durissus terrificus (cdt), c.d. collilineatus (cdcolli), and c.d. cascavella (cdcasc) envenomation. | 2019 | 31508780 |
| exposure to fluctuating temperatures leads to reduced immunity and to stress response in rattlesnakes. | ectothermic organisms often experience considerable variation in their body temperature throughout the circadian cycle. however, studies focusing on the measurement of physiological traits are usually performed under constant temperature regimes. this mismatch between thermal exposure in the field and experimental conditions could act as a stressor agent, as physiological functions are strongly influenced by temperature. herein, we asked the question whether constant thermal regimes would cause ... | 2019 | 31672725 |
| no mediates the effect of the synthetic natriuretic peptide npcdc on kidney and aorta in nephrectomised rats. | npcdc is a synthetic natriuretic peptide that was originally derived from another peptide, the np2_casca, isolated from crotalus durissus cascavella venom. these molecules share 70% structural homology with natriuretic peptides obtained from different species, including humans. np2_casca induces vasorelaxation and increases nitric oxide levels independently of natriuretic peptide receptors a and b. this study aimed to investigate whether npcdc-induced hypotension in control rats and rats with a ... | 2020 | 31734277 |
| crotoxin conjugated to sba-15 nanostructured mesoporous silica induces long-last analgesic effect in the neuropathic pain model in mice. | neuropathic pain is a disease caused by structural and functional plasticity in central and peripheral sensory pathways that produce alterations in nociceptive processing. currently, pharmacological treatment for this condition remains a challenge. crotoxin (ctx), the main neurotoxin of crotalus durissus terrificus rattlesnake venom, has well described prolonged anti-inflammatory and antinociceptive activities. in spite of its potential benefits, the toxicity of ctx remains a limiting factor for ... | 2019 | 31757011 |
| crotalus durissus ruruima snake venom and a phospholipase a2 isolated from this venom elicit macrophages to form lipid droplets and synthesize inflammatory lipid mediators. | viper snake crotalus durissus ruruima (cdr) is a subspecies found in northern area of brazil. among the snakes of crotalus genus subspecies, the venom of cdr presents highest level of crotoxin, which is the major component of crotalus snake venoms, formed by two subunits (crotapotin and a phospholipase a2 named cbr) and presents potent neurotoxic activity. curiously, the venom of c. d. ruruima (cdrv) is better neutralized by antibothropic than by anticrotalic serum, strongly suggesting that this ... | 2019 | 31781674 |
| unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review. | fibrin biopolymers, previously referred as "fibrin glue" or "fibrin sealants", are natural biomaterials with diverse applications on health. they have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. in the 1990s, to overcome th ... | 2019 | 31839802 |
| crotoxin down-modulates pro-inflammatory cells and alleviates pain on the mog35-55-induced experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. | multiple sclerosis (ms) is a central nervous system inflammatory demyelinating disease that has as primary symptoms losses of sensory and motor functions, including chronic pain. to date, however, few studies have investigated the mechanisms of chronic pain in animal models of ms since locomotor impairments render difficult its evaluation. it was previously demonstrated that in the mog35-55-induced eae, an animal model of ms, the hypernociception appears before the onset of motor disability, all ... | 2020 | 31843645 |
| crotamine and crotalicidin, membrane active peptides from crotalus durissus terrificus rattlesnake venom, and their structurally-minimized fragments for applications in medicine and biotechnology. | a global public health crisis has emerged with the extensive dissemination of multidrug-resistant microorganisms. antimicrobial peptides (amps) from plants and animals have represented promising tools to counteract those resistant pathogens due to their multiple pharmacological properties such as antimicrobial, anticancer, immunomodulatory and cell-penetrating activities. in this review, we will focus on crotamine and crotalicidin, which are two interesting examples of membrane active peptides d ... | 2020 | 31857106 |
| defensive behaviors and brain regional activation changes in rats confronting a snake. | in the present study, we examined behavioral and brain regional activation changes of rats). to a nonmammalian predator, a wild rattler snake (crotalus durissus terrificus). accordingly, during snake threat, rat subjects showed a striking and highly significant behavioral response of freezing, stretch attend, and, especially, spatial avoidance of this threat. the brain regional activation patterns for these rats were in broad outline similar to those of rats encountering other predator threats, ... | 2020 | 31917239 |
| bioaffinity fishing procedure using secretory phospholipase a2 for screening for bioactive components: modulation of pharmacological effect induced by spla2 from crotalus durissus terrificus by hispidulin from moquiniastrum floribundum. | bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. however, some proteins, such as snake secretory phospholipase a2 (spla2), have never been investigated using this approach. the purpose of this study was to evaluate the use of ... | 2020 | 31936688 |
| phylogenetic analysis of hepatozoon spp. (apicomplexa: hepatozoidae) infecting philodryas patagoniensis (serpentes: dipsadidae) in uruguay. | the purpose of this study was to perform a phylogenetic analysis of hepatozoon spp. infecting philodryas patagoniensis in uruguay. twenty-five road-killed specimens of p. patagoniensis from ten departments were obtained. samples of blood and/or heart tissue were taken. polymerase chain reaction (pcr) assay was carried out amplifying a specific target region of the 18s rrna gene of hepatozoon spp. eighteen out of twenty-five samples were positive to hepatozoon spp., which gave an overall prevalen ... | 2020 | 31938888 |
| effect of isolated proteins from crotalus durissus terrificus venom on leishmania (leishmania) amazonensis-infected macrophages. | cutaneous and mucocutaneous leishmaniasis are parasitic diseases characterized by skin manifestations. in brazil, leishmania (leishmania) amazonensis is one of the etiological agents of cutaneous leishmaniasis. the therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, as they are often highly toxic, poorly tolerated and of variable effectiveness. this study aimed to evaluate in vitro the leishmanicidal activity of toxins isol ... | 2020 | 31994997 |
| engineered protein containing crotoxin epitopes induces neutralizing antibodies in immunized rabbits. | crotoxin (ctx) is the main lethal component of crotalus durissus terrificus venom. it is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (ca), named crotapotin, and the basic subunit (cb), with phospholipase a2 (pla2) activity. employing the spot synthesis technique, we determined two epitopes located in the c-terminal of each ctx subunit. in addition, 3 other epitopes were mapped in different regions of ctx using subcutaneous spot implan ... | 2020 | 32023500 |
| evaluation of systemic inflammatory response and lung injury induced by crotalus durissus cascavella venom. | this study investigated the systemic inflammatory response and mechanism of pulmonary lesions induced by crotalus durissus cascavella venom in murine in the state of bahia. in order to investigate t helper th1, th2 and th17 lymphocyte profiles, we measured interleukin (il) -2, il-4, il-6, il-10, il-17, tumor necrosis factor (tnf) and interferon gamma (ifn-γ) levels in the peritoneal fluid and macerated lungs of mice and histopathological alterations at the specific time windows of 1h, 3h, 6h, 12 ... | 2020 | 32084665 |
| varespladib (ly315920) and methyl varespladib (ly333013) abrogate or delay lethality induced by presynaptically acting neurotoxic snake venoms. | the phospholipase a2 (pla2) inhibitor varespladib (ly315920) and its orally bioavailable prodrug, methyl-varespladib (ly333013) inhibit pla2 activity of a wide variety of snake venoms. in this study, the ability of these two forms of varespladib to halt or delay lethality of potent neurotoxic snake venoms was tested in a mouse model. the venoms of notechis scutatus, crotalus durissus terrificus, bungarus multicinctus, and oxyuranus scutellatus, all of which have potent presynaptically acting neu ... | 2020 | 32093386 |
| experimental model for removal of snake venom via hemoperfusion in rats. | to examine the efficiency of hemoperfusion in removing south american rattlesnake (crotalus durissus terrificus) venom from rats compared with neutralization by antivenom. | 2020 | 32112523 |
| beyond hemostasis: a snake venom serine protease with potassium channel blocking and potential antitumor activities. | snake venom serine proteases (svsps) are complex and multifunctional enzymes, acting primarily on hemostasis. in this work, we report the hitherto unknown inhibitory effect of a svsp, named collinein-1, isolated from the venom of crotalus durissus collilineatus, on a cancer-relevant voltage-gated potassium channel (heag1). among 12 voltage-gated ion channels tested, collinein-1 selectively inhibited heag1 currents, with a mechanism independent of its enzymatic activity. corroboratively, we demon ... | 2020 | 32161292 |
| crotalus neutralising factor and its role in human leukocyte modulation. | crotalus neutralising factor (cnf) is an endogenous γ-type phospholipase a2 (pla2) inhibitor that inhibits the toxic action of crotoxin, a neurotoxin present in crotalus durissus terrificus venom. however, its effects on the activation and modulation of immune cells, which play a major role in the development of inflammation, is not known. the objective of the present study was to assess the effects of cnf on human leukocyte modulation in vitro by analysing the following parameters: cell viabili ... | 2020 | 32183984 |
| sulfur compounds as inhibitors of enzymatic activity of a snake venom phospholipase a2: benzyl 4-nitrobenzenecarbodithioate as a case of study. | snakebite is a neglected disease with a high impact in tropical and subtropical countries. therapy based on antivenom has limited efficacy in local tissue damage caused by venoms. phospholipases a2 (pla2) are enzymes that abundantly occur in snake venoms and induce several systemic and local effects. furthermore, sulfur compounds such as thioesters have an inhibitory capacity against a snake venom pla2. hence, the objective of this work was to obtain a carbodithioate from a thioester with known ... | 2020 | 32197309 |
| accessing cryptic diversity in neotropical rattlesnakes (serpentes: viperidae: crotalus) with the description of two new species. | members of the crotalus durissus species complex are widely distributed from mexico to argentina in areas with mainly seasonally dry tropical deciduous forest. although four species (c. culminatus, c. durissus, c. simus and c. tzabcan) are currently recognized, species limits remain to be tested. previous genetic studies suggest that c. durissus and c. simus may be paraphyletic and that at least one cryptic species may be present. we analyzed 2596 bp of dna sequence data from three mitochondrial ... | 2020 | 32229836 |
| identification and characterization of the first endogenous phospholipase a2 inhibitor from a non-venomous tropical snake, boa constrictor (serpentes: boidae). | endogenous phospholipase a2 inhibitors from snake blood (sbplis) have been isolated from several species around the world, with the primary function of self-protection against the action of toxic phospholipases a2. in american snakes, sbplis were solely described in pit vipers, in which the natural protection role is justified. in this study, we described a sbpli in boa constrictor (popularly known as jiboia), a non-venomous snake species from america. | 2020 | 32231698 |
| the impact of rattlesnake venom on mice cerebellum proteomics points to synaptic inhibition and tissue damage. | snake envenomation is responsible for more than 130,000 deaths worldwide. in brazil, the crotalus rattlesnake is responsible for the second largest number of accidental snake bites in the country. although there are many descriptions of the clinical and biochemical effects of crotalus envenoming, there are few works describing the molecular events in the central nervous system of an organism due to envenomation. in this study, we analyzed the proteomic effect of crotalus durissus terrificus snak ... | 2020 | 32272218 |
| toxinological characterization of venom from leptodeira annulata (banded cat-eyed snake; dipsadidae, imantodini). | we investigated the histology of duvernoy's venom gland and the biochemical and biological activities of leptodeira annulata snake venom. the venom gland had a lobular organization, with secretory tubules formed by serous epithelial cells surrounding each lobular duct. the latter drained into a common lobular duct and subsequently into a central cistern. in contrast, the supralabial gland was mucous in nature. sds-page revealed a profile of venom components that differed from pitviper (bothrops ... | 2020 | 32302625 |
| use of a phosphopeptide as a ligand to purify phospholipase a2 from the venom of crotalus durisuss terrificus by affinity chromatography. | the venom of crotalus durissus terrificus (cdt) is a source of a wide variety of toxins, some of them with interesting pharmacological applications. of these toxins, the phospholipase a2 (pla2) subunit of crotoxin (ctx) has been studied for its potential as an antiviral and antibacterial agent. peptides have proven useful ligands for the purification of numerous molecules, including antibodies, toxins, enzymes and other proteins. here, we sought to use a phosphopeptide (p-lys) as a ligand for pl ... | 2020 | 32361466 |