Publications

settling down: the genome of serratia symbiotica from the aphid cinara tujafilina zooms in on the process of accommodation to a cooperative intracellular life.	particularly interesting cases of mutualistic endosymbioses come from the establishment of co-obligate associations of more than one species of endosymbiotic bacteria. throughout symbiotic accommodation from a free-living bacterium, passing through a facultative stage and ending as an obligate intracellular one, the symbiont experiences massive genomic losses and phenotypic adjustments. here, we scrutinized the changes in the coevolution of serratia symbiotica and buchnera aphidicola endosymbion ...	2014	24951564
identification and comparative analysis of g protein-coupled receptors in pediculus humanus humanus.	the body louse has the smallest genome size among the known genome-sequenced insects. here, 81 gpcrs were identified in pediculus humanus humanus, 56, 14, 6 and 5 gpcrs for family-a, -b, -c and -d, respectively. these gpcrs constitute the comparable repertoire of gpcrs with other insects. moreover, it contains a more complete set of neuropeptide and protein hormone receptors not even than acyrthosiphon pisum but also drosophila melanogaster, for example, sulfakinin, corazonin, trissin and pthrl ...	2014	24952173
genomic regions repeatedly involved in divergence among plant-specialized pea aphid biotypes.	understanding the genetic bases of biological diversification is a long-standing goal in evolutionary biology. here, we investigate whether replicated cases of adaptive divergence involve the same genomic regions in the pea aphid, acyrthosiphon pisum, a large complex of genetically differentiated biotypes, each specialized on different species of legumes. a previous study identified genomic regions putatively involved in host-plant adaptation and/or reproductive isolation by performing a hierarc ...	2014	24953130
widespread host-dependent hybrid unfitness in the pea aphid species complex.	linking adaptive divergence to hybrid unfitness is necessary to understand the ecological factors contributing to reproductive isolation and speciation. to date, this link has been demonstrated in few model systems, most of which encompass ecotypes that occupy relatively early stages in the speciation process. here we extend these studies by assessing how host-plant adaptation conditions hybrid fitness in the pea aphid, acyrthosiphon pisum. we made crosses between and within five pea aphid bioty ...	2014	24957707
molecular characterization of the aphis gossypii olfactory receptor gene families.	the cotton aphid, aphis gossypii glover, is a polyphagous pest that inflicts great damage to cotton yields worldwide. antennal olfaction, which is extremely important for insect survival, mediates key behaviors such as host preference, mate choice, and oviposition site selection. in insects, odor detection is mediated by odorant receptors (ors) and ionotropic receptors (irs), which ensure the specificity of the olfactory sensory neuron responses. in this study, our aim is to identify chemosensor ...	2014	24971460
factors limiting the spread of the protective symbiont hamiltonella defensa in aphis craccivora aphids.	many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. the cowpea aphid, aphis craccivora, is a polyphagous pest that harbors hamiltonella defensa, which defends against parasitic wasps. despite this protective benefit, this symbiont occurs only at intermediate frequencies in field populations. to identify factors constraining h. defensa invasion in ap. craccivora, we estimated symbiont transmission rates, per ...	2014	25015890
matching the supply of bacterial nutrients to the nutritional demand of the animal host.	various animals derive nutrients from symbiotic microorganisms with much-reduced genomes, but it is unknown whether, and how, the supply of these nutrients is regulated. here, we demonstrate that the production of essential amino acids (eaas) by the bacterium buchnera aphidicola in the pea aphid acyrthosiphon pisum is elevated when aphids are reared on diets from which that eaa are omitted, demonstrating that buchnera scale eaa production to host demand. quantitative proteomics of bacteriocytes ...	2014	25080346
genome-wide annotation and functional identification of aphid glut-like sugar transporters.	phloem feeding insects, such as aphids, feed almost continuously on plant phloem sap, a liquid diet that contains high concentrations of sucrose (a disaccharide comprising of glucose and fructose). to access the available carbon, aphids hydrolyze sucrose in the gut lumen and transport its constituent monosaccharides, glucose and fructose. although sugar transport plays a critical role in aphid nutrition, the molecular basis of sugar transport in aphids, and more generally across all insects, rem ...	2014	25091229
the stimuli evoking the aerial-righting posture of falling pea aphids.	some wingless insects possess aerial righting reflexes, suggesting that adaptation for controlling body orientation while falling through air could have preceded flight. when threatened by natural enemies, wingless pea aphids (acyrthosiphon pisum) may drop off their host plant and assume a stereotypic posture that rotates them in midair to land on their feet. the sensory information triggering aphids to assume this posture has so far been unknown. we subjected aphids to a series of tests, isolat ...	2014	25104755
analysis of new aphid lethal paralysis virus (alpv) isolates suggests evolution of two alpv species.	aphid lethal paralysis virus (alpv; family dicistroviridae) was first isolated from the bird cherry-oat aphid, rhopalosiphum padi. alpv-like virus sequences have been reported from many insects and insect predators. we identified a new isolate of alpv (alpv-ap) from the pea aphid, acyrthosiphon pisum, and a new isolate (alpv-dvv) from western corn rootworm, diabrotica virgifera virgifera. alpv-ap has an ssrna genome of 9940 nt. based on phylogenetic analysis, alpv-ap was closely related to alpv- ...	2014	25170050
development times and age-specific life table parameters of the native lady beetle species coccinella novemnotata (coleoptera: coccinellidae) and its invasive congener coccinella septempunctata (coleoptera: coccinellidae).	to determine if differences in life history parameters contribute to native species exclusion, immature development times, larval survivorship, reproductive life history parameters, and age-specific life tables were determined for two populations (eastern united states and western united states) of ninespotted lady beetles (coccinella novemnotata herbst) and one population of sevenspotted lady beetles (coccinella septempunctata l.). developing larvae were provided an ad libitum diet of pea aphid ...	2014	25182621
diversification of mif immune regulators in aphids: link with agonistic and antagonistic interactions.	the widespread use of genome sequencing provided evidences for the high degree of conservation in innate immunity signalling pathways across animal phyla. however, the functioning and evolutionary history of immune-related genes remains unknown for most invertebrate species. a striking observation coming from the analysis of the pea aphid acyrthosiphon pisum genome is the absence of important conserved genes known to be involved in the antimicrobial responses of other insects. this reduction in ...	2014	25193628
anticipatory and reactive crouching of pea aphids in response to environmental perturbations.	animals use different strategies to deal with changing environmental conditions. while standing and feeding on their host plant, aphids (hemiptera: aphididae) may be exposed to detrimental environmental perturbations, such as strong winds. if aphids are forcibly blown off the plant and spend time on the ground, they will face additional dangers by both ground-dwelling predators and detrimental soil temperature. it is therefore adaptive for aphids to behave in a way that lowers the risk of being ...	2014	25198197
increasing phosphorus supply is not the mechanism by which arbuscular mycorrhiza increase attractiveness of bean (vicia faba) to aphids.	arbuscular mycorrhizal (am) fungi, important plant mutualists, provide plants with nutrients such as phosphorus (p) in return for carbon. am fungi also enhance the attractiveness of plants to aphids via effects on emissions of plant volatiles used in aphid host location. we tested whether increased p uptake by plants is the mechanism through which am fungi alter the volatile profile of plants and aphid behavioural responses by manipulating the availability of p and am fungi to broad beans (vicia ...	2014	25200735
evolution of gonadotropin-inhibitory hormone receptor and its ligand.	gonadotropin-inhibitory hormone (gnih) is a neuropeptide inhibitor of gonadotropin secretion, which was first identified in the japanese quail hypothalamus. gnih peptides share a c-terminal lpxrfamide (x=l or q) motif in most vertebrates. the receptor for gnih (gnihr) is the seven-transmembrane g protein-coupled receptor 147 (gpr147) that inhibits camp production. gpr147 is also named neuropeptide ff (npff) receptor 1 (npffr1), because it also binds npff that has a c-terminal pqrfamide motif. to ...	2014	25220854
dynamics of copy number variation in host races of the pea aphid.	copy number variation (cnv) makes a major contribution to overall genetic variation and is suspected to play an important role in adaptation. however, aside from a few model species, the extent of cnv in natural populations has seldom been investigated. here, we report on cnv in the pea aphid acyrthosiphon pisum, a powerful system for studying the genetic architecture of host-plant adaptation and speciation thanks to multiple host races forming a continuum of genetic divergence. recent studies h ...	2015	25234705
stem nematode counteracts plant resistance of aphids in alfalfa, medicago sativa.	plants are exploited by a diverse community of insect herbivores and phytopathogens that interact indirectly through plant-mediated interactions. generally, plants are thought to respond to insects and pathogens through different defensive signaling pathways. as plants are selected for resistance to one phytophagous organism type (insect vs. pathogen) in managed systems, it is not clear how this selection may affect community interactions. this study examined the effect of nematode-resistant var ...	2014	25261892
whole-genome re-sequencing of non-model organisms: lessons from unmapped reads.	unmapped reads are often discarded from the analysis of whole-genome re-sequencing, but new biological information and insights can be uncovered through their analysis. in this paper, we investigate unmapped reads from the re-sequencing data of 33 pea aphid genomes from individuals specialized on different host plants. the unmapped reads for each individual were retrieved following mapping to the acyrthosiphon pisum reference genome and its mitochondrial and symbiont genomes. these sets of unmap ...	2014	25269379
faba bean forisomes can function in defence against generalist aphids.	phloem sieve elements have shut-off mechanisms that prevent loss of nutrient-rich phloem sap when the phloem is damaged. some phloem proteins such as the proteins that form forisomes in legume sieve elements are one such mechanism and in response to damage, they instantly form occlusions that stop the flow of sap. it has long been hypothesized that one function of phloem proteins is defence against phloem sap-feeding insects such as aphids. this study provides the first experimental evidence tha ...	2015	25311512
plant genetic variation mediates an indirect ecological effect between belowground earthworms and aboveground aphids.	interactions between aboveground and belowground terrestrial communities are often mediated by plants, with soil organisms interacting via the roots and aboveground organisms via the shoots and leaves. many studies now show that plant genetics can drive changes in the structure of both above and belowground communities; however, the role of plant genetic variation in mediating aboveground-belowground interactions is still unclear. we used an earthworm-plant-aphid model system with two aphid spec ...	2014	25331082
pea aphid biotype performance on diverse medicago host genotypes indicates highly specific virulence and resistance functions.	aphid-plant interactions depend on genotypes of both organisms, which determine the two-way molecular exchange that leads to compatible or incompatible outcomes. the underlying genes are mostly unknown, making it difficult to predict likelihood of aphid success or host resistance, and hampering crop genetic improvement. here we screened eight pea aphid clonal genotypes collected from diverse legume hosts, on a species-wide panel of medicago truncatula (mt) genotypes. aphid virulence was measured ...	2014	25375216
do defensive chemicals facilitate intraguild predation and influence invasion success in ladybird beetles?	egg predation and cannibalism are believed to be common phenomena among many species of aphidophagous predatory ladybird beetles despite the presence of alkaloid based defensive chemicals in all life stages. we identified defensive chemicals from eggs of three congeneric species, one introduced into north america (coccinella septempunctata l.), and two native (c. transversoguttata richardsoni brown, and c. novemnotata herbst), and examined the effects of ingested defensive chemicals on first ins ...	2014	25380992
an experimental test of whether the defensive phenotype of an aphid facultative symbiont can respond to selection within a host lineage.	an experiment was conducted to test whether parasitoid resistance within a single clonal line of pea aphid (acyrthosiphon pisum) might increase after exposure to the parasitoid wasp aphidius ervi. any change in resistance was expected to occur through an increase in the density of protective symbiotic bacteria rather than genetic change within the aphid or the bacterial symbiont. six aphid lineages were exposed to high parasitoid attack rates over nine generations, each line being propagated fro ...	2014	25396733
amino acid-mediated impacts of elevated carbon dioxide and simulated root herbivory on aphids are neutralized by increased air temperatures.	changes in host plant quality, including foliar amino acid concentrations, resulting from global climate change and attack from multiple herbivores, have the potential to modify the pest status of insect herbivores. this study investigated how mechanically simulated root herbivory of lucerne (medicago sativa) before and after aphid infestation affected the pea aphid (acyrthosiphon pisum) under elevated temperature (et) and carbon dioxide concentrations (eco2). et increased plant height and bioma ...	2015	25403916
adenine methylation may contribute to endosymbiont selection in a clonal aphid population.	the pea aphid acyrthosiphon pisum has two modes of reproduction: parthenogenetic during the spring and summer and sexual in autumn. this ability to alternate between reproductive modes and the emergence of clonal populations under favorable conditions make this organism an interesting model for genetic and epigenetic studies. the pea aphid hosts different types of endosymbiotic bacteria within bacteriocytes which help the aphids survive and adapt to new environmental conditions and habitats. the ...	2014	25406741
selection of reference genes for expression analysis using quantitative real-time pcr in the pea aphid, acyrthosiphon pisum (harris) (hemiptera, aphidiae).	to facilitate gene expression study and obtain accurate qrt-pcr analysis, normalization relative to stable expressed housekeeping genes is required. in this study, expression profiles of 11 candidate reference genes, including actin (actin), elongation factor 1 α (ef1a), tata-box-binding protein (tata), ribosomal protein l12 (rpl12), β-tubulin (tubulin), nadh dehydrogenase (nadh), vacuolar-type h+-atpase (v-atpase), succinate dehydrogenase b (sdhb), 28s ribosomal rna (28s), 16s ribosomal rna (16 ...	2014	25423476
comparative transcriptomic analyses revealed divergences of two agriculturally important aphid species.	grain aphid (sitobion avenae f) and pea aphid (acyrthosiphon pisum) are two agriculturally important pest species, which cause significant yield losses to crop plants each year by inflicting damage both through the direct effects of feeding and by vectoring debilitating plant viruses. although a close phylogenetic relationship between grain aphid and pea aphid was proposed, the biological variations between these two aphid species are obvious. while the host ranges of grain aphid is restricted t ...	2014	25424897
expression of stress-related genes in the parthenogenetic forms of the pea aphid, acyrthosiphon pisum.	aphids are an economically important group of insects that have an intricate life cycle with seasonal polyphenism. this study aimed to explore the physiological background of aphid migration from unfavorable nutritional conditions to a new, intact host plant. specifically, the relative expression of stress/metabolism-related genes and changes in metabolic reserves were determined for the winged and wingless forms of female pea aphids, acyrthosiphon pisum, under two different nutritional conditio ...	2015	25461486
genetic control of contagious asexuality in the pea aphid.	although evolutionary transitions from sexual to asexual reproduction are frequent in eukaryotes, the genetic bases of such shifts toward asexuality remain largely unknown. we addressed this issue in an aphid species where both sexual and obligate asexual lineages coexist in natural populations. these sexual and asexual lineages may occasionally interbreed because some asexual lineages maintain a residual production of males potentially able to mate with the females produced by sexual lineages. ...	2014	25473828
gene expression analysis of parthenogenetic embryonic development of the pea aphid, acyrthosiphon pisum, suggests that aphid parthenogenesis evolved from meiotic oogenesis.	aphids exhibit a form of phenotypic plasticity, called polyphenism, in which genetically identical females reproduce sexually during one part of the life cycle and asexually (via parthenogenesis) during the remainder of the life cycle. the molecular basis for aphid parthenogenesis is unknown. cytological observations of aphid parthenogenesis suggest that asexual oogenesis evolved either through a modification of meiosis or from a mitotic process. as a test of these alternatives, we assessed the ...	2014	25501006
cryptic virulence and avirulence alleles revealed by controlled sexual recombination in pea aphids.	although aphids are worldwide crop pests, little is known about aphid effector genes underlying virulence and avirulence. here we show that controlling the genetics of both aphid and host can reveal novel recombinant genotypes with previously undetected allelic variation in both virulence and avirulence functions. clonal f1 progeny populations were derived from reciprocal crosses and self-matings between two parental genotypes of pea aphid (acyrthosiphon pisum) differing in virulence on a medica ...	2015	25519896
a substrate ambiguous enzyme facilitates genome reduction in an intracellular symbiont.	genome evolution in intracellular microbial symbionts is characterized by gene loss, generating some of the smallest and most gene-poor genomes known. as a result of gene loss these genomes commonly contain metabolic pathways that are fragmented relative to their free-living relatives. the evolutionary retention of fragmented metabolic pathways in the gene-poor genomes of endosymbionts suggests that they are functional. however, it is not always clear how they maintain functionality. to date, th ...	2014	25527092
the structural sheath protein of aphids is required for phloem feeding.	aphids produce two types of saliva that mediate their interactions with plants. watery saliva is secreted during cell penetration and ingestion, whereas gel saliva is secreted during stylet movement through the apoplast where it forms a sheath around the stylet to facilitate penetration and seal puncture sites on cell membranes. in order to study the function of the sheath when aphids interact with plants, we used rna interference (rnai) to silence the aphid structural sheath protein (shp) in th ...	2015	25527379
aphid salivary proteases are capable of degrading sieve-tube proteins.	sieve tubes serve as transport conduits for photo-assimilates and other resources in angiosperms and are profitable targets for piercing-sucking insects such as aphids. sieve-tube sap also contains significant amounts of proteins with diverse functions, for example in signalling, metabolism, and defence. the identification of salivary proteases in acyrthosiphon pisum led to the hypothesis that aphids might be able to digest these proteins and by doing so suppress plant defence and access additio ...	2015	25540441
detection and decay rates of prey and prey symbionts in the gut of a predator through metagenomics.	dna methods are useful to identify ingested prey items from the gut of predators, but reliable detection is hampered by low amounts of degraded dna. pcr-based methods can retrieve minute amounts of starting material but suffer from amplification biases and cross-reactions with the predator and related species genomes. here, we use pcr-free direct shotgun sequencing of total dna isolated from the gut of the harlequin ladybird harmonia axyridis at five time points after feeding on a single pea aph ...	2015	25545417
experimental replacement of an obligate insect symbiont.	symbiosis, the close association of unrelated organisms, has been pivotal in biological diversification. in the obligate symbioses found in many insect hosts, organisms that were once independent are permanently and intimately associated, resulting in expanded ecological capabilities. the primary model for this kind of symbiosis is the association between the bacterium buchnera and the pea aphid (acyrthosiphon pisum). a longstanding obstacle to efforts to illuminate genetic changes underlying ob ...	2015	25561531
plant stomatal closure improves aphid feeding under elevated co2.	stomata help plants regulate co2 absorption and water vapor release in response to various environmental changes, and plants decrease their stomatal apertures and enhance their water status under elevated co2 . although the bottom-up effect of elevated co2 on insect performance has been extensively studied, few reports have considered how insect fitness is altered by elevated co2 -induced changes in host plant water status. we tested the hypothesis that aphids induce stomatal closure and increas ...	2015	25581722
isolation and identification of culturable bacteria from honeydew of whitefly, bemisia tabaci (g.) (hemiptera: aleyrodidae).	bemisia tabaci (g.) is an important pest and a vector of gemini viruses infecting plants. during the process of feeding b. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. recent report proved that volatile emitted by the honeydew associated bacteria of aphid, acyrthosiphon pisum harris was involved in natural enemy calling. thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest ...	2014	25606395
transcriptome analysis of sexually dimorphic chinese white wax scale insects reveals key differences in developmental programs and transcription factor expression.	the chinese white wax scale insect, ericerus pela, represents one of the most dramatic examples of sexual dimorphism in any insect species. in this study, we showed that although e. pela males display complete metamorphosis similar to holometabolous insects, the species forms the sister group to acyrthosiphon pisum and cluster with hemimetabolous insects. the gene expression profile and gene ontology (go) analyses revealed that the two sexes engaged in distinct developmental programs. in particu ...	2015	25634031
a ruthenium(ii) complex as turn-on cu(ii) luminescent sensor based on oxidative cyclization mechanism and its application in vivo.	copper ions play a vital role in a variety of fundamental physiological processes not only in human beings and plants, but also for extensive insects and microorganisms. in this paper, a novel water-soluble ruthenium(ii) complex as a turn-on copper(ii) ions luminescent sensor based on o-(phenylazo)aniline was designed and synthesized. the azo group would undergo a specific oxidative cyclization reaction with copper(ii) ions and turn into high luminescent benzotriazole, triggering significant lum ...	2015	25640000
armet is an effector protein mediating aphid-plant interactions.	aphid saliva is predicted to contain proteins that modulate plant defenses and facilitate feeding. armet is a well-characterized bifunctional protein in mammalian systems. here we report a new role of armet, namely as an effector protein in the pea aphid, acyrthosiphon pisum. pea aphid armet's physical and chemical properties and its intracellular role are comparable to those reported for mammalian armets. uniquely, we detected armet in aphid watery saliva and in the phloem sap of fava beans fed ...	2015	25678626
patterns, causes and consequences of defensive microbiome dynamics across multiple scales.	the microbiome can significantly impact host phenotypes and serve as an additional source of heritable genetic variation. while patterns across eukaryotes are consistent with a role for symbiotic microbes in host macroevolution, few studies have examined symbiont-driven host evolution or the ecological implications of a dynamic microbiome across temporal, spatial or ecological scales. the pea aphid, acyrthosiphon pisum, and its eight heritable bacterial endosymbionts have served as a model for s ...	2015	25683348
two host clades, two bacterial arsenals: evolution through gene losses in facultative endosymbionts.	bacterial endosymbiosis is an important evolutionary process in insects, which can harbor both obligate and facultative symbionts. the evolution of these symbionts is driven by evolutionary convergence, and they exhibit among the tiniest genomes in prokaryotes. the large host spectrum of facultative symbionts and the high diversity of strategies they use to infect new hosts probably impact the evolution of their genome and explain why they undergo less severe genomic erosion than obligate symbio ...	2015	25714744
molecular features and toxicological properties of four common pesticides, acetamiprid, deltamethrin, chlorpyriphos and fipronil.	structural features and selected physicochemical properties of four common pesticides: acetamiprid (neonicotinoid), chlorpyriphos (organophosphate insecticide), deltamethrin (pyrethroid) and fipronil (phenylpyrazole) have been investigated by density functional theory quantum chemical calculations. the high flexible character of these insecticides is revealed by the numerous conformers obtained, located within a 20kjmol(-1) range in the gas phase. in line with this trend, a redistribution of the ...	2015	25716006
carbon dioxide receptor genes in cotton bollworm helicoverpa armigera.	carbon dioxide (co2) is important in insect ecology, eliciting a range of behaviours across different species. interestingly, the numbers of co2 gustatory receptors (grs) vary among insect species. in the model organism drosophila melanogaster, two grs (dmelgr21a and dmelgr63a) have been shown to detect co2. in the butterfly, moth, beetle and mosquito species studied so far, three co2 gr genes have been identified, while in tsetse flies, four co2 gr genes have been identified. in other species i ...	2015	25724420
new cyt-like δ-endotoxins from dickeya dadantii: structure and aphicidal activity.	in the track of new biopesticides, four genes namely cyta, cytb, cytc and cytd encoding proteins homologous to bacillus thuringiensis (bt) cyt toxins have been identified in the plant pathogenic bacteria dickeya dadantii genome. here we show that three cyt-like δ-endotoxins from d. dadantii (cyta, cytb and cytc) are toxic to the pathogen of the pea aphid acyrthosiphon pisum in terms of both mortality and growth rate. the phylogenetic analysis of the comprehensive set of cyt toxins available in g ...	2015	25740111
angiotensin-converting enzymes modulate aphid-plant interactions.	angiotensin-converting enzymes (aces) are key components of the renin-angiotensin system in mammals. however, the function of ace homologs in insect saliva is unclear. aphids presumably deliver effector proteins via saliva into plant cells to maintain a compatible insect-plant interaction. in this study, we showed that ace modulates aphid-plant interactions by affecting feeding behavior and survival of aphids on host plants. three ace genes were identified from the pea aphid acyrthosiphon pisum ...	2015	25744345
does the aphid alarm pheromone (e)-β-farnesene act as a kairomone under field conditions?	insect natural enemies use several environmental cues for host/prey finding, and adjust their foraging behavior according to these signals. in insects, such cues are mainly chemical, derived from the host plant or the prey itself. the aphid alarm pheromone, (e)-β-farnesene (ebf), is believed to be such a cue, because several aphid enemies are able to perceive ebf and show attractant behavior. these studies are, however, based mainly on electroantennogram or olfactometer assays, and often use unn ...	2015	25779875
cascading effects of artificial light at night: resource-mediated control of herbivores in a grassland ecosystem.	artificial light at night has a wide range of biological effects on both plants and animals. here, we review mechanisms by which artificial light at night may restructure ecological communities by modifying the interactions between species. such mechanisms may be top-down (predator, parasite or grazer controlled), bottom-up (resource-controlled) or involve non-trophic processes, such as pollination, seed dispersal or competition. we present results from an experiment investigating both top-down ...	2015	25780243
bacterial communities associated with host-adapted populations of pea aphids revealed by deep sequencing of 16s ribosomal dna.	associations between microbes and animals are ubiquitous and hosts may benefit from harbouring microbial communities through improved resource exploitation or resistance to environmental stress. the pea aphid, acyrthosiphon pisum, is the host of heritable bacterial symbionts, including the obligate endosymbiont buchnera aphidicola and several facultative symbionts. while obligate symbionts supply aphids with key nutrients, facultative symbionts influence their hosts in many ways such as protecti ...	2015	25807173
infection dynamic of symbiotic bacteria in the pea aphid acyrthosiphon pisum gut and host immune response at the early steps in the infection process.	in addition to its obligatory symbiont buchnera aphidicola, the pea aphid acyrthosiphon pisum can harbor several facultative bacterial symbionts which can be mutualistic in the context of various ecological interactions. belonging to a genus where many members have been described as pathogen in invertebrates, serratia symbiotica is one of the most common facultative partners found in aphids. the recent discovery of strains able to grow outside their host allowed us to simulate environmental acqu ...	2015	25811863
peroxiredoxin 1 protects the pea aphid acyrthosiphon pisum from oxidative stress induced by micrococcus luteus infection.	reactive oxygen species (ross) are generated in organisms in response to infections caused by invading microbes. however, excessive ross will inflict oxidative damage on the host. peroxiredoxins (prxs) are antioxidative enzymes that may eliminate ross efficiently. in this study, apprx1 from the pea aphid acyrthosiphon pisum was cloned, and its function was investigated in vitro and in vivo. in the presence of dtt, recombinant apprx1 protein from escherichia coli showed antioxidative activity by ...	2015	25817695
molecular cloning and expression profile of an atp-binding cassette (abc) transporter gene from the hemipteran insect nilaparvata lugens.	the atp-binding cassette (abc) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. in insects, abc transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. in this study, the nilaparvata lugens stal (hemiptera: delphacidae) abcg (nlabcg) gene was identified and characterized. the complete mrna sequence of nlabcg was 2608-bp long, with a ...	2015	25867414
house fly (musca domestica l.) attraction to insect honeydew.	house flies are of major concern as vectors of food-borne pathogens to food crops. house flies are common pests on cattle feedlots and dairies, where they develop in and feed on animal waste. by contacting animal waste, house flies can acquire human pathogenic bacteria such as escherichia coli and salmonella spp., in addition to other bacteria, viruses, or parasites that may infect humans and animals. the subsequent dispersal of house flies from animal facilities to nearby agricultural fields co ...	2015	25970333
the molecular correlates of organ loss: the case of insect malpighian tubules.	malpighian tubules play an essential role in excretion, osmoregulation and immunity of most insects. exceptionally, aphids lack malpighian tubules, providing the opportunity to investigate the fate of genes expressed in an organ that has undergone evolutionary reduction and loss. making use of the sequenced genomes of drosophila melanogaster and the pea aphid acyrthosiphon pisum, we demonstrated that more than 50% of drosophila genes expressed specifically in the malpighian tubules had orthologu ...	2015	25972400
genome sequence of an alphabaculovirus isolated from the oak looper, lambdina fiscellaria, contains a putative 2-kilobase-pair transposable element encoding a transposase and a flywch domain-containing protein.	the genome sequence of an alphabaculovirus isolated from lambdina fiscellaria indicated that it is a novel member of a group ii lineage. a putative transposable element was identified that contained two genes, including a transposase ortholog. these genes were most closely related to genes of the pea aphid, acyrthosiphon pisum.	2015	26021909
proton-dependent glutamine uptake by aphid bacteriocyte amino acid transporter apglnt1.	aphids house large populations of the gammaproteobacterial symbiont buchnera aphidicola in specialized bacteriocyte cells. the combined biosynthetic capability of the holobiont (acyrthosiphon pisum and buchnera) is sufficient for biosynthesis of all twenty protein coding amino acids, including amino acids that animals alone cannot synthesize; and that are present at low concentrations in a. pisum's plant phloem sap diet. collaborative holobiont amino acid biosynthesis depends on glutamine import ...	2015	26028424
feeding history affects intraguild interactions between harmonia axyridis (coleoptera: coccinellidae) and episyrphus balteatus (diptera: syrphidae).	while the effect of several factors such as predator and prey size, morphology and developmental stage on intraguild predation (igp) is widely investigated, little is known about the influence of diet on the occurrence and outcome of igp. in the present study, the effect of the diet experienced during larval development on igp between the ladybird harmonia axyridis and the syrphid episyrphus balteatus is investigated. four diets were tested for h. axyridis: eggs of the mediterranean flour moth e ...	2015	26030267
the genome of diuraphis noxia, a global aphid pest of small grains.	the russian wheat aphid, diuraphis noxia kurdjumov, is one of the most important pests of small grains throughout the temperate regions of the world. this phytotoxic aphid causes severe systemic damage symptoms in wheat, barley, and other small grains as a direct result of the salivary proteins it injects into the plant while feeding.	2015	26044338
rna interference against gut osmoregulatory genes in phloem-feeding insects.	in planta rnai (i.e. plants engineered to synthesize active rnai molecules) has great potential as a strategy to control insect crop pests. this study investigated the impact of rnai against osmoregulatory genes expressed in the gut of two phloem-feeding species, the green peach aphid myzus persicae and the potato/tomato psyllid bactericera cockerelli. the target genes comprising candidate gut sucrase, aquaporin and sugar transporter genes were identified by mining insect genomic and transcripto ...	2015	26071792
rhodnius prolixus supergene families of enzymes potentially associated with insecticide resistance.	chagas disease or american trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, trypanosoma cruzi. once known as an endemic health problem of poor rural populations in latin american countries, it has now spread worldwide. the parasite is transmitted by triatomine bugs, of which rhodnius prolixus (hemiptera, reduviidae, triatominae) is one of the vectors and a model organism. this species occurs mainly in central and south american countries where the dise ...	2016	26079630
egg predation by the introduced lady beetle, coccinella septempunctata (coleoptera: coccinellidae), lowers mortality but raises relative risk for the native lady beetle, coccinella novemnotata.	populations of the native ninespotted lady beetle, coccinella novemnotata herbst, have undergone precipitous declines in north america following the establishment of the exotic sevenspotted lady beetle, coccinella septempunctata l. recent volunteer efforts have made it possible to establish colonies of the now-rare c. novemnotata and test mechanisms contributing to its decline. we evaluated the relative frequencies of intraguild predation and cannibalism of eggs between these two species. a sing ...	2015	26090935
evidence for specificity in symbiont-conferred protection against parasitoids.	many insects harbour facultative symbiotic bacteria, some of which have been shown to provide resistance against natural enemies. one of the best-known protective symbionts is hamiltonella defensa, which in pea aphid (acyrthosiphon pisum) confers resistance against attack by parasitoid wasps in the genus aphidius (braconidae).we asked (i) whether this symbiont also confers protection against a phylogenetically distant group of parasitoids (aphelinidae) and (ii) whether there are consistent diffe ...	2015	26136451
circulative nonpropagative aphid transmission of nanoviruses: an oversimplified view.	plant virus species of the family nanoviridae have segmented genomes with the highest known number of segments encapsidated individually. they thus likely represent the most extreme case of the so-called multipartite, or multicomponent, viruses. all species of the family are believed to be transmitted in a circulative nonpropagative manner by aphid vectors, meaning that the virus simply crosses cellular barriers within the aphid body, from the gut to the salivary glands, without replicating or e ...	2015	26178991
a facultative endosymbiont in aphids can provide diverse ecological benefits.	ecologically important traits of insects are often affected by facultative bacterial endosymbionts. this is best studied in the pea aphid acyrthosiphon pisum, which is frequently infected by one or more of eight facultative symbiont species. many of these symbiont species have been shown to provide one ecological benefit, but we have little understanding of the range of effects that a single strain can have. here, we describe the phenotypes conferred by three strains of the recently discovered b ...	2015	26206380
coping with shorter days: do phenology shifts constrain aphid fitness?	climate change can alter the phenology of organisms. it may thus lead seasonal organisms to face different day lengths than in the past, and the fitness consequences of these changes are as yet unclear. to study such effects, we used the pea aphid acyrthosiphon pisum as a model organism, as it has obligately asexual clones which can be used to study day length effects without eliciting a seasonal response. we recorded life-history traits under short and long days, both with two realistic tempera ...	2015	26207194
g protein coupled receptors as targets for next generation pesticides.	there is an on-going need for the discovery and development of new pesticides due to the loss of existing products through the continuing development of resistance, the desire for products with more favourable environmental and toxicological profiles and the need to implement the principles of integrated pest management. insect g protein coupled receptors (gpcrs) have important roles in modulating biology, physiology and behaviour, including reproduction, osmoregulation, growth and development. ...	2015	26226649
transcription factors, chromatin proteins and the diversification of hemiptera.	availability of complete genomes provides a means to explore the evolution of enormous developmental, morphological, and behavioral diversity among insects. hemipterans in particular show great diversity of both morphology and life history within a single order. to better understand the role of transcription regulators in the diversification of hemipterans, using sequence profile searches and hidden markov models we computationally analyzed transcription factors (tfs) and chromatin proteins (cps ...	2016	26226651
pea aphid infestation induces changes in flavonoids, antioxidative defence, soluble sugars and sugar transporter expression in leaves of pea seedlings.	the perception of aphid infestation induces highly coordinated and sequential defensive reactions in plants at the cellular and molecular levels. the aim of the study was to explore kinetics of induced antioxidative defence responses in leaf cells of pisum sativum l.cv. cysterski upon infestation of the pea aphid acyrthosiphon pisum at varying population sizes, including accumulation of flavonoids, changes of carbon metabolism, and expression of nuclear genes involved in sugar transport. within ...	2016	26239447
host-associated differentiation in a highly polyphagous, sexually reproducing insect herbivore.	insect herbivores may undergo genetic divergence on their host plants through host-associated differentiation (had). much of what we know about had involves insect species with narrow host ranges (i.e., specialists) that spend part or all their life cycle inside their hosts, and/or reproduce asexually (e.g., parthenogenetic insects), all of which are thought to facilitate had. however, sexually reproducing polyphagous insects can also exhibit had. few sexually reproducing insects have been teste ...	2015	26257868
parasitic wasps aphidius ervi are more attracted to a blend of host-induced plant volatiles than to the independent compounds.	arthropodal natural enemies respond to volatiles from plants infested by their prey/host herbivores (herbivore-induced plant volatiles; hipvs). however, the relative importance of hipv blends vs. each compound in the blend in attracting natural enemies is not fully understood. in this study, we investigated the response of a parasitic wasp, aphidius ervi, to hipvs that were specific or nonspecific to infestations by its host aphid, acyrthosiphon pisum. to select such compounds, we compared the v ...	2015	26302986
in vitro evidence supports membrane alanyl aminopeptidase n as a receptor for a plant virus in the pea aphid vector.	insect-borne plant viruses cause significant agricultural losses and jeopardize sustainable global food production. although blocking plant virus transmission would allow for crop protection, virus receptors in insect vectors are unknown. here we identify membrane alanyl aminopeptidase n (apn) as a receptor for pea enation mosaic virus (pemv) coat protein (cp) in the gut of the pea aphid, acyrthosiphon pisum, using a far-western blot method. pulldown and immunofluorescence binding assays and sur ...	2015	26311872
do bacterial symbionts govern aphid's dropping behavior?	defensive symbiosis is amongst nature's most important interactions shaping the ecology and evolution of all partners involved. the pea aphid, acyrthosiphon pisum harris (hemiptera: aphididae), harbors one obligatory bacterial symbiont and up to seven different facultative symbionts, some of which are known to protect the aphid from pathogens, natural enemies, and other mortality factors. pea aphids typically drop off the plant when a mammalian herbivore approaches it to avoid incidental predati ...	2015	26313964
horizontal transfer of facultative endosymbionts is limited by host relatedness.	heritable microbial symbionts can have important effects on many aspects of their hosts' biology. acquisition of a novel symbiont strain can provide fitness benefits to the host, with significant ecological and evolutionary consequences. we measured barriers to horizontal transmission by artificially transferring facultative symbionts from the grain aphid, sitobion avenae, and five other aphid species into two clonal genotypes of s. avenae. we found the symbiont hamiltonella defensa establishes ...	2015	26332792
injection of insect membrane in xenopus oocyte: an original method for the pharmacological characterization of neonicotinoid insecticides.	insect nicotinic acetylcholine receptors (nachrs) represent a major target of insecticides, belonging to the neonicotinoid family. however, the pharmacological profile of native nachrs is poorly documented, mainly because of a lack of knowledge of their subunit stoichiometry, their tissue distribution and the weak access to nachr-expressing cells. in addition, the expression of insect nachrs in heterologous systems remains hard to achieve. therefore, the structure-activity characterization of na ...	2015	26391340
germ plasm localisation of the helicc of vasa in drosophila: analysis of domain sufficiency and amino acids critical for localisation.	formation of the germ plasm drives germline specification in drosophila and some other insects such as aphids. identification of the dead-box protein vasa (vas) as a conserved germline marker in flies and aphids suggests that they share common components for assembling the germ plasm. however, to which extent the assembly order is conserved and the correlation between functions and sequences of vas remain unclear. ectopic expression of the pea aphid vas (apvas1) in drosophila did not drive its l ...	2015	26419889
drawing the line.	the pea aphid acyrthosiphon pisum is an agriculturally important pest of leguminous plants including peas and broad beans. the widespread use of chemical pesticides impacts heavily on the environment, and increases pesticide-resistant pea aphid populations, so alternative strategies are being actively sought. pseudomonas syringae bacteria are known to infect and kill the pea aphid, and offer a possible control strategy. in this study, the authors measured the effects of injecting p. syringae on ...	2015	26438421
automated solution-phase synthesis of insect glycans to probe the binding affinity of pea enation mosaic virus.	pea enation mosaic virus (pemv)--a plant rna virus transmitted exclusively by aphids--causes disease in multiple food crops. however, the aphid-virus interactions required for disease transmission are poorly understood. for virus transmission, pemv binds to a heavily glycosylated receptor aminopeptidase n in the pea aphid gut and is transcytosed across the gut epithelium into the aphid body cavity prior to release in saliva as the aphid feeds. to investigate the role of glycans in pemv-aphid int ...	2015	26457763
effect of host genotype on symbiont titer in the aphid-buchnera symbiosis.	obligate nutritional symbioses require balance between the energetic needs of the host and the symbiont. the resident symbiont population size within a host may have major impacts on host fitness, as both host and symbiont consume and supply metabolites in a shared metabolite pool. given the massive genome degradation that is a hallmark of bacterial endosymbionts of insects, it is unclear at what level these populations are regulated, and how regulation varies among hosts within natural populati ...	2011	26467737
morphology and proteome characterization of the salivary glands of the western chinch bug (hemiptera: blissidae).	the western chinch bug, blissus occiduus barber, is a serious pest of buffalograss, buchloe dactyloides (nuttall) due to physical and chemical damage caused during the feeding process. although previous work has investigated the feeding behaviors of chinch bugs in the blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. whole and sectioned b. occiduus salivary glands were visualized using light and scanning electron microsco ...	2015	26470353
enemy-free space promotes maintenance of host races in an aphid species.	the enormous biodiversity of herbivorous insects may arise from ecological speciation via continuous host-plant switches. whether such switches are successful depends on the trade-off between different selection pressures that act on herbivores. decreased herbivore performance due to suboptimal nutrition might be compensated for by a reduced natural enemy pressure. as a consequence, an "enemy-free space" on a certain plant might facilitate host-plant switches and maintain biotypes. to test this ...	2016	26520659
protection of pea aphids associated with coinfecting bacterial symbionts persists during superparasitism by a braconid wasp.	bacterial endosymbionts that associate facultatively with insect herbivores can influence insect fitness and trophic interactions. the pea aphid, acyrthosiphon pisum, can be protected from parasitism by the braconid wasp aphidius ervi when harbouring particular symbiotic bacteria, with specific endosymbiont coinfections providing almost complete protection. however, studies often quantify aphid mummification with no control over parasitoid oviposition per aphid; thus, if mummy production fails o ...	2016	26520831
up-regulation of abscisic acid signaling pathway facilitates aphid xylem absorption and osmoregulation under drought stress.	the activation of the abscisic acid (aba) signaling pathway reduces water loss from plants challenged by drought stress. the effect of drought-induced aba signaling on the defense and nutrition allocation of plants is largely unknown. we postulated that these changes can affect herbivorous insects. we studied the effects of drought on different feeding stages of pea aphids in the wild-type a17 of medicago truncatula and aba signaling pathway mutant sta-1. we examined the impact of drought on pla ...	2015	26546578
the x chromosome of hemipteran insects: conservation, dosage compensation and sex-biased expression.	insects of the order hemiptera (true bugs) use a wide range of mechanisms of sex determination, including genetic sex determination, paternal genome elimination, and haplodiploidy. genetic sex determination, the prevalent mode, is generally controlled by a pair of xy sex chromosomes or by an xx/x0 system, but different configurations that include additional sex chromosomes are also present. although this diversity of sex determining systems has been extensively studied at the cytogenetic level, ...	2015	26556591
ants learn aphid species as mutualistic partners: is the learning behavior species-specific?	in ant-aphid associations, many aphid species provide ants with honeydew and are tended by ants, whereas others are never tended and are frequently preyed upon by ants. in these relationships, ants must have the ability to discriminate among aphid species, with mutualistic aphids being accepted as partners rather than prey. although ants reportedly use cuticular hydrocarbons (chcs) of aphids to differentiate between mutualistic and non-mutualistic species, it is unclear whether the ability to re ...	2015	26590597
conditional reduction of predation risk associated with a facultative symbiont in an insect.	symbionts are widespread among eukaryotes and their impacts on the ecology and evolution of their hosts are meaningful. most insects harbour obligate and facultative symbiotic bacteria that can influence their phenotype. in the pea aphid acyrthosiphon pisum, an astounding symbiotic-mediated phenotype has been recently observed: when infected with the symbiotic bacteria rickettsiella viridis, young red aphid larvae become greener at adulthood and even darker green when co-infected with rickettsie ...	2015	26618776
stable isotope studies reveal pathways for the incorporation of non-essential amino acids in acyrthosiphon pisum (pea aphids).	plant roots incorporate inorganic nitrogen into the amino acids glutamine, glutamic acid, asparagine and aspartic acid, which together serve as the primary metabolites of nitrogen transport to other tissues. given the preponderance of these four amino acids, phloem sap is a nutritionally unbalanced diet for phloem-feeding insects. therefore, aphids and other phloem feeders typically rely on microbial symbionts for the synthesis of essential amino acids. to investigate the metabolism of the four ...	2015	26632455
bacteria may contribute to distant species recognition in ant-aphid mutualistic relationships.	mutualistic interactions between ant and aphid species have been the subject of considerable historical and contemporary investigations, the primary benefits being cleaning and protection for the aphids and carbohydrate-rich honeydew for the ants. questions remained, however, as to the volatile semiochemical factor influencing this relationship. a recent study highlighted the role of bacterial honeydew volatile compounds in ant attraction. here, ant's ability to distantly discriminate 2 aphid sp ...	2017	26639575
condition-dependent alteration of cellular immunity by secondary symbionts in the pea aphid, acyrthosiphon pisum.	endosymbionts can fundamentally alter host physiology. whether such changes are beneficial or detrimental to one or both partners may depend on the dynamics of the symbiotic relationship. here we investigate the relationship between facultative symbionts and host immune responses. the pea aphid, acyrthosiphon pisum, maintains an obligate primary symbiont, but may also harbour one or more facultative, secondary symbionts. given their more transient nature and relatively recent adoption of a symbi ...	2016	26699661
performances of survival, feeding behavior, and gene expression in aphids reveal their different fitness to host alteration.	insect populations feeding on different plant species are under selection pressure to adapt to these differences. a study integrating elements of the ecology, behavior, and gene expression of aphids on different host plants has not yet been well-explored. the present study explores the relationship between host fitness and survival, feeding behavior, and salivary gland gene expression of a pea (pisum sativum) host race of acyrthosiphon pisum feeding on a common host vicia faba and on three genet ...	2016	26758247
ontogenetic differences in localization of glutamine transporter apglnt1 in the pea aphid demonstrate that mechanisms of host/symbiont integration are not similar in the maternal versus embryonic bacteriome.	obligate intracellular symbionts of insects are metabolically and developmentally integrated with their hosts. typically, reproduction fails in many insect nutritional endosymbioses when host insects are cured of their bacterial symbionts, and yet remarkably little is known about the processes that developmentally integrate host and symbiont. here in the best studied insect obligate intracellular symbiosis, that of the pea aphid, acyrthosiphon pisum, with the gammaproteobacterium buchnera aphidi ...	2016	26759710
diversity, frequency and geographic distribution of facultative bacterial endosymbionts in introduced aphid pests.	facultative bacterial endosymbionts in insects have been under intense study during the last years. endosymbionts can modify the insect's phenotype, conferring adaptive advantages under environmental stress. this seems particularly relevant for a group of worldwide agricultural aphid pests, because endosymbionts modify key fitness related traits, including host plant use, protection against natural enemies and heat tolerance. aimed to understand the role of facultative endosymbionts on the succe ...	2016	26773849
determination of melatonin in acyrthosiphon pisum aphids by liquid chromatography-tandem mass spectrometry.	melatonin is a hormone mainly involved in the regulation of circadian and seasonal rhythms in both invertebrates and vertebrates. despite the identification of melatonin in many insects, its involvement in the insect seasonal response remains unclear. a liquid chromatography tandem mass spectrometry (lc-ms/ms) method has been developed for melatonin analysis in aphids (acyrthosiphon pisum) for the first time. after comparing two different procedures and five extraction solvents, a sample prepara ...	2016	26778054
synthesis of new dicinnamoyl 4-deoxy quinic acid and methyl ester derivatives and evaluation of the toxicity against the pea aphid acyrthosiphon pisum.	new dicinnamoyl (caffeoyl, feruloyl, ortho and para-coumaroyl) 4-deoxyquinic acid and esters were synthesized by using a new 4-deoxy quinic acid triol intermediate. the optimisation of both coupling and deprotection steps allowed the preparation in good yields of the target products either as the carboxylic acid or the methyl ester form. eight new compounds were evaluated for their ability to influence the feeding behaviour of the pea aphid acyrthosiphon pisum. artificial diet bioassays showed t ...	2016	26815337
direct flow cytometry measurements reveal a fine-tuning of symbiotic cell dynamics according to the host developmental needs in aphid symbiosis.	endosymbiotic associations constitute a driving force in the ecological and evolutionary diversification of metazoan organisms. little is known about whether and how symbiotic cells are coordinated according to host physiology. here, we use the nutritional symbiosis between the insect pest, acyrthosiphon pisum, and its obligate symbiont, buchnera aphidicola, as a model system. we have developed a novel approach for unculturable bacteria, based on flow cytometry, and used this method to estimate ...	2016	26822159
identification of critical conditions for immunostaining in the pea aphid embryos: increasing tissue permeability and decreasing background staining.	the pea aphid acyrthosiphon pisum, with a sequenced genome and abundant phenotypic plasticity, has become an emerging model for genomic and developmental studies. like other aphids, a. pisum propagate rapidly via parthenogenetic viviparous reproduction, where the embryos develop within egg chambers in an assembly-line fashion in the ovariole. previously we have established a robust platform of whole-mount in situ hybridization allowing detection of mrna expression in the aphid embryos. for analy ...	2016	26862939
deep rna-seq profile reveals biodiversity, plant-microbe interactions and a large family of nbs-lrr resistance genes in walnut (juglans regia) tissues.	deep rna-seq profiling, a revolutionary method used for quantifying transcriptional levels, often includes non-specific transcripts from other co-existing organisms in spite of stringent protocols. using the recently published walnut genome sequence as a filter, we present a broad analysis of the rna-seq derived transcriptome profiles obtained from twenty different tissues to extract the biodiversity and possible plant-microbe interactions in the walnut ecosystem in california. since the residua ...	2016	26883051
evolutionary conservation of candidate osmoregulation genes in plant phloem sap-feeding insects.	the high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. the genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid acyrthosiphon pisum: sucrase 1 (suc1), a sucrase in glycoside hydrolase family 13 (gh13), and aquaporin 1 (aqp1), a member of the drosophila integral protein (drip) family of aquaporins. here, we describe molecular anal ...	2016	26896054
insulin-related peptide 5 is involved in regulating embryo development and biochemical composition in pea aphid with wing polyphenism.	in aphids there is a fecundity-dispersal trade-off between wingless and winged morphs. recent research on the molecular mechanism of wing morphs associated with dispersal reveals that insulin receptors in the insulin signaling (is) pathway regulate alternation of wing morphs in planthoppers. however, little is known about whether genes in the is pathway are involved in developmental regulation in aphid nymphs with different wing morphs. in this study, we show that expression of the insulin-relat ...	2016	26903881
de novo transcriptome assembly of the grapevine phylloxera allows identification of genes differentially expressed between leaf- and root-feeding forms.	grapevine phylloxera, an insect related to true aphids, is a major historic pest of viticulture only controlled through the selection of resistant rootstocks or through quarantine regulations where grapevine is cultivated own-rooted. transcriptomic data could help understand the bases of its original life-traits, including a striking case of polyphenism, with forms feeding on roots and forms feeding in leaf-galls. comparisons with true aphids (for which complete genomes have been sequenced) shou ...	2016	26968158