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application of isoelectric focusing to the taxonomic identification of meloidogyne spp.meloidogyne incognita, m. arenaria, m. hapla, and m. javanica were distinguishable from each other by isoelectric focusing (ief) of nematode egg proteins. proteins extracted from larvae and adults of hoplolaimus columbus and from eggs of heterodera glycines had distinctive profiles, also. protein profiles from eggs, preparasitic larvae and egg-laying adults of m. incognita showed differences. it was necessary to compare samples run at the same time to ensure reliability.198419295881
nematicides and nonconventional soil amendments in the management of root-knot nematode on cotton.granular and liquid commercial humates, with micronutrients, and a microbial fermentation product were compared in several combinations with nematicides for their effects on cotton lint yield and root-knot nematode suppression. fumigant nematicides effectively reduced cotton root galling caused by root-knot nematodes, and cotton lint yields increased. organophosphates and carbamates were not effective. occasionally, cotton lint yields were increased or maintained with combination treatments o f ...198419295893
reactions of grape rootstocks to pratylenchus vulnus and meloidogyne spp.five grape rootstocks were inoculated with 0, 100, 1,000, and 10,000 pratylenchus vulnus. dogridge and saltcreek supported low average total numbers of p. vulnus, 136-705/pot, at 12 months after inoculation. growth of both rootstocks was not affected. harmony, couderc 1613, and ganzin 1 supported high average total numbers, 6-856 times the inoculum levels. numbers in harmony continued to increase at all levels but reduced root weight only at the 10,000 level after 12 months. numbers in couderc 1 ...198419295896
histochemical localization and nematoxicity of terpenoid aldehydes in cotton.in healthy cotton, except for random occasional occurrence in cortical cells, terpenoid aldehydes (ta) are localized in the epidermis and, even there, are absent from the tip 2-4 cm of the root. since constitutive ta do not occur in the endodermis and stele of the root, they cannot be effective agents against the development of the sedentary stage of the root-knot nematode, meloidogyne incognita. within 4 days after inoculation with the root-knot nematode, infection-induced ta accumulated in the ...197919300641
pathogenicity of macrophomina phaseoli on jute in the presence of meloidogyne incognita and hoplolaimus indicus.seedlings of corchorus capsularis (cv. c4444) were inoculated with meloidogyne incognita, hoplolaimus indicus, and a fungus pathogen of jute, macrophomina phaseoli, separately and in all possible combinations. the significant damage of jute plants caused individually by the pathogens was aggravated when the fungus was associated with either of the nematode species. m. incognita alone caused greater damage than either h. indicus or macrophomina phaseoli alone. plants inoculated with m. incognita ...197919300650
histological study of the compatible and incompatible interaction of soybeans and meloidogyne incognita. 197919300654
response of pinus ponderosa seedlings to stylet-bearing nematodes.of 12 stylet-bearing nematodes used for inoculations, pratylenchus penetrans, p. brachyurus, p. vulnus, ditylenchus destructor, meloidogyne incognita, m. javanica, and m. hapla reproduced on pinus ponderosa, while xiphinema index, aphelenchus avenae, paratylenehus neoamblycephalus, tylenchulus semipenetrans, and macroposthonia xenoplax did not. p. vulnus, p. brachyurus, p. penetrans, a. avenae, d. destructor, t. semipenetrans, and p. neoamblycephalus significantly suppressed both the shoot and r ...197919300659
histological responses of four leguminous crops infected with meloidogyne incognita.histological responses to meloidogyne incognita infection in rhizobium nodules of clover, horsebean, lupine, and pea were investigated. the formation of giant cells in vascular bundles of nodules and roots, and the basal connection of the nodule, were usually associated with abnormal xylem and/or deformed xylem strands. however, giant cells did not disturb or prevent the development of nodular tissues. areas in which galls formed, wall thickness of giant cells, and number of giant cells around t ...197919300661
managing nematode population densities on tomato transplants using crop rotation and a nematicide.millet, milo, soybean, crotalaria, and norman pigeon pea were used in conjunction with clean fallow and a nematicide (fensulfothion) for managing nematode populations in the production of tomato transplants (lycopersicon esculentum mill.). glean fallow was the most effective treatment in suppressing nematode numbers. after 2 years in tomato, root-knot nematodes increased in numbers to damaging levels, and fallow was no longer effective for complete control even in conjunction with fensulfothion. ...198019300664
morphological comparison of meloidogyne males by scanning electron microscopy.field plots of tifton loamy sand were treated with methyl bromide, dd-mencs, or ethoprop for control of root-knot nematodes, meloidogyne incognita, in a multiple cropping system of turnips, field corn, and southern peas. annual applications of methyl bromide and dd-mencs in november or december suppressed nematode numbers to very low levels through september, but numbers increased in the following october, november, and/or december. no benefit was found from ethoprop applied to dd-mencs-treated ...198019300667
interaction of uromyces phaseoli and meloidogyne incognita on bean.uromyces phaseoli, the causal agent of bean rust, suppressed shoot and root growth of three bean cultivars, reducing root weight more than shoot weight. the greatest suppression of root weight was on the cultivar that appeared most susceptible by visual ratings of shoot symptoms. meloidogyne incognita suppressed shoot and root growth of all test cultivars; root weight reductions differed among cultivars identical in susceptibility to this pathogen in root-gall rating tests. infection of plants w ...198019300692
inhibition of syncytia formation and root-knot nematode development on cultures of excised tomato roots.two different defined growth media were used to culture aseptically the root-knot nematode, meloidogyne incognita, on excised roots of tomato, lycopersicon esculentum cv 'marglobe.' one of these media, stw, was a formulation by skoog, tsui, and white and the other, ms, a formulation by murashige and skoog. from 1 through 4 weeks, inoculated tissues were fractured to observe root infection, giant-cell formation, and nematode development with the scanning electron microscope (sem). four weeks afte ...198019300696
effects of one and two applications of nematicides on nematode populations and soybean yields.yields of 'mcnair 800' soybeans, glycine max (l.) merr., were significantly increased with ethylene dibromide + chloropicrin, dbcp, phenamiphos, and aldicarb applied at-planting and with phenamiphos, aldicarb, and dbcp applied postplant to soil infested with meloidogyne incognita (kofoid and white) chitwood. yields of 'gasoy 17' were significantly increased with ethylene dibromide + chloropicrin, dbcp, phenamiphos, and aldicarb applied, preplant and with dbcp, carbofuran, phenamiphos, aldicarb, ...198019300706
effect of foliar application of carbofuran and a related compound on plant-parasitic nematodes under greenhouse and growth chamber conditions.greenhouse and growth chamber studies were made to investigate the downward systemic nematicidal activity of carbofuran and its analog 2,3-dihydro-2,2-dimethyl-7-benzofuranyl [(di-n-butyl)-4-aminosulfenyl] carbamate against meloidogyne incognita, tylenchorhynchus claytoni, and pratylenchus penetrans. oxamyl was used as standard in tests with t. claytoni. both carbofuran and its analog reduced all three plant-parasitic species when plant foliage was sprayed with chemical suspension of 1,200, 2,40 ...198119300716
dynamics of concomitant populations of hoplolaimus columbus, scutellonema brachyurum, and meloidogyne incognita on cotton.cotton seedlings grown in a greenhouse and a growth chamber were inoculated with scutellonema brachyurum, hoplolaimus columbus, and meloidogyne incognita, singly and in all possible combinations, at two initial population (pi) levels (100 and 300/100 cm(3)). s. brachyurum alone was not pathogenic to cotton at these population levels. it fed primarily as an ectoparasite but matured and reproduced within the root when it penetrated. populations of s. brachyurum increased in the presence of h. colu ...198119300720
influence of meloidogyne incognita on fusarium wilt of tomato at or below the minimum temperature for wilt development.'bonny best' tomato plants were grown at 16, 21, or 24 c for 28 d in soil infested with either of two isolates of fusarium oxysporum f. sp. lycopersici race 1 and meloidogyne incognita. significant levels of fusarium wilt occurred at all temperatures including 16 c, which has not been reported previously. one fusarium isolate resulted in the highest levels of disease incidence at 21 and 24 c in the presence of root-knot nematodes, and at 24 c when the nematodes were not present. at 16 c there wa ...198119300723
identification of the chromosome carrying the factor for resistance to meloidogyne incognita in tobacco.to identify the chromosome carrying the factor for resistance to meloidogyne incognita in tobacco, crosses were made between resistant tobacco 'nc95' as pollen parent and each of the 12 tobacco monosomics (a-l) representative of the tomentosae half of the nicotiana tabacum chromosome complement. of the f seedlings, 927 plants were grown for observation. from these, 223 plants were selected as possible monosomics on the basis of morphological characteristics. these plants were self-pollinated, an ...198119300724
oogenesis and the chromosomes of the parthenogenic root-knot nematode meloidogyne incognita.220 populations of meloidogyne incognita and related forms from 46 countries reproduced by mitotic parthenogenesis (apomixis). determination of somatic chromosome numbers from oogonia and oocytes revealed the existence of a predominant, possibly triploid race a with 3n = 40 to 46 and a rare, diploid race b with 2n = 32 to 36 chromosomes. there is no correlation between cytological races and the four recognized host races of this species. the characteristic behavior of prophase i chromosomes of m ...198119300730
effects of film mulch and soil pesticides on nematodes, weeds, and yields of vegetable crops.field plots in tifton loamy sand were treated with various soil pesticides in 1973 and 1974 and either left exposed or covered with biodegradable flint mulch. test crops were cantaloup, slicing and pickling cucumber, squash, and sweet corn. overhead sprinkler irrigation was used in 1973, and trickle irrigation under the film mulch was used on sweet corn in 1974. soil was assayed for nematodes, and roots of plants were evaluated for damage by root-knot nematodes. nematode populations were reduced ...198119300735
influence of water and soil temperature on the concentration and efficacy of phenamiphos for control of root-knot nematodes.field plots of tifton loamy sand were treated with phenamiphos for control of root-knot nematodes in a multiple-crop system of turnips, field corn, and southern peas. preplant applications of phenamiphos protected roots of turnips and corn from damage by root-knot nematodes. concentrations of phenamiphos at application in the 0-15-cm soil layer were near 6 mug/g on turnips and near 4 mug/g on corn and southern peas. after 30 d, concentrations were approximately 1 mug phenamiphos/g of soil for al ...198119300736
applying nematicides through an overhead sprinkler irrigation system for control of nematodes.phenamiphos, ethoprop, and carbofuran each at 6.7 kg a.i./ha were applied to squash, southern pea, and corn via injection into a sprinkler irrigation system. this method was then compared with a conventional application of phenamiphos and ethoprop granules spread on the soil surface and incorporated into the top 15 cm for control of macroposthonia ornata and meloidogyne incognita. nematode populations in the soil and root-gall indices were lower, and yields greater, in treated than in untreated ...198119300737
yield response and injury levels of meloidogyne incognita and m. javanica on the susceptible tobacco 'mcnair 944'.the effects of meloidogyne incognita and m. javanica on a susceptible tobacco (nicotiana tabacum l.) cv. mcnair 944 were investigated in field microplots during 1978 and 1979. three initial inoculum levels-4, 16, and 64 nematode eggs and/or second-stage larvae per 100 cm(3) of soil-were used for each nematode species. data obtained from the experiments included plant yield and the amount of reproduction of the two nematode species. at comparative inoculum levels, m. javanica was more aggressive ...198119300744
effect of soil texture and the clay component on migration of meloidogyne incognita second-stage juveniles.the vertical migration of m. incognita juveniles introduced at 20 cm from the roots was studied in five natural soils, 100% silica sand, 95% silica sand with 5% clay, 90% silica sand with 10% clay, and 95% silica sand with 5% clay as a concentrated layer. in natural soils the percentage of juveniles capable of migrating 20 cm and penetrating the roots decreased when the percentage of clay and silt increased. no migration occurred in silica sand without clay particles; when 5 or 10% of clay were ...198119300747
influence of photoperiod and temperature on migrations of meloidogyne juveniles.photoperiod influences the migration of m. incognita juveniles toward tomato roots. approximately 33% migrated vertically 20 cm in 7 days to roots when 12 h dark were alternated with 12 h light. only 7% migrated when light was constant for 24 h. vertical migration of m. incognita juveniles was studied at 14, 16, 18, 20, and 22 c. the migration of m. incognita juveniles begins at about 18 c and reaches its maximum at 22 c. the migration of m. hapla and m. incognita juveniles were compared at 14, ...198119300748
effects of soil temperature and planting date of wheat on meloidogyne incognita reproduction, soil populations, and grain yield.wheat cultivars anza and produra grown in winter in california were planted in meloidogyne incognita infested and noninfested sandy loam plots in october (soil temperature 21 c) and november (soil temperature 16 c) of 1979. meloidogyne incognita penetrated roots of mid-october planted ataza (427 juveniles/g root), developed into adult females by january, and produced 75 eggs/g root by harvest in april. penetration and development did not occur in late plantings. anza seedlings grown in infested ...198119300773
the development and influence of meloidogyne incognita and m. javanica on wheat.the effects of soil temperature and initial inoculum density (pi) of meloidogyne incognito and m. javanica on growth of wheat (triticum aestivum cv. anza) and nematode reproduction were studied in controlled temperature baths in the glasshouse. nematode reproduction was directly proportional to temperature between 14 and 30 c for m. incognita and between 18 and 26 c for m. javanica. reproduction rates (pf/pi, where pf = final number of eggs) for pi's of 3,000, 9,000, and 30,000 eggs/plant were g ...198119300774
scanning electron microscope study of the root-knot nematode (meloidogyne incognita) on tomato root.this study examines the types of structural information that can be gained by utilizing the scanning electron microscope (sem) and a cryofracture technique to examine the host-parasite interaction. roots of tomato, lycopersicon esculentum cv. marglobe, were cultured aseptically and inoculated with the root-knot nematode, meloidogyne incognita. twenty-four hours to four weeks after inoculation, developing galls were removed from the cultures and processed for sem observation. the cryofracture tec ...198119300776
the effect of meloidogyne incognita and tissue wounding on severity of seedling disease of cotton caused by rhizoctonia solani. 198119300778
beta-glycosidases from meloidogyne incognita and m. javanica. 198119300785
foliar spray effects of selected amino acids on sunflower infected with meloidogyne incognita. 198119300787
a soil-free system for assaying nematicidal activity of chemicals.a biological assay system for studying the nematicidal activity of chemicals has been devised using a model consisting of cucumber (cucumis sativus l. cv. long marketer) seedlings growing in the dispo(r) growth-pouch apparatus. meloidogyne incognita was used as the test organism. the response was quantified in terms of the numbers of galls produced. statistical procedures were applied to estimate the ed(50) values of currently available nematicides. this system permits accurate quantification of ...198119300800
meloidogyne megatyla n. sp. a root-knot nematode from loblolly pine.meloidogyne megatyla n. sp. is described from pinus taeda in north carolina. stylet knobs are distinctively high in proportion to width, giving an especially massive appearance to the knobs of larvae and males. mean larval length is 416 mum and stylet length is 14.6 mum. the perineal pattern is composed of smooth striae, with a high arch, and is often somewhat rectangular. the relationship of m. megatyla to other meloidogyne species is unclear, although a comparison is made with meloidogyne inco ...197919305528
the influence of temperature on meloidogyne incognita on soybean.the effects of temperature and initial inoculum density of meloidogyne incognita on soybean growth and nematode reproduction were investigated in greenhouse temperature tanks and in controlled-growth chambers. the interactions of initial inoculum density (p(i)) and soil temperature in effects on shoot growth were adequately described by multiple-regression models. at the highest temperatures (30 or 32/28 c), moderate to high inoculum killed many plants. a p(i) of 27,000 eggs/15-cm-diam pot retar ...197919305530
effect of oryzalin and 1,1-dimethylpiperidinium chloride on cotton and tomato roots infected with the root-knot nematode, meloidogyne incognita.oryzalin (3,5-dinitro-n4,n4-dipropyl-sulfanilamide) and bas 083 (l,l-dimethylpiperdinium chloride) reduced root-knot infection in tomato roots when respectively applied as a soil drench at 20 ppm and 10,000 ppm. oryzalin reduced knot counts with various intervals between treatment and inoculation. bas 083 reduced knot counts only when applied before inoculation. oryzalin was shown not to be a contact nematicide, and bas 083 was only a weak one. neither compound reduced penetration by infective l ...197919305533
effects of management practices on nematode and fungus populations and cucumber yield.three crops of cucumber were grown in succession in beds by use of trickle irrigation, plastic film mulch, and soil chemical treatments over a 17-month period, including a fallow winter season. total yield for the three crops was highest (1208 quintals/ha) in film-mulched plots treated with mbr-cp, and next-highest in film-mulched plots treated with dd-mencs (1094 quintals/ha); total yield was only 456 quintals/ha in film-mulched control (untreated) plots. yield in untreated film-mulched plots w ...197919305534
interaction of population levels of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita on cotton.in autoclaved greenhouse soil without fusarium oxysporum f. sp. vasinfectum, meloidogyne incognita did not cause leaf or vascular discoloration of 59-day-old cotton plants. plants had root galls with as few as 50 meloidogyne larvae per plant. root galling was directly proportional to the initial nematode population level. fusarium wilt symptoms occurred without nematodes with 77,000 fungus propagules or more per gram of soil. as few as 50 meloidogyne larvae accompanying 650 fungus propagules cau ...197919305546
chemical control of nematodes and soil-borne plant-pathogenic fungi on cabbage tranplants.six general-purpose fumigants and one fungicide were applied by different methods and evaluated for control of nematode-fungus complexes on cabbage grown for transplant production. all chemicals reduced populations of nematodes and soil-borne fungi but varied greatly in effectiveness. methyl bromide + chloropicrin (98% methyl bromide + 2% chloropicrin) (mbr-cp gas), dd + methyl isothiocyanate (dd-mencs), methyl bromide + chloropicrin (67% methyl bromide + 31.75% cbloropicrin) (mbr-cp gel), and c ...197919305547
granular nematicides as adjuncts to fumigants for control of cotton root-knot nematodes.growth and yield of cotton were best with combinations of fumigants and organophosphate and carbamate nematicides. organophosphates or carbamates used alone did not give season-long control of root-knot nematodes. long-term control was poor because the temporary sublethal effects of these materials diminished soon enough lhat the nematodes could reproduce. the nematodes survived the treatments and a year of nonhost culture, and damaged a susceptible host crop 2 years after treatment. no such dam ...197919305548
effects of nematicide placement on nematode populations and soybean yields.four methods of placement of dbcp (l,2-dibromo-3-chloropropane) and a single method of application of ethoprop (0-ethyl s,s-dipropyl phosphorodithioate) wexe compared in each of two areas for control of nematodes on soybeans. one area was a marlboro sand infested with hoplolaimus columbus. the other area was a fuquay loamy sand infested with meloidogne incognita. soybean yields were increased and numbers of h. columbus in the row 0-20 cm deep were decreased similarly by all methods of dbcp appli ...197919305549
effects of meloidogyne incognita on nitrogen fixation in soybean.the effects of a north carolina population of meloidogyne incognita on n fixation on root-knot-susceptible 'lee 68' and moderately resistant 'forrest' soybean were evaluated 50, 75, i00, and 135 days after inoculation with nematodes. nematodes stimulated n fixation in lee 68 by 50 days and in forrest by 75 days. at all other intervals, n fixation was either depressed or unaffected by nematodes. additional observations indicate that the susceptibility of lee 68 is associated with greater rates of ...197919305550
meloidogyne incognita: a metabolic sink. 197719305575
the nature and role of metabolic leakage from root-knot nematode galls and infection by rhizoctonia solani.a severe root rot of tomato caused by meloidogyne incognita and rhizoctonia solani was associated with nutrient mobilization into gall tissue and root exudation. root decay did not develop when root exudates were continuously removed by leaching. when leachates were collected from m. incognita-infected and control roots and applied to roots of tomatoes inoculated with r. solani alone, roots receiving leachates from m. incognita-infected roots developed a severe rot while roots receiving leachate ...197719305577
effects of management practices on nematode and fungi populations and okra yield.okra was grown in field plots of tifton loamy sand naturally infested with the nematodes meloidogyne incognita and criconemoides ornalus and the pathogenic fungi fusarium oxysporum, f. solani, f. roseum, and pythium spp. plots were treated with various soil pesticides and left exposed or covered with biodegradable paper film mulch under trickle irrigation. soil was assayed for nematodes and fungi, and plant roots were examined for root-rot and insect damage. fewer nematodes and fungi generally w ...197719305580
terpenoid aldehydes in cotton roots susceptible and resistant to the root-knot nematode, meloidogyne incognita.we investigated the role of terpenoid aldehydes in the resistance of cotton (gossypium hirsutum) to the root-knot nematode (meloidogyne incognita). three-day-old, root-knot-resistant ('auburn 623') and -susceptible ('deltapine 16') seedlings were inoculated with m. incognita. comparable portions of inoculated and noninoculated roots were harvested 2, 4, 7, and 10 days later. terpenoid aldehydes were extracted, separated by thin-layer chromatography, eluted as their phloroglucinol derivatives, an ...197719305600
a comparison of two methods of synchronous inoculation of cotton seedlings with meloidogyne incognita. 197719305605
seed treatment as a means of preventing nematode damage to crop plants.a procedure for treating crop seeds with aqueous solutions of the systemic nematicide oxamyl is described. seedlings from treated seeds were more resistant to attack by parasitic nematodes. leachate from treated seeds reduced the number of free-living nematodes in the surrounding soil.197719305617
peroxidase and 6-phosphogluconate dehydrogenase in resistant and susceptible cotton infected by meloidogyne incognita.assays of specific activities and electrophoretic separations of multiple forms of 6-phosphogluconate dehydrogenase and peroxidase in cotton resistant and susceptible to meloidogyne incognita were conducted 6 days after inoculation. specific activities were greater in infected than in uninfected roots and also were greater in the resistant cultivar, 'clevewilt 6-3-5,' than in the susceptible culti.var, 'm8.' in uninfected roots, peroxidase activity was greater in clevewilt roots than in m8 roots ...197819305810
effects of cultivars, subsoiling, and fumigation on soybean yields and meloidogyne incognita populations.application of 1,2-dibromo-3-chloropropane (dbcp) and subsoiling under the row in tifton sandy loam heavily infested with meloidogyne incognita increased yields of four soybean cultivars. these cultivars have levels of resistance to m. incognita as follows: 'hutton', high; 'essex', intermediate; and 'davis' and 'ransom', low. after growing these four cultivars, sub-soiling, and applying dbcp for 2 years in the same plots, the residual effects of these practices on yield of davis cultivar and pop ...197819305812
anatomical response of grain sorghum roots to meloidogyne incognita acrita.the cotton root-knot nematode, meloidogyne incognita acrita, reproduced on the roots of grain sorghum, causing syncytia in the cortex or stele of lateral roots. giant cells developed either singly with few nuclei or in groups with many nuclei. giant cells that developed in groups appeared the same as those which developed singly. the pericycle and endodermis were interrupted at the site of nematode invasion. large areas of these tissues were absent for one-third of the circumference of the stele ...197819305813
early stages of nematode-induced giant-cell formation in roots of impatiens balsamina.giant cells induced in roots of impatiens balsamina by meloidogyne javanica and meloidogyne incognita have been examined by light and electron microscopy. the first sign of giant-cell formation was division of cells surrounding a larva. cell plate alignment appeared to proceed normally, but cytokinesis was unsuccessful and binucleate cells formed subsequently. no wall breakdown was evident then or later. the number of nuclei appeared to increase by repeated mitosis without separation by cytokine ...197819305816
root-knot nematodes and the process of ageing in plants.infection of plants by root-knot nematodes is often accompanied by physiological changes characteristic of ageing. ultra-low tissue luminescence of infected plants indicated oxidation of cell-membrane lipids. cells with membranes subjected to oxidation lose some of their capacity for water retention. treating tomato and radish with lidocaine hydrochloride, an inhibitor of lipid oxidation, retarded above-ground symptoms of root-knot nematode infection and of ageing.197819305822
scanning electron microscopy of perineal patterns of three species of meloidogyne.the perineal pattern of meloidogyne graminicola is oval, characterized by the anastomosing of cuticular striae converging, and elevating at the tail terminus. a deep lateral incisure forming a low arch traverses the rounded perineal pattern of meloidogyne hapla. punctations at the tail region of m. hapla are not observed with the scanning electron microscope. minute crosslines invaginate with the vulvar lips of meloidogyne incognita. punctate indentations around the anal opening and bordering th ...197819305825
influence of low temperature on rate of development of meloidogyne incognita and m. hapla larvae.development of meloidogyne incognita and m. hapla larvae in clover roots was studied at 20, 16, 12, and 8 c in growth chambers and in the field from fall through spring, in north carolina. larvae of both species invaded roots and developed at 20, 16, and 12 c, but not at 8 c. the time necessary to complete the larval stages at each temperature was determined. the minimal temperature for development of m. incognita larvae was 10.08 c and 8.8 c for m. hapla larvae. in the field, soil temperature a ...197819305832
influence of chilling and freezing temperatures on infectivity of meloidogyne incognita and m. hapla.egg masses and second-stage larvae of meloidogyne incognita and m. hapla in soil were exposed to temperatures ranging from 20 to -8 c. temperature was lowered in 2-day intervals to 16, 12, 8, 4, 0, -4, and -8 c, and the nematodes remained at 4, 0, -4, or -8 c for 18, 14, 10, or 6 days, respectively. unhatched larvae of both species were more resistant to low temperatures than were embryonic stages. within the eggs of m. incognita, 7.5% of embryos and 48% of larval stages survived 14 days at 0 c, ...197819305834
resistant host responses to ten california populations of meloidogyne incognita.resistant and susceptible cultivars of tomato, lima beans, cotton, and alfalfa were tested with 10 populations of meloidogyne incognita from different california locations. nine of the populations differed in aggressiveness on the nine cultivars tested. two populations were especially aggressive toward resistant tomato cultivars.197819305845
influence of soil fumigation on the fusarium-root-knot nematode disease complex of cotton in california.for control of the root-knot nematode, meloidogyne incognita, and the pathogenic wilt fungus, fusarium oxysporum, on cotton, soil fumigants were applied in the field at conventional and higher rates. conventional rates suppressed fusarium wilt but higher rates gave quicker early growth, better stands, less stand loss over the season, a lower percentage of plants infected with wilt, fewer plants with vascular discoloration, and fewer nematodes. the best treatment about doubled the yields of untre ...197819305846
meloidogyne incognita wartellei n. subsp. (meloidogynidae), a root-knot nematode on resistant soybeans in louisiana.meloidogyne incognita wartellei n. subsp, is described and illustrated from roots of soybean (glycine max l.) near washington, louisiana. it is rather limited in distribution in that state, being known at five locations comprising about 60,000 acres. it not only attacks commonly susceptihle soybeans but is a destructive pest on other commercial soybean varieties that are resistant to other forms of the m. incognita group in the area. this new subspecies is related most closely to m. i. incognita ...197819305855
temperature-based prediction of egg-mass production by meloidogyne incognita.a maturation-rate relationship for meloidogyne incognita on lycopersicon esculentum 'rutgers' was derived and used to estimate harvest dates for maximum egg hatch from laboratory cultures at ambient temperatures. daily maturation increments were totaled (nematode maturation total, nmt) and correlated with hatch from isolated white, yellow, and amber egg masses. hatch per mass fluctuated periodically from ca. 1.0 nmt, when egg masses were first visible, to 2.5 nmt by which time plants showed stre ...197819305857
influence of low temperature on development of meloidogyne incognita and m. hapla eggs in egg masses.egg masses of meloidogyne incognita and m. hapla were placed in soil at 10, 12, 16, and 20 c. at regular intervals, eggs from samples of egg masses were released from the gelatinous matrices and their developmental stages recorded. the number of days necessary to complete each stage from gastrulation to hatch is given for each temperature. the minimal temperature threshold for the development of eggs was computed by linear regression to be 8.26 c for m. incognita and 6.74 c for m. hapla.197819305859
amino acids and carbohydrates secreted by meloidogyne incognita. 197819305868
population dynamics of plant nematodes in cultivated soil: effects of combinations of cropping systems and nematicides.the population density of meloidogyne incognita was significantly reduced in land that was fallowed or cropped to crotalaria, marigold, bermudagrass, or bahiagrass. the rate of population decline caused by different cropping systems was influenced by initial population densities. crotalaria, marigold, and bare fallow were about equally effective in reducing the density of m. incognita below dctectable lcvels, usually requiring 1-3 yr. bahiagrass and bcrmudagrass required 4-5 yr or longer to redu ...197419308109
seasonal population dynamics of selected plant-parasitic nematodes on four monocultured crops.seasonal fluctuations in field populations of meloidogyne incognita, pratylenchus zeae, p. brachyurus, criconemoides ornatus, trichodorus christiei, and helicotylenchus dihystera on monocultured corn, cotton, peanut, and soybean were determined monthly for 4 yr. population densities of m. incognita were greater in corn and cotton plots than in peanut and soybean plots from july until january. those of pratylenchus spp. were greater on corn and soybean than on cotton and peanut during all months ...197419308120
dynamics of concomitant field populations of hoplolaimus columbus and meloidogyne incognita.from the fall of 1968 through the summer of 1973, a georgia cotton field with a lengthy history of the cotton stunt disease complex was sampled for the presence of plant parasitic nematodes. although meloidogyne incognita was recovered on all sampling dates, concomitant populations of hoplolaimus columbus were not recovered until the spring of 1970. during the succeeding four growing seasons, the population density and horizontal distribution of h. columbus increased, and h. columbus replaced m. ...197419308121
biochemical changes in root exudate and xylem sap of tomato plants infected with meloidogyne incognita.under two monoxenic culture techniques of growing plants (filter paper and silica sand cultures), sugar in root exudate from meloidogyne incognita-infected tomato increased 133 to 836% over controls. in contrast, amino acids were moderately reduced 52 to 56%. chromatographic analysis showed that galled root exudate contained three sugars, twelve amino acids, and three organic acids, whereas healthy root exudate contained four sugars, fifteen amino acids, and four organic acids. polysaccharide wa ...197419308122
pathogenicity of pratylenchus coffeae, scutellonema bradys, meloidogyne incognita, and rotylenchus reniformis on dioscorea rotundata.low populations (200 specimens per plant) of pratylenchus cofl'eae, scutellonema bradys, meloidogyne incognita, and rotylenchulus reniformis stimulated the development of tops, roots, and tubers of dioscorea rotundata "guinea" yam. we demonstrated experimentally that p. coffeae was responsible for the deterioration in quality of the yam tuber in puerto rico, a condition known as a dry rot of yam. initial populations of 600 p. coffeae, s. bradys, or m. incognita, and populations of 1,000 p. coffe ...197519308127
effect of meloidogyne incognita on reproduction of pratylenchus penetrans in red clover and alfalfa.roots of seedlings of red clover and alfalfa growing on 10(1) hoagland and arnon solution agar were inoculated with various combinations of meloidogyne incognita and pratylenchus penetrans. egg-laying by p. penetrans decreased as the number of nematodes, the ratio of entrant m. incognita to entrant p. penetrans, and the priority of invasion of roots by m. incognita increased. embryogeny and hatching of eggs of p. penetrans, and development of larvae of m. incognita, were not affected. in red clo ...197519308128
free amino acids in roots of infected cotton seedlings resistant and susceptible to meloidogyne incognita.quantities of free amino acids in segments of cotton roots resistant and susceptible to meloidogyne incognita were compared. following infection, the root-knot susceptible cultivar, m8, had greater percentage increases of certain individual free amino acids than the resistant cultivar, clevewilt, but the sum total of free amino acids was greatest in the resistant cultivar. more free amino acids were present in infected than in noninfected plants of both cultivars. the overall concn of glycine de ...197519308129
fine structure of cephalic sense organs in heterodera glycines males.cephalic sense organs of heterodera glycines males were examined in detail by electron microscopy. each amphid basically consists of an amphidial gland, a nerve bundle, and an amphidial duct. the amphidial gland consists of a microvillous region, and laterally is closely associated with a large secretory cell. the nerve bundle penetrates the microvillous region, and further anteriorly individual nerve processes (dendrites) separate from one another, thus forming a sensilla pouch which is envelop ...197519308132
interaction of four soybean cultivars with subsoiling and a nematicide.yields of four soybean, glycine max, cultivars were increased with subsoiling under the row and application of the nematicide, dbcp i 1,2-dibromo-3-chloropropane) in tiflon sandy loam heavily infested with the root-knot nematode meloidogyne incognita. these cultivars represent four maturity groups: very early (v), "essex', early (vi), "davis': medium (vii), 'ransom'; and late (viii), ' hutton '. the average increase for the four cullivars was about the same for subsoiling or dbcp. when the treat ...197519308134
autoradiography of developing syncytia in cotton roots infected with meloidogyne incognita.cotton (gossypium hirsutum) seedlings, uniformly infected with meloidogyne incognita, were exposed for periods of 1-15 days to a nutrient solution containing tritium-labelled thymidine. syncytium formation began with the amalgamation of cells near the nematode head, and was followed by synchronized mitoses of the nuclei which had been incorporated into a single cell. syncytial nuclei synthesized dna in roots harvested 3, 6, 9, 12, and 15 days after inoculation. seedlings transferred from unlabel ...197519308135
marigold, castor bean, and chrysanthemum as controls of meloidogyne incognita and pratylenchus alleni.root and soil populations of meloidogyne incognita were significantly fewer from marigold, castor bean, and chrysanthemum than from tomato roots and soil, but not from fallow soil. root populations of pratvlenchus alleni were significantly fewer from marigold, castor bean, and chrysanthemum than from tomato: marigold had the fewest. root populations of m. incognita and p. alleni from tomato simultaneously cultivated with marigold, castor bean, and chrysanthemum were significantly fewer than from ...197519308137
effect of meloidogyne incognita on selected forest tree species.four or five growth stages of 14 forest tree species were tested for susceptibility to meloidogyne incognita at five inoculum levels. responses ranged from the highly susceptible 'china fir' to immune 'taiwania'. even highly susceptible species became increasingly tolerant at later growth stages, thus root-knot appears to be a greater problem in nurseries than in established forests. heavily suberized cells which restricted nematode development was the predominant host response in norway spruce, ...197519308146
crop rotation and herbicide effects on population densities of plant-parasitic nematodes.the influence of herbicides and mono- and multicropping sequences on population densities of nematode species common in corn, cotton, peanut, and soybean fields in the southeastern united states was studied for 4 years. each experimental plot was sampled at monthly intervals. the application of herbicides did not significantly affect nematode population densities. meloidogyne incognita and trichodorus christiei increased rapidly on corn and cotton, but were suppressed by peanut and soybean. more ...197519308149
body wall fine structure of the anterior region of meloidogyne incognita and heterodera glycines males.the body wall fine structure including the cuticle, hypodermis, and somatic muscles is similar in males of meloidogyne incognita and heterodera glycines. the cuticle can be regarded as basically three-layered in both species, but is much thicker in m. incognita than in h. glycines, and differences occur in surface markings. the chordal and interchordal hypodermis is syncytial. hypodermal tissue pervades the lip region, and lines the stomatal cavity and stylet shaft. various organelles and struct ...197519308152
effects of soil temperatures and inoculum levels of meloidogyne incognita and rhizoctonia solani on seedling disease of cotton.soreshin of cotton was more severe from combined infections of rhizoctonia solani and meloidogyne incognita than from either organism alone, when both critical soil temperature and inoculum concentrations were present. optimum soil temperatures for disease development from combined infections were 18 and 21 c. either 2,500 or 5,000 m. incognita larvae per plant, combined with r. solani, increased seedling disease severity over that caused by r. solani alone. when 100 or 500 larvae per plant were ...197519308161
effects of soil texture on the interaction between rhizoctonia solani and meloidogyne incognita on cotton seedlings.soils containing 60, 75, and 90% coarse particles (sand plus coarse silt) were prepared by dilution of a field soil with 246microm (60-mesh) silica sand. as the coarse-particle content of the soils increased, the synergistic interaction between meloidogyne incognita and rhizoctonia solani on cotton seedlings increased. increasing the coarse-particle content of the soil also increased damage from the nematode alone and slightly increased soreshin damage from r. solani alone.197519308162
meloidogyne incognita-induced changes in cell permeability of galled roots.electrolyte leakage of meloidogyne incognita-infected and healthy tomato roots was compared by conductivity measurements, and by compartmental analysis using rb. conductivity measurements suggested difference in electrolyte loss from healthy and galled roots. on a percentage basis, a greater rate of efflux occurred for healthy plants, but galled roots contain more electrolytes and may show a larger net loss. compartmental analysis indicated that: (i) the longer half-time for rb loss from vacuole ...197519308167
influence of meloidogyne incognita on the content of amino acids and nicotine in tobacco grown under gnotobiotic conditions.seedlings of meloidogyne incognita-resistant (n.c. 95) and -susceptible (mcnair 30) tobacco cultivars were grown aseptically for 55 days inside isolator chambers in autoclaved soil infested with 0 or 3,000 axenized eggs of m. incognita per 500 cc of soil. healthy and infected plants were compared. dry root weights of infected plants of resistant and susceptible cultivars were 16% and 84%, respectively, less than the controls. sixteen amino acids, including those precursors for nicotine, and nico ...197519308177
relationships between the population density of meloidogyne incognita and growth of tobacco.seedlings of tobacco cultivars resistant (nc95) and susceptible (mcnair 30) to meloidogyne incognita were grown in 15-cm diameter clay pots containing steamed soil infested with 0, l, 2, 4, 8, 16, 32, and 64 eggs of m. incognita per 1.5 cm(3) soil. plants were maintained in the greenhouse for 3 weeks, and then transferred to the field for 12 weeks. growth of tobacco was expressed separately as dry weight of leaves and as plant height. least squares regression analysis showed that tobacco growth- ...197519308181
effects of light intensity and quality effects on reproduction of plant-parasitic nematodes.growing cotton in a greenhouse with 12-h of supplemental light [8,608 lux (800 ft-c) from combination of mercury and lucalux lamps] resulted in 2 x to > 3 x greater reproduction of meloidogyne incognita and belonolaimus longicaudatus as compared to natural light alone. rate of increase of hoplolaimus galeatus was affected little in this experiment. in a second experiment under controlled conditions in a phytotron, light source and intensity had greater influence on the reproduction of heterodera ...197519308183
comparative fine structure of the stomatal region of males of meloidogyne incognita and heterodera glycines.the fine structure of the stylet, "guiding apparatus," anti protractor muscles of males of meloidogyne incognita and heterodera glycines is ehtcittated and compared. in both nematodes, the stylet cone is of greater electron density than the shaft. the cone is heterogeneous; shaft material extends into it for most of its length, whereas the shaft proper and knob regions are relatively homogeneous. the stylet lumnen is round throughout its length in h. glycines, but in m. incognita changes from ro ...197619308188
interactions of concomitant species of nematodes and fusarium oxysporum f. sp. vasinfectum on cotton.meloidogyne incognita, hoplolaintus galeatus, and north carolina and georgia populations of belonolaimus longicaudatus were introduced singly and in various combinations with fusarium oxysporum f. sp. vasinfectum on wilt-susceptible 'rowden' cotton. of all the nematodes, the combination of the n. c. population of b. longicaudatus with fusarium promoted greatest wilt development. h. galeatus had no effect on wilt. with fusarium plus m. incognito or b. longicaudatus, high nematode levels promoted ...197619308201
the influence of trichoderma harzianum on the root-knot fusarium wilt complex in cotton.wilt-susceptible cultivar 'rowden' cotton was inoculated wilh meloidogyne incognita (n), trichoderma harzianum (t), and fusarium oxysporum f. sp. vasinfectum (f) alone and in all combinations in various time sequences. plants inoculated with f alone or in combination with t did not develop wilt, simultaneous inoculation of 7-day-old seedlings with all three organisms (ntf) produced earliest wilt. however, plants receiving nematodes at 7 days and fusarium and trichoderma at 2 or 4 weeks later (n- ...197619308202
adenosine triphosphate quantification as related to cryptobiosis, nematode eggs, and larvae.sonification was the most effective method used for disintegrating nematode eggs and larvae for adenosine triphosphate (atp) determinations. sensitivity of the assay was sufficient to measure atp in one larva. second-stage larvae of anguina tritici averaged 1 x 10 femtograms (fg) atp and meloidogyne incognita eggs, 0.8 x 105 fg atp. larvae of panagrellus redivivus, a saprobe, averaged 12.2 x 105 fg atp, a measurement which was considerably higher than the atp levels in plant parasites. endophyti ...197619308214
relationships of initial population densities of meloidogyne incognita and m. hapla to yield of tomato.microplots 80 x 100 cm, infested with varying initial population densities (p(i)) of meloidogyne incognita or m. hapla, were planted to tomato at two locations. experiments were conducted in a sandy loam soil at fletcher, n. c. (mountains) where the mean temperature for may to september is ca 20.7 c, and in a loamy saml at clayton, n. c. (coastal plain) where the mean temperature for may to septemher is ca 24.8 c. in these experimentally infested plots, m. incognita and m. hapla caused maximum y ...197619308228
nature of sweet potato resistance to meloidogyne incognita and the effects of temperature on parasitism.penetration, rate of development, and total population of meloidogyne incognita in roots of susceptible 'allgold' and resistant 'nemagold' sweet potatoes increased with temperature 24-32 c. rate of larval penetration in 'allgold' was significantly higher than in 'nemagold' after 48 hr of root exposure at 24, 28, and 32 c. at 24, 28, and 32 c (16 hr) day and 20 c (8 hr) night temperature the life cycle of m. incognita required 42, 32, and 28 days in 'allgold', and 44, 33, and 31 days in 'nemagold ...197219319238
control of the root-knot nematode, meloidogyne incognita, on mimosa (albizzia julibrissin) by chemical dips. 197219319246
histopathology of thuja orientalis and juniperus horizontalis plumosa infected with meloidogyne incognita. 197219319248
killing and preserving nematodes in soil samples with chemicals and microwave energy.three basic procedures for treating nematode-bearing soil samples for international shipment or from areas under quarantine were tested for their killing effect and recovery of nematodes by sugar flotation for diagnostic and advisory purposes. these were: fumigation with methyl bromide followed by storage at -15 c; microwave treatment (2450 mhz, 630 w, 2-5 min) followed by addition of faa + picric acid or 5% formalin; and adding chemical preservatives (faa + picric acid, 5% formalin, nan, and 2- ...197219319249
serological relationship of meloidogyne incognita and m. arenaria.eight to ten precipitin bands were formed in a double immunodiffusion system comparing antigens of adult females of meloidogyne incognita and m. arenaria. most of the precipitin bands, based on band position and coalescence, were common to both species. antiserum specific for m. incognita was prepared by cross absorption. two populations of m. incognita were serologically identical, whereas two populations of m. arenaria differed slightly with respect to one weak precipitin band.197219319255
interactions of pratylenchus penetrans and meloidogyne incognita as coinhabitants in tomato.greenhouse experiments on the interactions of pratylenchus penetrans and meloidogyne incognita showed that the population densities of both nematode species were depressed whenthey coinhabited tomato roots. fifty days after inoculation, the population level of a p. penetrans monoculture was about four times higher than when m. incognita was present. conversely, m. incognita reproduced twice as fast alone as in combination. there were no significant differences in the numbers of p. penetrans when ...197219319262
disc-electrophoretic studies of soluble proteins and enzymes of meloidogyne incognita and m. arenaria.soluble-protein and eight enzyme profiles obtained by polyacrylamide-gel electrophoresis were compared between meloidogyne incognita and m. arenaria. esterase, malate dehydrogenase, and alpha-glycerophosphate dehydrogenase patterns were distinctly characteristic for each species. peroxidase and alpha-glycerophosphate dehydrogenase isoenzyme patterns varied when nematodes were propagated on different host plants. similar profiles were obtained for two populations within each species. antigenic pr ...197219319264
infection of seedlings of alfalfa and red clover by concomitant populations of meloidogyne incognita and pratylenchus penetrans.invasion of 2-day-old seedlings of 'buffalo' alfalfa and 'kenland' red clover by larvae of m. incognita and adults of p. penetrans, during 1-3 day periods of incubation at 24 c, was investigated in 50-mm petri dishes on 1% agar. penetration by both nematodes increased arithmetically with increased numbers in inocula. p. penetrans invaded alfalfa more readily than red cover, but m. incognita invaded red clover more readily than alfalfa. both nematodes inhibited root-elongation of alfalfa more tha ...197219319281
relative efficacy of selected volatile and nonvolatile nematicides for control of meloidogyne incognita on tobacco.root-knot nematode control and tobacco yields in plots infested with meloidogyne incognita and treated with the nonvolatile nematicides, aldicarb, mocap (r), or nemacur (r) were greater than those on similar plots treated with volatile nematicides such as dd, dd + mencs, sd14647 or tetrachlorothiophene. root-knot control and tobacco yields in plots treated with carbofuran or dasanit (r) were eqtual to that obtained with dd + mencs, but less than that obtained with the other volatile soil nematic ...197319319289
nematode response to carbofuran.higher populations of meloidogyne incognita larvae and pratylenchus penetrans were recovered from soil treated with carbofuran 10 and 15 days after treatment, respectively, than were recovered from untreated control soil. the number of p. penetrans, however, was lower 50 days after treatment, and symptoms developed only occasionally on the root systems of host plants. populations of tylenchorhynchus claytoni inoculated at different distances from the base of corn seedlings growing in carbofuran- ...197319319291
variation in pathogenicity of seventeen isolates of meloidogyne incognita.pathogenicity of 17 isolates of meloidogyne incognita collected in tennessee was studied in the greenhouse on: rutgers tomato, n.c. 95 tobacco, mcnair 1032 cotton, dixie queen watermelon, california wonder pepper and line m57-13n cowpea. root-knot indices of the isolates on the different hosts differentiated six physiological races. the host reactions of each race are discussed.197319319300
population dynamics of plant nematodes in cultivated soil: length of rotation in newly cleared and old agricultural land.during a 6-year study of 1-, 2-, and 3-year crop rotations, population densities of pratylenchus brachyurus, trichodorus christiei, and meloidogyne incognita were significantly affected by the choice of crops but not by length of crop rotation. the density of p. brachyurus and t. christiei increased rapidly on milo (sorghum vulgate). in addition, populations of p. brachyurus increased significantly in cropping systems that involved crotalaria (c. rnucronata), millet (setaria italica), and sudang ...197319319315
hatching of meloidogyne incognita eggs in the neutral carbohydrate fraction of root exudates of gnotobiotically grown alfalfa.meloidogyne incognita eggs were hatched in soil sterilized by gamma kradiation and wetted with root exudates from alfalfa plants in different stages of development and subjected to various levels of clipping. carbohydrate components of the exudates were identified by gas chromatography-mass spectrometry. although significant stimulation of hatch was detected in exudates of seedling and flowering plants, the practical importance of the increase is doubtful as hatch in distilled water was always g ...197319319320
influence of concomitant pratylenchus brachyurus and meloidogyne spp. on root penetration and population dynamics.populations of pratylenchus brachyurus on cotton were increased significantly in the presence of either meloidogyne incognita or m. arenaria.this occurred with either simultaneous inoculation or prior invasion by m. incognita. p. brachyurus penetrated cotton roots previously invaded by, or simultaneously inoculated with, m. incognita, as well as, or better than, in the absence of m. incognita. prior invasion by m. incognita, however, suppressed p. brachyurus populations on tomato, while it had n ...197319319334
phenol accumulation related to resistance in tomato to infection by root-knot and lesion nematodes.host-parasite relationships of meloidogyne incognita acrita and pratylenchus penetrans were compared on three closely related cultivars of tomato: 'nemared', resistant to root-knot nematodes; 'hawaii 7153', moderately resistant; and 'b-5', susceptible. root-knot nematode larvae induced typical galls on the roots of b-5; larvae that entered nemared were walled-off by necrotic cells; both reactions occurred in hawaii 7153. lesion nematodes caused surface lesions which were initially similar on all ...197319319346
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