Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
field survey focused on opisthorchis viverrini infection in five provinces of cambodia.opisthorchiasis is endemic in thailand and lao people's democratic republic and constitutes a major public health problem throughout the mekong basin. although cambodia is located in the mekong basin, the status of o. viverrini infection in that country was not previously clarified. this research was conducted to document the extent and distribution of o. viverrini infection in cambodia.201424342554
endemicity of opisthorchis viverrini liver flukes, vietnam, 2011-2012. 201424377371
association between praziquantel and cholangiocarcinoma in patients infected with opisthorchis viverrini: a systematic review and meta-analysis.the liver fluke, opisthorchis viverrini, and the associated incidence of subsequent cholangiocarcinoma (cca) are still a public health problem in thailand, and praziquantel (pzq) remains the antihelminthic drug of choice for treatment. evidence in hamsters shows that repeated infection and pzq treatments could increase the risk of cca. however, the existing evidence in humans is inconclusive regarding increased risk of cca with frequency of pzq intake.201324377641
dna damage in inflammation-related carcinogenesis and cancer stem cells.infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells and result in oxidative and nitrative dna damage, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in the initiation and/or promotion of inflammation-mediated carcinogen ...201324382987
retrotransposon ov-rte-1 from the carcinogenic liver fluke opisthorchis viverrini: potential target for dna-based diagnosis.infections by the fish-borne liver flukes opisthorchis viverrini and clonorchis sinensis can lead to bile duct cancer. these neglected tropical disease pathogens occur in east asia, with o. viverrini primarily in thailand and laos and c. sinensis in cambodia, vietnam, and china. genomic information about these pathogens holds the potential to improve disease treatment and control. transcriptome analysis indicates that mobile genetic elements are active in o. viverrini, including a novel non-long ...201424394447
roles of liver fluke infection as risk factor for cholangiocarcinoma.several factors are known to be associated with risk of cholangiocarcinoma (cca) and infection with the liver flukes, opisthorchis viverrini and clonorchis sinensis, has often been singled out as the leading risk factor in east and southeast asia. in this review, current knowledge of their biology, life cycle, and pathogenesis of o. viverrini, and its role as a carcinogenic parasite are presented. the trends of age-specific incidence of liver cancer in khon kaen, northeast thailand are considere ...201424408775
risk biomarkers for assessment and chemoprevention of liver fluke-associated cholangiocarcinoma.human liver fluke, opisthorchis viverrini (ov), is the major risk factor of cholangiocarcinoma (cca) in northeastern thailand. our approach focuses on genetic progression and molecular changes in the carcinogenic pathway of liver fluke-associated cca aimed at assessing patients at risk of cca and using chemoprevention as the secondary cancer prevention to reduce the incidence of cca. this review summarizes altered gene expressions, biomolecules and their modification, i.e. dna adducts, oxidized ...201424408859
potential targeted therapy for liver fluke associated cholangiocarcinoma.biliary tree cancer or cholangiocarcinoma (cca) is an unusual subtype of liver cancer with exceptionally poor prognosis. lack of specific symptoms and availability of early diagnostic markers account for late diagnosis of cca. surgical treatment is a gold standard choice but few patients are candidates and local recurrence after surgery is high. benefit of systemic chemotherapy is limited; hence, better treatment options are required. the differences in etiology, anatomical positions and patholo ...201424408866
histological confirmation of periductal fibrosis from ultrasound diagnosis in cholangiocarcinoma patients.cholangiocarcinoma (cca) has no specific clinical signs and symptoms and non-specific bio- and tumor-markers in the early disease stage. usually patients present to tertiary care with advanced disease stage. in order to detect early cases of cca that may present as a mass, dilatation of intrahepatic duct or combination, ultrasonography is accepted as a powerful imaging tool. a smaller mass or bile duct segmental dilatation requires further imaging for characterization, including computerized tom ...201424420706
analysis of new biomarkers for cholangiocarcinoma.cholangiocarcinoma is one of the most serious diseases in northeast thailand, where its incidence is reported to be the highest in the world. we tried to develop a new method to detect cholangiocarcinoma in the early stages using serum proteins. we found that after fluorescent labeling of the sugar moiety of serum proteins, a new peak was identified, which might be a promising marker for cholangiocarcinoma.201424446393
helminth infection in southern laos: high prevalence and low awareness.helminthiasis is a public health concern in lao people's democratic republic (lao pdr, laos). this study aimed to understand helminth infection and associated risk factors in relation to existing local knowledge, attitudes and practices regarding worm infections in endemic communities.201324499584
prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand.to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region.201424507641
molecular differentiation of opisthorchis viverrini and clonorchis sinensis eggs by multiplex real-time pcr with high resolution melting analysis.opisthorchis viverrini and clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in asia. the standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. however, the method has low sensitivity, and eggs of o. viverrini and c. sinensis are difficult to distinguish from each other and from those of some other trematodes. here, we report a multiplex real-time pcr coupled with high resolution melting (hrm) ...201324516275
curcumin prevents bile canalicular alterations in the liver of hamsters infected with opisthorchis viverrini.opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. little is known about the pathological alterations in bile canaliculi in opisthorchiasis. this study aimed to investigate bile canalicular alterations in o. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. hamsters were infected with o. viverrini and one group of animals was fed with 1% dietary curcumin supplement. animals were examined during t ...201324516276
distribution and abundance of opisthorchis viverrini metacercariae in cyprinid fish in northeastern thailand.to increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of opisthorchis viverrini metacercariae (ov mc) was investigated in freshwater fish obtained from 20 provinces in northeastern thailand between april 2011 and february 2012. a cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. fish, were collected in each of the rainy and w ...201324516277
establishment of an allo-transplantable hamster cholangiocarcinoma cell line and its application for in vivo screening of anti-cancer drugs.opisthorchis viverrini (o. viverrini) is a well-known causative agent of cholangiocarcinoma (cca) in humans. cca is very resistant to chemotherapy and is frequently fatal. to understand the pathogenesis of cca in humans, a rodent model was developed. however, the development of cca in rodents is time-consuming and the xenograft-transplantation model of human cca in immunodeficient mice is costly. therefore, the establishment of an in vivo screening model for o. viverrini-associated cca treatment ...201324516278
a cross-sectional study on intestinal parasitic infections in rural communities, northeast thailand.despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in southeast asia, including thailand. in rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. this study presents a survey of the prevalence of intestinal parasitic infections among the people in rural thailand. the community-based cross-sectional study was conducted in villages in khon ...201324516280
high prevalence of haplorchiasis in nan and lampang provinces, thailand, proven by adult worm recovery from suspected opisthorchiasis cases.opisthorchiasis, a risk factor for cholangiocarcinoma in humans, is of public health importance in thailand. the annual surveillance reports from nan and lampang provinces, thailand, for the year 2011 showed an opisthorchiasis prevalence of over 70% by recovery of eggs in the feces. this study investigated whether most cases are actually due to minute intestinal flukes (mif) rather than opisthorchis viverrini, as the eggs of both can hardly be differentiated by morphology. fifty and 100 cases fr ...201324516289
genomic and transcriptional alterations of cholangiocarcinoma.cholangiocarcinoma (cca) is one of the representative cancers refractory to any therapeutic approach. the incidence of cca is highest in the northeastern part of thailand, where chronic inflammation caused by liver fluke (opisthorchis viverrini: ov) infection is a major etiologic factor. the incidence of cca is also increasing in other countries, including japan. here, we overview the genetic and transcriptional alterations of cca with and without association with ov infection. cca with ov shows ...201424532422
an ecological study of bithynia snails, the first intermediate host of opisthorchis viverrini in northeast thailand.infection with the food-borne trematodiasis, liver fluke opisthorchis viverrini, is a major public health concern in southeast asia. while epidemiology and parasitic incidence in humans are well studied, ecological information on the o. viverrini intermediate hosts remains limited. this study aimed to investigate the factors affecting the distribution and abundance of the first intermediate host, bithynia siamensis goniomphalos snails. water quality and snails were sampled in 31 sites in muang d ...201524561073
development of a pcr assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens.small liver and minute intestinal flukes are highly prevalent in southeast asia. definitive diagnosis of parasite infection is usually achieved parasitologically by finding the fluke eggs in feces. however, their eggs are difficult to differentiate morphologically in fecal samples, even for experienced technicians. the present study developed a pcr assay coupled with dna pyrosequencing for identification of the fish-borne trematodes (fbt), opisthorchis viverrini, clonorchis sinensis, haplorchis ...201424589167
cytokine/chemokine secretion and proteomic identification of upregulated annexin a1 from peripheral blood mononuclear cells cocultured with the liver fluke opisthorchis viverrini.we investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (pbmcs) cocultured with adult liver flukes (opisthorchis viverrini) for 6 to 24 h. pbmc-derived proteins were identified by two-dimensional electrophoresis and mass spectrometry, and the cytokines/chemokines in the supernatant were assessed using a cytokine array. exposure to o. viverrini induced increases in secretion of proinflammatory cytokines, costimulating protein, ...201424614660
zoonotic trematode metacercariae in fish from phnom penh and pursat, cambodia.a survey was performed to investigate the infection status of freshwater fish with zoonotic trematode metacercariae in phnom penh and pursat province, cambodia. all collected fish with ice were transferred to our laboratory and examined using the artificial digestion method. in fish from phnom penh, 2 kinds of metacercariae (opisthorchis viverrini and haplorchis yokogawai) were detected. o. viverrini metacercariae were positive in 37 (50.0%) of 74 fish in 11 species (average no. metacercariae/fi ...201424623879
prevalence of helminthic infections among inhabitants of lao pdr.the prevalence of helminthic infections was surveyed on a total of 6,178 residents (males 2,549 and females 3,629) in 102 villages of 9 provinces in lao pdr between 2007 and 2011 under the project of korea-laos collaborative project for control of foodborne trematode infections in lao pdr. fecal specimens were collected and examined by the kato-katz thick smear and stoll's egg counting techniques. the overall liver/intestinal helminth egg positive rate was 71.9% with a single or mixed infections ...201424623882
intestinal parasitic infections in hiv-infected patients, lao people's democratic republic.hiv infection is an emerging problem in laos. we conducted the first prospective study on intestinal parasites, including opportunistic protozoa, in newly diagnosed hiv infected patients, with or without diarrhea. the aims were to describe the spectrum of infections, to determine their prevalence and to assess their associations with diarrhea, cd4 cell count, place of residence and living conditions.201424662743
rna-seq reveals infection-induced gene expression changes in the snail intermediate host of the carcinogenic liver fluke, opisthorchis viverrini.bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, opisthorchis viverrini, the leading cause of cholangiocarcinoma (cca) in the greater mekong sub-region of thailand. despite the severe public health impact of opisthorchis-induced cca, knowledge of the molecular interactions occurring between the parasite and its snail intermediate host is scant. the examination of differences in gene expression profiling between uninfected and o. viverrini-infected b. siamensis g ...201424676090
perceived illness drives participation in mass deworming campaigns in laos.multiple helminth infections are very common in communities of southern laos. preventive chemotherapy in combination with health education is the mainstay of control. we assessed the knowledge, perceptions and practices of rural communities related to endemic helminthiasis and their control during a mass drug administration (mda) campaign. short interviews with household heads (n=192), direct observations and discussions with attendees of mdas, and in-depth interviews with local opinion leaders ...201524713201
patterns of trematode infections of opisthorchis viverrini (opisthorchiidae) and haplorchis taichui (heterophyidae) in human populations from two villages in savannakhet province, lao pdr.the liver fluke, opisthorchis viverrini, and the minute intestinal fluke, haplorchis taichui, are prevalent in many asian countries. this study analysed the patterns of infections of o. viverrini and h. taichui in lahanam and thakhamlien villages (savannakhet province, lao pdr), in two cross-sectional investigations. out of a total of 207 human participants, post-anthelmintic treatment positivity rates for expelled worms were 170 (82.1%) for h. taichui and 65 (31.4%) for o. viverrini. both these ...201524739959
prevalence of haplorchis taichui among humans and fish in luang prabang province, lao pdr.this study confirmed the prevalence of the intestinal fluke haplorchis taichui (trematoda: heterophyidae) among people and fish in luang prabang province, lao pdr. fecal specimens were collected from 559 riparian people (229 males and 330 females), residing in 4 districts (luang prabang, xieng ngeun, pak ou, and nam bak) and were examined by the kato-katz fecal smear technique. the overall helminth egg positive rate was 64.9%. the positive rate for small trematode eggs (ste), which may include h ...201424754919
experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus.opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24mm) of barbonymus gonionotus fish on infection success of o. viver ...201424780189
effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review.melatonin, a hormone secreted by the pineal gland, works directly and indirectly as a free radical scavenger. its other physiological or pharmacological activities could be dependent or independent of receptors located in different cells, organs, and tissues. in addition to its role in promoting sleep and circadian rhythms regulation, it has important immunomodulatory, antioxidant, and neuroprotective effects suggesting that this indole must be considered as a therapeutic alternative against inf ...201424811367
suppression of aquaporin, a mediator of water channel control in the carcinogenic liver fluke, opisthorchis viverrini.opisthorchiasis and opisthorchis viverrini-associated bile duct cancer represent major public health threats in thailand and laos. the tegument of this food borne fluke plays pivotal roles in parasite metabolism, homeostasis and osmoregulation. excretory/secretory products also pass from the fluke to the biliary environment, products that likely underlie pathogenesis of liver fluke infection. aquaporins (aqps), belong to the major intrinsic protein superfamily of integral plasma membrane channel ...201424885060
hepatitis b virus infection and intrahepatic cholangiocarcinoma.intrahepatic cholangiocarcinoma (icc) is a devastating malignant tumor arising from the peripheral intrahepatic bile duct epithelium. the incidence and mortality of icc is markedly increasing over the past two decades worldwide, though the cause for this rise in incidence is unclear, thus intensifying the search for alternative etiological agents and pathogenetic mechanisms. hepatolithiasis, primary sclerosing cholangitis, parasitic infection (opisthorchis viverrini or clonorchis sinensis), fibr ...201424914333
first insight into cd59-like molecules of adult fasciola hepatica.the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ...201424955521
localization of the cystogenous glands of opisthorchis viverrini cercariae.opisthorchiasis in northeastern thailand is an important etiology of cholangiocarcinoma. to form the infectious stage, free swimming cercariae penetrate cyprinid fish, shed their tails, and then secret a cystic substance to cover their larval stage to form metacercariae in the fish body. we determined the location of the cystogenous glands in opisthorchis viverrini cercariae. the cercariae and metacercariae were obtained from the naturally infected snail host, bithynia siamensis goniomphalos and ...201424968667
risk factors for opisthorchis viverrini and minute intestinal fluke infections in lao pdr, 2009-2011.the purpose of this report is to compare the prevalence of opisthorchis viverrini/minute intestinal fluke (ov/mif) in lao people's democratic republic according to different regions and evaluate the effect of ingestion of raw freshwater fish on the infection. demographic data, behavioral data, and symptoms were obtained, and the kato-katz method was used for the diagnosis of ov/mif. in total, 3,046 individuals were enrolled. the risk factors associated with acquisition of ov/mif were raw fish co ...201424980495
the opisthorchis viverrini genome provides insights into life in the bile duct.opisthorchiasis is a neglected, tropical disease caused by the carcinogenic asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is linked to malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia. no vaccine is available, and only one drug (praziquantel) is used against the parasite. little is known about o. viverrini biology and the diseases that it causes. here we characterize the draft genome (634.5 mb) and transcriptomes of o. viverrini, elucidate ...201425007141
distinct mirna signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke opisthorchis viverrini.intrahepatic cholangiocarcinoma (icc) is a significant public health problem in east asia, where it is strongly associated with chronic infection by the food-borne parasite opisthorchis viverrini (ov). we report the first comprehensive mirna expression profiling by microarray of the most common histologic grades and subtypes of icc: well differentiated, moderately differentiated, and papillary icc.201425017828
analysis of the population genetics of opisthorchis viverrini sensu lato in the nam ngum river wetland, lao pdr, by multilocus enzyme electrophoresis.a previous population genetics study of opisthorchis viverrini from a locality in an endemic area in thailand found little genetic variation over time and second intermediate fish host species. since a similar comparative analysis is not available for lao pdr, we conducted a study of o. viverrini from different endemic foci in vientiane province, lao pdr, based on spatial, temporal and fish host species. a total of 620 adult o. viverrini originating from the nam ngum river wetland were analysed ...201425033811
an interleukin-6 receptor polymorphism is associated with opisthorchiasis-linked cholangiocarcinoma risk in thailand.the cholangiocarcinoma (cca) is a relatively rare cancer worldwide but it is highly prevalent in thailand where the liver fluke, opisthorchis viverrini is endemic. there are reports that interleukin 6 (il-6) may play an important role in the pathogenesis of opisthorchiasis associated cca. functionally, il-6 can act on target cells through its receptor, il-6r, and il-6r polymorphisms may affect the functional activity of il-6 leading to susceptibility to cholangiocarcinogenesis. therefore, we ass ...201425041016
stats profiling reveals predominantly-activated stat3 in cholangiocarcinoma genesis and progression.we investigated the aberrant expression of the stat family in humans and liver fluke (opisthorchis viverrini, ov)-induced hamster cholangiocarcinoma (cca) tissues.201425044480
toward integrated opisthorchiasis control in northeast thailand: the lawa project.human liver fluke, opisthorchis viverrini, a food-borne trematode is a significant public health problem in southeast asia, particularly in thailand. despite a long history of control programmes in thailand and a nationwide reduction, o. viverrini infection prevalence remains high in the northeastern provinces. therefore, a new strategy for controlling the liver fluke infection using the ecohealth/one health approach was introduced into the lawa lake area in khon kaen province where the liver fl ...201525102053
opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like.concurrent deficiency of rhophilin associated tail protein (ropn1) and ropn1-like (ropn1l) in mice causes structural abnormalities and immotility of sperm and thereby infertility. in the present research, ropn1l of the human liver fluke opisthorchis viverrini was molecularly characterized and showed unexpected potential as a diagnostic tool. ropn1l transcripts were detected in 2-week-old juveniles by rt-pcr. immunohistochemical analysis of the adult worm localized the protein in testis lobes, se ...201425124046
circulating mir-192 in liver fluke-associated cholangiocarcinoma patients: a prospective prognostic indicator.this study aimed to investigate the mir-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (cca) for a prospective prognostic indicator.201425131257
prevalence and risk factors for opisthorchis viverrini infections in upper northeast thailand.opisthorchis viverrini is an ongoing public health problem in northeast thailand. despite continuous efforts for decades by healthcare organizations to overcome this problem, infection rates remain high. to enable related personnel to identify and address the various issues effectively, a cross-sectional study was performed to investigate prevalence and risk factors for opisthorchiasis. the target group was 3,916 thai residents of northeast thailand who were 15 or over. participants were recruit ...201425169496
repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a cross-sectional survey in southern lao people's democratic republic.intestinal parasitic infections are common in lao people's democratic republic (lao pdr). we investigated the accuracy of the kato-katz (kk) technique in relation to varying stool sampling efforts, and determined the effect of the concurrent use of a quantitative formalin-ethyl acetate concentration technique (fect) for helminth diagnosis and appraisal of concomitant infections. the study was carried out between march and may 2006 in champasack province, southern lao pdr. overall, 485 individual ...201525225157
specific diagnosis of opisthorchis viverrini using loop-mediated isothermal amplification (lamp) targeting parasite microsatellites.opisthorchis viverrini and other food-borne trematode infections are major health problems in thailand, the lao people's democratic republic, vietnam and cambodia. differential diagnosis of o. viverrini based on the microscopic observation of parasite eggs is difficult in areas where clonorchis sinensis and minute intestinal flukes coexist. recently, loop-mediated isothermal amplification (lamp) has been widely used for detection and identification of trematode for its simple method that is usef ...201525268466
subtle to severe hepatobiliary morbidity in opisthorchis viverrini endemic settings in southern laos.evidence of severe hepatobiliary morbidity associated with opisthorchis viverrini liver fluke infection including cholangiocarcinoma (cca) is scarce in laos although o. viverrini infection is highly prevalent. we assessed hepatobiliary morbidity using abdominal ultrasonography (us) in o. viverrini adult patients in saravan province, southern laos. a random sample of 431 o. viverrini patients from 10 villages underwent abdominal us. mild, moderate and markedly advanced periductal fibrosis was dia ...201525275346
proteomic profile of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini.the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. the undisputed link between cca and o. viverrini infection has precipitated efforts to understand the molecular basis of host-parasite interactions with a view to ultimately developing new control strategies to combat this carcinogenic infection. to date most effort has focused on the interactions betwee ...201525284051
epidemiology of strongyloides stercoralis on mekong islands in southern laos.strongyloides stercoralis is a neglected helminth infection potentially that can lead to systemic infection in immunocompromised individuals. in lao people's democratic republic (lao pdr, laos), information on s. stercoralis infection is scarce. we assessed s. stercoralis infection and associated risk factors and symptoms on the mekong islands in southern laos. baermann and kato-katz techniques were performed on two stool samples from each individual to detect s. stercoralis larvae and concomita ...201525291044
multiparasitism and intensity of helminth infections in relation to symptoms and nutritional status among children: a cross-sectional study in southern lao people's democratic republic.the occurrence and spatial distribution of intestinal helminth infection in children is fairly well understood. however, knowledge on how helminth infections govern intestinal morbidity is scarce. we conducted a cross-sectional study to assess and quantify the relationship between single and multiple species helminth infection with clinical and self-reported morbidity indicators and nutritional status in champasack province, southern lao people's democratic republic (lao pdr). a random sample of ...201525291046
immunization and challenge shown by hamsters infected with opisthorchis viverrini following exposure to gamma-irradiated metacercariae of this carcinogenic liver fluke.here we report findings to optimize and standardize conditions to attenuate metacercariae of opisthorchis viverrini by ionizing radiation to elicit protective immune responses to challenge infection. metacercariae were gamma-irradiated and the ability of irradiated metacercariae to prevent patent infection of challenge metacercariae in hamsters was determined, as well as their ability to induce a host antibody response. metacercariae irradiated in a dose-dependent manner, with 3, 5, 10, 12, 20, ...201625315797
gene-environment interaction involved in cholangiocarcinoma in the thai population: polymorphisms of dna repair genes, smoking and use of alcohol.cholangiocarcinoma (cca) is the most common malignancy in a northeast thai population. smoking and alcohol drinking are associated with the production of free radical intermediates, which can cause several types of dna lesions. reduced repair of these dna lesions would constitute an important risk factor for cancer development. we therefore examined whether polymorphisms in dna base-excision repair (ber) genes, xrcc1 g399a and ogg1 c326g, were associated with cca risk and whether they modified t ...201425335960
rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini using real-time pcr and high resolution melting analysis.clonorchis sinensis and opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in asia. the present study developed an assay integrating real-time pcr and high resolution melting (hrm) analysis for the specific detection and rapid identification of c. sinensis and o. viverrini. primers targeting cox1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. assays using genomic dna ex ...201425386621
genetic and environmental determinants of risk for cholangiocarcinoma in thailand.cholangiocarcinoma (cca) is a difficult cancer to diagnose in the early stage and to treat by curative resection. the incidence of cca in the northeast of thailand is the highest in the world. to make progress in detecting a high risk group and in the prevention and detection of cca, we have been analyzing the risk factors for cca. although liver fluke infection is known to be a risk factor, there are patients who are not infected with the liver fluke and not all people infected with the liver f ...201425401000
selective immunostaining of mehlis' gland of opisthorchis viverrini by antibody against rat diacylglycerol kinase.the mehlis' gland of opisthorchis viverrini was selectively and intensely immunopositive with an antibody against rat diacylglycerol kinase gamma, and its entire structure with associated radiating processes was clearly demonstrated by immuno-light microscopy. in immuno-electron microscopy, the immunopositive processes were revealed to contain many vesicles and vacuoles and the immunoreactive materials were deposited diffusely in the cytoplasm except for the vesicular interior. the present findi ...201425417499
traditional herbal medicine for the control of tropical diseases.throughout history, traditional herbal medicine has afforded a rich repository of remedies with diverse chemical structures and bioactivities against several health disorders. a common issue of herbal medicine is the limitation of information on their pharmacological activities and their active constituents. traditionally, the use of herbal medicine has been based on empirical treatment and passed on from generation to generation with information available only in local journals. this prevents s ...201425425945
seasonal cercarial emergence patterns of opisthorchis viverrini infecting bithynia siamensis goniomphalos from vientiane province, lao pdr.snail intermediate hosts play a pivotal role in maintaining the life cycles of trematodes, including opisthorchis viverrini. we investigated the emergence patterns of o. viverrini cercariae infecting bithynia siamensis goniomphalos at foci in an endemic area in vientiane province, lao pdr.201425442515
reprint of "experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus".opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24 mm) of barbonymus gonionotus fish on infection success of o. vive ...201525446173
anti-inflammatory and anti-periductal fibrosis effects of an anthocyanin complex in opisthorchis viverrini-infected hamsters.the pharmacological activities of herbal extracts can be enhanced by complex formation. in this study, we manipulated cyanidin and delphinidin-rich extracts to form an anthocyanin complex (ac) with turmeric and evaluated activity against inflammation and periductal fibrosis in opisthorchis viverrini-infected hamsters. the ac was prepared from anthocyanins extracted from cobs of purple waxy corn (70%), petals of blue butterfly pea (20%) and turmeric extract (10%), resulting in an enhanced free-ra ...201425447758
suppression of ov-grn-1 encoding granulin of opisthorchis viverrini inhibits proliferation of biliary epithelial cells.multistep processes likely underlie cholangiocarcinogenesis induced by chronic infection with the fish-borne liver fluke, opisthorchis viverrini. one process appears to be cellular proliferation of the host bile duct epithelia driven by excretory-secretory (es) products of this pathogen. specifically, the secreted growth factor ov-grn-1, a liver fluke granulin, is a prominent component of es and a known driver of hyper-proliferation of cultured human and mouse cells in vitro. we show potent hype ...201525450776
bi-functionality of opisthorchis viverrini aquaporins.aquaporins (aqp) are essential mediators of water regulation in all living organisms and members of the major intrinsic protein (mip) superfamily of integral membrane proteins. they are potential vehicles or targets for chemotherapy, e.g. in trypanosoma brucei melarsoprol and pentamidine uptake is facilitated by tbaqp-2. transcriptome data suggests that there are at least three active aquaporins in the human liver fluke, opisthorchis viverrini, ovaqp-1, 2 and 3, and crude rna silencing of ovaqp- ...201525461277
infection and cancer: global distribution and burden of diseases.infection is one of the main risk factors for cancer.201425512154
prevalence of intestinal helminths among inhabitants of cambodia (2006-2011).in order to investigate the status of intestinal helminthic infections in cambodia, epidemiological surveys were carried out on a national scale, including 19 provinces. a total of 32,201 fecal samples were collected from schoolchildren and adults between 2006 and 2011 and examined once by the kato-katz thick smear technique. the overall egg positive rate of intestinal helminths was 26.2%. the prevalence of hookworms was the highest (9.6%), followed by that of opisthorchis viverrini/minute intes ...201425548418
chronic opisthorchis viverrini infection and associated hepatobiliary disease is associated with iron loaded m2-like macrophages.chronic opisthorchis viverrini-induced hepatobiliary disease is associated with significant leukocyte infiltration, including activated macrophages; however, the polarization of infiltrating macrophages remains to be fully characterized. in this study, we characterized macrophage polarization and phenotype in chronic o. viverrini-induced hepatobiliary disease in humans and hamsters using gene expression and histochemical analysis. chronic o. viverrini infection and associated hepatobiliary disea ...201425548425
[the reagents kit to detect metyorchis biis, opisthorchis viverrini and clonorchis sinensis, opisthorchis felineus--agents of opisthorchiasis using technique of polymerase chain reaction in real-time].the helminths opisthorchis felineus, opisthorchis viverrini, clonorchis sinensis, metorchis bilis are the agents of opisthorchiasis. the actual diagnostic of parasitic diseases based on microscope analysis of samples of human feces to detect presence of ova of parasites suffers of many shortcomings, in particular low sensitivity especially at earlier stages. the purpose of this study was to compare results of detection of parasites using both classical technique and technique of specific differe ...201425552048
lc-ms/ms method for the determination of two metabolites of tribendimidine, deacylated amidantel and its acetylated metabolite in plasma, blood and dried blood spots.tribendimidine has emerged as potential alternative to praziquantel in the treatment of opisthorchis viverrini infections. to support its clinical development program, a quantitative high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) assay was developed for tribendimidine's degradation product deacylated amidantel (dadt) and its acetylated metabolite adadt. analytical sample preparation included protein precipitation for blood and plasma, and direct processing of dried ...201525553533
opisthorchis viverrini infection activates the pi3k/ akt/pten and wnt/β-catenin signaling pathways in a cholangiocarcinogenesis model.opisthorchis viverrini (ov) infection is the major etiological factor for cholangiocarcinoma (cca), especially in northeast thailand. we have previously reported significant involvement of pi3k/akt/pten and wnt/β- catenin in human cca tissues. the present study, therefore, examined the expression and activation of pi3k/ akt/pten and wnt/β-catenin signaling components during ov-induced cholangiocarcinogenesis in a hamster animal model. hamsters were divided into two groups; non-treated and ov plu ...201425556493
genome mining offers a new starting point for parasitology research.parasites including helminthes, protozoa, and medical arthropod vectors are a major cause of global infectious diseases, affecting one-sixth of the world's population, which are responsible for enormous levels of morbidity and mortality important and remain impediments to economic development especially in tropical countries. prevalent drug resistance, lack of highly effective and practical vaccines, as well as specific and sensitive diagnostic markers are proving to be challenging problems in p ...201525563615
schistosome and liver fluke derived catechol-estrogens and helminth associated cancers.infection with helminth parasites remains a persistent public health problem in developing countries. three of these pathogens, the liver flukes clonorchis sinensis, opisthorchis viverrini and the blood fluke schistosoma haematobium, are of particular concern due to their classification as group 1 carcinogens: infection with these worms is carcinogenic. using liquid chromatography-mass spectrometry (lc-ms/ms) approaches, we identified steroid hormone like (e.g., oxysterol-like, catechol estrogen ...201425566326
bile canalicular changes and defective bile secretion in opisthorchis viverrini-infected hamsters.infection with the liver fluke opisthorchis viverrini (digenea) (poirier, 1886) causes bile duct injury and periductal fibrosis by chronic overproduction of inflammatory-mediators and eventually results in cholangiocarcinoma development. while extensive research works have been done on o. viverrini infection-associated changes of bile ducts and periductal fibrosis, little attention was paid on morphological and biochemical changes of the bile canaliculi (bc), the origin of bile flow. we aimed to ...201425651692
genetics of opisthorchis viverrini-related cholangiocarcinoma.we review the genetic, epigenetic and transcriptional landscape of liver fluke (opisthorchis viverrini, ov)-related cholangiocarcinoma (cca). its distinct alterations, as compared with non-ov-related cca may help shed light on its underlying molecular mechanisms.201525693006
protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l.fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ...201525701317
plasma phosphoproteome and differential plasma phosphoproteins with opisthorchis viverrini-related cholangiocarcinoma.this study was conducted to investigate the plasma phosphoproteome and differential plasma phosphoproteins in cases of of opisthorchis viverrini (ov)-related cholangiocarcinoma (cca). plasma phosphoproteomes from cca patients (10) and non-cca subjects (5 each for healthy subjects and ov infection) were investigated using gel-based and solution-based lc-ms/ms. phosphoproteins in plasma samples were enriched and analyzed by lc-ms/ms. strap, panther, ipath, and mev programs were applied for the ide ...201525735322
the carcinogenic liver fluke opisthorchis viverrini is a reservoir for species of helicobacter.there has been a strong, positive correlation between opisthorchiasis-associated cholangiocarcinoma and infection with helicobacter. here a rodent model of human infection with opisthorchis viverrini was utilized to further investigate relationships of apparent co-infections with o. viverrini and h. pylori. a total of 150 hamsters were assigned to five groups: i) control hamsters not infected with o. viverrini; ii) o. viverrini-infected hamsters; iii) non-o. viverrini infected hamsters treated w ...201525773821
genome-wide characterization of microsatellites and marker development in the carcinogenic liver fluke clonorchis sinensis.clonorchis sinensis is an important carcinogenic human liver fluke endemic in east and southeast asia. there are several conventional molecular markers that have been used for identification and genetic diversity; however, no information about microsatellites of this liver fluke is published so far. we here report microsatellite characterization and marker development for a genetic diversity study in c. sinensis, using a genome-wide bioinformatics approach. based on our search criteria, a total ...201525782682
plasma igg autoantibody against actin-related protein 3 in liver fluke opisthorchis viverrini infection.opisthorchiasis secondary to opisthorchis viverrini infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. it was hypothesized that plasma autoantibodies directed against self-proteins are biomarkers for opisthorchiasis. plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to lc-ms/ms. seven protein ...201525809205
gis-based spatial statistical analysis of risk areas for liver flukes in surin province of thailand.it is urgently necessary to be aware of the distribution and risk areas of liver fluke, opisthorchis viverrini, for proper allocation of prevention and control measures. this study aimed to investigate the human behavior, and environmental factors influencing the distribution in surin province of thailand, and to build a model using stepwise multiple regression analysis with a geographic information system (gis) on environment and climate data. the relationship between the human behavior, attitu ...201525824758
anthelmintic, anti-inflammatory and antioxidant effects of garcinia mangostana extract in hamster opisthorchiasis.administration of praziquantel for treatment of liver fluke infection may affect the host, with mild and severe effects after treatment caused by host immune response. therefore, we focused on the antioxidant property, inflammatory and anthelmintic effects of the traditional folk medicine, g. mangostana pericarp extract, in hamster opisthorchiasis. syrian hamsters were divided into four groups: normal (control) (n); administered g. mangostana alone (gm); infected with opisthorchis viverrini alon ...201525836376
quantitative changes in tumor-associated m2 macrophages characterize cholangiocarcinoma and their association with metastasis.the tumor microenvironment (tme) includes numerous non-neoplastic cells such as leukocytes and fibroblasts that surround the neoplasm and influence its growth. tumor-associated macrophages (tams) and cancer-associated fibroblasts (cafs) are documented as key players in facilitating cancer appearance and progression. alteration of the macrophage (cd68, cd163) and fibroblast (α-sma, fsp-1) cells in opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) was here assessed using liver tissues f ...201525854403
synergistic effects of isomorellin and forbesione with doxorubicin on apoptosis induction in human cholangiocarcinoma cell lines.chemotherapy for advanced cholangiocarcinoma (cca) is largely ineffective, but innovative combinations of chemotherapeutic agents and natural compounds represent a promising strategy. in our previous studies, isomorellin and forbesione, caged xanthones isolated from garcinia hanburyi, were found to induce cell cycle arrest and apoptosis in cca cell lines. the subject of our inquiry is the synergistic effect(s) of these caged xanthones with doxorubicin on growth inhibition and apoptosis induction ...201425866479
comparative evaluation of strongyloides ratti and s. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis.the use of strongyloides ratti as heterologous antigen for serodiagnosis of strongyloidiasis is preferable to strongyloides from humans due to the ease and safety of antigen preparation. in southeast asia where opisthorchis viverrini coexists with strongyloides stercoralis, there has been no report in using s. ratti for serodiagnosis of s. stercoralis. in this study, performance of an enzyme-linked immunosorbent assay (elisa) based on s. ratti was compared with that based on s. stercoralis for d ...201525877389
a microrna profile associated with opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma.intrahepatic cholangiocarcinoma (icc) is a highly aggressive tumor of the bile duct, and a significant public health problem in east asia, where it is associated with infection by the parasite opisthorchis viverrini. icc is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority.201525903557
diabetes mellitus: possible risk and promoting factors of cholangiocarcinoma: association of diabetes mellitus and cholangiocarcinoma.the highest incidence of cholangiocarcinoma (cca), a malignancy of bile duct epithelia, is in the northeast of thailand. the liver fluke, opisthorchis viverrini, is the known risk factor for cca development in this region. approximately 1% of o. viverrini infected individuals develop cca. there could be other factors that influence the cholangiocarcinogenesis particularly in the o. viverrini infected individuals. the global epidemiological studies of risk factors for cca in non-o. viverrini rela ...201525910864
seasonal transmission of opisthorchis viverrini sensu lato and a lecithodendriid trematode species in bithynia siamensis goniomphalos snails in northeast thailand.seasonal changes play roles in the transmission success of fish-borne zoonotic trematodes (fzt). this study examined the seasonal transmission patterns of opisthorchis viverrini sensu lato (s.l.) and a virgulate cercaria (family lecithodendriidae) in the snail intermediate host, bithynia siamensis goniomphalos in northeast thailand. snail samples were collected monthly during the rainy, cool, and hot seasons during 2012-2013 to determine the prevalence and intensity of larval trematode infection ...201525918210
re-examination of opisthorchis viverrini infection in northeast thailand.liver fluke infection caused by the parasite opisthorchis viverrini (o. viverrini), a human carcinogen, is endemic in north-eastern thailand and remains a major health problem.201525921154
carcinogenic liver fluke secretes extracellular vesicles that promote cholangiocytes to adopt a tumorigenic phenotype.throughout asia, there is an unprecedented link between cholangiocarcinoma and infection with the liver fluke opisthorchis viverrini. multiple processes, including chronic inflammation and secretion of parasite proteins into the biliary epithelium, drive infection toward cancer. until now, the mechanism and effects of parasite protein entry into cholangiocytes was unknown.201525985904
trematode diversity in the freshwater snail bithynia siamensis goniomphalos sensu lato from thailand and lao pdr.in order to obtain a comprehensive understanding of trematode diversity in bithynia siamensis goniomphalos sensu lato, the first intermediate host of the liver fluke opisthorchis viverrini s.l., the prevalence of larval trematode species was investigated in different localities in thailand and lao people's democratic republic (lao pdr). in thailand, snail samples were collected from 29 localities in the nine provinces: buri ram, surin, chaiya phum, maha sarakham, khon kaen, kalasin, mukdahan, sa ...201625997613
apoptosis of cholangiocytes modulated by thioredoxin of carcinogenic liver fluke.chronic infection with the food-borne liver fluke, opisthorchis viverrini, frequently induces cancer of the bile ducts, cholangiocarcinoma. opisthorchiasis is endemic in thailand, lao pdr, cambodia and vietnam, where eating undercooked freshwater fish carrying the juvenile stage of this pathogen leads to human infection. because inhibition of apoptosis facilitates carcinogenesis, this study investigated modulation by thioredoxin from o. viverrini of apoptosis of bile duct epithelial cells, chola ...201526007234
[role of opisthorchis felineus on induction of bile duct cancer].opisthorchis felineus (trematoda) is widespread in the russian federation, especially in siberia, and other countries of europe. infestation of endemic area population with o. felineus reaches 80%. on animal models of the infection of closely related opisthorchis viverrini combined with the nitrosamines' intake it has been shown that the parasite induces cholangiocarcinoma. however carcinogenic potential of o. felineus is still poorly studied. the present study is aimed to investigate the role o ...201526016330
prevalence and risk factors for infection by opisthorchis viverrini in an urban area of mahasarakham province, northeast thailand.the aim of this cross-sectional analytic study was to investigate the prevalence and risk factors for opisthorchis viverrini (ov) infection in an urban area of northeastern thailand. the participants were 254 household representatives aged 15 years or older living in the most urbanised part of chiang yuen municipality in mahasarakham province. all participants provided stool samples which were examined using the modified kato-katz procedure, and a structured interview questionnaire was used to c ...201526028068
lectin microarray-based sero-biomarker verification targeting aberrant o-linked glycosylation on mucin 1.glycoform of mucin 1 (muc1) in cancerous cells changes markedly with cell differentiation, and thus, qualitative detection and verification of the muc1 glycosylation changes have potential diagnostic value. we have developed an ultrasensitive method to detect the changes in cholangiocarcinoma (cc), which produces muc1, and applied it in the diagnostics development. the focused glycan analysis using 43-lectin-immobilized microarray could obtain the glycan profiles of sialylated muc1 in 5 μl of se ...201526091356
risk factors for opisthorchis viverrini infection in nong khai province, thailand.opisthorchis viverrini (ov) infection is the main risk factor for cholangiocarcinoma and is often found in northeastern thailand. the prevalence of ov infection and the incidence of cholangiocarcinoma are major public health problems in this region.201526107209
[a 55-year old thai male patient with acute right upper quadrant abdominal pain].we report on a 55-year-old thai male who presented during a visit to germany with acute abdominal pain in the right upper quadrant and signs of cholestasis. the diagnosis of liver fluke infection (opisthorchiasis) was confirmed by the detection of the characteristic eggs of opisthorchis viverrini in stool microscopy. the patient was treated with praziquantel. opisthorchiasis is a common parasitic disease and major public health problem in southeast asia and in eastern europe.201526116106
mri and (1)h mrs findings of hepatobilary changes and cholangiocarcinoma development in hamsters infected with opisthorchis viverrini and treated with n-nitrosodimethylamine.3 t mri and (1)h mrs were useful for quantitative investigation of the serial development of hepatobiliary changes in opisthorchis viverrini infection in hamsters, and the differential diagnosis of cholangiocacinoma (cca) development from bile duct changes and normal condition is unclear. in this study, we investigated the serial development of hepatobiliary changes and ccagenesis in o. viverrini-infected and n-nitrosodimethylamine (ndma) treated hamsters (on group) using 3 t mri and (1)h mrs an ...201526117689
excretory/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and il6 production.liver fluke infection caused by opisthorchis viverrini remains a major public health problem in many parts of asia including thailand, lao pdr, vietnam and cambodia, where there is a strikingly high incidence of cholangiocarcinoma (cca - hepatic cancer of the bile duct epithelium). among other factors, uptake of o. viverrini excretory/secretory products (oves) by biliary epithelial cells has been postulated to be responsible for chronic inflammation and proliferation of cholangiocytes, but the m ...201526187786
localization of tubulin from the carcinogenic human liver fluke, opisthorchis viverrini.opisthorchiasis caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao people's democratic republic and thailand. the infection is associated with a number ofhepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, cholelithiasis and cholangiocarcinoma.201526201128
risk areas of liver flukes in surin province of thailand using geographic information system.opisthorchiasis, caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao pdr and thailand.201526201130
data set from the proteomic analysis of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini.the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. this data article contains the results obtained from the analysis of the proteins differentially expressed in the snail b. siamensis goniomphalos upon infection with o. viverrini. it contains the data generated from iquantitator software including a pdf of each sample with a protein׳s relative expression ...201526217698
levels of 8-oxodg predict hepatobiliary pathology in opisthorchis viverrini endemic settings in thailand.opisthorchis viverrini is distinct among helminth infections as it drives a chronic inflammatory response in the intrahepatic bile duct that progresses from advanced periductal fibrosis (apf) to cholangiocarcinoma (cca). extensive research shows that oxidative stress (os) plays a critical role in the transition from chronic o. viverrini infection to cca. os also results in the excision of a modified dna lesion (8-oxodg) into urine, the levels of which can be detected by immunoassay. herein, we m ...201526230769
Refresh
firstprev
123456789
nextlast
Displaying items 601 - 700 of 841