Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [silvan vectors and reservoirs of trypanosoma cruzi. lix. geografic distribution of triatominae in the rio grande hydrographic basin, brazil (hemiptera, reduviidae)]. | 1975 | 1101317 | |
| [ behaviour of a mexican strain of trypanosoma cruzi of particular virulence in the mouse]. | 1975 | 1101332 | |
| letter: trypanosoma cruzi--prenatal transfer of maternal antibody in man. | 1975 | 1101461 | |
| rat liver alcohol dehydrogenase isozymes: influence of infection with trypanosoma. | a study has been made of changes in alcohol dehydrogenase (adh) isozymes in livers of rats infected with the following trypanosomes: trypanosoma cruzi, t. rhodesiense, t. congolense, and six geographical isolates of t. lewisi. a control group of the same age was injected with saline, and another left uninjected. liver homogenates were electrophoresed in noble agar gels followed by formazan staining according to the method of ursprung and leone (1965). of a total of 25 replicates, 15 showed that ... | 1975 | 1101546 |
| epimastigotes and trypomastigotes of trypanosoma cruzi revealed by scanning electron microscopy. | epimastigotes and trypomastigotes of trypanosoma cruzi were observed by scanning electron microscopy. these two forms could easily be differentiated by examination of the undulating membrane and kinetoplast. subpellicular microtubules form parallel helices in epimastigotes while they are arranged parallel to the logitudinal axis in trypomastigotes. some epimastigotes and trypomastigotes showed a "knob-like" swelling at the end of their bodies. | 1975 | 1103885 |
| studies of ribosomal rna of trypanosoma cruzi. | 1975 | 1104797 | |
| serum proteins promoting [3h]thymidine uptake by trypanosoma (schizotrypanum) cruzi (chagas) in vitro. | five proteins capable of stimulating [3h]thymidine uptake by trypanosoma cruzi in vitro were isolated from fetal calf serum by (nh4)2so4 precipitation and ion exchange column chromatography. the proteins were partially characterized by immunodiffusion, immunoelectrophoresis, polyacrylamide gel disc electrophoresis, and sds electrophoresis. as estimated by sds electrophoresis, using 4 standards, the molecular weight of protein 1 was 100,000, that of protein 2 was 76,000, and that of proteins 3-5 ... | 1975 | 1104817 |
| characterization of a natural human antibody with anti-galactosyl(alpha 1-2)galactose specificity that is present at high titers in chronic trypanosoma cruzi infection. | an antibody reactive with the galactosyl(alpha 1-2)galactose [gal(alpha 1-2)gal] epitope was characterized in human sera by enzyme-linked immunosorbent assay, red blood cell (rbc) and laminin absorption, and oligosaccharide inhibition. this antibody was found evenly distributed between the igg and igm classes and was present at high titers in the serum of all normal adults studied, but in 75% of children less than three years of age, it was observed at the lower limit of detection, and gradually ... | 1992 | 1279994 |
| [survival of the human immunodeficiency virus (hiv-1) in triatoma infestans (klug, 1834)]. | triatoma infestans is the main domestic vector of trypanosoma cruzi, the parasitic agent of chagas' disease in south america. we investigated whether triatoma infestans could shelter the hiv-1 virus. for this purpose, we measured the survival time of the virus in the alimentary tract. fifth-instar nymphs of the blood-sucking bug were fed through an ad hoc apparatus with venous blood from asymptomatic hiv-1 seropositive patients. we attempted to evidence the virus by cultivating material from the ... | 1992 | 1280179 |
| polyreactive autoantibodies to negatively charged epitopes following trypanosoma cruzi infection. | during the course of many human autoimmune diseases, antibodies which recognize negatively charged epitopes on self antigens are detected. trypanosoma cruzi, an intracellular protozoan parasite capable of infecting a wide variety of vertebrates, is the cause of chagas disease in humans. infection with the parasite frequently results in autoimmune and inflammatory pathology. we report here on an affinity-purified population of antibodies that bind to a broad class of antigens that contain runs of ... | 1992 | 1280220 |
| fibronectin cleavage fragments provide a growth factor-like activity for the differentiation of trypanosoma cruzi trypomastigotes to amastigotes. | the morphological and biochemical events following trypanosoma cruzi trypomastigote-fibronectin (fn) interactions have been studied. adhesion of trypomastigotes to fn-coated surfaces is followed by fn degradation. the proteolytic cleavage of fn was demonstrated by qualitative and quantitative measurement of fn degradation after its exposure to trypomastigotes as well as polyacrylamide gel analysis of fn proteolysis by a parasite protease (s). the released fn peptide fragments stimulated the tran ... | 1992 | 1281773 |
| substrate specificity of the trypanosoma cruzi trans-sialidase. | trypanosoma cruzi trypomastigotes acquire sialic acid (sa) from host glycoconjugates by means of a plasma membrane-associated trans-sialidase (ts). here we study the substrate specificity of ts, which differs from all known sialyltransferases in that it does not require cytidine monophosphate (cmp)-sa as donor. the t. cruzi ts reversibly transfers sa to saccharides with terminal beta-gal (but not alpha-gal) residues. donors are saccharides with sa linked to terminal beta-gal residues by (alpha 2 ... | 1992 | 1282052 |
| [the use of gentian violet for staining trypanosoma cruzi in tissue]. | 1992 | 1285014 | |
| lethal effect of a bait for rhodnius prolixus (hemiptera: reduviidae), the vector of chagas' disease, containing hexachlorocyclohexane (hch), under laboratory conditions. | the lethal effect of a bait containing an aqueous hexachlorocyclohexane (hch) suspension at the concentration of 1g/l and maintained at room temperature was studied in the laboratory over a period of 12 weeks. the suspension was placed in a latex bag hanging inside a 1000-ml beaker tightly covered with nylon netting, and left there with no changes for 85 days. sixteen groups of r. prolixus bugs, consisting on average of 30 specimens each, were successively exposed to the bait and observed at dif ... | 1992 | 1285256 |
| [the incidence of trypanosoma cruzi antibodies in patients with dilated myocardiopathy at the instituto nacional de cardiología ignacio chávez]. | our objective was to measure the incidence of humoral immune response against trypanosoma cruzi among patients with a definitive diagnosis of dilated cardiomyopathy at the instituto nacional de cardiología "i. chávez" over a year. thirty seven patients were collected, 15 of them (40%) had antibodies against t. cruzi in two different tests, indirect immunofluorescence and elisa, the presence of igg antibody was confirmed through western blot. there was not differences in clinical picture of cardi ... | 1992 | 1285664 |
| specific chemotherapy of chagas disease: a comparison between the response in patients and experimental animals inoculated with the same strains. | eleven strains of trypanosoma cruzi were isolated from patients with chagas disease in central brazil by xenodiagnosis and inoculation into newborn mice. biological characterization and isoenzyme analysis showed that 6 strains were type ii (zymodeme 2) and 5 were type iii (zymodeme 1). patients were treated with benznidazole or benznidazole plus nifurtimox. mice infected with each isolated strain were treated for comparison with the results obtained in the respective patient. evaluation of cure ... | 1992 | 1287919 |
| sero-epidemiological survey for chagas' disease among the xavánte indians of central brazil. | 1992 | 1288441 | |
| activity assays of thiadiazine derivatives on trichomonas vaginalis and amastigote forms of trypanosoma cruzi. | eight thiadiazine 1,1-dioxide derivatives were evaluated for antitrichomonal and antitrypanosomal activities. in vivo tests were performed on a murine model for trichomoniasis standardized in our laboratory. the capacity of compounds to clear visceral lesions in experimentally infected animals as well as their effects on the mortality time of mice were used as criteria for activity. one of the thiadiazines (compound 7b) showed an efficacy similar to that obtained with the reference drug metronid ... | 1992 | 1288970 |
| autonomic neuropathy and immunological abnormalities in chagas' disease. | chagas' disease (american trypanosomiasis) is caused by infection with the haemoflagellate parasite trypanosoma cruzi, which is transmitted from animals to man by the reduviidae bug. the human disease is characterized by two phases. in the first (acute phase) parasitaemia is high and general symptoms variable. the next, which is lifelong (chronic phase), is characterized by inflammatory lesions in cardiac and skeletal muscle, gastrointestinal, the autonomic nervous system. parasites are difficul ... | 1992 | 1290925 |
| isolation of entamoeba histolytica from arthritic knee joint. | amoebae were isolated from an arthritic knee of a male patient. the organisms grew in egg yolk infusion medium, and in diamond's biosate iron-serum-33 medium, which contained trypanosoma cruzi, and were identified microscopically as entamoeba histolytica. furthermore, amoebae-like organisms in aspirate and cultures were immunocytochemically identified using monoclonal antibodies against e. histolytica. on the basis of the morphologic and immunologic observations, it was concluded that the knee l ... | 1992 | 1295146 |
| [viii annual meeting of applied research on chagas disease. uberaba, 22-24 october 1992. abstracts]. | 1992 | 1297158 | |
| trypanosoma cruzi: incorporation of macromolecule precursors during the morphogenic process. | during the morphogenic process from epimastigote to metacyclic trypomastigote stage changes at molecular levels are produced. in the present work the incorporation of macromolecule precursors l [3h]-leucine, [3h]-thymidine and [3h]-uridine was used to monitor the differences between parasites growing with and without specific stimulation for morphogenesis (sepi and nepi, respectively). the peak of maximum [3h]-uridine incorporation was earlier in the sepi than in nepi (3 vs 4 days, respectively) ... | 1992 | 1298017 |
| formal potentials of new analogues of nifurtimox: relationship to activity. | electrochemical studies (formal potentials and coulometry) were performed on several analogues of nifurtimox, in order to gain insight on the proposed mechanism of action of these drugs. it is thought that nifurtimox shows part of its trypanocidal activity via an electron transfer process, leading to the production of active oxygen species that kill the parasite. formal potentials provide relevant data about the feasibility of electron transfer in vivo. all analogues tested contained the nitro g ... | 1992 | 1300126 |
| [differences between patients with right bundle branch block and left anterior hemiblock in chagas myocardiopathy]. | in order to establish whether different kinds of conduction disturbances like complete right branch block (crbb) and left anterior hemiblock (lah) are associated with different degrees of myocardial damage in chagas disease (chd), we studied 25 patients (p), 15 males and 10 women (41 +/- 5 years old) who were divided into five groups: normal ekg 5 p, incomplete right branch block 5 p, crbb-lah 5 p. we performed ekg, phonocardiogram, echo-m, 2-d and doppler. the systolic function was evaluated wi ... | 1992 | 1302286 |
| 83-kilodalton heat shock proteins of trypanosomes are potent peptide-stimulated atpases. | a crithidia fasciculata 83-kda protein purified during a separate study of c. fasciculata trypanothione synthetase was shown to have atpase activity and to belong to the hsp90 family of stress proteins. because no atpase activity has previously been reported for the hsp90 class, atp utilization by c. fasciculata hsp83 was characterized: this hsp83 has an atpase kcat of 150 min-1 and a km of 60 microm, whereas the homologous mammalian hsp90 binds atp but has no atpase activity. crithidia fascicul ... | 1992 | 1304385 |
| didelphis marsupialis: a primary reservoir of trypanosoma cruzi in urban areas of caracas, venezuela. | direct blood examination and xenodiagnosis of 45 sylvatic, peridomestic or domestic mammals from the caracas valley, venezuela, revealed trypanosome infection in six of the 24 opossums, didelphis marsupialis, collected in urban areas. isolates were successfully made of trypanosomes from four of the opossums, using the parasites which developed in rhodnius prolixus fed on the infected opossums to infect nmri mice. the prepatent period, course of parasitaemia, morphology of bloodstream trypomastig ... | 1992 | 1304702 |
| feeding behavior of different species of chagas' disease vectors stimulated with blood sources at different temperatures. | 1. the feeding behavior of six triatomid species toward latex condoms filled with blood at 26 +/- 1 and 36 +/- 1 degrees c was observed for 4 h. 2. the species studied were triatoma infestans, triatoma brasiliensis, triatoma vitticeps, triatoma pseudomaculata, rhodnius prolixus and panstrongylus megistus, and 34 to 348 insects were studied in each group (average, 190). all the life stages of each species were used. 3. although most insects (80%, average for 6 species) preferred the warmer blood, ... | 1992 | 1304939 |
| [simultaneous digestive and cardiological studies in asymptomatic chagasic patients]. | prognosis in patients with chagas disease is related to systemic involvement. 43 asymptomatic chagasic subjects, 28 males and 15 females aged 28 to 85 years, were subjected to electrocardiograms and upper and lower gastrointestinal radiological studies. 23 patients (53%) had evidence of involvement of at least one organ. multiple involvement was demonstrated in 5 individuals (12%): one patient had megaesophagus, megacolon and abnormal ekg; 2 had megaesophagus and abnormal ekg; 1 had megaesophagu ... | 1992 | 1305309 |
| [chagasic infection in blood donors from hospitals in endemic regions of chile (1982-1987). epidemiological impact of the problem]. | chagas' disease, produced by trypanosoma cruzi and transmitted by hematophagous triatomine bugs, exists in the western hemisphere from the south-western united states to central chile and argentina. it exists in rural and periurban sections of the northern half of chile, with a prevalence of 16.9%. constant rural-urban migrations have contributed to its spreading to urban sections. in order to investigate the impact of these migrations on the population susceptible of being blood donors and the ... | 1992 | 1306985 |
| [histotropism and pathogenicity of trypanosoma cruzi in albino mice (nhi) isolated from triatomines in nuevo leon, mexico]. | the neolonese strain of trypanosoma cruzi was obtained from deyections of nosed bugs (triatoma gerstaeckeri) which were caught in general teran and dr. coss, nuevo leon. this strain was kept alive for passing it from mouse-triatomines and vice versa. posteriorly, nests of amastigotes were found in skeletal and myocardial muscle fibers from albinous mouse mus musculus nhi by using light microscopy and electron microscopy. in consequence, the parasitic element was classified as a myotropic strain, ... | 1992 | 1306990 |
| [laboratory study on the immediate and persistent insecticide activity of the pyrethroid lambda-cyhalothrin on nymphs of iv instar triatoma infestans]. | lambda-cyhalothrin (lct) is a new broad-spectrum synthetic pyrethroid insecticide with efficient immediate and persistent activity against a great variety of arthropods harmful both to human and animal health and to vegetal production. in many countries this insecticide has been successfully used in the control of infectious diseases vectors, such as mosquitoes, triatomine bugs (transmitters of trypanosoma cruzi, agent of chagas' disease) and other arthropods. in order to test the immediate and ... | 1992 | 1306993 |
| [current status of knowledge on chagas' disease in the mexican republic]. | a thorough review of the medical literature is made regarding chagas' disease in mexico and elsewhere since 1939, when trypanosoma cruzi was first reported in this country, until 1991. the location where human cases, non human reservoirs and vectors have been found and are pointed out by means of tables and charts. comments are made regarding the results reported. the importance of increasing the studies of chagas' disease in mexican republic is stressed. | 1992 | 1306996 |
| [first report on neotoma micropus (rodentia) as a reservoir of trypanosoma cruzi in mexico]. | using xenodiagnosis, two (8.0%) of 25 woodrats neotoma micropus were found infected with tripanosome parasite in vaquerias, a village in nuevo leon state, mexico. the triatomine species developing infective metacyclic trypanosomes at week 12th were triatoma pallidipennis, t. infestans and t. gerstaeckeri. experimental infections using infected dejections were successfully conducted on laboratory mice (cd1 strain) confirming the vertebrate cycle of trypanosome cruzi. the biological characterizati ... | 1992 | 1306997 |
| [usefulness of the elisa-igg test in sera and filter paper blood eluates in the chagas disease immunodiagnosis]. | an elisa-igg test using a soluble extract of trypanosoma cruzi antigen has been developed. one hundred and seventy eight sera (74 healthy people, 44 chagasic patients with positive xenodiagnosis and 60 with clinical symptoms), and 64 paper filter blood eluates (35 healthy people, 10 chagasic patients and 19 with clinical symptoms) were tested by elisa-igg and an indirect hemagglutination test. both types of methods presented an overall average concordance of 98.3% and 100.0% with sera and filter ... | 1992 | 1307003 |
| [electrocardiographic alterations in persons with a positive serology for toxoplasmosis]. | to each of 11,161 randomly taken people from urban and peri-urban localities of the first seven regions of chile (geographic area of distribution of chagas' disease in the country), with negative serology for chagas' disease, an indirect hemagglutination test (ihat) for toxoplasmosis and an ekg were practiced. the ihat for toxoplasmosis resulted positive in 3,519 individuals (31.5%). the ekg showed alterations in 10.9% of the ihat positive individuals and in 7.9% of the ihat negative ones. this ... | 1992 | 1307005 |
| [prevalence of antibodies to hepatitis c (anti hcv) in blood donors in rio de janeiro, brazil. its relation to alt and anti hbc]. | we have studied 933 volunteer blood donors from may to july, 1990. after a interview and screening tests for syphilis, chagas disease, malaria and hiv, they underwent an enzyme immunoassay for hbsag, anti hbc and anti hcv antibodies. alanine aminotransferase (alt) serum levels were determined by auto analyser. most blood donors were male with mean age of 33 years (19-65). anti hcv prevalence was 3.1% (29 from 933 blood donors). among anti hcv+, blood donors, 44.8% (13/29) had alt 40 ui/l, 31% (9 ... | 1992 | 1307202 |
| [influence of age on lower esophageal sphincter pressure]. | we studied the effect of aging on lower esophageal sphincter (les) pressure of 52 normal subjects, 129 patients with chagas' disease and 63 patients with systemic sclerosis. three groups were compared: with ages between 10 to 29 years, 30 to 49 years and 50 to 70 years. we used a perfused catheter and the station pull-through (spt) technique, at end expiratory phase. there was no difference in les pressure between the three groups in normal subjects (p = 0.72) and patients with systemic sclerosi ... | 2006 | 1307204 |
| [trypanosoma cruzi in the milk of women with chronic chagas disease]. | breast milk secretion is one of the possible alternative mechanisms of transmission of the parasitosis caused by trypanosoma cruzi, but it is still needed to define its importance in terms of public health. on this aim we searched for the presence of this protozoal organism in the colostrum and breast milk of 40 women with chagas' disease, through direct observation, culture and inoculation. we never found parasite; perhaps it might be possible with the use of more efficient procedures and a lar ... | 1992 | 1307397 |
| modulation of parasitemia and antibody response to trypanosoma cruzi by cyclophosphamide in calomys callosus (rodentia, cricetidae). | calomys callosus a wild rodent, previously described as harboring trypanosoma cruzi, has a low susceptibility to infection by this protozoan. experiments were designed to evaluate the contribution of the immune response to the resistance to t. cruzi infection exhibited by c. calossus. animals were submitted to injections of high (200 mg/kg body weight) and low (20 mg/kg body weight) doses of cyclophosphamide on days -1 or -1 and +5, and inoculated with 4 x 10(3) t. cruzi on day o. parasitemia, m ... | 1992 | 1307406 |
| trypanosoma cruzi: course of infection in platelets-depleted mice. | the effect of platelet depletion on the course of trypanosoma cruzi infection in balb/c mice was investigated. thrombocytopenia was achieved by inoculation of rabbit anti-platelet igg during the parasitemic phase of the infection. the number of parasites in the blood of anti-platelet igg treated was significantly higher than that of non-treated control mice, during the phase of high parasitemia. cumulative mortality of platelet-depleted mice was consistently but not significantly higher than tha ... | 1992 | 1307413 |
| [mortality due to chagas disease in são paulo state (brazil): subsidy for the planning of chagasic patient care]. | in order to estimate the size of the population with chagas' disease, an essential parameter for the establishment of an adequate health care policy for this group of patients, data from 1,646 death certificates from the "fundação sistema estadual de análises de dados" in which chagas' disease was mentioned in the state of s. paulo in 1987 were examined. the population under study comprised adults between 15 and 70 years of age. objectives also included the determination of proportional mortalit ... | 1992 | 1307429 |
| [pathogenesis of mitral valve prolapse in patients with chronic chagas disease: study of papillary muscle myocarditis]. | studying the mitral valve in chronic chagas' heart disease, searching a possible cause effect relationship between this condition and valve prolapse. | 1992 | 1307464 |
| [a quantitative analysis of the mastocytes and eosinophilic granulocytes in the myocardium of wistar rats chronically infected by trypanosoma cruzi. a contribution to the knowledge of myocardial fibrosis]. | we did mast cell and eosinophil granulocyte counts in the myocardium of forty chronic chagasic wistar albino rats. we used 10 controls rats and 30 animals with late-stage (8th month) infection of são felipe, y and colombian trypanosoma cruzi strains, with variable degree of chronic myocarditis. we found chronic fibrosing myocarditis (fibrosis) in 40% of the infected animals. it was detected increased mast cell count in the chagasic rats associated with infection and not related with myocardial f ... | 1992 | 1308065 |
| [the epidemiology of chagas' disease in a rural area of the city of teresina, piauí, brazil]. | in the rural areas of teresina, 129 triatomines were captured distributed in (a) artificial ecotopes; a house with one triatoma brasiliensis, one panstrongylus geniculatus, rhodnius pictipes, and one rhodnius prolixus and in a uninhabited chicken house (7 rhodnius nasutus). (b) natural ecotopes; pahus orbignya martiana (41 rhodnius neglectus, 33 rhodnius prolixus and 41 rhodnius nasutus) and copernicia cerifera (3 rhodnius neglectus). the 22.6% of captured triatomines were infected by flagellate ... | 1992 | 1308066 |
| [the experimental infection of cebus apella sp. monkeys with trypanosoma cruzi. its clinical, electrocardiographic and anatomicopathological assessment]. | thirty-two monkeys were captured and adapted to laboratory conditions captives isolated. they were submitted to multiple xenodiagnosis which were negative. twelve were infected intraperitoneally with different strains of t. cruzi (1.10(5) to 5.10(6)). twenty were the control group. between on to six years both the control group and the infected monkeys, were submitted to xenodiagnosis, serological testing clinical examination and electrocardiography. the clinical examination and the electrocardi ... | 1992 | 1308068 |
| [the evaluation of the chance cross reactivity of serological tests for the diagnosis of human immunodeficiency virus and trypanosoma cruzi infections]. | 1992 | 1308070 | |
| [the transmission of trypanosoma cruzi via human milk]. | 1992 | 1308072 | |
| acute central nervous system infection by trypanosoma cruzi and aids. | the acute infection of the cns by trypanosoma cruzi acquired by blood transfusion is uncommon. the concomitance of aids in the patient reported shows the importance of cellular immunity in restriction of this parasite, and reinforces the problem of blood transfusion in endemic zones. | 1992 | 1308418 |
| analytic morphometry of the trypanosoma cruzi (bolivia strain) forms found in the intestine of rhodnius prolixus. | 1992 | 1308547 | |
| experimental chagas' disease in dogs. | this paper describes the development of experimental chagas' disease in 64 out-bred young dogs. twenty-nine animals were inoculated with the be-62 and 35 with be-78 trypanosoma cruzi strains. twenty-six were infected with blood trypomastigotes by different inoculation routes and 38 with metacyclic trypomastigotes from the vector via the conjunctival route. twenty of the 26 dogs infected with blood trypomastigotes were autopsied during the acute phase. eleven died spontaneously and nine were sacr ... | 1992 | 1308556 |
| the epidemiological significance of chagas' disease in women. | little is known about the risks associated with trypanosoma cruzi infection in non-pregnant and pregnant women. from a limited number of studies it appears that in rural areas, parasite rates and rates of serological positivity are similar in both sexes. abnormal ecg tracings are consistently more frequent in men suggesting that immunity to t. cruzi may be different in females. complications arising from chagas' disease in pregnancy are only infrequently reported. evidence for increased risk of ... | 1992 | 1308557 |
| trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells. | phenothiazines were observed to have a direct effect on trypanosoma cruzi and on its in vitro interaction with host cells. they caused lysis of trypomastigotes (50 um/24 h) and, in axenic medium, dose-dependent inhibition of amastigote and, to a lesser extent, epimastigote proliferation. treatment of infected peritoneal macrophages with 12.5 um chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treat ... | 1992 | 1308566 |
| a new host of trypanosoma cruzi from jujuy, argentina: octodontomys gliroides (gervais & d'orbigny, 1844) (rodentia, octodontidae). | to identify wild hosts of trypanosoma cruzi, surveys were conducted in the subandean valleys of jujuy province, argentina, between june 1986 and march 1987. seventy two mammals from 13 different species were examined by xenodiagnosis. fifty two of them were mostly rodents trapped at the localities of maimará, león and tilcara, and the remainder had been kept in captivity at the estación biológica experimental, in jujuy. trypanosoma cruzi infection was detected only in 2 octodontomys gliroides (2 ... | 1992 | 1308567 |
| the threat of reintroduction of natural transmission of chagas' disease in bambuí, minas gerais state, brazil, due to panstrongylus megistus. | in the epidemiological surveillance program in the county of bambuí, minas gerais, between august 1986 and december 1988, 154 panstrongylus megistus were captured by the local population in both peridomicile and intradomicile environments. fifteen (9.8%) of the p. megistus harboured trypanosoma cruzi. precipitin tests showed that the most frequent triatomine blood meal sources were birds, but other sources were dogs, men and cats. the isoenzyme characterization of 13 t. cruzi strains showed that ... | 1992 | 1308572 |
| atrial natriuretic factor in experimental acute chagas' disease. | 1992 | 1308578 | |
| interactions between immunity and chemotherapy in the treatment of the trypanosomiases and leishmaniases. | the immune status of a host infected with trypanosoma spp. or leishmania spp. can play an important role in successful chemotherapy. in animal models, treatment of african trypanosomiasis with difluoromethylornithine or melarsoprol requires an appropriate antibody-mediated immune response. an intact immune system is also necessary for rapid clearance of trypanosomes from the bloodstream following treatment with suramin or quinapyramine. similarly, an efficient cell-mediated immune responses is r ... | 1992 | 1308932 |
| [the assessment of parasitemia in women who are carriers of trypanosoma cruzi infection during and after pregnancy]. | this paper presents an evaluation of the parasitemic profiles of 119 women chronically infected with t. cruzi. xenodiagnosis (xenos) were applied during (465 xenos) and after pregnancy (363 xenos) in order to detect possible variations in parasitemia in these periods. the frequency of positive xenos was greater during than after gestation. otherwise, the frequency of infected triatomines was wore elevated during pregnancy, indicating higher parasitemic levels in this period. only 17% of the stud ... | 1992 | 1308936 |
| [the prevalence, infection indices and feeding habits of the triatominae captured in an area of epidemiological surveillance]. | prevalence of triatomines and their infection by trypanosoma cruzi, were studied during 1974-1981, in the administrative region of campinas, são paulo, brazil. this investigation was continued (1982-1986), blood meal tests with bird, marsupial, rodent and human antisera having been added. panstrongylus megistus, predominant in the region, whose winged instars are often found infected by t. cruzi in inhabited houses, displayed and alimentary eclecticism. from a sample of 7,785, 14.78 percent reac ... | 1992 | 1308951 |
| a trypanosoma cruzi dna sequence amplified from inflammatory lesions in human chagasic cardiomyopathy. | 1992 | 1308995 | |
| trypanosoma cruzi: alteration of camp metabolism following infection of human endothelial cells. | we have previously reported that trypanosoma cruzi infection of endothelial cells results in alterations in the metabolism of ca2+, inositol triphosphate (ip3), and prostacycline (pgi2). in this report, we demonstrate that infection also alters the metabolism of camp. infection of endothelial cells does not significantly alter beta-adrenergic receptor density or affinity, adenylate cyclase activity, and whole-cell camp levels. however, incubation of infected endothelial cells with the phosphodie ... | 1992 | 1309702 |
| the major cysteine proteinase (cruzipain) from trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes. | we demonstrate that cruzipain, the major cysteine proteinase of trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes. restriction enzyme analysis of 20 clones containing complete repeat units of the gene, as well as sequencing of 2 of these clones, and comparison with previously published partial sequences, indicated that the sequence is conserved among the repeat units, although polymorphisms clearly exist. the repeat units contain an intergenic region of 528 ... | 1992 | 1311053 |
| autoantibodies to the ribosomal p proteins react with a plasma membrane-related target on human cells. | autoantibodies to ribosomal p-proteins are present in 12-16% of patients with systemic lupus erythematosus and are associated with neuropsychiatric disease. as the ribosomal p proteins are located in the cytoplasm, the pathogenic effects of their cognate autoantibodies are unclear. in this study affinity-purified anti-p autoantibodies were used to explore the cell surface of several types of human and animal cells. immunofluorescence as well as em immunogold analysis demonstrated, on the surface ... | 1992 | 1313450 |
| direct identification of trypanosoma cruzi natural clones in vectors and mammalian hosts by polymerase chain reaction amplification. | the polymerase chain reaction was used to amplify the highly variable region of the kinetoplast minicircle of trypanosoma cruzi directly in biological samples (feces of infected triatomine bugs, blood samples of experimentally infected mice, and artificially infected human blood samples). hybridization of the amplified dnas with reference stocks representing different genotypes (natural clones) enabled us to characterize the stocks infecting the biological samples under study. the main interest ... | 1992 | 1313657 |
| interleukin-3 induces antimicrobial activity against leishmania amazonensis and trypanosoma cruzi and tumoricidal activity in human peripheral blood-derived macrophages. | the ability of interleukin-3 (il-3) to induce antimicrobial and tumoricidal activity was evaluated. macrophages infected with two intracellular protozoa, leishmania amazonensis or trypanosoma cruzi, were treated with cytokines. il-3 induced a dose-dependent enhancement of microbistasis against leishmanias, and the activity of il-3 (100 ng/ml) was comparable to that of gamma interferon (ifn-gamma) (1,000 u/ml). in addition, il-3 in combination with either granulocyte-macrophage colony-stimulating ... | 1992 | 1314223 |
| a simple and selective method for visualizing the kinetoplast of trypanosomes by means of phosphomolybdic acid and a photographic developer. | trypanosoma cruzi epimastigotes treated with phosphomolybdic acid and the photographic developer rodinal display bright kinetoplasts when unmounted smears are observed using low-magnification phase-contrast objectives. this simple method enables the rapid and accurate identification of kinetoplasts in trypanosomes. | 1992 | 1317034 |
| trypanosoma cruzi: mechanisms of intracellular calcium homeostasis. | regulation of intracellular ca2+ homeostasis was characterized in epimastigote forms of trypanosoma cruzi using the fluorescence probe fura-2. despite an increase in extracellular ca2+, [ca2+]o, from 0 to 2 mm, cytosolic ca2+, [ca2+]i, increased only from 85 +/- 9 to 185 +/- 21 nm, indicating the presence of highly efficient mechanisms for maintaining [ca2+]i. exposure to monovalent na+ (monensin)-, k+ (valinomycin, nigericin)-, and divalent ca2+ (ionomycin)-specific ionophores, uncouplers of mi ... | 1992 | 1317297 |
| ubiquitin of entamoeba histolytica deviates in six amino acid residues from the consensus of all other known ubiquitins. | the amino acid sequence of ubiquitin from entamoeba histolytica, as deduced from a cdna nucleotide sequence, deviated at six positions from the consensus of all other known ubiquitins (ranging from trypanosoma cruzi to homo sapiens). the corresponding residues were scattered over the primary sequence, but came close together on the surface of the folded protein structure. we conclude that (i) e. histolytica branched off very early from the main eukaryotic line, and (ii) this organism may yield c ... | 1992 | 1322840 |
| a shuttle vector which facilitates the expression of transfected genes in trypanosoma cruzi and leishmania. | a trypanosoma cruzi expression vector has been constructed using sequences derived from the flanking regions of the glyceraldehyde 3-phosphate dehydrogenase (ggapdh) genes. the neomycin phosphotransferase (neor) gene was incorporated as a selectable marker. using electroporation we have introduced this vector into both t. cruzi and leishmania cells and conferred g418 resistance. transformation is mediated by large extrachromosomal circular elements composed of head-to-tail tandem repeats of the ... | 1992 | 1324472 |
| phospholipids in trypanosoma cruzi: phosphoinositide composition and turnover. | the polyphosphoinositides from trypanosoma cruzi were isolated by preparative thin-layer chromatography (tlc) and identified. when myo-[3h]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (pi), lysophosphatidylinositol (lysopi), phosphatidylinositol 4-phosphate (pip) and phosphatidylinositol 4,5-bisphosphate (pip2). short-term incubation with 32pi led to higher percentages of incorporation into phosphatidylethanolamine (pe), lysophosp ... | 1992 | 1325592 |
| trypanosoma cruzi: inhibition of metacyclogenesis by mannose. | metacyclogenesis of trypanosoma cruzi epimastigotes was evaluated in a medium supplemented with triatoma infestans intestinal homogenate in the presence of sugars and derivates as are mannose, galactose, fucose, n-acetylglucosamine, mannose 6-p, and fructose 1,6-p at a concentration of 25 mm. only mannose significantly inhibited metacyclogenesis. sodium metaperiodate and trypsin treatment of the intestinal homogenate also inhibited differentiation. in our opinion there exists a proteinic factor ... | 1992 | 1325784 |
| phospholipids of trypanosoma cruzi: increase of polyphosphoinositides and phosphatidic acid after cholinergic stimulation. | we have studied the effect of carbamoylcholine in trypanosoma cruzi epimastigote forms prelabelled with [32p]-pi. suspensions of cells were incubated at 28 degrees c to measure changes in the levels of [32p]-labelled phospholipids after stimulation. the presence of this cholinergic agonist induced changes in the phosphoinositide metabolism; a shift in the levels of phosphatidylinositol 4,5-bisphosphate (pip2), phosphatidylinositol 4-phosphate (pip) and phosphatidic acid (pa) was observed, wherea ... | 1992 | 1326469 |
| polymorphism of the 35- and 50-kilodalton surface glycoconjugates of trypanosoma cruzi metacyclic trypomastigotes. | in this study, we examined the immunochemical properties of the 35- and 50-kda (35/50-kda) surface glycoconjugates expressed on the surface of metacyclic trypomastigotes of trypanosoma cruzi using three different monoclonal antibodies directed to this component. the 35/50-kda surface antigen was expressed by metacyclic trypomastigotes of 11 different strains of t. cruzi and displayed a significant degree of molecular polymorphism. results of immunoblotting and complement-mediated lysis were cons ... | 1992 | 1328061 |
| activation of human macrophages for the killing of intracellular trypanosoma cruzi by tnf-alpha and ifn-gamma through a nitric oxide-dependent mechanism. | the protozoan parasite trypanosoma cruzi is able to replicate in the cytoplasm of primary resident macrophages, but is killed by activated macrophages. pretreatment of human macrophages with recombinant ifn-gamma and to a lesser extent with tnf-alpha, induced a significant trypanocidal activity. furthermore, tnf-alpha had a synergistic effect with ifn-gamma on macrophage activation in t. cruzi killing. similarly, ifn-gamma triggered the production of nitric oxide (no) by macrophages, whereas tnf ... | 1992 | 1330900 |
| cloning and characterization of the gene encoding trypanosoma cruzi dna topoisomerase ii. | the gene encoding trypanosoma cruzi type ii topoisomerase (tctop2) was isolated from a genomic library with a heterologous probe corresponding to part of the trypanosoma brucei type ii topoisomerase (tbrtop2) gene. nucleotide sequencing of tctop2 showed that the gene consists of an open reading frame of 3696 nucleotides (1232 amino acids), predicting a polypeptide product of 138,413 da. comparison of the amino acid sequence with that of type ii topoisomerases from t. brucei (tbrtop2) and crithid ... | 1992 | 1331785 |
| characterization of trypanosoma cruzi isolates from paraguay, using restriction enzyme analysis of kinetoplast dna. | eleven paraguayan strains of trypanosoma cruzi, from chagas' disease patients and the bug vectors, were examined by restriction endonuclease analysis of kinetoplast dna using hae iii, msp i, eco ri, hinfi, taq i and rsa i. four schizodeme-profile groups were identified. group 1 had much simpler profiles than groups 2, 3 and 4 and, although there were homogeneous profiles in the latter three groups, each group could be distinguished from the others. the profiles of group 1 could not be matched wi ... | 1992 | 1333178 |
| [itraconazole action on toxoplasma gondii]. | with the purpose to increase our knowledge about the spectrum of therapeutic action of itraconazole, the activity of a new triazolic derivative against toxoplasma gondii was studied in vivo and in vitro with the aid of an experimental infection model in mice. the trial also aimed at a possible improvement of the therapy of toxoplasmosis in regard to safety and dosing easiness. the present study has not shown significant activity of itraconazole against toxoplasma gondii, differing from a previou ... | 1992 | 1339150 |
| endocytosis of albumin-gold particles by trypanosoma cruzi infected and non infected heart muscle cells. | 1992 | 1339215 | |
| scintigraphic study of the gastrointestinal transit of a liquid meal in patients with chronic chagas' disease. | gastric emptying and small bowel transit of a liquid meal (isotonic dextrose) were assessed by a scintigraphic technique in 16 patients with chagas' disease involving the esophagus and/or the colon, including one case with mega-jejunum, and in 10 control subjects. initial gastric emptying was faster in the chagas' disease group than in controls (gastric retention at 15 min, median and (range): 52% (15-86) vs 71% (43-97), p < 0.01) although there was no significant difference in total gastric emp ... | 1986 | 1339510 |
| [heart transplantation: donor with chagas' disease and clinical course of the receptor]. | because of an operational misshape, a female patient received a heart transplant whose donor was chronically infected by trypanosoma cruzi. after 3 months, transmission of the parasite has not been detected, what deserves communication, considering the clinical and scientific implications of the case. | 1992 | 1340020 |
| [evaluation of an elisa technique for detection of antigens and circulating immune complexes of trypanosoma cruzi by a field study in an endemic zone of argentina]. | the elisa technique for detection of t. cruzi circulating antigens (cag) and immune complexes (cic) in the sera of chronic chagasic patients was field-tested in a well-known endemic area of argentina (san luis province). of 215 individuals screened, 51 were positive for elisa-cic and 45 for elisa-cag. seventy four subjects were considered por t. cruzi-infected, as they showed serologic reactivity at least by two different techniques. in this group, 49 (66.21%) were elisa-cic positive, whereas in ... | 1992 | 1340028 |
| [evaluation of the therapeutic activity of itraconazole in chronic infections, experimental and human, by trypanosoma cruzi]. | other authors have demonstrated that itraconazole has antiparasitic activity against trypanosoma cruzi both in vitro and in animal acute infection. because of these observations, we decided to evaluate the chronic phase of this protozoal disease, since it is the most important form under a clinical and assistential point of view. we studied an infected mouse model as well as human cases of chagas' disease. one hundred mg/kg/day by gastric tube, and 100 or 200 mg/day orally were given, respective ... | 1992 | 1340033 |
| [a longitudinal radiologic study of the esophagus in an endemic area of chagas' disease over a 6-year period]. | a longitudinal study over six years was undertaken of 494 residents of the municipality of mambaí, goiás. two hundred and twelve (43%) were seropositive in 1975/76 and 199 of 464 of the same patients group, positive in 1980/82 (42.8%). at both examination single radiographs of the oesophagus were obtained immediately after ingestion of 75 ml of barium sulphate solution and a second x-ray taken one minute later. among the 201 seropositive patients without megaesophagus in the first study 4 (2%) e ... | 2016 | 1340535 |
| [cimex lectularius l. (the common bedbug), the vector of trypanosoma cruzi]. | 1992 | 1340545 | |
| [chagas disease with the acquired immunodeficiency syndrome. clinical cases]. | we report 2 patients with aids who developed chagas infection, one with encephalitis, the other with acute myocarditis. the implications of immune depression for the manifestations and course of chagas disease are discussed. chagas disease should be considered in patients with aids who live in endemic zones and who develop cerebral or cardiac manifestations. | 1992 | 1340556 |
| [evaluation of commercially available reagents for diagnosis of chagas disease in chilean blood banks. i. selection of reagents]. | infection with t cruzi through blood transfusion is an important public health problem in latin america, including chile. a study on the sensitivity, specificity and applicability of currently applied methods was performed. ten commercially available products were tested in a panel of 180 blood specimens against the indirect immunofluorescence antibody test (ifat) and the immunoperoxidase test (ip). qualitative concordance ranged from 82 to 98%, sensitivity from 64 to 97% and specificity from 93 ... | 1992 | 1340572 |
| [evaluation of the therapeutic activity of fluconazole in acute experimental infection caused by trypanosoma cruzi]. | on hand of information that fluconazole can inhibit trypanosoma cruzi, infection, an experimental investigation in acutely infected mice treated with 200 mg/kg daily of this drug during one month was carried out. parasitemia patency, blood culture, sub-inoculation and histologic examination were the parameters used to evaluate the potential therapeutic activity of the drug. the results did not confirm the hypothesis, but it would be desirable to conduct new trials with this and other imidazole c ... | 1992 | 1340597 |
| [sudden death in chagas' disease]. | 1992 | 1341144 | |
| [the pathogenesis of mitral valve prolapse in chronic chagas disease patients: a study of myocarditis of the papillary muscles]. | 1992 | 1341178 | |
| bacterial chemistry. vi. biological activities and cytotoxicity of 1,3-dihydro-2h-indol-2-one derivatives. | the biosynthetic pigment from chromobacterium violaceum bb-78, 1,3-dihydro-2h-indol-2-one and its derivatives exhibit biological activities such as antimicrobial action, low hemolytic effects on red blood cells and in vitro trypanocide activity. a relatively high cytotoxicity on v-79 hamster fibroblast cells of the biosynthetic pigment was found, although with the methylol derivative the toxicity was almost eliminated. the methylol derivative exhibited similar toxicity as nifurtimox, a known, co ... | 1992 | 1341576 |
| [quality control of commercial kits for the serologic diagnosis of chagas disease]. | 1992 | 1341784 | |
| embryology of triatoma infestans (klug), (hemiptera, reduviidae), a chagas' disease vector. | this study reports the embryogenesis of t. infestans (hemiptera, reduviidae). morphological parameters of growth sequences from oviposition until hatching (12-14 d 28 degrees c) were established. five periods, as percent of time of development (td), were characterized from oviposition until hatching. the most important morphological features were: 1) formation of blastoderm within 7% of td; 2) germ band and gastrulation within 30% of td; 3) nerve cord, limb budding, thoracic and abdominal segmen ... | 1992 | 1342072 |
| immune response in mice immunized with acidic antigenic fractions from trypanosoma cruzi cytosol. | the humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of t. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, f iii and f iv, plus bordetella pertussis as adjuvant. the immunization with f iii induced positive ith and dth responses to homologous antigens. in mice immunized with f iv, the ith was negative and four out of six animals presented positive dth reactions. in both groups of mice the analysis of i ... | 1992 | 1342100 |
| possible risk factors for vertical transmission of chagas' disease. | the author emphasizes the importance of the congenital transmission of chagas' disease and discusses the possible risk factors for transmission such as age, origin, obstetrical history and maternal form of disease. exacerbation of infection during pregnancy is also considered as a possible risk factor for transmission. besides, a relationship between the frequency of transmission and gestational age is presented. concerning breast-feeding, the risk of transmission is directly related to the acut ... | 2005 | 1342103 |
| gastric acid secretion response in the cebus apella. a monkey model of chronic chagas' disease. | the objective was to study the secretory pattern, both basal and stimulated either by histamine (0.1 mg/kg) or pentagastrin (64 micrograms/kg) in eighteen cebus apella monkeys chronically infected with different t. cruzi strains (ca1, n = 10; colombian, n = 4 and tulahuen, n = 4) and to describe the morphological findings in the gastrointestinal tract in twelve infected (6 sacrificed and 6 spontaneously dead) and four healthy monkeys. all infected monkeys and 35 healthy ones were evaluated by co ... | 1992 | 1342116 |
| [the pathogenesis of chronic chagas' myocarditis: the role of autoimmune and microvascular factors]. | the pathogenesis of chronic chagasic myocarditis remains incompletely understood. several hypotheses have been proposed: (1) direct tissue destruction by trypanosoma cruzi; (2) neurogenic theory; (3) anti-heart immune reactions; and (4) microvascular disease. we present herein a dynamic alternative hypothesis. we believe that the development of myocarditis is related to progressive and additive focal cellular necrosis, and associated reactive and reparative myocardial fibrosis and surrounding my ... | 1992 | 1342130 |
| the acute phase of experimental infection with trypanosoma cruzi is more severe in mice monoassociated with strict anaerobic bacteria. | 1. the influence of some components of the normal human intestinal flora on the acute phase of experimental infection with strain cl of trypanosoma cruzi was studied in 30-day-old germ-free or gnotobiotic cfw (lob) mice monoassociated with bacteroides fragilis, peptostreptococcus sp or clostridium sp by intragastric inoculation of 10(6) bacteria 10 days before the intraperitoneal infection with 5 x 10(3) trypomastigotes/g body weight. 2. significantly earlier parasitemia peak and mortality were ... | 1992 | 1342224 |
| [genetic evidence corroborates negme's hypothesis about the greater mildness of american trypanosomiasis in chile]. | chagas disease is present in northern and central chile. not more than 25% of infected individuals have had a pathologic condition presumably due to trypanosoma cruzi. the majority of individuals with chronic infectious chagas disease in chile are asymptomatic in contrast to what has been observed in other south american countries. historic and paleopathologic evidences suggest that this particular behavior could be explained by a genetic adaptation of chilean aborigines to t cruzi. associations ... | 1992 | 1342473 |
| [transfusional chagas infection detected in the program for the control of chagas disease in the state of são paulo (brazil)]. | a system of surveillance for chagas' disease aiming at a systematic investigation of the occurrence of triatominae in human dwellings in s. paulo, brazil was proposed. it included a serological survey of residents in house considered to be potential breeding places for blood-sucking triatomines. serologically positive cases were observed to be distributed in age groups from 19 years of age upwards. case-investigation revealed that the infection had been acquired either in s. paulo in the past or ... | 1992 | 1342502 |
| effect of an essential fatty acid deficient diet on experimental infection with trypanosoma cruzi in germfree and conventional mice. | 1. to investigate the possible role of essential fatty acid deficiency in host cell/parasite interaction, weanling germfree (gf) and conventional (cv) cfw mice maintained on an essential fatty acid-deficient (-) or a control (+) diet for 110 days were infected with trypanosoma cruzi. 2. blood parasitemia indicated that the disease was milder in the animals maintained on the essential fatty acid-deficient diet than in the gf and cv controls (maximum parasitemia: gf+ 33,300, gf-26,200, cv+ 17,100 ... | 1992 | 1342611 |
| [triatoma infestation in the san luis del palmar department of corrientes province, argentina]. | the purpose of this study was to determine domestic and peridomestic infestation of triatominae, trypanosoma cruzi infection rates, and the prevalence of human seroreactivity in rural areas of the department of san luis del palmar, corrientes, argentina (fig. 1). the study took place from april 1986 to may 1989. the department of san luis del palmar covers an area of 2551 km2 and is part of the neotropical biogeographic region, chacoan dominion and province. the climate is of the subhumid type. ... | 1992 | 1342684 |