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leaching and half-life of the herbicide tebuthiuron on a recharge area of guarany aquifer in sugarcane fields in brazil.this study was undertaken to evaluate the degradation and mobility of the herbicide tebuthiuron (n-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-n,n'-dimethylurea) in soil under field conditions, and its potential for leaching and groundwater contamination. a watershed, espraiado, located over a recharge area in brazil, was chosen for soil and water studies. at espraiado, water samples were collected from seven wells at intervals of three months from march 2004 to june 2006 and analyzed for tebu ...200717701698
isolation and characterization of xylitol-producing yeasts from the gut of colleopteran insects.a total of 35 yeasts were isolated from the gut of beetles collected from hyderabad city, india. twenty of these yeasts utilized xylose as a sole carbon source but only 12 of these converted xylose to xylitol. the ability to convert xylose to xylitol varied among the isolates and ranged from 0.12 to 0.58 g/g xylose. based on the phenotypic characteristics and phylogenetic analysis of the d1/d2 domain sequence of 26s rrna gene, these isolates were identified as members of pichia, candida, issatch ...200717710483
photosynthetic pathway and biomass energy production.the current interest in locating new or alternative sources of energy has focused attention on solar energy capture by crops that can be subsequently utilized as a substitute for fossil fuels. the very high productivity of sugarepane and the fact that it accumulates sugars that are directly fermentable to alcohol may have caused seemingly less productive crops to be overlooked. we show here that recoverable alcohol from achievable commercial yields of pineapple can actually equal that of sugarca ...197917729660
[trapping techniques for solenopsis invicta].a field study was made to investigate the trapping effects of different attractants, traps, and wind directions on solenopsis invicta. the results showed that among the test attractants, tb1 (50 g fishmeal, 40 g peptone, 10 ml 10% sucrose water solution and 20 ml soybean oil) had the best effect, followed by tb2 (ham), tb6 (100 g cornmeal and 20 ml soybean oil) and tb4 (10 ml 10% sucrose water solution, 100 g sugarcane powder and 20 ml soybean oil), with a mean capture efficiency being 77.6, 58. ...200717763750
domestication to crop improvement: genetic resources for sorghum and saccharum (andropogoneae).both sorghum (sorghum bicolor) and sugarcane (saccharum officinarum) are members of the andropogoneae tribe in the poaceae and are each other's closest relatives amongst cultivated plants. both are relatively recent domesticates and comparatively little of the genetic potential of these taxa and their wild relatives has been captured by breeding programmes to date. this review assesses the genetic gains made by plant breeders since domestication and the progress in the characterization of geneti ...200717766842
simultaneous saccharification and fermentation of steam-pretreated bagasse using saccharomyces cerevisiae tmb3400 and pichia stipitis cbs6054.sugarcane bagasse--a residue from sugar and ethanol production from sugar cane--is a potential raw material for lignocellulosic ethanol production. this material is high in xylan content. a prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol. in the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (ssf) set-up using either saccharomyces cerevisi ...200817787015
inhibition of agrobacterium-induced cell death by antiapoptotic gene expression leads to very high transformation efficiency of banana.the death of plant cells in culture following exposure to agrobacterium tumefaciens remains a major obstacle in developing agrobacterium-mediated transformation into a highly efficient genotype-independent technology. here, we present evidence that a. tumefaciens exposure induces cell death in banana cell suspensions. more than 90% of embryogenic banana cells died after exposure to a. tumefaciens and cell death was accompanied by a subset of features associated with apoptosis in mammalian cells, ...200717849707
a two-dimensional proteome reference map of herbaspirillum seropedicae proteins.herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. here, we present a 2-d reference map for h. seropedicae. using maldi-tof-ms we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. seventeen hypothetical or conserved hypothetical orfs were shown to code for true gene products. these data will support the genome annotation process and provide a basis on which ...200717853511
solid-state fermentation for gluconic acid production from sugarcane molasses by aspergillus niger arnu-4 employing tea waste as the novel solid support.solid-state fermentation (ssf) was evaluated to produce gluconic acid by metal resistant aspergillus niger (arnu-4) strain using tea waste as solid support and with molasses based fermentation medium. various crucial parameters such as moisture content, temperature, aeration and inoculum size were derived; 70% moisture level, 30 degrees c temperature, 3% inoculum size and an aeration volume of 2.5l min(-1) was suited for maximal (76.3 gl(-1)) gluconic acid production. non-clarified molasses base ...200817881224
construction of a genetic linkage map for saccharum officinarum incorporating both simplex and duplex markers to increase genome coverage.saccharum officinarum l. is an octoploid with 80 chromosomes and a basic chromosome number of x = 10. it has high stem sucrose and contributes 80% of the chromosomes to the interspecific sugarcane cultivars that are grown commercially for sucrose. a genetic linkage map was developed for s. officinarum (clone ij76-514) using a segregating population generated from a cross between q165 (a commercial sugarcane cultivar) and ij76-514. in total, 40 aflp and 72 ssr primer pairs were screened across th ...200717893734
diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates.bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ...200717905875
identification and sequence analysis of potyviruses infecting crops in vietnam.fifty-two virus isolates from 13 distinct potyvirus species infecting crops in vietnam were identified and the 3' region of each genome was sequenced. the viruses were: bean common mosaic virus (bcmv), potato virus y (pvy), sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv), chilli veinal mottle virus (chivmv), zucchini yellow mosaic virus (zymv), leek yellow stripe virus (lymv), shallot yellow stripe virus (sysv), onion yellow dwarf virus (oydv), turnip mosaic virus (tumv), dasheen mosa ...200817906829
influence of vermicomposting on solid wastes decomposition kinetics in soils.the effect of vermicomposting on kinetic behavior of the products is not well recognized. an incubation study was conducted to investigate c mineralization kinetics of cow manure, sugarcane filter cake and their vermicomposts. two different soils were treated with the four solid wastes at a rate of 0.5 g solid waste c per kg soil with three replications. soils were incubated for 56 d. the co(2)-c respired was monitored periodically and a first-order kinetic model was used to calculate the kineti ...200717910115
d-003 does not possess oestrogenic potential in-vivo: findings of the uterotrophic assay.d-003 is a mixture of long-chain fatty acids purified from sugarcane wax that inhibits both cholesterol synthesis prior to mevalonate formation, and lipid peroxidation. d-003 has been shown to prevent bone loss and bone resorption in ovariectomized rats, and significantly improves bone resorption markers in postmenopausal women with reduced bone mineral density. as hormone-replacement therapy, d-003 displays cholesterol-lowering and anti-resorptive effects. we have studied its potential oestroge ...200717910820
f2 screen for resistance to a bacillus thuringiensis-maize hybrid in the sugarcane borer (lepidoptera: crambidae).a novel f2 screening technique was developed for detecting resistance in sugarcane borer, diatraea saccharalis (f.), to transgenic bacillus thuringiensis (bt)-maize expressing the cry1ab insecticidal protein. the f2 screening method involved (i) collecting larvae from maize fields; (ii) establishing two-parent families; (iii) screening f2 neonates for survival on bt-maize leaf tissues; and (iv) confirming resistance on commercial bt-maize plants. with the f2 screening method, 213 iso-line famili ...200717916262
influence of distillery effluent on germination and growth of mung bean (vigna radiata) seeds.distillery effluent or spent wash discharged as waste water contains various toxic chemicals that can contaminate water and soil and may affect the common crops if used for agricultural irrigation. toxic nature of distillery effluent is due to the presence of high amounts of organic and inorganic chemical loads and its high-acidic ph. experimental effects of untreated (raw) distillery effluent, discharged from a distillery unit (based on fermentation of alcohol from sugarcane molasses), and the ...200817928137
isolation of nucleotide binding site-leucine rich repeat and kinase resistance gene analogues from sugarcane (saccharum spp.).resistance gene analogues (rgas) have been isolated from many crops and offer potential in breeding for disease resistance through marker-assisted selection, either as closely linked or as perfect markers. many r-gene sequences contain kinase domains, and indeed kinase genes have been reported as being proximal to r-genes, making kinase analogues an additionally promising target. the first step towards utilizing rgas as markers for disease resistance is isolation and characterization of the sequ ...200817935262
long-term carcinogenicity of d-003, a mixture of high molecular weight acids from sugarcane wax, in sprague dawley rats: a 24 months study.d-003 is a mixture of high molecular weight sugarcane wax aliphatic primary acids with cholesterol-lowering, anti-platelet and antioxidant effects. this study investigated the long-term oral toxicity and carcinogenicity of d-003 in sprague dawley rats of both sexes, randomly distributed into four groups: a control group, treated only with the vehicle, and three treated with d-003 (50, 500 and 1500 mg/kg). all treatments were given orally for 24 months. mortality (survival analysis), clinical sym ...200717937974
phenolic compounds from sugarcane molasses possessing antibacterial activity against cariogenic bacteria.during the course of our research into the use of cane by-products from sugar manufacturing, we have studied the isolation and structural determination of bioactive compounds present in sugarcane molasses. in this study, dehydrodiconiferylalcohol-9'-o-beta-d-glucopyranoside (1) and isoorientin-7, 3'-o-dimethyl ether (2) were isolated as antibacterial active compounds against cariogenic bacteria. their structures were elucidated by (1)h-nmr, (13)c-nmr and esi-ms. the activities of these isolated ...200717938552
impact of filter mud applications on the germination of sugarcane and small-seeded plants and on soil and sugarcane nitrogen contents.the impact of filter mud (fm) on the germination of sugarcane (saccharum spp.), guar (cyamopsis tetragonoloba), maize (zea mays), groundnut (arachis hypogaea) and sesame (sesamum indicum), and the nitrogen content of soil and sugarcane were investigated. experiments were set up in 2004 at the kenana sugarcane estate, sudan. for this study, 1:0,3:1,1:1,1:3 and 0:1 fm/soil (w/w) mixtures were used. the results showed that fm had no adverse impact on germination. soil organic carbon and total nitro ...200817945488
variation in the nature of organic acid secretion and mineral phosphate solubilization by citrobacter sp. dhrss in the presence of different sugars.a novel phosphate solubilizing bacterium (psb) was isolated from the rhizosphere of sugarcane and is capable of utilizing sucrose and rock phosphate as the sole carbon and phosphate source, respectively. this psb exhibited mineral phosphate solubilizing (mps) phenotype on sugars such as sucrose and fructose, which are not substrates for enzyme glucose dehydrogenase (gdh), along with gdh substrates, viz., glucose, xylose, and maltose, as carbon sources. pcr amplification of the rrna gene and sequ ...200817965911
identification of new aba- and meja-activated sugarcane bzip genes by data mining in the sucest database.sugarcane is generally propagated by cuttings of the stalk containing one or more lateral buds, which will develop into a new plant. the transition from the dormant into the active stage constitutes a complex phenomenon characterized by changes in accumulation of phytohormones and several other physiological aspects. abscisic acid (aba) and methyl-jasmonate (meja) are major signaling molecules, which influence plant development and stress responses. these plant regulators modulate gene expressio ...200817968554
effects of dietary fibers on weight gain, carbohydrate metabolism, and gastric ghrelin gene expression in mice fed a high-fat diet.diets that are high in dietary fiber are reported to have substantial health benefits. we sought to compare the metabolic effects of 3 types of dietary fibers -- sugarcane fiber (scf), psyllium (psy), and cellulose (cel) -- on body weight, carbohydrate metabolism, and stomach ghrelin gene expression in a high-fat diet-fed mouse model. thirty-six male mice (c57bl/6) were randomly divided into 4 groups that consumed high-fat diet alone (hfd) or high-fat diet containing 10% scf, psy, and cel, respe ...200717998014
cell immobilization and xylitol production using sugarcane bagasse as raw material.sugarcane bagasse pretreated by three different procedures (with 2% [v/v] polyethyleneimine (pei), with 2% [w/v] naoh, or with a sequence of naoh and pei) was used as cell immobilization carrier for xylitol production by candida guilliermondii yeast. fermentations using these pretreated carriers were performed in semidefined medium and in a hydrolysate medium produced from sugarcane bagasse hemicellulose. sugarcane bagasse pretreated with naoh was the best carrier obtained with respect to immobi ...200718025553
histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae).the silk gland in lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. the secretion of fibroin and sericin by the different silk gland regions are well established. there are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. this study characterizes the glandular region and detects the presence of lipid components in the secr ...200718060296
validation of a tn5 transposon mutagenesis system for gluconacetobacter diazotrophicus through characterization of a flagellar mutant.gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. the genome of strain pal5 of g. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. the aim of this study was to establish an efficient mutagenesis method, using the commercial tn5 transposon ez::tn5<kan-2>tnp transposome (epicentre). up to 1 x 10(6) mutants per microgram of transposome were generated in a ...200818060666
ultrastructure of the excretory duct in the silk gland of the sugarcane borer diatraea saccharalis (lepidoptera: pyralidae).the excretory duct in the silk gland of the sugarcane borer diatraea saccharalis consists of two morphologically distinct regions, recognized by scanning and transmission electron microscopy. the thin posterior region, adjacent to the glandular region, presents a regular surface. secretory vesicles containing either electron-dense or fibrillar cuticular-like materials are observed in their apical cytoplasm; the same cuticular materials were detected as extracellular deposits among the microvilli ...200218088967
effects of emulsified policosanols with different chain lengths on cholesterol metabolism in heterozygous ldl receptor-deficient mice.policosanol is a mixture of long-chain primary aliphatic saturated alcohols. previous studies in humans and animals have shown that these compounds improved lipoprotein profiles. however, more-recent placebo-controlled studies could not confirm these promising effects. octacosanol (c28), the main component of sugarcane-derived policosanol, is assumed to be the bioactive component. this has, however, never been tested in an in vivo study that compared individual policosanol components side by sid ...200818162663
novel sugarcane streak and sugarcane streak réunion mastreviruses from southern africa and la réunion.the sugarcane infecting streak viruses (sisvs) are mastreviruses (family geminiviridae) belonging to a group of "african streak viruses" (afsvs) that includes the economically devastating maize streak virus (msv). although there are three currently described sisv species (sugarcane streak virus [ssv], sugarcane streak egypt virus [ssev] and sugarcane streak réunion virus [ssrv]), only one strain variant has been fully sequenced for each of these species and as a result very little is known about ...200818175043
semi-continuous xylose-to-xylitol bioconversion by ca-alginate entrapped yeast cells in a stirred tank reactor.candida guilliermondii fti 20037 cells were entrapped in ca-alginate beads and used for xylose-to-xylitol bioconversions during five successive batches in a stirred tank reactor. supplemented sugarcane bagasse hemicellulosic hydrolysate was used as the fermentation medium. the average volume of the ca-alginate beads was reduced by about 30% after the 600 h taken to perform the five bioconversion cycles, thus demonstrating physical instability under the conditions prevailing in the reactor vessel ...200818175152
recent advances in nitrogen-fixing acetic acid bacteria.nitrogen is an essential plant nutrient, widely applied as n-fertilizer to improve yield of agriculturally important crops. an interesting alternative to avoid or reduce the use of n-fertilizers could be the exploitation of plant growth-promoting bacteria (pgpb), capable of enhancing growth and yield of many plant species, several of agronomic and ecological significance. pgpb belong to diverse genera, including azospirillum, azotobacter, herbaspirillum, bacillus, burkholderia, pseudomonas, rhiz ...200818177965
inhibitory effect of the sugarcane cystatin canecpi-4 on cathepsins b and l and human breast cancer cell invasion.the lysosomal cysteine proteases cathepsin b and l play important roles in tumor cell invasion. an imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. accordingly, many studies have indicated potential use of cystatins in therapeutic approaches. we report a novel cystatin from sugarcane (saccharum officinarum), canecpi-4, with strong inhibitory activity against cathepsins b (k(i) = 0.83 nm) and l (k( ...200818208350
evaluation of transgenic bacillus thuringiensis corn hybrids against cry1ab-susceptible and -resistant sugarcane borer (lepidoptera: crambidae).a louisiana strain of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), was selected for resistance to the crylab protein of bacillus thuringiensis (bt) by using an f2 screening procedure. survival of bt-resistant, -susceptible, and -heterozygous genotypes of sugarcane borer was evaluated on vegetative and reproductive stages of five non-bt and seven bt field corn, zea mays l., hybrids in a greenhouse study. larval survival was recorded 21 d after infestation of neonates o ...200718232406
[comparative qtl mapping of resistance to sugarcane mosaic virus in maize based on bioinformatics].the development of genomics and bioinformatics offers new tools for comparative gene mapping. in this paper, an integrated qtl map for sugarcane mosaic virus (scmv) resistance in maize was constructed by compiling a total of 81 qtl loci available with the genetic map ibm2 2005 neighbors as reference. these 81 qtl loci were scattered on 7 chromosomes of maize, and most of them was clustered on chromosome 3 and 6. by using meta- analysis method, we identified one and two "consensus qtls" on chromo ...200818244910
effect of carbon sources on the induction of xylanolytic-cellulolytic multienzyme complexes in paenibacillus curdlanolyticus strain b-6.the effect of polymeric substances such as alpha-cellulose, birchwood xylan, corn hull, and sugarcane bagasse, and of soluble sugars such as l-arabinose, d-galactose, d-glucose, d-xylose, and cellobiose, on the induction of multienzyme complexes in a facultatively anaerobic bacterium, paenibacillus curdlanolyticus b-6, was investigated under aerobic conditions. cells and culture supernatants of strain b-6 grown on different carbon sources were analyzed. cells grown on each carbon source adhered ...200818256505
genetic analysis for brix weight per stool and its component traits in sugarcane (saccharum officinarum).brix weight per stool (bw) of sugarcane is a complex trait, which is the final product of a combination of many components. diallel cross experiments were conducted during a period of two years for bw and its five component traits, including stalk diameter (sd), stalk length (sl), stalk number (sn), stalk weight (sw), and brix scale (bs) of sugarcane. phenotypic data of all the six traits were analyzed by mixed linear model and their phenotype variances were portioned into additive (a), dominanc ...200718257118
dna gyrase from the albicidin producer xanthomonas albilineans has multiple-antibiotic-resistance and unusual enzymatic properties.the sugarcane pathogen xanthomonas albilineans produces a family of antibiotics and phytotoxins termed albicidins, which inhibit plant and bacterial dna gyrase supercoiling activity, with a 50% inhibitory concentration (50 nm) comparable to those of coumarins and quinolones. here we show that x. albilineans has an unusual, antibiotic-resistant dna gyrase. the x. albilineans gyra and gyrb genes are not clustered with previously described albicidin biosynthesis and self-protection genes. the gyra ...200818268084
effects of supplement type and selenium source on measures of growth and selenium status in yearling beef steers.sugarcane molasses is a widely used animal feed by-product, but is concentrated in s (approximately 1%, dm basis) and has been shown to reduce the cu status of cattle. dietary s may also antagonize se; therefore, two 90-d studies were conducted with forage-fed, yearling steers (12 pens; 2 steers/pen for each study) to investigate the impact of molasses supplementation on measures of se status. in exp. 1, steers were assigned isonitrogenous supplements with equivalent amounts of tdn from 2 source ...200818272852
sugarcane functional genomics: gene discovery for agronomic trait development.sugarcane is a highly productive crop used for centuries as the main source of sugar and recently to produce ethanol, a renewable bio-fuel energy source. there is increased interest in this crop due to the impending need to decrease fossil fuel usage. sugarcane has a highly polyploid genome. expressed sequence tag (est) sequencing has significantly contributed to gene discovery and expression studies used to associate function with sugarcane genes. a significant amount of data exists on regulato ...200818273390
nematodes of rhynchophorus palmarum, l. (coleoptera: curculionidae), vector of the red ring disease in coconut plantations from the north of the rio de janeiro state.rhynchophorus palmarum, the palm weevil, has been reported as a pest of palms and sugarcane plants. the red ring disease is an infectious plant disease caused by nematodes. the etiological agent, bursaphelencus (rhadinaphelencus) cocophilus (nematoda: aphelenchoididae), completes its life cycle within 9 to 10 days inside the palm tree. the main symptom is a permanent wilting of the plant aerial parts. previous studies stated that b. cocophilus cohabits with other nematodes the gut of r. palmarum ...200818278512
contrasting effects of hc-pro and 2b viral suppressors from sugarcane mosaic virus and tomato aspermy cucumovirus on the accumulation of sirnas.rna silencing and suppression of silencing are host and virus interactions for defense and counter-defense. here, we explored the function effect of hc-pro encoded by sugarcane mosaic virus (scmv) on the suppression of rna silencing. sirna northern blotting assay indicated that the replication of scmv was regulated by host rna silencing machinery. co-expression assay demonstrated that the hc-pro encoded by scmv suppressed the rna silencing induced by sense rna and dsrna. transitive rna silencing ...200818280529
soderf3, a novel sugarcane ethylene responsive factor (erf), enhances salt and drought tolerance when overexpressed in tobacco plants.the molecular signals and pathways that govern biotic and abiotic stress responses in sugarcane are poorly understood. here we describe soderf3, a sugarcane (saccharum officinarum l. cv ja60-5) cdna that encodes a 201-amino acid dna-binding protein that acts as a transcriptional regulator of the ethylene responsive factor (erf) superfamily. like other erf transcription factors, the soderf3 protein binds to the gcc box, and its deduced amino acid sequence contains an n-terminal putative nuclear l ...200818281696
genetic variability of genome segments 3 and 9 of fiji disease virus field isolates.fiji leaf gall is an important disease of sugarcane in australia and other asia-pacific countries. the causative agent is the reovirus fiji disease virus (fdv). previous reports indicate that there is variation in pathology between virus isolates. to investigate the amount of genetic variation found in fdv, 25 field isolates from australia, papua new guinea and malaysia were analysed by partial sequencing of genome segments s3 and s9. there was up to 15% divergence in the nucleotide sequence amo ...200818299794
agrobacterium tumefaciens-mediated transformation in colletotrichum falcatum and c. acutatum.agrobacterium tumefaciens-mediated transformation (atmt) is becoming an effective system as an insertional mutagenesis tool in filamentous fungi. we developed and optimized atmt for two colletotrichum species, c. falcatum and c. acutatum, which are the causal agents of sugarcane red rot and pepper anthracnose, respectively. a. tumefaciens strain sk1044, carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (gfp) gene, was used to transform the conidia of these two c ...200818309266
[sugar cane burning in brazil: respiratory health effects].the article aimed to update scientific literature information about respiratory health effects caused by sugarcane burning, considering the expansion of sugarcane plantations in brazil and in the state of são paulo. articles published between 1996 and 2006, which deal with the health effects of sugarcane burning and/or air pollutants originating from this burning, were discussed. these studies suggest that part of the population--especially the elderly, children and asthmatics--suffers health ef ...200818327496
sugarcane bagasse mild alkaline/oxidative pretreatment for ethanol production by alkaline recycle process.in order to decrease the alkali and water consumptions in the sugarcane bagasse alkaline/oxidative pretreatment for ethanol production, an alkaline recycle process was carried out. two recycles of naoh/h2o2 pretreatment did not decrease the pretreatment and enzymatic hydrolysis efficiencies and the consumptions of naoh and water would be saved by 26% and 40%, respectively. a simultaneous saccharification and fermentation (ssf) culture with pretreated bagasse as substrate was developed giving 25 ...200818327542
variation in acquisition of fiji disease virus by perkinsiella saccharicida (hemiptera: delphacidae).fiji leaf gall, caused the fiji disease virus (genus fijivirus, family reoviridae, fdv), is a serious disease of sugarcane, saccharum officinarum l., in australia and several other asia-pacific countries. in australia fdv is transmitted only by the planthopper perkinsiella saccharicida kirkaldy (hemiptera: delphacidae), in a propagative manner. successful transmission of fdv by single planthoppers confined to individual virus free plants is highly variable, even under controlled conditions. the ...200818330111
comparison of pretreatment strategies of sugarcane baggase: experimental design for citric acid production.solid state fermentation was carried out to compare efficiency of acid, alkaline and urea pretreatment of sugarcane bagasse for production of citric acid using aspergillus niger atcc 9142. plackett-burman statistical design was used to evaluate significance of variables. pretreatment of bagasse by urea was known as the most influential treatment to increase citric acid production (137.6g/kg of dry sugarcane bagasse and citric acid yield of 96% based on sugar consumed). finally, up scaling was ac ...200818334291
protein expression profile of gluconacetobacter diazotrophicus pal5, a sugarcane endophytic plant growth-promoting bacterium.this is the first broad proteomic description of gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. proteomic coverage of g. diazotrophicus pal5 was obtained by two independent approaches: 2-de followed by maldi-tof or tof-tof ms and 1-de followed by chromatography in a c18 column online coupled to an esi-q-tof or esi-it mass spectrometer. the 583 identified proteins were sorted into fu ...200818340630
comparison of three methods of parasitoid polydnavirus genomic dna isolation to facilitate polydnavirus genomic sequencing.a major long-term goal of polydnavirus (pdv) genome research is to identify novel virally encoded molecules that may serve as biopesticides to target insect pests that threaten agriculture and human health. as pdv viral replication in cell culture in vitro has not yet been achieved, several thousands of wasps must be dissected to yield enough viral dna from the adult ovaries to carry out pdv genomic sequencing. this study compares three methods of pdv genomic dna isolation for the pdv of cotesia ...200818348210
relationships between chemical properties of larval media and development of two stomoxys species (diptera: muscidae) from reunion island.the development of two cattle pests, stomoxys calcitrans l. and stomoxys niger niger macquart (diptera: muscidae), was studied in the laboratory using seven potential larval media from a dairy farm on reunion island. the media were six types of cattle feed and an old manure medium. egg-to-adult survival, duration of development, and adult live weight at emergence were determined for both fly species on each medium. the media were analyzed for ph, nitrogen, organic matter, and structural compound ...200818348795
effects of soybean proteinase inhibitors on development of the soil mite scheloribates praeincisus (acari: oribatida).proteinase inhibitors (pi) are present in plant tissues, especially in seeds, and act as a defense mechanism against herbivores and pathogens. serine pi from soybean such as bowman-birk (bbpi) and kunitz have been used to enhance resistance of sugarcane varieties to the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae), the major pest of this crop. the use of these genetically-modified plants (gm) expressing pi requires knowledge of its sustainability and environmental sa ...200818357504
the genomic sequence and biological properties of pennisetum mosaic virus, a novel monocot-infecting potyvirus.the complete nucleotide sequences of two isolates of pennisetum mosaic virus (penmv) were determined. the viral genome comprised 9,611 nucleotides (nt) excluding the 3'-terminal poly(a) sequence, with the capacity of encoding a single polyprotein of 3,065 amino acids. the large open reading frame is flanked by a 172-nt 5'-untranslated region (utr) and a 244-nt 3'-utr. sequence comparisons and phylogenetic analyses of the complete genome and polyproteins suggest that penmv is closely related to o ...200818365128
influence of mahanarva fimbriolata (stål) (hemiptera: cercopidae) injury on the quality of cane juice.mahanarva fimbriolata (stål) is an important pest in latin america and causes significant reduction in sugarcane productivity. there is no information regarding the effect of this pest on the quality of cane juice used for sugar and alcohol production. this work aimed at evaluating the quality of sugarcane juice from plants attacked by spittlebugs. the experiment was arranged in a completely randomized design with 15 replications, and comprised two treatments: control and chemical treatment with ...201318368252
detection and quantification of root-knot nematode (meloidogyne javanica), lesion nematode (pratylenchus zeae) and dagger nematode (xiphinema elongatum) parasites of sugarcane using real-time pcr.a number of different plant parasitic nematode species are found associated with sugarcane in south africa. of these, the root-knot nematode (meloidogyne javanica), the lesion nematode (pratylenchus zeae) and the dagger nematode (xiphinema elongatum) are potentially the most damaging pests. identification and enumeration of the number of these nematodes are necessary for providing advice to farmers as well as studying the effects of various treatments in field and glasshouse trials. we report on ...200818378423
differential expression analysis by cdna-aflp of saccharum spp. after inoculation with the host pathogen sporisorium scitamineum.a differential expression study on the saccharum spp.-sporisorium scitamineum pathogenic interaction was carried out in the susceptible ja60-5 and the resistant m31/45 genotypes. using cdna-aflp analysis, a total of 64 transcript-derived fragments (tdfs) was found to be differentially expressed, with the majority (67.2%) of the differential tdfs up-regulated in the resistant m31/45 cultivar. the plant response against s. scitamineum infection was complex, representing major genes involved in oxi ...200818379790
genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants.the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ...200818383223
batch fermentation model of propionic acid production by propionibacterium acidipropionici in different carbon sources.propionic acid (pa) is widely used as additive in animal feed and also in the manufacturing of cellulose-based plastics, herbicides, and perfumes. salts of propionic acid are used as preservative in food. pa is mainly produced by chemical synthesis. nowadays, pa production by fermentation of low-cost industrial wastes or renewable sources has been an interesting alternative. in the present investigation, pa production by propionibacterium acidipropionici atcc 4965 was studied using a basal mediu ...200818386184
a comparison between lime and alkaline hydrogen peroxide pretreatments of sugarcane bagasse for ethanol production.pretreatment procedures of sugarcane bagasse with lime (calcium hydroxide) or alkaline hydrogen peroxide were evaluated and compared. analyses were performed using 2(3) factorial designs, with pretreatment time, temperature, and lime loading and hydrogen peroxide concentration as factors. the responses evaluated were the yield of total reducing sugars (trs) and glucose released from pretreated bagasse after enzymatic hydrolysis. experiments were performed using the bagasse, as it comes from an a ...200818404254
effects of entomopathogenic bacterium photorhabdus temperata infection on the intestinal microbiota of the sugarcane stalk borer diatraea saccharalis (lepidoptera: crambidae).photorhabdus temperata is an entomopathogenic bacterium that is associated with nematodes of the heterorhabditidae family in a symbiotic relationship. this study investigated the effects of p. temperata infection on the intestinal microbiota of the sugarcane stalk borer diatraea saccharalis. histopathology of the infection was also investigated using scanning electron microscopy. groups of 20 larvae were infected by injection of approximately 50 bacterial cells directly into the hemocoel. after ...200818406423
oxidation in acidic medium of lignins from agricultural residues.agricultural residues as sugarcane straw and bagasse are burned in boilers for generation of energy in sugar and alcohol industries. however, excess of those by-products could be used to obtain products with higher value. pulping process generates cellulosic pulps and lignin. the lignin could be oxidized and applied in effluent treatments for heavy metal removal. oxidized lignin presents very strong chelating properties. lignins from sugarcane straw and bagasse were obtained by ethanol-water pul ...200818418748
analysis of kinetic and operational parameters in a structured model for acrylic acid production through experimental design.in biotechnological processes, a great number of factors can influence the income productivity and conversion. normally, it is not evident which of these factors are the most important and how they interact. in this work, multivariate analysis techniques are used as experimental design coupled to a detailed deterministic model to identify the parameters with the most significant impact on the model to represent well the acrylic acid production process. it is proposed as an alternative process, h ...200818418750
characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane.sixty-three sugarcane leaf samples were collected from fifty-eight sugarcane varieties, evolved from eleven major sugarcane growing states in india, australia, south africa and usa. in rt-pcr, using gene specific primers for sugarcane streak mosaic virus (scsmv)-cp, 58 of 63 sugarcane samples were found positive to the virus infection and rest of the five samples were negative. partial cp gene sequences of 42 scsmv isolates including an isolate from aphid colony (melanaphis indosacchari) infeste ...200818427969
viruses causing mosaic disease in sugarcane and their genetic diversity in southern china.a survey of cultivated hybrid sugarcane (saccharum inter-specific hybrid) and noble sugarcane (saccharum officinarum) in southern china for the presence of sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) was conducted by rt-pcr from the years 2003 to 2006. scmv and srmv, but not scsmv, were found. a high incidence of scmv and srmv coinfection was revealed in both hybrid and noble sugarcanes. all coinfected plants showed mosaic symptom, whereas ...200818438601
assessment of gut bacteria for a paratransgenic approach to control dermolepida albohirtum larvae.bacteria from the hindguts of dermolepida albohirtum larvae were assessed for their potential to be used in paratransgenic strategies that target scarab pests of sugarcane. bacteria isolated in pure culture from the hindguts of d. albohirtum larvae were from the proteobacteria, firmicutes, and actinobacteria phyla and matched closely with taxa from intestinal and rhizosphere environments. however, these isolates were not the most common gut-associated bacteria identified in denaturing gradient g ...200818456847
predicting economic losses from the continued spread of the mexican rice borer (lepidoptera: crambidae).the mexican rice borer, eoreuma loftini (dyar) (lepidoptera: crambidae), is an invasive species that originated from mexico, and it is threatening to cause major economic losses to sugarcane, saccharum spp., and rice, oryza sativa l., industries in louisiana. the insect is expected to reach sugarcane and rice production areas in louisiana by 2008, and infest all of louisiana sugarcane and rice industries by 2035. when all sugarcane in louisiana becomes infested, annual yield losses of $220 milli ...200818459384
effectiveness of attract-and-kill systems using methyl eugenol incorporated with neonicotinoid insecticides against the oriental fruit fly (diptera: tephritidae).laboratory bioassays and field trials were conducted to evaluate an "attract-and-kill" system using methyl eugenol (me) with neonicotinoid insecticides against male oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). in laboratory bioassays, mortality of male flies resulting from the conventional toxicant, naled was 98.3-100% at 24 through 72 h after treatment, whereas the neonicotinoid insecticides imidacloprid and acetamiprid caused only approximately 60-80% at 24 through ...200818459398
allele frequency of resistance to bacillus thuringiensis cry1ab corn in louisiana populations of sugarcane borer (lepidoptera: crambidae).transgenic bacillus thuringiensis (bt) corn, zea mays l., has been widely used to manage a corn borer complex in the mid-southern region of the united states. the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), has become a dominant cornstalk boring species in some areas of this region, especially in louisiana. therefore, management of sugarcane borer resistance to bt corn is critical to ensure the long-term sustainability of bt corn for the region. this study screened 280 t ...200818459416
[climatic aspects in hantavirus transmission areas in são paulo state, brazil].the objective of this study was to investigate the spatial distribution of reported hantavirus cases in são paulo state, brazil (n = 80), from 1993 to 2005 and identify local climatic patterns during this period. kernel point estimation of density was used to show the highest concentrations in ribeirão preto, são carlos, franca, tupi paulista, and greater são paulo. increase in the number of cases during this period suggests disease dissemination even when considering increased diagnostic capaci ...200818461243
growth at elevated co(2) delays the adverse effects of drought stress on leaf photosynthesis of the c(4) sugarcane.sugarcane (saccharum officinarum l. cv. cp72-2086) was grown in sunlit greenhouses at daytime [co(2)] of 360 (ambient) and 720 (elevated)mumolmol(-1). drought stress was imposed for 13d when plants were 4 months old, and various photosynthetic parameters and levels of nonstructural carbohydrates were determined for uppermost fully expanded leaves of well-watered (control) and drought stress plants. control plants at elevated [co(2)] were 34% and 25% lower in leaf stomatal conductance (g(s)) and ...200918462832
using genomic slot blot hybridization to assess intergeneric saccharum x erianthus hybrids (andropogoneae - saccharinae).the use of genomic slot blot hybridization enabled the differentiation of hybrids from selfs in saccharum x erianthus intergeneric crosses in which saccharum was used as the female parent. based on the genomic in situ hybridization technique, slot blots of dna from the parents and the progeny were blocked with the saccharum parent dna and hybridized with the labelled male erianthus genomic dna. this technique allowed a rapid screening for hybrids and was sensitive enough to detect a 1/20 dilutio ...199718464838
a molecular approach to unraveling the genetics of sugarcane, a complex polyploid of the andropogoneae tribe.modern sugarcane varieties are complex aneuploids and typically have chromosome numbers in the 100-125 range with about 5-10% of them contributed by wild relatives, mainly saccharum spontaneum, and the rest by s. officinarum. this particular genomic constitution was found favorable for mapping the s. spontaneum genome, using maize as a diploid reference for comparison. we conducted an analysis of 32 individuals derived from the selfing of variety sp 701006 using four isozymes and 53 maize probes ...199418470072
the brazilian biofuels industry.ethanol is a biofuel that is used as a replacement for approximately 3% of the fossil-based gasoline consumed in the world today. most of this biofuel is produced from sugarcane in brazil and corn in the united states. we present here the rationale for the ethanol program in brazil, its present 'status' and its perspectives. the environmental benefits of the program, particularly the contribution of ethanol to reducing the emission of greenhouse gases, are discussed, as well as the limitations t ...200818471272
microbial utilization and biopolyester synthesis of bagasse hydrolysates.cellulosic biomass is a potentially inexpensive renewable feedstock for the biorefineries of fuels, chemicals and materials. sugarcane bagasse was pretreated in dilute acid solution under moderately severe conditions, releasing sugars and other hydrolysates including volatile organic acids, furfurals and acid soluble lignin. utilization of the hydrolysates by an aerobic bacterium, ralstonia eutropha, was investigated to determine if the organic inhibitors can be removed for potential recycling a ...200818474421
efficacy of organophosphorus derivatives containing chalcones/chalcone semicarbazones against fungal pathogens of sugarcane.ten newly synthesized organophosphorus derivatives containing substituted chalcones and substituted chalcone semicarbazones were tested for their antifungal efficacy against colletotrichum falcatum, fusarium oxysporum, curvularia pallescens (all sugarcane pathogens). the o,o-diethylphosphate derivatives containing 2-chlorochalcone and 2-chlorochalcone semicarbazone exhibited 70-85% mycelial inhibition against all the test fungi at 1000 ppm. the screening results were correlated with structural f ...200218476009
enzymatic hydrolysis optimization to ethanol production by simultaneous saccharification and fermentation.there is tremendous interest in using agro-industrial wastes, such as cellulignin, as starting materials for the production of fuels and chemicals. cellulignin are the solids, which result from the acid hydrolysis of the sugarcane bagasse. the objective of this work was to optimize the enzymatic hydrolysis of the cellulose fraction of cellulignin, and to study its fermentation to ethanol using saccharomyces cerevisiae. cellulose conversion was optimized using response surface methods with ph, en ...200718478383
dilute sulfuric acid pretreatment of agricultural and agro-industrial residues for ethanol production.the potential of dilute-acid prehydrolysis as a pretreatment method for sugarcane bagasse, rice hulls, peanut shells, and cassava stalks was investigated. the prehydrolysis was performed at 122 degrees c during 20, 40, or 60 min using 2% h(2)so(4) at a solid-to-liquid ratio of 1:10. sugar formation increased with increasing reaction time. xylose, glucose, arabinose, and galactose were detected in all of the prehydrolysates, whereas mannose was found only in the prehydrolysates of peanut shells a ...200718478400
carboxymethylcellulose obtained by ethanol/water organosolv process under acid conditions.sugar cane bagasse pulps were obtained by ethanol/water organosolv process under acid and alkaline conditions. the best condition of acid pulping for the sugarcane bagasse was 0.02 mol/l sulfuric acid at 160 degrees c, for 1 h, whereas the best condition for alkaline pulping was 5% sodium hydroxide (base pulp) at 160 degrees c, for 3 h. for the residual lignin removal, the acid and alkaline pulps were submitted to a chemical bleaching using sodium chlorite. pulps under acid and alkaline conditio ...200718478417
estimation of temperature dependent parameters of a batch alcoholic fermentation process.in this work, a procedure was established to develop a mathematical model considering the effect of temperature on reaction kinetics. experiments were performed in batch mode in temperatures from 30 to 38 degrees c. the microorganism used was saccharomyces cerevisiae and the culture media, sugarcane molasses. the objective is to assess the difficulty in updating the kinetic parameters when there are changes in fermentation conditions. we conclude that, although the re-estimation is a time-consum ...200718478432
enzymatic degradation of anthracene by the white rot fungus phanerochaete chrysosporium immobilized on sugarcane bagasse.bagasse is a by-product of sugar milling and important fuel resource for that industry. it is a fibrous, low density material with a very wide range of particle sizes and high moisture content. the goal of this study is the development of a system based on the use of the ligninolytic enzyme manganese peroxidase (mnp) produced by phanerochaete chrysosporium for the degradation of polycyclic aromatic hydrocarbons (pahs), of which anthracene was selected as an example. the white rot fungus p. chrys ...200918482797
radium-226 in sugar cane, saccharum officinarum, products in the state of pernambuco, brazil.the objective of this study was to determine the 226ra concentrations in samples of sugar cane juice, which is sold for human consumption in the city of recife, capital of the state of pernambuco, brazil. the sample collections were carried out in seventeen commercial establishments where high quantities of the juice are usually sold. the methodology used to determine the concentrations of 226ra in the juice was based on 222rn emanation classical technique. concentrations of 226ra in the samples ...200818500415
extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by kluyveromyces marxianus nrrl y-7571.production of inulinase by solid state fermentation always involves an extraction step, which dictates enzyme recovery yield and is related to cultivation conditions and control of process parameters. this work is focused on the study of extraction conditions aiming to maximize yield of an inulinase obtained by solid state fermentation of sugar cane bagasse and kluyveromyces marxianus nrrl y-7571. kinetics of extraction was followed varying the kind of solvent used. after determining the best so ...200818500581
the effectiveness of four organic matter amendments for decreasing resin-extractable cr(vi) in cr(vi)-contaminated soils.this paper compared the effectiveness of four organic materials for decreasing the amounts of soil extractable cr(vi) in cr(vi)-contaminated soils using the dowex m4195 resin-extraction method. organic matters were added into cr(vi)-spiked soils [500 mg cr(vi)(kgsoil)(-1)] in the form of sugarcane dregs compost (scdc), cattle-dung compost (cdc), soybean meal (sbm) and rice bran (rb), in the amounts of 0, 1%, and 2% by dry weight, respectively. the results indicated that adding only 1% organic ma ...200918524481
expansion of sugarcane ethanol production in brazil: environmental and social challenges.several geopolitical factors, aggravated by worries of global warming, have been fueling the search for and production of renewable energy worldwide for the past few years. such demand for renewable energy is likely to benefit the sugarcane ethanol industry in brazil, not only because sugarcane ethanol has a positive energetic balance and relatively low production costs, but also because brazilian ethanol has been successfully produced and used as biofuel in the country since the 1970s. however, ...200818536250
effect of nutritional factors on cellulase enzyme and microbial protein production by aspergillus terreus and its evaluation.the biomass yield, cellulolytic activity, and protein recovery using aspergillus terreus gn1 with alkali-treated sugarcane bagasse was studied using different levels (250-600 mg of n/l of broth) of organic and inorganic nitrogen sources. e.g., cattle urine, urea, cornsteep liquor, ammonium sulfate, ammonium nitrate, ammonium iron sulfate, ammonium chloride, and sodium nitrate. among different levels of alkali-treated bagasse substrate concentrations (0.5-4.0% w/v) tested, 1.0% substrate yielded ...198218546104
enzymic saccharification of sugarcane bagasse pretreated by autohydrolysis-steam explosion.pretreatment of bagasse by autohydrolysis at 200 degrees c for 4 min and explosive defibration resulted in the solubilization of 90% of the hemicellulose (a heteroxylan) and in the production of a pulp that was highly susceptible to hydrolysis by cellulases from trichoderma reesei c-30 and qm 9414, and by a comercial preparation, meicelase. saccharification yields of 50% resulted after 24 h at 50 degrees c (ph 5.0) in enzymic digests containing 10% (w/v) bagasse pulps and 20 filter paper cellula ...198318548636
a generalized kinetic model for the study of microbial growth.the following general equation is proposed to represent the kinetics of microbial growth \documentclass{article}\pagestyle{empty}\begin{document}$$\phi (dr/dt) + \psi r + x = 0$$\end{document}, where phi and psi depend on several parameters of the fermenting system. the values of phi and psi were calculated based on results obtained in a batch lactic acid fermentation, a batch cultivation of yeast on diesel oil, and a continuous cultivation of yeast on sugarcane molasses.198318548639
effect of pretreatment on the hydrolysis of cellulose by penicillium funiculosum cellulase and recovery of enzyme.penicillium funiculosum produces a complete cellulase which brings about 97% hydrolysis of cotton and has high beta-glucosidase, xylanase, laminarinase, and lichenase activities. this article deals with the effect of different pretreatments on the hydrolysis of sugarcane bagasse by p. funiculosum enzymes and the recovery of enzyme from the insoluble residues. enzymic saccharification of bagasse pretreated with hot 1n naoh followed by neutralization with hci and steam treated under pressure (7 kg ...198318551487
fed-batch cultivation of cellulomonas on sugarcane bagasse pith.a high biomass concentration (19.9 g/l) was obtained with the fed-batch cultivation of cellulomonas on pretreated sugarcane bagasse pith. similar results in biomass concentration, yield, and substrated consumption were obtained with the discontinuous feed of bagasses as with discontinuous feed supplemented with a partial continuous addition of salts. two or more growth phases were detected, probably caused by the differential utilization of bagasse components. an acceptably low content of bagass ...198518553644
hydrolysis of sugarcane bagasse by mycelial biomass of penicillium funiculosum. 198518553781
tolerance and compensatory response of rice to sugarcane borer (lepidoptera: crambidae) injury.a 3-yr field experiment was conducted to evaluate the tolerance and compensatory response of rice (oryza sativa l.) to injury caused by sugarcane borer, diatraea saccharalis (f.), as affected by cultivar (cocodrie, francis, and jefferson), stage of crop growth during which the injury occurred (third tiller stage, panicle differentiation stage, and heading stage), and sugarcane borer density. the proportion of rice tillers with sugarcane borer injury (leaf and leaf sheath injury and/or stem injur ...200818559187
biotechnological production of xylitol from agroindustrial residues. evaluation of bioprocesses.batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. the best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g/l/h.199818576050
astaxanthinogenesis in the yeast phaffia rhodozyma : optimization of low-cost culture media and yeast cell-wall lysis.astaxanthin is a diketo-dihydroxy-carotenoid produced by phaffia rhodozyma, a basidiomicetous yeast. a low-cost fermentation medium consisting of raw sugarcane juice and urea was developed to exploit the active sucrolytic/urelolytic enzyme apparatus inherent to the yeast. as compared to the beneficial effect of 0.1 g% urea, a ready nitrogen source, mild phosphoric pre inversion of juice sucrose to glucose and fructose, promptly fermentable carbon sources, resulted in smaller benefits. corn steep ...199718576089
invertase activity of intact cells of saccharomyces cerevisiae growing on sugarcane molasses. ii. unsteady-state continuous-culture tests.during unsteady-state continuous culture of saccharomyces cerevisiae on sugarcane blackstrap molasses, the invertase activity of the intact yeast cells oscillated. disturbances were produced by changing medium composition, air rate, impeller speed, and dilution rate. the influence of the oxygen supply rate and of the dilution rate on the invertase activity depend on the medium composition. the highest invertase activity was obtained when, after a steady-state attained using unsupplemented cultur ...198718576577
long-term effects of d-003, a mixture of high molecular weight acids from sugarcane wax, on bones of ovariectomized rats: a one year study.this study was done to determine the long-term effect of d-003 on bones of ovariectomized (ovx) rats distrib-uted in 4 groups: a false-operated and three groups of ovx rats: one treated with the vehicle and two with d-003 (5 and 250 mg/kg). d-003 significantly prevented, in a dose-dependent fashion, the trabecular bone volume (tbv), trabecular number (tbn) and trabecular thickness (tbth) reduction induced in ovx rats and the increase of trabecular separation (tbsp) osteoclast number (ocn) and os ...200818604996
establishment of cotesia flavipes (hymenoptera: braconidae) in sugarcane fields of ethiopia and origin of founding population.cotesia flavipes (cameron) (hymenoptera: braconidae) is used as a classical biological control agent against chilo partellus (swinhoe) (lepidoptera: crambidae), a serious exotic pest of cereal crops in eastern and southern africa. this parasitoid has been introduced into several african countries for the control of c. partellus in maize, zea mays l., and sorghum, sorghum bicolor (l.), but it has never been released in ethiopia. it is hypothesized that it spread into ethiopia from populations rel ...200818613566
evolutionary relationship between defensins in the poaceae family strengthened by the characterization of new sugarcane defensins.plant defensins are small (45-54 amino acids), highly basic, cysteine-rich peptides structurally related to defensins of other organisms, including insects and mammals. small putative proteins (mw < 10 kda) containing eight cysteines were screened based on the sugarcane expressed sequence tag (est) database. we selected orfs that exhibited 25-100% similarity in primary sequence with other defensins in the ncbi database and that contained eight cysteines. this similarity is sufficient for folding ...200818618271
pva-hydrogel entrapped candida guilliermondii for xylitol production from sugarcane hemicellulose hydrolysate.viable cells of candida guilliermondii were immobilized by inclusion into polyvinyl alcohol (pva) hydrogel using the freezing-thawing method. entrapment experiments were planned according to a 2(3) full factorial design, using the pva concentration (80, 100, and 120 g l(-1)), the freezing temperature (-10, -15, and -20 degrees c), and the number of freezing-thawing cycles (one, three, and five) as the independent variables, integrated with three additional tests to estimate the errors. the effec ...200918633733
coat protein of potyviruses. i. comparison of the four australian strains of sugarcane mosaic virus.as an aid to a more rational classification of the potyvirus group, we have examined the molecular weight, amino acid composition, and tryptic peptide map of the coat protein of four australian sugarcane mosaic virus strains: johnson grass (jg), sugarcane (sc), queensland blue couch grass (bc), and sabi grass (sabi). the proteins migrated as a single band in sds-polyacrylamide gel electrophoresis with molecular weights of 33,700 (sc), 34,200 (jg), 39,100 (bc), and 40,300 (sabi). the bc and sabi ...198118635056
towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses.the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ...198118635068
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