Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| developmental changes of amniotic fluid alpha-fetoprotein subfractions in early gestation. | using both concanavalin a crossed-line affinity immunoelectrophoresis and lentil lectin crossed-line affinity immunoelectrophoresis, developmental changes of alpha-fetoprotein (afp) subfractions were studied in 11 samples of human amniotic fluid obtained between 43 and 121 days of gestation. in the amniotic fluid at 43 or 48 days of gestation, only subfractions produced by the yolk sac were evident. the initial appearance of the subfractions produced by the fetal liver was first detected at 51 d ... | 1983 | 6190415 |
| application of lectin--gold complexes for electron microscopic localization of glycoconjugates on thin sections. | a method is described for the electron microscopic detection of lectin-binding sites in different cellular compartments and extracellular structures that uses thin sections from resin-embedded tissues. various lectins (ricinus communis lectin i and ii, peanut lectin, lotus tetragonolobus lectin, ulex europeus lectin i, lens culinaris lectin, helix pomatia lectin, and soybean lectin) were bound to particles of colloidal gold and used for direct staining of thin sections or glycoprotein--gold comp ... | 1983 | 6190857 |
| characteristics of suppressor factors generated from human mononuclear cells using concanavalin a and c-reactive protein. | lymphocytes from normal human donors were cultured in marbrook flasks in the presence of c-reactive protein (crp), concanavalin a (con a) or serum amyloid a protein low molecular weight component (saal) and the culture supernatants were tested for their ability to suppress the pokeweed mitogen (pwm) driven response of normal human lymphocytes. the supernatants from crp and con a stimulated lymphocytes, when added to cultures at initiation at a final concentration of 5%, suppressed the plaque for ... | 1983 | 6192956 |
| t cell hybrids that express a vh idiotope-related determinant on a glycoprotein distinct from h-2, thy-1, and lyt-1 molecules. | two mouse monoclonal antibodies to chicken immunoglobulin vh-associated idiotypes (id), cid-1 and cid-2, were used as probes for id determinants on mouse t cells. cid-1, which recognized chicken antibodies to n-acetyl glucosamine (naga), and approximately 0.4% of chicken t lymphocytes also reacted with approximately 0.2% of balb/c splenic thy-1.2+ cells. when enriched cid-1+ splenic t cells from naga-immune balb/c mice were fused with the akr thymoma bw 5147 cell line, 2 of 72 resulting hybrids, ... | 1983 | 6193222 |
| synthesis of a spore-specific surface antigen during sporulation of saccharomyces cerevisiae. | antisera raised against purified yeast ascospores caused agglutination of both ascospores and vegetative cells. a spore-specific activity was obtained by absorbing out anti-vegetative activity with vegetative cells. the anti-vegetative cell activity was directed against mannan, and was probably due to exposure of some spore coat mannan at the spore surface since concanavalin a and lentil lectin also caused agglutination of ascospores. the spore-specific activity was probably determined by a prot ... | 1983 | 6193243 |
| vil-a1, a monoclonal antibody reactive with common acute lymphatic leukemia cells. | the vil-a1 monoclonal antibody raised against reh cells reacts with common acute lymphatic leukemia (call) cells but not with normal or malignant b or t lymphocytes. it also shows no binding to normal or malignant myeloid, monocytic or erythroid cells, nor does it react with thrombocytes. the antibody is of igm class and lyses call cells very efficiently in the presence of rabbit but not human complement. immunoprecipitation experiments followed by sds-polyacrylamide gel electrophoresis under re ... | 1982 | 6193374 |
| lectin binding patterns in diffuse large cell lymphoma. | the staining reaction of a panel of lectins in paraffin embedded lymph node specimens of diffuse large cell lymphoma was studied in relation to survival. in 47 of 49 patients, varying degrees of lectin binding were observed with ricinus communis agglutinin (rca), crude extract of arachis hypogaea (c-pna), concanavalin ensiformis a (con a), triticum vulgaris a (wga) and phaseolus vulgaris a (pha). binding was either absent or only minimal with pisum sativum a (psa) and lens culinaris a (lca). two ... | 1983 | 6194878 |
| presence of an antigenic determinant common to rat ige-potentiating factor, ige-suppressive factor, and fc epsilon receptors on t and b lymphocytes. | polyclonal antibodies against purified ige-potentiating factor were prepared in guinea pigs, and mouse monoclonal antibodies were prepared against inactive ige-binding factor from a t cell hybridoma. the polyclonal antibodies and two of the monoclonal antibodies bound the ige-potentiating factor, the ige suppressive factor, and the inactive ige-binding factor. the results indicate that the three ige-binding factor molecules share a common antigenic determinant. the polyclonal antibodies and the ... | 1984 | 6197453 |
| characterization of the antigen (gp600) of heymann nephritis. | the nephritogenic brush border glycoprotein antigen (gp600) of autoimmune membranous glomerulonephropathy (heymann nephritis) isolated from crude proximal renal tubular membrane fraction (fx1a) by lens culinaris lectin affinity chromatography was characterized. the molecular weight of the antigen determined by molecular exclusion chromatography was found to be 600,000. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis it resolved into five polypeptides which were in the molecular weig ... | 1984 | 6199583 |
| distinct molecular species of human alpha-fetoprotein due to differential affinities to lectins. | resolution of human alpha-fetoprotein (afp) into four distinct molecular species was demonstrated by a combination of two affinity chromatographies with crossed-immuno-affino-electrophoresis (ciae) using concanavalin a (con a) and lens culinaris hemagglutinin (lch)-a and lch-b as affinity media. of the four afps, afp1 had no affinity for con a, lch-a, or lch-b; afp2 showed a high affinity for con a, a low affinity for lch-a, and an intermediate affinity for lch-b (or a low affinity, depending on ... | 1983 | 6200049 |
| a rapid procedure for the enrichment of undenaturated, antigenically active herpes simplex virus glycoproteins. | the usefulness of lentil lectin affinity chromatography for the rapid enrichment of hsv glycoproteins in an undenatured state for both research and clinical purposes was investigated. in order to compare the lentil lectin-binding characteristics and immunologic specificities of undenatured hsv-1 and hsv-2 glycoproteins, [35s]methionine-labelled extracts of virus-infected hep-2 cells were subjected to lentil lectin affinity chromatography. individual hsv-1 and hsv-2 glycoproteins in bound and unb ... | 1984 | 6200489 |
| cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies. | the structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit t-lymphocyte cell line have been investigated. those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. the 42,000 mol. wt molecule is similar or identical to a rabbit class i major histocompatibility complex antigen and its characterization has been reported elsewhere. three different 90,000 m ... | 1984 | 6200770 |
| studies on lectin affinities of chicken alpha-fetoprotein during ontogeny. | alpha-fetoprotein in the sera of chicken embryos (ch-afp) of the 7th, 10th, 13th, 16th, and 19th day of incubation and of one-day-old chickens were examined in crossed-immunoaffino-electrophoresis (ciae) using different lectins [concanavalin a (con a), lentil, wheat germ lectin, and soybean lectin] as ligands. differences in lectin affinities, suggesting different degrees or forms of glycosylation of ch-afp during ontogeny, were demonstrated by this method. sera of chicken embryos of the same st ... | 1984 | 6200871 |
| interferon induction in mouse spleen cells by mitogenic and nonmitogenic lectins. | twenty-two species of lectin were tested for their ability to induce interferon (ifn) in mouse spleen cells. twenty-two species of lectins representing four groups, based on competition patterns with monosaccharides, were examined for their ability to induce ifn in cultured mouse spleen cells. the lectins, all belonging to the third group (concanavalin a, succinylated concanavalin a, lens culinaris lectins type a and b, and poke weed mitogen) induced ifn mainly composed of ifn gamma. they were e ... | 1984 | 6201544 |
| purification and characterization of rana pipiens brain thy-1 glycoprotein. | the occurrence of thy-1 antigens in rana brain has been studied by the use of heterologous anti-rana brain antisera raised in rabbit and balb/c mouse (thy-1.2) and akr/j mouse (thy-1.1) strains and by monoclonal anti-mouse thy-1.1 and anti-mouse thy-1.2 antibodies with the use of quantitative absorption assays. three antigenic determinants were defined on rana brain and referred to as: 1) the rana-specific xenoantigen, 2) the rana-mouse cross-reacting xenoantigen, and 3) the thy-1.1 antigen. thy ... | 1984 | 6201547 |
| a comparison of histamine secretion from peritoneal mast cells of the rat and hamster. | functional mast cells have been obtained by peritoneal lavage of the rat and hamster. both cell types released histamine on stimulation with appropriate dilutions of anti-rat ige and anti-hamster serum. the maximum response evoked by each reagent was significantly greater for the hamster cells. the release was non-cytotoxic and was in each case blocked by the corresponding soluble antigen. the rat and hamster cells responded to concanavalin a and the lectin from lentil. phosphatidylserine (ps) p ... | 1984 | 6202354 |
| alpha-fetoprotein subfractions in germ cell tumors identified by con a or lch crossed-line affinity immunoelectrophoresis. | using concanavalin a (con a) crossed-line affinity immunoelectrophoresis and lentil lectin (lch) crossed-line affinity immunoelectrophoresis, alpha-fetoprotein (afp) subfractions were studied in sera including three sera from nude mice heterotransplanted with human yolk sac tumor of the ovary and three sera from patients with yolk sac tumor, mature solid teratoma, or immature solid teratoma of the ovary. in sera of nude mice bearing yolk sac tumor or from a patient with yolk sac tumor, subfracti ... | 1984 | 6204543 |
| variation in the core and branch carbohydrate sequences of serum glycoprotein hormone alpha subunit as determined by lectin affinity chromatography. | serum alpha subunits from patients with pituitary tumours and from normal controls were studied for their ability to bind to lens culinaris agglutinin-sepharose (lca), l-phytohaemagglutinin-agarose (l-pha) and soybean agglutinin-sepharose (sba). serum alpha subunits from normal controls which had previously been shown to bind to concanavalin a-sepharose (con a) were not retained by lca. in contrast, con a-reactive alpha subunits from patients with pituitary tumours bound specifically to lca. non ... | 1984 | 6207259 |
| production and characterization of monoclonal antibody to a melanoma specific glycoprotein. | an immunogen consisting of a 4m urea extract derived from human melanoma cells (m14), that was devoid of hla-a,b,c, hla-dr antigens and fibronectin was adsorbed to lens culinaris lectin-sepharose 4b and used to immunize mice for production of monoclonal antibody to a melanoma-specific glycoprotein. screening for hybridomas secreting antibodies to melanoma associated antigens was facilitated by use of a solid phase target antigen of chemically defined medium of melanoma cells (cdm). use of these ... | 1981 | 6208119 |
| lymphocyte recognition of lymph node high endothelium. vi. evidence of distinct structures mediating binding to high endothelial cells of lymph nodes and peyer's patches. | lymphocytes migrate from blood into lymph nodes (ln) and peyer's patches (pp) of rats specifically at segments of venules lined by high endothelium (hev). we previously identified and isolated a lymphocyte surface component termed high endothelial binding factor (hebf) that appears to be involved in lymphocyte adhesion to high endothelial cells of ln. hebf has also been isolated from thoracic duct lymph and is antigenically related to the cell surface component. soluble hebf derived from deterge ... | 1984 | 6208268 |
| amniotic fluid analysis in prenatal diagnosis of neural tube defects: a comparison between six biochemical tests supplementary to the measurement of amniotic fluid alpha-fetoprotein. | concanavalin a (con a) and lens culinaris agglutinin (lca) microheterogeneity pattern of afp (crossed affinity immunoelectrophoresis), alpha-2-macroglobulin and synaptic membrane protein d-2 (rocket immunoelectrophoresis) and qualitative (polyacrylamide gel electrophoresis) and quantitative (enzyme kinetic reaction rate) acetylcholinesterase were analysed in 87 consequtive samples from normal pregnancies and 37 abnormal samples (fetal neural tube defect or abdominal wall defect). very few false ... | 1984 | 6209039 |
| differential reactivity of alpha-fetoprotein with lectins and evaluation of its usefulness in the diagnosis of hepatocellular carcinoma. | the reactivity of alpha-fetoprotein (afp) with concanavalin a (con a) and lens culinaris agglutinin (lca) was studied by crossed immuno-affinoelectrophoresis of the serum samples of 146 patients from three groups (groups i, ii and iii). fifty-one patients with benign liver diseases were included in group i, 83 patients with hepatocellular carcinoma in group ii, and 12 patients with carcinoma metastatic to the liver from digestive organs in group iii. in group i, the percentage of con a-reactive ... | 1984 | 6209189 |
| expression of cell surface lectins on activated human lymphoid cells. | a cell surface lectin found on activated human lymphoid cells has been identified and characterized using membrane glycoprotein micelles as probes. these micelles, which are large, water-soluble aggregates, are composed of glycoproteins isolated from detergent-solubilized membranes of human b lymphoblastoid cell lines by lens culinaris hemagglutinin affinity chromatography. the micelles have an average apparent molecular weight of 4 x 10(6) estimated by gel filtration and range in diameter from ... | 1982 | 6214410 |
| ige-binding factors from mouse t lymphocytes. i. formation of ige-binding factors by stimulation with homologous ige and interferon. | incubation of normal mouse spleen cells with homologous ige resulted in the formation of soluble factors that inhibited rosette formation of mouse fc epsilon r+ cells with ige-coated ox erythrocytes. the soluble factors could be absorbed with mouse or rat ige coupled to sepharose and recovered from the beads by acid elution. however, the factors had no affinity for either human ige or mouse igg. the ige-binding factors were derived from t cells. production of the factors required lyt1+ t cells a ... | 1983 | 6217246 |
| [possibilities of obtaining, by passage on plantlets, reproduction forms (porospores) in strains of alternaria (dematiaceous fungi) isolated from human cutaneous lesions and having lost the possibility of fructifying. preliminary results]. | strains of alternaria (dematiaceous hyphomycetes) isolated from lesions in men or animals generally lose quickly their ability to fructify (porospores forming) after their isolation and keeping up on artificial culture mediums classicaly used in medical diagnostic laboratories. the authors, from the fact that alternaria are fungi which are normally parasites of living plants, put, on plantlets from germinating corn seeds (graminaceous plant) and lentil seeds (papilionaceous plant), two strains o ... | 1983 | 6224543 |
| modulation of the biologic activities of ige-binding factors. iii. switching of a t cell hybrid clone from the formation of ige-suppressive factor to the formation of ige-potentiating factor. | incubation of rat-mouse t cell hybridoma cells, 23b6, with rat immunoglobulin e (ige) results in the formation of the 15,000-dalton ige-suppressive factor and the 30,000-dalton ige-binding factor, which has neither potentiating activity nor suppressive activity on the ige response. another t cell hybridoma, 23a4 cells, produces the 30,000-dalton "inactive" ige-binding factor upon incubation with ige. both the 15,000-dalton ige-suppressive factor and the 30,000-dalton ige-binding factor lacked af ... | 1983 | 6224847 |
| biphasic changes of the immunological reactivity in the course of experimental lectin-induced arthritis of rabbits. | a single injection of lens culinaris lectin (lcl) into the knee joint cavity of non-sensitized rabbits produces an arthritis with an acute and chronic phase, lasting up to one year. the persistence of the lectin in the joint, related to the strong binding affinity of lectins to glycoproteins of connective tissue structures, and the presence of specific antibodies against lcl in the serum after the intra-articular injection make this model comparable to the antigen-induced arthritis. but in our s ... | 1983 | 6227494 |
| interaction of human platelet membrane glycoproteins with collagen and lectins. | the binding of platelets to collagen is the first step in hemostasis. we attempted three approaches for elucidation of the chemical nature of receptors of human platelets for collagen. first, we examined the effect of platelet surface alteration by chymotrypsin treatment. on increasing the concentration of chymotrypsin, collagen-induced platelet aggregation and the release reaction decreased, an in parallel with this change, remarkable decrease of membrane glycoproteins iib and v, as well as 400 ... | 1984 | 6229286 |
| culture density and age-dependent interactions of 3t3 and sv3t3 cells with immobilized and soluble lens culinaris lectin. | short term binding of both balb/c derived 3t3 cells and sv3t3 cells to 2b-sepharose coated with lens culinaris lectin (lcl) was compared with their lcl-induced agglutinability in relation to culture density and age. the cells were grown for 4 days either to low (ld) or to high density (hd); for certain experiments hd-cells were stimulated (hds) by a short trypsin treatment and/or by addition of fresh medium. hd-3t3 cells bound somewhat faster to immobilized lcl than ld-3t3 cells, although ld-3t3 ... | 2016 | 6247860 |
| fractionation and characterization of allergens extracted from eggs of schistosoma japonicum. | allergenic components were partially purified from the crude extract of eggs of schistosoma japonicum by gel filtration on sephadex g-200 and ion-exchange chromatography on deae-sephadex a-50. allergenic activity was assayed by the prausnitz-küstner type skin test with infected mouse sera and radioallergosorbent test with human ser. s. japonicum egg allergens (jeal) showed single and three allergenic peaks, by isoelectrofocusing (pi = 4.8) and polyacrylamide gel electrophoresis, respectively. th ... | 1981 | 6257614 |
| characterization of human platelet surface and intracellular membranes isolated by free flow electrophoresis. | high voltage free flow electrophoresis has been applied to the separation of human platelet membranes. after short treatment with neuraminidase at the whole cell level, three membrane vesicle subpopulations have been isolated. using a surface label (125i-labeled lens culinaris lectin), the marker enzyme nadh-cytochrome c reductase, and lipid analysis, two of the fractions have been identified as of surface origin and the other consists of intracellular membrane elements. the distribution of aden ... | 1981 | 6260785 |
| glycoprotein characteristics of the sodium channel saxitoxin-binding component from mammalian sarcolemma. | the saxitoxin-binding component of the excitable membrane sodium channel exhibits glycoprotein characteristics as evidenced by its specific interaction with various agarose-immobilized lectins. the detergent-solubilized saxitoxin-binding component interacts quantitatively with immobilized wheat germ agglutinin and concanavalin a and fractionally with immobilized lens culinaris hemagglutinin and ricinus communis agglutinin. these lectins preferentially bind n-acetylglucosamine and sialic acid (wh ... | 1981 | 6268157 |
| extracellular folate deaminase of dictyostelium discoideum. | folate deaminase released from cells of dictyostelium discoideum is heterogeneous with respect to molecular weight and stability at 60 degrees c. the most heat-stable component isoelectrofocuses in a broad band at approx. ph 6. the km value of this component for folate is approx. 7 x 10(-7)m and mr approx. 40 000. the major portion if not all of the deaminase binds to immobilized concanavalin a and lentil lectin. extracellular folate deaminase has a ph-optimum of approx. ph 6.0. this is higher t ... | 1981 | 6271253 |
| alkaline phosphodiesterase i and alkaline phosphatase i in plasma membranes of herpes simplex virus type 1 transformed hamster cells. | plasma membrane extracts from herpes simplex virus type 1 transformed hamster embryo fibroblasts were chromatographed on lens culinaris lectin coupled to sepharose (lch-sepharose) and analysed by dodecyl sulphate polyacrylamide gel electrophoresis. coomassie blue-staining revealed two major protein bands with apparent molecular weights of 125 000 and of about 75 000-90 000. in plasma membranes isolated from these tumor cells prior labeled with [3h]fucose or [3h]glucosamine these bands contained ... | 1982 | 6277377 |
| lectin affinity chromatography of sindbis and rous sarcoma virus glycopeptides and oligosaccharides. | glycopeptides and endogly cosidase-digested oligosaccharides from [3h]mannose-labeled rous sarcoma virus and sinbis virus have been fractionated by lentil lectin-sepharose and concanavalin a-agarose affinity chromatography and subsequently analyzed by biogel p-4 gel filtration. only a specific subset of the con a-bound asparaginly-oligosaccharides from he two viruses was also bound to lentil lectin, and this freaction apparently represented fucose-containing, diantennary acidic-type structures ( ... | 1982 | 6286708 |
| biochemical characterization of the tetrodotoxin binding protein from electrophorus electricus. | biochemical properties of a detergent-solubilized tetrodotoxin binding component from electrophorus electricus have been examined and compared with those found for the membrane-bound protein. the toxin binding component was solubilized with high efficiency by a variety of nonionic detergents and with lower efficiency by sodium cholate and deoxycholate. detergent-solubilized preparations bound tetrodotoxin and saxitoxin tightly and specifically, and this binding was observed to be rapidly and irr ... | 1982 | 6295460 |
| characterization of a glycoprotein alpha-galactosidase from lentil seeds (lens culinaris). | alpha-galactosidase (alpha-d-galactoside galactohydrolase, ec 3.2.1.22), an enzyme responsible for mobilizing the raffinose family of oligosaccharides in legume seeds, has been isolated from lentils (lens culinaris) and purified about 4,000-fold. the sephadex gel filtration profile showed the presence of two forms of the enzyme, alpha-galactosidase i with an apparent mr = 160,000 and alpha-galactosidase ii of mr = 40,000. enzyme ii readily aggregates to form i when any attempt is made to concent ... | 1983 | 6296089 |
| redistribution of membrane 5'-nucleotidase in rabbit peritoneal polymorphonuclear leucocytes during phagocytosis. | using a quantitative phagocytic model involving oil droplet internalisation rabbit polymorphonuclear leucocytes display selective segregation of membrane constituents during the phagocytic event. a resting cell surface membrane and fractions representing vesicle membranes and uninvolved surface membranes from the active cells have been purified by density gradient sedimentation and free flow electrophoresis. the specific activity of 5'-nucleotidase, a major neutrophil surface membrane glycoprote ... | 1983 | 6303859 |
| demonstration of 125i-labelled thrombin binding platelet proteins by use of crossed immunoelectrophoresis and autoradiography. | a possible receptor for thrombin on the platelet membrane has been identified. whole platelets were treated with 125i-labelled thrombin followed by washing of the platelets, solubilization in triton x-100, crossed immunoelectrophoresis and autoradiography. a heavily labelled antigen which migrated slightly more slowly than albumin was observed. no corresponding arc was seen on the same immunoplate when stained with coomassie brilliant blue, indicating that the antigen possessed weak antigenic pr ... | 1983 | 6307375 |
| the calcitonin receptor on t 47d breast cancer cells. evidence for glycosylation. | the glycosyl nature of the receptor for the peptide hormone calcitonin has been investigated in a human breast cancer cell line, t 47d. studies have been carried out to assess the ability of various lectins and of the antibiotic tunicamycin to inhibit specific binding of calcitonin to the cells, to reduce cross-linking of photoactive calcitonin to a macromolecular receptor component and to influence calcitonin stimulation of cyclic amp. pre-incubation of cells with low concentrations of tunicamy ... | 1983 | 6309149 |
| preparation of a highly purified surface membrane fraction from rabbit polymorphonuclear leucocytes by high-voltage free-flow electrophoresis. | a surface membrane fraction of high purity and good yield has been prepared from homogenates of rabbit peritoneal polymorphonuclear leucocytes, using a preliminary sorbitol density gradient sedimentation followed by preparative high voltage electrophoresis in a thin flowing buffer film. enrichment values for the plasma membrane marker enzyme 5'-nucleotidase and 125i-labelled lens culinaris lectin, after the latter had been applied at the whole cell level, were 18-fold and 6-fold, respectively. c ... | 1983 | 6309225 |
| isolation of equine infectious anemia virus glycoproteins. lectin affinity chromatography procedures for high avidity glycoproteins. | lectin affinity chromatography procedures were evaluated for the isolation of enveloped virus glycoproteins. the major glycoprotein of equine infectious anemia virus (eiav) bound to concanavalin a (con a)-sepharose through interactions which could not be reversed by alpha-methylglucoside, but elution could be accomplished with buffers containing guanidine hydrochloride or sodium dodecyl sulfate. these denaturants, however, also released about one-half of the con a protein from the sepharose matr ... | 1983 | 6309879 |
| reaction of beef plasma and lentil seedlings cu-amine oxidases with phenylhydrazine. | the reaction of copper amine oxidases from beef plasma and lentil seedlings with hydrazine derivatives has been studied. a 1:1 stoichiometry was always found for the irreversible binding to the dimeric proteins. the formation of the adduct does not require the presence of oxygen or copper. substrates compete with hydrazine derivatives for the binding to the enzymes. the binding of hydrazines and of substrate has different effects on the epr spectra of enzymic copper. | 1983 | 6312997 |
| solubilization and partial purification of putative calcium channels labelled with [3h]-nimodipine. | high-affinity binding sites for the potent 1,4-dihydropyridine calcium channel blocker [3h]-nimodipine were solubilized from guinea-pig skeletal muscle microsomes with digitonin and chaps [3-(3-cholamidopropyl)-dimethyl-ammonio-l-propanesulfonate]. detergent-solubilized binding sites could not be sedimented by centrifugation (50,000 x g, 4 h), passed freely through 0.2 micron nitrocellulose filters and were stable at 4 degrees c with half-lives of greater than 60 h. the solubilized 1,4-dihydropy ... | 1983 | 6314149 |
| unusual heterogeneity in the glycosylation of the g protein of the hazelhurst strain of vesicular stomatitis virus. | the asparagine-linked oligosaccharides of the g protein of the hazelhurst subtype of the new jersey serotype of vesicular stomatitis virus (vsv) have been compared with the oligosaccharides from the g protein of the well-characterized indiana serotype of vsv, with baby hamster kidney cells in monolayer culture as the host for both viruses. [3h]glucosamine- and [3h]mannose-labeled glycopeptides from the g protein of purified virus were analyzed by the combined techniques of endo-beta-n-acetylgluc ... | 1983 | 6314902 |
| 5'-nucleotidase from bovine caudate nucleus synaptic plasma membranes: specificity for substrates and cations; study of the carbohydrate moiety by glycosidases. | we studied 5'-nucleotidase in preparations of synaptic plasma membranes from bovine caudate nucleus. the best substrates for this membrane-bound enzyme were purine nucleotides, particularly 5'amp. effects of metal cations and chelating agents suggest that 5'-nucleotidase is a metalloprotein. optimal conditions for solubilization of the 5'-nucleotidase were found by using a low concentration of the zwitterionic detergent sulfobetaine 14. in contrast, another membrane-bound enzyme, acetylcholinest ... | 1984 | 6321659 |
| glycoprotein gp118 of varicella-zoster virus: purification by serial affinity chromatography. | glycoprotein gp118, one of the major glycosylated proteins specified by varicella-zoster virus, is biologically of great importance since it possesses an epitope which elicits a complement-independent neutralizing antibody response. to purify this glycoprotein from a nonidet-solubilized extract of varicella-zoster virus-infected cells, we examined its affinity to a variety of ligands, including two lectins--concanavalin a and lens culinaris, cibacron blue and heparin, and finally an immunoadsorb ... | 1984 | 6321779 |
| the evaluation of a lectin-agarose based subunit vaccine and complementary diagnostic antigen for aujeszky's disease (pseudorabies) in the pig. | eleven out of 25 pigs were immunized with a lectin--agarose based subunit vaccine for aujeszky's disease (ad). the vaccine was prepared by extracting protective antigens from a non-ionic detergent (triton-x-100) extract of ad virus-infected pk-la cells with lens culinaris agglutinin immobilized on agarose beads. two groups of 3 and 4 pigs received 2 doses of vaccine each containing 426 micrograms of adsorbed protein. two groups of 2 pigs each received 2 vaccine doses containing either 23 or 33 m ... | 1984 | 6326377 |
| purification of rabbit mammary prolactin receptor by acidic elution from a prolactin affinity column. | membrane-bound prolactin receptors from the mammary gland of 6-7-day postpartum lactating rabbits were solubilized using the zwitterionic detergent, zwittergent 3-12 (3- dodecyldimethylammonio )-1-propanesulfonate). the solubilized receptor from one rabbit was bound to an ovine prolactin-agarose affinity gel and eluted at ph 4.2. the receptor appeared as a single band upon staining sodium dodecyl sulfate-polyacrylamide gels with coomassie blue. the protein yield from one rabbit was about 8 micro ... | 1984 | 6327701 |
| purification and characterization of human t-lymphocyte-derived erythroid-potentiating activity. | the human t-lymphoblast cell line, mo, secretes a number of lymphokines, including erythroid-potentiating activity (epa), an important early regulator of erythropoiesis. we report purification of epa to homogeneity, from serum-free mo-conditioned medium. purification was accomplished by sequential concentration, ammonium sulfate precipitation, lentil lectin affinity chromatography, gel filtration, and reverse-phase high-performance liquid chromatography. epa was assayed by its ability to stimula ... | 1984 | 6332112 |
| two distinct antigenic markers for rat thymus and t cells defined by monoclonal antibodies. | rat thymus and t-cell antigens were defined by using two distinct monoclonal antibodies (r-1-3b3 and r-1-12c5). r-1-3b3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and t cells but not b cells and bone marrow cells. the antigen defined by this r-1-3b3 antibody occurred more abundantly on medullary thymocytes and peripheral t cells than on cortical thymocytes. immunochemical data showed that r-1-3b3 antibody recognized a sin ... | 1983 | 6339377 |
| lectin receptors in trypanosoma cruzi. an n-acetyl-d-glucosamine-containing surface glycoprotein specific for the trypomastigote stage. | we have investigated the interaction of three lectins, differing in their sugar specificities, with the surface of the three differentiation stages of trypanosoma cruzi. the scatchard constants for each lectin and parasite stage imply that differentiation of t. cruzi is accompanied by changes in the cell surface saccharides. trypomastigotes obtained from two different sources do not differ appreciably as to the number and affinity of binding sites for the three lectins employed, suggesting a sim ... | 1983 | 6340740 |
| adult transplantation tolerance induced by lentil lectin. iii. induction of transplantation tolerance by lentil lectin in mouse strain combinations with different h-2 disparities: tolerogenic effect of h-2d region antigens. | the survival time of skin allografts was investigated in 28 combinations of mouse strains differing at various loci, in adult recipients treated with lentil seed lectin (lca). the recipients were given 1 mg of lca daily (i.v. or i.p.) after transplantation of the skin graft. the immunosuppressive effect of lca was generally inversely related to the conventional strength of the allotransplantation reaction, i.e. the rate of rejection of allografts on untreated recipients. in the case of h-2d anti ... | 1983 | 6343508 |
| adult transplantation tolerance induced by lentil lectin. i. the fate of tolerogenic lentil seed lectin after injection into mice. | intravenous administration of lentil lectin is more efficient in the induction of transplantation tolerance to mouse skin allograft than intraperitoneal or subcutaneous administration. 131i-labelled lca is eliminated relatively rapidly from the mouse body after i.v. injection (approximately 80% of a 1 mg dose eliminated after 48 h); lca administered i.p. or s.c. is eliminated 2-3 times more slowly. the rate of elimination of lca administered i.v. is only slightly lower than that of bovine serum ... | 1983 | 6347731 |
| the mechanism of natural binding of bacteria to human lymphocyte subpopulations. | some bacteria bind naturally to human lymphocyte subpopulations. here we investigated the nature of the interacting factors" on bacterial cells and on lymphocytes. first, surface ig was not involved in the binding of b. melitensis to b cells, since capping of surface ig did not affect the binding of this bacteria. we put forward the hypothesis that the binding is due to an interaction between a protein (probably a lectin) on the lymphocyte surface and a carbohydrate on the bacteria. the followin ... | 1983 | 6348298 |
| binding of mammalian beta 2-microglobulin by glycoproteins in fish serum. | the results demonstrate the presence in cod serum of beta 2-microglobulin (beta 2m)-binding molecules. upon fractionation on sephadex g-200, the bound beta 2m appears mainly in the void volume, but also as a minor peak with the apparent size of albumin. the complexes show affinity to con a-sepharose and lens culinaris lectin-sepharose, respectively, indicating that they contain glycoproteins. because of the high molecular weight of the beta 2m-containing complexes they can be separated from unbo ... | 1983 | 6353206 |
| insulin-stimulated phosphorylation of the insulin receptor precursor. | the alpha and beta subunits of the insulin receptor, mr = 135k and 95k, appear to be synthesized via a single polypeptide precursor of mr = 190k. we have investigated whether insulin stimulates the phosphorylation of this proreceptor, as is the case with mature receptor. rat liver endoplasmic reticulum membranes were solubilized in triton x-100 and chromatographed sequentially on wheat-germ agglutinin-agarose and lentil lectin-agarose columns. phosphorylation of the lentil eluate with [gamma 32p ... | 1983 | 6360168 |
| changes in nitrogen fractions and proteolytic activities in the cotyledons of germinating lentils. | changes in ninhydrin positive material, free amino acids and protein content during germination of seeds of lens culinaris med. have been studied. ninhydrin positive material and free amino acids reached their highest concentration at the fifth day of germination. total protein which represents 21% of the total dry weight of the lentil cotyledons, suffers a degradation of only 24% in seven days of germination; in the same period of time, reserve proteins underwent a degradation of 69%, legumin b ... | 1983 | 6361930 |
| purification and characterization of two proteolytic enzymes in the cotyledons of germinating lentils. | two proteases, one peptidehydrolase and one aminopeptidase, have been purified to homogeneity from cotyledons of germinating seeds of lens culinaris med. peptidehydrolase has an apparent molecular weight of 89,000 and an isoelectric point of 4.7. peptidehydrolase activity was not affected by metal chelators but it was affected by n-bromosuccinimide, phenylmethylsulfonyl fluoride and n-ethylmaleimide, suggesting the presence of tryptophan and serine residues together with free--sh groups in its a ... | 1983 | 6361931 |
| fetal forms of oligoaminopeptidase, dipeptidylaminopeptidase iv, and sucrase in human intestine and meconium. | particles of meconium sedimenting at 105,000 g contain sucrase and various brush border peptidase activities. oligoaminopeptidase, dipeptidylaminopeptidase, and sucrase solubilized by papain from meconium particles of preterm newborns or from brush border of human fetuses during the 4th month of gestation were compared with the same enzymes prepared from adult jejunal and ileal brush border. the following are characteristics of fetal intestinal brush border enzymes: (a) a faster anodal electroph ... | 1984 | 6363665 |
| characterization of a membrane antigen from rabbit testis and sperm isolated by using monoclonal antibodies and effect of its antiserum on fertility. | an antigen was isolated from deoxycholate-solubilized rabbit testis and sperm by using an immunosorbent column containing igg from a monoclonal antibody (8c10.5) that inhibits fertility. elution was by stepwise increases in ph (8.0, 10.0, and 11.4), with the ph 11.4 fraction after recycling through the column showing a single band at 63 kilodaltons in slab nadodso4/page with a silver stain. the antigen molecule was composed of two subunits, which on two-dimensional page showed many spots within ... | 1984 | 6366792 |
| light and electron microscope analysis of lectin binding to adult rat liver in situ. | a comprehensive mapping of lectin receptors on adult rat liver in situ was performed at light and ultrastructural levels by using 12 biotin-labeled lectins and an avidin-biotin-peroxidase complex. in addition, concanavalin a conjugated directly to peroxidase was utilized to study intracellular membrane glycoconjugates. to achieve optimal preservation of these membrane sugar moieties, several fixatives and fixation procedures were evaluated. a periodate-lysin-paraformaldehyde combination provided ... | 1984 | 6368972 |
| presence of hla-d/dr antigens on the membrane of breast tumour cells. | hla-d/dr (ia) glycoproteins were identified in human breast carcinoma and normal mammary gland cells by means of an anti-ia monoclonal antibody. two techniques were used: (1) immunoperoxidase staining performed on histological sections and (2) ia glycoproteins were isolated as follows: firstly by radioactive labelling of isolated cells, then by filtration on sephadex g25, followed by lens culinaris chromatography, and immune complex formation and then elution on protein a-sepharose. lastly, the ... | 1984 | 6370519 |
| the effect of lentil lectin treatment on the survival of rat skin allografts in strain combinations with different genetic disparity. | the effect of lentil lectin on the survival of skin allografts was investigated in six rat strain combinations with different genetic disparities between the donors and recipients. lca was administered i.p. (50 mg/kg) to the recipients daily, starting with the day of transplantation (day 0) until graft rejection, except that the doses on days 1, 3, 5, 7, and 9 were given i.v. (25 mg/kg). lca treatment was more efficient in prolongation of skin graft survival time in congenic strain combinations ... | 1984 | 6373390 |
| immunopharmacologic profiles of a thiol protease inhibitor, l-trans-dicyclohexyl epoxysuccinate. | ep-1, l-trans-dicyclohexyl epoxysuccinate, is a synthetic and specific inhibitor of thiol proteases. the effects of this inhibitor on some immunological parameters were examined in normal and immunity-impaired mice and rats. in the cultures of splenocytes obtained from the mice treated with ep-1, it enhanced the lymphocyte blast transformation induced by both suboptimal and optimal concentrations of concanavalin a (con a) and lens culinaris (lc). the in vivo administration of ep-1 caused a depre ... | 1984 | 6374218 |
| distinct cytoskeletal domains revealed in sperm cells. | antibodies against different cytoskeletal proteins were used to study the cytoskeletal organization of human spermatozoa. a positive staining with actin antibodies was seen in both the acrosomal cap region and the principal piece region of the tail. however, no staining was obtained with nitrobenzoxadiazol-phallacidin, suggesting that most of the actin was in the nonpolymerized form. most of the myosin immunoreactivity was confirmed to a narrow band in the neck region of spermatozoa. tubulin was ... | 1984 | 6381503 |
| dna binding proteins in canine sera. a method for removal of nonspecific dna binding in the farr assay. | a panel of canine sera, the majority of which were collected from clinically healthy dogs, were investigated for antibodies against double stranded (dsdna) by the farr radioimmunoassay technique. non-specific dna binding agents interfering with the farr assay were detected in all sera. heat inactivation at 60 degrees c or treatment with dextran sulphate was shown to eliminate this kind of unspecific dna binding while not affecting true antibodies to dsdna. canine sera positive in the farr assay ... | 1984 | 6388143 |
| d6.1, a murine antimelanoma monoclonal antibody from congenitally athymic nude mice immunized with purified melanoma tumor-associated antigen. | an igm monoclonal antibody, d6.1, has been obtained by fusion of p3 x 63ag8.653 with spleen cells from congenitally athymic nu/nu mice immunized with partially purified human melanoma taa bound to insolubilized lens culinaris lectin. the d6.1-defined antigen is present on the cell membrane of melanoma cells maintained in tissue culture but is not detected in spent culture media. eighteen of twenty-three melanoma cell lines tested were found to react with d6.1, in contrast to only 17% of nonmelan ... | 1984 | 6396196 |
| biosynthesis and glycosylation of the insulin receptor. evidence for a single polypeptide precursor of the two major subunits. | the biosynthesis and carbohydrate processing of the insulin receptor were studied in cultured human lymphocytes by means of metabolic and cell surface labeling, immunoprecipitation with anti-receptor autoantibodies, and analysis on sodium dodecyl sulfate-polyacrylamide gels under reducing conditions. in addition to the two major subunits of mr = 135,000 and mr = 95,000, two higher molecular weight bands were detected of mr = 210,000 and mr = 190,000. the mr = 210,000 band and the two major subun ... | 1983 | 6411700 |
| identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. | human blood mononuclear leukocytes stimulated with toxoplasma antigen, concanavalin a, mezerein plus lentil lectin, or staphylococcal enterotoxin a secreted a factor (macrophage-activating factor, or maf) that enhanced the capacity of human macrophages to release h2o2 and to kill toxoplasmas. the same lymphoid supernatants contained ifn gamma but not ifn alpha or ifn beta. the maf activity of six of seven unfractionated supernatants was completely eliminated by a monoclonal antibody that neutral ... | 1983 | 6411853 |
| influence of various mitogens on the yield of sister-chromatid exchanges, induced by chemicals, in human lymphocytes. | the fluorochrome-plus-giemsa (fpg) method of perry and wolff was used to compare the frequencies of sister-chromatid exchanges (sces) induced by cyclophosphamide (cp) or mitomycin c (mmc) in human lymphocytes stimulated by phytohaemagglutinin (pha), concanavalin a (con a), wistaria floribunda (wfa), or lentil lectin (lch-a) extracts. these 4 mitogens, differing in lectin valency and/or sugar specificity, are considered as activating primarily thymus-derived (t) lymphocytes. regardless of the mit ... | 1983 | 6415475 |
| pregnancy-related changes in the mouse oviduct and uterus revealed by differential binding of fluoresceinated lectins. | the binding of 20 fluorescein isothiocyanate (fitc)-labeled lectins to various portions of the pregnant and non-pregnant murine oviduct and uterus was studied by fluorescence microscopy. five lectins (from ricinus communis (rca-i), maclura pomifera (mpa), triticum vulgare (wheat germ-wga), bauhinia purpurea (bpa), and ulex europeus (uea-i] reacted differentially with the epithelium of pregnant as compared with the non-pregnant uterus. the binding of rca-i, mpa and wga delineated pregnancy-relate ... | 1983 | 6418694 |
| carbohydrates of influenza virus. v. oligosaccharides attached to individual glycosylation sites of the hemagglutinin of fowl plague virus. | the carbohydrate side chains of the hemagglutinin of fowl plague virus (a/fpv/rostock/34 (h7n1] have been localized by a procedure involving fragmentation of the polypeptide with cyanogen bromide and various proteases. the positions of the fragments were determined by radioactive labeling of the sugars and of specific amino acids. side chains of the complex type i are attached to asparagine residues 12, 28, 123, 149, and 478. a mannose-rich (type ii) side chain is linked to asparagine 406. aspar ... | 1984 | 6422625 |
| antigenic similarities both inside and outside the carbohydrate-binding sites of two-chain and one-chain leguminous lectins. | antibodies were made against the two-chain lectin lath-o from the seeds of lathyrus odoratus as well as its isolated light (alpha) and heavy (beta) chains. these antibodies were used to antigenically compare the lath-o with other two-chain lectins like lath-s, lentil and vicia cracca glc specific and with one-chain lectins like con a, pha, peanut and soybean. sensitive elisa tests showed that there was a distinct antigenic cross-reaction between the one-chain and two-chain lectins both by using ... | 1984 | 6424408 |
| pepsin-generated type vi collagen is a degradation product of gp140. | a major extracellular matrix glycoprotein, gp140 , synthesized by wi-38 human lung fibroblasts has previously been shown to be collagen-like. a form of gp140 that is related to extracellular matrix gp140 both antigenically and in apparent molecular mass was isolated from human placenta. types i-vi collagen were isolated from human tissues by limited pepsin digestion, selective salt precipitation, and chromatography. immunoblot analysis of the collagens and gp140 utilizing affinity-purified polyc ... | 1984 | 6427226 |
| characterization, localization, and biosynthesis of an interstitial retinol-binding glycoprotein in the human eye. | human eyes contain an mr 135k retinol-binding protein that is analogous to interstitial retinol-binding protein ( irbp ) in the subretinal space of bovine eyes. it is a glycoprotein, because it binds 125i-concanavalin a, 125i-wheat germ agglutinin and 125i-lens culinaris hemagglutinin. it does not bind ricinus communis agglutinin i. after desialation, it binds ricinus communis agglutinin i, but loses its capacity to bind wheat germ agglutinin. these observations, coupled with the known specifici ... | 1984 | 6427409 |
| further characterization of porcine plasma fibronectin which contains fucosylated carbohydrate chains. | porcine plasma fibronectin and its functional four fragments produced by cathepsin b digestion were examined for biological, immunochemical and biochemical properties. native fibronectin, 150-kda and 130-kda fragments exhibited similar cell attachment-promoting activity to each other. in an ouchterlony double immunodiffusion system, these three polypeptides formed a precipitin line with anti-fibronectin antiserum, while the 50-kda and 30-kda fragments did not. the 150-kda and 130-kda fragments c ... | 1984 | 6428463 |
| interferon production in children with undue susceptibility to infections. | the interferon-producing ability of peripheral blood mononuclear leukocytes obtained from children with undue susceptibility to infections was investigated. patients were grouped according to three clinical patterns, i.e. children with mainly upper respiratory tract infections, with lower respiratory tract infections and with frequent middle ear infections. there was no evidence of impaired mean interferon-alpha responses to the inducers sendai virus and beta-hemolytic group g streptococci in pa ... | 1984 | 6430024 |
| correlation of interferon gamma inducing ability to sugar binding specificities of various lectins. | various lectins were examined to determine possible induction of gamma interferon (ifn-gamma) in human leukocytes. among the seven positive lectins (concanavalin a, pea lectin, lentil lectin, rice bran agglutinin, pokeweed mitogen, wheat germ agglutinin, phytohemagglutinin-p), six except rice bran agglutinin belonged to those which recognize carbohydrate chains connected to polypeptide through a glycosylamine linkage between n-acetylglucosamine (glcnac) and asparagine residues. the specificity o ... | 1984 | 6431020 |
| characterization of fibronectin from fetal human plasma in comparison with adult plasma fibronectin. | fibronectin was purified from fetal human plasma and characterized in comparison with fibronectin from adult human plasma. fetal plasma fibronectin had an amino-acid composition, immunological properties, and cell-attachment-promoting activity similar to those of adult plasma fibronectin. however, fetal plasma fibronectin was shown to have a distinct carbohydrate composition which is characterized by the presence of fucose. to ascertain the differences in the carbohydrate moiety, 14c-labeled gly ... | 1984 | 6433980 |
| isolation and characterization of human placenta fibronectin. | fibronectin was isolated from human placenta tissues and compared with human plasma fibronectin. placenta and plasma fibronectins had similar amino acid compositions, immunological properties, and cell attachment-promoting activities, but differed in apparent molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which could be accounted for at least partly by the difference in carbohydrate composition. unlike plasma fibronectin, placenta fibronectin failed to form a prec ... | 1984 | 6436230 |
| markedly enhanced production of gamma interferon in murine t lymphocytes treated with lentil lectin and the diterpene ester, mezerein. | gamma interferon (ifn-gamma) was induced in murine splenocytes first stimulated to grow by concanavalin a (con a) and subsequently treated for 3 h with the diterpene ester, mezerein (mzn) and then with lectin from lens culinaris for 24 h. yields as high as 60,000 u/ml were obtained in cells from either male or female, random-bred, white swiss mice or inbred c67b1/6 mice. antibody to thy 1.2 surface antigen completely obliterated the mouse gamma interferon (muifn-gamma) response, whereas anti-lyt ... | 1984 | 6436398 |
| lectin binding to cystic stages of taenia taeniaeformis. | studies of membrane glycoconjugates of taenia taeniaeformis were initiated by assays of the lectin binding characteristics of 35-day-old cysticerci. parasites fixed in glutaraldehyde were incubated with one of the following fitc-labelled lectins: concanavalin a (con a), lens culinaris agglutinin (lca), ricinus communis agglutinin (rca), peanut agglutinin (pna), fucose binding protein (fbp) and wheat germ agglutinin (wga) and either their specific or a nonspecific sugar. ultraviolet microscopy re ... | 1984 | 6439843 |
| investigation on extracellular slime substance produced by staphylococcus epidermidis. | the extracellular slime substance produced by staphylococcus epidermidis was investigated. slime production was assessed by bacterial agglutination in the presence of concanavalin a (con a) or poly-l-lysine and by bacterial adherence to polyethylene. media for slime production was optimized using these criteria. a phenol-saline extract of crude slime was separated into four fractions on a deae-sepharose column. total protein and sugar content and the monosaccharide constituents were determined. ... | 1984 | 6442510 |
| biochemical studies of the maturation of the small sindbis virus glycoprotein e3. | a small glycoprotein (e3) was purified from the culture fluid of sindbis virus-infected primary chick embryo fibroblasts. tryptic peptide mapping and pulse-chase studies verified that this protein was produced as a by-product of the cleavage of the precursor protein pe2 to produce the envelope glycoprotein e2. a 2600-fold purification was achieved via a procedure which used differential ethanol precipitation, gel filtration, ion-exchange chromatography, and affinity chromatography on a lentil le ... | 1984 | 6443592 |
| fractionation of human lymphocytes with plant lectins. iii. identification of cells regulating the in vitro response to l-phytohaemagglutinin. | peripheral blood lymphocytes (pbl) were separated into two subclasses by differential adherence to wheat germ agglutinin (wga). wga-adherent pbl differed structurally (different wga-binding properties) and functionally from wga-non-adherent cells. as judged by [3h]-thymidine incorporation, wga-adherent pbl responded less well to l-phytohaemagglutinin (l-pha) than non-adherent cells. this difference was not due to different l-pha dose requirements or different response kinetics. wga-adherent and ... | 1980 | 6445871 |
| isolation, purification, and partial characterization of platelet membrane glycoproteins iib and iiia. | platelet membrane glycoproteins iib and iiia were isolated and purified from human platelet membranes using lentil lectin affinity chromatography and electrophoretic elution from sodium dodecyl sulfate-polyacrylamide gels. two-dimensional immunoelectrophoresis of a mixture of the purified proteins against monospecific antisera showed antigenic uniqueness of the separate polypeptides. computerized analysis of autoradiographs of two-dimensional tryptic 125i peptide maps revealed that the two glyco ... | 1981 | 6450761 |
| regulation of ige response by ige binding factors. | t lymphocytes from rats infected with nippostrongylus brasiliensis (nb) release a soluble factor with affinity for ige that selectively potentiates the ige response to an unrelated antigen. the factor is derived from fc omega receptors on t cells, binds to surface ige on precursors of ige-forming cells, and enhances their differentiation. the factor had a molecular weight between 10,000 to 20,000 and an affinity for lentil lectin. t cells from nb-infected rats formed another ige-binding factor u ... | 1981 | 6453729 |
| age-dependent changes of the levels of suppressor and helper t cells in new zealand black mice. | peanut agglutinin-positive (pna+) t cells in concanavalin a (con a) activated spleen cells were found to markedly decrease with age in new zealand black (nzb) mice but not in non-autoimmune strains, using a fluorescence activated cell sorter (facs ii). on the other hand limulus polyphemus agglutinin positive (lpa+) t cells in lens culinaris agglutinin (lca) activated spleen cells were found to gradually decrease both in nzb and non-autoimmune strains. since pna has previously been shown to selec ... | 1980 | 6456367 |
| ige-specific suppressor factors in normal human serum. | the effect of normal human serum on in vitro ige production was studied in an attempt to determine whether ige-specific suppressor factors are present in the circulation of nonallergic individuals. sera from 10 nonatopic donors (serum ige less than 20 i.u./ml) were filtered through diaflo cf50a membranes (cutoff point 50,000 d) and various dilutions of the ige-free serum filtrates (less than 150 pg/ml of ige) were examined for their ability to suppress spontaneous in vitro ige synthesis by perip ... | 1984 | 6467688 |
| solubilization and some properties of a semicarbazide-sensitive amine oxidase in brown adipose tissue of the rat. | a semicarbazide-sensitive clorgyline-resistant amine oxidase (ssao) was solubilized from membrane fractions of rat brown adipose tissue by the non-ionic detergent, triton x-100. alteration of ionic strength or addition of chelating agents alone failed to release the enzyme from its membrane. lipid-depletion led to loss of enzyme activity and alteration of substrate affinity. over 80% of the activity of the solubilized enzyme was found in gel filtration fractions corresponding to an mr of between ... | 1984 | 6477528 |
| phagocytosis of lectin-coated beads by dystrophic and normal retinal pigment epithelium. | in the dystrophic pigmented royal college of surgeons (rcs) rat, the retinal pigment epithelium (rpe) has a diminished capacity to phagocytose shed photoreceptor outer segments (ros). an alteration in phagocytic recognition or ligand-receptor interactions between the rpe and ros's could contribute to this defect. to this end, we have examined whether or not rpe lectin receptors are implicated in phagocytosis in the normal and dystrophic rat rpe by comparing differences in phagocytic uptake of le ... | 1984 | 6479240 |
| lectin-ferritin binding on dystrophic and normal retinal pigment epithelial membranes. | phagocytosis in the rat retina is a process which involves uptake of shed photoreceptor outer segments by the overlying retinal pigment epithelium (rpe). in rats with inherited retinal degeneration, there is a defect in phagocytosis. one aspect of this phagocytic defect may be an alteration in glycoconjugate-containing membrane components on rpe membranes which mediate this phagocytic interaction. lectin binding sites have been studied in order to localize the distribution of glycoconjugates on ... | 1984 | 6481408 |
| immunosuppressive factors in ascites fluids from ovarian cancer patients. | the nonspecific immunosuppressive effect of ascites fluids from ovarian cancer patients was examined and compared with that of noncancerous abdominal effusion and sera from ovarian cancer patients. the malignant ascites fluids produced a noncytotoxic, dose-dependent suppression of dna synthesis of phytohemagglutinin-stimulated human peripheral blood lymphocytes. the suppression was higher than that observed in sera from cancer patients. no suppressive effect was seen in control abdominal effusio ... | 1984 | 6528882 |
| identification of subpopulations of human urinary plasminogen activators. | enzymes functioning as plasminogen activators in commercial urokinase preparations and individual human urine concentrates were subjected to affinity chromatography on columns of lentil lectin-sepharose, ricin-sepharose, wheat germ agglutinin-sepharose, lotus lectin-sepharose and concanavalin a-sepharose. chromatography of the enzymes from both sources yielded similar results for all lectins except lentil lectin. urokinase from several commercial sources was approximately 50% adherent to lentil ... | 1984 | 6539958 |
| flow cytometric analysis of lectin-binding to canine blood lymphocytes. | the cell surface glycoconjugates of blood lymphocytes from 19 dogs with and without neoplastic disease were quantitated using flow cytofluorometric analysis of the binding characteristics of 3 lectins, namely, wheat germ agglutinin, concanavalin-a, and lens culinaris agglutinin. the specificity of lectin binding was determined using competitive monosaccharide inhibitors. the results show enhanced binding of concanavalin-a to blood lymphocytes from dogs with lymphosarcoma relative to healthy dogs ... | 1984 | 6548849 |
| analysis of lectin receptors on rat embryo fibroblasts transformed by adenovirus 2. | transformation of rat embryo fibroblasts by human adenovirus type 2 (ad2) results in the production of a series of cell lines that cover a spectrum of malignancy from nontumorigenic to highly tumorigenic in a single species. a panel of plant lectins was used to study surface characteristics of these cell lines that might correlate with tumorigenicity. because of the complex nature of lectin-cell surface interactions, a number of parameters were determined; they included numbers and densities of ... | 1983 | 6573539 |
| characterization of l-cam, a major cell adhesion molecule from embryonic liver cells. | we have developed a method for purifying l-cam, the cell adhesion molecule from embryonic chicken liver cells, and have compared its properties with those of n-cam, the neural cell adhesion molecule. l-cam was released from membranes with trypsin, purified by a series of chemical techniques, and used to generate monoclonal antibodies which allowed the identification of the intact l-cam molecule from membranes. the monoclonal antibodies were used to isolate trypsin-released l-cam in a single step ... | 1983 | 6573655 |
| a monoclonal antibody (sj-9a4) to p24 present on common alls, neuroblastomas and platelets - ii. characterization of p24 and shedding in vitro and in vivo. | the release of soluble p24 antigen into culture medium by common acute lymphoblastic leukemia (c-all) and neuroblastoma (nb) cell lines was studied. p24 release by c-all cells was detected using a solid phase indirect radioimmunometric assay (ira) which combines the specificity of lectins and monoclonal antibodies (moab) and using immunoadsorption of labeled p24 in spent medium from cells incubated with 35s-methionine (met). no p24 was present in the medium of cells pulse labeled at 37 degrees c ... | 1983 | 6578391 |
| demonstration and partial characterization of chronic lymphocytic leukemia lymphocyte-associated antigens by crossed immunoelectrophoresis of triton x-100 extracts. | crossed immunoelectrophoresis was used to study the antigens of chronic lymphocytic leukemia lymphocytes. a reference pattern was obtained and 20 samples from 18 patients were compared with this pattern. extensive variation in the expression of individual antigens was observed. the antigens in the reference pattern were further characterized by modifications of crossed immunoelectrophoresis, viz. labelling of intact lymphocytes by lactoperoxidase-catalyzed iodination, affinity crossed immunoelec ... | 1983 | 6579822 |