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dna, rna, protein and heterochromatin changes during embryo development and germination of soybean (glycine max l.).dna, rna, protein and heterochromatin were measured cytophotometrically in developing soybean (glycine max) seeds. the average 2c dna content for the soybean genome was 2.64 pg. the amounts of nuclear dna in embryo axes showed no significant change during embryo development, whereas the dna content in cotyledon nuclei increased significantly from 3.58 pg to 5.49 pg. the number of endopolyploid nuclei increased from 26% to 48% and the dna content from 4.45 to 5.49 pg after cessation of cell divis ...19836187712
evaluation of active versus passive uptake of metabolites by rhizobium japonicum bacteroids.rhizobium japonicum bacteroids were isolated anaerobically from soybean [glycine max (l.) merr] nodules. the bacteroids, which were capable of acetylene reduction and respiration, were used to study the uptake of metabolites by a method which permits correction for nonspecific adsorption of metabolites and estimation of total cell volume. these determinations permit active uptake to be assessed from metabolite accumulation against a concentration gradient. succinate, malate, alpha-ketoglutarate, ...19846203891
purification and properties of protease f, a bacterial enzyme with chymotrypsin and elastase specificities.it has been previously demonstrated that commercial bacterial fibrinolysin (ec 3.4.21.7) selectively cleaves the bond between met-53 and ala-54 in ovine prolactin (199 amino acids). a one-step purification procedure on deae-cellulose for protease f, which is the active component of bacterial fibrinolysin, and properties of the purified enzyme are reported. the enzyme is homogeneous as judged by acrylamide gel electrophoresis. its molecular weight, calculated from gel filtration experiments on se ...19836226244
epr spectroscopy of soybean lipoxygenase-1. determination of the zero-field splitting constants of high-spin fe(iii) signals from temperature and microwave frequency dependence.the zero-field splitting constants (d) of the different components building up the high-spin fe(iii) epr spectrum of lipoxygenase from soybeans were determined by two methods: (1) temperature dependence studies using the low-spin fe(iii) signal of cytochrome c at g 3 for accurate measuring of the temperature in the sample; (2) by establishing g-shift upon increasing the microwave frequency. the ranges of d for the axial and rhombic species contributing to the complex signal at g 6 are found to b ...19806250632
organization and expression of leghaemoglobin genes.leghaemoglobin genes in soybean (glycine max) are present as a moderately reiterated family of sequences. since there are identical restriction site patterns of these sequences in dna isolated from leaf, root, or nodule tissue, the data suggest that no major changes in the organization or methylation of leghaemoglobin genes occur during their induction. cloned soybean leghaemoglobin-cdna cross hybridized with rna from root nodules of kidney bean (phaseolus vulgaris), and to a lesser extent, of p ...19826282418
interspecific plasmid and genomic dna sequence homologies and localization of nif genes in effective and ineffective strains of rhizobium japonicum.three strains of rhizobium japonicum were examined for the presence of interspecific conserved plasmid-borne dna sequences and the location of their nif dna sequences. strains 61a76 and 110, which both form effective (nitrogen fixing) nodules on soybeans show very low (24%) total dna sequence homology with each other; strain 61a76 contains one plasmid, and strain 110 contains no identifiable plasmids. strain 61a24 which forms ineffective nodules on soybeans shows relatively high (50%) sequence h ...19816286824
closely related families of genes code for the alpha and alpha' subunits of the soybean 7s storage protein complex.nineteen cloned cdnas encoding the alpha and alpha'-subunits of the 7s seed storage protein in the soybean, glycine max, have been isolated from a recombinant cdna library constructed with mrna from maturing seeds. in addition, a gene encoding an alpha'-subunit has been isolated from a recombinant charon 4a phage library containing genomic glycine max dna. the cloned dnas have been divided, on the basis of their endonuclease sites, into two main classes of sequences which differ in approximately ...19826298713
metabolic effects of dietary fiber from dehulled soybeans in humans.the normal diet of six healthy volunteers was supplemented by 21 g of dietary fiber from two different soybean seed fiber preparations, either a nonpurified and never-dried soya pulp a (39% dietary fiber) or a purified soya fiber b (79%), for 3 wk each. mean daily fecal wet weight was increased by 19 and 38% in the fiber periods a and b as compared to a 2-wk control period (p less than 0.05). stool frequency and transit time remained unchanged. fecal fiber increased by 52% only during period b. ...19836305183
ultrastructural localization of bowman-birk inhibitor on thin sections of glycine max (soybean) cv. maple arrow by the gold method.the soybean bowman-birk inhibitor (bbi), a polypeptide of mw 8,000, has a specificity directed against trypsin and chymotrypsin. bbi was localized at the ultrastructural level by the protein a gold method on thin sections of glycine max (soybean) cv. maple arrow. in cotyledon and embryonic axis, bbi was found in all protein bodies, the nucleus and, to a lesser extent, the cytoplasm. contrary to the kunitz trypsin inhibitor (horisberger and tacchini-vonlanthen 1983), bbi was not present in the ce ...19836305885
characterization of dolichol and dolichyl phosphate phosphatase from soya beans (glycine max).a series of polyprenols, ranging in length from 15 to 22 isoprene units, has been isolated from soya beans (glycine max) and purified by high-pressure liquid chromatography. n.m.r., i.r. and mass spectra of the compounds indicated that they are alpha-saturated polyprenols of the dolichol type. the amount present in dry seeds was about 9 mg/100 g, whereas dolichyl phosphate (dol-p) was present only in trace amounts. dol-p phosphatase activity was detected in the microsomal fraction of 5-day-old g ...19836311165
use of plant cell cultures to study the metabolism of environmental chemicals.the metabolism of the following environmental chemicals has been studied in cell suspension cultures of wheat (triticum aestivum l.) and soybean (glycine max l.):2, 4-dichlorophenoxyacetic acid (2,4-d), 2,4,5-trichlorophenoxyacetic acid (2,4,5-t), hexachlorobenzene, pentachlorophenol, diethylhexylphthalate , benzo [alpha] pyrene, and ddt. all chemicals tested, including the persistent ones, were partially metabolized. polar conjugates predominated in all cases. a covalent incorporation into lign ...19846325118
isolation and expression of rhizobium japonicum cloned dna encoding an early soybean nodulation function.a first visible step in the nodulation of legumes by rhizobium spp. is the deformation and curling of root hairs. we have identified and cloned dna sequences encoding this function from two strains of rhizobium japonicum (usda 122 and usda 110) with a weakly homologous probe from rhizobium meliloti. root hair curling encoded by the cloned dna fragments was examined on soybeans (glycine soja ) after conjugative transfer of these sequences in broad-host-range vectors to various bacterial genera. p ...19846327649
characterization of the binding of the tc-83 strain of venezuelan equine encephalomyelitis virus to bw-j-m, a mouse macrophage-like cell line.the first step in virus replication is attachment of the virus to the host cell surface. to investigate this process, the binding of tc-83 (the attenuated strain of venezuelan equine encephalomyelitis virus) to bw-j-m (a macrophage-like murine cell culture line) has been characterized. the binding of radiolabelled virus can be blocked by excess unlabelled virus and has a ph optimum in the physiological range. binding is saturable; analysis using scatchard plots or the computerized binding data a ...19836337232
pancreatic proteinases from man, trout, rat, pig, cow, chicken, mink and fox. enzyme activities and inhibition by soybean and lima bean proteinase inhibitors.1. the specific activities of the trypsins and chymotrypsins, measured with synthetic substrates, varied within one order of magnitude. 2. the trout trypsin and chymotrypsin were 5-15 times as efficient in hydrolyzing casein as the remaining animals. 3. the inhibition of total caseinolytic activity in extracts of pancreatic tissue by sbti and inhibitors in crude extracts of raw soybeans varied ten-fold. 4. the animals may be ranked as follows according to the sensitivity of the caseinolytic acti ...19836340948
ultrastructural localization of kunitz inhibitor on thin sections of glycine max (soybean) cv. maple arrow by the gold method.the soybean trypsin inhibitor (sbti, kunitz type) was localized by immunofluorescence and, at the ultrastructural level, by the protein a gold method on thin sections of glycine max (soybean) cv. maple arrow. sbti was localized in cell walls, protein bodies, the cytoplasm between the lipid-containing spherosomes, and the nucleus of the cotyledon and embryonic axis. in the nucleus, sbti was present in the chromatin deposit and the nucleolus. the intensity of marking by the gold method decreased i ...19836341322
protease inhibitors: possible anticarcinogens in edible seeds.protease inhibitors, which are common constituents of seeds (rice, beans, and maize) have been shown to inhibit breast, colon, and skin cancers in animal experiments. epidemiological studies have shown that diets rich in these components (ie seed proteins) decrease the occurrence of prostatic, breast, and colon cancers in man. we have demonstrated that a typical purified inhibitor from soybeans, the bowman-birk inhibitor, is excreted in the feces complexed with digestive proteases from the duode ...19836346296
lectin receptors as markers of lymphoid cells. i. demonstration in tissue section by peroxidase technique.the distribution of lectin binding receptors in human lymphoid tissue (lymph node and tonsil) was studied in an effort to identify lectins with potential application as cell markers. using a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, the binding properties of five lectins were investigated: concanavalin a (con a), arachis hypogaea (peanut agglutinin), lotus tetragonolobus (asparagus pea), glycine max (soybean agglutinin) and bandeiraea simplicifolia. thre ...19846380299
comparison of in vitro and in vivo measurements of dietary ca exchangeability and bioavailability.an in vitro method for measuring dietary mineral exchangeability (miscibility with an extrinsic isotopic tracer) was tested by comparison with in vivo measurements in rats. collards, spinach and soybeans, intrinsically labeled with 45ca, were fed to rats together with extrinsically added 47ca. absorption of both tracers was determined by measuring their concentrations in the femur 2 days after consumption of the labeled test meals. the same intrinsically and extrinsically labeled foods were dige ...19836401805
[inhibitory effect of lectins extracted from arachis hypogea and soybeans on germ cell migration in the embryo of xenopus laevis (amphibia, anura)].effect of injection of arachis hypogea (pna) and soybean (sba) lectins on the migration of xenopus primordial germ cells (pgcs) were investigated. pna inhibited almost entirely the pgcs migration, more than sba. our results indicated that the inhibition of the migration could be a consequence of the lectin-induced alterations of cell surface properties.19836412978
gossypol in cottonseed products: toxicology and inactivation.the little attention given to the potential of cottonseeds as food for monogastric animals and man is due to the presence of toxic principles in them, especially gossypol. the injury to livestock associated with ingestion of cottonseeds results from the unfavorable physiological effect of gossypol per se, as well as from its adverse effects on the protein nutritive value of the food (mainly by rendering lysine metabolically unavailable). various approaches have been employed to overcome these pr ...19836414436
differentiation of bacillus anthracis and other bacillus species by lectins.bacillus anthracis was agglutinated by several lectins, including those from griffonia simplicifolia, glycine max, abrus precatorius, and ricinus communis. some strains of bacillus cereus var. mycoides (b. mycoides) were strongly reactive with the lectin from helix pomatia and weakly reactive with the g. max lectin. the differential interactions between bacillus species and lectins afforded a means of distinguishing b. anthracis from other bacilli. b. cereus strains exhibited heterogeneity with ...19846418761
radiolabeled iron in soybeans: intrinsic labeling and bioavailability of iron to rats from defatted flour.soybeans can be efficiently labeled with radiolabeled iron by supplying the iron via a nutrient culture medium as an iron salt or as a chelate. by using dual labeled iron and edta, it was determined that none of the chelator was transported to the shoots with the iron. therefore, the use of chelated iron as the iron source in the nutrient medium should not affect assessments of bioavailability of iron from plants. bioavailability (determined from whole-body retention curves of 59fe in rats) of i ...19846427432
pentane formation during the anaerobic reactions of reticulocyte lipoxygenase. comparison with lipoxygenases from soybeans and green pea seeds.the lipoxygenases from reticulocytes, soybeans and green pea seeds produce pentane in an anaerobic assay containing 13ls-hydroperoxy-9-cis, 11-trans-octadecadienoic acid and 9,12-all-cis-octadecadienoic acid. the presence of oxygen strongly inhibits pentane formation by the three enzymes. relative to the lipoxygenase activity with linoleic acid as substrate, soybean lipoxygenase is 4-times as effective in pentane formation as the lipoxygenases from reticulocytes or green pea seeds. pentane forma ...19846433987
the formation of products containing a conjugated tetraenoic system by pure reticulocyte lipoxygenase.the pure lipoxygenase from reticulocytes converts 5,15-di-hete at 2 degrees c to product(s) showing a characteristic uv spectrum with maxima of strong absorbance at 300 and 316 nm and shoulders at 285 and 350 nm. their formation was completely prevented by the lipoxygenase inhibitors, etya and ndga, by heating of the enzyme and by anaerobiosis. at 35 degrees c the products were formed only initially and in small amounts. the reaction products were purified by sp-hplc and shown to migrate in the ...19846437870
the inactivation of lipoxygenases by acetylenic fatty acids.5,8,11,14-eicosatetraynoic acid (etya) inactivates irreversibly the lipoxygenases from soybeans and reticulocytes in a time-dependent manner. 5,8,11-eicosatriynoic acid (etrya) is a powerful inactivator only for reticulocyte lipoxygenase. several types of experimental evidence indicate that the acetylenic fatty acids act as suicidal substrates which are converted during the lipoxygenase reaction probably to allene hydroperoxides. these highly reactive intermediates or radical precursors react im ...19846440550
glucosylation of phosphorylpolyisoprenol and sterol at the plasma membrane of soya-bean (glycine max) protoplasts.protoplasts were prepared from cells of soya-bean (glycine max) suspension cultures and the plasma membrane was labelled with diazotized [g-3h]sulphanilic acid. homogenates were fractionated by differential and isopycnic centrifugation, and membrane fractions in a density gradient were characterized by enzymic markers and the radioactive label. when fractions containing a large amount of protein were incubated with udp-[u-14c]glucose, radioactive material soluble in chloroform/methanol was forme ...19806445731
a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557.the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ...19846481336
a quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as o-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries.complete gas-liquid chromatographic separation of o-methyloxime acetates (syn and anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica carbowax 20m capillary column. a single hydrolytic step for carbohydrate-containing biological material (less than or equal to 2.5 x 10(-3) m sugar solution in 4 n trifluoroacetic acid at 125 degrees c for 1 h) has been developed, and results have been compared with those obtained with stand ...19846517320
stroke mortality and proportional expenditure on selected food items in japanese communities.proportional food expenditure was calculated from the 1959 national survey of family income and expenditures and 843 municipalities were trichotomized according to the relative expenditure of various food items. sex specific standardized mortality ratio (smr) of stroke (1969-83) was calculated for each trichotomized food expenditure subgroup and compared. results indicated that western style diet such as expenditure of meat, milk and egg and bread had negative association with smr. the expenditu ...19846535436
dieldrin, heptachlor, and chlordane residues in soybeans in illinois 1974, 1980.the illinois soybean crop was monitored in 1974 and 1980 for dieldrin, heptachlor, and chlordane residues resulting from the use of these compounds for corn soil insect control in the years before extensive soybean cultivation. residue levels were compared with past published amounts. dieldrin residue levels in soybeans declined between 1974 and 1980. heptachlor and heptachlor epoxide levels remained level between 1974 and 1980. chlorinated hydrocarbon residues were lower in the southern third o ...19846538889
uranium effects on the growth of soybean (glycine max (l.) merr.). 19846539630
molecular characterization of a deletion mutation affecting the alpha'-subunit of beta-conglycinin of soybean.a naturally occurring variety of soybean glycine max cv. keburi, which lacks the alpha'-subunits of the 7s seed storage protein (beta-conglycinin), was recently described by kitamura and kaizuma. keburi beta-conglycinins contain a normal complement of alpha-, beta-, and gamma-subunits that accumulate in a temporally and spatially regulated manner comparable to that of the standard cultivar provar. poly(a)+ rna isolated from mid-to-late maturation stage keburi seeds was translated in vitro, yield ...19846539803
[characterization of saponin constituents in soybeans of various origins and quantitative analysis of soyasaponins by gas-liquid chromatography]. 19846539824
phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mrnas and correlation with phytoalexin accumulation.a glucan elicitor from cell walls of the fungus phytophthora megasperma f. sp. glycinea, a pathogen of soybean (glycine max), induced large and rapid increases in the activities of enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase, and of the flavonoid pathway, acetyl-coa carboxylase and chalcone synthase, in suspension-cultured soybean cells. the changes in phenylalanine ammonia-lyase and chalcone synthase activities were correlated with corresponding changes in the mrn ...19846540068
molecular species of plant phosphatidylinositol with selective cytotoxicity towards tumor cells.the molecular species of highly purified phosphatidylinositol from soybeans were determined as an aid in the investigation of the mechanism of their reported selective cytotoxicity towards tumor cells. unlike the animal phosphatidylinositol, which contains predominantly stearic acid in the sn-1 and arachidonic in the sn-2 position (18:0 20:4), the soybean phosphatidylinositol was found to contain mainly linoleic acid in the sn-2 position and palmitic (16:0 18:2), stearic (18:0 18:2) and linoleic ...19846540602
[quantitative determination of soyasaponins in soybeans of various origins and soybean products by means of high performance liquid chromatography]. 19846540805
identification of benzo[a]pyrene quinone in soybean leaves.soybean seeds (glycine max) were grown in soil containing 10 ppm of benzo[a]pyrene (bap). after 76 days growth, three kinds of quinones, namely bap-1,6-, 3,6- and 6,12-quinones, were isolated and identified. however, the formative mechanism of the quinones in leaves has yet to be determined.19846541371
localization of four genes for three trnaleu's on the physical map of the soybean (glycine max l.) chloroplast genome.three trnasleu from soybean chloroplasts were isolated and hybridized to restriction fragments of soybean chloroplast dna. based on the hybridization pattern, the locations of four genes coding for trna1leu, trna2leu (two genes trna2aleu and trna2bleu, are present in the inverted repeat region) and trna3leu were determined on the physical map of the soybean chloroplast genome.19846541611
the amino acid sequence of the a2b1a subunit of glycinin.the amino acid sequences of the acidic and basic components of the a2b1a subunit of glycinin, the major seed reserve protein of the soybean (glycine max l. merr.), were determined. they contain 278 and 180 amino acids, respectively, and have molecular weights of 31,600 +/- 100 and 19,900 +/- 100. the molecular weight of the acidic component is considerably less than that estimated by sodium dodecyl sulfate-gel electrophoresis (37,000). sequence heterogeneity was detected at several positions sca ...19846541652
the complete amino acid sequence of the a3 subunit of the glycinin seed storage protein of the soybean (glycine max (l.) merrill).the amino acid sequence of the a3 subunit, an acidic subunit of the glycinin seed storage protein of the soybean (glycine max (l.) merrill), has been determined by sequence analysis of the peptides obtained by cyanogen bromide cleavage followed by enzymatic digestion and partial acid hydrolysis. the a3 subunit is composed of 410 amino acids (mr approximately 46,000). it has been confirmed that there are conserved, repeated domains within the subunit. a comparison of the nh2-terminal sequence of ...19846542104
phosphatidylinositol turnover in isolated soybean membranes stimulated by the synthetic growth hormone 2,4-dichlorophenoxyacetic acid.phospholipids of plant membranes isolated from homogenates of dark-grown hypocotyls of soybean (glycine max l.) undergo rapid and specific degradative changes. the degradation of phosphatidylinositol (pi) in such membranes is enhanced in the presence of the synthetic auxin, 2,4-dichlorophenoxyacetic acid (2,4-d), measured as the hydrolysis of pi or by an enhancement of [3h]inositol incorporation into membrane-associated pi stimulated by mn2+, but not dependent upon added ctp, mg2+, or diglycerid ...19846542567
studies on the inhibition of pancreatic and microbial lipases by soybean proteins.a protein, molecular weight 70,000 that inhibits pancreatic lipase has been isolated from soybean seeds. inhibition is not reversed by colipase unless bile salts are added to the assay system. inhibitory properties of the purified protein are very similar to those of serum albumin or alpha-lactoglobulin. it has been confirmed that, during intestinal lipolysis of dietary fats, bile salts play an essential role for the activation of the lipase-colipase system in the presence of inhibitory proteins ...19846542933
[genetic improvement of grain legumes of importance to latin american diet and the soy bean].common beans and soybeans can be considered as the most important food legumes in the latin american nutritional context, each of them presenting specific problems. the production of common beans has not increased in the last decade, and insects and diseases attacks are menacing it. on the other hand, soybeans have shown a tremendous increase in production, becoming rapidly an important export cash crop. however, its use in human consumption is quite restricted, in spite of the efforts of some c ...19846543422
the use of blends of cassava flour and extruded full-fat soybeans in diets for broiler chickens.a study was conducted to determine the effects of blending different levels of a low-prussic acid cassava flour with extruded full-fat soybeans in diets for growing broiler chickens. the full-fat soybeans contribute oil which increases the energy content of the diet, aids in overcoming the dusty nature of cassava, and provide high-quality protein. one-third, two-thirds, and all of the maize was replaced by cassava in diets with none, 12.5 and 25% extruded full-fat soybeans. diets were fed in pel ...19846544063
flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines.the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ...19846609053
a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk.eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ...19836615758
intracellular occurrence of cowpea mild mottle virus in two unrelated plant species.the filamentous particles of cowpea mild mottle virus (cmmv) were detected only in the cytoplasm of palisade, mesophyll, parenchyma and epidermal cells of glycine max (papilionaceae) and nicotiana clevelandii (solanaceae). they were aggregated to form either sheets or bundles or, more frequently, brush-like inclusions. although cmmv was initially considered to be a possible member of the carlavirus group, it differs from aphid-borne carlaviruses in its intracellular occurrence and transmission b ...19836629699
adenine binding sites of the lectin from lima beans (phaseolus lunatus).a single high-affinity binding site for adenine and related compounds was identified in the lima bean lectin (lbl) component iii tetramer. this site is identical with the high affinity site for 2,6-toludinyl-naphthalenesulfonate described previously (roberts, d. d., and goldstein, i. j. (1982) j. biol. chem. 257, 11274-11277). [14c]adenine was bound with high affinity (kd = 1.2 +/- 0.1 x 10(-5) m, t = 25 degrees c) and a high degree of specificity in that hypoxanthine and guanine were very poor ...19836643455
elements in major raw agricultural crops in the united states. 1. cadmium and lead in lettuce, peanuts, potatoes, soybeans, sweet corn, and wheat. 19836655141
elements in major raw agricultural crops in the united states. 2. other elements in lettuce, peanuts, potatoes, soybeans, sweet corn, and wheat. 20156655142
trypsin inhibitor effects on food intake and weight gain in zucker rats.decreased body weight and increased pancreas weight which occur in rats fed raw soybeans are thought to be due to the presence of trypsin inhibitors in the soybeans (sbti). since trypsin is postulated to be a negative feedback signal for cholecystokinin (cck) secretion, sbti may have these effects by increasing secretion of cck. cck is a putative satiety signal; thus, increased secretion of cck could decrease food intake, and, if maintained over a period of time, body weight. in these experiment ...19836657770
chemical composition of winged bean (psophocarpus tetragonolobus) varieties.the chemical composition of dry seeds of four varieties, pods, stalks and leaves of winged beans (psophocarpus tetragonolobus) was determined. the seeds had a high range of protein (27.8-36.6%) and fat (14.8-17.9%), which were similar to soybeans. the seeds contained high phosphorus, calcium and magnesium. the leaf was highest in protein content (33.7%) of all the parts studied except for the seeds. the protein and fat content of pods decreased as pods ripened. the calcium content in the leaf wa ...19836663368
bowman-birk soybean protease inhibitor as an anticarcinogen.vegetarian populations show a decreased occurrence of breast, colon, and prostatic cancers. epidemiological studies have identified seeds (maize, corn, and beans) as protective agents in these cancers. we have selected to study one abundant component of all seeds, protease inhibitors. synthetic and natural protease inhibitors have been shown to inhibit tumor promotion in vivo and in vitro. in the present study, we report that a typical, natural protease inhibitor, the bowman-birk inhibitor isola ...19836682011
labeling of soybeans with the stable isotope 70zn for use in human metabolic studies.radiozinc (65zn) was used to assess the efficiency of incorporation of the label into soybean seeds by means of a noncycling hydroponic system. it was found that 21.3 and 27.6% of the applied dose was incorporated into the mature seeds when the tracer was applied during vegetative or flowering stages of growth cycle, respectively. the latter method was adapted to labeling with the stable isotope 70zn, and quantitative incorporation of this isotope was investigated within and among hydroponic cul ...19836682436
determination of cyanide in soybeans and soybean products. 20006682869
varietal influence on the quantity of glycinin in soybeans. 20066682870
characterization of a soybean cultivar lacking certain glycinin subunits.the 11s storage protein (glycinin) of soybean [glycine max (l.) merr., cv. raiden] was studied by polyacrylamide gel electrophoresis and amino acid sequence analysis. it contained the following subunits composed of acidic (a) and basic (b) polypeptides: a1ab2, a1bb1b, a2b1a, and a3b4. however, it lacked polypeptides a4, a5, and b3 which are present in many other cultivars. a new acidic polypeptide called a6 was present in a low amount and was characterized by amino acid sequence analysis. it was ...19836683095
lectin receptors on the plasma membrane of soybean cells. binding and lateral diffusion of lectins.protoplasts prepared from suspension cultures of root cells of glycine max (sb-1 cell line) bound soybean agglutinin (sba), concanavalin a (con a), and wheat germ agglutinin (wga). binding studies carried out with 125i-labeled sba, con a, and wga showed that these interactions were saturable and specific. fluorescence microscopy demonstrated uniform membrane labeling. the mobility of the lectin-receptor complexes was measured by fluorescence redistribution after photobleaching. the diffusion con ...19836684479
stereospecific analysis of triglycerides of glycine max, glycine soya, avena sativa and avena sterilis strains.a rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number of glycine max, glycine soya, avena sativa and avena sterilis strains. the percentage of each fatty acid on the sn-1-, sn-2- and sn-3-positions was linearly related to the total percentage of the fatty acid in the triglyceride. large deviations from the common triglyceride pattern were not found.19836686643
exogenous methionine depresses level of mrna for a soybean storage protein.in vitro translation experiments indicate that absence of the beta-subunit of 7s storage protein in soybean (glycine max l. merr. cv. provar) cotyledons cultured on methionine-supplemented medium is due to lack of functional mrna for that polypeptide. relative amounts of functional mrna for the 7s alpha'- and alpha-subunits were unaffected by methionine in the cotyledon culture medium. measurements of beta-subunit accumulation in cotyledons transferred from basal medium to methionine-supplemente ...19836686764
[whole soy bean flour: use of a methodology of response surface for the study of nutritional aspects].cotyledons of soybeans (glycine max) of the paraná variety were subjected to hydrothermal processing. response surface methodology was used to evaluate the conditions which provided a product of the highest protein quality. the processing variables used in the experimental design were: soaking time (0 - 8 hr); blanching time (5 - 35 min) and bicarbonate concentration (0 - 0.5 g%) in blanching water. biological evaluations of protein quality were done using the npr. the mathematical model develop ...19836687106
iron absorption from legumes in humans.iron bioavailability in a variety of legumes was determined by radioisotopic measurements of iron absorption in human subjects. soybeans, black beans, lentils, mung beans, and split peas were prepared as soups, labeled by the extrinsic tag method, and served to fasting subjects. mean percentage absorption was uniformly low, ranging from 0.84 to 1.91%. differences in absorption between the means were not statistically significant. the results indicate that these five commonly eaten legumes are al ...19846741854
legume lipids.the storage lipids of legume seeds are a major source of dietary fat. as a result of their importance in the food industry, much is known about lipid composition, chemistry, flavor, off-flavor development, and their technological implications in foods of dry, oil-rich seeds such as soybeans and peanuts. lipids from green pea have also been investigated to some extent. other food legume lipids have not been studied in any great detail because of their low lipid content and limited or negligible u ...19826751699
a new mitogenic d-galactosephilic lectin isolated from seeds of the coral-tree erythrina corallodendron. comparison with glycine max (soybean) and pseudomonas aeruginosa lectins.the lectin of erythrina corallodendron (caesalpiniaceae) seeds was purified by heating, ammonium sulfate fractionation, and affinity chromatography on acid-treated sepharose. the purified lectin is similar to the soybean lectin in being a glycoprotein of molecular weight around 110 000 - 120 000 and having d-galactosephilic activity. this lectin, like the soybean and pseudomonas aeruginosa lectins, binds to d-galactosamine, n-acetyl-d-galactosamine, alpha- and beta-galactosides as well as to d-g ...19816790142
the effects of bivalent cations on ribulose bisphosphate carboxylase/oxygenase.the half-saturation constants for binding of the bivalent cations (mg2+, ni2+, co2+, fe2+ and mn2+) to ribulose bisphosphate carboxylase/oxygenase from glycine max and rhodospirillum rubrum were measured. the values obtained were dependent on the enzyme and the cation present, but were the same for both oxygenase and carboxylase activities. ribulose bisphosphate rather than its cation complex was the true substrate. the kinetic parameters vmax.(co2), vmax.(o2), km(co2), km(o2), and k1(o2) were d ...19816796054
a study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid.lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of 16o2 and 18o2. the formation of 16o/18o-molecules which is indicative for a head-to head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.19816798993
specificities of antisera directed against soybean lipoxygenases-1 and -2 and purification of lipoxygenase-2 by affinity chromatography.antiserum directed against lipoxygenase-1 from soybean (glycine max (l). merr. var. williams) was developed in rabbits with electrophoretically pure lipoxygenase-1 as an antigen. to remove traces of lipoxygenase-1 from a lipoxygenase-2 preparation the latter was chromatographed over a column of sepharose 4b to which antibodies directed against lipoxygenase-1 were coupled. during affinity chromatography of lipoxygenase-2 no protein and only a small amount of activity were lost. affinity-purified ...19826816287
lectins as markers of human epidermal cell differentiation.the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ...19826816653
protein quality and oil digestibility of lupinus mutabilis: metabolic studies in children.the nutritional quality of lupins (lupinus mutabilis) for infants and children was evaluated in two sets of balance studies. in the first the digestibility and protein quality of diets based on lupin flour, with and without methionine supplementation, were compared with those of a control diet consisting of casein, sucrose and vegetable oil. apparent nitrogen absorption from lupin flour (81.8 and 84.3% of intake) was slightly but significantly less than that during casein control periods (87.2 a ...19836834148
a soybean lectin having 4-o-methyl-d-glucuronic acid specificity.evidence is presented for the presence of a new lectin activity in soybean seeds [glycine max (l.) merrill] that has specificity towards the 4-o-methyl-d-glucurono-l-rhamnan exopolysaccharide produced by certain strains of rhizobium japonicum. bacterial agglutination and precipitin reactions revealed the lectin activity in phosphate-buffered saline extracts of seeds of all cultivars tested, including the "lectinless" varieties. reaction of such extracts with carbohydrate haptens demonstrated tha ...19836838587
mutagenicity, carcinogenicity and reproductive effects of dibromochloropropane (dbcp).dibromochloropropane (dbcp), a brominated organochlorine nematocide, has been used since the mid-1950s. its primary value was its effectiveness on perennial crops without damaging the plants. dbcp also had less acute toxicity than earlier soil fumigants such as ethylene dibromide. dbcp became widely used on citrus, grapes, peaches, pineapple, soybeans and tomatoes. in central america and israel, banana trees were treated with dbcp. by 1975, 25 million pounds were being produced in the u.s. per y ...19836888412
phenolic acids in oilseed flours.noticeable quantitative differences were found in the content of free and bound phenolic acids in flours obtained from soybeans, cottonseed, peanuts, rapeseed, white mustard, flax and sesame seeds. independently of the type of seeds used to produce flour, the highest amounts of acids were liberated from ester bonds. these acids were especially abundant in flour made from rape and white mustard seeds. phenolic compounds identified in these flours were predominated by sinapic and p-hydroxybenzoic ...19836888524
soybeans vs. textured soy proteins as meat extenders. cooking losses, palatability, and thiamin content of freshly cooked and frozen meat loaves.the effects of replacing 30 percent of the ground beef in meat loaves with boiled ground soybeans or textured soy protein (tsp) were studied. the substitutions were equally effective in increasing yields of freshly cooked, raw frozen and cooked, and cooked, frozen, and reheated meat loaves. scores for several palatability characteristics, including general desirability, were higher for meat loaves containing soybeans than for loaves containing tsp. the mixtures and loaves contained similar amoun ...19826889616
extraction procedures for oilseeds and related high fat-low moisture products.a combined sample preparation/extraction procedure is presented for pesticide residue analysis of oilseeds and related high fat-low moisture products. the procedure utilizes high-speed milling to prepare the sample and high-speed homogenization in the extraction step to achieve what is apparently quantitative isolation of both incurred residues and natural oils. a separate, simple, oil determination step allows findings to be reported on either the fat or whole product basis. petroleum ether, et ...19826890060
soybean (glycine max [l.] merrill) as a short-term assay for study of environmental mutagens. a report of the u.s. environmental protection agency gene-tox program.the soybean (glycine max [l.] merrill) spot test is suggested as a preliminary screening test for environmental mutagens. this system makes use of various types of spots that originate from the treatment of seeds or seedlings with mutagens. the homozygous dominant y11y11 dark green leaves may show light green and very dark green spots; the heterozygous y11y11 light green leaves may show dark green, yellow or twin spots; and the homozygous recessive y11y11 yellow leaves show light green receptors ...19826891026
zinc absorption from composite meals. ii. influence of the main protein source.the absorption of zinc in man from composite meals of conventional foods with various main protein sources was measured with a radionuclide technique. the meals were extrinsically labeled with 65zn and the absorption of zinc was determined from a measurement of the whole-body retention of the isotope about 2 weeks after ingestion of the labeled meal. the meals consisted of a meat patty made from either chicken, beef, or soybeans, and included potatoes, tomatoes, and white bread. for meals based ...19806893252
structural sequences are conserved in the genes coding for the alpha, alpha' and beta-subunits of the soybean 7s seed storage protein.cloned dnas encoding four different proteins have been isolated from recombinant cdna libraries constructed with glycine max seed mrnas. two cloned dnas code for the alpha and alpha'-subunits of the 7s seed storage protein (conglycinin). the other cloned cdnas code for proteins which are synthesized in vitro as 68,000 d., 60,000 d. or 53,000 d. polypeptides. hybrid selection experiments indicate that, under low stringency hybridization conditions, all four cdnas hybridize with mrnas for the alph ...19826897678
effects of 42- and 2450-mhz dielectric heating on nutrition-related properties of soybeans.dielectric heating at frequencies of 42 and 2450 mhz was applied to whole soybeans of natural moisture content for varies exposure times. the minimum energy absorbed (mea) was calculated from moisture-loss and temperature-elevation data. biochemical analyses were performed to determine protein dispersibility index (pdi), nitrogen solubility index (nsi), and trypsin-inhibitor, urease, lipoxygenase, and peroxidase activities. because the heating rates were different at the two frequencies for the ...19816920415
lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins.in cellular immunology, peanut (arachis hypogaea) lectin has been used to selectively agglutinate immature lymphoid cells and soybean (glycine max-lectin to agglutinate b lymphocytes. we have used affinity chromatography to study the surface glycoproteins of rat and mouse lymphoid cells which bind to these lectins. thymocyte and t and b lymphocyte glycoproteins were analysed either without modification (native) or after the removal of sialic acid with neuraminidase (asialo). the only native glyc ...19826980823
the role of lectins in blood group serology.many lectins display blood group activity, and extracts from dolichos biflorus (anti-a1), ulex europaeus (anti-h), and vicia graminea (anti-n) seeds provide an alternative to human sera as a source of blood-typing reagents. however, the major application of lectins in blood group serology undoubtedly lies in the recognition and elucidation of red celll polyagglutination. in this respect, lectins from arachis hypogaea (anti-t/tk), salvia sclarea (anti-tn). salvia horminum (anti-tn + cad). dolicho ...19806998652
mutagenicity of municipal water obtained from an agricultural area.we have studied the mutagenicity of the water from lake bloomington and of the tap water that is made from the lake water. the lake, which is the source of drinking water for bloomington, illinois (pop. 44,000), is surrounded by land that is farmed intensively--being mainly in maize and soybeans. samples were collected monthly from may through october 1979 and concentrated 3,000x with xad-2 resin. nearly all of the lake and tap water concentrates were mutagenic in salmonella typhimurium ta98 and ...19817026225
clotting and other plasma factors in experimental endotoxemia: inhibition of degradation by exogenous proteinase inhibitors.endotoxemia in dogs was induced by a slow intravenous infusion of e. coli endotoxin for 2 h. thereby, a significant decrease was observed in the plasma levels of several clotting, fibrinolysis and complement factors. the changes were studied over an experimental period of 14 h and checked for statistical significance by three-way analysis of variance. application of the broad-spectrum proteinase inhibitor aprotinin (trasylol) from bovine organs clearly lowered the endotoxin-induced decline of th ...19817028486
the effects of soybean trypsin inhibitors on the pancreas of animals and man: a review.human trypsin is more resistant to inhibition than is the trypsin of other mammalian species. the effect on human trypsin of soybean trypsin inhibition in soy protein does not appear to be a potential hazard to man. therefore, the elimination of sti does not seem to be necessary for humans. in animal diets, however, pancreatic toxicity must be considered whenever soybean protein is utilized. soybeans should be treated to increase their nutritional benefits and decrease any animal health risks (2 ...19827036513
biosynthetic control of the natural abundance of carbon 13 at specific positions within fatty acids in saccharomyces cerevisiae. isotopic fractionation in lipid synthesis as evidence for peroxisomal regulation.measurements of the natural abundance of 13c at c-1, c-9, and c-10 in fatty acids synthesized by saccharomyces cerevisiae grown aerobically at 30 degrees c show that alkyl chain positions derived from the carboxyl group of the acetate precursor must be enriched in 13c by 2.5 +/- 0.6 parts per thousand while those derived from the methyl group in acetate must be depleted in 13c by an equal amount. selective depletions of 13c observed at the c-9 and c-10 positions of palmitoleate and oleate requir ...19827040368
enzymic synthesis of lignin precursors. comparison of cinnamoyl-coa reductase and cinnamyl alcohol:nadp+ dehydrogenase from spruce (picea abies l.) and soybean (glycine max l.).cambial sap of spruce (picea abies) proved to be a good source for isolation of cinnamoyl-coa reductase and cinnamyl alcohol:nadp+ dehydrogenase. apparently homogeneous enzymes were obtained by a multistep procedure including dye-ligand chromatography and for the reductase also affinity chromatography on (coenzyme a)-agarose. an improved purification procedure for the reductase from soybean cell cultures is also reported. molecular weights and subunit composition of reductase and dehydrogenase f ...19817042334
use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa.paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ...19827130674
[effect of protease inhibitors on influenza virus reproduction].natural inhibitors of proteases have been shown to inhibit plaque production by influenza virus. investigation of the fate of viral intracellular proteins revealed the inhibiting effect of these inhibitors on their posttranslation cutting. it is assumed, therefore, that the antiviral effect of the preparation under study is associated with inhibition of viral proteins cutting, in particular, of hemagglutinin. a comparative study of the antiviral activity of inhibitors of proteases from various s ...19827135926
effect of esterification and side-chain alkylation on alteration of translocation characteristics of methamidophos.1. the phloem mobility and systemic activity of methamidophos (i); propanedioic acid 1-phosphoramidothionic acid o,s-dimethyl ester, 2-methyl, 3-0monoethyl ester (ii); propanedioic acid, 1-phosphoramidothionic acid o,s-dimethyl ester, 2,2-dimethyl, 3-monoethyl ester (iii); and butenoic acid, 1-monoethyl ester, 2,3,-e & z, 3-phosphoramidothionic acid o,s-dimethyl ester (iv) were investigated in soybeans (glycine max l.) with a bioassay using cabbage looper (tricoplusia ni hüber) larvae to determi ...19827147994
circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols.the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ...19827150429
lipid molecular species composition of thylakoid membranes.lipid molecular species compositions of chloroplast thylakoid membranes of mesophyll cells from spinacia oleracea, glycine max, oryza sativa and zea mays and of bundle sheath cells from zea mays have been quantitatively determined. no significant difference in the lipid molecular species composition was found among the five membrane sources. the predominant molecular species of monogalactosyldiacylglycerol was the 1-linolenoyl parallel to 2-linolenoyl species. the 1-linolenoyl parallel to 2-lino ...19807188732
ultrastructural localization of soybean agglutinin on thin sections of glycine max (soybean) var. altona by the gold method.soybean agglutinin (sba) has been localized in glycine max (soybean) var. altona at the ultrastructural level by the gold method. sba was detected by marking thin sections of different part of the seed with gold granules (12 nm in size) labelled with anti-sba antiserum. upon examination by transmission electron microscopy, the lectin was found uniformly distributed in most of the protein bodies of the cotyledon. sba was also present in the embryo axis.19807188935
lectins and the soybean-rhizobium symbiosis. i. immunological investigations of soybean lines, the seeds of which have been reported to lack the 120 000 dalton soybean lectin.seeds of six soybean lines (glycine max (l.) merr. cv. columbia, d68-127, norredo, sooty, t-102, wilson 5) have been reported to lack the 120 000 dalton soybean lectin. immunodiffusion and radioimmunoassay using anti-soybean lectin immunoglobulin failed to detect the lectin in seeds of five lines, but d68-127 seeds contained as much soybean lectin as the control line, harosoy 63. the d68-127 seed lectin could be purified by affinity chromatography on sepharose-n-caproylgalactosamine, and was ind ...19807190028
nodulation of soybeans carrying the nodulation-restrictive gene, rj1, by an incompatible rhizobium japonicum strain upon mixed inoculation with a compatible strain.the rj1 gene in soybeans prevents nodulation by most strains of rhizobium japonicum. several strains, however, are known to nodulate rj1 plants in vermiculite or sand culture. pure broth cultures of one of these strains (61 nalr) and a strain producing the typical non-nodulating response with rj1 (i-110 ars) were mixed and used as inoculum on clark rj1 soybeans in a growth chamber experiment. both strains carried drug resistance markers and were identified using selective media. analysis of the ...19807190864
effect of raw legume diets on disaccharidase activity in the small intestine of chicks.the effect of four raw legume diets: field beans (vicia faba) (rfb), navy beans (phaseolus vulgaris) (rnb), soybeans (glycine soja) (rsb) and bitter vetch (vicia ervilia) (rbv), on disaccharidase activities in chick small intestine have been studied. maltase and sucrase activities, which vary with age, were determined in 1 to 60 day old animals, rfb and rbv diets had no effect on maltase activity and only increased sucrase activity in 60 day old chicks. both maltase and sucrase activities decrea ...19807192009
circular dichroism tests on the effect of alkali on conformation of lectins.the structural properties of eight lectins (agglutinins) were studied by the circular dichroism (cd) probe. the lectin from maclura pomifera (osage orange seed) exhibited in the far-ultraviolet spectral zone a positive cd band centered at 201 nm, whereas the other lectins had positive bands at 195--198 nm. structural transitions were effected by alkali, ph 9.6--11.9. native m. pomifera lectin had a positive cd band also at 236 nm which shifted to the red and enhanced by alkali. significant indiv ...19807192161
effects of ingesting soy or egg lecithins on serum choline, brain choline and brain acetylcholine.rats were fed lecithins, derived from eggs or soybeans, to determine whether the fatty acid composition of the phosphatidylcholine altered choline availability. rats were fed either a single meal containing 5 g phosphatidylcholine or a lecithin-containing diet for 3 weeks, including approximately 5 g phosphatidylcholine per day. each form of dietary lecithin elevated blood choline, brain choline and brain acetylcholine significantly (p < 0.05). there was no difference in response to egg- or soy- ...19817192727
partial characterization of the polyisoprenoid carrier of n-acetylglucosamine in glycine max (soya bean).dolichyl phosphate (c55) and dolichyl phosphate prepared from liver were incubated with an enzyme prepared from soya-bean protoplasts. they both stimulated the transfer of radioactivity from udp-d-glucose to lipid, but the stimulation was greater with liver dolichyl phosphate. liver dolichyl phosphate with the soya-bean enzyme stimulated the transfer of radioactivity from udp-n-acetyl-d-[u-14c]glucosamine to acidic lipid. udp-d-[u-14c]glucose and udp-n-acetyl-d-[u-14c]glucosamine were used with ...19807193454
interaction of lectins from soybean and peanut with rhizobia that nodulate soybean, peanut, or both plants.four of 14 strains of rhizobium japonicum from soybean nodulated peanut (arachis hypogaea l. cultivar jumbo virginia), and 3 of 8 rhizobium sp. strains from peanut nodulate soybean (glycine max (l.) merr. cultivar harosoy 63). cells of three peanut rhizobia bound fluorescent- and radioisotope-labeled soybean lectin. two of these strains failed to nodulate soybean, and conversely, two peanut strains that nodulated soybean did not bind to soybean lectin. both culture medium and age had pronounced ...19807195297
[electron microscopic studies of globulin gels of soybeans]. 20067196264
soybean diet lowers breast tumor incidence in irradiated rats.this paper examines the relationship between feeding a diet rich in protease inhibitors and the reduction of mammary cancer induced by x-irradiation in sprague-dawley rats. of a total of 145 irradiated animals, 44% of the 45 rats fed a raw soybean diet containing a high concentration of protease inhibitor developed mammary tumors as compared to 74% of 50 rats fed a casein diet containing no protease inhibitor. animals fed purina rat chow which contained low levels of protease inhibitor exhibited ...19807196809
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