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effects of the veterinary pharmaceutical salinomycin and its formulation on the plant brassica rapa.veterinary pharmaceuticals are emerging contaminants found throughout the environment, and their presence and effects are a matter of concern. the purpose of this study was to compare the phytotoxicity of salinomycin (pure compound = 96 %) and sacox 120 (formulated product = 120 g salinomycin/kg) to the plant species brassica rapa as well as to investigate salinomycin persistence in soil. calculated ec/ic(50) values for salinomycin and sacox 120 were 1.10 and 2.88 and 2.19 and 18.03 mg/kg, respe ...201222961218
syntenic gene analysis between brassica rapa and other brassicaceae species.chromosomal synteny analysis is important in genome comparison to reveal genomic evolution of related species. shared synteny describes genomic fragments from different species that originated from an identical ancestor. syntenic genes are orthologs located in these syntenic fragments, so they often share similar functions. syntenic gene analysis is very important in brassicaceae species to share gene annotations and investigate genome evolution. here we designed and developed a direct and effic ...201222969786
development of indel markers for brassica rapa based on whole-genome re-sequencing.genome-wide detection of short insertion/deletion length polymorphisms (indels, <5 bp) in brassica rapa (named the a genome) was performed by comparing whole-genome re-sequencing data from two b. rapa accessions, l144 and z16, to the reference genome sequence of chiifu-401-42. in total, we identified 108,558 indel polymorphisms between chiifu-401-42 and l144, 26,795 indels between z16 and chiifu-401-42, and 26,693 indels between l144 and z16. from these, 639 indel polymorphisms of 3-5 bp in leng ...201322972202
identification and characterization of orthologs of atnhx5 and atnhx6 in brassica napus.improving crop species by breeding for salt tolerance or introducing salt tolerant traits is one method of increasing crop yields in saline affected areas. extensive studies of the model plant species arabidopsis thaliana has led to the availability of substantial information regarding the function and importance of many genes involved in salt tolerance. however, the identification and characterization of a. thaliana orthologs in species such as brassica napus (oilseed rape) can prove difficult ...201222973287
determining the geographical origin of chinese cabbages using multielement composition and strontium isotope ratio analyses.recently, the korean market has seen many cases of chinese cabbage (brassica rapa ssp. pekinensis) that have been imported from china, yet are sold as a korean product to illegally benefit from the price difference between the two products. this study aims to establish a method of distinguishing the geographical origin of chinese cabbage. one hundred chinese cabbage heads from korea and 60 cabbage heads from china were subjected to multielement composition and strontium isotope ratio ((87)sr/(86 ...201222980856
a large insertion in bhlh transcription factor brtt8 resulting in yellow seed coat in brassica rapa.yellow seed is a desirable quality trait of the brassica oilseed species. previously, several seed coat color genes have been mapped in the brassica species, but the molecular mechanism is still unknown. in the present investigation, map-based cloning method was used to identify a seed coat color gene, located on a9 in b. rapa. blast analysis with the arabidopsis genome showed that there were 22 arabidopsis genes in this region including at4g09820 to at4g10620. functional complementation test ex ...201222984469
mapping and candidate-gene screening of the novel turnip mosaic virus resistance gene retr02 in chinese cabbage (brassica rapa l.).the extreme resistance to turnip mosaic virus observed in the chinese cabbage (brassica rapa) line, bp8407, is monogenic and recessive. bulked segregant analysis was carried out to identify simple sequence repeat and indel markers linked to this recessive resistance gene, termed recessive turnip mosaic virus resistance 02 (retr02). mapping of pcr-specific indel markers on 239 individuals of a bp8407 × ji zao chun f(2) population, located this resistance gene to a 0.9-cm interval between two inde ...201322996569
glucosinolate variation in leaves of brassica rapa crops.total and individual glucosinolate (gsl) content of leaves of vegetable turnip rape (brassica rapa l. var. rapa) was determined in a set of 45 varieties consisting in early, medium and late types grown at two locations in northwestern spain. the objectives were to determine the diversity among varieties in gsl content and to relate that variation with earliness and plant habit. eight gsl were identified, being two aliphatic gsl, gluconapin (84.4 % of the total gsl) and glucobrassicanapin (7.2 % ...201223001436
enzymatic degradation of congo red by turnip (brassica rapa) peroxidase.the enzyme peroxidase is known for its capacity to remove phenolic compounds and aromatic amines from aqueous solutions and also to decolourize textile effluents. this study aims at evaluating the potential of a turnip (brassica rapa) peroxidase (tp) preparation in the discolouration of textile azo dyes and effluents. an azo dye, congo red (cr), was used as a model pollutant for treatment by the enzyme. the effects of various operating conditions like ph value, temperature, initial dye and hydro ...201323016283
genome-wide identification, classification and expression analysis of genes encoding putative fasciclin-like arabinogalactan proteins in chinese cabbage (brassica rapa l.).fasciclin-like arabinogalactan proteins (flas), a subclass of arabinogalactan proteins (agps), have both predicted agp-like glycosylated regions and putative fasciclin (fas) domains, which may function in cell adhesion and communication. previous studies have identified 21, 27, and 34 flas in arabidopsis (arabidopsis thaliana), rice (oryza sativa), and wheat (triticum aestivum), respectively. in this study, we identified 33 flas in the annotated genome of chinese cabbage (brassica rapa ssp. peki ...201223053954
molecular characterization of the cra gene conferring clubroot resistance in brassica rapa.clubroot disease is one of the major diseases affecting brassicaceae crops, and a number of these crops grown commercially, such as chinese cabbage (brassica rapa l. ssp. pekinensis), are known to be highly susceptible to clubroot disease. to provide protection from this disease, plant breeders have introduced genes for resistance to clubroot from the european turnip into susceptible lines. the cra gene confers specific resistance to the clubroot pathogen plasmodiophora brassicae isolate m85. fi ...201223054353
molecular characterization of the brassica rapa auxin-repressed, superfamily genes, brarp1 and brdrm1.two auxin-repressed superfamily genes, auxin-repressed protein 1 (arp1) and dormancy-associated protein 1 (drm1), are highly expressed in both the dormant buds and non-growing tissues of several plant species. to further identify the function of these proteins in chinese cabbage (brassica rapa l. ssp. pekinensis), we examined comprehensive expression patterns of brarp1 and brdrm1 under various developmental and stress conditions. we also examined these same genes in transgenic arabidopsis plants ...201323065269
a hypomethylated population of brassica rapa for forward and reverse epi-genetics.epigenetic marks superimposed on the dna sequence of eukaryote chromosomes provide agility and plasticity in terms of modulating gene expression, ontology, and response to the environment. modulating the methylation status of cytosine can generate epialleles, which have been detected and characterised at specific loci in several plant systems, and have the potential to generate novel and relatively stable phenotypes. there have been no systematic attempts to explore and utilise epiallelic variat ...201223082790
metabolism of soluble rapeseed meal (brassica rapa l.) protein during incubations with buffered bovine rumen contents in vitro.accurate quantitative information on the fate of dietary protein in the rumen is central to modern metabolizable protein systems developed to improve the efficiency of nitrogen utilization in ruminants. an in vitro method was developed to estimate the rate of soluble rapeseed meal (brassica rapa l.) protein (srmp) degradation. unlabeled and (15)n-labeled solvent-extracted rapeseed meal were incubated alone or as an equal mixture (125 mg of n/l) with buffered rumen contents and a mixture of carbo ...201323127902
phenophyte: a flexible affordable method to quantify 2d phenotypes from imagery. 201223131141
production and characterization of an alloplasmic and monosomic addition line of brassica rapa carrying the cytoplasm and one chromosome of moricandia arvensis.intergeneric hybridization was performed between moricandia arvensis and four inbred lines of brassica rapa following embryo rescue. three f(1) hybrid plants were developed from three cross combinations of m. arvensis × b. rapa, and amphidiploids were synthesized by colchicine treatment. six bc(1) plants were generated from a single cross combination of amphidipolid × b. rapa 'ko1-303' through embryo rescue. one bc(2) and three bc(3) plants were obtained from successive backcrossing with b. rapa ...201123136474
microstructure of a brassica rapa genome segment homoeologous to the resistance gene cluster on arabidopsis chromosome 4.genome evolution is a continuous process and genomic rearrangement occurs both within and between species. with the sequencing of the arabidopsis thaliana genome, comparative genetics and genomics offer new insights into plant biology. the genus brassica offers excellent opportunities with which to compare genomic synteny so as to reveal genome evolution. during a previous genetic analysis of clubroot resistance in brassica rapa, we identified a genetic region that is highly collinear with arabi ...201223136528
positive- and negative-acting regulatory elements contribute to the tissue-specific expression of inner no outer, a yabby-type transcription factor gene in arabidopsis.the inner no outer (ino) gene, which encodes a yabby-type transcription factor, specifies and promotes the growth of the outer integument of the ovule in arabidopsis. ino expression is limited to the abaxial cell layer of the developing outer integument of the ovule and is regulated by multiple regions of the ino promoter, including pos9, a positive element that when present in quadruplicate can produce low-level expression in the normal ino pattern.201223148487
genome-wide characterization of the cbf/dreb1 gene family in brassica rapa.the c-repeat/dehydration-responsive element binding transcription factors (cbf/drebs) are important proteins in involved in responses to abiotic stress in plants. we identified ten brdreb1 genes belonging to the cbf/dreb1 gene family in the brassica rapa whole genome sequence, whereas six genes are found in the arabidopsis thaliana genome. the deduced amino acid sequences of the b. rapa genes showed conserved motifs shared with other known plant cbf/dreb1s. comparative analysis revealed that nin ...201223148914
evaluation of the predation capacity of podisus maculiventris (hemiptera: pentatomidae) on microtheca ochroloma (coleoptera: chrysomelidae) in field cages.the predation capacity of the spined soldier bug, podisus maculiventris (say) (hemiptera: pentatomidae), was evaluated at three release rates in field cages containing larvae of the yellowmargined leaf beetle, microtheca ochroloma stål (coleoptera chrysomelidae), a pest of organic crucifer (brassicaceae) crops in the united states. the experiment was conducted twice, in february-march 2009 and in february-march 2010. on the same day as the introduction of 132 first instars of m. ochroloma into t ...201223156169
sequencing of chloroplast genome using whole cellular dna and solexa sequencing technology.sequencing of the chloroplast (cp) genome using traditional sequencing methods has been difficult because of its size (>120 kb) and the complicated procedures required to prepare templates. to explore the feasibility of sequencing the cp genome using dna extracted from whole cells and solexa sequencing technology, we sequenced whole cellular dna isolated from leaves of three brassicarapa accessions with one lane per accession. in total, 246, 362, and 361 mb sequence data were generated for the t ...201223162558
identification and profiling of novel micrornas in the brassica rapa genome based on small rna deep sequencing.micrornas (mirnas) are one of the functional non-coding small rnas involved in the epigenetic control of the plant genome. although plants contain both evolutionary conserved mirnas and species-specific mirnas within their genomes, computational methods often only identify evolutionary conserved mirnas. the recent sequencing of the brassica rapa genome enables us to identify mirnas and their putative target genes. in this study, we sought to provide a more comprehensive prediction of b. rapa mir ...201223163954
discrimination of cabbage (brassica rapa ssp. pekinensis) cultivars grown in different geographical areas using ¹h nmr-based metabolomics.cabbage (brassica rapa ssp. pekinensis) is one of the most popular foods in asia and is widely cultivated in many countries for the production of lightly fermented vegetables. in this study, metabolomic analysis was performed to distinguish two cultivars of cabbage grown in different geographical areas, korea and china, using ¹h nmr spectroscopy coupled with multivariate statistical analysis. principal component analysis (pca) showed clear discrimination between extracts of cabbage grown in kore ...201323199992
genetic analysis of hybrid seed formation ability of brassica rapa in intergeneric crossings with raphanus sativus.a hybridization barrier leads to the inability of seed formation after intergeneric crossings between brassica rapa and raphanus sativus. most b. rapa lines cannot set intergeneric hybrid seeds because of embryo breakdown, but a b. rapa line obtained from turnip cultivar 'shogoin-kabu' is able to produce a large number of hybrid seeds as a maternal parent by crossings with r. sativus. in 'shogoin-kabu' crossed with r. sativus, developments of embryos and endosperms were slower than those in intr ...201323203221
the brassica napus blackleg resistance gene lepr3 encodes a receptor-like protein triggered by the leptosphaeria maculans effector avrlm1.lepr3, found in the brassica napus cv 'surpass 400', provides race-specific resistance to the fungal pathogen leptosphaeria maculans, which was overcome after great devastation in australia in 2004. we investigated the lepr3 locus to identify the genetic basis of this resistance interaction. we employed a map-based cloning strategy, exploiting collinearity with the arabidopsis thaliana and brassica rapa genomes to enrich the map and locate a candidate gene. we also investigated the interaction o ...201323206118
the putative phytocyanin genes in chinese cabbage (brassica rapa l.): genome-wide identification, classification and expression analysis.phytocyanins (pcs) are a plant-specific family of small copper-containing electron transfer proteins. pcs may bind with a single copper atom to function as electron transporters in various biological systems, such as copper trafficking and plant photosynthesis. evidence indicates that pcs may also be involved in plant developmental processes and stress responses. many pcs possess arabinogalactan protein-like regions and are therefore termed chimeric arabinogalactan proteins (cagps). previously, ...201323212439
correlations between tocopherol and fatty acid components in germplasm collections of brassica oilseeds.to date, little is known about the correlations among the tocopherol (t) and fatty acid (fa) components in rapeseed oils. in the current study, a germplasm collection of landraces from the species brassica juncea , brassica rapa , and brassica napus and a collection of low erucic acid (ea) breeding lines from b. napus were analyzed for fa and t contents. in the groups comprising landraces, the most notable correlation was the significantly positive one between α-t and the sum of c18:1 and c18:2, ...201323214441
quantitative trait loci mapping in brassica rapa revealed the structural and functional conservation of genetic loci governing morphological and yield component traits in the a, b, and c subgenomes of brassica species.brassica rapa is an important crop species that produces vegetables, oilseed, and fodder. although many studies reported quantitative trait loci (qtl) mapping, the genes governing most of its economically important traits are still unknown. in this study, we report qtl mapping for morphological and yield component traits in b. rapa and comparative map alignment between b. rapa, b. napus, b. juncea, and arabidopsis thaliana to identify candidate genes and conserved qtl blocks between them. a tota ...201323223793
identificaiton of a clubroot resistance locus conferring resistance to a plasmodiophora brassicae classified into pathotype group 3 in chinese cabbage (brassica rapa l.).in chinese cabbage (brassica rapa), the clubroot resistance (cr) genes crr1 and crr2 are effective against the mild plasmodiophora brassicae isolate ano-01 and the more virulent isolate wakayama-01, but not against isolate no. 14, classified into pathotype group 3. 'akiriso', a clubroot-resistant f(1) cultivar, showed resistance to isolate no. 14. to increase the durability of resistance, we attempted to identify the cr locus in 'akiriso'. cr in 'akiriso' segregated as a single dominant gene and ...201223226089
the impact of genome triplication on tandem gene evolution in brassica rapa.whole genome duplication (wgd) and tandem duplication (td) are both important modes of gene expansion. however, how wgd influences tandemly duplicated genes is not well studied. we used brassica rapa, which has undergone an additional genome triplication (wgt) and shares a common ancestor with arabidopsis thaliana, arabidopsis lyrata, and thellungiella parvula, to investigate the impact of genome triplication on tandem gene evolution. we identified 2,137, 1,569, 1,751, and 1,135 tandem gene arra ...201223226149
construction of genetic linkage map and mapping of qtl for seed color in brassica rapa.a genetic linkage map of brassica rapa l. was constructed using recombinant inbred lines (rils) derived from a cross between yellow-seeded cultivar sampad and a yellowish brown seeded inbred line 3-0026.027. the rils were evaluated for seed color under three conditions: field plot, greenhouse, and controlled growth chambers. variation for seed color in the rils ranged from yellow, like yellow sarson, to dark brown/black even though neither parent had shown brown/black colored seeds. one major qt ...201223231600
[preliminary research of quality standard on brassica rapa].to explore the physical and behavioral, microscopic and chemical characteristic traits of brassica rapa, and supply scientific bases for establishing its quality control standard.201223234129
a triallelic genetic male sterility locus in brassica napus: an integrative strategy for its physical mapping and possible local chromosome evolution around it.spontaneous male sterility is an advantageous trait for both constructing efficient pollination control systems and for understanding the developmental process of the male reproductive unit in many crops. a triallelic genetic male-sterile locus (bnms5) has been identified in brassica napus; however, its complicated genome structure has greatly hampered the isolation of this locus. the aim of this study was to physically map bnms5 through an integrated map-based cloning strategy and analyse the l ...201323243189
metabolite profiling of mizuna ( brassica rapa l. var. nipponsinica) to evaluate the effects of organic matter amendments.organic matter amendment is an essential agricultural protocol to improve soil function and carbon sequestration. however, the effect of organic matter amendments on crop quality has not been well-defined. this study applied gas chromatography-mass spectrometry to investigate the metabolite profiling of mizuna ( brassica rapa l. var. nipponsinica) with different organic matter amendments with respect to quality and quantity. principal component analysis showed that 33.4, 15.6, and 6.6% of the to ...201323244647
functioning of metal contaminated garden soil after remediation.the effect of remediation using three edta doses (10, 30, 60 mmol kg(-1)) on soil functioning was assessed using column experiment and brassica rapa. soil washing removed up to 77, 29 and 72% of metals from soil contaminated with 1378, 578 and 8.5 mg kg(-1) of pb, zn and cd, respectively. sequential extraction indicated removal from the carbonate soil fraction. metal oral-accessibility from the stomach phase was reduced by up to 75 and from the small intestine by up to 79% (pb). part of metals ( ...201323246748
ogura-cms in chinese cabbage (brassica rapa ssp. pekinensis) causes delayed expression of many nuclear genes.we investigated the mechanism regulating cytoplasmic male sterility (cms) in brassica rapa ssp. pekinensis using floral bud transcriptome analyses of ogura-cms chinese cabbage and its maintainer line in b. rapa 300-k oligomeric probe (br300k) microarrays. ogura-cms chinese cabbage produced few and infertile pollen grains on indehiscent anthers. compared to the maintainer line, cms plants had shorter filaments and plant growth, and delayed flowering and pollen development. in microarray analysis, ...201323265314
effects of phytohormones and jasmonic acid on glucosinolate content in hairy root cultures of sinapis alba and brassica rapa.although some study have established hairy root cultures from brassicaceous plants with glucosinolates (gs) as characteristic secondary metabolite, studies are missing which compare hairy roots with the corresponding mother plants. therefore, two different plant species-sinapis alba and brassica rapa subsp. rapa pygmeae teltoviensis-were transformed with the agrobacterium rhizogenes strain a4. aliphatic and indolyl gs were present in b. rapa, exhibiting larger quantities in leaves than in roots. ...201323269631
inferring the brassica rapa interactome using protein-protein interaction data from arabidopsis thaliana.following successful completion of the brassica rapa sequencing project, the next step is to investigate functions of individual genes/proteins. for arabidopsis thaliana, large amounts of protein-protein interaction (ppi) data are available from the major ppi databases (dbs). it is known that brassica crop species are closely related to a. thaliana. this provides an opportunity to infer the b. rapa interactome using ppi data available from a. thaliana. in this paper, we present an inferred b. ra ...201223293649
qtl analysis using snp markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, raphanus sativus l.snp markers for qtl analysis of 4-mtb-gsl contents in radish roots were developed by determining nucleotide sequences of bulked pcr products using a next-generation sequencer. dna fragments were amplified from two radish lines by multiplex pcr with six primer pairs, and those amplified by 2,880 primer pairs were mixed and sequenced. by assembling sequence data, 1,953 snps in 750 dna fragments, 437 of which have been previously mapped in a linkage map, were identified. a linkage map of nine linka ...201323308250
transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line nr1 and nsa cms line in brassica napus.the fertile and sterile plants were derived from the self-pollinated offspring of the f1 hybrid between the novel restorer line nr1 and the nsa cms line in brassica napus. to elucidate gene expression and regulation caused by the a and c subgenomes of b. napus, as well as the alien chromosome and cytoplasm from sinapis arvensis during the development of young floral buds, we performed a genome-wide high-throughput transcriptomic sequencing for young floral buds of sterile and fertile plants.201323324545
randomized double-blind placebo-controlled trial of powdered brassica rapa ethanol extract on alteration of body composition and plasma lipid and adipocytokine profiles in overweight subjects.we evaluated the effects of brassica rapa ethanol extract (bree) on body composition and plasma lipid profiles through a randomized, double-blind, and placebo-controlled trial in overweight subjects. fifty-eight overweight participants (age 20-50 years, body mass index23.0-24.9) were randomly assigned to two groups and served bree (2 g/day) or placebo (starch, 2 g/day) for 10 weeks. body compositions, nutrients intake, plasma lipids, adipocytokines, and hepatotoxicity biomarkers were assessed in ...201323342969
analysis of animal and plant selenometabolites in roots of a selenium accumulator, brassica rapa var. peruviridis, by speciation.many studies have examined the metabolic pathway of selenium (se) compounds in se-accumulating plants (hereafter "se accumulators") when the plants are exposed to inorganic se, such as selenite and selenate. however, if we were to consider se circulation in the biosphere, the metabolism of organic se, in particular, selenometabolites of animals and plants, in plants should be elucidated. in this study, brassica rapa var. peruviridis, a known se accumulator, was hydroponically cultivated and then ...201323348393
genotypic variation of the glucosinolate profile in pak choi (brassica rapa ssp. chinensis).thirteen different pak choi (brassica rapa ssp. chinensis) cultivars were characterized regarding their glucosinolate profile analyzed by hplc-dad-ms. the identified glucosinolates were subjected to principal component analysis, and three distinct groups of pak choi sprouts were identified. group differences were marked mainly by variations in the aliphatic glucosinolate profile such as differing levels of 3-butenyl glucosinolate and 2-hydroxy-3-butenyl glucosinolate as well as by their varying ...201323350944
an experimental test of the transmission-virulence trade-off hypothesis in a plant virus.the transmission-virulence trade-off hypothesis is one of the few adaptive explanations of virulence evolution, and assumes that there is an overall positive correlation between parasite transmission and virulence. the shape of the transmission-virulence relationship predicts whether virulence should evolve toward either a maximum or to an intermediate optimum. a positive correlation between each of these traits and within-host growth is often suggested to underlie the relationship between virul ...201323356619
evolution of the onsen retrotransposon family activated upon heat stress in brassicaceae.a ty1/copia-like retrotransposon, onsen, is activated by heat stress in arabidopsis thaliana, and its de novo integrations that were observed preferentially within genes implies its regulation of neighboring genes. here we show that onsen related copies were found in most species of brassicaceae, forming a cluster with each species in phylogenetic tree. most copies were localized close to genes in arabidopsis lyrata and brassica rapa, suggesting conserved integration specificity of onsen family ...201323370337
nucleotide sequence variation of glabra1 contributing to phenotypic variation of leaf hairiness in brassicaceae vegetables.glabra1 (gl1) belongs to the group of r2r3-myb transcription factors and is known to be essential for trichome initiation in arabidopsis. in our previous study, we identified a gl1 ortholog in brassica rapa as a candidate for the gene controlling leaf hairiness by qtl analysis and suggested that a 5-bp deletion (b-allele) and a 2-bp deletion (d-allele) in the exon 3 of brgl1 and a non-synonymous snp (c-allele) in the second nucleotide of exon 3 possibly cause leaf hairlessness. in this study, we ...201323370467
genome-wide identification and analysis of the b3 superfamily of transcription factors in brassicaceae and major crop plants.the plant-specific b3 superfamily of transcription factors has diverse functions in plant growth and development. using a genome-wide domain analysis, we identified 92, 187, 58, 90, 81, 55, and 77 b3 transcription factor genes in the sequenced genome of arabidopsis, brassica rapa, castor bean (ricinus communis), cocoa (theobroma cacao), soybean (glycine max), maize (zea mays), and rice (oryza sativa), respectively. the b3 superfamily has substantially expanded during the evolution in eudicots pa ...201323377560
identification and characterization of crr1a, a gene for resistance to clubroot disease (plasmodiophora brassicae woronin) in brassica rapa l.clubroot disease, caused by the obligate biotrophic protist plasmodiophora brassicae woronin, is one of the most economically important diseases of brassica crops in the world. although many clubroot resistance (cr) loci have been identified through genetic analysis and qtl mapping, the molecular mechanisms of defense responses against p. brassicae remain unknown. fine mapping of the crr1 locus, which was originally identified as a single locus, revealed that it comprises two gene loci, crr1a an ...201323382954
the dispensable chromosome of leptosphaeria maculans shelters an effector gene conferring avirulence towards brassica rapa.phytopathogenic fungi frequently contain dispensable chromosomes, some of which contribute to host range or pathogenicity. in leptosphaeria maculans, the stem canker agent of oilseed rape (brassica napus), the minichromosome was previously suggested to be dispensable, without evidence for any role in pathogenicity. using genetic and genomic approaches, we investigated the inheritance and molecular determinant of an l. maculans-brassica rapa incompatible interaction. single gene control of the re ...201323406519
[transfer characteristics of cadmium in soil-vegetable-insect food chain].taking two kinds of vegetables (brassica rapa and amaranthus mangostanus) and one insect species (prodenia litura) as test materials, a greenhouse pot experiment was conducted to study the transfer characteristics of cadmium (cd) in soil-vegetable-insect food chain and the distribution patters of different cd chemical forms in the organs of the two vegetables. with the increasing concentration of applied cd in soil, the biomass of the two vegetables decreased significantly, while the cd concentr ...201223431799
a new phenanthrene derivative and two diarylheptanoids from the roots of brassica rapa ssp. campestris inhibit the growth of cancer cell lines and ldl-oxidation.brassica rapa ssp. campestris (brassicaceae) is a conical, deep purple, edible root vegetable commonly known as a turnip. we initiated phytochemical and pharmacological studies to search for biological active compounds from the roots of b. rapa ssp. campestris. we isolated a novel phenanthrene derivative, 6-methoxy-1-[10-methoxy-7-(3-methylbut-2-enyl)phenanthren-3-yl]undecane-2,4-dione, named brassicaphenanthrene a (3) along with two known diarylheptanoid compounds, 6-paradol (1) and trans-6-sho ...201323435947
interfamily transfer of dual nb-lrr genes confers resistance to multiple pathogens.a major class of disease resistance (r) genes which encode nucleotide binding and leucine rich repeat (nb-lrr) proteins have been used in traditional breeding programs for crop protection. however, it has been difficult to functionally transfer nb-lrr-type r genes in taxonomically distinct families. here we demonstrate that a pair of arabidopsis (brassicaceae) nb-lrr-type r genes, rps4 and rrs1, properly function in two other brassicaceae, brassica rapa and brassica napus, but also in two solana ...201323437080
a battery of bioassays for the evaluation of phenanthrene biotoxicity in soil.a battery of bioassays was used to assess the ecotoxicological risk of soil spiked with a range of phenanthrene levels (0.95, 6.29, 38.5, 58.7, 122, and 303 μg g(-1) dry soil) and aged for 69 days. multiple species (viz. brassica rapa, eisenia feotida, vibrio fischeri), representing different trophic levels, were used as bioindicator organisms. among acute toxicity assays tested, the v. fischeri luminescence inhibition assay was the most sensitive indicator of phenanthrene biotoxicity. more than ...201323440446
the fate of arabidopsis thaliana homeologous cnss and their motifs in the paleohexaploid brassica rapa.following polyploidy, duplicate genes are often deleted, and if they are not, then duplicate regulatory regions are sometimes lost. by what mechanism is this loss and what is the chance that such a loss removes function? to explore these questions, we followed individual arabidopsis thaliana-a. thaliana conserved noncoding sequences (cnss) into the brassica ancestor, through a paleohexaploidy and into brassica rapa. thus, a single brassicaceae cns has six potential orthologous positions in b. ra ...201323493633
growth and antioxidant response of brassica rapa var. rapa l. (turnip) irrigated with different compositions of paper and board mill (pbm) effluent.current study presents the effect of irrigation with different compositions (0%, 20%, 40%, 60%, 80% and 100%) of pbm effluent on growth and antioxidant potential of brassica rapa var. rapa l. plants. seeds were exposed to different pbm effluent compositions, which resulted in significant decrease in their germination potential with elevated delay index. significant changes in growth parameters (plant height, number of leaves and leaf area) were recorded for turnip plants at regular intervals (25 ...201323507497
carbohydrate derivatives from the roots of brassica rapa ssp. campestris and their effects on ros production and glutamate-induced cell death in ht-22 cells.phytochemical investigation of the roots of brassica rapa ssp. campestris led to the isolation of three new carbohydrate derivatives, namely sucrose 3,3',4'-triisovalerate (2), sucrose 6,3',4'-triisovalerate (3), and ethanone-1-c-β-d-glucopyranoside (3,7-anhydro-1-deoxy-d-glycero-d-gulo-2-octulose, 6), along with four known carbohydrate derivatives, 2,6,3',4'-tetraisovalerate (1), ethyl β-d-glucopyranoside (4), n-butyl β-d-fructofuranoside (5), and n-pentyl β-d-fructofuranoside (7), which were i ...201223507511
influence of stress hormones on the auxin homeostasis in brassica rapa seedlings.key message : stress hormones, particularly jasmonic acid, influenced root growth, auxin levels, and transcription of auxin amidohydrolase briar3 in brassica rapa seedlings, while auxin conjugate synthetases brgh3.1 and brgh3.9 were down-regulated by all treatments. the influence of stress hormones: jasmonic acid (ja), salicylic acid (sa), and abscisic acid (aba) on 1-day-old seedlings of chinese cabbage (brassica rapa l. ssp. pekinensis) was investigated with particular focus on auxin levels an ...201323508255
breaking restricted taxonomic functionality by dual resistance genes.nb-lrr-type disease resistance (r) genes have been used in traditional breeding programs for crop protection. however, functional transfer of nb-lrr-type r genes to plants in taxonomically distinct families to establish pathogen resistance has not been successful. here we demonstrate that a pair of arabidopsis (brassicaceae) nb-lrr-type r genes, rps4 and rrs1, properly function in two other brassicaceae, brassica rapa and b. napus, but also in two solanaceae, nicotiana benthamiana and tomato (so ...201323518587
evaluation of glucosinolate variation in a collection of turnip (brassica rapa) germplasm by the analysis of intact and desulfo glucosinolates.glucosinolates (gls) are secondary metabolites occurring in cruciferous species. these compounds are important for plant defense, human health, and the characteristic flavor of brassica vegetables. in this study, the gls in tubers from a collection of 48 turnip ( brassica rapa ) accessions from different geographic origin were analyzed. two different methods were used: desulfo gls were analyzed by high-performance liquid chromatography with a photodiode array detector, and intact gls were analyz ...201323528157
synthesis of indolyl-3-acetonitrile derivatives and their inhibitory effects on nitric oxide and pge2 productions in lps-induced raw 264.7 cells.arvelexin is one of major constituents of brassica rapa that exerts anti-inflammatory activities. several indolyl-3-acetonitrile derivatives were synthesized as arvelexin analogs and evaluated for their abilities to inhibit no and pge2 productions in lps-induced raw 264.7 cells. of the indolyl-3-acetonitriles synthesized, compound 2k, which possesses a hydroxyl group at c-7 position of the indole ring and an n-methyl substituent, more potently inhibited no and pge2 productions and was less cytot ...201323528295
homoeologous gsl-elong gene replacement for manipulation of aliphatic glucosinolates in brassica rapa l. by marker assisted selection.aliphatic glucosinolates are the predominant sulfur-rich plant secondary metabolites in economically important brassica crops. glucosinolates and their hydrolysis products are involved in plant-microbe, plant-insect, plant-animal, and plant-human interactions. it is, therefore, important to manipulate glucosinolate profiles and contents in brassica species. in this study, aliphatic glucosinolates were genetically manipulated through homoeologous recombination in backcross lines followed by marke ...201323532458
involvement of mlpk pathway in intraspecies unilateral incompatibility regulated by a single locus with stigma and pollen factors.plants have evolved many systems to prevent undesirable fertilization. among these, incompatibility is a well-organized system in which pollen germination or pollen tube growth is inhibited in pistils. we previously found that a novel one-way pollen-stigma incompatibility response [unilateral incompatibility (ui)] occurred between two self-incompatible brassica rapa plants, a turkish line, and a japanese cultivated hybrid variety, "osome." pollen from the turkish line is rejected on the stigma o ...201323550141
assessment of allelopathic properties of aloe ferox mill. on turnip, beetroot and carrot.turnip (brassica rapa var. rapa l.), beetroot (beta vulgaris l.) and carrot (daucus carota l.) are common vegetables in south africa. the allelopathic potential of aqueous leaf and root extracts of aloe ferox mill.- a highly valued medicinal plant- was evaluated against seed germination and seedling growth of the three vegetables in petri dish experiments. the extracts were tested at concentrations of 2, 4, 6, 8, and 10 mg/ml. leaf extract concentrations above 4 mg/ml inhibited the germination o ...201223558992
characterization and expression analysis of dirigent family genes related to stresses in brassica.the dirigent (dir) genes are playing a vital role in enhancing stress resistance in different crop plants. in this study, we collected 29 dir like genes, two from a brassica rapa cv. osome full length cdna library and 27 from the b. rapa database designated as b. rapa dirigent (brdir) like genes. sequence analysis and a comparison study of these genes confirmed that seven were dirigent and the remaining 22 were dirigent like genes. expression analysis revealed an organ specific expression of the ...201323562798
mapping quantitative trait loci for tissue culture response in vcs3m-dh population of brassica rapa.quantitative trait loci (qtl) controlling callus induction and plant regeneration were identified in the vcs3m-dh population of brassica rapa. quantitative trait loci (qtl) controlling callus induction and plant regeneration were identified in the vcs3m-dh population of brassica rapa. the vcs3m-dh population showed wide and continuous variation in callus induction and shoot regeneration. significant coefficient correlations were detected between these two parameters. broad-sense heritability (h ...201323563522
yield, chemical composition and nutritional quality responses of carrot, radish and turnip to elevated atmospheric carbon dioxide.future concentration of carbon dioxide in the atmosphere is very important due to its apparent economic and environmental impact in terms of climate change. however, a compressive assessment of its effect on the nutritional and chemical characteristics of food crops has yet to be established. in the present study the impact of elevated atmospheric co2 on the yield, chemical composition and nutritional quality of three root vegetables, carrot (daucus carota l. cv. t-1-111), radish (raphanus sativ ...201323576218
field evaluation of in situ remediation of cd-contaminated soil using four additives, two foliar fertilisers and two varieties of pakchoi.this study was conducted to determine the optimal planting mode for pakchoi (brassica rapa chinensis) in cd-contaminated soil to reduce the accumulation of cd in the edible parts while maintaining yields. four additives (red mud (rm), silicon calcium fertiliser (sc), spodium (sp) and calcium magnesium phosphate (cmp)), two foliar fertilisers (ca and zn) and two varieties of pakchoi (aijiaohuang (aj) and baixuegongzhu (bx)) were used in this study. the results show that the addition of sc and rm ...201323603772
a consensus map of rapeseed (brassica napus l.) based on diversity array technology markers: applications in genetic dissection of qualitative and quantitative traits.dense consensus genetic maps based on high-throughput genotyping platforms are valuable for making genetic gains in brassica napus through quantitative trait locus identification, efficient predictive molecular breeding, and map-based gene cloning. this report describes the construction of the first b. napus consensus map consisting of a 1,359 anchored array based genotyping platform; diversity arrays technology (dart), and non-dart markers from six populations originating from australia, canada ...201323617817
molecular characterization and phylogeny of a phytoplasma associated with phyllody disease of toria (brassica rapa l. subsp. dichotoma (roxb.)) in india.samples from toria plants (brassica rapa l. subsp. dichotoma (roxb.)) exhibiting phyllody, virescence, witches broom, extensive malformation of floral parts, formation of bladder like siliquae and flower sterility were collected from four different locations in india. sequencing and phylogenetic analysis of the 16s rrna, a part of 23s rrna, partial sec a genes, rp gene and 16s-23s intergenic spacer region indicated that the phytoplasmas associated with toria phyllody (tp) symptoms were identical ...201023637492
fine mapping of the clubroot resistance gene crb and development of a useful selectable marker in brassica rapa.in chinese cabbage (brassica rapa), the clubroot resistance (cr) gene crb is effective against plasmodiophora brassicae isolate no. 14, which is classified as pathotype group 3. although markers linked to crb have been reported, an accurate position in the genome and the gene structure are unknown. to determine the genomic location and estimate the structure of crb, we developed 28 markers (average distance, 20.4 kb) around crb and constructed a high-density partial map. the precise position of ...201323641188
time-lapse imaging of self- and cross-pollinations in brassica rapa.pollination is an important process in the life cycle of plants and is the first step in bringing together the male and female gametophytes for plant reproduction. while pollination has been studied for many years, accurate knowledge of the morphological aspects of this process is still far from complete. this study therefore focuses on a morphological characterization of pollination, using time-series image analysis of self- and cross-pollinations in brassica rapa.201323644359
reconstruction of the brassica rapa ancestral genome. 201323653469
deciphering the diploid ancestral genome of the mesohexaploid brassica rapa.the genus brassica includes several important agricultural and horticultural crops. their current genome structures were shaped by whole-genome triplication followed by extensive diploidization. the availability of several crucifer genome sequences, especially that of chinese cabbage (brassica rapa), enables study of the evolution of the mesohexaploid brassica genomes from their diploid progenitors. we reconstructed three ancestral subgenomes of b. rapa (n = 10) by comparing its whole-genome seq ...201323653472
a review of metal (pb and zn) sensitive and ph tolerant bioassay organisms for risk screening of metal-contaminated acidic soils.to improve risk estimates at the screening stage of ecological risk assessment (era), short duration bioassays tailored to undisturbed soil cores from the contaminated site could be useful. however, existing standardized bioassays use disturbed soil samples and often ph sensitive organisms. this is a problem as naturally acidic soils are widespread. changing soil properties to suit the test organism may change metal bioavailability, leading to erroneous risk estimates. for bioassays in undisturb ...201323688951
a large-scale introgression of genomic components of brassica rapa into b. napus by the bridge of hexaploid derived from hybridization between b. napus and b. oleracea.brassica rapa (aa) has been used to widen the genetic basis of b. napus (aacc), which is a new but important oilseed crop worldwide. in the present study, we have proposed a strategy to develop new type b. napus carrying genomic components of b. rapa by crossing b. rapa with hexaploid (aacccc) derived from b. napus and b. oleracea (cc). the hexaploid exhibited large flowers and high frequency of normal chromosome segregation, resulting in good seed set (average of 4.48 and 12.53 seeds per pod by ...201323699961
[identification and nucleotide polymorphisms in brassica rapa genes coding cold shock domain proteins (csdp)].full-length brcsdp2 and brcsdp4 cold shock gene sequences of brassica rapa are obtained. it is shown that the isolated genes belong to a group atcsp2/atcsp4 of arabidopsis thaliana and tscsdp2/tscsdp4 of thellungiella salsuginea genes encoding proteins with a cold shock domain (csd) and two zinc finger motives. the structure and the allelic variants of these genes are described and characterized. it is shown that the identified allelic polymorphism is due to both of point substitutions and small ...201323705499
the consequences of alternating diet on performance and food preferences of a specialist leaf beetle.the food quality of a given host plant tissue will influence the performance and may also affect the preference behavior of herbivorous animals. as nutrient contents and defense metabolite concentrations can vary significantly between different parts of a plant and change over time, herbivores are potentially confronted with diet differing in quality even when feeding on a single plant individual. here we investigated to what extent feeding exclusively either on young or old, mature leaves of br ...201323727303
reduction of leaf area and symptom severity as proxies of disease-induced plant mortality: the example of the cauliflower mosaic virus infecting two brassicaceae hosts.disease induced effects on host survival are important to understand the evolution of parasitic virulence and host resistance/tolerance. unfortunately, experiments evaluating such effects are in most cases logistically demanding justifying the measurement of survival proxies. for plant hosts commonly used proxies are leaf area and the nature and severity of visual qualitative disease symptoms. in this study we tested whether these traits are indeed correlated to the host mortality rate induced b ...201323742852
characterization of mutants of a highly cross-reactive calcium-binding protein from brassica pollen for allergen-specific immunotherapy.the major turnip (brassica rapa) pollen allergen, belongs to a family of calcium-binding proteins (i.e., two ef-hand proteins), which occur as highly cross-reactive allergens in pollen of weeds, grasses and trees. in this study, the ige binding capacity and allergenic activity of three recombinant allergen variants containing mutations in their calcium-binding sites were analyzed in sensitized patients with the aim to identify the most suitable hypoallergenic molecule for specific immunotherapy. ...201323790497
in vitro digestion/caco-2 cell model to estimate cadmium and lead bioaccessibility/bioavailability in two vegetables: the influence of cooking and additives.the estimation of heavy metal bioaccessibility and bioavailability in vegetables is helpful for human health risk assessment. using an in vitro digestion/caco-2 cell model, the bioaccessibility and bioavailability of cadmium (cd) and lead (pb) in raw/cooked pakchoi (brassica rapa l., chinensis group) and malabar spinach (basella rubra l.) were studied. the effect of the addition of iron, calcium and acetic acid to the samples was also determined. the results indicated that cd bioaccessibility wa ...201323791752
dna methylation alterations at 5'-ccgg sites in the interspecific and intraspecific hybridizations derived from brassica rapa and b. napus.dna methylation is an important regulatory mechanism for gene expression that involved in the biological processes of development and differentiation in plants. to investigate the association of dna methylation with heterosis in brassica, a set of intraspecific hybrids in brassica rapa and b. napus and interspecific hybrids between b. rapa and b. napus, together with parental lines, were used to monitor alterations in cytosine methylation at 5'-ccgg sites in seedlings and buds by methylation-sen ...201323824315
rna-seq based snps in some agronomically important oleiferous lines of brassica rapa and their use for genome-wide linkage mapping and specific-region fine mapping.brassica rapa (aa) contains very diverse forms which include oleiferous types and many vegetable types. genome sequence of b. rapa line chiifu (ssp. pekinensis), a leafy vegetable type, was published in 2011. using this knowledge, it is important to develop genomic resources for the oleiferous types of b. rapa. this will allow more involved molecular mapping, in-depth study of molecular mechanisms underlying important agronomic traits and introgression of traits from b. rapa to major oilseed cro ...201323837684
quantification of alternaria brassicicola infection in the arabidopsis thaliana and brassica rapa subsp. pekinensis.black spot caused by alternaria brassicicola is an important fungal disease affecting cruciferous crops, including korean cabbage (brassica rapa subsp. pekinensis). the interaction between arabidopsis thaliana and alt. brassicicola is a representative model system, and objective estimation of disease progression is indispensable for accurate functional analyses. five strains caused black spot symptom progression on korean cabbage and ara. thaliana ecotype col-0. in particular, challenge with the ...201323842466
uptake of arsenic species by turnip (brassica rapa l.) and lettuce (lactuca sativa l.) treated with roxarsone and its metabolites in chicken manure.roxarsone is an organoarsenic feed additive that can be metabolised to other higher toxic arsenic (as) species in animal manure such as arsenate, arsenite, monomethylarsonic acid, dimethylarsinic acid, 3-amino-4-hydroxyphenylarsonic acid and other unknown as species. the accumulation, transport and distribution of as species in turnip (brassica rapa l.) and lettuce (lactuca sativa l.) amended with roxarsone and its metabolites in chicken manure were investigated. results showed arsenite was the ...201323859781
comparative mapping of raphanus sativus genome using brassica markers and quantitative trait loci analysis for the fusarium wilt resistance trait.fusarium wilt (fw), caused by the soil-borne fungal pathogen fusarium oxysporum is a serious disease in cruciferous plants, including the radish (raphanus sativus). to identify quantitative trait loci (qtl) or gene(s) conferring resistance to fw, we constructed a genetic map of r. sativus using an f2 mapping population derived by crossing the inbred lines '835' (susceptible) and 'b2' (resistant). a total of 220 markers distributed in 9 linkage groups (lgs) were mapped in the raphanus genome, cov ...201323864230
shifts in the evolutionary rate and intensity of purifying selection between two brassica genomes revealed by analyses of orthologous transposons and relics of a whole genome triplication.recent sequencing of the brassica rapa and brassica oleracea genomes revealed extremely contrasting genomic features such as the abundance and distribution of transposable elements between the two genomes. however, whether and how these structural differentiations may have influenced the evolutionary rates of the two genomes since their split from a common ancestor are unknown. here, we investigated and compared the rates of nucleotide substitution between two long terminal repeats (ltrs) of ind ...201323869625
induced production of 1-methoxy-indol-3-ylmethyl glucosinolate by jasmonic acid and methyl jasmonate in sprouts and leaves of pak choi (brassica rapa ssp. chinensis).pak choi plants (brassica rapa ssp. chinensis) were treated with different signaling molecules methyl jasmonate, jasmonic acid, linolenic acid, and methyl salicylate and were analyzed for specific changes in their glucosinolate profile. glucosinolate levels were quantified using hplc-dad-uv, with focus on induction of indole glucosinolates and special emphasis on 1-methoxy-indol-3-ylmethyl glucosinolate. furthermore, the effects of the different signaling molecules on indole glucosinolate accumu ...201323873294
myb transcription factors regulate glucosinolate biosynthesis in different organs of chinese cabbage (brassica rapa ssp. pekinensis).in this study, we investigated the expression of seven myb transcription factors (a total of 17 genes that included dof1.1, iqd1-1, myb28, myb29, myb34, myb51, and myb122 and their isoforms) involved in aliphatic and indolic glucosinolate (gsl) biosynthesis and analyzed the aliphatic and indolic gsl content in different organs of chinese cabbage (brassica rapassp. pekinensis). myb28 and myb29 expression in the stem was dramatically different when compared with the levels in the other organs. myb ...201323881053
genetic analysis of health-related secondary metabolites in a brassica rapa recombinant inbred line population.the genetic basis of the wide variation for nutritional traits in brassica rapa is largely unknown. a new recombinant inbred line (ril) population was profiled using high performance liquid chromatography (hplc) and nuclear magnetic resonance (nmr) analysis to detect quantitative trait loci (qtls) controlling seed tocopherol and seedling metabolite concentrations. ril population parent l58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate ...201323892600
gene ontology based characterization of expressed sequence tags (ests) of brassica rapa cv. osome.chinese cabbage (brassica rapa) is widely recognized for its economic importance and contribution to human nutrition but abiotic and biotic stresses are main obstacle for its quality, nutritional status and production. in this study, 3,429 express sequence tag (est) sequences were generated from b. rapa cv. osome cdna library and the unique transcripts were classified functionally using a gene ontology (go) hierarchy, kyoto encyclopedia of genes and genomes (kegg). kegg orthology and the structu ...201323898551
changes in the relative abundance and movement of insect pollinators during the flowering cycle of brassica rapa crops: implications for gene flow.the potential movement of transgenes from genetically modified crops to non-genetically modified crops via insect-mediated pollen dispersal has been highlighted as one of the areas of greatest concern in regards to genetically modified crops. pollen movement depends sensitively on spatial and temporal variation in the movement of insect pollinators between crop fields. this study tested the degree of variation in the diversity and relative abundance of flower-visiting insects entering versus lea ...201323937538
[transposon expression and potential effects on gene regulation of brassica rapa and b. oleracea genomes].transposons or transposable elements (tes) are ubiquitous and most abundant dna components in higher eukaryotes. recent sequencing of the brassica rapa and b. oleracea genomes revealed that the amplification of tes is one of the main factors inducing the difference in genome size. however, the expressions of tes and the te effects on gene regulation and functions of these two brassica diploid species were unclear. here, we analyzed the rna sequencing data of leaves, roots, and stems from b. rapa ...201323956090
genome-wide analysis of the ap2/erf transcription factor superfamily in chinese cabbage (brassica rapa ssp. pekinensis).chinese cabbage (brassica rapa ssp. pekinensis) is a member of one of the most important leaf vegetables grown worldwide, which has experienced thousands of years in cultivation and artificial selection. the entire chinese cabbage genome sequence, and more than forty thousand proteins have been obtained to date. the genome has undergone triplication events since its divergence from arabidopsis thaliana (13 to 17 mya), however a high degree of sequence similarity and conserved genome structure re ...201323972083
consequences of gene flow between oilseed rape (brassica napus) and its relatives.numerous studies have focused on the probability of occurrence of gene flow between transgenic crops and their wild relatives and the likelihood of transgene escape, which should be assessed before the commercial release of transgenic crops. this review paper focuses on this issue for oilseed rape, brassica napus l., a species that produces huge numbers of pollen grains and seeds. we analyze separately the distinct steps of gene flow: (1) pollen and seeds as vectors of gene flow; (2) spontaneous ...201323987810
protective effects of 6-hydroxy-1-methylindole-3-acetonitrile on cisplatin-induced oxidative nephrotoxicity via nrf2 inactivation.we previously demonstrated the ethanol extract of the roots of brassica rapa protects against cisplatin-induced nephrotoxicity by attenuating oxidative stress. here, we investigated the nephroprotective effects of 6-hydroxy-1-methylindole-3-acetonitrile (6-hma), which was isolated from the roots of b. rapa, on cisplatin-induced toxicity in renal epithelial llc-pk1 cells and in rats with acute renal injury. pretreatment of llc-pk1 cells with 6-hma ameliorated cisplatin-induced cytotoxicity caused ...201323989062
transfer of auxinic herbicide resistance from brassica kaber to brassica juncea and brassica rapa through embryo rescue.auxinic herbicides are widely used in agriculture to selectively control broadleaf weeds. prolonged use of auxinic herbicides has resulted in the evolution of resistance to these herbicides in some biotypes of brassica kaber (wild mustard), a common weed in agricultural crops. in this study, auxinic herbicide resistance from b. kaber was transferred to brassica juncea and brassica rapa, two commercially important brassica crops, by traditional breeding coupled with in vitro embryo rescue. a high ...201323990700
toxicity of tributyltin (tbt) to terrestrial organisms and its species sensitivity distribution.the contamination of the terrestrial environment by disposal of tributyltin (tbt) by contaminated harbour sediments, sewage sludge and/or biocide products has been raising concerns and it may pose a risk to soil invertebrates and plants. this study aimed to improve the amount and quality of data for tbt toxicity in soils in order to assess the ecological risk of tbt to the terrestrial ecosystems. for this, bioassays were performed with the species porcellionides pruinosus, folsomia candida, bras ...201423994735
genome-wide characterization of the pectate lyase-like (pll) genes in brassica rapa.pectate lyases (pl) depolymerize demethylated pectin (pectate, ec 4.2.2.2) by catalyzing the eliminative cleavage of α-1,4-glycosidic linked galacturonan. pectate lyase-like (pll) genes are one of the largest and most complex families in plants. however, studies on the phylogeny, gene structure, and expression of pll genes are limited. to understand the potential functions of pll genes in plants, we characterized their intron-exon structure, phylogenetic relationships, and protein structures, an ...201323999922
identification, expression, and comparative genomic analysis of the ipt and ckx gene families in chinese cabbage (brassica rapa ssp. pekinensis).cytokinins (cks) have significant roles in various aspects of plant growth and development, and they are also involved in plant stress adaptations. the fine-tuning of the controlled ck levels in individual tissues, cells, and organelles is properly maintained by isopentenyl transferases (ipts) and cytokinin oxidase/dehydrogenases (ckxs). chinese cabbage is one of the most economically important vegetable crops worldwide. the whole genome sequencing of brassica rapa enables us to perform the geno ...201324001366
[identification of causative agent of brassica rapa bacterial diseases based on fatty acid composition of cellular lipids].the fatty acid composition of cell lipids of 15 strains isolated from the affected plants of rape and five collection strains has been studied. according to the results of chemotaxonomic analysis it has been found that 9 isolated strains are similar to representatives of species pseudomonas marginalis and pseudomonas fluorescens, and 6 - to those of xanthomonas campestris pv. campestris. the authors have established the efficiency of certain methods for the extraction of fatty acids used for the ...201324006784
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