Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [lipid component of streptomyces levoris membranes]. | the total composition of membranes is characterized in streptomyces levoris producing the polyene antibiotic levorin in the course of its growth; the lipid component has been studied in detail. the membranes contained protein (50--55%), lipids (37--40%), carbohydrates (3--4%), and rna (2--3%). the ratio between the main components hardly changed with age and only the content of total phosphorus in the membranes decreased noticeably. the lipid component was represented mainly by polar lipids (80- ... | 1979 | 481276 |
| [dynamics of the accumulation of adifferentiated variants in a submerged culture of streptomyces roseoflavus var. roseofungini]. | when streptomyces roseoflavus var. roseofungini is grown as a submerged culture in a chemically defined medium with stirring, it yields a number of variants in which the capacity to form the aerial mycelium is disturbed. inoculations of the submerged mycelium of different age into a solid medium give rise to both "tomentose" variants (tv) with the aerial mycelium and "naked" variants (nv) lacking the aerial mycelium. the latter variants comprise nocardia-like variants (nlv) with the fragmenting ... | 1979 | 481283 |
| [methodological approaches to developing a method of regulated antibiotic biosynthesis]. | methodologic approaches to the development of methods for controlled biosynthesis of antibiotics are discussed with reference to oxytetracycline biosynthesis. the method of acute experiments in conjunction with mathematical methods of experiment planning was used for determination of the substrate concentrations optimal for the culture growth and biosynthesis of oxytetracycline. it was found that such mathematic procedure provided simultaneous determination of optimal concentrations of all the s ... | 1979 | 485104 |
| [formation of antibiotic 2562 by a culture of the actinomycete, streptomyces griseovariabilis]. | an actinomycetous culture 2562 inhibiting the growth of gramnegative bacteria was isolated from a soil sample. the culture was classified as streptomyces griseovariabilis. it was found that culture 2562 produced an antibiotic belonging to the group of novobiocin. it consists of 2 components. one of them is identical to chlorobiocin and the other is a minor component of this group. some parameters of the antibiotic complex production by strain 2562 under submerged conditions were studied. nutrien ... | 1979 | 485116 |
| [effect of inorganic phosphate on levorin biosynthesis and on the mycelial makeup of streptomyces levoris]. | the effect of inorganic phosphate on biosynthesis of the polyenic antibiotic levorin by streptomyces levoris and composition of the culture mycelium was studied. it was found that the synthetic medium with 0.4 mm of phosphate was optimal for growth of str. levoris. when the concentration of phosphate was higher, the biomass increased, while the synthesis of levorin appeared to be inhibited and morphological changes in the culture were observed. phosphate had a significant effect on the mycelium ... | 1979 | 485117 |
| [metabolic products of microorganisms. 175. tetracenomycin c (author's transl)]. | streptomyces glaucescens, strain tü 49 = eth 22794, produces hydroxystreptomycin as well as the tetracenomycins, a mixture of several lipophilic antibiotics. the main component and the most active one is tetracenomycin c. tetracenomycin c has a molecular formula c23h20o11 and is chemically related to tetracyclines and anthracyclinones. the pale yellow antibiotic is active against some gram-positive bacteria, especially against streptomycetes. gram-negative bacteria and fungi are not inhibited. i ... | 1979 | 485765 |
| sinefungin, a potent inhibitor or s-adenosylmethionine: protein o-methyltransferase. | 1979 | 486211 | |
| [partial characterization of 2 extracellular aminopeptidases (arylamidases) from the dermatophyte keratinomyces ajelloi]. | two aminopeptidases ap1 and ap2 have been isolated from keratinomyces ajelloi filtrates. the molecular weight is about 27 000 for ap1 and 23 000 for ap2. both aminopeptidases present maximum activity at ph 9.35 but 50 p. 100 of maximum activity is observed between ph 7.5 and ph 8.5. km values measured at ph 9.35 with l-leucine-p-nitroanilide as substrate are 0.38 x 10(-3) m for ap1 and 0.43 x 10(-3) m for ap2. kcat at the same ph are 63.6 sec.-1 for ap-1 and 62.8 sec-1 for ap2. both aminopeptida ... | 1979 | 486581 |
| purification and properties of endo-beta-n-acetylglucosaminidase l from streptomyces plicatus. | an enzyme, previously described as endo-beta-n-acetylglucosaminidase l (tarentino, a.l., and maley, f. (1974) j. biol. chem. 249, 811-817) because of its apparent specificity for man(glcnac)2asn, has been purified to homogeneity. the enzyme has now been found to hydrolyze (glcnac)3 to (glcnac)2 plus glcnac, and (glcnac)4 to 2(glcnac)2, at twice the rate observed for man(glcnac)2asn. removal of the asparagine from the latter compound reduces the rate of hydrolysis by about 30-fold. reduction of ( ... | 1979 | 489563 |
| preliminary crystallographic data for lysozyme produced by streptomyces erythraeus. | 1979 | 490649 | |
| [biosynthesis of hyaluronic acid in parietal pleura of the rabbit (author's transl)]. | the parietal pleura of rabbits was incubated with 14c-glucosamine. it was found that 14c-glucosamine was incorporated into the fraction of crude glycosaminoglycans. then the crude glycosaminoglycans were fractionated by using specific mucopolysaccharide-lyases (hyaluronidase from streptomyces hyalurolytics, chondroitinase ac and chondroitinase abc). as a result, evidence was obtained that hyaluronic acid was synthesized in parietal pleura and was released into the surroundings. | 1979 | 494696 |
| investigations into rhizosphere microflora of some plants in libya. | the present investigation deals with the effect of raising hordeum vulgare and arachis hypogaea at two different stages on the microbial rhizosphere population. the studies were extended to reveal the microbiological occurrence in different horizons of a soil profile corresponding in length to root regions. bacteria were most abundant, followed by actinomycetes while fungi were less abundant and more restricted in their distribution. the three groups of microorganisms varied markedly in the rhiz ... | 1979 | 494853 |
| [action of mitomycin c on the variability of actinomyces hygroscopicus in forming a proteolytic complex of hygrolytin enzymes and the antibiotic, hygromycin b]. | the lethal and mutagenic effect of mitomycin c in doses of 10 and 15 micrograms/ml on the spores and 24-hour culture of act. hygroscopicus, strain o878 producing hygrolytin, a proteolytic enzyme and hygromycin b, an antibiotic was studied. it was found that mitomycin c had a high lethal effect on the organism. the lethal effect of the antibiotic depended on the stage of the culture development, mitomycin c dose and exposure time. the 24-hour culture was most sensitive to the effect of mitomycin ... | 1979 | 496416 |
| antitumor anthracycline antibiotics, aclacinomycin a and analogues. i. taxonomy, production, isolation and physicochemical properties. | aclacinomycin a and b, two major components of a new antitumor antibiotic complex, and their 19 analogues were produced by a culture of strain no. ma144-m1, which was identified as streptomyces galilaeus. they were isolated by chelation with copper ion and silicic acid chromatography, and characterized by physicochemical methods in the anthracycline group of antibiotics. | 1979 | 500500 |
| fertility properties and regulation of antimicrobial substance production by plasmid scp2 of streptomyces coelicolor. | streptomyces coelicolor a3(2) possesses two plasmids (scp1 and scp2) that act as sex factors. the plasmid deoxyribonucleic acid isolated from s. coelicolor a3(2) scp1- strains a617 and a585 had the same molecular weight and endonuclease cleavage pattern as the scp2 plasmid. the plasmidless strain s18 scp2- was isolated from the a617 x a585 cross. scp2 plasmid-containing strains acted as donors of chromosomal markers, whereas the plasmidless strain acted as recipient. the transfer of scp2+ donor ... | 1979 | 500559 |
| glycoprotein nature of yeast alkaline phosphatase. formation of active enzyme in the presence of tunicamycin. | the nonspecific alkaline phosphatase of yeast (saccharomyces strain 1710) has been purified by ion exchange, hydrophobic, and affinity chromatography. this vacuolar enzyme has a molecular weight of 130,000 and is composed of subunits (probably of 66,000 molecular weight). it also has a small quantity of covalently associated carbohydrate; hydrolysis yielded mannose and glucosamine. the endo-beta-n-acetylglucosaminidase of streptomyces plicatus released carbohydrate indicating that the latter was ... | 1979 | 500684 |
| herbicidins f and g, two new nucleoside antibiotics. | a mutant of streptomyces saganonensis no. 4075, obtained with n-methyl-n'-nitro-n-nitrosoguanidine treatment, produced herbicidins f and g without herbicidins a and b. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh. herbicidin f was obtained as colorless needles after extraction of the eluate using methylene dichloride. purification of herbicidin g was completed with silica-gel chromatography to give a crystalline powder. phys ... | 1979 | 511776 |
| conglobatin, a novel macrolide dilactone from streptomyces conglobatus atcc 31005. | fermentation of deposited cultures of streptomyces conglobatus, known to produce the polyether antibiotic, ionomycin has resulted in the isolation and characterization of a second metabolite, conglobatin (c28h38n2o6). x-ray analysis revealed a dimeric macrolide dilactone structure for conglobatin, similar to the structures of the mold metabolites vermiculin and pyrenophorin, from which the absolute configuration of conglobatin has been inferred. the dimer consists of two molecules of 7-hydroxy-8 ... | 1979 | 511778 |
| structures of olivanic acid derivatives mm 22380, mm 22381, mm 22382 and mm 22383; four new antibiotics isolated from streptomyces olivaceus. | 1979 | 511786 | |
| new aminoglycoside antibiotics, istamycins a and b. | 1979 | 511787 | |
| glaucescin, a bacteriocin-like substance from streptomyces glaucescens. | streptomyces glaucescens eth 22794 produced a variety of antibiotic substances. besides low molecular weight antibiotics like hydroxystreptomycin and the tetracenomycins, this strain excreted glaucescin, a high molecular weight product with bacteriocin-like properties. in plate tests the antagonism of glaucescin against streptomyces canadiensis was masked by the large inhibition zone caused by the tetracenomycins. glaucescin activity was revealed when a tetracenomycin-resistant mutant of s. cana ... | 1979 | 512635 |
| microbial transformations and 13c-nmr analysis of colchicine. | several microorganisms were screened for their ability to biotransform colchicine, and two were selected for preparative scale fermentations. streptomyces spectabilis and streptomyces griseus both produced o2-demethylcolchicine and o3-demethylcolchicine but in different amounts. the 13c-nmr assignments of colchicine, o10-demethylcolchicine, and trimethylcolchicinic acid are reported and were used to help identify the structures of the metabolites. | 1979 | 512852 |
| crystal structure of a bacterial protein proteinase inhibitor (streptomyces subtilisin inhibitor) at 2.6 a resolution. | 1979 | 513131 | |
| crystal structure of the complex of subtilisin bpn' with its protein inhibitor streptomyces subtilisin inhibitor. the structure at 4.3 angstroms resolution. | 1979 | 513133 | |
| in vitro oxidation of vitamin d metabolites of steroid dehydrogenases. | 1979 | 513739 | |
| [systematic position of an actinomycete producer of carotenoid pigments]. | 1979 | 514108 | |
| [uv-sensitive mutants of streptomyces olivaceus vkx]. | 14 uv-sensitive (uvs) mutants of s. olivaceus vkx isolated by replica-plating technique among 45780 survivors after the induction of mutations nitrosoguanidine or uv (about 0.03%). the sensitivity of uvs-mutants to uv-irradiation was 5-10 times higher than in the initial strains and in 4 uvs-mutants it was 100 and more times higher. one of the mutants was supersensitive to uv radiation. | 1979 | 516163 |
| [actinomyces rimosus resistance to oxytetracycline]. | high frequency of spontaneous and uv-and acridine dye-induced variants susceptible to oxytetracycline (otc) and deprived of the capacity for synthesizing this antibiotic was observed in strain lst-118 of actinomyces rimosus. the cells of strain lst-118 of act. rimosus contained extrachromosomal dna not found in its otc susceptible variant bs87, which provides evidence in favour of participation of the extrachromosomal genetic elements in control of otc resistance of the cells of act. rimosus, ls ... | 1979 | 518047 |
| [effect of lincomycin on its own producer, actinomyces roseolus, in periodic cultivation in a liquid medium]. | lincomycin added to the cultivation medium induced a number of changes in the organism producing it during its ontogenesis when grown recurrently on liquid media. it was found that lincomycin inhibited the culture growth and decreased the absolute amount of the antibiotic synthesized while the specific activity of the culture increased. a number of cytomorphological rearrangements relevant to the adaptive protective reactions was found. it is suggested that an increase in the resistance of the c ... | 1979 | 518048 |
| [effect of the products of the vital activity of yeast-like fungi on levorin synthesis]. | the effect on levorin synthesis of the cells and fermentation broth filtrates of candida tropicalis after their cultivation in the fermentation medium was studied. it was found that the yeast-like fungi belonging to candida excreted during their development some products capable of stimulating the synthesis of levorin by 40--60 per cent. when the actinomycete producing levorin was grown on the medium containing 80 per cent of the filtrate the level of levorin synthesis was the same as that obser ... | 1979 | 518050 |
| asymmetric manipulation of the membrane lipid bilayer of intact human erythrocytes with phospholipase a, c, or d induces a change in cell shape. | changes in the membrane morphology and phospholipid content of human erythrocytes were determined after incubation of intact cells with each of various exogeneous phospholipases (plases). plase a2 from naja naja or bee venom induced crenation of the cells in parallel with hydrolysis of the membrane phosphatidylcholine (pc). this crenated cell shape was reversed to a biconcave disc or cup-like form by a further treatment with lysophospholipase. in contrast, bacterial plase c from clostridium perf ... | 1979 | 521437 |
| genetics of actinorhodin biosynthesis by streptomyces coelicolor a3(2). | a series of 76 mutants of streptomyces coelicolor a3(2) specifically blocked in the synthesis of the binaphthoquinone antibiotic actinorhodin were classified into seven phenotypic classes on the basis of antibiotic activity, accumulation of pigmented precursors or shunt products of actinorhodin biosynthesis, and cosynthesis of actinorhodin in pairwise combinations of mutants. the polarity of cosynthetic reactions, and other phenotypic properties, allowed six of the mutant classes to be arranged ... | 1979 | 521794 |
| biosynthesis of kitasamycin (leucomycin) by leucine analog-resistant mutants of streptomyces kitasatoensis. | the biosynthesis of kitasamycin in streptomyces kitasatoensis b-896 was profoundly influenced by the addition of precursors to complex and defined media: l-valine and l-leucine directed biosynthesis towards the pairs a(4)/a(5) (r(2) = butyryl) and a(1)/a(3) (r(2) = isovaleryl), respectively, and total kitasamycin titers were doubled and quadrupled, respectively. s. kitasatoensis b-896 was very resistant (>20 mg/ml) to alpha-aminobutyric acid, an analog of l-valine, but very susceptible to l-leuc ... | 1979 | 525992 |
| presence of eta-isorhodomycinone in streptomyces galilaeus ja 3043. | 1979 | 527903 | |
| growth and production of anthracyclines in wild-type and mutant strains of streptomyces galilaeus. | the course of growth curves with respect to the biosynthesis of anthracyclines was followed in the wild low-producing strain streptomyces galilaeus ja 3043 and in its mutants g-167 (producing increased quantities of glycosides of epsilon-pyrromycinone) and j-14 (accumulating free epsilon-pyrromycinone). a two-phase type of fermentation (growth phase, production phase) was observed in strains ja 3043 and j-14. the maximum production of anthracyclines occurred only after the end of intense growth ... | 1979 | 527904 |
| effect of aeration efficiency and carbon source on the production of anthracyclines in streptomyces galilaeus. | biosynthesis of anthracyclines in streptomyces galilaeus during submerged cultivation is considerably influenced by aeration and by the concentration of glucose in the medium. at higher values of oxygen absorption rate both the production of epsilon-pyrromycinone glycosides in the wild strain ja 3043 and its production mutant g-167 and accumulation of free epsilon-pyrromycinone in the blocked mutant g-162 were found to be higher; the production of 7-deoxyaglycones was lower in all strains. the s ... | 1979 | 527905 |
| nitrosoguanidine-induced mutations in streptomyces indicus. | the mutagenic activities of five 1-alkyl derivatives of 3-nitro-1-nitrosoguanidine, viz. methyl, ethyl, propyl, isopentyl and octyl, were tested on streptomyces indicus. all the compounds were found to be mutagenic. the methyl and ethyl derivatives were most effective regarding mutational frequency. the effectiveness decreased with increasing number of c-atoms in the alkyl group of the mutagens. the compounds tested had a much higher mutagenic efficiency that uv-rays. a marked variation existed ... | 1979 | 527906 |
| controlled biosynthesis of neoviridogriseins, new homologues of viridogrisein. iii. production, structures and biological properties of neoviridogriseins i and iii. | the high response of our isolate of streptomyces griseoviridus was exploited to provoke the synthesis of new viridogrisein homologues by adding various amino acids to the culture medium in an attempt to replace the alanine, sarcosine, leucine and phenylsarcosine moieties of virdogrisein. among the amino acids added, l- and dl-alpha-amino-n-butyric acid and l-methionine gave new tlc spots which we named neoviridogriseins i and iii and neoviridogrisein vii, respectively. the structures of neovirid ... | 1979 | 528360 |
| production of 9-beta-d-arabinofuranosyladenine by a new species of streptomyces and its herbicidal activity. | 9-beta-d-arabinofuranosyladenine (ara-a) was produced by a new species of streptomyces designated as s. herbaceus. ara-a was found to have potent herbicidal activity against echinochloa crus-galli, digitaria adscendens and chenopodium ficifolium by the treatment with ara-a before germination of these plants. however, oryza sativa had strong resistance to ara-a. | 1979 | 528365 |
| bioconversion and biosynthesis of 16-membered macrolide antibiotics. xv. final steps in the biosynthesis of leucomycins. | 1979 | 528366 | |
| the detection of a plasmid in streptomyces ambofaciens ka-1028 and its possible involvement in spiramycin production. | 1979 | 528367 | |
| new aminoglycoside antibiotics, sannamycin. | 1979 | 528368 | |
| an inhibitor of chloramphenicol acetyltransferase produced by streptomyces. | 1979 | 528389 | |
| studies on actinomycetales producing antibiotics only on agar culture. i. screening, taxonomy and morphology-productivity relationship of streptomyces halstedii, strain sf-1993. | soil actinomycetales that produce antibiotics during growth on agar but not in submerged culture were searched for and about 25 strains were obtained. one of these, streptomyces halstedii, strain sf-1993, which produces an antibiotic designated sf-1993 was studied taxonomically and morphologically. the antibiotic-productivity of strain sf-1993 was correlated with mycelial morphology. the vegetative mycelium was filamentous in antibiotic-producing agar cultures, but fragmented in non-producing su ... | 1979 | 528390 |
| studies on actinomycetales producing antibiotics only on agar culture. ii. isolation, structure and biological properties of n-carbamoyl-d-glucosamine (substance sf-1993). | n-carbamoyl-d-glucosamine was isolated from the culture of streptomyces halstedii sf-1993. it showed weak antimicrobial activity against some gram-negative bacteria and some fungi. | 1979 | 528391 |
| structure of nanaomycin e, a new nanaomycin. | a new component, nanaomycin e, has been isolated from the culture broth of streptomyces rosa var, notoensis, which had been found to produce nanaomycins a, b, c and d. nanaomycin e was an epoxy derivative of nanaomycin a and was converted into nanaomycin a and 4a-epi-nanaomycin b by treatment with sodium hydrosulfite in an acidic aqueous solution. 4a-epi-nanaomycin b was quantiatively converted into nanaomycin a under alkaline conditions. | 1979 | 528392 |
| enzymatic conversion of aclacinomycin a to y by a specific oxidoreductase in streptomyces. | a specific oxidoreductase converting aclacinomycin a to a new analog, aclacinomycin y, was purified to apparent homogeneity from the culture filtrate of aclacinomycin-producing microorganisms. the isolated enzyme was a weakly acidic protein (isoelectric point, 5.9) with a molecular weight of about 72,000. the enzymatic reaction requires molecular oxygen and has a ph optimum at 5.5. the enzyme catalyzed an oxidation of the terminal sugar, l-cinerulose, of the trisaccharide moiety of aclacinomycin ... | 1979 | 528393 |
| identification of nosiheptide in feeds and detection of residues in animal tissues. | nosiheptic is determined in fermentation broths of streptomyces actuosus either by a microbiological method using staphylococcus aureus or, more easily, by an automated colorimetric method. the results obtained with both methods correspond well for concentrations greater than 100 microgram/ml with a standard deviation of 1-3%. for determination of nosiheptide as a feed additive, the microbiological assay is made more specific by pretreatment with petroleum ether and 1n hcl. standard deviation is ... | 1979 | 528472 |
| isolation and properties of spiramycin i 3-hydroxyl acylase from streptomyces and ambofaciens. | an enzyme preparation able to acylate the hydroxyl group at c-3 of the lactone ring of spiramycin was obtained from the spiramycin-producing strain, streptomyces ambofaciens isp-5053. the enzyme was purified about 33-fold from the crude extract by means of ammonium sulfate fractionation, diethylaminoethyl (deae) cellulose batchwise elution and deae cellulose column chromatography. the optimum ph for the enzyme activity was 8.5. the enzyme was activated by ca2++, mg2+, and mn2+ in this order, but ... | 1979 | 528537 |
| structure of streptomyces erythraeus lysozyme at 6 a resolution. | a 6 a resolution electron density map has been calculated for a bacterial lysozyme produced by streptomyces erythraeus. this lysozyme differs from the vertebrate lysozyme in its size, amino acid composition, and specificity. the structure was determined by the method of isomorphous replacement. three heavy atom derivatives were obtained by soaking crystals of the lysozyme in hgcl2, k2ptcl4, and uo2(no3)26h2o. the resulting electron density map clearly shows the molecular boundary. the molecule i ... | 1979 | 528538 |
| rapid differentiation of streptomyces from nocardia by liquid chromatography. | a liquid chromatographic method is described for analysis of aerobic actinomycetes for isomers of diaminopimelic acid. one or two colonies of organism were hydrolyzed with 6.0 mol of hcl per liter at 121 degrees c for 15 min. the hydrolysate was neutralized and buffered with an naoh solution (3 mol/liter) containing 0.15 mol of sodium borate per liter. precolumn derivatization with dansyl chloride was used to form a fluorescent product for detection. analysis was performed by reversed-phase, ion ... | 1979 | 528683 |
| the role of extracellular matrix in the formation of the sclerotome. | the development of the sclerotome is considered as a model for the formation of mesenchyme from an epithelium. in early epithelial somites, transmission and scanning electron microscopy indicate considerable ultrastructural similarity between the future sclerotome and dermamyotomal regions. subsequently, these two regions diverge in their development. in the forming dermamyotome, junctional complexes become more extensive and the cells become elongated, closely applied to each other, and have an ... | 1979 | 528873 |
| liberation of protoplasts from the yeast-like form of paracoccidioides brasiliensis by enzymatic treatment. | spherical and osmotically sensitive protoplasts were released from cultures of the yeast-like form of paracoccidioides basilienisis strain ivic pb9 through the action of a mixture of crude enzyme preparations: alpha and beta-glucanases and chitinase, obtained from culture filtrates of cladosporium resinae, basidiomycete qm 806 and streptomyces sp respectively. the highest efficiency of protoplast liberation was achieved when each crude enzyme preparation was used at 1 mg/ml. | 1979 | 531719 |
| immunochemical detection of n6-methyladenine in dna. | antibodies to n6-methyladenosine were produced in rabbit by means of immunization with n6-methyladenosine coupled to bovine serum albumin via periodate oxidation. cross-reacting antibodies were removed by bovine serum albumin-sepharose and appropriate nucleoside-human serum albumin absorbents. nucleoside-specific antibodies were isolated by affinity chromatography of n6-methyladenosine-human serum albumin-sepharose. the specificity of the purified antibodies has been demonstrated by complement f ... | 1979 | 534639 |
| serological grouping of streptococci by slide agglutination. | streptococcal grouping sera for groups a, b, c, and g prepared for conventional testing by precipitation were made specific by absorption and used to identify streptococci by slide agglutination with and without staphyloccocal coagglutination. trypsinised suspensions of 1055 strains, identified by precipitation as belonging to group a, b, c, or g, were tested by slide agglutination. of these, 998 were correctly identified using a streptococcal suspension and antisera alone and a further 65 were ... | 1979 | 536459 |
| hydroheptin: a water-soluble polyene macrolide. i. taxonomy, fermentation and isolation. | a soil isolate of streptomyces, which has been deposited in the culture collection of the waksman institute of microbiology, rutgers university as imru 3962, produces a new heptaene macrolide antifungal antibiotic, hydroheptin. the producing microorganism, which co-produces the antibiotic, chartreusin, has been identified as a strain of streptomyces chartreusis. fermentation and bioassay procedures were developed for the production and analysis of hydroheptin and chartreusin. isolation and purif ... | 1979 | 541249 |
| hydroheptin: a water-soluble polyene macrolide. ii. chemical and biological properties. | hydroheptin, a new polyene macrolide antifungal antibiotic, is co-produced with the antibiotic, chartreusin, by a strain of streptomyces chartreusis designated as imru 3962 isolated in our laboratory. the unique water-solubility of this antibiotic at neutrality, revealing in aqueous solution molecular dispersion and an ultraviolet-visible absorption spectrum characteristic of an all-trans heptaene chromophore, clearly distinguishes it from all previously-described and naturally-occurring heptaen ... | 1979 | 541250 |
| staphcoccomycin, a new basic macrolide antibiotic. | staphcoccomycin (scm) is a new member of the basic macrolide family of antibiotics which was isolated from the fermentation broth of streptomyces sp. as-ng 16. the production, purification and determination of physical and chemical properties of this novel metabolite have been completed. comparison of the mass fragmentation patterns of scm and its peracetate with those of angolamycin peracetate suggested a des-mycarosyl derivative of angolamycin. moreover, the molecular ion peak (m/e 771) corres ... | 1979 | 541251 |
| pamamycin: a new antibiotic and stimulator of aerial mycelia formation. | pamamycin is a new antibiotic isolated from streptomyces alboniger atcc 12461. the antibiotic is active in vitro against gram-positive bacteria, neurospora, and mycobacteria. the compound also acts as a streptomycete differentiation effector. it stimulates aerial mycelia formation in the producing organism. the new antibiotic of elemental composition c36h63no7 is completely different from puromycin, also produced by this strain. the present communication deals with the isolation, properties, and ... | 1979 | 541261 |
| the enzymatic interconversion between midecamycin a1 and a3. | 1979 | 541276 | |
| characterization and persistence of actinophage rp2 isolated from streptomyces rimosus atcc 10970. | while searching for true lysogens among oxytetracycline-producing streptomyces rimosus strains, free phage particles were detected and isolated from a liquid culture of s. rimosus atcc 10970 (r7). the actinophage, designated rp2, appears to be a typical temperate dna phage producing turbid plaques on the sensitive strain s. rimosus r6. electron microscopic examination of rp2 lysates showed that it belongs to group b of bradley's morphological classification. the rate of rp2 adsoprption at 28 deg ... | 1979 | 541659 |
| solubilization of membrane-bound transpeptidase from streptomyces strain k-11 with 2,3-dimethylmaleic anhydride. | 1979 | 542711 | |
| isolation of a protoplast-type l-form from streptomyces hygroscopicus. | 1979 | 543157 | |
| [metabolic products of microorganisms 183, imacidin, a new peptid antibiotic from streptomyces olivaceus (author's transl)]. | streptomyces olivaceus, strain tü 1379 produces beside the red dyestuff prodigiosin several closely related hitherto unknown cyclodepsipeptide antibiotics, mainly imacidine b and c and imacidinic acids. the antibiotics affect murein biosynthesis. only actinomycetes are inhibited. | 1979 | 543774 |
| distribution functions of variables characterizing the mycelial morphology of streptomyces hygroscopicus grown in glucose-limited chemostat cultures. | the distribution of variables characterizing the morphology of the mycelium of streptomyces hygroscopicus grown in glucose-limited chemostat cultures at different specific growth rates were investigated statistically. the values of the hyphal growth unit (l/n) and the values of the distance from the apex to the first branch (lp) are normally distributed, but the values of the distance between neighbouring branches are logarithmically normal distributed. the distribution functions are discussed f ... | 1979 | 545910 |
| growth kinetics and antibiotic synthesis during the repeated fed-batch culture of streptomycetes. | 1979 | 548134 | |
| notes on the genus chainia. | 1979 | 548522 | |
| a new variety of chainia antibiotica showing antisarcina activity. | 1979 | 548525 | |
| fermentative production of cobalamin by streptomyces olivaceus. | 1977 | 548700 | |
| production of the antibiotic nrc-501 isolated from fermentation cultures of streptomyces species, using egyptian molasses. | 1977 | 548701 | |
| stereochemical structure recognized by the l-fucose-specific hemagglutinin produced by streptomyces sp. | a hemagglutinin has been purified 4000-fold from the culture filtrate of a strain of streptomyces by affinity chromatography. the purified preparation was judged to be homogeneous by gel electrophoresis and its molecular weight was estimated to be about 70 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. it may exhibit its full hemagglutinating activity in the monomer form. this hemagglutinin strongly agglutinated human blood group o erythrocytes and was inhibited by l-fucose. i ... | 1979 | 549650 |
| aminoglycosides, genes and regulation. | a major role has been played by aminoglycoside research in the development of fermentation science especially involving genetics and regulation. the first report of the use of an antibiotic to select high producers of that antibiotic was done with the streptomycin producer. mutational biosynthesis of new antibiotics originated with studies on the neomycin producer. the first demonstration of the presence of genes coding for antibiotic inactivation in antibiotic producers was made with streptomyc ... | 1979 | 549988 |
| glycosaminoglycan synthesis by human chondrosarcoma. | human chondrosarcoma of low-grade malignancy was cultured in the presence of 35s-sulphate and 3h-glucosamine. the glycosaminoglycans isolated were fractionated on ecteola cellulose and electrophoresed on cellulose acetate membranes before and after treatment with chondroitinase ac or streptomyces hyaluronidase. the results demonstrated the in vitro synthesis of hyaluronate, chondroitin sulphate and keratan sulphate. the presence of keratan sulphate of large average chain length (congruent to 15 ... | 1979 | 550432 |
| a xanthine-negative, melibiose-positive species of streptomyces. | the organism vh/cn-71 which had been isolated from a growth in a cat and described initially as an "unusual strain of nocardia" is a streptomycete. this organism can also produce acid from melibiose although it is unable to decompose xanthine. its sporophores are short, hooked, curved, or open-looped with extended spirals. present data suggest that vh/cn-71 may belong to the "flavus" series of streptomyces. | 1977 | 556974 |
| streptomyces novoguineensis sp. nov., an amipurimycin producer, and antimicrobial activity of amipurimycin. | a taxonomic study of streptomyces strain t-36496, which produces an antibiotic effective against rice blast, revealed that it represented a new taxon and it was named streptomyces novoguineensis sp. nov. the antibiotic, which was named amipurimycin, showed antifungal activity in vitro and considerable curative effect onleaf blast both in green house and field tests at concentrations ranging from 10 to 20 ppm. it was also effective against neck and panicle blast at the same concentration range. | 1977 | 557031 |
| mycetomas in madras. | in 90 of 150 cases clinically diagnosed as mycetoma in the department of pathology, madras medical college, from january 1964 to june 1975, histopathological study revealed granules. besides special staining procedures, cultural methods were undertaken in recent cases. the age and sex incidence, site of lesion and the species of fungi identified on the basis of histological morphology of the granules are analysed. the disease was predominatly seen in the age group 21-40. men were more frequently ... | 1977 | 558655 |
| interactions of hg(ii) ions with dna as revealed by cd measurements. | the circular dichroism spectra of hg(ii) complexes with native calf thymus dna, chemically methylated streptomyces chrysomallus dna and with ag(i)-dna complexes were measured in the region of 220 - 340 nm. as a main result a conversion of the conservative cd spectrum of dna to a distinct nonconservative type of cd spectrum for the complexes occurs with increasing hg(ii) concentration. the cd spectra of the hg(ii) complexes as well as some additional arguments strongly support the idea, that dna ... | 1977 | 559300 |
| baumycins, new antitumor antibiotics related to daunomycin. | 1977 | 561047 | |
| antibiotics produced by streptomyces olivaceus 142. iii. influence of antibiotic wr 142 fpg on development of transplantable tumors in mice. | the action of two preparations (i and ii) of antibiotic wr 142 fpg on development of ehrlich carcinoma, nemeth-kellner lymphoma and leukemias l 1210 and p 388 was studied. preparation i injected s.c. daily during 14 days in doses of 10 mg/kg (1/25 ld50) inhibited growth of ehrlich carcinoma and nemeth-kellner lymphoma subcutaneous tumors in r 3 mice by about 70%. the same preparation in a single i.p. injection (10 mg/kg) in fdf1 mice distinctly prolonged survival of mice inoculated with l 1210 l ... | 1977 | 565628 |
| esterastin, an inhibitor of esterase, produced by actinomycetes. | 1978 | 567214 | |
| immunological detection of 20beta-hydroxysteroid dehydrogenase-synthesizing polysomes from streptomyces hydrogenans. | immunoprecipitation of polyribosomes from streptomyces hydrogenans synthesizing 20beta-hydroxysteroid dehydrogenase was performed by immunoelectrophoresis. only polyribosomes from cells induced by 11beta, 21-dihydroxy-4, 11(20)-pregnadien-3-one in vivo were precipitated by immunoglobulins specific for 20beta-hydroxysteroid dehydrogenase. no unspecific precipitations with polysomes from control cells cultivated in the absence of steroids were observed. therefore, 11beta, 21-dihydroxy-4, 17(20)-pr ... | 1978 | 569951 |
| degradation of organic nitrogenous wastes by a soil streptomycete. | a soil streptomycete degraded hair, silk, wool, feather and leather which were collected from solid wastes. the organism was identified taxonomically and designated streptomyces sp. a956. it degraded leather to the maximum extent and solubilized 35.9% of the total nitrogen, 2.32 mg of glycine equivalent amino nitrogen could be obtained by degradation of 100 mg leather. | 1979 | 574480 |
| [new antibiotic no. 792 formed by actinomyces bottropensis]. | an actinomycetes strain 792 producing a new antibiotic was isolated under the programme of antitumor antibiotic screening. by its morphological and cultural properties strain 792 was classified as belonging to species actinomyces bottropensis. antibiotic 792 was recovered from the culture fluid of the strain by the extraction method in the form of a crystalline orange substances. lambda max 235, 305, 410 nm (e 1% 1cm 705, 105, 168), m. p. 232-255 degrees (dec), molecular weight 340, c 67 per cen ... | 1977 | 576572 |
| antitumor activity of new anthracycline antibiotics, aclacinomycin-a and its analogs, and their toxicity. | new anthracycline antibiotics have been isolated from the culture of streptomyces galilaeus ma144-m1. among 14 anthracycline compounds, aclacinomycin-a showed the strongest activity in inhibiting leukemia l-1210 and had lower toxicity than others. antitumor activity of aclacinomycin-a against leukemia l-1210 and p-388, solid sarcoma-180, and lymphosarcoma 6c3hed was examined in comparison with adriamycin and daunomycin. aclacinomycin-a showed the same degree of activity against leukemia l-1210 a ... | 1977 | 579346 |
| [effect of organic acids on the biosynthesis of macrotetralide antibiotics by an actinomyces chrysomallus var. carotenoides strain]. | the biosynthesis of macrotetrolides by actinomyces chrysomalus var. carotenoides was stimulated by acetic, succinic, propionic, oxalic, malic, tartaric, citric, pyruvic, alpha-ketoglutaric and fumaric acids. incorporation of 14c-acetate into the molecule of the antibiotic and the data on dependence of the stimulating effect upon the quantitative ratio and time of the organic acid addition were indicative of the role of acetic, succinic and propionic acids as precursors of macrotetrolides. the ot ... | 1978 | 581049 |
| pyrrolo (1,4) benzodiazepine antitumor antibiotics: biosynthetic studies on the conversion of tryptophan to the anthranilic acid moieties of sibiromycin and tomaymycin. | biosynthetic intermediates between tryptophan and the anthranilate moieties of tomaymycin and sibiromycin have been suggested, based upon a combination of feeding experiments with either carbon-14-labeled substrates or competition experiments between radiolabeled tryptophan and unlabeled intermediates. in the case of sibiromycin and tomaymycin, substitution of the aromatic ring most likely takes place at the kynurenine stage. feeding experiments with the anthramycin culture were inconclusive, mo ... | 1979 | 581831 |
| biosynthesis of chloramphenicol in streptomyces species 3022a: the nature of the arylamine synthetase system. | the arylamine synthetase which catalyses the conversion of chorismic acid to p-aminophenylalanine in streptomyces species 3022a was separated from aminotransferase by deae-cellulose chromatography. recovered activity was further fragmented into three separate components by passage through a sephadex g-100 column. only one component produced p-aminophenylalanine but in combination the three stimulated each other activity. although the products of the other two components were unstable, an infrare ... | 1979 | 583399 |
| selection of a chemically defined medium for submerged cultivation of streptomyces aureofaciens with high extracellular caseinolytic activity. | a chemically defined medium was developed for the submerged cultivation of streptomyces aureofaciens with a high secretion of caseinolytic activity. the medium composition is: 40 g/liter maltose; 1.640 g/liter l-leucine (0.0125m); 1.765 g/liter l-lysine (0.0125m); 6.976 g/liter k2hpo4 (0.04m); 4 g/liter caco3; 0.2 g/liter mgso4.7h2o; 0.01 g/liter znso4.7h2o; 0.01 g/liter feso4.7h2o: 0.01 g/liter mnso4h2o, and 0.005 g/liter coso4.7h2o. quantitative correlations were established between the concen ... | 1977 | 588671 |
| the peptidoglycan crosslinking enzyme system in streptomyces strains r61, k15 and rimosus. | the dd-carboxypeptidase-transpeptidase enzyme system in streptomyces strain k15 consists of: (1) a membrane-bound transpeptidase capable of performing low dd-carboxypeptidase activity; and (2) a set of dd-carboxypeptidases: (a) membrane-bound, (b) lysozyme-releasable and (c) exocellular, having low transpeptidase activities in aqueous media and at low acceptor concentrations. the dd-carboxypeptidases are related to each other and may belong to the same pathway leading to enzyme excretion. a simi ... | 1977 | 590266 |
| the peptidoglycan crosslinking enzyme system in streptomyces r61, k15 and rimosus. immunological studies. | 1977 | 590268 | |
| the peptidoglycan crosslinking enzyme system in streptomyces strains r61, k15 and rimosus. kinetic coefficients involved in the interactions of the membrane-bound transpeptidase with peptide substrates and beta-lactam antibiotics. | the transpeptidation reaction performed by the membranes of streptomyces strain r61 fits the general rate equation for an enzyme-catalysed bimolecular reaction. the same membranes (e) interact with beta-lactams (i) to form inactive penicillin-enzyme-membrane complexes (ei) of rather high stability, which subsequently break down (e + i leads to ei leads to e + degradation products). the enzyme is regenerated and the antibiotic is released in the form of an inactive metabolite. with benzylpenicill ... | 1977 | 590269 |
| preparative method for the isolation of super-coiled dna from a chloramphenicol-producing streptomycete. | 1977 | 591452 | |
| new antibiotics saframycins a, b, c, d and e. | 1977 | 591455 | |
| studies on the biosynthesis of blasticidin s. vi. the isolation and structure of blasticidin h. | 1977 | 591456 | |
| studies on the biosynthesis of blasticidin s. vii. isolation of demethylblasticidin s. | 1977 | 591457 | |
| mutalomycin, a new polyether antibiotic taxonomy, fermentation, isolation and characterization. | mutalomycin is a new metal-complexing polyether antibiotic produced by a strain of streptomyces mutabilis nrrl 8088. the metabolite, a monocarboxylic acid, was isolated as the sodium salt c41h69nao12. the structure of this polyether was established by x-ray analysis of its potassium salt c41h69ko12. mutalomycin contains six heterocyclic rings and is structurally related to nigericin. the metabolite is active against gram-positive bacteria and eimeria tenella (chicken coccidiosis). | 1977 | 591458 |
| new antitumor antibiotics, os-4742 a1, a2, b1 and b2 produced by a strain of streptomyces. | new antibiotics, os-4742 a1, a2, b1 and b2, were isolated from the culture broth of the strain os-4742, which was designated as streptomyces matensis subsp. vineus. these compounds have anthracycline chromophores and sugar moieties, but do not contain nitrogen. they possess antimicrobial activities against gram-positive bacteria and antitumor activities against s-180 solid tumor on mice. | 1977 | 591459 |
| the states of tyrosyl and tryptophyl residues in a protein proteinase inhibitor (streptomyces subtilisin inhibitor. | the states of tyrosyl and tryptophyl residues of a dimeric protein proteinase inhibitor, streptomyces subtilisin inhibitor (sato, s & murao, s. (1973), agric. biol. chem. 37, 1067) were studies by solvent perturbation difference spectroscopy with methanol, ethylene glycol, polyethylene glycol, and deuterium oxide as perturbants, and by spectrophotometric titration at alkaline ph. it appeared that all three tyrosyl residues per monomer of the inhibitor were exposed on the surface of the molecule, ... | 1977 | 591497 |
| genetic recombination through protoplast fusion in streptomyces. | 1977 | 593313 | |
| [formation of new antibiotic, virenomycin, by a culture of streptomyces virens sp. nev]. | a culture of a new species streptomyces virens was isolated from a soil sample. it produced an antibiotic designated as virenomycin. the antibiotic was mainly synthesized in the mycelium. only insignificant amounts of it were found in the culture fluid. the optimal nutrient medium for production of virenomycin contained glycerol, soybean meal, ammonium sulphate, sodium chloride and calcium carbonate. crystalline virenomycin had a comparatively low antitumor activity and narrow spectrum. | 1977 | 596841 |