Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| ultrastructure of the surface film of bacterial colonies. | the structure of the surface of colonies of various gram-negative and gram-positive bacteria was examined by transmission electron microscopy. the results indicate that bacterial colonies in the course of their development produce a film which becomes thicker with increased duration of growth. the basic part of the film is an elementary membrane, which is a stable structure with a large surface area. the inner and outer surfaces of the film membrane are covered by amorphous layers. these layers ... | 1993 | 8515240 |
| chemotaxonomy and molecular taxonomy of some coryneform clinical isolates. | six reference strains of the genus brevibacterium as well as fifteen clinical isolates tentatively assigned to the genus using conventional biochemical methods, were the subject of chemotaxonomic and dna similarity studies. five of these clinical isolates were assigned either to the genera aureobacterium, mycobacterium, gordona or to rhodococcus on the basis of their dna mol% g+c, mycolic acid, amino-acid, sugar and menaquinone contents. among the ten remaining strains, six were not brevibacteri ... | 1993 | 8518510 |
| prophage distribution in coryneform bacteria. | four temperate bacteriophages of corynebacteria were isolated after uv induction. phages phi 304l and phi 304s were both induced from corynebacterium glutamicum atcc 13058, atcc 21488, atcc 21649 and atcc 21650 strains, and have no known sensitive host. phages phi 15 and phi 16 were both induced from atcc 14020 and atcc 21792. phage phi 15 formed turbid plaques on corynebacterium sp. atcc 21857 and on c. glutamicum atcc 13058, atcc 21488, atcc 21649 and atcc 21650. phage phi 16 produced turbid p ... | 1995 | 8525066 |
| bacteremia caused by brevibacterium species in an immunocompromised patient. | a 46-year-old woman suffering from non-hodgkin's lymphoma was admitted to the hospital because of high fever. multiple blood cultures revealed an unusual finding, a brevibacterium species, which was reisolated 16 days later from the tip of her long-term central venous catheter. this case indicates that brevibacterium species isolated from normally sterile sites should be considered as a potential pathogen, especially in immunocompromised patients. | 1995 | 8536730 |
| sizing of the rhodococcus sp. r312 genome by pulsed-field gel electrophoresis. localization of genes involved in nitrile degradation. | the two restriction enzymes asni and drai were found to produce dna fragment sizes that could be used for mapping the rhodococcus sp. r312 (formerly brevibacterium sp. r312) genome by pulsed-field gel electrophoresis. asni produced 24 fragments (4 to 727 kb) and drai yielded 15 fragments (8.5 to 2400 kb). the fragment lengths in each digest were summed, indicating that the size of the chromosome ranged from 6.31 to 6.56 mb, with a mean of 6.44 mb. in addition, the wide-spectrum amidase gene (ami ... | 1995 | 8546455 |
| multiple sigma factor genes in brevibacterium lactofermentum: characterization of siga and sigb. | four rpod hybridizing signals have been identified in the chromosome of brevibacterium lactofermentum. two rpod-like genes, siga and sigb, have been cloned and sequenced, and they encode principal sigma factors of the rna polymerase. the deduced amino acid sequences of siga and sigb showed very high similarities to those of mycobacterium smegmatis mysa and mysb proteins, respectively, and also to those of hrdb proteins from different streptomyces species. siga and sigb maintain the conserved mot ... | 1996 | 8550480 |
| the influence of the ph-value on the growth of brevibacterium epidermidis in continuous culture. | brevibacterium epidermidis is a major component of the bacterial flora of certain skin surface biotopes, characterized by a comparatively high ph-value. the presence of brevibacterium epidermidis seems to be linked to the production of malodour. skin surface ph has been found to be a major factor of bacterial growth on the skin. in order to find out if this might also apply to brevibacterium epidermidis, this microorganism was grown in vitro in continuous culture using a chemostat. specific grow ... | 1995 | 8578948 |
| [heterogeneity of peptidoglycans of bacterial origin]. | heterogeneity of peptidoglycans of staphylococcus aureus of strains 3272 and wood-46, brevibacterium flavum, micrococcus lysodeikticus has been studied by the method of electrophoresis in the system polyacrylamide gel--sodium dodecylsulfate. it is shown that peptidoglycans of different strains of s. aureus and other species of gram-positive bacteria treated with ultrasound are a complex of fractions with 3 basic high-molecular components with m 100, 92 and 84 kda, while two main fractions with m ... | 1995 | 8588262 |
| specificity of an extracellular proteinase from brevibacterium linens atcc 9174 on bovine alpha s1-casein. | the specificity of the extracellular proteinase from brevibacterium linens atcc 9174 on bovine alpha s1-casein was studied. hydrolysis was monitored over time by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) and urea-page. the major ph 4.6-soluble peptides were isolated by high-performance liquid chromatography and identified by n-terminal amino acid sequencing and mass spectrometry. the time course of peptide formation indicated that his-8-gln-9, ser-161-gly-162, and either g ... | 1996 | 8593051 |
| genetic characterization of site-specific integration functions of phi aau2 infecting "arthrobacter aureus" c70. | all the essential genetic determinants for site-specific integration of corynephage phi aau2 are contained within a 1,756-bp dna fragment, carried on the integrative plasmid p5510, and are shown to be functional in escherichia coli. one open reading frame, orf4, encoding a protein of 266 amino acids was shown to represent the phi aau2 integrase. the nucleotide sequence of the phi aau2 attachment site, attp, and the attb, attl, and attr sequences in the host "arthrobacter aureus" c70 were determi ... | 1996 | 8606175 |
| cloning and characterization of an is-like element present in the genome of brevibacterium lactofermentum atcc 13869. | a repetitive dna element of the gram+ brevibacterium lactofermentum (bl), cloned by a modification of the subtractive hybridization method, contained a 1.4-kb is-like element, is13869, which included an open reading frame (orf) inside a perfect 26-bp terminal inverted repeat (tir). an 8-bp direct repeat (dr) was found outside each tir. the orf encoded a deduced protein of 436 amino acids (49 380 da) with extensive similarity to other known transposases of insertion elements of mycobacterium smeg ... | 1996 | 8621097 |
| homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum. a dioxygenase with catalase activity. | homoprotocatechuate 2,3-dioxygenase (2,3-hpcd) cleaves the aromatic ring of its substrate with insertion of both atoms of oxygen from o2 to form alpha-hydroxy- delta-carboxymethyl cis-muconic semialdehyde. the enzyme has been purified from the gram-positive bacterium brevibacterium fuscum and characterized. the enzyme appears to have a range of quaternary structures with predominant components of alpha4 and alpha6 (alpha subunit mr = 42500 +/- 1500) and binds approximately 1 fe(ii)/subunit. alth ... | 1996 | 8621411 |
| the purification and characterisation of flavin adenine dinucleotide phosphohydrolase from brevibacterium ammoniagenes. | 1996 | 8674672 | |
| recurrent bacteremia due to brevibacterium casei in an immunocompromised patient. | a case of an immunocompromised patient who experienced two episodes of septicemia caused by a coryneform bacterium is reported. biochemical characteristics and analysis of cellular fatty acids and of cell wall components showed two identical strains of brevibacterium casei to be responsible for these infections. the lack of easy-to-perform methods for identification may have led, in the past, to an underestimation of the role of this bacterium, especially in immunocompromised patients. | 1995 | 8681984 |
| cloning and expression of the bali restriction-modification system. | bali, a type ii restriction-modification (r-m) system from the bacterium, brevibacterium albidum, recognizes the dna sequence 5'-tggcca-3'. we cloned the genes encoding the bali restriction endonuclease and methyltransferase and expressed them in escherichia coli. the two genes were aligned tail-to-tail and their termination codons overlapped. bali restriction endonuclease and methyltransferase comprise 260 and 280 amino acids, respectively, and have molecular weights of 29 043 and 31 999 da. th ... | 1996 | 8710495 |
| [the serological properties of a lysine producer developing in a batch culture]. | immune sera specific to bacterial cells at various development stages of periodic culture have been obtained with the purpose to study serological properties of lysin producer. using the method of immunoenzyme analysis elisa it is shown that there is certain dependence between the producer properties and the culture age. different strains-producers used in production to-day are serologically close between each other. when using 49 strains-contaminants of lysin production (representatives of 10 g ... | 1996 | 8729466 |
| resonance raman spectroscopy of nitrile hydratase, a novel iron-sulfur enzyme. | resonance raman spectra of rhodococcus sp. r312 (formerly brevibacterium sp. r312) nitrile hydratase, a novel non-heme iron enzyme, have a large number of peaks in the 300-500 cm-1 region; observation of shifts in these peaks after labeling with 34s shows that they arise from cysteine coordinated to the ferric ion in the protein. the rich raman spectra result from coupling of the fe-s stretch with cysteine side chain deformation modes; the observation of 15n isotope shifts in most of these peaks ... | 1996 | 8756469 |
| x-ray spectroscopy of nitrile hydratase at ph 7 and 9. | the iron k-edge x-ray absorption spectrum of rhodococcus sp. r312 (formerly brevibacterium sp. r312) nitrile hydratase in frozen solutions at ph 7 and 9 has been analyzed to determine details of the iron coordination. exafs analysis implies two or three sulfur ligands per iron and overall six coordination; together with previous epr and endor results, this implies an n3s2o ligation sphere. the bond lengths from exafs analysis [rav(fe-s) = 2.21 a at ph 7.3; rav(fe-n/o) = 1.99 a] support cis coord ... | 1996 | 8756470 |
| identification and functional differentiation of two type i fatty acid synthases in brevibacterium ammoniagenes. | the fatty acid synthase (fas) from brevibacterium ammoniagenes is a homohexameric multienzyme complex that catalyzes the synthesis of both saturated and unsaturated fatty acids. by immunological screening of a b. ammoniagenes expression library, an fas dna fragment was isolated and subsequently used to clone the entire gene together with its flanking sequences. within 10,525 bp of sequenced dna, the 9,189-bp fas coding region was identified, corresponding to a protein of 3,063 amino acids with a ... | 1996 | 8759839 |
| molecular cloning of the hom-thrc-thrb cluster from bacillus sp. ulm1: expression of the thrc gene in escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes. | a 6.5 kb dna fragment containing the gene (thrc) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the dna of bacillus sp. ulm1 by complementation of escherichia coli and brevibacterium lactofermentum thrc auxotrophs. complementation studies showed that the thrb gene (encoding homoserine kinase) is found downstream from the thrc gene, and analysis of nucleotide sequences indicated that the hom gene (encoding homoserine dehydrogenase) is loca ... | 1995 | 8768250 |
| key role of alkanoic acids on the spectral properties, activity, and active-site stability of iron-containing nitrile hydratase from brevibacterium r312. | interaction of n-butyric acid with dialyzed nitrile hydratase from brevibacterium r312, which is characterized by a charge-transfer band at 680 nm and epr signals typical of a low-spin fe(iii) with delta g = 0.22, leads to a form displaying different spectral properties (lambda = 710 nm, delta g = 0.31). butyric acid also acts as a competitive inhibitor of nitrile-hydratase-catalyzed hydration of acrylonitrile with a ki value of 0.9 mm. formation of the complex between the enzyme and butyric aci ... | 1996 | 8797859 |
| crystallization and preliminary x-ray analysis of cholesterol oxidase from brevibacterium sterolicum containing covalently bound fad. | single crystals of cholesterol oxidase from brevibacterium sterolicum containing a covalently bound form of the fad cofactor have been obtained. the crystals are grown by vapor diffusion using the hanging drop technique from 12% polyethylene glycol, mr 8000, and 75 mm mnso4 as the precipitant at ph 5.2. in order to obtain large diffraction quality crystals, nucleation must occur at 22 degrees c with subsequent growth at 17 degrees c. the crystals belong to the monoclinic space group p21 with cel ... | 1996 | 8812988 |
| integration of narrow-host-range vectors from escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. | conjugative transfer of mobilizable derivatives of the escherichia coli narrow-host-range plasmids pbr322, pbr325, pacyc177, and pacyc184 from e. coli to species of the gram-positive genera corynebacterium and brevibacterium resulted in the integration of the plasmids into the genomes of the recipient bacteria. transconjugants appeared at low frequencies and reproducibly with a delay of 2 to 3 days compared with matings with replicative vectors. southern analysis of corynebacterial transconjugan ... | 1996 | 8824624 |
| an identification scheme for rapidly and aerobically growing gram-positive rods. | an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ... | 1996 | 8837385 |
| molecular control mechanisms of lysine and threonine biosynthesis in amino acid-producing corynebacteria: redirecting carbon flow. | threonine and lysine are two of the economically most important essential amino acids. they are produced industrially by species of the genera corynebacterium and brevibacterium. the branched biosynthetic pathway of these amino acids in corynebacteria is unusual in gene organization and in the control of key enzymatic steps with respect to other microorganisms. this article reviews the molecular control mechanisms of the biosynthetic pathways leading to threonine and lysine in corynebacteria, an ... | 1996 | 8837462 |
| analysis of nucleotide methylation in dna from corynebacterium glutamicum and related species. | plasmid dna (pcsl17) isolated from corynebacterium glutamicum transformed recipient mcrbc+ strains of escherichia coli with lower efficiency than mcrbc- strains, confirming a previous report by tauch et al. (fems microbiol. lett. 123 (1994) 343-348) which inferred that c. glutamicum dna contains methylcytidine. analysis of nucleotides in c. glutamicum-derived chromosomal and plasmid dna failed to detect significant levels of methylated adenosine, but methylated cytidine was readily detected. res ... | 1996 | 8867385 |
| cloning and sequencing of the secy homolog from streptomyces lividans 1326. | two conserved regions of secy proteins from six gram+ bacteria were exploited in a pcr-based strategy for isolating a secy homolog from streptomyces lividans (sl). the nucleotide sequence of part of a 3.8-kb fragment showed that the secy homolog is flanked, at the 5' end, by the gene encoding ribosomal protein l15 and, at the 3' end, by an adenylate kinase-encoding gene. the deduced gene product of secy would have 437 amino acids (aa) and an m(r) of 47,200. sl secy shows 89.5, 56.1, 42 and 40% i ... | 1996 | 8918265 |
| nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from brevibacterium linens m18. | linocin m18 is an antilisterial bacteriocin produced by the red smear cheese bacterium brevibacterium linens m18. oligonucleotide probes based on the n-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal dna. the amino acid composition, n-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb bamhi restriction fragment correspond closely to those o ... | 1996 | 8919789 |
| the gale gene encoding the udp-galactose 4-epimerase of brevibacterium lactofermentum is coupled transcriptionally to the dmdr gene. | the gale gene of brevibacterium lactofermentum, encoding udp-galactose 4-epimerase (ec 5.1.3.2), has been identified by dna sequencing downstream from the orf1-sigb-dmdr region. the arrangement of the sigb-dtxr-gale cluster is also conserved in corynebacterium diphtheriae. the deduced gale product was a protein of 329 aa residues (35.4 kda) that shared a high degree of identity to known udp-galactose 4-epimerase proteins from gram-positive microorganisms (streptomyces lividans and streptococcus ... | 1996 | 8921853 |
| construction of new cloning vectors for brevibacterium lactofermentum. | two plasmid cloning vectors (pulmj55 and pulmj95) were constructed for brevibacterium lactofermentum using the origin of replication of the endogenous plasmid pbl1. plasmid pulmj55 is a replacement vector with transcriptional terminators from the b. lactofermentum trp operon flanking the bglii cloning sites. religation of the bglii digested vector without insert creates a 376 bp perfect palindrome that is not tolerated in b. lactofermentum, giving positive selection for recombinant plasmids with ... | 1996 | 8935658 |
| a brevibacterium linens prbl1 replicon functional in corynebacterium glutamicum. | brevibacterium linens rbl strain cryptic plasmid prbl1 (8.0 kb) is described. a region involved in prbl1 autonomous replication in corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. this region encodes for a hypothetical 310-amino acid (aa) protein closely related to the replicases of plasmids pxz10142 (c. glutamicum) and pal5000 (mycobacterium fortuitum). the 310-aa protein also shows significant homology to proteins of pcole5-099 (shigella sonn ... | 1996 | 8938050 |
| a cryptic plasmid pbl1 from brevibacterium lactofermentum causes growth inhibition and filamentation in escherichia coli. | a shuttle vector composed of pbl1, a 4.46-kb cryptic plasmid from coryneform bacterium brevibacterium lactofermentum, and escherichia coli vector phsg298 was found to inhibit growth and cause cell filamentation in e coli. after construction of several deletions of pbl1, a 1.23-kb acci-hindiii fragment was found responsible. dna sequence analysis showed that this fragment contained a 726-bp-long open reading frame (orf3), encoding a protein with 242 amino acid residues. corresponding to orf3, a 2 ... | 1996 | 8938054 |
| cloning of the tryptophan operon of brevibacterium divaricatum and its expression in e. coli. | a genomic bank from brevibacterium divaricatum has been prepared using lambda embl3 as a vector. the genomic bank's titers are 2.2 x 10(6) pfu/micrograms. through screening by plaque hybridization, a 9.6 kb ncoi fragment which contains the entire trp operon has been isolated. polymerase chain reaction amplification and restriction endonuclease analysis of pcr fragments indicated that there is homology between the coryneform bacteria; however, some genetic diversity among the species still exists ... | 1996 | 8956524 |
| molecular cloning of a novel gene, dtsr, which rescues the detergent sensitivity of a mutant derived from brevibacterium lactofermentum. | several strains of corynebacterium and brevibacterium are known for their ability to secrete large amounts of amino acids, especially l-glutamate. we focused on the mechanism of l-glutamate secretion triggered by a detergent, namely polyoxyethylenesorbitan monopalmitate (pesp). a mutant strain, aj11060, derived from brevibacterium lactofermentum atcc 13869 indicates the sensitivity to pesp. a multicopy suppresser gene that compliments the sensitivity of aj11060 to the detergent was derived from ... | 1996 | 8987652 |
| clinical microbiology of coryneform bacteria. | coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ... | 1997 | 8993861 |
| genomic organization of purk and pure in brevibacterium ammoniagenes atcc 6872: pure locus provides a clue for genomic evolution. | from the genomic library of brevibacterium ammoniagenes atcc6872, the pure locus encoding 5'-phosphoribosyl-5-aminoimidazole (air) carboxylase (ec 4.1.1.21) was cloned and its nucleotide sequence was determined. from the sequence analysis, two distinct open reading frames (orfs) in the sequence of the pure locus were identified as purk and pure genes (purk-pure). an in vivo translation experiment reconfirmed the purk and pure genes to be independent. the genomic organization in the pure locus of ... | 1996 | 8998996 |
| electron spin echo envelope modulation studies of the semiquinone anion radical of cholesterol oxidase from brevibacterium sterolicum. | the electron spin echo envelope modulation (eseem) technique of pulsed epr spectroscopy was applied to the anionic semiquinone of the cholesterol oxidase flavin cofactor, formed when the enzyme was photoreduced in the presence of 5-deazariboflavin and edta. fourier transforms of the three-pulse eseem spectra showed the presence of 14n nuclei magnetically coupled to the paramagnet. in 2h2o buffer the surroundings of the flavin ring were shown to be accessible to solvent exchange, with a deuterium ... | 1997 | 9001407 |
| molecular cloning of the corynebacterium glutamicum ('brevibacterium lactofermentum' aj12036) odha gene encoding a novel type of 2-oxoglutarate dehydrogenase. | the corynebacterium glutamicum ('brevibacterium lactofermentum' aj12036) odha gene, encoding 2-oxoglutarate dehydrogenase (e1o subunit of the 2-oxoglutarate dehydrogenase complex), has been isolated and identified as an homologous counterpart of the escherichia coll suca and bacillus subtilis odha genes. the nucleotide sequence of a 4394 bp chromosomal fragment containing the c. glutamicum odha gene was determined. the odha gene comprised 3771 bp (1257 codons, including the initiation codon) and ... | 1996 | 9004499 |
| construction of l-lysine-overproducing strains of brevibacterium lactofermentum by targeted disruption of the hom and thrb genes. | the mobilization of plasmids from gram-negative escherichia coli to gram-positive brevibacterium lactofermentum, mediated by p-type transfer functions, was used to construct disrupted mutants blocked specifically in the homoserine branch of the aspartate pathway. the mutant strain b. lactofermentum r31 showed an efficiency of conjugal transfer two to three orders of magnitude higher than that of the wild-type strain b. lactofermentum atcc 13869. the hom- and thrb-disrupted mutants of b. lactofer ... | 1996 | 9008889 |
| effects of an escherichia coli ilva mutant gene encoding feedback-resistant threonine deaminase on l-isoleucine production by brevibacterium flavum. | a mutated ilva gene of escherichia coli encoding a threonine deaminase that is resistant to feedback inhibition by l-isoleucine was obtained. it was functional in brevibacterium flavum, and a wild strain of b. flavum into which it was introduced became able to convert exogeneous l-homoserine and l-threonine to l-isoleucine. when it was introduced into a l-threonine-producing b. flavum strain, the transformant accumulated 20 g/liter l-isoleucine from 100 g/liter glucose. | 1997 | 9028039 |
| identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. | coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ... | 1997 | 9039559 |
| reclassification of "bacillus pulvifaciens" group ii as brevibacillus agri. | organisms formerly identified as strains of paenibacillus pulvifaciens group ii were reclassified as members of the species brevibacillus agri. the reclassification was based on phylogenetic analysis of 16s rrna gene sequences from selected bacillaceae. the analysis strongly supported placement of group ii strains in the genus brevibacillus. high dna relatedness of 87-100% and close phenotypic similarity demonstrated that group ii strains were members of the species brevibacillus agri. | 1997 | 9058544 |
| molecular cloning and nucleotide sequencing of biof (7-keto-8-amino pelargonic acid synthetase), bioc and biod (dethiobiotin synthetase) genes of erwinia herbicola. | the biotin operon of erwinia herbicola eho 10 was cloned and characterized by complementation of e. coli biotin mutants. the operon was found to contain five genes arranged in the order, bioabfcd. the nucleotide sequences of biof (7-keto-8-aminopelargonic acid synthetase), bioc and biod (dethiobiotin synthetase) were determined and analyzed. the nucleotide sequences and deduced amino acid sequences of biofcd were compared with the corresponding sequences from escherichia coli, bacillus sphaericu ... | 1997 | 9063571 |
| site-directed mutagenesis of the aspartokinase gene lysc and its characterization in brevibacterium flavum. | using overlap extension polymerase chain reaction (pcr), five transformants of escherichia coli containing site-directed mutagenized lysc beta gene were generated and analysed. exchange of c to a and c to t at nucleotide 1118 of the mutated lysc beta gene causes a substitution of serine301 in the wild-type enzyme for tyrosine301 and phenylalanine301 in the mutant enzymes, respectively. enzyme assays showed that brevibacterium flavum cells harbouring psumn18 with mutated lysc beta genes exhibited ... | 1997 | 9080702 |
| construction and characterization of new corynebacterial plasmids carrying the alpha-amylase gene. | novel corynebacterial plasmids carrying alpha-amylase gene from bacillus have been constructed. the level of alpha-amylase expression depends on the size of the vector. the highest expression levels were measured in brevibacteria harboring pa61 plasmid. | 1996 | 9090819 |
| temperature-sensitive mutants of corynebacterium ammoniagenes atcc 6872 with a defective large subunit of the manganese-containing ribonucleotide reductase. | chemical mutagenesis of the nucleotide-producing strain corynebacterium ammoniagenes atcc 6872 with n-methyl-n-nitro-n-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. a rapid assay for the allosterically regulated mn-ribonucleotide reductase (rrase) was developed with nucleotide-permeable cells of c. ammoniagenes in order to screen for possible defects in dna precursor biosynthesis at elevated temperature. three mutants (ch 31, ch 32, and ch 33) ... | 1997 | 9094230 |
| antimicrobial susceptibility patterns of some recently established coryneform bacteria. | the susceptibility patterns of 480 isolates representing six recently defined species of coryneform bacteria (corynebacterium amycolatum [n = 101], corynebacterium auris [n = 48], corynebacterium glucuronolyticum [n = 86], brevibacterium casei [n = 50], dermabacter hominis [n = 49], and turicella otitidis [n = 146]) to 17 antimicrobial agents were determined by an agar dilution method. most significantly, for c. amycolatum strains the mics at which 90% of isolates are inhibited were > or = 32 mi ... | 1996 | 9124857 |
| a dtsr gene-disrupted mutant of brevibacterium lactofermentum requires fatty acids for growth and efficiently produces l-glutamate in the presence of an excess of biotin. | a dtsr gene encoding a homolog of the beta subunit of some biotin-containing enzymes suppresses a detergent-sensitive mutation of brevibacterium lactofermentum (e. kimura et al., 1996, biosci. biotech. biochem. 60, 1565-1570), which has been used for the fermentative production of l-glutamate. when the dtsr gene was disrupted, the organism exhibited strict fatty acid auxotrophy; oleate or oleate ester, but not palmitate ester or stearate ester, supported the growth of the delta dtsr mutant. immu ... | 1997 | 9168981 |
| specificity of an extracellular proteinase from brevibacterium linens atcc 9174 on bovine beta-casein. | the specificity of the extracellular proteinase from brevibacterium linens atcc 9174 on bovine beta-casein was studied. hydrolysis was monitored over time by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) and urea-page. the major ph 4.6-soluble peptides were isolated by high-performance liquid chromatography and identified by n-terminal amino acid sequencing and mass spectrometry. the major sites of hydrolysis were ser-18-ser-19, glu-20-glu-21, gln-56-ser-57, gln-72-asn-73, leu ... | 1997 | 9172371 |
| cloning, overexpression, and mutagenesis of the gene for homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum. | homoprotocatechuate (hpca, 3,4-dihydroxyphenylacetate) is a central intermediate for the bacterial degradation of aromatic compounds. homoprotocatechuate 2,3-dioxygenase (hpcd) catalyzes the key ring cleavage step in the metabolism of hpca by the gram (+) bacterium brevibacterium fuscum to yield alpha-hydroxy-delta-carboxymethyl cis-muconic semialdehyde. a genomic dna library of b. fuscum was constructed in escherichia coli using a cosmid vector and screened by spraying the cells with hpca. one ... | 1997 | 9179284 |
| the brevibacterium albidum gene encoding the arginine trnaccg complements the growth defect of an escherichia coli strain carrying a thermosensitive mutation in the rnpa gene at the nonpermissive temperature. | the escherichia coli rnpa gene encodes c5 protein, the protein component of rnase p. the rnpa49 mutation renders the c5 protein thermosensitive, which results in thermosensitivity of rnase p function. the chromosomal dna region from brevibacterium albidum that complements the rnpa49 mutation was analysed. the gene capable of complementing the growth defect of an rnpa49 mutant strain at nonpermissive temperature was identified as the gene for an arginine trna with anticodon ccg by a deletion anal ... | 1997 | 9180701 |
| a direct stereoselective synthesis of 7 beta-hydroxytestosterone. | although 7 beta-hydroxytestosterone is a known product of hepatic androgen metabolism, there are no published methods for its chemical synthesis except from the equally difficult to obtain 7 beta-hydroxy-4-androstene-3,17-dione. we found that several seemingly straightforward routes for its synthesis failed. consequently, we tried to produce 7 beta-hydroxytestosterone by enzymatic oxidation of 5-androstene-3 beta, 7 beta, 17 beta-triol with cholesterol oxidase (brevibacterium sp.), a procedure p ... | 1997 | 9185296 |
| [microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ... | 1996 | 9190234 |
| [microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ... | 1996 | 9190236 |
| identification of an acetolactate synthase small subunit gene in two eukaryotes. | acetolactate synthase catalyses the first step in branched-chain amino acid biosynthesis. the bacterial enzyme contains two large and two small subunits but there is only limited and circumstantial evidence for a small subunit in the eukaryotic enzyme. here this evidence is summarised and protein sequences of two putative eukaryotic small subunits, from a yeast and a red alga, are presented. | 1997 | 9197540 |
| broviac catheter-related bacteraemias due to unusual pathogens in children with cancer: case reports with literature review. | among 102 episodes of intravenous catheter related bacteraemias documented between january 1989 and july 1996 in children receiving antineoplastic chemotherapy or bone marrow transplantation at g. gaslini children's hospital, genoa, italy, were identified seven episodes due to unusual pathogens: bacillus circulans, bacillus licheniformis, brevibacterium casei, flavimonas oryzihabitans, porphyromonas asaccharolytica, comamonas acidovorans and agrobacterium radiobacter. susceptibility to different ... | 1997 | 9200028 |
| analysis of biotin biosynthesis pathway in coryneform bacteria: brevibacterium flavum. | 1997 | 9211286 | |
| the biotransformation of t-butylacetonitrile and its boron-containing analogue trimethylamine-cyanoborane by brevibacterium r312. | the ability of the nitrile hydratase/amidase system from brevibacterium r312 to biotransform tert-butylacetonitrile was studied with a view to their utilisation in the production of novel amino acids from isostructural compounds. brevibacterium r312 was able to transform nitriles with this structure; however, the wide spectrum amidase from this organism was unable to biotransform the corresponding amide to the carboxylic acid. | 1997 | 9238109 |
| cloning, sequencing, and characterization of the ftsz gene from coryneform bacteria. | taking advantage of highly conserved domains present in the ftsz genes from escherichia coli, rhizobium meliloti, and bacillus subtilis, we designed degenerate oligonucleotides (oligos) corresponding to these regions. these oligos were used as primers in pcr in order to amplify dna sequences from brevibacterium flavum mj233 chromosomal dna. the pcr product was used as a probe to recover genomic fragments from a lambda library of br. flavum mj233. the complete nucleotide sequence (nt) of the clon ... | 1997 | 9240446 |
| microbiological composition of raw milk from selected farms in the camembert region of normandy. | raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, yersinia enterocolitica and campylobacters. total bacterial counts and somatic cell counts were measured. lactococci, lactobacilli, dextran-producing leuconostocs, brevibacterium linens, yeasts and moulds, staphylococcus aureus and other micrococcaceae, pseudomonas, coliforms, escherichia coli, enterococci, clostridium perfringens and spores of anaerobic lactate-fermenting bacteria were also counted. pseudomonas (2000 c ... | 1997 | 9246770 |
| heterologous expression and biochemical characterization of two functionally different type i fatty acid synthases from brevibacterium ammoniagenes. | the coryneform bacterium, brevibacterium ammoniagenes, contains two structurally related but functionally differentiated type i fatty acid synthases, fas-a and fas-b. isolation of homogeneous preparations of both enzymes was achieved by constructing specific fasa and fasb expression systems. in b. ammoniagenes, insertional mutagenesis of fasb allowed the specific production of enzymatically active fas-a. the corresponding fasa mutant was not suited for fas-b purification as the level of this enz ... | 1997 | 9249036 |
| genetic identification of members of the genus corynebacterium at genus and species levels with 16s rdna-targeted probes. | 16s rrna gene-targeted probes were designed for the identification of corynebacteria at the genus and species levels. the genus-specific probe hybridized all clinically important members of the genus corynebacterium and could distinguish them from other coryneform bacteria and phylogenetically related high g+c% gram-positive bacteria, including actinomyces, rhodococcus, gordona, nocardia, streptomyces, brevibacterium and mycobacterium. the species-specific probes for c. jeikeium and c. diphtheri ... | 1997 | 9251056 |
| transcriptional analysis of the siga and sigb genes of brevibacterium lactofermentum. | transcription of the siga and sigb genes of brevibacterium lactofermentum, encoding the principal sigma factors of rna polymerase, has been investigated by northern blot and primer extension analysis. northern hybridizations revealed that siga is transcribed as a monocistronic mrna of 1.7 kb and sigb gives two transcripts of 1.1 and 1.5 kb. similar transcription patterns of siga and sigb genes in nutrient-rich medium were observed; transcripts of both genes occurred simultaneously throughout the ... | 1997 | 9252580 |
| relationship between the glutamate production and the activity of 2-oxoglutarate dehydrogenase in brevibacterium lactofermentum. | enzyme activities of 2-oxoglutarate dehydrogenase complex and glutamate dehydrogenase of wild type brevibacterium lactofermentum, one of the typical glutamate-producing coryneform bacteria, were investigated by using cells cultured under glutamate-productive and glutamate-non-productive conditions. significant reduction of the former enzyme activity was observed in the cells under the several glutamate-productive conditions, namely, in the cells cultured in media containing a) limited concentrat ... | 1997 | 9255973 |
| antibacterial and hemolytic activities of linenscin oc2, a hydrophobic substance produced by brevibacterium linens oc2. | linenscin oc2 is an antibacterial substance produced by the orange cheese coryneform bacterium brevibacterium linens oc2. it inhibits the growth of gram-positive bacteria but it is inactive against gram-negative bacteria. the intact outer membrane of gram-negative bacteria was shown to be an effective permeability barrier against linenscin oc2. at high dosage the effect of linenscin oc2 was bacteriolytic on listeria innocua. bacteriostasis was observed at low dosage and peptidoglycan biosynthesi ... | 1997 | 9271855 |
| cryptic plasmid pka22 isolated from the naphthalene degrading derivative of rhodococcus rhodochrous ncimb13064. | cryptic plasmids were found in rhodococcus rhodochrous ncimb13064 derivatives which had lost the ability to utilize short-chain 1-chloroalkanes (chain length c3-c10) and had acquired the ability to degrade naphthalene. the reversions of these derivatives to the original phenotype were accompanied by the loss of the cryptic plasmids. the 4969-bp pka22 plasmid was cloned in escherichia coli and sequenced. this plasmid encodes a putative 33,200-da protein which contains motifs typical of theta repl ... | 1997 | 9281496 |
| crystal structure and resonance raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate. | the crystal structure of the anaerobic complex of pseudomonas putida protocatechuate 3,4-dioxygenase (3,4-pcd) bound with the alternative substrate, 3,4-dihydroxyphenylacetate (hpca), is reported at 2.4 a resolution and refined to an r factor of 0.17. formation of the active site fe(iii).hpca chelated complex causes the endogenous axial tyrosinate, tyr447 (147beta), to dissociate from the iron and rotate into an alternative orientation analogous to that previously observed in the anaerobic 3,4-p ... | 1997 | 9298971 |
| identification, isolation and biochemical characterization of a phosphopantetheine:protein transferase that activates the two type-i fatty acid synthases of brevibacterium ammoniagenes. | upon heterologous expression of the brevibacterium ammoniagenes type-i fatty acid synthase fas-a in escherichia coli, only the pantetheine-free apoenzyme is synthesized. activation of fas-a to its holoform was achieved by transformation with a second b. ammoniagenes gene, ppt1, encoding a type-i fas-specific phosphopantetheine transferase. ppt1 was identified as a coding sequence located immediately downstream of the second fas gene present on the b. ammoniagenes genome, fasb. due to this linkag ... | 1997 | 9346306 |
| structural analysis of the 6 kb cryptic plasmid pfaj2600 from rhodococcus erythropolis ni86/21 and construction of escherichia coli-rhodococcus shuttle vectors. | the complete nucleotide sequence of the 5936 bp cryptic plasmid pfaj2600 from rhodococcus erythropolis ni86/21 was determined. based on the characteristics of its putative replication genes, repa and repb, pfaj2600 was assigned to the family of pal5000-related small replicons identified in mycobacterium (pal5000), corynebacterium (pxz10142), brevibacterium (prbl1), bifidobacterium (pmb1) and neisseria (pjd1). the replication systems of these plasmids show striking similarities to the ones used b ... | 1997 | 9353918 |
| characterization of guanosine kinase from brevibacterium acetylicum atcc 953. | guanosine kinase (gkase) activity was identified in a cell-free extract of brevibacterium acetylicum atcc 953. we have purified the enzyme 4000-fold from the cell-free extract to apparent homogeneity. molecular weight of 71,300 and 36,300 determined by gel filtration and sodium dodecyl sulfate gel electrophoresis, respectively, revealed that the enzyme is a dimer molecule. maximum activity of the gkase was attained when the magnesium/atp concentration ratio was close to 1. the gkase activity was ... | 1997 | 9357959 |
| sequence analysis of functional regions of homoserine dehydrogenase genes from l-lysine and l-threonine-producing mutants of brevibacterium lactofermentum. | the homoserine dehydrogenase (hd) genes from brevibacterium lactofermentum lysine- and threonine-producing mutants were cloned, using the polymerase chain reaction, and sequenced. we found the amino acid substitutions, val104ile in the lysine-producing mutants in which hd may cause leaky mutation and ser393phe in the threonine-producing mutant with feedback-insensitive hd. | 1997 | 9362124 |
| cloning, sequencing and expression of the gene encoding elongation factor p in the amino-acid producer brevibacterium lactofermentum (corynebacterium glutamicum atcc 13869). | the brevibacterium lactofermentum ef-p gene, encoding the elongation factor protein p, was cloned and sequenced. according to dna sequence analysis of this gene, the b. lactofermentum ef-p protein consists of 187 amino acids with a calculated molecular weight of 20,584. southern hybridization of an internal fragment of the ef-p gene from b. lactofermentum with chromosomal dnas from different microorganisms reveals that it is a unique gene product in b. lactofermentum and corynebacterium glutamic ... | 1997 | 9370284 |
| molecular cloning and transcriptional analysis of a guanosine kinase gene of brevibacterium acetylicum atcc 953. | the brevibacterium acetylicum gsk gene, which encodes guanosine kinase (atp:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the n-terminal amino acid sequence of the purified protein. the cloned chromosomal fragment containing the gsk gene was sequenced and shown to encode a polypeptide of 303 amino acids with a molecular mass of 32,536 da, which is in good agreement with the measured molecular weight of the purified enzyme. rec ... | 1997 | 9371440 |
| one- and two-dimensional eseem spectroscopy of flavoproteins. | one- and two-dimensional (1d and 2d) electron spin echo envelope modulation (eseem) spectroscopy was applied to study the flavin cofactors in the neutral semiquinone states of flavodoxin and ferredoxin-nadp+ reductase (fnr) from the cyanobacterium anabaena pcc 7119, and the anionic semiquinone state of cholesterol oxidase from brevibacterium sterolicum. high-resolution crystal structures are available for all these proteins. three- and 4-pulse eseem and hyperfine sublevel correlation spectroscop ... | 1997 | 9398281 |
| growth reduction of listeria spp. caused by undefined industrial red smear cheese cultures and bacteriocin-producing brevibacterium lines as evaluated in situ on soft cheese. | the undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of listeria spp. in some cases, these microbial consortia inhibit listeria almost completely. from such undefined industrial cheese-ripening floras, linocin m18-producing (lin+) (n. valdés-stauber and s. scherer, appl. environ. microbiol. 60:3809-3814, 1994) and -nonproducing brevibacterium linens strains were isolated and used ... | 1997 | 9406400 |
| characterization of cholesterol oxidase from streptomyces hygroscopicus and brevibacterium sterolicum. | the fad-containing enzyme cholesterol oxidase catalyzes the oxidation and isomerization of 3beta-hydroxysteroids having a trans double bond at delta5-delta6 of the steroid ring backbone to the corresponding delta4-3-ketosteroid. two representative enzymes of this family, namely cholesterol oxidase from streptomyces hygroscopicus (sco) and the recombinant enzyme from brevibacterium sterolicum (bco) expressed in escherichia coli, have been characterized herein in their chemical, physical, and bioc ... | 1997 | 9428686 |
| evaluation of the rapid cb plus system for identification of corynebacterium species and other gram-positive rods. | due to the difficulty of identifying corynebacterium spp. with standard methods, we compared them with the rapid cb plus system (remel, lenexa, kans. [formerly innovative diagnostic systems, norcross, ga.]), which consists of 4 carbohydrate and 14 preformed enzyme tests, for the identification of 98 clinical isolates of corynebacterium sp., other coryneforms, listeria monocytogenes, and 17 atcc strains. forty (95%) of 42 strains of corynebacterium spp. were accurately identified to the species l ... | 1998 | 9466773 |
| purification and characterization of the 2-ketoaldonate reductase from brevibacterium ketosoreductum atcc21914. | 2-ketoaldonate reductase, which is involved in ketogluconate catabolism, was purified to homogeneity from brevibacterium ketosoreductum atcc21914. the enzyme was found to catalyze the reduction of 2,5-diketo-d-gluconate to 5-keto-d-gluconate, and to a lesser extent, 2-keto-d-gluconate to d-gluconate, and 2-keto-l-gluconate to l-idonate. the molecular mass of the reductase was 35 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 72 kda by gel filtration, indicating that the nat ... | 1998 | 9501529 |
| glucose oxidase from penicillium amagasakiense. primary structure and comparison with other glucose-methanol-choline (gmc) oxidoreductases. | the complete amino acid sequence of glucose oxidase from penicillium amagasakiense was determined by edman degradation and mass spectrometry of peptide fragments derived from three different specific proteolytic digests and a cyanogen bromide cleavage. the complete sequence of each monomer comprises 587 amino acid residues, contains three cysteine residues, and seven potential n-glycosylation sites, of which at least five were confirmed to be glycosylated. glucose oxidase from p. amagasakiense s ... | 1998 | 9523716 |
| increased expression of brevibacterium sterolicum cholesterol oxidase in escherichia coli by genetic modification. | to improve expression of brevibacterium sterolicum cholesterol oxidase in escherichia coli, we utilized the t7lac promoter and modified the gene to encode the first 21 amino acids with high-expression e. coli codons. these changes resulted in a 60-fold improvement of expression level. n-terminal sequencing revealed that the e. coli produced cholesterol oxidase signal peptide is cleaved 6 amino acids closer to the n-terminus than in b. sterolicum. the recombinant e. coli produced protein is compo ... | 1998 | 9535702 |
| assessment of the role of an omega loop of cholesterol oxidase: a truncated loop mutant has altered substrate specificity. | the function of an active site loop (70-90) of cholesterol oxidase has been ascertained by deleting five contiguous residues (79-83) from the tip of the loop. from the crystal structure of the wild-type enzyme, it appears that this truncation will not significantly perturb the structure of the rest of the enzyme. the uv/vis and cd spectra of the mutant confirm that the enzyme is properly folded with fad bound. the mutant enzyme still transfers 2h from the 4beta-carbon of the intermediate, choles ... | 1998 | 9548964 |
| histamine and tyramine degradation by food fermenting microorganisms. | microorganisms suitable for food fermentation were examined with regard to their potential to degrade histamine and tyramine. out of 64 lactic acid bacteria evaluated in this study, 27 degraded histamine and one tyramine, respectively, with low activity. among 32 strains of brevibacterium linens and coryneform bacteria, 21 exhibited histamine and tyramine oxidase activity. none of 20 strains of staphylococcus carnosus tested degraded histamine or tyramine. one strain out of nine strains of geotr ... | 1998 | 9562873 |
| identification and characterization of a 29-kilodalton protein from mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. | culture filtrate proteins from mycobacterium tuberculosis induce protective immunity in various animal models of tuberculosis. two molecular mass regions (6 to 10 kda and 24 to 36 kda) of short-term culture filtrate are preferentially recognized by th1 cells in animal models as well as by patients with minimal disease. in the present study, the 24- to 36-kda region has been studied, and the t-cell reactivity has been mapped in detail. monoclonal antibodies were generated, and one monoclonal anti ... | 1998 | 9596740 |
| a divalent metal site in the small subunit of the manganese-dependent ribonucleotide reductase of corynebacterium ammoniagenes. | based on its metallo-cofactor, the manganese-dependent ribonucleotide reductase (mn-rrase) responsible for delivery of dna precursors in the mn-requiring gram-positive bacterium corynebacterium (formerly brevibacterium) ammoniagenes atcc 6872 is no longer considered as a simple analogue of the aerobic fe-rrase of escherichia coli but as the prototype of the class iv enzymes (1). deliberate dissociation of the mn-rrase holoenzyme and an improved sample preparation of the dimeric ca2 protein allow ... | 1998 | 9609691 |
| [novel site-specific endonucleases from brevibacterium species]. | new site-specific endonucleases becai and becaii have been detected in brevibacterium species a. endonuclease becaii free from contaminating nonspecific endonucleases, exonucleases, and phosphatases was isolated by column chromatography on phosphocellulose, heparin sepharose, and dna cellulose. it recognizes and cleaves the 5'-gg decreases cc-3' sequence and is a true isoschizomer of haeiii restriction enzyme. the other restriction endonuclease, becai, cleaves ad2 dna at least by 2 sites but not ... | 1998 | 9611761 |
| new shuttle vectors for rhodococcus sp. r312 (formerly brevibacterium sp. r312), a nitrile hydratase producing strain. | two shuttle vectors named prc52 (10.7 kb) and prk52 (12.2 kb) carrying chloramphenicol (cm) and chloramphenicol plus kanamycin (km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pbl13869 (replicon pbl1, 5.8 kb) from brevibacterium lactofermentum atcc13869 with pbr328 e. coli plasmid. transformation of rhodococcus sp. r312 (formerly brevibacterium sp. r312) protoplasts was realised with an efficiency of 28 transformants per micrograms of dna. | 1998 | 9637010 |
| brevibacterium iodinum peritonitis associated with acute urticaria in a capd patient. | 1997 | 9655165 | |
| degradation of histamine and tyramine by brevibacterium linens during surface ripening of munster cheese. | red smear formation during fermentation of munster cheese was started by using three different strains of brevibacterium linens as surface inocula. the cheeses were produced with and without supplementation of histamine and tyramine. after smearing the cheese surface for the first time with b. linens viable counts of 10(7) cfu/g were detected. at the end of the logarithmic growth phase cell numbers increased to 10(10) cfu/g and remained constant during the whole ripening period. during a 4-week ... | 1998 | 9678172 |
| a novel phosphopantetheine:protein transferase activating yeast mitochondrial acyl carrier protein. | in saccharomyces cerevisiae, the low molecular weight acyl carrier protein (acp) of mitochondrial type ii fatty acid synthase (fas) and the cytoplasmic type i fas multienzyme contain 4'-phosphopantetheine as a prosthetic group. sequence alignment studies with the recently isolated phosphopantetheine:protein transferase (pptase), ppt1p, from brevibacterium ammoniagenes revealed the yeast open reading frame, ypl148c, as a potential pptase gene (25% identical and 43% conserved amino acids). in acco ... | 1998 | 9712852 |
| microbial communities of printing paper machines. | the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ... | 1998 | 9717292 |
| conversion of methionine to thiols by lactococci, lactobacilli, and brevibacteria | methanethiol has been strongly associated with desirable cheddar cheese flavor and can be formed from the degradation of methionine (met) via a number of microbial enzymes. methionine gamma-lyase is thought to play a major role in the catabolism of met and generation of methanethiol in several species of bacteria. other enzymes that have been reported to be capable of producing methanethiol from met in lactic acid bacteria include cystathionine beta-lyase and cystathionine gamma-lyase. the objec ... | 1998 | 9726877 |
| purification and characterization of l-methionine gamma-lyase from brevibacterium linens bl2 | l-methionine gamma-lyase (ec 4.4.1.11) was purified to homogeneity from brevibacterium linens bl2, a coryneform bacterium which has been used successfully as an adjunct bacterium to improve the flavor of cheddar cheese. the enzyme catalyzes the alpha,gamma elimination of methionine to produce methanethiol, alpha-ketobutyrate, and ammonia. it is a pyridoxal phosphate-dependent enzyme, with a native molecular mass of approximately 170 kda, consisting of four identical subunits of 43 kda each. the ... | 1998 | 9726878 |
| mode of action of linenscin oc2 against listeria innocua. | linenscin oc2 is a small hydrophobic substance produced by the orange cheese coryneform bacterium brevibacterium linens oc2. linenscin oc2 inhibits growth of gram-negative bacteria with an altered outer membrane permeability and gram-positive bacteria. it is also able to lyse eucaryotic cells. the mode of action of linenscin oc2 on the listeria innocua cytoplasmic membrane and the effects of environmental parameters were investigated. addition of low doses of linenscin oc2 resulted in an immedia ... | 1998 | 9726891 |
| identification, characterization, and chromosomal organization of the ftsz gene from brevibacterium lactofermentum. | the ftsz gene was cloned from the chromosomal dna of brevibacterium lactofermentum by the polymerase chain reaction (pcr) using two oligonucleotides designed from two conserved regions found in most of the previously cloned and sequenced ftsz genes from other microorganisms. ftsz is a single-copy gene in corynebacteria and is located downstream from ftsq and murc, indicating linkage between genes involved in peptidoglycan synthesis (mur genes) and genes involved in cell division (fts genes). the ... | 1998 | 9738885 |
| intracellular changes in ions and organic solutes in halotolerant brevibacterium sp. strain jcm 6894 after exposure to hyperosmotic shock | in the present study we aimed to observe the intracellular responses when there was a hyperosmotic shock with a large shift in ionic strength in nutrient-rich and nutrient-poor external environments in order to clarify the availability of substrates. to do this, we used the halotolerant organism brevibacterium sp. strain jcm 6894, which is able to grow in the presence of a wide range of salt concentrations. hyperosmotic shock was induced by transferring cells in the late exponential phase of gro ... | 1998 | 9758779 |
| carnitine acts as a compatible solute in brevibacterium linens. | carnitine is a trimethyl amino acid found at relatively high concentrations in materials of animal origin. exogenously provided l-carnitine was found to stimulate growth of brevibacterium linens atcc 19391 in media with inhibitory osmotic strength. its osmoprotective ability was as potent as that of glycine betaine. electrophoretic and spectroscopic (nmr) analysis showed that this compound is only transiently accumulated, but in significant amounts, by b. linens under hyperosmotic stress and is ... | 1998 | 9766223 |
| pbla8, from brevibacterium linens, belongs to a gram-positive subfamily of cole2-related plasmids. | a 3.1 kb dna fragment from pbla8, a brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. this fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, repa and repb, both essential for replication. based on structural similarities and a strong conservat ... | 1998 | 9802024 |
| molecular characterization of guanosine kinase gene from a facultative alkalophile, exiguobacterium aurantiacum atcc 35652. | guanosine kinase activity was identified in an alkalophile, exiguobacterium aurantiacum atcc 35652, and the gsk gene encoding it was isolated as a homologous fragment of the brevibacterium acetylicum gsk. the cloned chromosomal fragment contained an open reading frame which encodes a polypeptide of 308 amino acids with a molecular mass of 33349 da. the ex. aurantiacum gsk shows strong sequence similarity to b. acetylicum gsk, but possesses five additional amino acids at the c-terminus. an mr 392 ... | 1998 | 9804993 |
| in vitro activities of ketolide hmr 3647, macrolides, and clindamycin against coryneform bacteria. | the in vitro activity of ketolide hmr 3647 against coryneform bacteria isolated from clinical samples was evaluated. except against corynebacterium jeikeium and c. urealyticum, hmr 3647 showed high activity against corynebacterium spp., being more active than 14- and 16-membered macrolides, azithromycin, or clindamycin. hmr 3647 also had high in vitro activity against brevibacterium spp. and listeria monocytogenes. | 1998 | 9835530 |
| single-molecule enzymatic dynamics. | enzymatic turnovers of single cholesterol oxidase molecules were observed in real time by monitoring the emission from the enzyme's fluorescent active site, flavin adenine dinucleotide (fad). statistical analyses of single-molecule trajectories revealed a significant and slow fluctuation in the rate of cholesterol oxidation by fad. the static disorder and dynamic disorder of reaction rates, which are essentially indistinguishable in ensemble-averaged experiments, were determined separately by th ... | 1998 | 9836635 |