Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular genetic analysis of a thioredoxin gene from thiobacillus ferrooxidans. | the thiobacillus ferrooxidans thioredoxin gene, trxa, was isolated by its ability to complement an escherichia coli gsha trxa mutant which was otherwise unable to grow on minimal medium lacking glutathione. the t. ferrooxidans thioredoxin also enabled the in vivo reduction by e. coli of methionine sulfoxide to methionine, as well as the in vitro reduction of insulin. when present in e. coli, the t. ferrooxidans thioredoxin supported the replication of phage t7, but not the growth of phage m13. t ... | 1995 | 7496529 |
| characterisation and amino acid sequence of cytochrome c-550 from thiosphaera pantotropha. | a cytochrome c-550, with mid-point potential +265 mv, has been purified from thiosphaera pantotropha. the cytochrome was recognised by antibodies to paracoccus denitrificans cytochrome c-550, but the two proteins were not immunologically identical. amino acid sequencing of the cytochrome c-550 showed 85.9% and 95.5% identities, respectively, with the cytochromes c-550 of p. denitrificans and thiobacillus versutus; these are amongst the highest values reported for similarities between class i c-t ... | 1994 | 7508392 |
| cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments. | a range of autotrophic and heterotrophic enrichment cultures were established to determine the cultural bacterial diversity present in samples obtained from the acidic runoff of a chalcocite overburden heap and from laboratory-scale (1- to 4-liter) batch and continuous bioreactors which were being used for the commercial assessment of the bioleachability of zinc sulfide ore concentrates. strains identified as thiobacillus ferrooxidans, thiobacillus thiooxidans, "leptospirillum ferrooxidans," and ... | 1994 | 7517131 |
| thiobacillus ferrooxidans tyrosyl-trna synthetase functions in vivo in escherichia coli. | the tyrosyl-trna synthetase gene (tyrz) from thiobacillus ferrooxidans, an acidophilic, autotrophic, gram-negative bacterium that participates in bioleaching of minerals, was cloned and sequenced. the encoded polypeptide (tyrrz) is 407 amino acids in length (molecular mass; 38 kda). the predicted protein sequence has an extensive overall identity (44%) to the sequence of the protein encoded by the bacillus subtilus tyrz gene, one of the two genes encoding tyrosyl-trna synthetases in this microor ... | 1994 | 7517395 |
| phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of roseococcus thiosulfatophilus gen. nov., sp. nov., erythromicrobium ramosum gen. nov., sp. nov., and erythrobacter litoralis sp. nov. | we analyzed the 16s ribosomal dnas of three obligately aerobic, bacteriochlorophyll a-containing bacteria, "roseococcus thiosulfatophilus," "erythromicrobium ramosum," and new isolate t4t (t = type strain), which was obtained from a marine cyanobacterial mat. "roseococcus thiosulfatophilus" is a member of the alpha-1 subclass of the proteobacteria and is moderately related to rhodopila globiformis, thiobacillus acidophilus, and acidiphilium cryptum (level of sequence similarity, 90%). "erythromi ... | 1994 | 7520734 |
| the structure of the o-specific polysaccharide from thiobacillus ferrooxidans ifo 14262. | lipopolysaccharide (lps) was isolated from thiobacillus ferrooxidans ifo 14262 by the hot phenol-water extraction procedure. the o-specific polysaccharide, liberated from lps by mild acetic acid hydrolysis, had a branched pentasaccharide repeating-unit composed of d-glucose, l-rhamnose, d-rhamnose, and 3-o-methyl-l-rhamnose in approximate molar ratios of 2:1:1:1. on the basis of methylation analysis, 1h and 13c nmr spectroscopy, including 2d shift-correlated (cosy) and 1d noe spectroscopy, the s ... | 1994 | 7522125 |
| characterization of thiobacillus caldus sp. nov., a moderately thermophilic acidophile. | two isolates of a novel, moderately thermophilic thiobacillus species have been studied. the isolates, ku and bc13, are gram-negative, motile bacteria having a ph optimum for growth of 2-2.5 and an optimum growth temperature of 45 degrees c. both isolates are capable of chemolithotrophic growth on reduced sulfur substrates. they can also use molecular hydrogen as an electron donor. these two isolates can grow mixotrophically with sulfur or tetrathionate and yeast extract or glucose. the g+c cont ... | 1994 | 7533596 |
| the structure of the o-specific polysaccharide from thiobacillus sp. ifo 14570, with three different diaminopyranoses forming the repeating unit. | the o-specific polysaccharide, liberated by mild acid hydrolysis of the lipopolysaccharide (lps-p) from thiobacillus sp. ifo14570 as isolated from the phenol phase after phenol-water extraction, is shown to have a linear trisaccharide repeating-unit containing three different diamino sugars, namely 2,4-diacetamido-2,4-dideoxyglucuronic acid, 2-acetamidino-4-acetamido-2,4,6-trideoxyglucopyranose, and 2,3-diacetamido-2,3-dideoxyglucuronic acid in the molar ratio of 1:1:1. on the basis of 1h and 13 ... | 1995 | 7539718 |
| paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of thiobacillus versutus to the genus paracoccus as paracoccus versutus comb. nov. with emendation of the genus. | a facultatively chemolithotrophic thiocyanate-degrading bacterium, strain thi 011t, which was previously isolated from activated sludge and tentatively named thiobacillus sp., was studied taxonomically and phylogenetically. this bacterium utilizes thiocyanate as sole energy source and the specific growth rate for chemolithoautotrophic growth with thiocyanate was 0.059 h-1. molecular phylogenetic relationships of strain thi 011t to thiobacillus versutus and members of the genus paracoccus were el ... | 1995 | 7545513 |
| evidence for a methylammonium-binding site on methylamine dehydrogenase of thiobacillus versutus. | the nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (madhox) from thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of madhox as do the monovalent cations cs+, rb+, and nh4+. like the monovalent cations, trimethylamine also competitively inhibits the reduction of madhox by methylamine. rapid-scan experiments show that within the first few milliseconds of the reacti ... | 1995 | 7548050 |
| x-ray absorption studies and homology modeling define the structural features that specify the nature of the copper site in rusticyanin. | rusticyanin, a blue copper protein, possessing the highest redox potential among this class of proteins and a high stability at acidic ph reveals homology with the c-terminal end of the other single copper containing blue proteins and an interesting homology to parts of the blue copper domain of the multi-copper proteins such as the nitrite reductases. extended x-ray absorption fine structure (exafs) data at ph 2.0 reveal that cu is ligated to two his and a cys in the inner coordination sphere, ... | 1995 | 7599131 |
| gene synthesis, high-level expression, and mutagenesis of thiobacillus ferrooxidans rusticyanin: his 85 is a ligand to the blue copper center. | an artificial gene of the blue copper protein rusticyanin from thiobacillus ferrooxidans was constructed from eight overlapping oligonucleotides in a recursive "one-pot" polymerase chain reaction. the gene was placed behind the t7/lacor promoter of pet24a and expressed in escherichia coli as a soluble protein. a purification scheme involving a ph titration step, cation-exchange chromatography, and reverse-phase hplc separation provided yields of the apoprotein ranging from 70 to 100 mg/l of cell ... | 1995 | 7639845 |
| identification and activity of two insertion sequence elements in rhodococcus sp. strain igts8. | two putative insertion sequence (is) elements, is1166 and is1295, were identified on a plasmid present in rhodococcus sp. igts8. four copies of is1166 were present in strain igts8: one copy on each of two separate plasmids and two copies on a third plasmid or in the chromosome. of eight rhodococci tested, only r. zopfii contained a copy of an is1166-like element. two mutants of strain igts8 were isolated in which an additional copy of is1166 was present, suggesting that at least one copy of this ... | 1995 | 7642133 |
| integration of heterologous dna into the genome of paracoccus denitrificans is mediated by a family of is1248-related elements and a second type of integrative recombination event. | all members of the is1248 family residing in the genome of paracoccus denitrificans have been isolated by using a set of insertion sequence entrapment vectors. the family consists of five closely related members that integrate the entrapment vectors at distinct sites. one of these, is1248b, was sequenced and, except for a single base change, shown to be identical to the previously isolated is1248a. southern analysis of genomic dna with labeled is1248 revealed different hybridization patterns for ... | 1995 | 7642505 |
| the genomic region of rbcls in synechococcus sp. pcc 7942 contains genes involved in the ability to grow under low co2 concentration and in chlorophyll biosynthesis. | several genes involved in the ability of synechococcus sp. pcc 7942 to grow under different co2 concentrations were mapped in the genomic region of rbcls (the operon encoding the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase). insertion of a cartridge encoding kanamycin resistance within open reading frame (orf) 78, designated ccmj, located 7 kb upstream of rbcls, resulted in a kanamycin-resistant, high-co2-requiring mutant, m3, which does not contain normal carboxy ... | 1995 | 7659748 |
| profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rrna. | we describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. this technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16s rrna, all the same length, by denaturing gradient gel electrophoresis (dgge). dgge analysis of different microbial communities demonstrated the presence of up to 10 distinguishable bands in the separation pattern, which were most likely derived from as many different species con ... | 1993 | 7683183 |
| biological production and consumption of gaseous organic sulphur compounds. | 1994 | 7698397 | |
| isolation and study of two strains of leptospirillum-like bacteria from a natural mixed population cultured on a cobaltiferous pyrite substrate. | two strains of leptospirillum-like bacteria, l6 and l8, have been isolated from a mixed inoculum, also containing thiobacillus ferrooxidans and t. thiooxidans, cultured for one year with a colbaltiferous pyrite as energy substrate in a 100 l continuous bioleaching laboratory unit. several physiological properties of the strains are described. the vibrio-shaped microorganisms grew at ph values lower than 1.3. their growth rate was maximum between 2.5 and 8.0 g l1 ferrous iron. the optimal growth ... | 1994 | 7710275 |
| stability of copper tolerance in thiobacillus ferrooxidans. | a strain of thiobacillus ferrooxidans mal-4-1 was adapted to grow at higher concentrations of copper by repeated subculturing in the presence of increasing levels of added cupric ions in 9k medium. the strains adapted to copper were found to be more efficient in bioleaching of copper from concentrates. when copper tolerant strains were back cultured repeatedly in 9k medium without cupric ions, the initially developed metal tolerance was observed to be lost. this indicates that the copper toleran ... | 1994 | 7710276 |
| isolation and characterization of the replicon of a thiobacillus intermedius plasmid. | the replicon of a cryptic thiobacillus intermedius plasmid (ptik12) has been isolated and sequenced. functional analysis of deletion subclones in escherichia coli localized the replicon to a 3.5-kb region of dna. sequencing of this region identified a 30-bp a-t-rich potential stem-loop structure. in addition, an 11-bp direct repeat, an 11-bp inverted repeat, and a 16-bp inverted repeat were observed at the stem-loop structure. also found in the replicon was a series of four tandem direct repeats ... | 1995 | 7753904 |
| identification of the pura gene encoding adenylosuccinate synthetase in thiobacillus ferrooxidans. | the pura gene of thiobacillus ferrooxidans encoding adenylosuccinate synthetase [ec 6.3.4.4] was identified in the upstream region of the iro gene encoding fe(ii)-oxidase (j. biol. chem 267:11242-11247, 1992). the pura gene consisted of 1290 base-pairs, which translated into a 29-amino-acid protein. the gene is functionally active, because it is able to complement an escherichia coli pura-deficient strain. the deduced gene product has a high degree (60.9%) of sequence identity with that (432 aa) ... | 1993 | 7763499 |
| transformation of the acidophilic heterotroph acidiphilium facilis by electroporation. | we constructed a cloning vector for use in the acidophilic heterotroph acidiphilium facilis. the vector pah101 (8.8 kb) was constructed from a 6.1 kb restriction fragment of the acidiphilium plasmid pah1 and a puc19 carrying a beta-lactamase gene. the antibiotic resistance gene was efficiently expressed in a. facilis. several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 x 10(3) transformants per microgram of plasmid dn ... | 1993 | 7764274 |
| a dna region that complements on escherichia coli cysg mutation in thiobacillus ferrooxidans. | thiobacillus ferrooxidans ap19-3 has a novel nadh-dependent sulfite reductase in the periplasmic space. the gene responsible for the appearance of nadh-dependent sulfite reductase activity was cloned into a vector plasmid pbr322 to give a 5.7-kb hybrid plasmid, pths1, which contains a 1.3-kb dna fragment of t. ferrooxidans ap19-3. when pths1 was used to transform sulfite reductase deficient e. coli mutants, strain at2455 (cysg), jm246 (cysi), and at2427 (cysj), it complemented only the e. coli c ... | 1995 | 7772840 |
| complete 13c assignments for recombinant cu(i) rusticyanin. prediction of secondary structure from patterns of chemical shifts. | complete resonance assignments for the 13c spectrum of reduced (cu(i)) rusticyanin have been made using 13c, 15n doubly labeled recombinant material. the reported assignments include those for the carboxyl and carbonyl carbon atoms and protonated aromatic ring carbons, and were obtained using a variety of 2- and 3d inverse-detected nmr experiments, including 13c, 15n, 1h triple resonance experiments and hcch-cosy and -tocsy. backbone carbonyl assignments were obtained using 3d hnco and hcaco spe ... | 1995 | 7774711 |
| introduction of a cua site into the blue copper protein amicyanin from thiobacillus versutus. | the c-terminal loop of the blue copper protein amicyanin, which contains three of the four active site ligands, has been replaced with a cua binding loop. the purple protein produced has visible and epr spectra identical to those of a cua centre. recent evidence strongly suggests that the cua centre of cytochrome c oxidase and the a centre of nitrous oxide reductase are similar and are both binuclear. it therefore follows that the purple amicyanin mutant created here also possesses a binuclear c ... | 1995 | 7774723 |
| ethanolamine utilization in salmonella typhimurium: nucleotide sequence, protein expression, and mutational analysis of the ccha cchb eute eutj eutg euth gene cluster. | a fragment of the salmonella typhimurium ethanolamine utilization operon was cloned and characterized. the 6.3-kb nucleotide sequence encoded six complete open reading frames, termed ccha, cchb, eute, eutj, eutg, and euth. in addition, the nucleotide sequences of two incomplete open reading frames, termed eutx and euti, were also determined. comparison of the deduced amino acid sequences and entries in the genbank database indicated that euti encodes a phosphate acetyltransferase-like enzyme. th ... | 1995 | 7868611 |
| [application of immunologic methods to the analysis of bio-leaching bacteria]. | pure cultures of thiobacillus ferrooxidans and mixed cultures of thiobacillus ferrooxidans and leptospirillum ferrooxidans isolated from the matahambre mine (cuba) were used to fit immunodiffusion and immunoelectron microscopy to the study of iron oxidizing bacteria. the possibilities, advantages and limits of those techniques have been studied from both the identification and the serological characterization points of view. finally, the efficiency of these methods was tested by applying them to ... | 1994 | 7873106 |
| [preservation of acidophilic bacteria]. | 1994 | 7873107 | |
| development and application of a monoclonal antibody against thiothrix spp. | historically, methods used to identify thiothrix spp. in environmental samples have been inadequate because isolation and identification procedures are time-consuming and often fail to separate thiothrix spp. from other filamentous microorganisms. we described a monoclonal antibody-based enzyme-linked immunosorbent assay (elisa) procedure which was used to identify thiothrix spp. in wastewater, artesian springs, groundwater, and underwater subterranean samples. the elisa utilized monoclonal anti ... | 1995 | 7887596 |
| n-terminal heterogeneity of methylamine dehydrogenase from thiobacillus versutus. | the n-terminal processing of madh from the bacterium t. versutus and the n-terminal heterogeneity of the isolated alpha subunit of the alpha 2 beta 2 protein complex was demonstrated by a combination of edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate mass determination of the homogeneous subunit by the technique of electrospray ionisation mass spectrometry. from this study, it appears that the corresponding gene of the alpha sub ... | 1993 | 7901050 |
| mutagenesis of the conserved lysine 14 of cytochrome c-550 from thiobacillus versutus affects the protein structure and the electron self-exchange rate. | the lysine residue k14 of cytochrome c-550 of thiobacillus versutus has been mutated to a glutamine (q) and a glutamate (e) residue. these mutations have a minimal effect on the pka for replacement of the methionine ligand (the "alkaline transition"), indicating that a presumptive salt bridge between k14 and e11 does not help stabilize the native form. this is in contrast with mitochondrial cytochrome c, where the homologous k13 forms a structurally important salt bridge with glutamate 90. the n ... | 1993 | 7903553 |
| preliminary study of treatment of sulphuric pickling water waste from steelmaking by bio-oxidation with thiobacillus ferrooxidans. | this report looks at the laboratory-scale recovery of iron oxides (alpha fe2o3 type) through bio-oxidation with thiobacillus ferrooxidans of the ferrous sulphate contained in steel industry sulphuric pickling liquors. this is done by calcining iron sulphates and iron and ammonium sulphates obtained from the crystallization of the oxidized solution. the products of the bacterial reaction and the iron oxides are then studied according to calcination temperature. the process carried out produced 50 ... | 1994 | 7917427 |
| the effects of ph and cations on the spectral and kinetic properties of methylamine dehydrogenase from thiobacillus versutus. | the catalytic parameters of thiobacillus versutus methylamine dehydrogenase (madh) with the physiological substrates methylamine and amicyanin show a ph profile that is quite different from the one found in commonly used assays with artificial electron acceptors. the optimum at ph 7.5, observed for kcat in the latter case, is absent with amicyanin as the reoxidizing substrate. with amicyanin kcat scarcely depends on ph; the same is true for the maximal rate of reduction of madh by methylamine (k ... | 1994 | 7918441 |
| identification and sequence analysis of the soxb gene essential for sulfur oxidation of paracoccus denitrificans gb17. | the coding region for lithotrophic sulfur oxidation (sox) in paracoccus denitrificans gb17 was identified by isolation of a transposon tn5-mob mutant with a sox- phenotype (strain tp19). the corresponding wild-type region was cloned previously (g. mittenhuber, k. sonomoto, m. egert, and c. g. friedrich, j. bacteriol. 173:7340-7344, 1991). sequence analysis of a 2.5-kb subclone that complemented strain tp19 revealed that tn5-mob was inserted into a coding region for a 553-amino-acid polypeptide n ... | 1994 | 7928987 |
| isolation and characterization of a carboxysome shell gene from thiobacillus neapolitanus. | the gene coding for the major carboxysome shell peptide (csos1) from thiobacillus neapolitanus has been isolated and sequenced. oligonucleotide primers for polymerase chain reaction (pcr) amplification of the 5' end of the gene were made possible by amino acid sequencing of the n-terminal residues of the shell peptide. a 41 bp pcr product was used as a probe to isolate the gene. the deduced amino acid composition of the 216 bp gene shows a high degree of hydrophobicity. the gene is located withi ... | 1994 | 7934888 |
| active site geometry in the high oxido-reduction potential rusticyanin from thiobacillus ferrooxidans. | rusticyanin is a blue copper protein involved in the oxidation of iron catalyzed by thiobacillus ferrooxidans. this protein is characterized by a high oxido-reduction potential and a high stability at low ph. the three dimensional structure of this protein is still unknown and in order to investigate the geometric properties of the copper center which could be correlated to the high oxido-reduction potential, we have studied rusticyanin by uv-visible, epr and nmr spectroscopies, at different ph ... | 1994 | 7945314 |
| biocorrosion produced by thiobacillus-like microorganisms. | biocorrosion can be produced by many different microorganisms through diverse mechanisms. the biocorrosion produced by acidophilic microorganisms of the genus thiobacillus is based on the production of sulfuric acid and ferric ion from pyrites or related mineral structures, as a result of the chemolithotrophic metabolism of these microorganisms. the products of this aerobic respiration are also powerful oxidant elements, which can produce chemical oxidations of other metallic structures. the tin ... | 1994 | 7946115 |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| the f1 genes of the f1f0 atp synthase from the acidophilic bacterium thiobacillus ferrooxidans complement escherichia coli f1 unc mutants. | an atp gene cluster from the extreme acidophile thiobacillus ferrooxidans was able to complement escherichia coli f1 unc mutants for growth on minimal medium plus succinate. complementation with all four e. coli f1 mutants tested was observed and subunits for the f1 portion of the t. ferrooxidans atp synthase formed a functional association with the f0 subunits of the e. coli enzyme. in addition, a hybrid f1 enzyme in which some units were derived from e. coli and some from t. ferrooxidans was p ... | 1994 | 7958772 |
| nuclear magnetic resonance 15n and 1h resonance assignments and global fold of rusticyanin. insights into the ligation and acid stability of the blue copper site. | nuclear magnetic resonance assignments are reported at ph approximately 3 for a type 1 ("blue") copper protein, rusticyanin, obtained from the acidophilic organism thiobacillus ferrooxidans. a combination of homonuclear proton and heteronuclear 15n-edited nmr spectra has been used to assign most of the 1h and 15n resonances of reduced rusticyanin. the copper-binding site is shown by analogy with other blue copper proteins to contain the side-chains of cys138, his143 and met148 at the c-terminal ... | 1994 | 7990128 |
| isolation of the thiobacillus ferrooxidans ntrbc genes using a t. ferrooxidans nifh-lacz fusion. | an agar plating technique was developed in which the activation of expression of a thiobacillus ferrooxidans nifh-lacz gene fusion was used to isolate the ntrbc genes from a t. ferrooxidans gene library. an escherichia coli ntrc mutant containing the nifh-lacz fusion was transformed and plated on a low-nitrogen medium so that on flooding with onpg, the production of yellow colonies indicated the presence of the cloned t. ferrooxidans ntrbc genes. a 4.47 kb region from the t. ferrooxidans chromos ... | 1994 | 8000526 |
| kinetics of the reduction of wild-type and mutant cytochrome c-550 by methylamine dehydrogenase and amicyanin from thiobacillus versutus. | to elucidate the kinetic properties of the methylamine dehydrogenase (madh) redox chain of thiobacillus versutus the reduction of cytochrome c-550 by madh and amicyanin has been studied. under steady state conditions, the rate constants of the reactions have been determined as a function of the ionic strength, both for wild type cytochrome c-550 and for mutants in which the conserved residue lys14 has been replaced as follows: lys14-->gln (mutant [k14q]cytochrome c-550) and lys14-->glu (mutant [ ... | 1994 | 8020493 |
| genetic organization of the mau gene cluster in methylobacterium extorquens am1: complete nucleotide sequence and generation and characteristics of mau mutants. | the nucleotide sequence of the methylamine utilization (mau) gene region from methylobacterium extorquens am1 was determined. open reading frames for 11 genes (maufbedacjglmn) were found, all transcribed in the same orientation. the maub, maua, and mauc genes encode the periplasmic methylamine dehydrogenase (madh) large and small subunit polypeptides and amicyanin, respectively. the products of maud, maug, maul, and maum were also predicted to be periplasmic. the products of mauf, maue, and maun ... | 1994 | 8021187 |
| solution structure of the type 1 blue copper protein amicyanin from thiobacillus versutus. | a three-dimensional solution structure of amicyanin from thiobacillus versutus has been determined by distance geometry and restrained molecular dynamics. a total of 984 experimentally derived constraints were used for the final refinement (881 distance constraints and 103 dihedral angle constraints). stereospecific assignments were made for 17 prochiral beta-methylene protons (33%) and the methyl groups of eight valine residues. fourteen structures were selected to represent the solution struct ... | 1994 | 8035459 |
| two membrane-bound c-type cytochromes of thiobacillus ferrooxidans: purification and properties. | membrane-bound cytochrome c, cytochrome c-552 (m) was purified from thiobacillus ferrooxidans. it showed an absorption peak at 410 nm in the oxidized form, and peaks at 552, 523 and 416 nm in the reduced form. its molecular mass, em,7 and isoelectric point were 22,300, +0.336 volt and 9.1, respectively. another membrane-bound cytochrome c, cytochrome c-550 (m) was also purified. it showed an absorption peak at 408 nm in the oxidized form, and peaks at 550, 523 and 418 nm in the reduced form. its ... | 1994 | 8039654 |
| characterization of is1201, an insertion sequence isolated from lactobacillus helveticus. | is1201, a 1387-bp insertion sequence isolated from lactobacillus helveticus, was identified by its nucleotide (nt) sequence. it carries a single open reading frame encoding a 369-amino-acid protein, which shares homology with transposases found in a class of related is, including isrm3 from rhizobium meliloti, is256 from staphylococcus aureus, is6120 from mycobacterium smegmatis, is1081 from m. bovis, ist2 from thiobacillus ferroxidans and is406 from pseudomonas cepacia. is1201 has terminal inve ... | 1994 | 8045427 |
| respiratory enzymes of thiobacillus ferrooxidans. kinetic properties of an acid-stable iron:rusticyanin oxidoreductase. | rusticyanin is an acid-stable, soluble blue copper protein found in abundance in the periplasmic space of thiobacillus ferrooxidans, an acidophilic bacterium capable of growing autotrophically on soluble ferrous sulfate. an acid-stable iron:rusticyanin oxidoreductase activity was partially purified from cell-free extracts of t. ferrooxidans. the enzyme-catalyzed, iron-dependent reduction of the rusticyanin exhibited three kinetic properties characteristic of aerobic iron oxidation by whole cells ... | 1994 | 8049223 |
| transposition of ist2 in thiobacillus ferrooxidans. | the genome of thiobacillus ferrooxidans contains at least two different repetitive dna elements. one of these elements, termed ist2 has been sequenced and shown to exhibit the characteristics of a typical prokaryotic insertion sequence. furthermore, preliminary evidence has implicated ist2 in genomic rearrangements, although the mechanism of rearrangement, whether by transposition or recombination, has not been established. in this report we provide evidence from detailed restriction enzyme anal ... | 1994 | 8057836 |
| characterization of mutant met100lys of cytochrome c-550 from thiobacillus versutus with lysine-histidine heme ligation. | the heme iron in cytochrome c-550 from thiobacillus versutus has a methionine and a histidine as axial ligands. in order to study the characteristics of a possible lysine-histidine ligation in a heme protein, the methionine has been replaced by a lysine. this residue acts as a ligand between ph 3 and 12. the midpoint potential of the mutant has shifted -329 mv compared to wild type, but apart from this shift the ph dependence of the midpoint potential is unchanged, suggesting that the large drop ... | 1994 | 8060974 |
| the role of lysine 99 of thiobacillus versutus cytochrome c-550 in the alkaline transition. | the methionine ligand of the heme iron in ferricytochrome c-550 from thiobacillus versutus is replaced by another residue at high ph. this transition is similar to the alkaline transition in mitochondrial cytochrome c. to investigate the possible role of lysine 99 in this process, this residue has been mutated to a glutamate. the mutation causes the apparent pka of the transition to decrease from 11.2 in wild type to 10.8 in lys99glu cytochrome c-550. this destabilization of the native form is a ... | 1994 | 8076674 |
| purification and characterization of two new c-type cytochromes involved in fe2+ oxidation from thiobacillus ferrooxidans. | two new c-type cytochromes have been purified from cell membranes of the acidophilic thiobacillus ferrooxidans. in contrast to a soluble cytochrome c with molecular mass of 14 kda reported earlier, a membrane-bound cytochrome c with a mass of 21 kda was solubilized with octylthioglucoside and purified to homogeneity. in addition, a high molecular mass c-type cytochrome (68 kda) was also solubilized and purified using triton x-100 as a detergent. both acid-stable species are partially released du ... | 1994 | 8082827 |
| crystal structure analysis and refinement at 2.15 a resolution of amicyanin, a type i blue copper protein, from thiobacillus versutus. | the crystal structure of the type i blue copper protein amicyanin from thiobacillus versutus has been determined by patterson search techniques on the basis of the molecular model of amicyanin from paracoccus denitrificans, and refined by energy-restrained least-squares methods. amicyanin crystallizes in the trigonal space group p3(2) with unit cell dimensions of a = b = 87.40 a, c = 38.20 a. the asymmetric unit is composed of three independent molecules centred on the crystallographic 3(2) axes ... | 1994 | 8120896 |
| transfer and expression of degradative and antibiotic resistance plasmids in acidophilic bacteria. | the genetic accessibility of selected acidophilic bacteria was investigated to evaluate their applicability to degrading pollutants in acidic environments. the incp1 antibiotic resistance plasmids rp4 and pvk101 and the phenol degradation-encoding plasmid ppgh11 were transferred from neutrophilic bacteria into the extreme acidophilic eubacterium acidiphilium cryptum at frequencies of 1.8 x 10(-2) to 9.8 x 10(-4) transconjugants per recipient cell. the incq antibiotic resistance plasmid psup106 w ... | 1994 | 8161188 |
| molecular genetics of thiobacillus ferrooxidans. | thiobacillus ferrooxidans is a gram-negative, highly acidophilic (ph 1.5 to 2.0), autotrophic bacterium that obtains its energy through the oxidation of ferrous iron or reduced inorganic sulfur compounds. it is usually dominant in the mixed bacterial populations that are used industrially for the extraction of metals such as copper and uranium from their ores. more recently, these bacterial consortia have been used for the biooxidation of refractory gold-bearing arsenopyrite ores prior to the re ... | 1994 | 8177170 |
| bacterial catalytic processes for transformation of metals. | microorganisms actively participate in the transformation of metals and metalloids by various processes including adsorption, absorption, alkylation, oxidation and reduction reactions. bacteria of the genera thiobacillus and sulfolobus have a chemolithotrophic mode of metabolism and catalyze various metal transformations. these bacteria are primarily involved in oxidation-reduction reactions of metals. metal sulfides can be either directly oxidized by the microbes or oxidized by ferric iron, an ... | 1993 | 8181951 |
| plasmid and transposon transfer to thiobacillus ferrooxidans. | the broad-host-range incp plasmids rp4, r68.45, rp1::tn501, and pub307 were transferred to acidophilic, obligately chemolithotrophic thiobacillus ferrooxidans from escherichia coli by conjugation. a genetic marker of kanamycin resistance was expressed in t. ferrooxidans. plasmid rp4 was transferred back to e. coli from t. ferrooxidans. the broad-host-range incq vector pjrd215 was mobilized to t. ferrooxidans with the aid of plasmid rp4 integrated in the chromosome of e. coli sm10. pjrd215 was st ... | 1994 | 8188590 |
| amino acid sequence of a high redox potential ferredoxin (hipip) from the purple phototrophic bacterium rhodopila globiformis, which has the highest known redox potential of its class. | rhodopila globiformis hipip has a redox potential (ca. 450 mv) that is 100 mv higher than any other known iron-sulfur protein. the amino acid sequence contains 57 residues and can be aligned with that of thiobacillus ferrooxidans without any insertions or deletions and is 51% identical. rp. globiformis hipip is also similar to that of rhodocyclus tenuis, but six- and two-residue gaps must be postulated and there is only 37% identity. most of the amino acid residues near the iron-sulfur cluster a ... | 1993 | 8215406 |
| parallel induction of nitric oxide and glucose-6-phosphate dehydrogenase in activated bone marrow derived macrophages. | the production of nitric oxide (no.) and the induction of glucose-6-phosphate dehydrogenase by lipopolysaccharides (lps) from different sources was studied in bone marrow derived macrophages (bmm phi). no. production was found to be linked to the induction of glucose-6-phosphate dehydrogenase, suggesting the possible involvement of this enzyme in the cytotoxic mechanism resulting from the release of no. by activated macrophages. | 1993 | 8216310 |
| 3-isopropylmalate dehydrogenase from chemolithoautotroph thiobacillus ferrooxidans: dna sequence, enzyme purification, and characterization. | 3-isopropylmalate dehydrogenase encoded by the thiobacillus ferrooxidans leub gene was purified to homogeneity from escherichia coli cells harboring a recombinant plasmid containing the leub gene. the native enzyme molecule is a dimer of molecular weight 38,000. the km value for 3-isopropylmalate was estimated to be 26 microm and that for nad+ 0.8 mm. the presence of k+ or nh4+ is essential for the enzyme reaction. the enzyme is activated about 4-fold by the addition of 1.0 mm mg2+ or co2+. the ... | 1993 | 8282728 |
| a new mechanism for the aerobic catabolism of dimethyl sulfide. | aerobic degradation of dimethyl sulfide (dms), previously described for thiobacilli and hyphomicrobia, involves catabolism to sulfide via methanethiol (ch3sh). methyl groups are sequentially eliminated as hcho by incorporation of o2 catalyzed by dms monooxygenase and methanethiol oxidase. h2o2 formed during ch3sh oxidation is destroyed by catalase. we recently isolated thiobacillus strain asn-1, which grows either aerobically or anaerobically with denitrification on dms. comparative experiments ... | 1993 | 8285684 |
| aerobic and anaerobic degradation of a range of alkyl sulfides by a denitrifying marine bacterium. | a pure culture of a bacterium was obtained from a marine microbial mat by using an anoxic medium containing dimethyl sulfide (dms) and nitrate. the isolate grew aerobically or anaerobically as a denitrifier on alkyl sulfides, including dms, dimethyl disulfide, diethyl sulfide (des), ethyl methyl sulfide, dipropyl sulfide, dibutyl sulfide, and dibutyl disulfide. cells grown on an alkyl sulfide or disulfide also oxidized the corresponding thiols, namely, methanethiol, ethanethiol, propanethiol, or ... | 1993 | 8285707 |
| effect of ph on sulfite oxidation by thiobacillus thiooxidans cells with sulfurous acid or sulfur dioxide as a possible substrate. | the oxidation of sulfite by thiobacillus thiooxidans was studied at various ph values with changing concentrations of potassium sulfite. the optimal ph for sulfite oxidation by cells was a function of sulfite concentrations, rising with increasing substrate concentrations, while that by the cell extracts was unaffected. the sulfite oxidation by cells was inhibited at high sulfite concentrations, particularly at low ph values. the results from kinetic studies show that the fully protonated form o ... | 1994 | 8300544 |
| amplification of ribulose biphosphate carboxylase/oxygenase large subunit (rubisco lsu) gene fragments from thiobacillus ferrooxidans and a moderate thermophile using polymerase chain reaction. | southern blot analysis of dna from an iron-oxidising moderate thermophile nmw-6 and from thiobacillus ferrooxidans strain tfi-35 demonstrated sequences homologous to the rubisco lsu gene of synechococcus. dna fragments (457 bp) encoding part of the rubisco lsu gene (amino acids 73-200) were amplified from the genomic dna of thiobacillus ferrooxidans and the moderate thermophile nmw-6 using the polymerase chain reaction (pcr) technique (saiki et al. (1985) science 233, 1350-1354). a comparison wi ... | 1993 | 8357616 |
| enzymes of aerobic respiration on iron. | bacteria capable of aerobic respiration on ferrous ions are spread throughout eubacterial and archaebacterial phyla. comparative spectroscopic analyses revealed that phylogenetically distinct organisms expressed copious quantities of spectrally distinct redox-active biomolecules during autotrophic growth on soluble iron. thiobacillus ferroxidans, leptospirillum ferrooxidans, sulfobacillus thermosulfidooxidans, and metallosphaera sedula possessed iron respiratory chains dominated by a blue copper ... | 1993 | 8357617 |
| [analysis of restriction samples of chromosomal dna from strains of thiobacillus ferrooxidans by pulse electrophoresis]. | 1993 | 8371723 | |
| cloning and sequencing of the gene coding for the large subunit of methylamine dehydrogenase from thiobacillus versutus. | the gene that codes for the alpha-subunit of methylamine dehydrogenase from thiobacillus versutus, mada, was cloned and sequenced. it codes for a protein of 395 amino acids preceded by a leader sequence of 31 amino acids. the derived amino acid sequence was confirmed by partial amino acid sequencing. the start of the mature protein could not be determined by direct sequencing, since the n terminus appeared to be blocked. instead, it was determined by electrospray mass spectrometry. confirmation ... | 1993 | 8407797 |
| specific binding of thiobacillus ferrooxidans rbcr to the intergenic sequence between the rbc operon and the rbcr gene. | the presence of two sets (rbcl1-rbcs1 and rbcl2-rbcs2) of rbc operons has been demonstrated in thiobacillus ferrooxidans fe1 (t. kusano, t. takeshima, c. inoue, and k. sugawara, j. bacteriol. 173:7313-7323, 1991). a possible regulatory gene, rbcr, 930 bp long and possibly translated into a 309-amino-acid protein, was found upstream from the rbcl1 gene as a single copy. the gene is located divergently to rbcl1 with a 144-bp intergenic sequence. as in the cases of the chromatium vinosum rbcr and a ... | 1993 | 8432695 |
| amino-acid sequence of rusticyanin from thiobacillus ferrooxidans and its comparison with other blue copper proteins. | rusticyanin, a copper protein characterized by a high redox potential (+680 mv) and a high stability at acidic ph, is involved in iron oxidation in thiobacillus ferrooxidans. it has been characterized from a new strain and its amino-acid sequence has been determined and compared to two other rusticyanin sequences isolated from different strains. it comprises 155 amino acids and the alignment of the three rusticyanins shows a high degree of homology. comparing the rusticyanins with six blue coppe ... | 1993 | 8448191 |
| cloning and expression of the d-ribulose-1,5-bisphosphate carboxylase/oxygenase form ii gene from thiobacillus intermedius in escherichia coli. | both form i and ii ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) genes were detected in thiobacillus intermedius by heterologous hybridization using specific probes from anacystis nidulans and rhodobacter sphaeroides, respectively. however, only the previously reported form i enzyme could be demonstrated in cells grown under a number of different conditions. the reason(s) why the form ii gene is not expressed in t. intermedius is/are not clear at this time. the form ii gene was isola ... | 1993 | 8472910 |
| phosphate-starvation induced changes in thiobacillus ferrooxidans. | we have analysed the response of the acidophilic chemolithotroph thiobacillus ferrooxidans to phosphate starvation. cultivation of the bacteria in the absence of added phosphate induced a remarkable filamentation of the cells. polyacrylamide gel electrophoresis revealed several proteins whose levels increased upon phosphate limitation, as well as some polypeptides that were exclusively synthesized under this growth limitation. one of the proteins whose level increased by the lack of phosphate wa ... | 1993 | 8472923 |
| transfer of thiosphaera pantotropha to paracoccus denitrificans. | comparative sequence analysis of in vitro-amplified 16s rrna genes of thiosphaera pantotropha gb17t (t = type strain) and paracoccus denitrificans lmg 4218t revealed identical 16s rrna primary structures for the two organisms. the level of overall dna similarity of thiosphaera pantotropha gb17t and p. denitrificans dsm 65t is 85%, as determined by quantitative dna-dna hybridization. therefore, we propose the transfer of thiosphaera pantotropha to p. denitrificans. the closest relative of thiosph ... | 1993 | 8494744 |
| x-ray studies of quinoproteins. | 1995 | 8524150 | |
| structure and function of tn5467, a tn21-like transposon located on the thiobacillus ferrooxidans broad-host-range plasmid ptf-fc2. | a 3.5-kb region of plasmid ptf-fc2, which contains a transposon-like element designated tn5467, has been sequenced, and its biological activity has been investigated. the transposon is bordered by two 38-bp inverted repeat sequences which have sequence identity in 37 of 38 and in 38 of 39 bp to the tnpa distal and tnpa proximal inverted repeats of tn21, respectively. within these borders, open reading frames with amino acid similarity to a glutaredoxin-like protein, a merr regulatory protein, an ... | 1995 | 8534089 |
| microbial ecology of simultaneous thermophilic microbial leaching and digestion of sewage sludge. | the microbial population encountered during a simultaneous thermophilic microbial leaching and digestion process at 50 degrees c, based on microbial sulfur oxidation, was investigated. the cell count of the sulfuric acid producer thiobacillus thermosulfatus increased, followed by a decrease. in the absence of sulfur (control: conventional thermophilic digestion), thiobacillus thermosulfatus population decreased under the detection limit. acidophilic and neutrophilic heterotrophic populations inc ... | 1995 | 8542551 |
| reduced sulfur compound oxidation by thiobacillus caldus. | the oxidation of reduced inorganic sulfur compounds was studied by using resting cells of the moderate thermophile thiobacillus caldus strain ku. the oxygen consumption rate and total oxygen consumed were determined for the reduced sulfur compounds thiosulfate, tetrathionate, sulfur, sulfide, and sulfite in the absence and in the presence of inhibitors and uncouplers. the uncouplers 2,4-dinitrophenol and carbonyl cyanide m-chlorophenyl-hydrazone had no affect on the oxidation of thiosulfate, sug ... | 1996 | 8550443 |
| deduced amino acid sequence, functional expression, and unique enzymatic properties of the form i and form ii ribulose bisphosphate carboxylase/oxygenase from the chemoautotrophic bacterium thiobacillus denitrificans. | the cbbl cbbs and cbbm genes of thiobacillus denitrificans, encoding form i and form ii ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), respectively, were found to complement a rubisco-negative mutant of rhodobacter sphaeroides to autotrophic growth. endogenous t. denitrificans promoters were shown to function in r. sphaeroides, resulting in high levels of cbbl cbbs and cbbm expression in the r. sphaeroides host. this expression system provided high levels of both t. denitrificans enz ... | 1996 | 8550452 |
| characterization of the ptfi91-family replicon of thiobacillus ferrooxidans plasmids. | plasmids found in six strains of thiobacillus ferrooxidans were mapped and compared in an effort to detect the origin of replication. four strains yielded an identical 9.8-kb plasmid, ptfi91. restriction mapping and southern blot hybridization analysis were used to confirm this finding. dissimilar plasmids found in two other strains contained a conserved 2.2-kb saci region common to ptfi91. dna sequence analysis of this region showed structural features common to bacterial plasmid replicons. a c ... | 1995 | 8590413 |
| analysis of the paramagnetic copper(ii) site of amicyanin by 1h nmr spectroscopy. | application of the tailored pulse sequences like super-weft allows the direct observation of the hyperfine-shifted signals of the paramagnetic cu(ii) forms of blue copper proteins in solution. the signals can be assigned by applying 2d nmr techniques, like exsy, to solutions containing a mixture of reduced and oxidized species. the fermi contact shift is separated from the pseudocontact shift on the basis of the known g-tensor anisotropy of the cu(ii) state, allowing the determination of a numbe ... | 1996 | 8608149 |
| characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from thiobacillus denitrificans. | cytochrome cd1-nitrite reductase and nitrous oxide reductase of thiobacillus denitrificans were purified and characterized by biochemical and immunochemical methods. in contrast to the generally soluble nature of the denitrification enzymes, these two enzymes were isolated from the membrane fraction of t. denitrificans and remained active after solubilization with triton x-100. the properties of the membrane-derived enzymes were similar to those of their soluble counterparts from the same organi ... | 1996 | 8639023 |
| [use of polymerase chain reaction for detecting the rbpc gene in natural samples]. | a method was developed allowing detection of the ribulose bisphosphate carboxylase (rubisco)-encoding gene in natural samples. the method is based on polymerase chain reaction (pcr) in the presence of oligonucleotide primers complementary to the conserved fragments of the rbcl gene from oxy- and anoxygenic phototrophic bacteria and some chemoautotrophic bacteria. use of this method made it possible to detect the rbcl gene in samples of natural water and sediments from antarctic lakes of the bung ... | 1995 | 8643038 |
| purification and characterization of the hydrogenase from thiobacillus ferrooxidans. | hydrogenase of thiobacillus ferrooxidans atcc 19859 was purified from cells grown lithoautotrophically with 80% hydrogen, 8.6% carbon dioxide, and 11.4% air. hydrogenase was located in the 140,000 x g supernatant in cell-free extracts. the enzyme was purified 7.3-fold after chromatography on procion red and q-sepharose with a yield of 19%, resulting in a 85% pure preparation with a specific activity of 6.0 u (mg protein)-1. with native page, a mol. mass of 100 and 200 kda was determined. with sd ... | 1996 | 8661919 |
| electrostatic environment of the tryptophylquinone cofactor in methylamine dehydrogenase: evidence from resonance raman spectroscopy of model compounds. | methylamine dehydrogenase (madh) utilizes its endogenous tryptophan tryptophylquinone (ttq) as a cofactor in enzymatic catalysis, with the c6 carbonyl of the quinone implicated as the site of attack by substrates and other nucleophiles. resonance raman (rr) spectroscopy provides an ideal method for investigating the state of this carbonyl group whose c==o stretch is distinct from other vibrational modes of the cofactor and is readily identified by its shift to lower energy in h218o. in a series ... | 1996 | 8664261 |
| redox reactivity of the type 1 copper protein amicyanin from thiobacillus versutus with its physiological partner cytochrome c550 and inter-protein cross-reaction studies. | reduction potentials eo' for the t. versutus amicyanin couple, amcuii/i, were determined at ph values in the range 4.4-9.0 by direct measurement using cyclic voltammetry, and from rate constants for the reactions amcu1 + [co(terpy)2]3+ and [co(terpy)2]2+ + amcuii, using an eo' for the [co(terpy)2]2+/3+ couple of 260 mv. at ph > 7.5 the value obtained is 236 mv, which increases with decreasing ph in keeping with proton inactivation of amcui. together with previously determined eo' values for the ... | 1996 | 8695651 |
| pcr-mediated detection of the chemolithotrophic bacterium thiobacillus cuprinus using 23s rdna- and 16s/23s intergenic spacer region-targeted oligonucleotide primers. | bioleaching is carried out by chemolithotrophic microorganisms, most of them belonging to the genera thiobacillus and leptospirillum. the role of the mixotrophic species t. cuprinus in this process is controversial, since its ecological study applying classical detection techniques to natural or industrial environments is very difficult. for this reason, we have developed an alternative method based on pcr-mediated detection using specific oligonucleotide primers that target variable regions of ... | 1996 | 8810512 |
| influence of preservation substrate on iron oxidation ability of various thiobacillus ferrooxidans isolates. | iron oxidation abilities of 11 isolates were compared after their preservation on ferrous sulphate and polymetallic sulfide concentrate containing 2.5 g l-1 cu and 45 g l-1 zn respectively in the leached solution. the fastest growing cultures, tf cd-2, grown on ferrous substrate showed the shortest iron oxidation period of 35 h as compared to 47 h by tf lig preserved on polymetallic concentrate for six months under ambient conditions, for > 95% iron oxidation. all the isolates except tf lig show ... | 1996 | 8817915 |
| sulfur-binding protein of flagella of thiobacillus ferrooxidans. | the sulfur-binding protein of thiobacillus ferrooxidans atcc 23270 was investigated. the protein composition of the bacterium's cell surface changed according to the culture substrate. sulfur-grown cells showed greater adhesion to sulfur than iron-grown cells. the sulfur-grown cells synthesized a 40-kda surface protein which was not synthesized by iron-grown cells. the 40-kda protein had thiol groups and strongly adhered to elemental sulfur powder. this adhesion was not disturbed by triton x-100 ... | 1996 | 8824625 |
| the gene for gamma-glutamylcysteine synthetase from thiobacillus ferrooxidans has low homology to its escherichia coli equivalent and is linked to the gene for citrate synthase. | the gene for gamma-glutamylcysteine synthetase (gsha) from thiobacillus ferrooxidans was isolated from a family of cosmids by its ability to complement an escherichia coli gsha trxa double mutant which was unable to grow on minimal medium lacking glutathione. the predicted sequence of the gamma-glutamylcysteine synthetase was found to have only 18% amino acid sequence identity to the equivalent enzyme from e. coli. in spite of this low sequence homology, concentrations of gsh in a cell extract p ... | 1996 | 8828222 |
| molecular aspects of the electron transfer system which participates in the oxidation of ferrous ion by thiobacillus ferrooxidans. | the enzymes and redox proteins, which participate in the oxidation of ferrous ion by the acidophilic iron-oxiding bacterium thiobacillus ferrooxidans, have been isolated and characterized. they are fe(ii)-cytochrome c oxidoreductase, cytochromes c-552(s), c-552(m) and c-550(m), rusticyanin, and cytochrome c oxidase. on the basis of the interactions of these components, an electron transfer system has been proposed which seems to function in the oxidation of ferrous ion by the bacterium. | 1995 | 8845189 |
| the influence of the peptide chain on the kinetics and stability of microperoxidases. | microperoxidases with increasing lengths of the peptide attached to the heme moiety have been isolated after proteolytic digestion of horse-heart cytochrome c (microperoxidases 6, 8, and 11) and of cytochrome c550 from thiobacillus versutus (microperoxidase 17). the different microperoxidases catalyze the h2o2-dependent para-hydroxylation of aniline relatively efficiently but are rapidly inactivated under turnover conditions. the horse-heart cytochrome-c-derived microperoxidases have identical v ... | 1996 | 8898909 |
| identification of membrane-bound c-type cytochromes in an acidophilic ferrous ion oxidizing bacterium thiobacillus ferrooxidans. | three membrane-bound acid-stable cytochromes c with molecular masses of 46, 30 and 21 kda were characterized from a new thiobacillus ferrooxidans strain. they were solubilized with high concentrations of dodecylmaltoside at ph 8. the 30 kda cytochrome c was purified to a homogeneous state as established by sds-page analysis. it showed an absorption peak at 410 nm in the oxidized form and at 418, 523 and 552 nm in the reduced form. the 46 kda cytochrome c co-purified with a non-heme protein of 36 ... | 1996 | 8919455 |
| bacterial populations in samples of bioleached copper ore as revealed by analysis of dna obtained before and after cultivation. | the composition of bacterial populations in copper bioleaching systems was investigated by analysis of dna obtained either directly from ores or leaching solutions or after laboratory cultures. this analysis consisted of the characterization of the spacer regions between the 16 and 23s genes in the bacterial rrna genetic loci after pcr amplification. the sizes of the spacer regions, amplified from dnas obtained from samples, were compared with the sizes of those obtained from cultures of the mai ... | 1996 | 8919792 |
| cloning and sequencing of the gene for the thiobacillus ferrooxidans atcc33020 glutamate synthase (gogat) small subunit and complementation of an escherichia coli gltd mutant. | a recombinant plasmid which contains the gltd gene coding for the glutamate synthase (gogat) small subunit was isolated from a thiobacillus ferrooxidans atcc33020 gene bank by complementation of an escherichia coli gltd mutant. the sequence of gltd was determined. the deduced amino acid sequence shows strong similarity to the two other prokaryote gltd sequences available, namely those of e. coli and a. brasilense (53% and 45% identity, respectively). a cosmid containing the gltbd region was isol ... | 1996 | 8921877 |
| isolation, phenotypic characterization, and phylogenetic position of a novel, facultatively autotrophic, moderately thermophilic bacterium, thiobacillus thermosulfatus sp. nov. | thiobacillus thermosulfatus atcc 51520t (t = type strain) was isolated from sewage sludge samples enriched with elemental sulfur. the cells of this organism were gram negative, rod shaped, motile, facultatively autotrophic, and strictly aerobic and contained polyphosphate inclusions and polyhedral bodies. during growth on thiosulfate, the following intermediates were produced: tetrathionate, trithionate, and sulfate, and the ph was lowered from neutrality to around 2.5. autotrophic growth was ob ... | 1996 | 8934899 |
| the structural gene for rusticyanin from thiobacillus ferrooxidans: cloning and sequencing of the rusticyanin gene. | the rusticyanin gene from the acidophilic chemolithotroph thiobacillus ferrooxidans has been cloned and sequenced. a central portion of the gene was identified by pcr reactions utilising primers optimised for codon bias followed by nested pcr with degenerate primers. the 5' and 3' ends of the rusticyanin gene were then cloned using degenerate primers to each end and anchor sequences to the known internal sequence. the entire gene was amplified using tli dna polymerase and specific primers to the ... | 1996 | 8935662 |
| multiple wavelength anomalous diffraction (mad) crystal structure of rusticyanin: a highly oxidizing cupredoxin with extreme acid stability. | the x-ray crystal structure of the oxidized form of the extremely stable and highly oxidizing cupredoxin rusticyanin from thiobacillus ferrooxidans has been determined by the method of multiwavelength anomalous diffraction (mad) and refined to 1.9 a resolution. like other cupredoxins, rusticyanin is a copper-containing metalloprotein, which is composed of a core beta-sandwich fold. in rusticyanin the beta-sandwich is composed of a six- and a seven-stranded beta-sheet. also like other cupredoxins ... | 1996 | 8947572 |
| nmr solution structure of cu(i) rusticyanin from thiobacillus ferrooxidans: structural basis for the extreme acid stability and redox potential. | the solution structure of the cu(i) form of the rusticyanin from thiobacillus ferrooxidans has been calculated from a total of 1979 distance and dihedral angle constraints derived from 1h, 13c and 15n nmr spectra. the structures reveal two beta-sheets, one of six strands and one of seven strands that are tightly packed in a beta-barrel or beta-sandwich arrangement, and a short helix that extends on the outside of one of the sheets to form a second hydrophobic core. the copper coordination sphere ... | 1996 | 8947573 |
| [metal resistance systems in pseudomonas]. | several chromosome- and plasmid-encoded metal resistance genetic systems have been studied in pseudomonas and related bacteria. some systems are known with molecular detail whereas others are still poorly understood. the former include resistance genes for cations derived from mercury, cadmium and copper and anions from arsenic and chromium. except for mercury, where a redox transformation occurs, extrusion of the toxic ions from the bacterial cytoplasm appears to be the most common mechanism of ... | 1996 | 8966398 |
| characterisation of a soluble cytochrome c4 isolated from thiobacillus ferrooxidans. | a soluble c-type cytochrome was purified to homogeneity from thiobacillus ferrooxidans. this cytochrome is characterised by an alpha-peak wavelength of 552 nm, a molecular mass of 21 193 da (as determined by mass spectroscopy), and a pi value of 9. n-terminal sequencing yielded the polypeptide sequence up to the 50th residue. the iron content of 1.9 fe/molecule and the heme/molecule ratio of 2.15 identified this cytochrome as a diheme protein. optical redox titrations at ph 3.0 revealed the pres ... | 1996 | 8973648 |
| nmr assignments and relaxation studies of thiobacillus versutus ferrocytochrome c-550 indicate the presence of a highly mobile 13-residues long c-terminal tail. | cytochrome c-550 of thiobacillus versutus functions as an electron transfer protein in a chain of redox proteins that enables t. versutus to grow on methylamine. it is a single-heme protein of 134 residues, related to mitochondrial cytochrome c. cytochrome c-550, as well as several other bacterial c2-type cytochromes, contain a c-terminal extension of 13-16 amino acids of unknown function, compared to mitochondrial cytochrome c. nmr experiments were performed to obtain structural and dynamic inf ... | 1996 | 8976558 |
| mercuric ion uptake by escherichia coli cells producing thiobacillus ferrooxidans merc. | the merc gene of thiobacillus ferrooxidans was overexpressed in escherichia coli under the control of the tac promoter. merc protein synthesized in e. coli has a n-terminal amino acid sequence of s-a-i-x-r-i-i-d-k-i-g-i-v-g-, which agrees with the amino acid sequence deduced from its nucleotide sequence except that an initiating methionine residue was removed. the merc protein was localized in the particulate (membrane) cell fraction, and not in the soluble cytoplasmic fraction. e. coli cells ca ... | 1996 | 8987545 |