Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| the citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic escherichia coli. | citrobacter rodentium (formally citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. in common with enteropathogenic and enterohemorrhagic escherichia coli (epec and ehec, respectively), c. rodentium exploits a type iii secretion system (t3ss) to induce attaching and effacing (a/e) lesions that are essential for virulence. here, we report the fully annotated genome sequence of the 5.3-mb chromosome and four plasmids ... | 2010 | 19897651 |
| the citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic escherichia coli. | citrobacter rodentium (formally citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. in common with enteropathogenic and enterohemorrhagic escherichia coli (epec and ehec, respectively), c. rodentium exploits a type iii secretion system (t3ss) to induce attaching and effacing (a/e) lesions that are essential for virulence. here, we report the fully annotated genome sequence of the 5.3-mb chromosome and four plasmids ... | 2010 | 19897651 |
| rapid detection of aac(6')-ib-cr quinolone resistance gene by pyrosequencing. | pyrosequencing was used to rapidly detect aac(6')-ib and aac(6')-ib-cr genes. this plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing enterobacteriaceae. this method is faster and more cost-effective than the methods previously described. sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing. | 2009 | 19923484 |
| rapid detection of aac(6')-ib-cr quinolone resistance gene by pyrosequencing. | pyrosequencing was used to rapidly detect aac(6')-ib and aac(6')-ib-cr genes. this plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing enterobacteriaceae. this method is faster and more cost-effective than the methods previously described. sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing. | 2009 | 19923484 |
| isolation and molecular characterization of multidrug-resistant gram-negative bacteria from imported flamingos in japan. | imported animals, especially those from developing countries, may constitute a potential hazard to native animals and to public health. in this study, a new flock of lesser flamingos imported from tanzania to hiroshima zoological park were screened for multidrug-resistant gram-negative bacteria, integrons and antimicrobial resistance genes. thirty-seven gram-negative bacterial isolates were obtained from the flamingos. seven isolates (18.9%) showed multidrug resistance phenotypes, the most commo ... | 2009 | 19930691 |
| direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. | with long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (maldi-tof) mass spectrometry was demonstrated to accurately identify bacteria routinely isolated in a clinical biology laboratory. in order to speed up the identification proce ... | 2009 | 19946369 |
| rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry is a rapid, accurate method for identifying bacteria and fungi recovered on agar culture media. we report herein a method for the direct identification of bacteria in positive blood culture broths by maldi-tof mass spectrometry. a total of 212 positive cultures were examined, representing 32 genera and 60 species or groups. the identification of bacterial isolates by maldi-tof mass spectrometry was compared ... | 2010 | 19955282 |
| rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry is a rapid, accurate method for identifying bacteria and fungi recovered on agar culture media. we report herein a method for the direct identification of bacteria in positive blood culture broths by maldi-tof mass spectrometry. a total of 212 positive cultures were examined, representing 32 genera and 60 species or groups. the identification of bacterial isolates by maldi-tof mass spectrometry was compared ... | 2010 | 19955282 |
| antimicrobial resistance among nosocomial isolates in a teaching hospital in goa. | emergence of polyantimicrobial resistant strains of hospital pathogens has presented a challenge in the provision of good quality in-patient care. inappropriate use of antibiotics in the hospital is largely responsible for this catastrophe. bacteriological surveillance of the cases of nosocomial infections is crucial for framing an evidence-based antimicrobial policy for a hospital. | 2008 | 19967031 |
| actinobaculum schaalii, a common uropathogen in elderly patients, denmark. | actinobaculum schaalii can cause urinary tract infections and septicemia but is difficult to identify by cultivation. to obtain a fast diagnosis and identify a. schaalii, we developed a taqman real-time quantitative pcr. routine urine samples were obtained from 177 hospitalized patients and 75 outpatients in viborg county, denmark, in 2008-2009. the pcr detected a. schaalii in 22% of samples from patients >60 years of age. this assay showed that a. schaalii is more common than implied by routine ... | 2010 | 20031046 |
| feedback inhibition in the phoq/phop signaling system by a membrane peptide. | the phoq/phop signaling system responds to low magnesium and the presence of certain cationic antimicrobial peptides. it regulates genes important for growth under these conditions, as well as additional genes important for virulence in many gram-negative pathogens. phoq is a sensor kinase that phosphorylates and activates the transcription factor phop. since feedback inhibition is a common theme in stress-response circuits, we hypothesized that some members of the phop regulon may play such a r ... | 2009 | 20041203 |
| [citrobacter koseri brain abscess in an infant: case report and literature review]. | we present a 2 month-old female infant admitted to the hospital with macrocephaly as main clinical sign, irritability, and vomiting. the computer tomography scanning showed a cystic frontal image with contralateral ventricle displacement. surgical intervention was performed. purulent material culture was positive for citrobacter koseri (diversus). cerebrospinal fluid culture was negative. the infant was treated with antibiotics for 6 weeks with a good outcome. she presented postsurgical hydrocep ... | 2009 | 20049403 |
| high-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular identification systems in a conventional microbiological laboratory. first, we evaluated maldi-tof ms using 327 clinical isolates previously cultured from patient materials and identified by conventional techniques (vitek-ii, api, and biochemical tests). discre ... | 2010 | 20053859 |
| three decades of beta-lactamase inhibitors. | since the introduction of penicillin, beta-lactam antibiotics have been the antimicrobial agents of choice. unfortunately, the efficacy of these life-saving antibiotics is significantly threatened by bacterial beta-lactamases. beta-lactamases are now responsible for resistance to penicillins, extended-spectrum cephalosporins, monobactams, and carbapenems. in order to overcome beta-lactamase-mediated resistance, beta-lactamase inhibitors (clavulanate, sulbactam, and tazobactam) were introduced in ... | 2010 | 20065329 |
| reprogramming of anaerobic metabolism by the fnrs small rna. | small rnas (srnas) that act by base pairing with trans-encoded mrnas modulate metabolism in response to a variety of environmental stimuli. here, we describe an hfq-binding srna (fnrs) whose expression is induced upon a shift from aerobic to anaerobic conditions and which acts to downregulate the levels of a variety of mrnas encoding metabolic enzymes. anaerobic induction in minimal medium depends strongly on fnr but is also affected by the arca and crp transcription regulators. whole genome exp ... | 2010 | 20070527 |
| evolution in quantum leaps: multiple combinatorial transfers of hpi and other genetic modules in enterobacteriaceae. | horizontal gene transfer is a key step in the evolution of enterobacteriaceae. by acquiring virulence determinants of foreign origin, commensals can evolve into pathogens. in enterobacteriaceae, horizontal transfer of these virulence determinants is largely dependent on transfer by plasmids, phages, genomic islands (gis) and genomic modules (gms). the high pathogenicity island (hpi) is a gi encoding virulence genes that can be transferred between different enterobacteriaceae. we investigated the ... | 2010 | 20084283 |
| identification and characterization of repetitive extragenic palindromes (rep)-associated tyrosine transposases: implications for rep evolution and dynamics in bacterial genomes. | bacterial repetitive extragenic palindromes (reps) compose a distinct group of genomic repeats. they usually occur in high abundance (>100 copies/genome) and are often arranged in composite repetitive structures - bacterial interspersed mosaic elements (bimes). in bimes, regularly spaced reps are present in alternating orientations. bimes and reps have been shown to serve as binding sites for several proteins and suggested to play role in chromosome organization and transcription termination. th ... | 2010 | 20085626 |
| complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative escherichia coli strain 042. | escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. several studies suggest enteroaggregative e. coli are the predominant cause of e. coli-mediated diarrhea in the developed world and are second only to campylobacter sp. as a cause of bacterial-mediated diarrhea. furthermore, enteroaggregative e. coli are a predominant cause of persistent diarrhea in the developing world where infection h ... | 2010 | 20098708 |
| citrobacter koseri cellulitis during anti-cd20 monoclonal antibody (ofatumumab) treatment for b-cell chronic lymphocytic leukaemia. | 2010 | 20107742 | |
| ctx-m-35 extended-spectrum beta-lactamase conferring ceftazidime resistance in citrobacter koseri. | 2010 | 20138478 | |
| diffuse retinal vasculitis in citrobacter koseri traumatic endophthalmitis with intraocular foreign body. | 2010 | 20154565 | |
| abundance of type i toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families. | small, hydrophobic proteins whose synthesis is repressed by small rnas (srnas), denoted type i toxin-antitoxin modules, were first discovered on plasmids where they regulate plasmid stability, but were subsequently found on a few bacterial chromosomes. we used exhaustive psi-blast and tblastn searches across 774 bacterial genomes to identify homologs of known type i toxins. these searches substantially expanded the collection of predicted type i toxins, revealed homology of the ldr and fst toxin ... | 2010 | 20156992 |
| sequence analysis and characterization of a transferable hybrid plasmid encoding multidrug resistance and enabling zoonotic potential for extraintestinal escherichia coli. | colv plasmids of extraintestinal pathogenic escherichia coli (expec) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. these plasmids are found significantly more often in expec, including expec associated with human neonatal meningitis and avian colibacillosis, than in commensal e. coli. here we describe papec-o103-colbm, a hybrid repfiia/fib plasmid harboring components of the colv pathogenicity island and a multidrug resi ... | 2010 | 20160015 |
| maternal plasma retinol binding protein 4 in acute pyelonephritis during pregnancy. | adipokines have been implicated in metabolic regulation and the immune response thus providing a molecular mechanism for the interaction between these two systems. retinol binding protein 4 (rbp4) is a novel adipokine that plays a role in the pathophysiology of obesity-induced insulin resistance, as well as in the modulation of inflammation. the aim of this study was to determine whether there are changes in maternal plasma concentrations of rbp4 in pregnant women with acute pyelonephritis. | 2010 | 20163326 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets. | an ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (maldi-tof) mass spectrometry analysis. identification was obtained for 78.7% of the pellets tested. moreover, 99% of the maldi-tof identifications were congruent at the species level when considering valid scores. this fast and accurate method is promising. | 2010 | 20164269 |
| comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. | bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species identification. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is an emerging technology newly applied to the problem of bacterial species identification. we evaluated two maldi-tof ms systems with 720 consecutively isolated bacterial colonies under routine clinical laboratory conditions. isolates were analyzed ... | 2010 | 20164271 |
| microbial keratitis secondary to infection with citrobacter koseri. | 2010 | 20164753 | |
| shewanella spp. genomic evolution for a cold marine lifestyle and in-situ explosive biodegradation. | shewanella halifaxensis and shewanella sediminis were among a few aquatic gamma-proteobacteria that were psychrophiles and the first anaerobic bacteria that degraded hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx). although many mesophilic or psychrophilic strains of shewanella and gamma-proteobacteria were sequenced for their genomes, the genomic evolution pathways for temperature adaptation were poorly understood. on the other hand, the genes responsible for anaerobic rdx mineralization pathways ... | 2010 | 20174598 |
| hyperresistinemia - a novel feature in systemic infection during human pregnancy. | resistin, originally described as an adipokine, has emerged as a potent pro-inflammatory protein associated with both acute and chronic inflammation. moreover, resistin has been proposed as a powerful marker of sepsis severity, as well as a predictor of survival of critically ill non-pregnant patients. the aim of this study was to determine whether pyelonephritis during pregnancy is associated with changes in maternal plasma resistin concentrations. | 2010 | 20178460 |
| extended-spectrum-beta-lactamase, ampc, and carbapenemase issues. | 2010 | 20181902 | |
| fatal citrobacter farmeri meningitis in a patient with nasopharyngeal cancer. | we describe the case of an adult male with nasopharyngeal carcinoma who had meningitis caused by citrobacter farmeri. the isolate was confirmed as c. farmeri by two commercial identification systems and 16s rrna gene analysis. the patient developed multiorgan failure and died despite antibiotic treatment with in vitro active agents (ceftriaxone and meropenem). | 2010 | 20181904 |
| controlling false-positive results obtained with the hodge and masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic acid. | the modified hodge method (mht) has been recommended by the clsi for confirmation of suspected class a carbapenemase production in species of enterobacteriaceae. this test and the masuda method (mas) have advantages over traditional phenotypic methods in that they directly analyze carbapenemase activity. in order to identify the potential interferences of these tests, we designed a panel composed of diverse bacterial genera with distinct carbapenem susceptibility patterns (42 carbapenemase produ ... | 2010 | 20181912 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |
| acute pyelonephritis during pregnancy changes the balance of angiogenic and anti-angiogenic factors in maternal plasma. | angiogenic factors have been implicated in the pathophysiology of sepsis. in experimental models of sepsis (endotoxemia and/or cecal ligation puncture), there is increased expression of vascular endothelial growth factors (vegf) and the administration of exogenous soluble vegf receptor (svegfr)-1, an antagonist to vegf, reduces morbidity and mortality. moreover, a dramatic elevation in svegfr-1 has been demonstrated in human sepsis. although a balance between angiogenic and anti-angiogenic facto ... | 2010 | 20213923 |
| fragment bb: evidence for activation of the alternative pathway of the complement system in pregnant women with acute pyelonephritis. | pyelonephritis during pregnancy is associated with a more severe course than in the non-pregnant state. this has been attributed to an increased susceptibility of pregnant women to microbial products. the complement system is part of innate immunity and its alternative pathway is activated mainly by microorganisms. the purpose of this study was to determine if activation of the alternative pathway of the complement system (determined by maternal fragment bb concentrations) occurs in pregnant wom ... | 2010 | 20218820 |
| performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has recently been introduced in diagnostic microbiology laboratories for the identification of bacterial and yeast strains isolated from clinical samples. in the present study, we prospectively compared maldi-tof ms to the conventional phenotypic method for the identification of routine isolates. colonies were analyzed by maldi-tof ms either by direct deposition on the target plate or after a formic acid- ... | 2010 | 20220166 |
| citrobacter spp. simultaneously harboring blactx-m, blatem, blashv, blaampc, and insertion sequences is26 and orf513: an evolutionary phenomenon of recent concern for antibiotic resistance. | a collection of 40 confirmed isolates of citrobacter spp., obtained from the culture of 5,732 clinical samples during 2006 to 2008, was studied for the presence of blactx-m, blatem, blashv, and blaampc by monoplex and multiplex pcrs. these isolates were also looked at for the presence of insertion elements is26 and orf513, which play an important role in the resistance gene pool. blactx-m, blatem, blashv, and blaampc were noticed in 67.5%, 40%, 25%, and 40% of isolates, respectively. sequencing ... | 2010 | 20220171 |
| genome sequence of cronobacter sakazakii baa-894 and comparative genomic hybridization analysis with other cronobacter species. | the genus cronobacter (formerly called enterobacter sakazakii) is composed of five species; c. sakazakii, c. malonaticus, c. turicensis, c. muytjensii, and c. dublinensis. the genus includes opportunistic human pathogens, and the first three species have been associated with neonatal infections. the most severe diseases are caused in neonates and include fatal necrotizing enterocolitis and meningitis. the genetic basis of the diversity within the genus is unknown, and few virulence traits have b ... | 2010 | 20221447 |
| agrobacterium tumefaciens type iv secretion protein virb3 is an inner membrane protein and requires virb4, virb7, and virb8 for stabilization. | agrobacterium tumefaciens virb proteins assemble a type iv secretion apparatus and a t-pilus for secretion of dna and proteins into plant cells. the pilin-like protein virb3, a membrane protein of unknown topology, is required for the assembly of the t-pilus and for t-dna secretion. using phoa and green fluorescent protein (gfp) as periplasmic and cytoplasmic reporters, respectively, we demonstrate that virb3 contains two membrane-spanning domains and that both the n and c termini of the protein ... | 2010 | 20348257 |
| pharmacokinetic-pharmacodynamic-model-guided doripenem dosing in critically ill patients. | the growing number of infections caused by multidrug-resistant pathogens has prompted a more rational use of available antibiotics given the paucity of new, effective agents. monte carlo simulations were utilized to determine the appropriateness of several doripenem dosing regimens based on the probability of attaining the critical drug exposure metric of time that drug concentrations remain above the drug mic (t>mic) for 35% (and lower thresholds) of the dosing interval in >80 to 90% of the pop ... | 2010 | 20385857 |
| direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has been suggested as a reliable method for bacterial identification from cultures. direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. we compared conventional methods for the diagnosis of urinary tract infections (utis) and identification of the urinary tract pathogens (automated screening, plate cultures, and identifica ... | 2010 | 20392910 |
| spectrum and prevalence of fungi infecting deep tissues of lower-limb wounds in patients with type 2 diabetes. | the prevalence rate and spectrum of fungi infecting deep tissues of diabetic lower-limb wounds (dlws) have not been previously studied. five hundred eighteen (382 male and 136 female) consecutive patients with type 2 diabetes hospitalized due to infected lower-limb wounds were enlisted in this study. deep tissue (approximately 0.5- x 0.5-cm size) taken perioperatively from the wound bed was cultured for fungi. fungi was found in 27.2% (141/518) of the study population. candida parapsilosis (25.5 ... | 2010 | 20410345 |
| fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of salmonella spp. in a broad spectrum of samples. | a fluorescence in situ hybridization (fish) method for the rapid detection of salmonella spp. using a novel peptide nucleic acid (pna) probe was developed. the probe theoretical specificity and sensitivity were both 100%. the pna-fish method was optimized, and laboratory testing on representative strains from the salmonella genus subspecies and several related bacterial species confirmed the predicted theoretical values of specificity and sensitivity. the pna-fish method has been successfully ad ... | 2010 | 20453122 |
| genome sequence of the plant growth promoting endophytic bacterium enterobacter sp. 638. | enterobacter sp. 638 is an endophytic plant growth promoting gamma-proteobacterium that was isolated from the stem of poplar (populus trichocarpaxdeltoides cv. h11-11), a potentially important biofuel feed stock plant. the enterobacter sp. 638 genome sequence reveals the presence of a 4,518,712 bp chromosome and a 157,749 bp plasmid (pent638-1). genome annotation and comparative genomics allowed the identification of an extended set of genes specific to the plant niche adaptation of this bacteri ... | 2010 | 20485560 |
| antimicrobial resistant bacteria among health care workers in intensive care units at ain shams university hospitals. | fifty hcws in icus of internal medicine, chest, neonatology and burn were included in prospective cohort study. collection of nasal, hand and rectal swabs, proper biochemical identification, culture media and antibiotic sensitivity tests were used to detect methicillin-resistant staphylococcus aureus (mrsa); vancomycin-resistant enterococci (vre) & extended spectrum beta-lactamase producing gram -ve bacilli (esbls). s. aureus was isolated from 34% of hcws; 28% were nasal carriers, 4% were hand c ... | 2010 | 20503587 |
| phylogenetic incongruence arising from fragmented speciation in enteric bacteria. | evolutionary relationships among species are often assumed to be fundamentally unambiguous, where genes within a genome are thought to evolve in concert and phylogenetic incongruence between individual orthologs is attributed to idiosyncrasies in their evolution. we have identified substantial incongruence between the phylogenies of orthologous genes in escherichia, salmonella, and citrobacter, or e. coli, e. fergusonii, and e. albertii. the source of incongruence was inferred to be recombinatio ... | 2010 | 20534528 |
| evaluation of a dna microarray, the check-points esbl/kpc array, for rapid detection of tem, shv, and ctx-m extended-spectrum beta-lactamases and kpc carbapenemases. | extended-spectrum beta-lactamases (esbls) and klebsiella pneumoniae carbapenemases (kpc carbepenemases) have rapidly emerged worldwide and require rapid identification. the check-points esbl/kpc array, a new commercial system based on genetic profiling for the direct identification of esbl producers (shv, tem, and ctx-m) and of kpc producers, was evaluated. well-characterized gram-negative rods (enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii) expressing various ss-lactamases ... | 2010 | 20547813 |
| chromosomally encoded blacmy-2 located on a novel sxt/r391-related integrating conjugative element in a proteus mirabilis clinical isolate. | integrating conjugative elements (ices) are mobile genetic elements that can transfer from the chromosome of a host to the chromosome of a new host through the process of excision, conjugation, and integration. although sxt/r391-related ices, originally demonstrated in vibrio cholerae o139 isolates, have become prevalent among v. cholerae isolates in asia, the prevalence of the ices among gram-negative bacteria other than vibrio spp. remains unknown. in addition, sxt/r391-related ices carrying g ... | 2010 | 20566768 |
| molecular analysis of type 3 fimbrial genes from escherichia coli, klebsiella and citrobacter species. | catheter-associated urinary tract infection (cauti) is the most common nosocomial infection in the united states and is caused by a range of uropathogens. biofilm formation by uropathogens that cause cauti is often mediated by cell surface structures such as fimbriae. in this study, we characterised the genes encoding type 3 fimbriae from cauti strains of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter koseri and citrobacter freundii. | 2010 | 20576143 |
| rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr. | pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. the present study was aimed to develop a novel quantitative pcr (qpcr) assay, using ecfx as the specific target gene, for the rapid and accurate identification of p. aeruginosa from positive blood cultures (bcs). | 2010 | 20684778 |
| microglia dynamics and function in the cns. | microglial cells constitute the resident immune cell population of the mammalian central nervous system. one striking feature of these cells is their highly dynamic nature under both normal and pathological brain conditions. the highly branched processes of resting microglia display a constitutive mobility and undergo rapid directional movement towards sites of acute tissue disruption. microglia can be converted by a large number of different stimuli to a chronically activated state by signaling ... | 2010 | 20705452 |
| a bacterial mrna leader that employs different mechanisms to sense disparate intracellular signals. | bacterial mrnas often contain leader sequences that respond to specific metabolites or ions by altering expression of the associated downstream protein-coding sequences. here we report that the leader rna of the mg(2+) transporter gene mgta of salmonella enterica, which was previously known to function as a mg(2+)-sensing riboswitch, harbors an 18 codon proline-rich open reading frame-termed mgtl-that permits intracellular proline to regulate mgta expression. interfering with mgtl translation by ... | 2010 | 20813261 |
| suppression subtractive hybridization identifies an autotransporter adhesin gene of e. coli imt5155 specifically associated with avian pathogenic escherichia coli (apec). | extraintestinal pathogenic e. coli (expec) represent a phylogenetically diverse group of bacteria which are implicated in a large range of infections in humans and animals. although subgroups of different expec pathotypes, including uropathogenic, newborn meningitis causing, and avian pathogenic e. coli (apec) share a number of virulence features, there still might be factors specifically contributing to the pathogenesis of a certain subset of strains or a distinct pathotype. thus, we made use o ... | 2010 | 20828376 |
| aminoglycoside modifying enzymes. | aminoglycosides have been an essential component of the armamentarium in the treatment of life-threatening infections. unfortunately, their efficacy has been reduced by the surge and dissemination of resistance. in some cases the levels of resistance reached the point that rendered them virtually useless. among many known mechanisms of resistance to aminoglycosides, enzymatic modification is the most prevalent in the clinical setting. aminoglycoside modifying enzymes catalyze the modification at ... | 2010 | 20833577 |
| proteomic characterization of vana-containing enterococcus recovered from seagulls at the berlengas natural reserve, w portugal. | abstract: | 2010 | 20858227 |
| the prediction and characterization of ysha, an unknown outer-membrane protein from salmonella typhimurium. | we have developed an effective pathway for the prediction and characterization of novel transmembrane β-barrel proteins. the freeman-wimley algorithm, which is a highly accurate prediction method based on the physicochemical properties of experimentally characterized transmembrane β barrel (tmbb) structures, was used to predict tmbbs in the genome of salmonella typhimurium lt2. the previously uncharacterized product of gene ysha was tested as a model for validating the algorithm. ysha is a highl ... | 2010 | 20863811 |
| the prediction and characterization of ysha, an unknown outer-membrane protein from salmonella typhimurium. | we have developed an effective pathway for the prediction and characterization of novel transmembrane β-barrel proteins. the freeman-wimley algorithm, which is a highly accurate prediction method based on the physicochemical properties of experimentally characterized transmembrane β barrel (tmbb) structures, was used to predict tmbbs in the genome of salmonella typhimurium lt2. the previously uncharacterized product of gene ysha was tested as a model for validating the algorithm. ysha is a highl ... | 2010 | 20863811 |
| citrobacter koseri meningitis: another freediving risk? | we present a rare case of meningitis caused by citrobacter koseri in an immunocompetent adult who had recently been freediving. middle ear pressure changes from this recreational activity, and the subsequent inflammatory response, are likely to have provided this environmental organism access to the central nervous system, and thus the ability to cause clinically significant infection. | 2010 | 20933000 |
| mode-of-action studies of the novel bisquaternary bisnaphthalimide mt02 against staphylococcus aureus. | screening of various bisquaternary bisnaphthalimides against a variety of human pathogens revealed one compound, designated mt02, with strong inhibitory effects against gram-positive bacteria. the mics ranged from 0.31 μg/ml against community-acquired methicillin-resistant staphylococcus aureus (mrsa) lineage usa300 to 20 μg/ml against streptococcus pneumoniae. radioactive whole-cell labeling experiments indicated a strong impact of mt02 on bacterial dna replication. dna microarray studies gener ... | 2010 | 20937782 |
| mode-of-action studies of the novel bisquaternary bisnaphthalimide mt02 against staphylococcus aureus. | screening of various bisquaternary bisnaphthalimides against a variety of human pathogens revealed one compound, designated mt02, with strong inhibitory effects against gram-positive bacteria. the mics ranged from 0.31 μg/ml against community-acquired methicillin-resistant staphylococcus aureus (mrsa) lineage usa300 to 20 μg/ml against streptococcus pneumoniae. radioactive whole-cell labeling experiments indicated a strong impact of mt02 on bacterial dna replication. dna microarray studies gener ... | 2010 | 20937782 |
| gut inflammation in chronic fatigue syndrome. | chronic fatigue syndrome (cfs) is a debilitating disease characterized by unexplained disabling fatigue and a combination of accompanying symptoms the pathology of which is incompletely understood. many cfs patients complain of gut dysfunction. in fact, patients with cfs are more likely to report a previous diagnosis of irritable bowel syndrome (ibs), a common functional disorder of the gut, and experience ibs-related symptoms. recently, evidence for interactions between the intestinal microbiot ... | 2010 | 20939923 |
| severe imported falciparum malaria: a cohort study in 400 critically ill adults. | large studies on severe imported malaria in non-endemic industrialized countries are lacking. we sought to describe the clinical spectrum of severe imported malaria in french adults and to identify risk factors for mortality at admission to the intensive care unit. | 2010 | 20949045 |
| etiology and antibacterial susceptibility pattern of community-acquired bacterial ocular infections in a tertiary eye care hospital in south india. | to identify the etiology, incidence and prevalence of ocular bacterial infections, and to assess the in vitro susceptibility of these ocular bacterial isolates to commonly used antibiotics. | 2010 | 20952834 |
| molecular probes for diagnosis of clinically relevant bacterial infections in blood cultures. | broad-range real-time pcr and sequencing of the 16s rrna gene region is a widely known method for the detection and identification of bacteria in clinical samples. however, because of the need for sequencing, such identification of bacteria is time-consuming. the aim of our study was to develop a more rapid 16s real-time pcr-based identification assay using species- or genus-specific probes. the gram-negative bacteria were divided into pseudomonas species, pseudomonas aeruginosa, escherichia col ... | 2010 | 20962139 |
| distribution analysis of hydrogenases in surface waters of marine and freshwater environments. | surface waters of aquatic environments have been shown to both evolve and consume hydrogen and the ocean is estimated to be the principal natural source. in some marine habitats, h(2) evolution and uptake are clearly due to biological activity, while contributions of abiotic sources must be considered in others. until now the only known biological process involved in h(2) metabolism in marine environments is nitrogen fixation. | 2010 | 21079771 |
| physiological and evolutionary studies of nap systems in shewanella piezotolerans wp3. | most of the shewanella species contain two periplasmic nitrate reductases (nap-α and nap-β), which is a unique feature of this genus. in the present study, the physiological function and evolutionary relationship of the two nap systems were studied in the deep-sea bacterium shewanella piezotolerans wp3. both of the wp3 nap gene clusters: nap-α (napd1a1b1c) and nap-β (napd2a2b2) were shown to be involved in nitrate respiration. phylogenetic analyses suggest that nap-β originated earlier than nap- ... | 2010 | 21124486 |
| physiological and evolutionary studies of nap systems in shewanella piezotolerans wp3. | most of the shewanella species contain two periplasmic nitrate reductases (nap-α and nap-β), which is a unique feature of this genus. in the present study, the physiological function and evolutionary relationship of the two nap systems were studied in the deep-sea bacterium shewanella piezotolerans wp3. both of the wp3 nap gene clusters: nap-α (napd1a1b1c) and nap-β (napd2a2b2) were shown to be involved in nitrate respiration. phylogenetic analyses suggest that nap-β originated earlier than nap- ... | 2010 | 21124486 |
| can we use imipenem and meropenem vitek 2 mics for detection of suspected kpc and other-carbapenemase producers among species of enterobacteriaceae? | imipenem and meropenem vitek 2 mics were evaluated for a panel of 104 enterobacteriaceae for identification of carbapenemase producers. the sensitivity and specificity values for the new clsi interpretative criteria (clsi document m100-s20-u, 2010) were 98% and 83% for imipenem and 76% and 83% for meropenem, respectively. we propose an algorithm that is highly sensitive (98%) and specific (94%) for carbapenemase screening based on the combined use of imipenem and meropenem mics. | 2011 | 21159944 |
| citrobacter koseri as a cause of early periprosthetic infection after primary total hip arthroplasty. | periprosthetic joint infection in the acute setting is usually caused by gram-positive species and remains a major problem facing total joint surgeons. we report a case of a 53-year-old male who presented with drainage 3 weeks after primary total hip arthroplasty. citrobacter koseri was cultured from an infected hematoma in his deep tissues. surgical treatment included irrigation and debridement with femoral head and liner exchange. he received a 6-week course of ertapenem and is currently asymp ... | 2010 | 21167674 |
| a novel cdsab operon is involved in the uptake of l-cysteine and participates in the pathogenesis of yersinia ruckeri. | application of in vivo expression technology (ivet) to yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. analysis of the translational products of both genes showed permease domains (open reading frame 1 [orf1]) and amino acid position identities (orf2) with the l-cysteine desulfidase from methanocaldococcus jannaschii, a new type of enzyme involved in the br ... | 2010 | 21169490 |
| a novel cdsab operon is involved in the uptake of l-cysteine and participates in the pathogenesis of yersinia ruckeri. | application of in vivo expression technology (ivet) to yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. analysis of the translational products of both genes showed permease domains (open reading frame 1 [orf1]) and amino acid position identities (orf2) with the l-cysteine desulfidase from methanocaldococcus jannaschii, a new type of enzyme involved in the br ... | 2010 | 21169490 |
| effective treatment of folliculitis decalvans using selected antimicrobial agents. | folliculitis decalvans (fd) is a rare neutrophilic infammation of the scalp characterized by painful, recurrent purulent follicular exudation resulting in primary cicatricial alopecia. however, unclear etiology makes fd treatment a difficult task. a wide variety of topical and systemic agents have been tried previously, with varied results. we present here a case series report of a set of 13 patients with fd on antimicrobial therapy. | 2010 | 21188019 |
| strain-resolved community genomic analysis of gut microbial colonization in a premature infant. | the intestinal microbiome is a critical determinant of human health. alterations in its composition have been correlated with chronic disorders, such as obesity and inflammatory bowel disease in adults, and may be associated with neonatal necrotizing enterocolitis in premature infants. increasing evidence suggests that strain-level genomic variation may underpin distinct ecological trajectories within mixed populations, yet there have been few strain-resolved analyses of genotype-phenotype conne ... | 2010 | 21191099 |
| strain-resolved community genomic analysis of gut microbial colonization in a premature infant. | the intestinal microbiome is a critical determinant of human health. alterations in its composition have been correlated with chronic disorders, such as obesity and inflammatory bowel disease in adults, and may be associated with neonatal necrotizing enterocolitis in premature infants. increasing evidence suggests that strain-level genomic variation may underpin distinct ecological trajectories within mixed populations, yet there have been few strain-resolved analyses of genotype-phenotype conne ... | 2010 | 21191099 |
| comparison of bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to bd phoenix automated microbiology system for identification of gram-negative bacilli. | we compared the bd phoenix automated microbiology system to the bruker biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant results. the bd phoenix correctly identified 363 (83%) and 330 (75%) isolates to the genus and species level, respectively. the bruker biotyper correctly identified 408 (93%) and 360 (82 ... | 2011 | 21209160 |
| the aspartate-semialdehyde dehydrogenase of edwardsiella ictaluri and its use as balanced-lethal system in fish vaccinology. | asda mutants of gram-negative bacteria have an obligate requirement for diaminopimelic acid (dap), which is an essential constituent of the peptidoglycan layer of the cell wall of these organisms. in environments deprived of dap, i.e., animal tissues, they will undergo lysis. deletion of the asda gene has previously been exploited to develop antibiotic-sensitive strains of live attenuated recombinant bacterial vaccines. introduction of an asd(+) plasmid into a δasda mutant makes the bacterial st ... | 2010 | 21209920 |
| comprehensive approaches to molecular biomarker discovery for detection and identification of cronobacter spp. (enterobacter sakazakii) and salmonella spp. | cronobacter spp. (formerly enterobacter sakazakii) and salmonella spp. are increasingly implicated internationally as important microbiological contaminants in low-moisture food products, including powdered infant formula. estimates indicate that 40 to 80% of infants infected with cronobacter sakazakii and/or salmonella in the united states may not survive the illness. a systematic approach, combining literature-based data mining, comparative genome analysis, and the direct sequencing of pcr pro ... | 2011 | 21239552 |
| comparison of nine phenotypic methods for detection of extended-spectrum beta-lactamase production by enterobacteriaceae. | the detection of extended-spectrum β-lactamase-producing (esbl) bacteria is of importance for infection control and epidemiological surveillance. we aimed to compare phenotypic methods available in the routine laboratory and to evaluate two-step strategies using these methods for the detection of esbl-positive enterobacteriaceae. two methods used for routine susceptibility testing (vitek2 and disk diffusion methods) and seven methods designed for the detection of esbl production (esbl etests, co ... | 2011 | 21248086 |
| natural products genomics. | 2008 | 21261848 | |
| mechanism of action of and resistance to quinolones. | fluoroquinolones are an important class of wide-spectrum antibacterial agents. the first quinolone described was nalidixic acid, which showed a narrow spectrum of activity. the evolution of quinolones to more potent molecules was based on changes at positions 1, 6, 7 and 8 of the chemical structure of nalidixic acid. quinolones inhibit dna gyrase and topoisomerase iv activities, two enzymes essential for bacteria viability. the acquisition of quinolone resistance is frequently related to (i) chr ... | 2008 | 21261881 |
| mechanism of action of and resistance to quinolones. | fluoroquinolones are an important class of wide-spectrum antibacterial agents. the first quinolone described was nalidixic acid, which showed a narrow spectrum of activity. the evolution of quinolones to more potent molecules was based on changes at positions 1, 6, 7 and 8 of the chemical structure of nalidixic acid. quinolones inhibit dna gyrase and topoisomerase iv activities, two enzymes essential for bacteria viability. the acquisition of quinolone resistance is frequently related to (i) chr ... | 2008 | 21261881 |
| directed networks reveal genomic barriers and dna repair bypasses to lateral gene transfer among prokaryotes. | lateral gene transfer (lgt) plays a major role in prokaryote evolution with only a few genes that are resistant to it; yet the nature and magnitude of barriers to lateral transfer are still debated. here, we implement directed networks to investigate donor-recipient events of recent lateral gene transfer among 657 sequenced prokaryote genomes. for 2,129,548 genes investigated, we detected 446,854 recent lateral gene transfer events through nucleotide pattern analysis. among these, donor-recipien ... | 2011 | 21270172 |
| rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis. | we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ... | 2011 | 21289149 |
| a udp-hexnac:polyprenol-p galnac-1-p transferase (wecp) representing a new subgroup of the enzyme family. | the aeromonas hydrophila ah-3 wecp represents a new class of udp-hexnac:polyprenol-p hexnac-1-p transferases. these enzymes use a membrane-associated polyprenol phosphate acceptor (undecaprenyl phosphate [und-p]) and a cytoplasmic udp-d-n-acetylhexosamine sugar nucleotide as the donor substrate. until now, all the weca enzymes tested were able to transfer udp-glcnac to the und-p. in this study, we present in vitro and in vivo proofs that a. hydrophila ah-3 wecp transfers galnac to und-p and is u ... | 2011 | 21335454 |
| aminoglycoside resistance rates, phenotypes, and mechanisms of gram-negative bacteria from infected patients in upper egypt. | with the re-emergence of older antibiotics as valuable choices for treatment of serious infections, we studied the aminoglycoside resistance of gram-negative bacteria isolated from patients with ear, urinary tract, skin, and gastrointestinal tract infections at minia university hospital in egypt. escherichia coli (mainly from urinary tract and gastrointestinal tract infections) was the most prevalent isolate (28.57%), followed by pseudomonas aeruginosa (25.7%) (mainly from ear discharge and skin ... | 2011 | 21359143 |
| functional characterization of novel mutations in ul54 of ganciclovir resistant hcmv strain using structural analysis. | this study reports the probable impact of the coupled mutations observed in our clinical isolate of hcmv ul54 polymerase, through structural bioinformatics approaches. the reported variant was found to be resistant to ganciclovir (gcv) as per the clinical records. the presence of glutamine deletion at 639 (glu639) and a mis sense mutation of serine 655 leucine (ser655leu) in ul54 were identified by dna sequencing and were predicted to lie in the dna polymerase type-ii domain. docking simulation ... | 2011 | 21383907 |
| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| characterization of the salmonella enterica serovar typhimurium ydci gene, which encodes a conserved dna binding protein required for full acid stress resistance. | salmonella enterica serovar typhimurium possesses a stimulon of genes that are differentially regulated in response to conditions of low fluid shear force that increase bacterial virulence and alter other phenotypes. in this study, we show that a previously uncharacterized member of this stimulon, ydci or stm1625, encodes a highly conserved dna binding protein with related homologs present in a range of gram-negative bacterial genera. gene expression analysis shows that ydci is expressed in diff ... | 2011 | 21398541 |
| highly specific and quick detection of mycobacterium avium subsp. paratuberculosis in feces and gut tissue of cattle and humans by multiple real-time pcr assays. | mycobacterium avium subsp. paratuberculosis is the causative agent of johne's disease (jd) in cattle and may be associated with crohn's disease (cd) in humans. it is the slowest growing of the cultivable mycobacteria, and culture from clinical, veterinary, food, or environmental specimens can take 4 months or even longer. currently, the insertion element is900 is used to detect m. avium subsp. paratuberculosis dna. however, closely related is900 elements are also present in other mycobacteria, t ... | 2011 | 21430100 |
| pyogenic liver abscess: an audit of 10 years' experience. | to describe our own experience with pyogenic liver abscesses over the past 10 years and investigate the risk factors associated with failure of initial percutaneous therapy. | 2011 | 21472130 |
| microbiology of animal bite wound infections. | the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ... | 2011 | 21482724 |
| myd88 is pivotal for immune recognition of citrobacter koseri and astrocyte activation during cns infection†. | abstract: citrobacter koseri (c. koseri) is a gram-negative bacterium that can cause a highly aggressive form of neonatal meningitis, which often progresses to establish multi-focal brain abscesses. the roles of toll-like receptor 4 (tlr4) and its signaling adaptor myd88 during cns c. koseri infection have not yet been examined, which is important since recent evidence indicates that innate immune responses are tailored towards specific pathogen classes. here tlr4 wt (c3h/fej) and tlr4 mutant (c ... | 2011 | 21496301 |
| genetic regulation of the rama locus and its expression in clinical isolates of klebsiella pneumoniae. | tigecycline resistance has been attributed to rama overexpression and subsequent acra upregulation. the rama locus, originally identified in klebsiella pneumoniae, has homologues in enterobacter and salmonella spp. in this study, we identify in silico that the ramr binding site is also present in citrobacter spp. and that enterobacter, citrobacter and klebsiella spp. share key regulatory elements in the control of the roma-rama locus. race (rapid amplification of cdna ends) mapping indicated tha ... | 2011 | 21514798 |
| the novel gene ygaw (alae) encodes an inducible l-alanine exporter in escherichia coli. | we previously isolated a mutant hypersensitive to l-alanyl-l-alanine from an l-alanine non-metabolizing escherichia coli strain and found that it lacked an inducible l-alanine export system. consequently, this mutant showed a significant accumulation of intracellular l-alanine and a reduction in the l-alanine export rate compared to the parent strain. when the mutant was used as a host to clone a gene(s) that complements the dipeptide-hypersensitive phenotype, two uncharacterized genes, ygaw and ... | 2011 | 21531828 |
| antimicrobial resistance in urinary tract pathogens in canada from 2007 to 2009: canward surveillance study. | from january 2007 to december 2009 an annual canadian national surveillance study (canward) tested 2943 urinary culture pathogens for antimicrobial susceptibilities following clinical and laboratory standards institute (clsi) guidelines. the most frequently isolated urinary pathogens (n, % of all isolates) were escherichia coli (1581, 54%), enterococci (410, 14%), klebsiella pneumoniae (274, 9%), proteus mirabilis (122, 4%), pseudomonas aeruginosa (100, 3%), and staphylococcus aureus (80, 3%). r ... | 2011 | 21537027 |
| monitoring procalcitonin in febrile neutropenia: what is its utility for initial diagnosis of infection and reassessment in persistent fever? | management of febrile neutropenic episodes (fe) is challenged by lacking microbiological and clinical documentation of infection. we aimed at evaluating the utility of monitoring blood procalcitonin (pct) in fe for initial diagnosis of infection and reassessment in persistent fever. | 2011 | 21541027 |
| rapid detection of blakpc carbapenamase genes by internally controlled real-time pcr assay from bactec(r) blood culture bottles. | rapid detection of drug resistant bacteria in clinical samples plays an instrumental role in patients' infection management and in implementing effective infection control policies. in this report, we validated a multiplex taqman real-time pcr (q-pcr) assay for the detection of blakpc genes and the human gene rnase p, in bactec® blood culture bottles. the magna pure lc 2.0, was utilized to extract nucleic acids from the inoculated broth while bovine serum albumin (bsa) was utilized as pcr inhibi ... | 2011 | 21562110 |
| bacterial syntenies: an exact approach with gene quorum. | the automatic identification of syntenies across multiple species is a key step in comparative genomics that helps biologists shed light both on evolutionary and functional problems. | 2011 | 21605461 |
| comparative genomic analysis of the hexuronate metabolism genes and their regulation in gamma-proteobacteria. | the hexuronate metabolism in escherichia coli is regulated by two related transcription factors from the fadr subfamily of the gntr family, uxur and exur. uxur controls the d-glucuronate metabolism, while exur represses genes involved in the metabolism of all hexuronates. here we use the comparative genomics approach to reconstruct the hexuronate metabolic pathways and transcriptional regulons in the gamma-proteobacteria. we demonstrate differences in the binding motifs of uxur and exur, identif ... | 2011 | 21622752 |