Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa by south florida plant extracts. | quorum sensing (qs) is a key regulator of virulence and biofilm formation in pseudomonas aeruginosa and other medically relevant bacteria. aqueous extracts of six plants, conocarpus erectus, chamaesyce hypericifolia, callistemon viminalis, bucida buceras, tetrazygia bicolor, and quercus virginiana, were examined in this study for their effects on p. aeruginosa virulence factors and the qs system. c. erectus, b. buceras, and c. viminalis caused a significant inhibition of lasa protease, lasb elas ... | 2008 | 17938186 |
| inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa by south florida plant extracts. | quorum sensing (qs) is a key regulator of virulence and biofilm formation in pseudomonas aeruginosa and other medically relevant bacteria. aqueous extracts of six plants, conocarpus erectus, chamaesyce hypericifolia, callistemon viminalis, bucida buceras, tetrazygia bicolor, and quercus virginiana, were examined in this study for their effects on p. aeruginosa virulence factors and the qs system. c. erectus, b. buceras, and c. viminalis caused a significant inhibition of lasa protease, lasb elas ... | 2008 | 17938186 |
| luxri homologs are universally present in the genus aeromonas. | aeromonas spp. have been regarded as "emerging pathogens". aeromonads possess multifactorial virulence and the production of many of these virulence determinants is associated with high cell density, a phenomenon that might be regulated by quorum sensing. however, only two species of the genus are reported to possess the luxri quorum sensing gene homologs. the purpose of this study was to investigate if the luxri homologs are universally present in the aeromonas strains collected from various cu ... | 2007 | 17953777 |
| signature-tagged mutagenesis of edwardsiella ictaluri identifies virulence-related genes, including a salmonella pathogenicity island 2 class of type iii secretion systems. | edwardsiella ictaluri is the leading cause of mortality in channel catfish culture, but little is known about its pathogenesis. the use of signature-tagged mutagenesis in a waterborne infection model resulted in the identification of 50 mutants that were unable to infect/survive in catfish. nineteen had minitransposon insertions in miscellaneous genes in the chromosome, 10 were in genes that matched to hypothetical proteins, and 13 were in genes that had no significant matches in the ncbi databa ... | 2007 | 17965213 |
| isolation and characterization of two groups of novel marine bacteria producing violacein. | thirteen strains of novel marine bacteria producing a purple pigment were isolated from the pacific coast of japan. they were divided into two groups based on their 16s ribosomal rna gene sequences, and both groups of bacteria belonged to the genus pseudoalteromonas. the uv-visible spectrum of the pigment was identical to those of violacein, a pigment produced by several species of bacteria including chromobacterium violaceum, an opportunistic pathogen. further analysis of the chemical structure ... | 2008 | 17968625 |
| the nlee/ospz family of effector proteins is required for polymorphonuclear transepithelial migration, a characteristic shared by enteropathogenic escherichia coli and shigella flexneri infections. | enteropathogenic escherichia coli (epec) and shigella flexneri are human host-specific pathogens that infect intestinal epithelial cells. however, each bacterial species employs a different infection strategy within this environmental niche. epec attaches to the apical surface of small intestine enterocytes, causing microvillus effacement and rearrangement of the host cell cytoskeleton beneath adherent bacteria. in contrast, s. flexneri invades the large intestine epithelium at the basolateral m ... | 2008 | 17984206 |
| the nlee/ospz family of effector proteins is required for polymorphonuclear transepithelial migration, a characteristic shared by enteropathogenic escherichia coli and shigella flexneri infections. | enteropathogenic escherichia coli (epec) and shigella flexneri are human host-specific pathogens that infect intestinal epithelial cells. however, each bacterial species employs a different infection strategy within this environmental niche. epec attaches to the apical surface of small intestine enterocytes, causing microvillus effacement and rearrangement of the host cell cytoskeleton beneath adherent bacteria. in contrast, s. flexneri invades the large intestine epithelium at the basolateral m ... | 2008 | 17984206 |
| [sepsis caused by pigmented and no pigmented chromobacterium violaceum]. | chromobacterium violaceum sepsis is rare but associated with a high mortality rate. we report a fatal case of c. violaceum sepsis in a 6 years old venezuelan indian boy. clinical manifestations were fever and swelling in the right inguinal region. the initial diagnosis was an appendicular plastron. appendicectomy was performed and during surgery a right psoas abscess was identified that resulted culture positive for pigmented c. violaceum. blood cultures were positive for a pigmented and non pig ... | 2007 | 17989847 |
| massetolide a biosynthesis in pseudomonas fluorescens. | massetolide a is a cyclic lipopeptide (clp) antibiotic produced by various pseudomonas strains from diverse environments. cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide a biosynthesis in p. fluorescens ss101 is governed by three nonribosomal peptide synthetase (nrps) genes, designated massa, massb, and massc, spanning approximately 30 kb. prediction of the nature and configuration of the amino acids by in silico analysis of adenylation and condensatio ... | 2008 | 17993540 |
| massetolide a biosynthesis in pseudomonas fluorescens. | massetolide a is a cyclic lipopeptide (clp) antibiotic produced by various pseudomonas strains from diverse environments. cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide a biosynthesis in p. fluorescens ss101 is governed by three nonribosomal peptide synthetase (nrps) genes, designated massa, massb, and massc, spanning approximately 30 kb. prediction of the nature and configuration of the amino acids by in silico analysis of adenylation and condensatio ... | 2008 | 17993540 |
| the [fefe] hydrogenase of nyctotherus ovalis has a chimeric origin. | the hydrogenosomes of the anaerobic ciliate nyctotherus ovalis show how mitochondria can evolve into hydrogenosomes because they possess a mitochondrial genome and parts of an electron-transport chain on the one hand, and a hydrogenase on the other hand. the hydrogenase permits direct reoxidation of nadh because it consists of a [fefe] hydrogenase module that is fused to two modules, which are homologous to the 24 kda and the 51 kda subunits of a mitochondrial complex i. | 2007 | 18021395 |
| identification of prophages in bacterial genomes by dinucleotide relative abundance difference. | prophages are integrated viral forms in bacterial genomes that have been found to contribute to interstrain genetic variability. many virulence-associated genes are reported to be prophage encoded. present computational methods to detect prophages are either by identifying possible essential proteins such as integrases or by an extension of this technique, which involves identifying a region containing proteins similar to those occurring in prophages. these methods suffer due to the problem of l ... | 2007 | 18030328 |
| comparison of genomes of three xanthomonas oryzae bacteriophages. | xp10 and op1 are phages of xanthomonas oryzae pv. oryzae (xoo), the causative agent of bacterial leaf blight in rice plants, which were isolated in 1967 in taiwan and in 1954 in japan, respectively. we recently isolated the xoo phage xop411. | 2007 | 18045507 |
| the guanine-nucleotide-exchange factor bope from burkholderia pseudomallei adopts a compact version of the salmonella sope/sope2 fold and undergoes a closed-to-open conformational change upon interaction with cdc42. | bope is a type iii secreted protein from burkholderia pseudomallei, the aetiological agent of melioidosis, a severe emerging infection. bope is a gef (guanine-nucleotide-exchange factor) for the rho gtpases cdc42 (cell division cycle 42) and rac1. we have determined the structure of bope catalytic domain (amino acids 78-261) by nmr spectroscopy and it shows that bope(78-261) comprises two three-helix bundles (alpha1alpha4alpha5 and alpha2alpha3alpha6). this fold is similar to that adopted by the ... | 2008 | 18052936 |
| evolution of the chaperone/usher assembly pathway: fimbrial classification goes greek. | many proteobacteria use the chaperone/usher pathway to assemble proteinaceous filaments on the bacterial surface. these filaments can curl into fimbrial or nonfimbrial surface structures (e.g., a capsule or spore coat). this article reviews the phylogeny of operons belonging to the chaperone/usher assembly class to explore the utility of establishing a scheme for subdividing them into clades of phylogenetically related gene clusters. based on usher amino acid sequence comparisons, our analysis s ... | 2007 | 18063717 |
| fatal septicemia caused by chromobacterium violaceum in a child from colombia. | a 4-year old child living in colombia presented with a history of fever and severe abdominal pain for four days. the patient developed pneumonia, septic shock, multiple organ failure and died on the fifth day of hospitalization. chromobacterium violaceum was isolated from admission blood cultures and was resistant to ampicillin, cephalosporins, carbapenems and aminoglycosides. | 2007 | 18157408 |
| the violacein biosynthetic enzyme vioe shares a fold with lipoprotein transporter proteins. | vioe, an unusual enzyme with no characterized homologues, plays a key role in the biosynthesis of violacein, a purple pigment with antibacterial and cytotoxic properties. without bound cofactors or metals, vioe, from the bacterium chromobacterium violaceum, mediates a 1,2 shift of an indole ring and oxidative chemistry to generate prodeoxyviolacein, a precursor to violacein. our 1.21 a resolution structure of vioe shows that the enzyme shares a core fold previously described for lipoprotein tran ... | 2008 | 18171675 |
| n-acylhomoserine lactone regulates violacein production in chromobacterium violaceum type strain atcc 12472. | in tests, chromobacterium violaceum atcc 12472 produced several n-acyl-l-homoserine lactones (ahls). of these, n-(3-hydroxydecanoyl)-l-homoserine lactone was dominant, and controlled violacein production by quorum sensing. strain vir07, an ahl-deficient mutant, did not produce violacein. violacein production in vir07 was induced by adding long-chain ahls (c10-c16), but was inhibited by adding short-chain ahls (c4-c8). strain vir07 showed the response of violacein production when ahls diffused fr ... | 2008 | 18177311 |
| the chaperone ipgc copurifies with the virulence regulator mxie. | the expression of a subset of shigella flexneri virulence genes is dependent upon a cytoplasmic chaperone, ipgc, and an activator from the arac/xyls family, mxie. in this paper, we report that the chaperone forms a specific and stable heteromer with mxie. | 2008 | 18192384 |
| a rhodococcus qsda-encoded enzyme defines a novel class of large-spectrum quorum-quenching lactonases. | a gene involved in n-acyl homoserine lactone (n-ahsl) degradation was identified by screening a genomic library of rhodococcus erythropolis strain w2. this gene, named qsda (for quorum-sensing signal degradation), encodes an n-ahsl lactonase unrelated to the two previously characterized n-ahsl-degrading enzymes, i.e., the lactonase aiia and the amidohydrolase aiid. qsda is related to phosphotriesterases and constitutes the reference of a novel class of n-ahsl degradation enzymes. it confers the ... | 2008 | 18192419 |
| fatal septicaemia due to chromobacterium violaceum. | human infection caused by chromobacterium violaceum is rare but when it occurs, it is associated with a high mortality rate. this is a report of a young adult male who presented as a surgical emergency and succumbed soon after. the most common feature of this infection is sepsis, followed by cutaneous involvement and liver abscesses. chromobacterium infection as a differential in a case of sepsis is important for clinicians to suspect, especially in tropical countries. | 2007 | 18198747 |
| novel microarray design strategy to study complex bacterial communities. | assessing bacterial flora composition appears to be of increasing importance to fields as diverse as physiology, development, medicine, epidemiology, the environment, and the food industry. we report here the development and validation of an original microarray strategy that allows analysis of the phylogenic composition of complex bacterial mixtures. the microarray contains approximately 9,500 feature elements targeting 16s rrna gene-specific regions. probe design was performed by selecting olig ... | 2008 | 18203854 |
| improved total synthesis of the potent hdac inhibitor fk228 (fr-901228). | a scaleable synthesis of the potent histone deacetylase (hdac) inhibitor fk228 is described. a reliable strategy for preparing the key beta-hydroxy mercapto heptenoic acid partner was accomplished in nine steps and 13% overall yield. a noyori asymmetric hydrogen-transfer reaction established the hydroxyl stereochemistry in >99:1 er via the reduction of a propargylic ketone. | 2008 | 18205373 |
| diversity and quorum-sensing signal production of proteobacteria associated with marine sponges. | marine sponges are hosts to diverse and dense bacterial communities and thus provide a potential environment for quorum sensing. quorum sensing, a key factor in cell-cell communication and bacterial colonization of higher animals, might be involved in the symbiotic interactions between bacteria and their sponge hosts. given that marine proteobacteria are known to produce n-acyl homoserine lactone (ahl) signal molecules, we tested the production of ahls by alpha- and gammaproteobacteria isolated ... | 2008 | 18211268 |
| structural basis for mannose recognition by a lectin from opportunistic bacteria burkholderia cenocepacia. | chronic colonization of the lungs by opportunist bacteria such as pseudomonas aeruginosa and members of the bcc (burkholderia cepacia complex) is the major cause of morbidity and mortality among cf (cystic fibrosis) patients. pa-iil (lecb gene), a soluble lectin from ps. aeruginosa, has been the subject of much interest because of its very strong affinity for fucose. orthologues have been identified in the opportunist bacteria ralstonia solanacearum, chromobacterium violaceum and burkholderia of ... | 2008 | 18215132 |
| residues near the amino terminus of rns are essential for positive autoregulation and dna binding. | most members of the arac/xyls family contain a conserved carboxy-terminal dna binding domain and a less conserved amino-terminal domain involved in binding small-molecule effectors and dimerization. however, there is no evidence that rns, a regulator of enterotoxigenic escherichia coli virulence genes, responds to an effector ligand, and in this study we found that the amino-terminal domain of rns does not form homodimers in vivo. exposure of rns to the chemical cross-linker glutaraldehyde revea ... | 2008 | 18223083 |
| control of acetic acid fermentation by quorum sensing via n-acylhomoserine lactones in gluconacetobacter intermedius. | a number of gram-negative bacteria regulate gene expression in a cell density-dependent manner by quorum sensing via n-acylhomoserine lactones (ahls). gluconacetobacter intermedius nci1051, a gram-negative acetic acid bacterium, produces three different ahls, n-decanoyl-l-homoserine lactone, n-dodecanoyl-l-homoserine lactone, and an n-dodecanoyl-l-homoserine lactone with a single unsaturated bond in its acyl chain, as determined by liquid chromatography-tandem mass spectrometry. two genes encodi ... | 2008 | 18245283 |
| higher infection of dengue virus serotype 2 in human monocytes of patients with g6pd deficiency. | the prevalence of glucose-6-phosphate dehydrogenase (g6pd) deficiency is high in asia. an ex vivo study was conducted to elucidate the association of g6pd deficiency and dengue virus (denv) infection when many asian countries are hyper-endemic. human monocytes from peripheral mononuclear cells collected from 12 g6pd-deficient patients and 24 age-matched controls were infected with one of two denv serotype 2 (denv-2) strains-the new guinea c strain (from a case of dengue fever) or the 16681 strai ... | 2008 | 18270558 |
| transcriptional regulation of nad metabolism in bacteria: nrtr family of nudix-related regulators. | a novel family of transcription factors responsible for regulation of various aspects of nad synthesis in a broad range of bacteria was identified by comparative genomics approach. regulators of this family (here termed nrtr for nudix-related transcriptional regulators), currently annotated as adp-ribose pyrophosphatases from the nudix family, are composed of an n-terminal nudix-like effector domain and a c-terminal dna-binding hth-like domain. nrtr regulons were reconstructed in diverse bacteri ... | 2008 | 18276643 |
| isolation and characterization of an autoinducer synthase from acinetobacter baumannii. | the opportunistic human pathogen acinetobacter baumannii strain m2 was found to produce distinct acyl-homoserine lactone (ahl) signals based on the use of an agrobacterium tumefaciens trag-lacz biosensor. an a. baumannii gene, designated abai, was cloned and directed ahl production in recombinant escherichia coli. the abai protein was similar to members of the luxi family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by a. baumannii. the primary ahl signa ... | 2008 | 18281398 |
| [successful treatment of a case with fatal sepsis caused by chromobacterium violaceum]. | 2007 | 18282432 | |
| cohesion group approach for evolutionary analysis of tyra, a protein family with wide-ranging substrate specificities. | many enzymes and other proteins are difficult subjects for bioinformatic analysis because they exhibit variant catalytic, structural, regulatory, and fusion mode features within a protein family whose sequences are not highly conserved. however, such features reflect dynamic and interesting scenarios of evolutionary importance. the value of experimental data obtained from individual organisms is instantly magnified to the extent that given features of the experimental organism can be projected u ... | 2008 | 18322033 |
| determination of the cyclic depsipeptide fk228 in human and mouse plasma by liquid chromatography with mass-spectrometric detection. | an analytical method was developed and validated for the quantitative determination of the cyclic depsipeptide fk228 (romidepsin, formerly fr901228; nsc 630176), a histone deacetylase inhibitor, in human and mouse plasma. calibration curves were linear in the concentration range of 2-1000 ng/ml. sample pretreatment involved a liquid-liquid extraction of 0.1 ml aliquots of plasma with ethyl acetate. fk228 and the internal standard, harmine, were separated on a zorbax sb c18 column (75 mm x 2.1mm, ... | 2008 | 18342585 |
| diversity in antifungal activity of strains of chromobacterium violaceum from the brazilian amazon. | chromobacterium violaceum is a free-living gram-negative bacterium found in soil and aquatic habitats; abundantly present in the brazilian amazon, it is an important example of exploitable microbial diversity of the tropics. in this study, 24 strains from the brazilian amazon and atcc 12472(t) were investigated for biocontrol potential of seven fungi pathogenic to soybean [glycine max (l.) merril] seed. both cells and the supernatants of two brazilian strains, 07-1 and 27-1, together with atcc 1 ... | 2008 | 18347828 |
| crystal structure of escherichia coli mazg, the regulator of nutritional stress response. | mazg is a nucleoside triphosphate pyrophosphohydrolase that hydrolyzes all canonical nucleoside triphosphates. the mazg gene located downstream from the chromosomal mazef "addiction module," that mediated programmed cell death in escherichia coli. mazg activity is inhibited by the mazef complex both in vivo and in vitro. enzymatic activity of mazg in vivo affects the cellular level of guanosine 3',5'-bispyrophosphate (ppgpp), synthesized by rela under amino acid starvation. the reduction of ppgp ... | 2008 | 18353782 |
| cdna-aflp analysis of plant and pathogen genes expressed in grapevine infected with plasmopara viticola. | the oomycete plasmopara viticola (berk. and curt.) berl. and de toni causes downy mildew in grapevine (vitis vinifera l.). this pathogen is strictly biotrophic, thus completely dependent on living host cells for its survival. the molecular basis of compatibility and disease development in this system is poorly understood. we have carried out a large-scale cdna-aflp analysis to identify grapevine and p. viticola genes associated with the infection process. | 2008 | 18366764 |
| bacteria flora and heavy metals in cultivated oysters crassostrea iredalei of setiu wetland, east coast peninsular malaysia. | slipper oyster crassostrea iredalei is a species of good demand for its sweet flavor and white coloured flesh. the filter feeding nature predisposes oysters to accumulation of pathogenic and heavy metals in waters impacted by sewage pollutions and may thus render the oysters unfit for human consumption. a study was undertaken to investigate the presence of bacteria flora and heavy metal concentrations in cultivated oysters crassostrea iredalei at setiu wetland, terengganu, the only source of cul ... | 2008 | 18369732 |
| evolution of bacterial trp operons and their regulation. | survival and replication of most bacteria require the ability to synthesize the amino acid l-tryptophan whenever it is not available from the environment. in this article we describe the genes, operons, proteins, and reactions involved in tryptophan biosynthesis in bacteria, and the mechanisms they use in regulating tryptophan formation. we show that although the reactions of tryptophan biosynthesis are essentially identical, gene organization varies among species--from whole-pathway operons to ... | 2008 | 18374625 |
| genome sequence of the streptomycin-producing microorganism streptomyces griseus ifo 13350. | we determined the complete genome sequence of streptomyces griseus ifo 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. the linear chromosome consists of 8,545,929 base pairs (bp), with an average g+c content of 72.2%, predicting 7,138 open reading frames, six rrna operons (16s-23s-5s), and 66 trna genes. it contains extremely long terminal inverted repeats (tirs) of 132,910 bp each. the te ... | 2008 | 18375553 |
| gene expression of the arsenic resistance operon in chromobacterium violaceum atcc 12472. | chromobacterium violaceum atcc 12472 presents an arsrcb-type operon, which is involved in arsenic resistance. the regulating protein of this resistance system (arsr) does not have the small conserved site (elcvdcl) to link to the metalloid, as observed in escherichia coli, and is thus considered to be an atypical arsr protein, like that observed in acidithiobacillus ferrooxidans. in the present study, the gene expression profile of the ars operon under induction at different concentrations of ar ... | 2008 | 18388983 |
| rethinking proteasome evolution: two novel bacterial proteasomes. | the proteasome is a multisubunit structure that degrades proteins. protein degradation is an essential component of regulation because proteins can become misfolded, damaged, or unnecessary. proteasomes and their homologues vary greatly in complexity: from hslv (heat shock locus v), which is encoded by 1 gene in bacteria, to the eukaryotic 20s proteasome, which is encoded by more than 14 genes. despite this variation in complexity, all the proteasomes are composed of homologous subunits. we sear ... | 2008 | 18389302 |
| chromobacterium aquaticum sp. nov., isolated from spring water samples. | strain cc-seya-1t, a motile, gram-negative, non-violet-pigmented bacterium, was isolated on nutrient agar from spring-water samples collected from yang-ming mountain, taipei county, taiwan. 16s rrna gene sequence studies showed that the strain clustered with chromobacterium violaceum (96.8 % similarity) and chromobacterium subtsugae (96.5 % similarity), followed by aquitalea magnusonii (95.8 % similarity). the fatty acid profile was slightly different from those reported for c. violaceum, c. sub ... | 2008 | 18398186 |
| inactivation of [fe-s] metalloproteins mediates nitric oxide-dependent killing of burkholderia mallei. | much remains to be known about the mechanisms by which o(2)-dependent host defenses mediate broad antimicrobial activity. | 2008 | 18398486 |
| degradation of n-acyl homoserine lactone quorum sensing signal molecules by forest root-associated fungi. | a collection of mycorrhizal and nonmycorrhizal root-associated fungi coming from forest environments was screened for their ability to degrade n-acyl homoserine lactones (ahl) or to prevent ahl recognition by producing quorum sensing inhibitors (qsi). no production of qs-inhibitors or -activators was detected using the two biosensors chromobacterium violaceum cv026 and agrobacterium tumefaciens in the culture supernatant of these fungi. however, the ability to degrade c6- and 3o,c6-hsl was detec ... | 2008 | 18400006 |
| ppsy: a vector for the stable cloning and expression of streptomycete single gene phenotypes in escherichia coli. | ppsy is a 12kb cloning vector derived from the incw plasmid r388, which provides a rapid and easy way to stably clone phenotypes encoded in dna segments <10kb. in the present study three different genes were amplified by pcr, cloned into pgem-t easy and sub-cloned into the ecori site of ppsy. the first gene, vioa, is a fad-dependent l-tryptophan amino acid oxygenase from the high g+c gram-negative bacterium chromobacterium violaceum. vioa is involved in the synthesis of the indolocarbazole antit ... | 2008 | 18406459 |
| negative regulation of quorum-sensing systems in pseudomonas aeruginosa by atp-dependent lon protease. | lon protease, a member of the atp-dependent protease family, regulates numerous cellular systems by degrading specific substrates. here, we demonstrate that lon is involved in the regulation of quorum-sensing (qs) signaling systems in pseudomonas aeruginosa, an opportunistic human pathogen. the organism has two acyl-homoserine lactone (hsl)-mediated qs systems, lasr/lasi and rhlr/rhli. many reports have demonstrated that these two systems are regulated and interconnected by global regulators. we ... | 2008 | 18408026 |
| clinical immunology review series: an approach to the patient with recurrent superficial abscesses. | patients may be referred to the immunology clinic for investigation of recurrent superficial abscess formation. in the majority of adult patients this clinical presentation does not equate with an underlying primary immune deficiency. nevertheless, recurrent mucocutaneous abscesses can be associated with significant morbidity and long-term complications, including scarring and fistula formation, and may be associated with underlying immune-mediated disease. this review sets out an approach to th ... | 2008 | 18422735 |
| kinetics of thermal unfolding of phenylalanine hydroxylase variants containing different metal cofactors (feii, coii, and znii) and their isokinetic relationship. | the kinetics of thermal unfolding of apo- and holo-chromobacterium violaceum phenylalanine hydroxylase (cpah) was investigated using circular dichroism (cd) over the temperature range 44-76 degrees c. in addition to the native cofactor (feii), the unfolding kinetics of holo-cpah was characterized using znii and coii as cofactors. kinetic profiles for apo- and holo-cpah showed a single-phase exponential rise in the cd signal at lambda=222 nm and a first-order dependence on protein concentration. ... | 2008 | 18433092 |
| chromobacterium violaceum cellulitis and sepsis following cutaneous marine trauma. | chromobacterium violaceum is a gram-negative bacillary organism that characteristically produces the purple pigment violacein. documented as the cause of clinically relevant human infections in only 35 cases in the united states, c violaceum is particularly seen in patients with a history of cutaneous injury or trauma. we report the case of an 18-year-old woman who was struck by a propeller in a boating accident and sustained multiple deep lacerations of her right lower extremity. shortly after ... | 2008 | 18441852 |
| cyanogenic pseudomonads influence multitrophic interactions in the rhizosphere. | in the rhizosphere, plant roots cope with both pathogenic and beneficial bacterial interactions. the exometabolite production in certain bacterial species may regulate root growth and other root-microbe interactions in the rhizosphere. here, we elucidated the role of cyanide production in pseudomonad virulence affecting plant root growth and other rhizospheric processes. exposure of arabidopsis thaliana col-0 seedlings to both direct (with kcn) and indirect forms of cyanide from different pseudo ... | 2008 | 18446201 |
| neonatal septicaemia in calabar, nigeria. | a three-year survey of neonatal septicaemia (363 bacteriologically proven cases) in the university of calabar teaching hospital, calabar, has demonstrated that the dominant blood isolate was staphylococcus aureus (53%) followed by unclassified coliforms (20%), an unexpected chromobacterium violaceum (5%). the incidence of neonatal septicaemia was 54.9 per 1000 live births for inborn infants. the predominant organisms were largely susceptible to gentamicin, ceftriazone and cefuroxime with a morta ... | 2008 | 18453516 |
| de-orphaning the structural proteome through reciprocal comparison of evolutionarily important structural features. | function prediction frequently relies on comparing genes or gene products to search for relevant similarities. because the number of protein structures with unknown function is mushrooming, however, we asked here whether such comparisons could be improved by focusing narrowly on the key functional features of protein structures, as defined by the evolutionary trace (et). therefore a series of algorithms was built to (a) extract local motifs (3d templates) from protein structures based on et rank ... | 2008 | 18461181 |
| unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context. | the phosphoenolpyruvate phosphotransferase system (pts) plays a major role in sugar transport and in the regulation of essential physiological processes in many bacteria. the pts couples solute transport to its phosphorylation at the expense of phosphoenolpyruvate (pep) and it consists of general cytoplasmic phosphoryl transfer proteins and specific enzyme ii complexes which catalyze the uptake and phosphorylation of solutes. previous studies have suggested that the evolution of the constituents ... | 2008 | 18485189 |
| direct comparison of the bd phoenix system with the microscan walkaway system for identification and antimicrobial susceptibility testing of enterobacteriaceae and nonfermentative gram-negative organisms. | the phoenix automated microbiology system (bd diagnostics, sparks, md) is designed for the rapid identification (id) and antimicrobial susceptibility testing (ast) of clinically significant human bacterial pathogens. we evaluated the performance of the phoenix instrument in comparison with that of the microscan walkaway system (dade behring, west sacramento, ca) in the id and ast of gram-negative clinical strains and challenge isolates of enterobacteriaceae (n = 150) and nonfermentative gram-neg ... | 2008 | 18495856 |
| hydrogen peroxide linked to lysine oxidase activity facilitates biofilm differentiation and dispersal in several gram-negative bacteria. | the marine bacterium pseudoalteromonas tunicata produces an antibacterial and autolytic protein, alpp, which causes death of a subpopulation of cells during biofilm formation and mediates differentiation, dispersal, and phenotypic variation among dispersal cells. the alpp homologue (loda) in the marine bacterium marinomonas mediterranea was recently identified as a lysine oxidase which mediates cell death through the production of hydrogen peroxide. here we show that alpp in p. tunicata also act ... | 2008 | 18502869 |
| chromobacterium haemolyticum sp. nov., a strongly haemolytic species. | a gram-negative bacterium, strain mda0585(t), isolated from a sputum culture, was characterized by a polyphasic approach. the 16s rrna gene and a conserved portion of the dna gyrase a gene were sequenced and analysed phylogenetically. strain mda0585(t) showed the closest relationships with chromobacterium violaceum atcc 12472(t) and chromobacterium subtsugae praa4-1(t) (96.1 % and 96.3 % 16s rrna gene sequence similarity, respectively). the cellular fatty acids of strain mda0585(t) consisted mai ... | 2008 | 18523185 |
| epigenetic treatment of myelodysplastic syndromes and acute myeloid leukemias. | epigenetic mechanisms affecting chromatin structure contribute to regulate gene expression and assure the inheritance of information, which are essential for the proper expression of key regulatory genes in healthy cells, tissues and organs. in the medical field, an increasing body of evidence indicates that altered gene expression or de-regulated gene function lead to disease. cancer cells also suffer a profound change in the genomic methylation patterns and chromatin status. aberrant dna methy ... | 2008 | 18537607 |
| analysis of the genome sequence of lactobacillus gasseri atcc 33323 reveals the molecular basis of an autochthonous intestinal organism. | this study presents the complete genome sequence of lactobacillus gasseri atcc 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. the plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. the gc content was 35.3%, similar to the gc content of its closest relatives, l. johnsonii ncc 533 (34%) and l. acidophilus ncfm (34%). two identical copies of the prophage lgai (40,086 bp), of the sfi11- ... | 2008 | 18539810 |
| human chromobacterium violaceum infection in southeast asia: case reports and literature review. | chromobacterium violaceum infection in humans is a rare tropical and subtropical disease. the awareness of this organism is limited in spite its ubiquitous distribution. several cases have been reported from southeast asia. a localized infection followed by an overwhelming septicemia and metastatic lesions is the usual pattern of this illness. optimal antimicrobial treatment and duration are unknown. consequently, the outcome is usually fatal. the study reported two patients who suffered from fu ... | 2008 | 18564684 |
| expanding dialogues: from natural autoinducers to non-natural analogues that modulate quorum sensing in gram-negative bacteria. | bacteria are capable of "communicating" their local population densities via a process termed quorum sensing (qs). gram-negative bacteria use n-acylated l-homoserine lactones (ahls), in conjunction with their cognate luxr-type receptors, as their primary signalling circuit for qs. in this critical review, we examine ahl signalling in gram-negative bacteria with a primary focus on the design of non-natural ahls, their structure-activity relationships, and their application in chemical biological ... | 2008 | 18568169 |
| polymyxins revisited. | the global emergence of multidrug-resistant gram-negative bacilli has spurred a renewed interest in polymyxins. once discarded due to concerns regarding nephrotoxicity and neurotoxicity, polymyxins now hold an important role in the antibiotic armamentarium. however, more reliable information is needed to determine the optimal dosing of these agents. also, unanswered questions regarding in vitro testing remain, including questions regarding the reliability of automated systems and the establishme ... | 2008 | 18625681 |
| rapid hydrolysis of quorum-sensing molecules in the gut of lepidopteran larvae. | microorganisms compete for nutrients and living space in the gut of plant-feeding insect larvae, such as spodoptera spp. their physiological activities and their organization are generally controlled or synchronised by "autoinducers", such as n-acylhomoserinelactones (ahls). due to the strongly alkaline milieu in the insect gut, the lactone ring of ahls is rapidly and spontaneously opened. further degradation to the inactive components homoserine and the acyl moiety is then achieved by a microbi ... | 2008 | 18642255 |
| marine biofilm bacteria evade eukaryotic predation by targeted chemical defense. | many plants and animals are defended from predation or herbivory by inhibitory secondary metabolites, which in the marine environment are very common among sessile organisms. among bacteria, where there is the greatest metabolic potential, little is known about chemical defenses against bacterivorous consumers. an emerging hypothesis is that sessile bacterial communities organized as biofilms serve as bacterial refuge from predation. by testing growth and survival of two common bacterivorous nan ... | 2008 | 18648491 |
| quorum-sensing signals in the microbial community of the cabbage white butterfly larval midgut. | the overall goal of this study was to examine the role of quorum-sensing (qs) signals in a multispecies microbial community. toward this aim, we studied qs signals produced by an indigenous member and an invading pathogen of the microbial community of the cabbage white butterfly (cwb) larval midgut (pieris rapae). as an initial step, we characterized the qs system in pantoea cwb304, which was isolated from the larval midgut. a luxi homolog, designated pani, is necessary for the production of n-a ... | 2008 | 18650927 |
| complete genome sequence of the n2-fixing broad host range endophyte klebsiella pneumoniae 342 and virulence predictions verified in mice. | we report here the sequencing and analysis of the genome of the nitrogen-fixing endophyte, klebsiella pneumoniae 342. although k. pneumoniae 342 is a member of the enteric bacteria, it serves as a model for studies of endophytic, plant-bacterial associations due to its efficient colonization of plant tissues (including maize and wheat, two of the most important crops in the world), while maintaining a mutualistic relationship that encompasses supplying organic nitrogen to the host plant. genomic ... | 2008 | 18654632 |
| inhibition of bacterial quorum sensing-regulated behaviors by tremella fuciformis extract. | quorum sensing (qs), or the control of gene expression in response to cell density, is used by both gram-negative and gram-positive bacteria to regulate a variety of physiological functions. increasing evidence implies that certain eukaryotes produce qs-inhibitory compounds. in this work, we tested tremella fuciformis for their ability to inhibit qs-regulated behaviors. t. fuciformis fruiting bodies were dried and extracted using 75% (v/v) aqueous methanol. the crude extract was redissolved in a ... | 2008 | 18661179 |
| detection of quorum-sensing-related molecules in vibrio scophthalmi. | cell-to-cell communication (also referred to as quorum sensing) based on n-acyl-homoserine lactones (ahls) is a widespread response to environmental change in gram-negative bacteria. ahls seem to be highly variable, both in terms of the acyl chain length and in the chemical structure of the radicals. another quorum sensing pathway, the autoinducer-2-based system, is present both in gram-positive and gram-negative bacteria. in this study the presence of signal molecules belonging to both quorum s ... | 2008 | 18700048 |
| polyhydroxyalkanoate (pha) production using waste vegetable oil by pseudomonas sp. strain dr2. | to produce polyhydroxyalkanoate (pha) from inexpensive substrates by bacteria, vegetable-oil-degrading bacteria were isolated from a rice field using enrichment cultivation. the isolated pseudomonas sp. strain dr2 showed clear orange or red spots of accumulated pha granules when grown on phosphate and nitrogen limited medium containing vegetable oil as the sole carbon source and stained with nile blue a. up to 37.34% (w/w) of intracellular pha was produced from corn oil, which consisted of three ... | 2008 | 18756101 |
| brominated furanones inhibit biofilm formation by salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium is a main cause of bacterial food-borne diseases. as salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ways to inhibit biofilm formation. brominated furanones, originally extracted from the marine alga delisea pulchra, are known to interfere with biofilm formation in several pathogens. in this study, we have synthesized a small focused library of brominate ... | 2008 | 18791004 |
| fatal wound infection caused by chromobacterium violaceum in ho chi minh city, vietnam. | chromobacterium violaceum is a proteobacterium found in soil and water in tropical regions which rarely causes infection in humans. here, we report a fatal bacteremia caused by chromobacterium violaceum in vietnam. we describe a number of clinical, microbiological, and molecular aspects associated with this bacterial infection. | 2008 | 18799697 |
| successful treatment of chromobacterium violaceum sepsis in south africa. | chromobacterium violaceum sepsis is extremely rare and usually fatal. a very few cases of c. violaceum infection have been reported from africa, but never from south africa. as far as could be ascertained, this infection has never been reported in a patient with leukaemia. we describe what we believe to be the first such case of c. violaceum sepsis, in a 16-year-old female patient with acute biphenotypic leukaemia, which developed during the neutropenic phase after intensive chemotherapy. the in ... | 2008 | 18809561 |
| analysis of the pseudoalteromonas tunicata genome reveals properties of a surface-associated life style in the marine environment. | colonisation of sessile eukaryotic host surfaces (e.g. invertebrates and seaweeds) by bacteria is common in the marine environment and is expected to create significant inter-species competition and other interactions. the bacterium pseudoalteromonas tunicata is a successful competitor on marine surfaces owing primarily to its ability to produce a number of inhibitory molecules. as such p. tunicata has become a model organism for the studies into processes of surface colonisation and eukaryotic ... | 2008 | 18813346 |
| kinetic isotope effects on aromatic and benzylic hydroxylation by chromobacterium violaceum phenylalanine hydroxylase as probes of chemical mechanism and reactivity. | phenylalanine hydroxylase from chromobacterium violaceum (cvpheh) is a non-heme iron monooxygenase that catalyzes the hydroxylation of phenylalanine to tyrosine. in this study, we used deuterium kinetic isotope effects to probe the chemical mechanisms of aromatic and benzylic hydroxylation to compare the reactivities of bacterial and eukaryotic aromatic amino acid hydroxylases. the (d) k cat value for the reaction of cvpheh with [(2)h 5]phenylalanine is 1.2 with 6-methyltetrahydropterin and 1.4 ... | 2008 | 18817418 |
| comparative genomics of regulation of fatty acid and branched-chain amino acid utilization in proteobacteria. | bacteria can use branched-chain amino acids (ilv, i.e., isoleucine, leucine, valine) and fatty acids (fas) as sole carbon and energy sources converting ilv into acetyl-coenzyme a (coa), propanoyl-coa, and propionyl-coa, respectively. in this work, we used the comparative genomic approach to identify candidate transcriptional factors and dna motifs that control ilv and fa utilization pathways in proteobacteria. the metabolic regulons were characterized based on the identification and comparison o ... | 2009 | 18820024 |
| comparative genomics of regulation of fatty acid and branched-chain amino acid utilization in proteobacteria. | bacteria can use branched-chain amino acids (ilv, i.e., isoleucine, leucine, valine) and fatty acids (fas) as sole carbon and energy sources converting ilv into acetyl-coenzyme a (coa), propanoyl-coa, and propionyl-coa, respectively. in this work, we used the comparative genomic approach to identify candidate transcriptional factors and dna motifs that control ilv and fa utilization pathways in proteobacteria. the metabolic regulons were characterized based on the identification and comparison o ... | 2009 | 18820024 |
| cellulose biosynthesis by the beta-proteobacterium, chromobacterium violaceum. | the chromobacterium violaceum atcc 12472 genome was sequenced by the brazilian national genome project consortium. previous annotation reported the presence of cellulose biosynthesis genes in that genome. analysis of these genes showed that, as observed in other bacteria, they are organized in two operons. in the present work, experimental evidences of the presence of cellulose in the extracellular matrix of the biofilm produced by c. violaceum in static cultures are shown. biofilm samples were ... | 2008 | 18820969 |
| the hrp genes of pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce. | pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. through transposon insertion into p. cichorii strain spc9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. analyses showed that a transposon was inserted into the hrpg gene in 4-57 and the hrct gene in 2-99. nucleotide sequences of the hrp genes of spc9018 are homologous to those of pseudomonas viridiflava bs group strains. the pathogenicity of 4-57 on eggplant was restored by ... | 2008 | 18832299 |
| epigenetic changes in gliomas. | epigenetics are defined, in broad-terms, as alterations in gene expression without changes in dna sequence. while histone modifications and dna methylation are two classical means to regulate gene expression, mirna has also recently been documented to govern gene expression in normal as well as cancer cells. in this review, we will first describe briefly histone modifications, dna methylation and mirnas and the functions of these epigenetic marks during different cellular processes involving dna ... | 2008 | 18836290 |
| pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold spring. | a yellow-coloured bacterial strain, designated bp-5(t), was isolated from a water sample of a cold spring located in the hsinchu area of northern taiwan. cells of the strain were gram-negative, non-spore-forming rods with a polar flagellum. the isolate was able to grow at 15-42 degrees c, 0-1 % nacl and ph 6-8. the predominant fatty acids were summed feature 3 (c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh), c(16 : 0) and c(18 : 1)omega7c. the major respiratory quinone was ubiquinone 8. the dna g+c ... | 2008 | 18842860 |
| development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux. | human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. we describe the construction and development of an active surveillance of pathogens (asp) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes. | 2008 | 18844996 |
| acanthamoeba castellanii promotes the survival of vibrio parahaemolyticus. | vibrio parahaemolyticus is a food-borne pathogen that naturally inhabits both marine and estuarine environments. free-living protozoa exist in similar aquatic environments and function to control bacterial numbers by grazing on free-living bacteria. protozoa also play an important role in the survival and spread of some pathogenic species of bacteria. we investigated the interaction between the protozoan acanthamoeba castellanii and the bacterium vibrio parahaemolyticus. we found that acanthamoe ... | 2008 | 18849458 |
| ion channels in microbes. | studies of ion channels have for long been dominated by the animalcentric, if not anthropocentric, view of physiology. the structures and activities of ion channels had, however, evolved long before the appearance of complex multicellular organisms on earth. the diversity of ion channels existing in cellular membranes of prokaryotes is a good example. although at first it may appear as a paradox that most of what we know about the structure of eukaryotic ion channels is based on the structure of ... | 2008 | 18923187 |
| the pmra/pmrb two-component system of legionella pneumophila is a global regulator required for intracellular replication within macrophages and protozoa. | to examine the role of the pmra/pmrb two-component system (tcs) of legionella pneumophila in global gene regulation and in intracellular infection, we constructed pmra and pmrb isogenic mutants by allelic exchange. genome-wide microarray gene expression analyses of the pmra and pmrb mutants at both the exponential and the postexponential phases have shown that the pmra/pmrb tcs has a global effect on the expression of 279 genes classified into nine groups of genes encoding eukaryotic-like protei ... | 2009 | 18936184 |
| the pmra/pmrb two-component system of legionella pneumophila is a global regulator required for intracellular replication within macrophages and protozoa. | to examine the role of the pmra/pmrb two-component system (tcs) of legionella pneumophila in global gene regulation and in intracellular infection, we constructed pmra and pmrb isogenic mutants by allelic exchange. genome-wide microarray gene expression analyses of the pmra and pmrb mutants at both the exponential and the postexponential phases have shown that the pmra/pmrb tcs has a global effect on the expression of 279 genes classified into nine groups of genes encoding eukaryotic-like protei ... | 2009 | 18936184 |
| phase ii trial of romidepsin (nsc-630176) in previously treated colorectal cancer patients with advanced disease: a southwest oncology group study (s0336). | patients with metastatic colorectal cancer who progress on standard chemotherapy have limited treatment options. new and effective drugs are needed for these patients. romidepsin is a histone deacetylase inhibitor that can alter chromatin structure and gene transcription leading to multiple changes in cellular protein production. this may result in cell cycle arrest and tumor growth inhibition. romidepsin has shown anti-proliferative activity in vitro against multiple mouse and human tumor cell ... | 2009 | 18941712 |
| phase ii trial of romidepsin (nsc-630176) in previously treated colorectal cancer patients with advanced disease: a southwest oncology group study (s0336). | patients with metastatic colorectal cancer who progress on standard chemotherapy have limited treatment options. new and effective drugs are needed for these patients. romidepsin is a histone deacetylase inhibitor that can alter chromatin structure and gene transcription leading to multiple changes in cellular protein production. this may result in cell cycle arrest and tumor growth inhibition. romidepsin has shown anti-proliferative activity in vitro against multiple mouse and human tumor cell ... | 2009 | 18941712 |
| rrndb: documenting the number of rrna and trna genes in bacteria and archaea. | a dramatic exception to the general pattern of single-copy genes in bacterial and archaeal genomes is the presence of 1-15 copies of each ribosomal rna encoding gene. the original version of the ribosomal rna database (rrndb) cataloged estimates of the number of 16s rrna-encoding genes; the database now includes the number of genes encoding each of the rrnas (5s, 16s and 23s), an internally transcribed spacer region, and the number of trna genes. the rrndb has been used largely by microbiologist ... | 2008 | 18948294 |
| rrndb: documenting the number of rrna and trna genes in bacteria and archaea. | a dramatic exception to the general pattern of single-copy genes in bacterial and archaeal genomes is the presence of 1-15 copies of each ribosomal rna encoding gene. the original version of the ribosomal rna database (rrndb) cataloged estimates of the number of 16s rrna-encoding genes; the database now includes the number of genes encoding each of the rrnas (5s, 16s and 23s), an internally transcribed spacer region, and the number of trna genes. the rrndb has been used largely by microbiologist ... | 2008 | 18948294 |
| anthranilate synthase subunit organization in chromobacterium violaceum. | tryptophan is an aromatic amino acid used for protein synthesis and cellular growth. chromobacterium violaceum atcc 12472 uses two tryptophan molecules to synthesize violacein, a secondary metabolite of pharmacological interest. the genome analysis of this bacterium revealed that the genes trpa-f and paba-b encode the enzymes of the tryptophan pathway in which the first reaction is the conversion of chorismate to anthranilate by anthranilate synthase (as), an enzyme complex. in the present study ... | 2008 | 18949702 |
| using mahalanobis distance to compare genomic signatures between bacterial plasmids and chromosomes. | plasmids are ubiquitous mobile elements that serve as a pool of many host beneficial traits such as antibiotic resistance in bacterial communities. to understand the importance of plasmids in horizontal gene transfer, we need to gain insight into the 'evolutionary history' of these plasmids, i.e. the range of hosts in which they have evolved. since extensive data support the proposal that foreign dna acquires the host's nucleotide composition during long-term residence, comparison of nucleotide ... | 2008 | 18953039 |
| quorum sensing primes the oxidative stress response in the insect endosymbiont, sodalis glossinidius. | sodalis glossinidius, a maternally transmitted bacterial endosymbiont of tsetse flies (glossina spp.), uses an acylated homoserine lactone (ahl)-based quorum sensing system to modulate gene expression in accordance with bacterial cell density. the s. glossinidius quorum sensing system relies on the function of two regulatory proteins; sogi (a luxi homolog) synthesizes a signaling molecule, characterized as n-(3-oxohexanoyl) homoserine lactone (ohhl), and sogr1 (a luxr homolog) interacts with ohh ... | 2008 | 18958153 |
| microbial iron management mechanisms in extremely acidic environments: comparative genomics evidence for diversity and versatility. | iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake and homeostasis. information on iron management mechanisms is available for organisms living at circumneutral ph. however, very little is known about how acidophilic bacteria, especially those used for industrial copper bioleaching, cope with environmental iron loads that can be 1018 times the concentration found in ph neutral environments. t ... | 2008 | 19025650 |
| the role of histone deacetylases in prostate cancer. | epigenetic modifications play a key role in the patho-physiology of prostate cancer. histone deacetylases (hdacs) play major roles in prostate cancer progression. hdacs are part of a transcriptional co-repressor complex that influences various tumor suppressor genes. because of the significant roles played by hdacs in various human cancers, hdac inhibitors are emerging as a new class of chemotherapeutic agents. hdac inhibitors have been shown to induce cell growth arrest, differentiation and/or ... | 2008 | 19029799 |
| multiple roles of pseudomonas aeruginosa tbcf10839 pily1 in motility, transport and infection. | polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with pseudomonas aeruginosa. screening of a signature tagged mutagenesis library of the non-piliated p. aeruginosa strain tbcf10839 uncovered that transposon inactivation of its pily1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. inactivation of pily1 led to the loss of twitching motility in twitchi ... | 2009 | 19054330 |
| diversity of chromobacterium violaceum isolates from aquatic environments of state of pará, brazilian amazon. | the present study intended to characterize the phenotypic and genetic diversity of brazilian isolates of chromobacterium violaceum from aquatic environments within the amazon region. nineteen isolates showed morphological properties of c. violaceum and the majority grew at 44 degrees c. low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10 degrees c and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. ... | 2008 | 19057818 |
| improved prediction of malaria degradomes by supervised learning with svm and profile kernel. | the spread of drug resistance through malaria parasite populations calls for the development of new therapeutic strategies. however, the seemingly promising genomics-driven target identification paradigm is hampered by the weak annotation coverage. to identify potentially important yet uncharacterized proteins, we apply support vector machines using profile kernels, a supervised discriminative machine learning technique for remote homology detection, as a complement to the traditional alignment ... | 2009 | 19057851 |
| improved prediction of malaria degradomes by supervised learning with svm and profile kernel. | the spread of drug resistance through malaria parasite populations calls for the development of new therapeutic strategies. however, the seemingly promising genomics-driven target identification paradigm is hampered by the weak annotation coverage. to identify potentially important yet uncharacterized proteins, we apply support vector machines using profile kernels, a supervised discriminative machine learning technique for remote homology detection, as a complement to the traditional alignment ... | 2009 | 19057851 |
| secondary metabolites produced by the marine bacterium halobacillus salinus that inhibit quorum sensing-controlled phenotypes in gram-negative bacteria. | certain bacteria use cell-to-cell chemical communication to coordinate community-wide phenotypic expression, including swarming motility, antibiotic biosynthesis, and biofilm production. here we present a marine gram-positive bacterium that secretes secondary metabolites capable of quenching quorum sensing-controlled behaviors in several gram-negative reporter strains. isolate c42, a halobacillus salinus strain obtained from a sea grass sample, inhibits bioluminescence production by vibrio harve ... | 2009 | 19060172 |
| septicemia in a neonatal calf associated with chromobacterium violaceum. | chromobacterium violaceum infections are highly fatal and rarely reported in domestic animals. this report describes a fatal case of c. violaceum septicemia in a 7-day-old female beef calf. the calf had necrosuppurative omphalophlebitis, necrotizing interstitial pneumonia, necrosuppurative hepatitis and splenitis, anterior uveitis with hypopyon, suppurative polyarthritis, and disseminated hemorrhagic meningitis with multifocal necrotizing encephalitis. histologically, clusters of gram-negative b ... | 2009 | 19112118 |
| synchronous gene expression of the yersinia enterocolitica ysa type iii secretion system and its effectors. | type iii secretion systems (t3sss) are complex units that consist of many proteins. often the proteins are encoded as a cohesive unit on virulence plasmids, but several systems have their various components dispersed around the chromosome. the yersinia enterocolitica ysa t3ss is such a system, where the apparatus genes, some regulatory genes, and four genes encoding secreted proteins (ysp genes) are contained in a single locus. the remaining ysp genes and at least one additional regulator are fo ... | 2009 | 19124573 |