Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| analysis of agrobacterium tumefaciens plasmid ptic58 replication region with a novel high-copy-number derivative. | the origin of replication, ori, of the nopaline tumor-inducing plasmid, ptic58, mapped in a region that shares sequence homology with octopine plasmids ptiach5 and ptib6. within this region, the minimum amount of dna necessary for maintaining autonomous replication was a 2.6-kilobase region, which also comprised the incompatibility function inc. ptic58 derivatives containing inc were incompatible with agrobacterium tumefaciens plasmids ptic58, ptid1439, ptiach5, pti15955, and ptia5 and were comp ... | 1985 | 3972769 |
| the enzymatic synthesis of beta 1-2 glucans. | incubation of labeled uridine diphosphate glucose with an enzyme preparation from rhizobium meliloti or agrobacterium tumefaciens leads to the formation of a glucan which appears to be identical to the beta 1-2 cyclic glucan described by several workers. this conclusion is based on the molecular size, the formation of sophorose and higher homologs by partial acid hydrolysis, the liberation of only glucose by total acid hydrolysis, and the release of only 3,4,6-tri-o-methylglucose after methylati ... | 1985 | 3994379 |
| analysis of transfer of tumor-inducing plasmids from agrobacterium tumefaciens to petunia protoplasts. | petunia protoplasts were infected with the virulent agrobacterium tumefaciens strain a348 or the avirulent strain a136 (lacking a ti plasmid). the infection process was stopped at various time intervals up to 24 h after inoculation, and the dna from the plant cells was isolated. southern blot analysis indicated that the dna isolated from infected petunia cells was not detectably contaminated by bacterial dna from lysed agrobacterium cells. analysis of the dna from the virulent infections suggest ... | 1985 | 3997773 |
| the development of plasmid-free strains of agrobacterium tumefaciens by using incompatibility with a rhizobium meliloti plasmid to eliminate patc58. | agrobacterium tumefaciens strains lba275 and lba290 were cured of their cryptic plasmid patc58 by the introduction of the rhizobium meliloti plasmid prme41a, which is incompatible with patc58. prme41a and ptic58, the resident ti plasmid of lba275, were subsequently eliminated by growth at supraoptimal temperature (40 degrees c). the resulting plasmid-free agrobacterium strains, ubapf1 and ubapf2, have proved extremely useful for the study of rhizobium plasmids. the loss of the cryptic plasmid pa ... | 1985 | 4001194 |
| limited-host-range plasmid of agrobacterium tumefaciens: molecular and genetic analyses of transferred dna. | a tumor-inducing (ti) plasmid from a strain of agrobacterium tumefaciens that induces tumors on only a limited range of plants was characterized and compared with the ti plasmids from strains that induce tumors on a wide range of plants. whereas all wide-host-range ti plasmids characterized to date contain closely linked oncogenic loci within a single transferred dna (t-dna) region, homology to these loci is divided into two widely separated t-dna regions on the limited-host-range plasmid. these ... | 1985 | 4008445 |
| light-regulated and organ-specific expression of a wheat cab gene in transgenic tobacco. | many of our most important crop plants are monocotyledons, including wheat, corn, rice and barley. no routine transformation system for monocotyledons has been reported, such as the ti-mediated gene transfer system for dicotyledons facilitated by agrobacterium tumefaciens. indirect evidence suggests that ti-plasmid dna is transferred into and expressed in a. tumefaciens-infected wound tissues of plants from liliaceae and amaryllidaceae, but these observations have not been extended to monocotyle ... | 1985 | 4033773 |
| characterization and primary structures of dna-binding hu-type proteins from rhizobiaceae. | the dna-binding hu-type proteins from several species of rhizobiaceae including rhizobium meliloti, two strains of rhizobium leguminosarum with highly different phenotypic characters and agrobacterium tumefaciens, were characterized and their amino acid sequences were determined. hu-type proteins isolated from r. leguminosarum l18 and a. tumefaciens are identical and show slight differences with the r. meliloti hu-type protein. on the other hand the r. leguminosarum l53 hu-type protein is quite ... | 1985 | 4038648 |
| role for 2-linked-beta-d-glucan in the virulence of agrobacterium tumefaciens. | phenol-water cell extracts of virulent agrobacterium tumefaciens a348 and several avirulent mutants with a reduced ability to attach to plant surfaces were examined. a low-molecular-weight 2-linked-beta-d-glucan was identified in the cell wall extracts of the virulent wild-type strain. analyses of phenol-water extracts and culture filtrates of four mutant strains showed that the mutants did not produce any 2-linked-beta-d-glucan. when these mutants were complemented, the ability to produce the g ... | 1985 | 4044517 |
| molecular and genetic analysis of the transferred dna regions of the root-inducing plasmid of agrobacterium rhizogenes. | the t-dna regions of the root-inducing (ri) plasmid pria4b of agrobacterium rhizogenes were characterized. two regions, designated tl-dna and tr-dna, were found to be integrated and stably maintained in the plant genome. the tl-dna spanned a 15- to 20-kilobase region of pria4b and was separated from the tr-dna region by at least 15 kilobases of nonintegrated plasmid dna. the tr-dna region also spanned a 15- to 20-kilobase region of pria4b and included a region of homology to the tms morphogenic ... | 1985 | 4044524 |
| regions of broad-host-range plasmid rk2 involved in replication and stable maintenance in nine species of gram-negative bacteria. | the replication and maintenance properties of the broad-host-range plasmid rk2 and its derivatives were examined in nine gram-negative bacterial species. two regions of rk2, the origin of replication (oriv) and a segment that encodes for a replication protein (trfa delta kild, designated trfa*), are sufficient for replication in all nine species tested. however, stable maintenance of this minimal replicon (less than 0.3% loss per generation under nonselection conditions) is observed only in esch ... | 1985 | 4044529 |
| new class of limited-host-range agrobacterium mega-tumor-inducing plasmids lacking homology to the transferred dna of a wide-host-range, tumor-inducing plasmid. | biotype 1 and 2 strains of agrobacterium tumefaciens were isolated from crown gall tumors of lippia canescens plants growing as ground cover in arizona. the isolates were agrocin 84 sensitive, did not catabolize octopine, nopaline, agropine, or mannopine, and were limited in their tumorigenic host range. one biotype 2 strain, ab2/73, showed the most limited host range; it incited tumors only on lippia strains, the cucurbit family of plants, and nicotiana glauca. megaplasmids were detected in the ... | 1985 | 4055698 |
| regulation of ti plasmid virulence genes by a chromosomal locus of agrobacterium tumefaciens. | we isolated a mutant strain of agrobacterium tumefaciens, designated ros, that has a pleiotropic phenotype which includes elevated levels of expression of certain genes in the virulence (vir) region of tumor-inducing plasmid ptic58. this mutant and others were isolated by fusing the promoter of the vir bak gene to a promoterless gene (cat) that encodes chloramphenicol acetyltransferase and then selecting for increased expression of cat in a. tumefaciens. the ros mutation is chromosomal in nature ... | 1985 | 4055699 |
| selection-expression plasmid vectors for use in genetic transformation of higher plants. | plasmid vectors containing both a selectable marker for plant transformation (kanamycin resistance) and a second, directly adjacent, divergent promoter for the transcription of inserted dna fragments have been constructed. these vectors make use of a small (479 bp) dual-promoter dna fragment, originally isolated from the t-dna of agrobacterium tumefaciens, fused to the neomycin phosphotransferase gene of tn5. several unique restriction enzyme cleavage sites, as well as a polyadenylation signal s ... | 1985 | 4059052 |
| identification of a rhizobium meliloti psym2011 region controlling the host specificity of root hair curling and nodulation. | in rhizobium meliloti 2011 nodulation genes (nod) required to nodulate specifically alfalfa are located on a psym megaplasmid. nod- derivatives carrying large psym deletions were isolated. by complementation of these strains with in vivo- and in vitro-constructed episomes containing psym of sequences and introduction of these episomes into agrobacterium tumefaciens, we show (i) that from a region of psym of about 360 kilobases, genes required for specific alfalfa nodulation are clustered in a dn ... | 1985 | 4066612 |
| humoral immunity against francisella tularensis after natural infection. | forty-two subjects with acute tularemia were studied for the occurrence of c-reactive protein (crp), and 73 subjects with acute tularemia or experience of the disease within the last 11 years were studied for immunoglobulin m (igm), iga, and igg class-specific antibodies, agglutinating antibodies, and complement-fixing antibodies to francisella tularensis by using an enzyme-linked immunosorbent assay (elisa), the tube agglutination test, and a complement-fixing elisa. the incubation time between ... | 1985 | 4066925 |
| septicemia caused by agrobacterium sp. | agrobacterium radiobacter biovar 2 was repeatedly grown from the blood of an elderly patient receiving artificial ventilation and broad-spectrum antibiotics. no source of the organism was found, but the septicemia ceased when cefotaxime was given. sera from the patient showed a fourfold rise in antibody against the organism and higher titers than sera from all but 1 of 50 healthy blood donors. the organism did not contain the plasmid associated with plant oncogenicity. this case may be only the ... | 1985 | 4077973 |
| heterologous hybridization of bacterial dna to the endoglucanases a and b structural genes cela and celb of clostridium thermocellum. | dna from various cellulolytic and non-cellulolytic bacteria was found to hybridize to clostridium thermocellum ncib10682 dna fragments carrying the structural genes cela and celb which code for endoglucanases a and b. homology to cela was detected in agrobacterium rhizogenes, azospirillum brasilense, bacillus subtilis, cellulomonas sp., clostridium stercorarium, erwinia chrysanthemi, pseudomonas solanacearum and streptomyces griseus. homology to celb was detected only in b. subtilis, c. stercora ... | 1985 | 4083831 |
| n2-(1-carboxyethyl)methionine. a 'pseudo-opine' in octopine-type crown-gall tumours. | a novel methionine-containing plasmid-determined compound, n2-(1-carboxyethyl)methionine (ncem) has been identified in crown-gall tumours induced by octopine-type strains of agrobacterium tumefaciens. ncem is probably synthesized by octopine synthase. cell-free preparations from octopine-type strains of a. tumefaciens can degrade ncem; however, the bacterium cannot transport the compound into the cell, although these strains can take up and degrade the octopine family of opines. | 1985 | 4091800 |
| the use of pnj5000 as an intermediate vector for the genetic manipulation of agrobacterium ti-plasmids. | the use of broad-host-range plasmids derived from rp4 as intermediate vectors for the transfer of narrow-host-range recombinant plasmids from escherichia coli to agrobacterium tumefaciens as a preliminary to marker exchange is described. recombinant plasmids having a cole1 type origin were linked to the rp4 derivative. cointegrate formation appeared to take place by reca-independent, homologous recombination within a short piece of dna derived from the beta-lactamase gene of tn1/tn3 carried by b ... | 1985 | 4093762 |
| electron microscopy of phages for agrobacterium tumefaciens. | 1970 | 4098556 | |
| morphological, biological, and physical properties of agrobacterium tumefaciens bacteriophages. | 1972 | 4110281 | |
| a soluble cytochrome c from agrobacterium tumefaciens, strain b2a. | 1973 | 4128307 | |
| "genetic' transformation of bacteria by rna and loss of oncogenic power properties of agrobacterium tumefaciens. transforming rna as template for dna synthesis. | 1973 | 4137363 | |
| 3-ketoglucose reductase of agrobacterium tumefaciens. | two kinds of 3-ketoglucose-reducing enzyme were partially purified from the sonic extract of agrobacterium tumefaciens iam 1525 grown on a sucrose-containing medium. both enzymes have a specific requirement for reduced nicotinamide adenine dinucleotide phosphate (nadph) as a hydrogen donor and catalyze the reduction of 3-ketoglucose to glucose but do not reduce 3-ketoglucosides such as 3-ketosucrose, 3-ketoglucose-1-phosphate, 3-ketotrehalose, and 3-ketocellobiose. from the requirement and subst ... | 1973 | 4144143 |
| purification & properties of a soluble reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase from agrobacterium tumefaciens. | 1971 | 4146370 | |
| [ultrastructure of some bacteriophages of agrobacterium tumefaciens]. | 1974 | 4214287 | |
| mechanism of nitrate reduction by agrobacterium tumefaciens. | 1964 | 4243407 | |
| [bacterial cell wall and tumor induction by agrobacterium tumefaciens (author's transl)]. | 1973 | 4271607 | |
| investigations implying the invalidity of octopine as a marker for transformation by agrobacterium tumefaciens. | 1973 | 4273701 | |
| [8-methoxypsoralen makes agrobacterium tumefaciens, kalanchoe daigremontiana, and the tumor induction sensitive for long wave uv (author's transl)]. | 1974 | 4277973 | |
| nadh oxidase system of agrobacterium tumefaciens. | 1966 | 4287666 | |
| cytochrome c552 in agrobacterium tumefaciens. | 1968 | 4302139 | |
| conversion of glucose-1-phosphate to 3-keto-glucose-1-phosphate by cells of agrobacterium tumefaciens. | incubation of resting cells of agrobacterium tumefaciens with glucose-1-phosphate resulted in the accumulation of a new sugar phosphate in the suspending medium. approximately 80% of the glucose-1-phosphate consumed was converted to the new compound, which was identified as alpha-d-ribo-hexopyranosyl-3-ulose-1-phosphate (3-ketoglucose-1-phosphate). both utilization of glucose-1-phosphate and accumulation of 3-ketoglucose-1-phosphate were inhibited by 2,4-dinitrophenol, polymyxin, and d-glucose, ... | 1969 | 4304223 |
| hexuronic acid dehydrogenase of agrobacterium tumefaciens. | growth of agrobacterium tumefaciens on d-glucuronic acid (glcua) or d-galacturonic acid (galua) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (nad) oxidoreductase]. the dehydrogenase, which irreversibly converts glcua or galua to the corresponding hexaric acid with the concomitant reduction of nad, but not of nicotinamide adenine dinucleotide phosphate was purified 60-fold by mncl(2) treatment, (nh(4))(2)so(4) fractionation, chromatogr ... | 1969 | 4313130 |
| d-glucaric acid and galactaric acid catabolism by agrobacterium tumefaciens. | cell-free extract (crude extract) of agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. these compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. the same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine ... | 1970 | 4314480 |
| control of pantothenate accumulation in agrobacterium tumefaciens. | two pantothenate-requiring mutants of agrobacterium tumefaciens have been isolated. one of them (strain wmp-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. extracts of cells grown on limiting aspartate were found to contain four times more (14)c-pantothenate than those grown at optimal aspartate concentrations. this difference was found in ... | 1973 | 4347924 |
| [inhibition of agrobacterium tumefaciens by d-amino acids]. | 1965 | 4380220 | |
| [3. the influence of rifampicin on tumor induction by agrobacterium tumefaciens]. | 1970 | 4394213 | |
| [iv. rifampicin and a resistant clone of agrobacterium tumefaciens in tumor induction]. | 1970 | 4395544 | |
| [rifampicin and the agrobacterium tumefaciens phage ps8]. | 1972 | 4403419 | |
| [ -amanitin inhibits tumor induction by agrobacterium tumefaciens]. | 1972 | 4405303 | |
| large plasmid in agrobacterium tumefaciens essential for crown gall-inducing ability. | 1974 | 4419109 | |
| the presence of defective prophages in agrobacterium tumefaciens, strains 806 and b6s. | 1974 | 4437681 | |
| studies on the bacteriophage ps-8 of agrobacterium tumefaciens (smith and townsend) conn: purification and properties. | 1974 | 4451429 | |
| unidirectional growth and branch formation of a morphological mutant, agrobacterium tumefaciens. | morphological characteristics of thermoconditional mutant agrobacterium tumefaciens f-502 were investigated in relation to growth, division, and synthesis of cellular components. as a result of a shift from 27 to 37 c, mutant cells altered their morphology from short rods to elongated and branched forms; in addition, division and deoxyribonucleic acid synthesis were inhibited at 37 c. at 37 c unidirectional cell growth and branch formation occurred at one end of a cell, and the elongation rate o ... | 1974 | 4455684 |
| the nucleoside triphosphate-ribonucleic acid nucleotidyltransferase (ec 2.7.7.6) of agrobacterium tumefaciens (smith and townsend) conn. purification and properties of the enzyme from the tumorigenic strain b6806. | the rna nucleotidyltransferase (rna polymerase) of the plant-tumorigenic bacterium agrobacterium tumefaciens was purified. the method involves the disruption of the bacterial cells with glass beads in a waring blendor, treatment with deae-cellulose, fractionation with (nh(4))(2)so(4), protamine sulphate precipitation, deae-cellulose column chromatography and either glycerol-gradient centrifugation or phosphocellulose chromatography. the subunit structure of the highly purified enzyme is similar ... | 1974 | 4462737 |
| [antibody formation against agrobacterium tumefaciens in patients with various cancers]. | 1974 | 4463498 | |
| transmissible resistance to penicillin g, neomycin, and chloramphenicol in rhizobium japonicum. | the genetic basis for resistance to a number of antibiotics was examined in rhizobium japonicum. resistance to penicillin g, neomycin, and chloramphenicol appears to be mediated by an extrachromosomal element similar to that found in the enterobacteriaceae. resistance to these antibiotics was eliminated from cells by treatment with acridine orange, and resistance to all three antibiotics could be transferred en bloc to agrobacterium tumefaciens under conditions excluding transformation or transd ... | 1973 | 4491197 |
| isolation of the tumor-inducing rna from oncogenic and nononcogenic agrobacterium tumefaciens. | two rna fractions have been isolated and purified from both oncogenic and nononcogenic strains of agrobacterium tumefaciens. both rnas are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of datura stramonium plants. one of these rna fractions was found to be bound to an rna-directed dna polymerase, while the other was associated with the bacterial dna. physical evidence suggests that both are single stranded and small in size; linear sucrose gra ... | 1974 | 4525450 |
| agrobacterium tumefaciens dna and ps8 bacteriophage dna not detected in crown gall tumors. | renaturation kinetics of labeled agrobacterium tumefaciens dna are not influenced by addition of 10(4)-fold excess of crown gall tumor dna. reconstruction experiments demonstrated that 0.01% added bacterial dna produces a detectable increase in rate of renaturation of labeled dna. crown gall tumor dna therefore cannot contain as much as 0.01% a. tumefaciens dna (one entire bacterial genome per three diploid tumor cells). by the same technique, ps8 bacteriophage dna is not detected in crown gall ... | 1974 | 4530328 |
| quantitative estimation of agrobacterium tumefaciens dna in crown gall tumor cells. | several reports suggest that agrobacterium tumefaciens nucleic acids can induce transformation of the cells of susceptible host plants and that bacteria-free tissue cultures of transformed cells contain a. tumefaciens dna, rna, antigens, or bacteriophages. we assayed vinca rosea tumor dna for base sequence homologies with a. tumefaciens dna by dna.dna solution enrichment and dna.dna filter saturation hybridization techniques. no homologies were found by either method. the filter saturation hybri ... | 1974 | 4530329 |
| transformation of agrobacterium tumefaciens into a non-oncogenic species by an escherichia coli rna. | transforming rna excreted by showdomycin-resistant escherichia coli induces a persistent, heritable, and spectacular change in agrobacterium tumefaciens b(6), a bacterium that carries the oncogenic principle for tumor induction in plants. transformants possessing new physiological and biochemical properties have completely or partially lost the capacity for tumor induction. they synthetize new ribosomes whose components are profoundly modified. on the basis of biological and biochemical characte ... | 1972 | 4550504 |
| isolation of morphological mutants of agrobacterium tumefaciens. | morphological mutants were isolated from a wild strain of agrobacterium tumefaciens at a high frequency by treatment with a nitrosoguanidine. seventeen of the 20 mutants isolated were temperature-sensitive. at 27 c, the mutant cells were rod-shaped and at 37 c, spherical or branched, whereas the wild-type cells were rod-shaped at both temperatures. | 1972 | 4553841 |
| characteristics of greek isolates of agrobacterium tumefaciens (e. f. smith & townsend) conn. | 1973 | 4583867 | |
| [effect of bacterial attenuation on expression of the oncogenic capacity of agrobacterium tumefaciens]. | 1972 | 4628302 | |
| [relationship between l-asparaginase activity and the oncogenic power of different agrobacterium tumefaciens mutants (smith and town.) conn]. | 1972 | 4630665 | |
| [loss of virulence associated with loss of an enzyme activity in agrobacterium tumefaciens]. | 1972 | 4631534 | |
| ultrastructure of stable l phase of agrobacterium tumefaciens causing tumor fromation in phaseolus vulgaris l. | 1972 | 4663991 | |
| [structure and chemical composition of lipopolysaccharides of l forms of agrobacterium tumefaciens]. | 1972 | 4664780 | |
| electron microscopy of lipopolysaccharides from erwinia carotovora and the bacillary and l forms of agrobacterium tumefaciens. | 1972 | 4673374 | |
| [relationship between l-asparaginase activity and the oncogenic power of different agrobacterium tumefaciens mutants (smith and town.) conn]. | 1973 | 4684029 | |
| on the question of crown-gall tumor initiation by dna of bacteriophage ps8. | dna of agrobacterium tumefaciens bacteriophage ps8 was isolated by using several procedures. whole phage and phage dna were tested for tumor-inducing ability on 10 species of plants with various additions to assist such activity. the reported tumorigenicity of phage ps8 dna could not be confirmed, and no evidence to implicate phage ps8 involvement in tumor initiation was obtained. | 1973 | 4688706 |
| glucose-1-phosphate-negative mutant of agrobacterium tumefaciens. | glucose-1-phosphate-negative mutants that are unable to grow in a synthetic medium containing glucose-1-phosphate (g-1-p) as a sole carbon source were isolated by treatment of agrobacterium tumefaciens iam 1525 with n-methyl-n'-nitro-n-nitrosoguanidine. all of the enzymes involved in g-1-p metabolism (glucoside-3-dehydrogenase, 3-ketoglucose-1-phosphate-degrading enzyme, alpha-glucosidase, and phosphatases) were detected in the sonic extract prepared from resting cells of one of the mutants, str ... | 1973 | 4690962 |
| the presence of both phage ps8 and agrobacterium tumefaciens a 6 dna base sequences in a 6 -induced sterile crown-gall tissue cultured in vitro. | 1973 | 4691351 | |
| [specific degradation of octopine and arginine by virulent agrobacterium tumefaciens b6]. | 1973 | 4725823 | |
| carbohydrate metabolism in agrobacterium tumefaciens. | the activity of pentose cycling (pc) reactions in agrobacterium tumefaciens is much greater than that normally found in bacteria, and in this regard the organism represents a unique category. equations specifically derived from radiorespirometric data for bacteria with high pc activity in the presence of an alternate pathway are presented. a. tumefaciens utilizes d-glucose by strictly aerobic mechanisms involving the entner-doudoroff (ed) and pc pathways; relative participation by the ed pathway ... | 1973 | 4745418 |
| ["agrobacterium tumefaciens" (smith and town) conn.: minimal inhibitory concentrations of various antibiotics and sulphonamide (author's transl)]. | 1973 | 4748294 | |
| induction of crown gall by nitrosoguanidine-treated agrobacterium tumefaciens. | 1973 | 4753932 | |
| purification and properties of glucose i-phosphate-binding protein in agrobacterium tumefaciens. | 1973 | 4761987 | |
| host-bacteriophage interaction in agrobacterium tumefaciens. 3. phage-coded endolysins. | endolysins were detected in a sensitive strain of agrobacterium tumefaciens (b6) after infection with phage lv-1 and in the lysogen a. tumefaciens v-1 after induction with mitomycin c. a similar endolysin was found in mitomycin c-induced a. tumefaciens c-58, which apparently harbors a defective prophage. | 1974 | 4821492 |
| host-phage interaction in agrobacterium tumefaciens. ii. host strain response to mitomycin c induction. | 1974 | 4822054 | |
| attempts to detect deoxyribonucleic acid from agrobacterium tumefaciens and bacteriophage ps8 in crown gall tumors by complementary ribonucleic acid-deoxyribonucleic acid-filter hybridization. | labeled ribonucleic acid (rna) complementary to agrobacterium tumefaciens dna and ps8 bacteriophage dna (crna) were used in a systematic study of the sensitivity of crna/deoxyribonucleic acid (dna)-filter hybridization for detection of small amounts of phage or bacterial dna immobilized on filters. a. tumefaciens crna of specific activity 10(6) to 2 x 10(6) counts per min per mug reacted to a significant extent when the dna-filter contained 1% a. tumefaciens dna in a salmon dna background, but 0 ... | 1974 | 4850689 |
| [ribonucleic acids in crown-gall agrobacterium tumefaciens (smith and town) conn. bacteria exposed to sodium borate]. | 1968 | 4878338 | |
| the nature, intergeneric distribution and biosynthesis of isoprenoid quinones and phenols in gram-negative bacteria. | 1. twenty-two aerobically grown gram-negative bacteria were analysed for demethylmenaquinones, menaquinones, 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols and ubiquinones. 2. all the eight enterobacteria and both the two facultative organisms (aeromonas punctata and aeromonas hydrophila) examined contain all the compounds listed above. the principal homologues are octaprenyl; in addition lower (down to tri- or tetra-prenyl for the 2-polyprenylphenols) and sometimes higher homologues are als ... | 1969 | 4886765 |
| conditions for production of 3-ketomaltose from agrobacterium tumefaciens. | up to 39% yields of 3-ketomaltose were achieved in 18 to 22 hr when agrobacterium tumefaciens nrrl b-36 was cultured at 25 to 28 c in a simple medium containing 4.0 to 8.0% maltose, 0.09% urea, 0.5% caco(3), 0.6% kh(2)po(4), and 0.025% mgso(4).7h(2)o. for maximum production of 3-ketomaltose the culture had to be maintained approximately at ph 7.0. | 1971 | 4925530 |
| a selective medium for agrobacterium radiobacter biotype 2. | 1971 | 4935439 | |
| [on the variation of the morphology of populations of agrobacterium tumefaciens during their proliferation in different media]. | 1966 | 4955686 | |
| deoxyribonucleic acid homology and taxonomy of agrobacterium, rhizobium, and chromobacterium. | hybridization experiments were carried out between high molecular weight, denatured, agar-embedded deoxyribonucleic acid (dna) and homologous, nonembedded, sheared, denatured (14)c-labeled dna from a strain of agrobacterium tumefaciens and rhizobium leguminosarum (the reference strains) in the presence of sheared, nonembedded, nonlabeled dna (competing dna) from the same or different nomen-species of agrobacterium, rhizobium, chromobacterium, and several other organisms. percentage of dna homolo ... | 1967 | 4961629 |
| serological comparisons between strains of agrobacterium tumefaciens. | 1967 | 4962688 | |
| [on the transformation of normal tissue of viper's-grass in vitro induced by deoxyribonucleic acid of agrobacterium tumefaciens]. | 1967 | 4966529 | |
| suspected mutant of agrobacterium tumefaciens with an altered dna. | 1969 | 4979962 | |
| serological studies with agrobacterium radiobacter, a.tumefaciens, and rhizobium strains. | 1971 | 4999394 | |
| transfection in agrobacterium tumefaciens. | intact cells of agrobacterium tumefaciens were examined for ability to take up biologically active lr-4 phage deoxyribonucleic acid (dna) from the surrounding medium. dna incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts media, and was restricted to a 4-hr period in the early log phase of growth in enriched media. in the latter case, maximal transfection frequencies were obtained after a 25- to 30-min inc ... | 1972 | 5040385 |
| [various tarnished facts about agrobacterium tumefaciens]. | 1972 | 5068500 | |
| host-bacteriophage interaction in agrobacterium tumefaciens. i. characterization of bacteriophage r4 and physiological changes in the infected host cell. | infection of agrobacterium tumefaciens b6, a tumor-producing plant pathogen, by bacteriophage r4, does not immediately shut off host deoxyribonucleic acid (dna), ribonucleic acid (rna), and protein synthesis. viral dna synthesis begins soon after infection, but the host dna is not shut off until after 35 min; net rna and protein synthesis are not inhibited until 30 min after infection. the pattern of synthesis of phage particles was confirmed by electron microscopy of thin sections during the in ... | 1972 | 5084466 |
| [induction of tumors by the thermostable endotoxin of agrobacterium tumefaciens on datura stramonium l]. | 1971 | 5129961 | |
| [structure and chemical composition of the lipopolysaccharide of agrobacterium tumefaciens]. | 1971 | 5159956 | |
| tumor induction by agrobacterium tumefaciens: specific transfer of bacterial deoxyribonucleic acid to plant tissue. | when agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (dna) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. such association is not observed in identical experiments when a. tumefaciens cell ribonucleic acid or protein are labeled. the extent of the retention of thymidine-derived label from bacterial cells by t ... | 1971 | 5167810 |
| ribonuclease induction in cells transformed by agrobacterium tumefaciens. | 1966 | 5230147 | |
| agrobacterium tumefaciens rna in non-tumorous tomato cells. | 1969 | 5353894 | |
| active transport of uridine diphosphate glucose in agrobacterium tumefaciens. | 1969 | 5366997 | |
| [studies on the production of a cytokinic substance by agrobacterium tumefaciens (smith and town) conn]. | 1969 | 5373660 | |
| growth requirements and infectivity of auxotrophic adenine-dependent mutants of agrobacterium tumefaciens. | 1969 | 5374246 | |
| [interspecific transformations of agrobacterium tumefaciens and rhizobium leguminosarum]. | 1969 | 5384628 | |
| [electron microscopy of tumor tissue in phaseolus vulgaris by agrobacterium tumefaciens]. | 1969 | 5386306 | |
| anomaly in the neutralization kinetics of phages of agrobacterium radiobacter. | 1969 | 5392738 | |
| [antagonistic relationship between actinomycetes and agrobacterium tumefaciens (smith and townsend) conn. i]. | 1969 | 5398252 | |
| physicochemical properties of agrobacterium tumefaciens phage lv-1 and its dna. | 1970 | 5411193 | |
| [observations on the presence of phages in wounds infected with different strains of agrobacterium tumefaciens (smith and town) conn]. | 1970 | 5422870 | |
| active transport of glucose-1-phosphate in agrobacterium tumefaciens. | the presence of an active transport system for glucose-1-phosphate in agrobacterium tumefaciens was demonstrated from the following observations. (i) the bacterium could grow on a medium containing glucose-1-phosphate as carbon source; (ii) the entry of glucose-1-phosphate into the resting cells occurred against concentration gradient obeying michaelis-menten kinetics; and (iii) the entry reaction was energy-dependent. the transport system for glucose-1-phosphate was formed inducibly by growing ... | 1970 | 5438042 |