Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacterial chemotaxis to atrazine and related s-triazines. | pseudomonas sp. strain adp utilizes the human-made s-triazine herbicide atrazine as the sole nitrogen source. the results reported here demonstrate that atrazine and the atrazine degradation intermediates n-isopropylammelide and cyanuric acid are chemoattractants for strain adp. in addition, the nonmetabolized s-triazine ametryn was also an attractant. the chemotactic response to these s-triazines was not specifically induced during growth with atrazine, and atrazine metabolism was not required ... | 2009 | 19581468 |
| conformational dynamics in the acyl-coa synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase. | the anl superfamily of adenylating enzymes contains acyl- and aryl-coa synthetases, firefly luciferase, and the adenylation domains of the modular non-ribosomal peptide synthetases (nrpss). members of this family catalyze two partial reactions: the initial adenylation of a carboxylate to form an acyl-amp intermediate, followed by a second partial reaction, most commonly the formation of a thioester. recent biochemical and structural evidence has been presented that supports the use by this enzym ... | 2009 | 19610673 |
| evolution of efficient pathways for degradation of anthropogenic chemicals. | anthropogenic compounds used as pesticides, solvents and explosives often persist in the environment and can cause toxicity to humans and wildlife. the persistence of anthropogenic compounds is due to their recent introduction into the environment; microbes in soil and water have had relatively little time to evolve efficient mechanisms for degradation of these new compounds. some anthropogenic compounds are easily degraded, whereas others are degraded very slowly or only partially, leading to a ... | 2009 | 19620997 |
| examination of enzymatic h-tunneling through kinetics and dynamics. | in recent years, kinetic measurements of isotope effects of enzyme-catalyzed reactions and their temperature dependence led to the development of theoretical models that were used to rationalize the findings. these models suggested that motions at the femto- to picosecond (fs to ps) time scale modulate the environment of the catalyzed reaction. due to the fast nature of motions that directly affect the cleavage of a covalent bond, it is challenging to correlate the enzyme kinetics and dynamics r ... | 2009 | 19621965 |
| distribution of bacterial keratitis and emerging resistance to antibiotics in china from 2001 to 2004. | to study on the distribution of bacterial keratitis isolates and the resistance to antibiotics in china from 2001 to 2004. | 2008 | 19668756 |
| the role of the conserved phenylalanine in the sigma54-interacting gaftga motif of bacterial enhancer binding proteins. | sigma(54)-dependent transcription requires activation by bacterial enhancer binding proteins (bebps). bebps are members of the aaa+ (atpases associated with various cellular activities) protein family and typically form hexameric structures that are crucial for their atpase activity. the precise mechanism by which the energy derived from atp hydrolysis is coupled to biological output has several unknowns. here we use escherichia coli pspf, a model bebp involved in the transcription of stress res ... | 2009 | 19692583 |
| organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent pseudomonas. | phosphorus deficiency is a major constraint to crop production due to rapid binding of the applied phosphorus into fixed forms not available to the plants. microbial solubilization of inorganic phosphates has been attributed mainly to the production of organic acids. phosphate-solubilizing microorganisms enhance plant growth under conditions of poor phosphorus availability by solubilizing insoluble phosphates in the soil. this paper describes the production of organic acids during inorganic phos ... | 2009 | 19698133 |
| isolation and characterization of a novel 2-sec-butylphenol-degrading bacterium pseudomonas sp. strain ms-1. | a novel bacterium capable of utilizing 2-sec-butylphenol as the sole carbon and energy source, pseudomonas sp. strain ms-1, was isolated from freshwater sediment. within 30 h, strain ms-1 completely degraded 1.5 mm 2-sec-butylphenol in basal salt medium, with concomitant cell growth. a pathway for the metabolism of 2-sec-butylphenol by strain ms-1 was proposed on the basis of the identification of 3 internal metabolites-3-sec-butylcatechol, 2-hydroxy-6-oxo-7-methylnona-2,4-dienoic acid, and 2-me ... | 2010 | 19705287 |
| initial insight into the function of the lysosomal 66.3 kda protein from mouse by means of x-ray crystallography. | the lysosomal 66.3 kda protein from mouse is a soluble, mannose 6-phosphate containing protein of so far unknown function. it is synthesized as a glycosylated 75 kda precursor that undergoes limited proteolysis leading to a 28 kda n- and a 40 kda c-terminal fragment. | 2009 | 19706171 |
| temperature-dependent expression of phzm and its regulatory genes lasi and ptsp in rhizosphere isolate pseudomonas sp. strain m18. | pseudomonas sp. strain m18, an effective biological control agent isolated from the melon rhizosphere, has a genetic background similar to that of the opportunistic human pathogen pseudomonas aeruginosa pao1. however, the predominant phenazine produced by strain m18 is phenazine-1-carboxylic acid (pca) rather than pyocyanin (pyo); the quantitative ratio of pca to pyo is 105 to 1 at 28 degrees c in strain m18, while the ratio is 1 to 2 at 37 degrees c in strain pao1. we first provided evidence th ... | 2009 | 19717631 |
| oral microbial and respiratory status of persons with mental retardation/intellectual and developmental disability: an observational cohort study. | the objective of this study was to determine the prevalence of select microorganisms in oral biofilms and to investigate relationships between oral and respiratory status in persons with mental retardation/intellectual and developmental disabilities (idd). | 2009 | 19748295 |
| the effects of maturation on the colonic microflora in infancy and childhood. | the composition of colonic mircoflora and its changes with maturation have rarely been investigated in large samples. methods. we used conventional microbiological testing to analyse the colonic flora (kyberstatus, institut formicroecology, herborn, germany) of stool samples from 12 484 children with different intestinal and nonintestinal diagnoses. stool samples were analysed for total colony forming units (cfu) (per g stool) and the abundance of bifidobacteria, bacteroides sp., escherichia col ... | 2009 | 19763278 |
| incidence and severity of respiratory syncytial virus pneumonia in rural kenyan children identified through hospital surveillance. | although necessary for developing a rationale for vaccination, the burden of severe respiratory syncytial virus (rsv) disease in children in resource-poor settings remains poorly defined. | 2009 | 19788358 |
| health-care associated infections rates, length of stay, and bacterial resistance in an intensive care unit of morocco: findings of the international nosocomial infection control consortium (inicc). | most studies related to healthcare-associated infection (hai) were conducted in the developed countries. we sought to determine healthcare-associated infection rates, microbiological profile, bacterial resistance, length of stay (los), and extra mortality in one icu of a hospital member of the international infection control consortium (inicc) in morocco. | 2009 | 19811636 |
| prevalence of renal anomalies after urinary tract infections in hospitalized infants less than 2 months of age. | our aim was to determine the incidence of anatomical abnormalities after a urinary tract infection (uti) in infants <2 months of age hospitalized in the neonatal intensive care unit (nicu). | 2010 | 19812586 |
| prevalence of renal anomalies after urinary tract infections in hospitalized infants less than 2 months of age. | our aim was to determine the incidence of anatomical abnormalities after a urinary tract infection (uti) in infants <2 months of age hospitalized in the neonatal intensive care unit (nicu). | 2010 | 19812586 |
| tonb-dependent transporters and their occurrence in cyanobacteria. | different iron transport systems evolved in gram-negative bacteria during evolution. most of the transport systems depend on outer membrane localized tonb-dependent transporters (tbdts), a periplasma-facing tonb protein and a plasma membrane localized machinery (exbbd). so far, iron chelators (siderophores), oligosaccharides and polypeptides have been identified as substrates of tbdts. for iron transport, three uptake systems are defined: the lactoferrin/transferrin binding proteins, the porphyr ... | 2009 | 19821963 |
| crystal structure and functional analysis of the extradiol dioxygenase lapb from a long-chain alkylphenol degradation pathway in pseudomonas. | lapb is a non-heme fe(ii)-dependent 2,3-dioxygenase that catalyzes the second step of a long-chain alkylphenol (lap) degradation pathway in pseudomonas sp. kl28 and belongs to the superfamily of type i extradiol dioxygenases. in this study, the crystal structures of substrate-free lapb and its complexes with a substrate or product were determined, along with a functional analysis of the active site residues. structural features of the homotetramer are similar to those of other type i extradiol d ... | 2009 | 19828456 |
| fabrication and modeling of dynamic multipolymer nanofibrous scaffolds. | aligned nanofibrous scaffolds hold tremendous potential for the engineering of dense connective tissues. these biomimetic micropatterns direct organized cell-mediated matrix deposition and can be tuned to possess nonlinear and anisotropic mechanical properties. for these scaffolds to function in vivo, however, they must either recapitulate the full dynamic mechanical range of the native tissue upon implantation or must foster cell infiltration and matrix deposition so as to enable construct matu ... | 2009 | 19831482 |
| accounting for mating pair formation in plasmid population dynamics. | plasmids are important vehicles for horizontal gene transfer and rapid adaptation in bacteria, including the spread of antibiotic resistance genes. conjugative transfer of a plasmid from a plasmid-bearing to a plasmid-free bacterial cell requires contact and attachment of the cells followed by plasmid dna transfer prior to detachment. we introduce a system of differential equations for plasmid transfer in well-mixed populations that accounts for attachment, dna transfer, and detachment dynamics. ... | 2009 | 19835890 |
| accounting for mating pair formation in plasmid population dynamics. | plasmids are important vehicles for horizontal gene transfer and rapid adaptation in bacteria, including the spread of antibiotic resistance genes. conjugative transfer of a plasmid from a plasmid-bearing to a plasmid-free bacterial cell requires contact and attachment of the cells followed by plasmid dna transfer prior to detachment. we introduce a system of differential equations for plasmid transfer in well-mixed populations that accounts for attachment, dna transfer, and detachment dynamics. ... | 2009 | 19835890 |
| cholesterol oxidase: physiological functions. | an important aspect of catalysis performed by cholesterol oxidase (3beta-hydroxysteroid oxidase) concerns the nature of its association with the lipid bilayer that contains the sterol substrate. efficient catalytic turnover is affected by the association of the protein with the membrane as well as the solubility of the substrate in the lipid bilayer. in this review, the binding of cholesterol oxidase to the lipid bilayer, its turnover of substrates presented in different physical environments, a ... | 2009 | 19843168 |
| metagenomic approaches to natural products from free-living and symbiotic organisms. | 2009 | 19844642 | |
| sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids. | the plasma membrane (pm) is a major resource for production of bioactive lipids and contains a large proportion of the cellular sphingomyelin (sm) content. consequently, the regulation of sm levels at the pm by enzymes such as sphingomyelinase (smase) and sm synthase 2 (sms2) can have profound effects - both on biophysical properties of the membrane, but also on cellular signaling. over the past 20 years, there has been considerable research into the physiological and cellular functions associat ... | 2009 | 19857494 |
| sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids. | the plasma membrane (pm) is a major resource for production of bioactive lipids and contains a large proportion of the cellular sphingomyelin (sm) content. consequently, the regulation of sm levels at the pm by enzymes such as sphingomyelinase (smase) and sm synthase 2 (sms2) can have profound effects - both on biophysical properties of the membrane, but also on cellular signaling. over the past 20 years, there has been considerable research into the physiological and cellular functions associat ... | 2009 | 19857494 |
| function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase pa5185 from pseudomonas aeruginosa pao1. | the crystal structure of pa5185, a putative thioesterase from pseudomonas aeruginosa strain pao1, was solved using multi-wavelength anomalous diffraction to 2.4 a. analysis of the structure and information about the putative function of the protein were used to optimize crystallization conditions. the crystal growth was optimized by applying additives with chemical similarity to a fragment of a putative pa5185 substrate (coa or its derivative). using new crystallization conditions containing thi ... | 2008 | 19898606 |
| the vaginal bacterial communities of japanese women resemble those of women in other racial groups. | to determine whether different racial groups shared common types of vaginal microbiota, we characterized the composition and structure of vaginal bacterial communities in asymptomatic and apparently healthy japanese women in tokyo, japan, and compared them with those of white and black women from north america. the composition of vaginal communities was compared based on community profiles of terminal restriction fragments of 16s rrna genes and phylogenetic analysis of cloned 16s rrna gene seque ... | 2010 | 19912342 |
| the vaginal bacterial communities of japanese women resemble those of women in other racial groups. | to determine whether different racial groups shared common types of vaginal microbiota, we characterized the composition and structure of vaginal bacterial communities in asymptomatic and apparently healthy japanese women in tokyo, japan, and compared them with those of white and black women from north america. the composition of vaginal communities was compared based on community profiles of terminal restriction fragments of 16s rrna genes and phylogenetic analysis of cloned 16s rrna gene seque ... | 2010 | 19912342 |
| novel insights about class 2 integrons from experimental and genomic epidemiology. | in order to contribute to the knowledge of the architecture and epidemiology of class 2 integrons, we performed a class 2 integron molecular survey in which we analyzed 726 isolates in two bacterial populations from environmental and nonepidemiologically related clinical samples, respectively, collected from 1982 to 2007. we recovered the inti2 gene from 130 of 726 isolates, most of which were clinical isolates, and only 1 (a psychrophilic pseudomonas sp.) was from a water sample. unlike the wid ... | 2010 | 19917745 |
| early and late onset sepsis in late preterm infants. | preterm birth is increasing worldwide, and late preterm births, which comprise more than 70% of all preterm births, account for much of the increase. early and late onset sepsis results in significant mortality in extremely preterm infants, but little is known about sepsis outcomes in late preterm infants. | 2009 | 19953725 |
| studies on the mechanism of p-hydroxyphenylacetate 3-hydroxylase from pseudomonas aeruginosa: a system composed of a small flavin reductase and a large flavin-dependent oxygenase. | there are two known types of microbial two-component flavin-dependent monooxygenases that catalyze oxygenation of p-hydroxyphenylacetate (hpa), and they are distinguished by having structurally distinct reductases and oxygenases. this paper presents a detailed analysis of the properties of the enzyme from pseudomonas aeruginosa, an example of one group, and compares its properties to those published for the acinetobacter baumannii enzyme, an example of the alternative group. the reductase and ox ... | 2010 | 20000468 |
| antifungal activity of selected indigenous pseudomonas and bacillus from the soybean rhizosphere. | the purpose of this study was to isolate and select indigenous soil pseudomonas and bacillus bacteria capable of developing multiple mechanisms of action related to the biocontrol of phytopathogenic fungi affecting soybean crops. the screening procedure consisted of antagonism tests against a panel of phytopathogenic fungi, taxonomic identification, detection by pcr of several genes related to antifungal activity, in vitro detection of the antifungal products, and root colonization assays. two i ... | 2009 | 20016811 |
| electron transfer in the complex of membrane-bound human cytochrome p450 3a4 with the flavin domain of p450bm-3: the effect of oligomerization of the heme protein and intermittent modulation of the spin equilibrium. | we studied the kinetics of nadph-dependent reduction of human cyp3a4 incorporated into nanodiscs (cyp3a4-nd) and proteoliposomes in order to probe the effect of p450 oligomerization on its reduction. the flavin domain of cytochrome p450-bm3 (bmr) was used as a model electron donor partner. unlike cyp3a4 oligomers, where only 50% of the enzyme was shown to be reducible by bmr, cyp3a4-nd could be reduced almost completely. high reducibility was also observed in proteoliposomes with a high lipid-to ... | 2010 | 20026040 |
| electron transfer in the complex of membrane-bound human cytochrome p450 3a4 with the flavin domain of p450bm-3: the effect of oligomerization of the heme protein and intermittent modulation of the spin equilibrium. | we studied the kinetics of nadph-dependent reduction of human cyp3a4 incorporated into nanodiscs (cyp3a4-nd) and proteoliposomes in order to probe the effect of p450 oligomerization on its reduction. the flavin domain of cytochrome p450-bm3 (bmr) was used as a model electron donor partner. unlike cyp3a4 oligomers, where only 50% of the enzyme was shown to be reducible by bmr, cyp3a4-nd could be reduced almost completely. high reducibility was also observed in proteoliposomes with a high lipid-to ... | 2010 | 20026040 |
| unique extracellular matrix heparan sulfate from the bivalve nodipecten nodosus (linnaeus, 1758) safely inhibits arterial thrombosis after photochemically induced endothelial lesion. | heparin-like glycans with diverse disaccharide composition and high anticoagulant activity have been described in several families of marine mollusks. the present work focused on the structural characterization of a new heparan sulfate (hs)-like polymer isolated from the mollusk nodipecten nodosus (linnaeus, 1758) and on its anticoagulant and antithrombotic properties. total glycans were extracted from the mollusk and fractionated by ethanol precipitation. the main component (>90%) was identifie ... | 2010 | 20053999 |
| structure and ligand binding properties of the epoxidase component of styrene monooxygenase . | styrene monooxygenase (smo) is a two-component flavoprotein monooxygenase that transforms styrene to styrene oxide in the first step of the styrene catabolic and detoxification pathway of pseudomonas putida s12. the crystal structure of the n-terminally histidine-tagged epoxidase component of this system, nsmoa, determined to 2.3 a resolution, indicates the enzyme exists as a homodimer in which each monomer forms two distinct domains. the overall architecture is most similar to that of p-hydroxy ... | 2010 | 20055497 |
| new structural and functional defects in polyphosphate deficient bacteria: a cellular and proteomic study. | inorganic polyphosphate (polyp), a polymer of tens or hundreds of phosphate residues linked by atp-like bonds, is found in all organisms and performs a wide variety of functions. polyp is synthesized in bacterial cells by the actions of polyphosphate kinases (ppk1 and ppk2) and degraded by exopolyphosphatase (ppx). bacterial cells with polyp deficiencies due to knocking out the ppk1 gene are affected in many structural and important cellular functions such as motility, quorum sensing, biofilm fo ... | 2010 | 20067623 |
| an in vitro spectroscopic analysis to determine whether para-chloroaniline is produced from mixing sodium hypochlorite and chlorhexidine. | the purpose of this in vitro study was to determine whether para-chloroaniline (pca) is formed through the reaction of mixing sodium hypochlorite (naocl) and chlorhexidine (chx). | 2009 | 20113799 |
| an in vitro spectroscopic analysis to determine whether para-chloroaniline is produced from mixing sodium hypochlorite and chlorhexidine. | the purpose of this in vitro study was to determine whether para-chloroaniline (pca) is formed through the reaction of mixing sodium hypochlorite (naocl) and chlorhexidine (chx). | 2009 | 20113799 |
| risk factors and prevention of late-onset sepsis in premature infants. | late-onset sepsis in premature infants is a major cause of morbidity, mortality, and increased medical costs. risk factors include low birth weight, low gestational age, previous antimicrobial exposure, poor hand hygiene, and central venous catheters. methods studied to prevent late-onset sepsis include early feedings, immune globulin administration, prophylactic antimicrobial administration, and improved hand hygiene. in this review, we will outline the risk factors for development of late-onse ... | 2010 | 20116186 |
| structural basis for catalysis by the mono- and dimetalated forms of the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase. | biosynthesis of lysine and meso-diaminopimelic acid in bacteria provides essential components for protein synthesis and construction of the bacterial peptidoglycan cell wall. the dape operon enzymes synthesize both meso-diaminopimelic acid and lysine and, therefore, represent potential targets for novel antibacterials. the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase functions in a late step of the pathway and converts n-succinyl-l,l-diaminopimelic acid to l,l-diaminopimelic aci ... | 2010 | 20138056 |
| mycobacterium tuberculosis nucleoid-associated dna-binding protein h-ns binds with high-affinity to the holliday junction and inhibits strand exchange promoted by reca protein. | a number of studies have shown that the structure and composition of bacterial nucleoid influences many a processes related to dna metabolism. the nucleoid-associated proteins modulate not only the dna conformation but also regulate the dna metabolic processes such as replication, recombination, repair and transcription. understanding of how these processes occur in the context of mycobacterium tuberculosis nucleoid is of considerable medical importance because the nucleoid structure may be cons ... | 2010 | 20176569 |
| managing corneal disease: focus on suppurative keratitis. | 2009 | 20212926 | |
| taking a corneal scrape and making a diagnosis. | 2009 | 20212927 | |
| anthranilate-activating modules from fungal nonribosomal peptide assembly lines. | fungal natural products containing benzodiazepinone- and quinazolinone-fused ring systems can be assembled by nonribosomal peptide synthetases (nrps) using the conformationally restricted beta-amino acid anthranilate as one of the key building blocks. we validated that the first module of the acetylaszonalenin synthetase of neosartorya fischeri nrrl 181 activates anthranilate to anthranilyl-amp. with this as a starting point, we then used bioinformatic predictions about fungal adenylation domain ... | 2010 | 20225828 |
| annual variations in the diversity, viability, and origin of airborne bacteria. | the presence of bacteria in aerosols has been known for centuries, but information on their identity and role in dispersing microbial traits is still limited. this study monitored the airborne bacterial community during an annual survey using samples collected from a 25-m tower near the baltic sea coast. the number of cfu was estimated using agar plates while the most probable number (mpn) of viable bacteria was estimated using dilution-to-extinction culturing assays (dcas). the mpn and cfu valu ... | 2010 | 20228096 |
| a novel hydrolytic dehalogenase for the chlorinated aromatic compound chlorothalonil. | dehalogenases play key roles in the detoxification of halogenated aromatics. interestingly, only one hydrolytic dehalogenase for halogenated aromatics, 4-chlorobenzoyl-coenzyme a (coa) dehalogenase, has been reported. here, we characterize another novel hydrolytic dehalogenase for a halogenated aromatic compound from the 2,4,5,6-tetrachloroisophthalonitrile (chlorothalonil)-degrading strain of pseudomonas sp. ctn-3, which we have named chd. chd catalyzes a hydroxyl substitution at the 4-chlorine ... | 2010 | 20363940 |
| bacterial community dynamics during the application of a myxococcus xanthus-inoculated culture medium used for consolidation of ornamental limestone. | in this study, we investigated under laboratory conditions the bacterial communities inhabiting quarry and decayed ornamental carbonate stones before and after the application of a myxococcus xanthus-inoculated culture medium used for consolidation of the stones. the dynamics of the community structure and the prevalence of the inoculated bacterium, m. xanthus, were monitored during the time course of the consolidation treatment (30 days). for this purpose, we selected a molecular strategy combi ... | 2010 | 20393845 |
| phenolic acids act as signaling molecules in plant-microbe symbioses. | phenolic acids are the main polyphenols made by plants. these compounds have diverse functions and are immensely important in plant-microbe interactions/symbiosis. phenolic compounds act as signaling molecules in the initiation of legumerhizobia symbioses, establishment of arbuscular mycorrhizal symbioses and can act as agents in plant defense. flavonoids are a diverse class of polyphenolic compounds that have received considerable attention as signaling molecules involved in plant-microbe inter ... | 2010 | 20400851 |
| hydroxylated metabolites of 4-monochlorobiphenyl and its metabolic pathway in whole poplar plants. | 4-monochlorobiphenyl (cb3), mainly an airborne pollutant, undergoes rapid biotransformation to produce hydroxylated metabolites (oh-cb3s). however, up to now, hydroxylation of cb3 has not been studied in living organisms. in order to explore the formation of hydroxylated metabolites of cb3 in whole plants, poplars (populus deltoides x nigra, dn34) were exposed to cb3 for 10 days. poplars are a model plant with complete genomic sequence, and they are widely used in phytoremediation. results showe ... | 2010 | 20402517 |
| bacteriological study of aerobic isolates from plantar ulcers of paucibacillary leprosy patients. | plantar ulcers commonly occur in leprosy patients, which usually recur and cause morbidity in such cases. | 2010 | 20418976 |
| expression, purification, crystallization and preliminary x-ray analysis of pseudomonas aeruginosa algx. | algx is a periplasmic protein required for the production of the exopolysaccharide alginate in pseudomonas sp. and azotobacter vinelandii. algx has been overexpressed and purified and diffraction-quality crystals have been grown using iterative seeding and the hanging-drop vapor-diffusion method. the crystals grew as flat plates with unit-cell parameters a = 46.4, b = 120.6, c = 86.9 a, beta = 95.7 degrees . the crystals exhibited the symmetry of space group p2(1) and diffracted to a minimum d-s ... | 2010 | 20445266 |
| kinetic and structural characterization of a heterohexamer 4-oxalocrotonate tautomerase from chloroflexus aurantiacus j-10-fl: implications for functional and structural diversity in the tautomerase superfamily . | 4-oxalocrotonate tautomerase (4-ot) isozymes play prominent roles in the bacterial utilization of aromatic hydrocarbons as sole carbon sources. these enzymes catalyze the conversion of 2-hydroxy-2,4-hexadienedioate (or 2-hydroxymuconate) to 2-oxo-3-hexenedioate, where pro-1 functions as a general base and shuttles a proton from the 2-hydroxyl group of the substrate to the c-5 position of the product. 4-ot, a homohexamer from pseudomonas putida mt-2, is the most extensively studied 4-ot isozyme a ... | 2010 | 20465238 |
| incidence, relevance and response for ralsfonia respiratory infections. | cases of ralstonia colonization/infection occasionally reported by hospitals has generated increased interest in an organism previously little known to most clinicians. our goal was to determine the incidence of respiratory colonizations and infections involving ralstonia and the association of mechanical ventilation (limited to reports on respiratory-related occurrences in the u.s.a.) and propose a decision chart to assist response. | 2010 | 20499534 |
| thioesterases: a new perspective based on their primary and tertiary structures. | thioesterases (tes) are classified into ec 3.1.2.1 through ec 3.1.2.27 based on their activities on different substrates, with many remaining unclassified (ec 3.1.2.-). analysis of primary and tertiary structures of known tes casts a new light on this enzyme group. we used strong primary sequence conservation based on experimentally proved proteins as the main criterion, followed by verification with tertiary structure superpositions, mechanisms, and catalytic residue positions, to accurately de ... | 2010 | 20506386 |
| macrocyclic histone deacetylase inhibitors. | histone deacetylase inhibitors (hdaci) are an emerging class of novel anti-cancer drugs that cause growth arrest, differentiation, and apoptosis of tumor cells. in addition, they have shown promise as anti-parasitic, anti-neurodegenerative, anti-rheumatologic and immunosuppressant agents. to date, several structurally distinct small molecule hdaci have been reported including aryl hydroxamates, benzamides, short-chain fatty acids, electrophilic ketones, and macrocyclic peptides. macrocyclic hdac ... | 2010 | 20536416 |
| molecular characterization of a class i p450 electron transfer system from novosphingobium aromaticivorans dsm12444. | cytochrome p450 (cyp) enzymes of the cyp101 and cyp111 families from the oligotrophic bacterium novosphingobium aromaticivorans dsm12444 are heme monooxygenases that receive electrons from nadh via arx, a [2fe-2s] ferredoxin, and arr, a ferredoxin reductase. these systems show fast nadh turnovers (k(cat) = 39-91 s(-1)) that are efficiently coupled to product formation. the three-dimensional structures of arr, arx, and cyp101d1, which form a physiological class i p450 electron transfer chain, hav ... | 2010 | 20576606 |
| identification and characterization of a novel estrogenic ligand actinopolymorphol a. | xenoestrogenic compounds are abundant in the modern environment including phytoestrogens from plants, chemical by-products from industry, and secondary metabolites from microbes; all can profoundly affect human health. consequently mechanism-based screens are urgently needed to improve the rate at which the xenoestrogens are discovered. estrogen receptor (er) dimerization is required for target gene transcription. the three er dimer pairs (eralpha/alpha homodimers, erbeta/beta homodimers, and er ... | 2010 | 20599778 |
| favored and disfavored pathways of protein crosslinking by glucose: glucose lysine dimer (glucold) and crossline versus glucosepane. | we describe the isolation and molecular characterization of a novel glucose-lysine dimer crosslink 1,3-bis-(5-amino-5-carboxypentyl)-4-(1',2',3',4'-tetrahydroxybutyl)-3h-imidazolium salt, named glucold. glucold was easily formed from the amadori product (fructose-lysine). however, when bsa was incubated with 100 mm glucose for 25 days, the levels of the lysine-lysine glucose crosslinks glucold and crossline were only 21 and <1 pmol/mg, respectively, compared to 611 pmol/mg protein for the lysine ... | 2010 | 20607325 |
| favored and disfavored pathways of protein crosslinking by glucose: glucose lysine dimer (glucold) and crossline versus glucosepane. | we describe the isolation and molecular characterization of a novel glucose-lysine dimer crosslink 1,3-bis-(5-amino-5-carboxypentyl)-4-(1',2',3',4'-tetrahydroxybutyl)-3h-imidazolium salt, named glucold. glucold was easily formed from the amadori product (fructose-lysine). however, when bsa was incubated with 100 mm glucose for 25 days, the levels of the lysine-lysine glucose crosslinks glucold and crossline were only 21 and <1 pmol/mg, respectively, compared to 611 pmol/mg protein for the lysine ... | 2010 | 20607325 |
| innate immunology of bovine respiratory disease. | there are a variety of factors and conditions that predispose cattle to pneumonia. cattle have anatomic and cellular differences from humans and other species and are managed in groups/herds, all of which can increase susceptibility to microbial pathogens. this article highlights the basic innate immune response of the respiratory tract and newer developments in the understanding of adaptive immune responses of the bovine respiratory tract, placing special emphasis on features unique to cattle. | 2010 | 20619180 |
| stya1 and stya2b from rhodococcus opacus 1cp: a multifunctional styrene monooxygenase system. | two-component flavoprotein monooxygenases are emerging biocatalysts that generally consist of a monooxygenase and a reductase component. here we show that rhodococcus opacus 1cp encodes a multifunctional enantioselective flavoprotein monooxygenase system composed of a single styrene monooxygenase (smo) (stya1) and another styrene monooxygenase fused to an nadh-flavin oxidoreductase (stya2b). stya1 and stya2b convert styrene and chemical analogues to the corresponding epoxides at the expense of f ... | 2010 | 20675468 |
| scarless and sequential gene modification in pseudomonas using pcr product flanked by short homology regions. | the lambda red recombination system has been used to inactivate chromosomal genes in various bacteria and fungi. the procedure consists of electroporating a polymerase chain reaction (pcr) fragment containing antibiotic cassette flanked by homology regions to the target locus into a strain that can express the lambda red proteins (gam, bet, exo). | 2010 | 20682065 |
| single turnover kinetics of tryptophan hydroxylase: evidence for a new intermediate in the reaction of the aromatic amino acid hydroxylases. | tryptophan hydroxylase (trph) uses a non-heme mononuclear iron center to catalyze the tetrahydropterin-dependent hydroxylation of tryptophan to 5-hydroxytryptophan. the reactions of the trph.fe(ii), trph.fe(ii).tryptophan, trph.fe(ii).6meph(4).tryptophan, and trph.fe(ii).6meph(4).phenylalanine complexes with o(2) were monitored by stopped-flow absorbance spectroscopy and rapid quench methods. the second-order rate constant for the oxidation of trph.fe(ii) has a value of 104 m(-1) s(-1) irrespect ... | 2010 | 20687613 |
| moenomycin family antibiotics: chemical synthesis, biosynthesis, and biological activity. | 2010 | 20730219 | |
| partial purification and characterization of the mode of action of enterocin s37: a bacteriocin produced by enterococcus faecalis s37 isolated from poultry feces. | the aim of this research was to purify and characterize the mode of action of enterocin s37, a bacteriocin produced by enterococcus faecalis s37, a strain recently isolated from the chicken feces. enterocin s37 has a molecular weight comprised between 4 and 5 kda. it remained active after 1 h at 80(o)c and at ph values ranging from 4.0 to 9.0. furthermore, cell-free supernatant of enterococcus faecalis s37 and purified enterocin s37 were active against gram-positive bacteria including listeria m ... | 2010 | 20811593 |
| functional gene diversity of soil microbial communities from five oil-contaminated fields in china. | to compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of china. geochip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. our results indicated that the overall microbial com ... | 2010 | 20861922 |
| functional gene diversity of soil microbial communities from five oil-contaminated fields in china. | to compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of china. geochip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. our results indicated that the overall microbial com ... | 2010 | 20861922 |
| isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. | bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ... | 2010 | 20874394 |
| lipoic acid synthesis: a new family of octanoyltransferases generally annotated as lipoate protein ligases. | bacillus subtilis lacks a recognizable homologue of the lipb octanoyltransferase, an enzyme essential for lipoic acid synthesis in escherichia coli. lipb transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-lipb intermediate. the octanoylated dehydrogenase is then converted to the enzymatically active lipoylated species by insertion of two sulfur atoms into the octanoyl moiety by the s-adenosyl-l-me ... | 2010 | 20882995 |
| activity and interactions of antibiotic and phytochemical combinations against pseudomonas aeruginosa in vitro. | in this study the in vitro activities of seven antibiotics (ciprofloxacin, ceftazidime, tetracycline, trimethoprim, sulfamethoxazole, polymyxin b and piperacillin) and six phytochemicals (protocatechuic acid, gallic acid, ellagic acid, rutin, berberine and myricetin) against five p. aeruginosa isolates, alone and in combination are evaluated. all the phytochemicals under investigation demonstrate potential inhibitory activity against p. aeruginosa. the combinations of sulfamethoxazole plus proto ... | 2010 | 20941374 |
| prediction and analysis of the modular structure of cytochrome p450 monooxygenases. | cytochrome p450 monooxygenases (cyps) form a vast and diverse family of highly variable sequences. they catalyze a wide variety of oxidative reactions and are therefore of great relevance in drug development and biotechnological applications. despite their differences in sequence and substrate specificity, the structures of cyps are highly similar. although being in research focus for years, factors mediating selectivity and activity remain vague. | 2010 | 20950472 |
| expression of an organic solvent stable lipase from staphylococcus epidermidis at2. | an organic solvent tolerant lipase gene from staphylococcus epidermidis at2 was successfully cloned and expressed with ptrchis2 in e. coli top10. sequence analysis revealed an open reading frame (orf) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. the polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids. expression of at2 lipase resulted in an 18-fold increase in activity, upon the induction of 0.6 mm ipt ... | 2010 | 20957088 |
| gene duplications in prokaryotes can be associated with environmental adaptation. | gene duplication is a normal evolutionary process. if there is no selective advantage in keeping the duplicated gene, it is usually reduced to a pseudogene and disappears from the genome. however, some paralogs are retained. these gene products are likely to be beneficial to the organism, e.g. in adaptation to new environmental conditions. the aim of our analysis is to investigate the properties of paralog-forming genes in prokaryotes, and to analyse the role of these retained paralogs by relati ... | 2010 | 20961426 |
| functional promiscuity of homologues of the bacterial arsa atpases. | the arsa atpase of e. coli plays an essential role in arsenic detoxification. published evidence implicates arsa in the energization of as(iii) efflux via the formation of an oxyanion-translocating complex with arsb. in addition, eukaryotic arsa homologues have several recognized functions unrelated to arsenic resistance. by aligning arsa homologues, constructing phylogenetic trees, examining arsa encoding operons, and estimating the probable coevolution of these homologues with putative transpo ... | 2010 | 20981284 |
| keratitis after implantation of intrastromal corneal rings with spontaneous extrusion of the segment. | purpose: to report a case of bacterial keratitis in a patient with a history of intrastromal corneal ring segments (intacs®) implantation to correct keratoconus. methods: the patient's history, clinical presentation, pathological analysis and therapeutic management were reviewed. results: a 36-year-old-man was referred to our department due to decreased vision and intense pain in his left eye, 40 days after intacs® implantation for keratoconus. slit-lamp examination revealed epithelial defects a ... | 2010 | 21060771 |
| thny is a ferredoxin reductase-like iron-sulfur flavoprotein that has evolved to function as a regulator of tetralin biodegradation gene expression. | previous genetic studies in sphingomonas macrogolitabida strain tfa have established that expression of genes involved in tetralin biodegradation (thn genes) requires the function of the lysr type activator thnr and also thny. sequence comparison indicated that thny is homologous to bacterial oxygenase-coupled nad(p)h-dependent ferredoxin reductases. however, thny showed substitutions in highly conserved positions of the pyridine nucleotide binding domain of these ferredoxin reductases. thny exp ... | 2010 | 21068394 |
| thny is a ferredoxin reductase-like iron-sulfur flavoprotein that has evolved to function as a regulator of tetralin biodegradation gene expression. | previous genetic studies in sphingomonas macrogolitabida strain tfa have established that expression of genes involved in tetralin biodegradation (thn genes) requires the function of the lysr type activator thnr and also thny. sequence comparison indicated that thny is homologous to bacterial oxygenase-coupled nad(p)h-dependent ferredoxin reductases. however, thny showed substitutions in highly conserved positions of the pyridine nucleotide binding domain of these ferredoxin reductases. thny exp ... | 2010 | 21068394 |
| structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. | the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ... | 2010 | 21073881 |
| structural insight into the expanded pcb-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. | the biphenyl dioxygenase of burkholderia xenovorans lb400 is a multicomponent rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (pcbs). the structural bases for the substrate specificity of the enzyme's oxygenase component (bphae(lb400)) are largely unknown. bphae(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls, including 2,6-dichlorobiphenyl, more efficiently than bphae(lb400), yet differs fr ... | 2010 | 21073881 |
| hydrogenosome-localization of arginine deiminase in trichomonas vaginalis. | the arginine dihydrolase (adh) pathway has an analogous function to the urea cycle in mitochondria-containing cells, by removing nitrogen from amino acids and generating atp. subcellular localization of the adh pathway enzymes in trichomonas vaginalis revealed that arginine deiminase (adi) localizes to the hydrogenosome, a mitochondrion-like organelle of anaerobic protists. however the other enzymes of the adh pathway, ornithine carbamyltransferase and carbamate kinase localize to the cytosol. t ... | 2010 | 21074581 |
| hydrogenosome-localization of arginine deiminase in trichomonas vaginalis. | the arginine dihydrolase (adh) pathway has an analogous function to the urea cycle in mitochondria-containing cells, by removing nitrogen from amino acids and generating atp. subcellular localization of the adh pathway enzymes in trichomonas vaginalis revealed that arginine deiminase (adi) localizes to the hydrogenosome, a mitochondrion-like organelle of anaerobic protists. however the other enzymes of the adh pathway, ornithine carbamyltransferase and carbamate kinase localize to the cytosol. t ... | 2010 | 21074581 |
| giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosine ligase-like member and deglycylated by two metallocarboxypeptidases. | the flagellated protozoan giardia duodenalis is a parasite of the upper part of the small intestine of mammals, including humans, and an interesting biological model. giardia harbors a single 14-3-3 isoform, a multifunctional protein family, that is modified at the c terminus by polyglycylation, an unusual post-translational modification consisting of the covalent addition of one or multiple glycines on the γ-carboxyl groups of specific glutamic acids. polyglycylation affects the intracellular l ... | 2010 | 21135098 |
| giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosine ligase-like member and deglycylated by two metallocarboxypeptidases. | the flagellated protozoan giardia duodenalis is a parasite of the upper part of the small intestine of mammals, including humans, and an interesting biological model. giardia harbors a single 14-3-3 isoform, a multifunctional protein family, that is modified at the c terminus by polyglycylation, an unusual post-translational modification consisting of the covalent addition of one or multiple glycines on the γ-carboxyl groups of specific glutamic acids. polyglycylation affects the intracellular l ... | 2010 | 21135098 |
| nature of the reaction intermediates in the flavin adenine dinucleotide-dependent epoxidation mechanism of styrene monooxygenase. | styrene monooxygenase (smo) is a two-component flavoenzyme composed of an nadh-specific flavin reductase (smob) and fad-specific styrene epoxidase (nsmoa). nsmoa binds tightly to reduced fad and catalyzes the stereospecific addition of one atom of molecular oxygen to the vinyl side chain of styrene in the enantioselective synthesis of s-styrene oxide. in this mechanism, molecular oxygen first reacts with nsmoa(fad(red)) to yield an fad c(4a)-peroxide intermediate. this species is nonfluorescent ... | 2010 | 21166448 |
| nature of the reaction intermediates in the flavin adenine dinucleotide-dependent epoxidation mechanism of styrene monooxygenase. | styrene monooxygenase (smo) is a two-component flavoenzyme composed of an nadh-specific flavin reductase (smob) and fad-specific styrene epoxidase (nsmoa). nsmoa binds tightly to reduced fad and catalyzes the stereospecific addition of one atom of molecular oxygen to the vinyl side chain of styrene in the enantioselective synthesis of s-styrene oxide. in this mechanism, molecular oxygen first reacts with nsmoa(fad(red)) to yield an fad c(4a)-peroxide intermediate. this species is nonfluorescent ... | 2010 | 21166448 |
| screening of ethanol, petroleum ether and chloroform extracts of medicinal plants, lawsonia inermis l. and mimosa pudica l. for antibacterial activity. | organic extracts (ethanol, petroleum ether and chloroform) of two medicinal plants lawsonia inermis l. and mimosa pudica l. were proven for antibacterial properties against 15 gram-positive and gram-negative human pathogenic bacteria. among the three types of extracts tested, ethanol extract was found to possess maximum antibacterial activity. the diameter of the zone of inhibition of bacterial growth showed that gram-negative bacteria are more sensitive than gram-positive bacteria to plant extr ... | 2010 | 21188055 |
| real-time solvent tolerance analysis of pseudomonas sp. strain vlb120{delta}c catalytic biofilms. | biofilms are ubiquitous surface-associated microbial communities embedded in an extracellular polymeric (eps) matrix, which gives the biofilm structural integrity and strength. it is often reported that biofilm-grown cells exhibit enhanced tolerance toward adverse environmental stress conditions, and thus there has been a growing interest in recent years to use biofilms for biotechnological applications. we present a time- and locus-resolved, noninvasive, quantitative approach to study biofilm d ... | 2010 | 21193676 |
| wses consensus conference: guidelines for first-line management of intra-abdominal infections. | intra-abdominal infections are still associated with high rate of morbidity and mortality.a multidisciplinary approach to the management of patients with intra-abdominal infections may be an important factor in the quality of care. the presence of a team of health professionals from various disciplines, working in concert, may improve efficiency, outcome, and the cost of care.a world society of emergency surgery (wses) consensus conference was held in bologna on july 2010, during the 1st congres ... | 2011 | 21232143 |
| mechanistic studies of 1-aminocyclopropane-1-carboxylate deaminase: characterization of an unusual pyridoxal 5'-phosphate-dependent reaction. | 1-aminocyclopropane-1-carboxylic acid (acc) deaminase (accd) is a pyridoxal 5'-phosphate (plp)-dependent enzyme that cleaves the cyclopropane ring of acc, to give α-ketobutyric acid and ammonia as products. the cleavage of the c(α)-c(β) bond of an amino acid substrate is a rare event in plp-dependent enzyme catalysis. potential chemical mechanisms involving nucleophile- or acid-catalyzed cyclopropane ring opening have been proposed for the unusual transformation catalyzed by accd, but the actual ... | 2011 | 21244019 |
| aldehyde oxidase carrying an unusual subunit structure from pseudomonas sp. mx-058. | pseudomonas sp. mx-058 produces aldehyde oxidase catalysing glyoxal to glyoxylic acid. two aldehyde oxidases (f10 and f13) were purified to homogeneity from pseudomonas sp. mx-058. f10 and f13 had subunit structures, a heterotetramer and heteropentamer respectively. the n-terminal amino acid sequences of all subunits were highly homologous to amino acid sequences of the putative oxidoreductases of pseudomonas strains. all of these homologous oxidoreductases have a heterotrimer structure consisti ... | 2008 | 21261859 |
| atrazine biodegradation in the lab and in the field: enzymatic activities and gene regulation. | atrazine is an herbicide of the s-triazine family that is used primarily as a nitrogen source by degrading microorganisms. while many catabolic pathways for xenobiotics are subjected to catabolic repression by preferential carbon sources, atrazine utilization is repressed in the presence of preferential nitrogen sources. this phenomenon appears to restrict atrazine elimination in nitrogen-fertilized soils by indigenous organisms or in bioaugmentation approaches. the mechanisms of nitrogen contro ... | 2009 | 21261912 |
| differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynx. | 2011 | 21261963 | |
| species sorting and neutral processes are both important during the initial assembly of bacterial communities. | many studies have shown that species sorting, that is, the selection by local environmental conditions is important for the composition and assembly of bacterial communities. on the other hand, there are other studies that could show that bacterial communities are neutrally assembled. in this study, we implemented a microcosm experiment with the aim to determine, at the same time, the importance of species sorting and neutral processes for bacterial community assembly during the colonisation of ... | 2011 | 21270841 |
| clinical and antimicrobial efficacy of nitradine ™-based disinfecting cleaning tablets in complete denture wearers. | this study evaluated the efficacy of nitradine ™-based disinfecting cleaning tablets for complete denture, in terms of denture biofilm removal and antimicrobial action. | 2010 | 21308285 |
| engineering of a psychrophilic bacterium for the bioremediation of aromatic compounds. | microbial degradation of aromatic hydrocarbons has been studied with the aim of developing applications for the removal of toxic compounds. efforts have been directed toward the genetic manipulation of mesophilic bacteria to improve their ability to degrade pollutants, even though many pollution problems occur in sea waters and in effluents of industrial processes which are characterized by low temperatures. from these considerations the idea of engineering a psychrophilic microorganism for the ... | 2010 | 21326928 |
| recombinant organisms for production of industrial products. | a revolution in industrial microbiology was sparked by the discoveries of ther double-stranded structure of dna and the development of recombinant dna technology. traditional industrial microbiology was merged with molecular biology to yield improved recombinant processes for the industrial production of primary and secondary metabolites, protein biopharmaceuticals and industrial enzymes. novel genetic techniques such as metabolic engineering, combinatorial biosynthesis and molecular breeding te ... | 2009 | 21326937 |
| recombinant organisms for production of industrial products. | a revolution in industrial microbiology was sparked by the discoveries of ther double-stranded structure of dna and the development of recombinant dna technology. traditional industrial microbiology was merged with molecular biology to yield improved recombinant processes for the industrial production of primary and secondary metabolites, protein biopharmaceuticals and industrial enzymes. novel genetic techniques such as metabolic engineering, combinatorial biosynthesis and molecular breeding te ... | 2009 | 21326937 |
| microbial degradation of petroleum hydrocarbon contaminants: an overview. | one of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. accidental releases of petroleum products are of particular concern in the environment. hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. ... | 2010 | 21350672 |
| microbial degradation of petroleum hydrocarbon contaminants: an overview. | one of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. accidental releases of petroleum products are of particular concern in the environment. hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. ... | 2010 | 21350672 |