Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [significance of human leptospirosis in mexico. detection of leptospira antibodies in a blood donor population]. | the presence of specific serum antibodies has been used as a diagnostic test for human leptospirosis. the presence of these antibodies in humans is indicative of an active natural infection. its detection after exposure denotes the presence of immunity. serum samples from 206 adult blood donors were analyzed with a microscopic agglutination assay against 7 serovars of leptospira interrogans. a total of 7% were positive with the following serovar distribution; shermani 53%, canicola 33%, pyrogens ... | 1995 | 8582567 |
| [seroepidemiologic investigations in the alpine ibex (capra i. ibex) of piz albris in the canton of grigioni (switzerland)]. | sero-epidemiological investigations in wild animals may allow to assess distribution of selected pathogens that sometimes seem to be involved in sanitary interrelationships between wild and domestic ungulates sharing the same areas. serological studies were carried out to investigate the prevalence of antibody against 8 pathogens in alpine ibex of albris colony (grisons, switzerland). investigated sera came from 89 animals shot by gamekeepers in 1990-1991. antibody against smooth brucella, coxie ... | 1995 | 8584868 |
| is1533-based pcr assay for identification of leptospira interrogans sensu lato serovars. | a pcr-based assay was developed for typing l. interrogans sensu lato serovars. the assay is designed to exploit the presence of many copies of the leptospiral insertion sequence is1533 and is1533-like sequences present in the genomes of most leptospiral serovars. the pcr primers were designed to amplify dna of unknown sequence between closely placed is1533 or is1533-like sequences. amplification reactions primed with is1533-based primers generated products of different sizes. when few copies of ... | 1995 | 8586718 |
| [leptospira infected rat population as probable cause of a fatal case of weil's disease]. | in the summer of 1992 a patient died of a leptospiral infection which he probably had contracted while he was swimming in an artificial lake in the region of tübingen/reutlingen. regarding the epidemiological role of leptospirosis the rodent population was investigated, because rats and mice were often seen in the surrounding area. 11 rats and 20 mice could be trapped. from their urine or kidneys two leptospiral serovars were isolated: serovar copenhageni from rattus norvegicus and serovar saxko ... | 1995 | 8593131 |
| leptospira interrogans exposure in free-ranging elk in washington. | exposure to one or more serovars of leptospira interrogans was observed in five of six sampled elk (cervus elaphus roosevelti) killed in november 1993, from an isolated herd in southwest washington, usa (46 degrees 45'n, 123 degrees 6'w). in april 1994, exposure to l. interrogans serovars was documented in nine of 11 captured cow elk from the same herd. leptospires were not isolated from any of the exposed elk, and 10 of the 11 cows were pregnant. the high seroprevalence is evidence that exposur ... | 1996 | 8627923 |
| isolation of leptospira interrogans serovar grippotyphosa from a heifer in new south wales. | 1996 | 8660211 | |
| presence of antigen and antibodies in serum and genital discharges of heifers after experimental intrauterine inoculation with leptospira interrogans serovar hardjo. | the excretion of leptospira interrogans serovar hardjo in cervico-vaginal mucus (cvm) or urine and the local and systemic immune responses to the organism were monitored in eight susceptible heifers after intrauterine inoculation while six similar heifers served as controls. all the heifers were inseminated at the subsequent oestrous periods. the overall percentage pregnancy rate (the number of pregnancies divided by the total number of inseminations) was lower in the infected heifers than in th ... | 1996 | 8685538 |
| presence of antigen and antibodies in serum and genital discharges of cows from dairy herds naturally infected with leptospira interrogans serovar hardjo. | samples of cervico-vaginal mucus from 163 bulling cows (group 1) and post calving discharges from 59 newly calved cows (group 2) in five dairy herds naturally infected with leptospira interrogans serovar hardjo were examined for the presence of antigen and igg and iga antibodies by using two elisa systems which were protein or carbohydrate based. corresponding serum samples were examined for systemic immune responses by using a microscopic agglutination test (mat) and igg-elisa tests. antigen wa ... | 1996 | 8685539 |
| reproductive performance of dairy herds infected with leptospira interrogans serovar hardjo relative to the year of diagnosis. | to assess the impact of leptospira interrogans serovar hardjo infection on the reproductive performance of nine dairy herds with evidence of infection, forty years' fertility data were analysed relative to the year of first diagnosis. fifty per cent of various fertility variables had their lowest values only in the year of diagnosis. culling rates were highest during the year of diagnosis in five of the herds, and were above 22 per cent in five of nine (55-6 per cent) of the diagnosis years cons ... | 1996 | 8711883 |
| leptospirosis in madras--a clinical and serological study. | leptospirosis was confirmed by microscopic agglutination test (mat) and/or elisa in 57 patients admitted to the government general hospital, madras, india, during november and december of 1990 and 1991 with symptomatology suggestive of the disease. fifty (88%) of the 57 cases were males; the mean age of all the cases was 39.6 years (range 17-72). the main clinical features were: fever 100% jaundice 84%, myalgia 82%, acute renal failure 72% and conjunctival suffusion 58%. non-azotemic jaundice oc ... | 1995 | 8713215 |
| preliminary evaluation of antimicrobial agents for treatment of leptospira interrogans serovar pomona infection in hamsters and swine. | to evaluate antimicrobial agents for treatment of models of acute and persistent leptospirosis caused by leptospira interrogans serovar pomona. | 1996 | 8720239 |
| leptospira interrogans serovar hardjo in the kidneys and genital tracts of naturally infected sheep. | a bacteriological study was carried out to identify possible renal and/or genital carriers of leptospira interrogans serovar hardjo. l. hardjo was found at slaughter in the kidneys of three seropositive ewes, but not in uterus or salpinges of these animals. | 1996 | 8722315 |
| effect of vaccination against leptospira interrogans serovar hardjo on milk production and fertility in dairy cattle. | 1996 | 8730678 | |
| [construction of genomic library of l. interrogans serovar lai and preliminary study of recombinant plasmid pdc38]. | a genomic library, consisting of approximate 12 000 recombinants, has been constructed for the leptospira interrogans serovar lai in e. coli. using puc18 as the vector. hybridization analysis with the dna fragment containing ompl1 gene was performed and 10 positive clones were screened from the genomic library. one of the positive clones, designated pdc38, showed hybridization signal with the dna of 7 serovars 8 strains of pathogenic leptospires, but not with the dna of nonpathogenic leptospires ... | 1995 | 8732051 |
| rapid distinction between leptonema and leptospira by pcr amplification of 16s-23s ribosomal dna spacer. | the pcr amplification of the genomic dna of leptonema illini strain 3055 using primers directed against conserved regions of the rrna operon provided evidence that the 16s and 23s rrna genes were linked via an intergenic spacer region. the sequencing of the intergenic spacer region indicated that it was 435 nucleotides in length and sequence similarity searches revealed that it bore no homology to any known sequences including trna available in databases. further investigations using southern bl ... | 1996 | 8759793 |
| intestinal spirochetosis: first cases reported in brazil and the use of immunohistochemistry as an aid in histopathological diagnosis. | colonization of the colon and rectum by intestinal spirochetes is detected for the first time in brazil in 4 of 282 (1.41%) patients who had undergone sigmoidoscopy and/or colonoscopy with a histopathological diagnosis of chronic non specific-colitis. this frequency is probably underestimated, since surgically obtained specimens were not considered in the present study. histopathological diagnosis was performed using routine stains like hematoxylin-eosin which showed the typical, of 3-microns th ... | 1996 | 8762639 |
| [the molecular genetic and biological aspects of the ecology of pathogenic leptospira (leptospira interrogans)]. | the results of recent investigations on the application of some genotyping methods (genomic fingerprinting, analysis of restriction length fragment polymorphism of dna, etc.), made in order to study the population structure of pathogenic leptospires and to evaluate their intraspecies heterogeneity with regard to their main ecological features, are reviewed. new data on the use of prc-based amplification test systems for the study of specific features of host persistence of leptospires are presen ... | 1996 | 8771722 |
| experimental immunization of hamsters with an edta extract of leptospira interrogans, serovar icterohaemorrhagiae. | the edta extract was prepared from the leptospira interrogans serovar icterohaemorrhagiae. when inoculated subcutaneously in hamsters it conferred protection from challenge with virulent leptospires with a low dose (10 micrograms/ml) and low agglutinating antibody (40). the protection of animals obtained by the edta extract opens up the perspective of its use as a component of a vaccine for the control of leptospirosis. | 1996 | 8783904 |
| correlation between dna restriction fragment length polymorphisms in leptospira interrogans serovar pomona type kennewicki and host animal source. | isolates (n = 147) of leptospira interrogans serovar pomona type kennewicki from cattle, swine, horses, and wildlife were analyzed by dna restriction endonuclease analysis. restriction fragment length polymorphisms were identified in dna digested with hpaii, and the restriction fragment length polymorphisms were correlated with the host animal source of the isolates. these results will be useful in understanding the epidemiology of serovar pomona infections in livestock. | 1996 | 8789028 |
| acute renal disease due to leptospira interrogans in a weanling. | 1996 | 8818600 | |
| leptospira interrogans serovar grippotyphosa infection in dogs. | leptospirosis attributed to infection with serovar grippotyphosa was diagnosed in 11 dogs. in naturally and experimentally infected dogs, a stereotypic serologic response to infection with leptospira serovar grippotyphosa was detected. although the highest serum antibody titers developed against serovar grippotyphosa, most dogs also had lower titers against serovars bratislava and pomona. acute renal failure was evident in 10 dogs. one dog died prior to initiation of treatment; the remaining 10 ... | 1996 | 8837647 |
| leptospira interrogans in the genital tract of sheep. research on ewes and rams experimentally infected with serovar hardjo (hardjobovis). | to verify if leptospira hardjo can colonize the male and female genital organs of sheep, 9 animals (6 non pregnant ewes and 3 mature rams) were infected with a strain of l. hardjobovis recently recovered from the kidneys of a seropositive ewe. postinfection controls (bacteriologic, serologic, immunohistochemistry and electron microscopy) failed to disclose the presence of leptospires in the uterus and oviducts, testicles, epididymis, prostate and bulbourethral glands of animals used for the expe ... | 1996 | 8841039 |
| reduced conception rates in dairy cattle associated with serological evidence of leptospira interrogans serovar hardjo infection. | fertility data from 673 cows in five dairy herds with a moderate to high seroprevalence of microscopic agglutination titres (mat) of > or = 1:10 against leptospira interrogans serovar hardjo were collated to assess the relationship between pregnancy rates and antibody titres to serovar hardjo. a significant proportion of failures of conception (10 to 13 per cent, p < 0.001) were associated with mats of 1:10 to 1:100; the overall pregnancy rate of the seronegative cows was 28.5 per cent higher (p ... | 1996 | 8856888 |
| protective activity of rabbit polyclonal anti-idiotype antibody against leptospira interrogans infection in hamsters. | we prepared an anti-idiotype (id) antibody against leptospirosis. serum from rabbit immunized with monoclonal antibody (mab) lw2, which reacted to the main protective antigen prepared from leptospira interrogans serovar lai, inhibited agglutination of the organism by mab lw2. the immune rabbit serum was applied to a column coupled with normal mouse igg as a ligand (first column), and the unbound fraction eluted was applied to a column coupled with mab lw2 as a ligand (second column). the bound f ... | 1996 | 8860969 |
| the development of a ligase mediated pcr with potential for the differentiation of serovars within leptospira interrogans. | a ligase mediated polymerase chain reaction (lmpcr) was developed to amplify between the repetitive element, is1533, of leptospira and adjacent chromosomally located bg/ii restriction endonuclease enzyme sites. to do this, complimentary oligonucleotide linkers designed to anneal together with an overhanging bg/ii end were ligated to bg/ii digested dna from 35 leptospiral reference strains and field isolates. this ligated dna was used as template for pcr with oligonucleotide primers specific for ... | 1996 | 8870196 |
| production and characterization of monoclonal antibodies to the edta extract of leptospira interrogans, serovar icterohaemorrhagiae. | monoclonal antibodies (mabs) were produced against an ethylenediaminetetraacetate (edta) extract of leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as igm and igg (igg1 and igg2b). the edta extract was detected as several bands by silver staining in sds-page. in the western blot the bands around 20 kda reacted with a monoclonal antibody, 47b4d6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohy ... | 1996 | 8885673 |
| effect of leptospira interrogans serovar hardjo infection on milk yield in endemically infected dairy herds. | 1996 | 8893491 | |
| restriction fragment length polymorphisms in australian and new zealand isolates of leptospira interrogans serovar pomona. | 1996 | 8894026 | |
| detection of leptospiral plasmid and comparison of plasmid profiles between virulent and avirulent leptospires. | detection of plasmid from leptospires and a comparison of the plasmid profiles between virulent and avirulent strains were performed to investigate whether leptospires contained plasmid(s) associated with virulence. virulent strains of leptospira interrogans serovars copenhageni, lai, canicola and pomona, which were virulent for the guinea pig and/or hamster and which showed chemotaxis toward hemoglobin, contained approximately 370 kilobases (kb) plasmid. avirulent strains of l. interrogans also ... | 1996 | 8898293 |
| sporadic urban leptospirosis. | surprisingly, many inner-city residents have antibodies to leptospira interrogans. the manner in which these persons acquire this organism in the absence of recognized occupational, recreational, or epidemic risk factors is not known. | 1996 | 8928985 |
| immunochemical characteristics and localization on cells of protective antigen (pag) prepared from leptospira interrogans serovar lai. | immuno-electron microscopic methods revealed that the protective antigen (pag) of leptospira interrogans serovar lai exists on the outer envelope sheathing the leptospiral cell body. pag lost its protective activity after treatment by hydrolysis with 2 m formic acid at 100 c for 2 hr, or oxidation with periodate at 4 c for 40 hr. the antigenic oligosaccharide fraction was further purified from the hydrolyzed pag by immunoaffinity column coupled with protective monoclonal antibody, lw2, and by ge ... | 1996 | 8934679 |
| rapid identification of pathogenic leptospira species (leptospira interrogans, l. borgpetersenii, and l. kirschneri) with species-specific dna probes produced by arbitrarily primed pcr. | arbitrarily primed pcr (ap-pcr) assays can be used to discriminate between species of leptospira. comparative analysis of the fingerprints obtained from representative sets of serovar reference strains of leptospira interrogans sensu stricto, l. borgpetersenii, and l. kirschneri and the reference strains of the other leptospira spp. revealed species-specific dna fragments. these species-specific sequences were reamplified in order to produce digoxigenin-11-dutp-labeled genomic dna probes that co ... | 1997 | 8968917 |
| effect of leptospira interrogans serovar hardjo infection on progesterone concentrations in heifers. | 1997 | 9004477 | |
| invasion of vero cells and induction of apoptosis in macrophages by pathogenic leptospira interrogans are correlated with virulence. | interactions of virulent leptospira interrogans serovar icterohaemorrhagiae strain verdun with vero cells (african green monkey kidney fibroblasts) and a monocyte-macrophage-like cell line (j774a.1) were assayed by a double-fluorescence immunolabelling method. infectivity profiles were investigated according to (i) the duration of contact between leptospires and eukaryotic cells and (ii) the number of in vitro passages after primary isolation from lethally infected guinea pigs. comparative exper ... | 1997 | 9009336 |
| superoxide dismutase activity and lipid peroxidation in the liver of guinea pig infected with leptospira interrogans. | superoxide dismutase (sod) activity and the degree of lipid peroxidation were studied over a two week period in guinea pigs infected with leptospira interrogans derived from wild mice. the total sod activity in infected host liver increased by four-fold two days after infection; this was followed by a 20% decrease resulting in levels comparable to normal, uninfected liver. during the period of decreasing sod activity after day two, the levels of tba-reactive material (tbars) are increased by thr ... | 1997 | 9018467 |
| identification and characterization of the dtdp-rhamnose biosynthesis and transfer genes of the lipopolysaccharide-related rfb locus in leptospira interrogans serovar copenhageni. | immunity to leptospirosis is principally humorally mediated and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. the only protective antigen identified to date is the leptospiral lipopolysaccharide (lps), which biochemically resembles typical gram-negative lps but has greatly reduced endotoxic activity. little is known about the structure of leptospiral lps. a 2.1-kb ecori fragment from the chromosome of serovar copenhageni was cloned in puc18 in escherichia ... | 1997 | 9023210 |
| leptospira interrogans and leptospira peptidoglycans induce the release of tumor necrosis factor alpha from human monocytes. | elevated plasma concentrations of the cytokine tumor necrosis factor alpha (tnf alpha) have been observed in patients affected by leptospirosis. in this study we found that a preparation of peptidoglycan of leptospira interrogans, serovar copenhageni, was able to induce the release of tnf alpha from peripheral blood mononuclear cells. tnf alpha induction occurred in a dose dependent manner and was not affected by the endotoxin inhibitor polymixin b. this is the first report on induction of tnf a ... | 1996 | 9026448 |
| serological survey for diseases in free-ranging coyotes (canis latrans) in yellowstone national park, wyoming. | from october 1989 to june 1993, we captured and sampled 110 coyotes (canis latrans) for various diseases in yellowstone national park, wyoming (usa). prevalence of antibodies against canine parvovirus (cpv) was 100% for adults (> 24 months old), 100% for yearlings (12 to 24 months old), and 100% for old pups (4 to 12 months old); 0% of the young pups (< 3 months old) had antibodies against cpv. presence of antibodies against canine distemper virus (cdv) was associated with the age of the coyote, ... | 1997 | 9027690 |
| enzyme-linked immunosorbent assay and immunoblot analysis for detection of antibodies to borrelia burgdorferi in dogs. the impact of serum absorption with homologous and heterologous bacteriae. | sera from 665 apparently healthy dogs were examined for antibodies to the lyme disease spirochete borrelia (b.) burgdorferi by using an elisa with a whole cell sonicate of b. burgdorferi sensu stricto reference strain b31 (atcc 35210) as antigen. to discover false positive reactions due to the unsatisfactory specificity of conventional enzyme-linked immunosorbent assays for b. burgdorferi, sera were absorbed in parallel with both b. burgdorferi and a heterologous sorbent consisting of whole cell ... | 1996 | 9054122 |
| clinical and clinicopathologic abnormalities in greyhounds with cutaneous and renal glomerular vasculopathy: 18 cases (1992-1994) | to determine clinical signs and clinicopathologic abnormalities in greyhounds with cutaneous and renal glomerular vasculopathy and to determine whether there were any differences between dogs with and without renal azotemia. | 1997 | 9074681 |
| protein engineering reveals ancient adaptive replacements in isocitrate dehydrogenase. | evolutionary analysis indicates that eubacterial nadp-dependent isocitrate dehydrogenases (ec 1.1.1.42) first evolved from an nad-dependent precursor about 3.5 billion years ago. selection in favor of utilizing nadp was probably a result of niche expansion during growth on acetate, where isocitrate dehydrogenase provides 90% of the nadph necessary for biosynthesis. amino acids responsible for differing coenzyme specificities were identified from x-ray crystallographic structures of escherichia c ... | 1997 | 9096353 |
| purification and characterization of a na+, k+ atpase inhibitor found in an endotoxin of leptospira interrogans. | we showed previously that the glycolipoprotein fraction prepared from leptospira interrogans inhibited the na+,k+ atpase enzyme purified from brain or kidney and in isolated nephron segments (m. younes-ibrahim, p. burth, m. v. castro faria, b. buffin-meyer, s. marsy, c. barlet-bas, l. cheval, and a. doucet, c. r. acad. sci. paris ser. iii 318:619-625, 1995). in the present communication, we have demonstrated that unsaturated fatty acids such as oleic and palmitoleic acids, which are adsorbed to ... | 1997 | 9119504 |
| detection of an antigenic protein of leptospira interrogans which shares epitopes with the equine cornea and lens. | a protein epitope which is involved in an antigenic relationship between equine ocular tissues and leptospira interrogans was detected in homogenates of the bacterium. the antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reducing agents by means of western blotting. the outer surface of the leptospires appeared to be free of this epitope as was proved by dot-blot and electron microscopic studies. | 1997 | 9125359 |
| outcome of leptospirosis in children. | we conducted a retrospective analysis of 43 consecutive children (35 boys and 8 girls), 4-14 years of age and living in an urban area, who were hospitalized at the instituto de infectologia emilio ribas (sao paulo, brazil) from january 1989 to december 1995 with an acute illness subsequently diagnosed as leptospirosis. epidemiologic data indicated contact with contaminated water in most cases (88%). the patient sera reacted most strongly with leptospira interrogans serovars copenhageni (45%) and ... | 1997 | 9129533 |
| leptospirosis in brushtail possums: is leptospira interrogans serovar balcanica environmentally transmitted? | in new zealand, the biological control of introduced brushtail possums (trichosurus vulpecula) may be the only affordable option for achieving a significant long term reduction in pest numbers on a national scale. leptospira interrogans serovar balcanica is among the potential biocontrol agents and vectors currently being investigated for this purpose. as the transmission pathways of l. interrogans serovar balcanica between possums are poorly understood, the objective of the study was to determi ... | 1997 | 9131555 |
| serologic survey for infectious pathogens in free-ranging american bison. | from november 1991 through march 1992, we evaluated 101 free-ranging american bison (bison bison) from yellowstone national park, wyoming (usa) for exposure to infectious organisms that commonly infect cattle. no titers were detected for bluetongue virus, bovine leukemia virus, or campylobacter fetus in these 101 bison. detectable antibodies occurred against anaplasma marginale (eight of 76, 11%), bovine respiratory syncytial virus (31 of 101, 31%), bovine viral diarrhea (31 of 101, 31%), bovine ... | 1997 | 9131564 |
| rapid distinction between leptospira interrogans and leptospira biflexa by pcr amplification of 23s ribosomal dna. | bacterial specific primers were used to amplify 23s rrna genes from a representative strain from each of the 23 serogroups of the pathogenic leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic leptospira biflexa. only regions of extreme variability, which had been identified on the basis of homology-based search of all the 23s rrna sequences available in genbank database, were sequenced from the amplified products. pcr primers that had the potential to distinguish l. int ... | 1997 | 9163900 |
| [the pcr amplification, cloning, sequencing, expression in e. coli of gene encoding endoflagella subunit protein (fla b) from leptospira interrogans serovar lai]. | a pair of oligonucleotide primers were designed by ourselves to amplify the endoflagella gene of l. interrogans serovar lai. a fragment about 840 bp was generated with pcr and inserted into plasmid puc8 after the fragment and puc8 were digested respectively with bam hi and pst i. a recombinant plasmid (designated as plf1) was obtained. sds-page analysis indicated that a 33 kd was expressed in e. coli jm103 harboring plf1 and the expression level of the protein was 11% of total bacterial soluble ... | 1996 | 9208612 |
| [16s rrna gene pcr-sscp analysis of the reference strains from 15 serovars (14 serogroups) of pathogenic leptospires in china]. | the dnas of reference strains from 15 serovars (14 serogroups) of pathogenic leptospires in china were amplified with 16s rrna gene primers, and then single-strand conformation polymorphism (sscp) of the products were analyzed in 12.5% nondenaturing mini polyacrylamide gel (containing 5% glycerol) combining with silver-staining. all products showed two bands on electrophoresis at different parameters of voltage or current and concentration of gel. it proved that serovar lai, serovar canicola, se ... | 1996 | 9208613 |
| leptospirosis in raccoons in quebec: 2 case reports and seroprevalence in a recreational area. | raccoons may represent a source of leptospires for humans and domestic animals. we describe a case of severe interstitial nephritis associated with the serovar bratislava of leptospira interrogans (1st report in wildlife), and the seroprevalence to 4 leptospire serovars in a recreational area in quebec. | 1997 | 9220134 |
| evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin m antibody in diagnosis of human leptospiral infection. | the panbio leptospira immunoglobulin m (igm) enzyme-linked immunosorbent assay (elisa) is a commercially available screening test for the diagnosis of acute leptospiral infection. the ability of the test to diagnose early or recent leptospira interrogans infection was assessed by testing sera with known microagglutination test (mat) titers to serovars pomona, hardjo, copenhageni, and australis. the igm elisa detected all 41 cases of early or recent leptospiral infection (sensitivity, 100%), with ... | 1997 | 9230359 |
| immunodiagnosis of human leptospirosis by dot-elisa for the detection of igm, igg, and iga antibodies. | a dot-elisa was evaluated using antigen obtained from leptospira interrogans cultures of the serovars brasiliensis, canicola, cynopteri, hebdomadis, and icterohaemorrhagiae for the detection of human igm, igg, and iga. single serum samples from 63 patients with the icterohemorrhagic form of leptospirosis in the acute phase, collected 3-14 days (mean = 7 days) after the onset of symptoms were tested. ten patients were examined during convalescence and followed up for a period of 4-12 months. for ... | 1997 | 9230798 |
| leptospira interrogans serovar icterohaemorrhagiae seropositivity and the reproductive performance of sows. | the reproductive performance of 28 sows seropositive to leptospira interrogans serovar icterohaemorrhagiae was compared with that of 87 leptospira sp. seronegative dams belonging to the same herd. sows were sampled during 1988 to 1993. during this period the herd was not submitted to any kind of intervention (antibiotic therapy, immunoprophylaxis or rodent control). relative risks (rr) of return to heat, mummified fetuses, stillbirth, and weak newborn piglets for infected sows were assessed and ... | 1997 | 9234428 |
| na,k-atpase: a molecular target for leptospira interrogans endotoxin. | on the basis of our report that a glycolipoprotein fraction (glp) extracted from leptospira interrogans contains a potent inhibitor of renal na,k-atpase, we proposed that glp-induced inhibition of na,k-atpase might be the primary cellular defect in the physiopathology of leptospirosis. the present study was designed to test this hypothesis by determining whether or not 1). glp inhibits all the isoforms of na,k-atpase which are expressed in the tissues affected by leptospirosis, 2) na,k-atpase fr ... | 1997 | 9239307 |
| first epidemiological data on pathogenic leptospires isolated on the azorean islands. | insectivores (erinaceus europaeus) and rodents (rattus rattus, r. norvegicus and mus musculus) from different islands of the azores archipelago were found to carry three distinct leptospira interrogans s.l. serovars (copenhageni, icterohaemorrhagiae and ballum) which have never been previously investigated there. the house mouse and the black rat were the major leptospira reservoirs showing isolation rates ranging from 0% for both species (in graciosa) to 88% and 33%, respectively (in sãao migue ... | 1997 | 9258550 |
| [leptospira interrogans in a canine population of greater buenos aires: variables associated with seropositivity]. | we determined the seroprevalence of leptospirosis in a suburban canine population for the purpose of analyzing the association between different individual and environmental variables and seropositivity for leptospirosis. the study, which was cross-sectional, was performed in july 1992 in a neighborhood of greater buenos aires with approximately 9,500 inhabitants and a canine population of around 2,000 animals. we studied a random sample of 223 dogs and obtained a blood sample from each. each an ... | 1997 | 9312417 |
| differentiation of leptospira interrogans isolates by is1500 hybridization and pcr assays. | genetic variability among leptospira interrogans (sensu stricto) serovars was assessed by southern blot hybridization and pcr analyses. the experiments used probes directed to sequences in a recently described insertion element, is1500. hybridization analysis showed that is1500 was present on polymorphic fragments and that differences in these patterns could be used to identify serovars. hybridization analysis was also useful in discriminating between serovar pomona type kennewicki isolates, mak ... | 1997 | 9316917 |
| identification of a 35-kilodalton serovar-cross-reactive flagellar protein, flab, from leptospira interrogans by n-terminal sequencing, gene cloning, and sequence analysis. | during the screening of antibodies to pathogenic leptospires, a murine monoclonal antibody (designated m138) was found to react with various serovars. an antigen of approximately 35 kda from leptospira interrogans serovar pomona, which reacted strongly with m138, was characterized by n-terminal amino acid sequencing and identified as a flagellin, a class b polypeptide subunit (flab) of the periplasmic flagella. the gene encoding the flab protein, flab, was amplified from the genomic dna of sever ... | 1997 | 9317049 |
| risk factors associated with the likelihood of leptospiral seropositivity in horses in the state of new york. | to determine and quantify risk factors associated with exposure of horses to the following serovars of leptospira interrogans: pomona, autumnalis, and bratislava. | 1997 | 9328661 |
| development and initial evaluation of an indirect enzyme-linked immunosorbent assay for the detection of leptospira interrogans serovar hardjo antibodies in bovine sera. | outer sheath antigen from leptospira interrogans serovar hardjo type hardjoprajitno and acetic acid extracted antigens from serovar hardjo types hardjoprajitno and hardjobovis were evaluated in an immunoassay for ability to detect hyperimmune rabbit serum to serovar hardjo. the degree of cross-reactivity with hyperimmune rabbit sera to l. interrogans serovars pomona, copenhageni, grippotyphosa, canicola and sejroe, and leptospira biflexa serovar patoc was also measured for each antigen. all of t ... | 1997 | 9342449 |
| development of a monoclonal antibody-based competitive enzyme-linked immunosorbent assay for the detection of leptospira borgpetersenii serovar hardjo type hardjobovis antibodies in bovine sera. | a murine monoclonal antibody (designated m553) that binds to an epitope on whole cell antigens prepared from leptospira borgpetersenii serovar hardjo type hardjobovis and leptospira interrogans serovar hardjo type hardjoprajitno, was produced and incorporated into a competitive enzyme-linked immunosorbent assay for the detection of bovine antibodies to serovar hardjo. the epitope recognized by m553 was susceptible to periodate oxidation. the m553 antibody was characterized by western blot with h ... | 1997 | 9342450 |
| simplified analysis of pathogenic leptospiral serovars by random amplified polymorphic dna fingerprinting. | a rapid, simplified procedure combining random amplified polymorphic dna (rapd) fingerprinting of boiled cultures with high resolution agarose gel electrophoresis was used to compare strains from 46 pathogenic leptospiral serovars. the serovars were placed in eight groups on the basis of rapd profile similarities. groups 1-7 corresponded with the genome species leptospira interrogans, l. borgpetersenii, l. santarosai, l. noguchii, l. weilii, l. kirschneri and l. meyeri. the eighth group did not ... | 1997 | 9368533 |
| effect of leptospira interrogans endotoxin on renal tubular na,k-atpase and h,k-atpase activities. | 1997 | 9405892 | |
| [development of polymerase chain reaction-based test systems for detecting leptospira in polytypical leptospirosis foci]. | two highly sensitive test systems g and b, based on the polymerase chain reaction, were developed for indication of pathogenic leptospira interrogans, including the serovariants appearing during outbreaks in polytypical foci of leptospirosis in the tropical zone of china. these test systems can be used for rapid diagnosis of leptospirosis in humans in foci with different etiological structure. | 1997 | 9411214 |
| preliminary survey of leptospirosis and lyme disease amongst febrile patients attending community hospital ambulatory care in maputo, mozambique. | to evaluate the importance of the spirochetes leptospira interrogans s.l. and borrelia burgdorferi s.l., as causes of human diseases (leptospirosis and lyme borreliosis), in order to guide the development of laboratory services and patient management and to identify the appropriateness of future epidemiological studies. | 1997 | 9431763 |
| evaluation of the indirect hemagglutination assay for diagnosis of acute leptospirosis. | serology plays an important role in the diagnosis of leptospirosis. few laboratories have the resources and expertise to perform the microscopic agglutination test. there is a need for rapid and simple serological tests which facilitate the early diagnosis of leptospirosis, while antibiotic therapy may be most effective. a commercially available indirect hemagglutination assay (iha; mrl diagnostics, cypress, calif.) was evaluated with multiple serum specimens from 107 patients being investigated ... | 1998 | 9431911 |
| direct sulfhydrylation for methionine biosynthesis in leptospira meyeri. | a gene library of the leptospira meyeri serovar semaranga strain veldrat s.173 dna has been constructed in a mobilizable cosmid with inserts of up to 40 kb. it was demonstrated that a leptospira dna fragment carrying mety complemented escherichia coli strains carrying mutations in metb. the latter gene encodes cystathionine gamma-synthase, an enzyme which catalyzes the second step of the methionine biosynthetic pathway. the mety gene is 1,304 bp long and encodes a 443-amino-acid protein with a m ... | 1998 | 9440513 |
| coiling phagocytosis discriminates between different spirochetes and is enhanced by phorbol myristate acetate and granulocyte-macrophage colony-stimulating factor. | the mechanisms involved in coiling phagocytosis are not yet known, and it is not even clear whether this phenomenon is either an incidental event or a specific response. therefore, the phagocytic uptake of borrelia burgdorferi and other spirochetes by human monocytes in vitro was used to investigate the involvement of both sides--microbes and phagocytes--in coiling phagocytosis. as seen with electron microscopy, morphologically similar borrelia, leptospira and treponema strains induced markedly ... | 1998 | 9453619 |
| demonstration of leptospiral antigens on tissues using monoclonal antibodies and avidin-biotin peroxidase staining. | glycolipoprotein (glp) cytotoxin was extracted from leptospira interrogans serovar canicola. the silver staining profile of glp subjected to sds-page under denaturing conditions showed a number of bands in the mol. weight range of 14-66 kda. mouse monoclonal antibodies (mabs) igg3 recognizing a band near to 24 kda of leptospiral glp were produced (clone number mglp-01). the agglutinating property of mabs was established by microscopic agglutination test (mat) using 25 different serovars as antig ... | 1997 | 9495655 |
| chronic hepatitis associated with leptospiral infection in vaccinated beagles. | sixteen juvenile beagle dogs originating from a single breeding colony and regularly vaccinated against leptospira interrogans (serogroups canicola and icterohaemorrhagiae) developed a clinical syndrome characterized by retarded growth, weight loss and often ascites. over a 10-month period, post-mortem examinations were performed on all affected dogs. gross lesions were confined to the liver which was often firm, tan-coloured and mottled. microscopically, hepatic lesions ranged from those of sev ... | 1997 | 9502268 |
| cloned mammalian neutral sphingomyelinase: functions in sphingolipid signaling? | sphingomyelin is an abundant constituent of the plasma membranes of mammalian cells. ceramide, its primary catabolic intermediate, is released by either acid sphingomyelinase or neutral sphingomyelinase (nsmase) and has emerged as a potential lipid signaling molecule. nsmase is regarded as a key enzyme in the regulated activation of the "sphingomyelin cycle" and cell signaling. we report here the cloning, identification, and functional characterization of murine and human nsmase, a ubiquitously ... | 1998 | 9520418 |
| characterization of leptospiral outer membrane lipoprotein lipl36: downregulation associated with late-log-phase growth and mammalian infection. | we report the cloning of the gene encoding a 36-kda leptospiral outer membrane lipoprotein, designated lipl36. we obtained the n-terminal amino acid sequence of a staphylococcal v8 proteolytic-digest fragment in order to design an oligonucleotide probe. a lambda-zap ii library containing ecori fragments of leptospira kirschneri dna was screened, and a 2.3-kb dna fragment which contained the entire structural lipl36 gene was identified. several lines of evidence indicate that lipl36 is lipid modi ... | 1998 | 9529084 |
| unconventional genomic organization in the alpha subgroup of the proteobacteria. | pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family rhizobiaceae of the alpha subgroup of the class proteobacteria. the number and sizes of replicons were determined by separating nondigested dna. hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. members of the genus agrobacterium all possess two chromosomes, and each biovar has a specific genome size. as previously demonstrated ... | 1998 | 9573163 |
| structure and expression of the flaa periplasmic flagellar protein of borrelia burgdorferi. | the spirochete which causes lyme disease, borrelia burgdorferi, has many features common to other spirochete species. outermost is a membrane sheath, and within this sheath are the cell cylinder and periplasmic flagella (pfs). the pfs are subterminally attached to the cell cylinder and overlap in the center of the cell. most descriptions of the b. burgdorferi flagellar filaments indicate that these organelles consist of only one flagellin protein (flab). in contrast, the pfs from other spirochet ... | 1998 | 9573194 |
| detection of leptospira dna in patients with aseptic meningitis by pcr. | samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by pcr for detection of leptospires, and the results were compared with those of the microscopic agglutination test (mat) and an enzyme-linked immunosorbent assay for detection of immunoglobulin m (elisa-igm). of these samples, 39.80% were positive by pcr and 8.74 and 3.88% were positive by mat and elisa-igm, respectively. | 1998 | 9574730 |
| leptospirosis in rehabilitated pacific harbor seals from california. | renal disease was observed in two rehabilitated pacific harbor seals (phoca vitulina richardsii) from a facility in california (usa). the seals had leukocytosis and high serum phosphorus, blood urea nitrogen and creatinine concentrations. a retrospective study of leptospiral antibody serum titers indicated both seals had elevated titers to leptospira interrogans serovar grippotyphosa. a third seal, which died about the time when the index cases occurred, also had elevated titers to l. interrogan ... | 1998 | 9577797 |
| leptospira genomics. | the bacterial species leptospira interrogans (sensu stricto) has a complex genome containing two circular chromosomal replicons. comparative analysis of the larger chromosome reveals a fluid genetic organization with many large rearrangements differentiating two closely related strains. in the present study new genes were identified by partial sequence analysis of randomly cloned fragments of l. interrogans dna. these genes were localized in regions of the genome by nucleic acid hybridization wi ... | 1998 | 9588807 |
| differentiation of pathogenic and non-pathogenic leptospires by means of the polymerase chain reaction. | a polymerase chain reaction was carried out to detect pathogenic leptospires isolated from animals and humans in argentina. a double set of primers (g1/g2, b64-i/b64-ii), described before, were used to amplify by pcr a dna fragment from serogroups belonging to leptospira interrogans but did not allow to detect saprophytic strains isolated from soil and water (l. biflexa). this fact represents an advantage since it makes possible the differentiation of pathogenic from non-pathogenic leptospires i ... | 1997 | 9640782 |
| molecular characterization and postsplicing fate of three introns within the single rrna operon of the hyperthermophilic archaeon aeropyrum pernix k1. | the single rrna operon (arns-arnl) of the hyperthermophilic archaeon aeropyrum pernix k1 was sequenced. the dna sequence data and detailed rna analyses disclosed an unusual feature: the presence of three introns at hitherto undescribed insertion positions within the rrna genes. the 699-nucleotide (nt) intron ialpha was located at position 908 (escherichia coli numbering [h. f. noller, annu. rev. biochem. 53:119-162, 1984]) of the 16s rrna, while the 202-nt intron ibeta and 575-nt intron igamma w ... | 1998 | 9658008 |
| physical and genetic maps of the leptospira interrogans serovar icterohaemorrhagiae strain ictero no.1 chromosome and sequencing of a 19-kb region of the genome containing the 5s rrna gene. | we report the construction of physical and genetic maps of the chromosome of leptospira interrogans serovar icterohaemorrhagiae strain ictero no.1 using pulsed-field gel electrophoresis of dna fragments generated by digestion with enzymes srfi, asci, fsei, and noti and using reciprocal hybridization. we also sequenced the 19-kilobase (kb) dna segment including the one gene for 5s rrna (rrf) of pathogenic leptospira. the size of the chromosome of the strain ictero no.1 was estimated to be 4673kb ... | 1998 | 9666070 |
| morphological characterization of lung and kidney lesions in c3h/hej mice infected with leptospira interrogans serovar icterohaemorrhagiae: defect of cd4+ and cd8+ t-cells are prognosticators of the disease progression. | neonates and young c3h/hej mice were highly susceptible to lethal infection with leptospira interrogans serovar icterohaemorrhagiae. the main pathological changes were seen by light microscopy in the lung and kidneys of 3-week-old mice at 11 days after inoculation. lung histological lesions included small and medium-sized vasculitis with fibrinoid changes, hemorrhages, moderate infiltrate of mononuclear inflammatory cells and fibrin thrombi. in the kidney there was mild to severe acute tubular n ... | 1998 | 9681649 |
| physical and genetic map of the obligate intracellular bacterium coxiella burnetii. | pulsed-field gel electrophoresis and pcr techniques have been used to construct a noti macrorestriction map of the obligate intracellular bacterium coxiella burnetii nine mile. the size of the chromosome has been determined to be 2,103 kb comprising 29 noti restriction fragments. the average resolution is 72.5 kb, or about 3. 5% of the genome. experimental data support the presence of a linear chromosome. published genes were localized on the physical map by southern hybridization. one gene, rec ... | 1998 | 9683477 |
| transmission of leptospira interrogans serovar balcanica infection among socially housed brushtail possums in new zealand. | leptospira interrogans serovar balcanica is a potential vector being investigated for spreading a biological control agent among introduced brushtail possums (trichosurus vulpecula) in new zealand. as previous studies have shown that possums are unlikely to contract leptospirosis through a contaminated environment alone, the objective was to determine whether l. interrogans serovar balcanica could be transmitted between sexually mature, socially housed possums. possums were infected experimental ... | 1998 | 9706567 |
| molecular analysis of the dnak locus of leptospira interrogans serovar copenhageni. | analysis of the dnak locus of leptospira interrogans serovar copenhageni identified four genes in the order hrca, grpe, dnak and dnaj. this is the first time a homologue of hrca has been identified in a spirochete. the hrca gene and a regulatory sequence, designated circe, play a significant role in the regulation of the dnak locus of several gram+ organisms. their presence upstream of dnak in leptospira suggested a similiar regulatory mechanism. transcriptional analysis using reverse transcript ... | 1998 | 9714717 |
| insertion sequences. | insertion sequences (iss) constitute an important component of most bacterial genomes. over 500 individual iss have been described in the literature to date, and many more are being discovered in the ongoing prokaryotic and eukaryotic genome-sequencing projects. the last 10 years have also seen some striking advances in our understanding of the transposition process itself. not least of these has been the development of various in vitro transposition systems for both prokaryotic and eukaryotic e ... | 1998 | 9729608 |
| a natural large chromosomal inversion in lactococcus lactis is mediated by homologous recombination between two insertion sequences. | comparative analysis of chromosomal macrorestriction polymorphism of the two closely related lactococcus lactis subsp. cremoris strains mg1363 and ncdo763 revealed the presence of a large inversion covering half of the genome. to determine what kind of genetic element could be implicated in this rearrangement, the two inversion junctions of mg1363 and ncdo763 chromosomes were cloned and characterized. nucleotide sequence analysis showed the presence of one copy of the lactococcal is905 element i ... | 1998 | 9733685 |
| epidemiologic features of equine leptospira interrogans of human significance. | leptospirosis is a zoonotic bacterial disease caused by leptospira interrogans. there is a serologic evidence that horses are exposed to l. interrogans and, as a shedder of these organisms, can be a threat to humans. we examined risk factors associated with the risk of testing seropositive to three l. interrogans serovars (l. icterohaemorrhagiae, l. grippotyphosa, and l. canicola) in the horses of new york state, in order to understand the epidemiology of the disease and suggest strategies to co ... | 1998 | 9762736 |
| first report of human leptospirosis due to leptospira interrogans serovar javanica in india. | 1998 | 9764329 | |
| macroscopic agglutination test for rapid diagnosis of human leptospirosis. | a commercially available slide agglutination test (sat) for the diagnosis of human leptospirosis was evaluated by comparing it to an immunoglobulin m (igm) enzyme-linked immunosorbent assay (elisa) and to the microscopic agglutination test (mat). for all 108 patients, leptospirosis was diagnosed on the basis of a fourfold or greater increase in titer by mat (seroconversion), and all but 1 of 245 controls were mat negative (titers, <1:100). both sat and the igm elisa failed to detect one case of ... | 1998 | 9774553 |
| identification and recombinant expression of a mycobacterium avium rhamnosyltransferase gene (rtfa) involved in glycopeptidolipid biosynthesis. | the mycobacterium avium complex is a source of disseminated infections in patients with advanced aids. this group of mycobacteria is distinguished by the presence of highly antigenic, surface-exposed glycopeptidolipids, and these glycolipids possess variant oligosaccharide structures that are the chemical basis of the 28 distinct serovars of the m. avium complex. we previously described the ser2 gene cluster, encoding the synthesis of the haptenic oligosaccharide (2, 3-dimethylfucose-rhamnose-6- ... | 1998 | 9791104 |
| prevalence and geographic origin of pigs with serological evidence of infection with leptospira interrogans serovar pomona slaughtered in abattoirs in victoria, australia. | a set of 10,440 sera was collected from pigs slaughtered at victorian abattoirs. these sera were subjected to the microscopic agglutination test for antibodies to leptospira interrogans serovar pomona. identification of the herd of origin was possible for 6511 pigs, and these were derived from 167 herds in victoria (84% of sera), from 32 herds in new south wales (8% of sera) and 29 herds in south australia (8% of sera). the overall prevalence of titres of 512 and above was 3.7%. this was higher ... | 1998 | 9791870 |
| [investigation on the rate of urinary excretion of leptospires among cattle naturally infected with leptospira interrogans]. | urine samples of leptospires from cattle were detected by polymerase chain reaction (pcr) and isolation. positive rates by southern-blot and agarose gel electrophoresis of pcr amplification productions were 13% and 11% respectively. positive rate of isolation was 3.1%. various positive rates by pcr for cattle urine from different areas were discovered. average rate of urinary excretion of leptospires among cattle that naturally infected with leptospira interrogans was 13.2%. data showed that cat ... | 1997 | 9812473 |
| leptospira interrogans antibodies in feral pigs from new south wales. | the sera of 195 hunter-killed feral pigs (sus scrofa), collected in new south wales (australia) from april to november 1995, were screened against a reference panel of 14 leptospira interrogans serovars using a microscopic agglutination test (mat). the panel represented those serovars previously isolated from wild and domestic mammals in mainland australia. antileptospiral agglutinins were detected in 20% of the sera tested and included nine l. interrogans serovars. the majority of serological r ... | 1998 | 9813843 |
| serosurvey of selected viral and bacterial diseases in wild swine from oklahoma. | blood samples collected from 120 wild swine (sus scrofa) in thirteen oklahoma (usa) counties during 1996 were tested for antibodies against six viral and two bacterial diseases. no antibodies to swine brucellosis, pseudorabies, transmissible gastroenteritis, and vesicular stomatitis were detected. antibody titers to one or more leptospiral serovars were found in 44% of the samples, the two most frequent serovars being leptospira interrogans serovars bratislava (29%) and pomona (27%). antibody ag ... | 1998 | 9813859 |
| leptospirosis - an emerging pathogen in travel medicine: a review of its clinical manifestations and management. | leptospirosis is a zoonosis caused by a spirochete, leptospira interrogans. the disease has a wide range of clinical manifestations, ranging from a mild febrile illness to classic weil's disease, which presents with jaundice, myalgia, acute renal failure, and tissue hemorrhage. transmission usually occurs from contact with infected water surfaces. recently, recreational activities such as hiking, swimming in lakes, biking through infected water, and hunting have been associated with the acquisit ... | 1996 | 9815435 |
| [standardization of the lethal dose 50% of leptospira interrogans strains used in the control of the leptospirosis cuban vaccine for human use]. | the ld50 of strains of l. interrogans used in the potency assay of the cuban trivalent leptospiral vaccine intended for human use was standardized. the control of the leptospires content of the cultures by means of the adjusting dilution (10 to 12 leptospires per field) was introduced. that was the key of the standardization of the assay, since it guaranteed the reproducibility of the estimate of the ld50 value for each serogroup: 10(5.98) for canicola, 10(5.60) for icterohaemorrhagiae and 10(6. ... | 1998 | 9842263 |
| in vivo apoptosis of hepatocytes in guinea pigs infected with leptospira interrogans serovar icterohaemorrhagiae. | to investigate the contribution of the previously demonstrated in vitro apoptosis to the pathogenesis of leptospirosis, guinea pigs were infected with leptospira interrogans serovar icterohaemorrhagiae strain verdun and sequentially killed to collect target organs involved in the natural history of the disease (liver, kidneys, lungs, spleen and heart). the combination of histopathological procedures and a specific tunel assay showed a significant leptospira-induced programmed cell death of hepat ... | 1998 | 9851039 |
| apoptosis of lymphocytes in mice administered lipopolysaccharide from leptospira interrogans. | we report that administration of leptospiral lipopolysaccharide (lps) in mice results in massive surface marker changes in the lymphocytes of the spleen. it appears that many of these changes relate to the large number of cells undergoing apoptosis. it is also shown that tumor necrosis factor-alpha (tnf-alpha) induces similar effects and is produced in large quantities after injection of leptospiral lps. it is likely that cytokines such as tnf-alpha are involved in apoptosis. | 1998 | 9852768 |
| [demonstration of intraocular leptospira in 4 horses suffering from equine recurrent uveitis (eru)]. | vitreous samples from 43 horses which underwent vitrectomy because of equine recurrent uveitis (eru) were cultured for leptospires. out of 4 vitreous samples (4/43 = 9%), leptospires could be isolated. in 3 cases, serovar grippotyphosa, and in one case, a serovar out of the serogroup australis were identified. so for the first time, in several horses with eru in vivo cultures of vitreous material were positive for leptospires. a strong evidence of association between leptospiral infection and uv ... | 1998 | 9880935 |
| expression and distribution of leptospiral outer membrane components during renal infection of hamsters. | the outer membrane of pathogenic leptospira species grown in culture media contains lipopolysaccharide (lps), a porin (ompl1), and several lipoproteins, including lipl36 and lipl41. the purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. hamsters were challenged with host-derived leptospira kirschneri to generate sera which contained antibodies to antigens expressed in vivo. immunoblotting performed with sera from ani ... | 1999 | 9916100 |