Publications

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diversity, in-vitro virulence traits and antifungal susceptibility pattern of gastrointestinal yeast flora of healthy poultry, gallus gallus domesticus.poultry farming and consumption of poultry (gallus gallus domesticus) meat and eggs are common gastronomical practices worldwide. till now, a detailed understanding about the gut colonisation of gallus gallus domesticus by yeasts and their virulence properties and drug resistance patterns in available literature remain sparse. this study was undertaken to explore this prevalent issue.201728506251
enhanced expression of lipase i from galactomyces geotrichum by codon optimisation in pichia pastoris.relatively poor heterologous protein yields have limited the commerical applications of galactomyces geotrichum lipase i (ggl i) efficacy trials. to address this, we have redesigned the ggl i gene to preferentially match codon frequencies of pichia pastoris (p. pastoris) while retaining the same amino acid sequence. the wild type and codon optimised ggl i (ggl i-wt and ggl i-op) were synthesised and cloned into ppiczαa with an n-terminal 6 × his tag sequence and expressed in p. pastoris x 33. th ...201728583876
biodiversity in targeted metabolomics analysis of filamentous fungal pathogens by (1)h nmr-based studies.the taxonomical classification among fungi kingdom in the last decades was evolved. in this work the targeted metabolomics study based on (1)h nmr spectroscopy combined with chemometrics tools was reported to be useful for differentiation of three model of fungal strains, which represent various genus of ascomycota (aspergillus pallidofulvus, fusarium oxysporum, geotrichum candidum) were selected in order to perform metabolomics studies. each tested species, revealed specific metabolic profile o ...201728585165
treatment of tannery effluent by passive uptake-parametric studies and kinetic modeling.galactomyces geotrichum was utilized as a potential biosorbent for the treatment of tannery effluent under controlled environmental conditions. tannery effluent treatment was studied through parametric experiments to study the effect of effluent ph (3.0-10.0), initial cod (1100-4400 mg/l), and biosorbent dosage (0.3-3.0 g/l).the zeta potential of the biosorbent was determined and found to influence the optimal ph. increase in effluent cod values resulted in decreased cod removal percentages whic ...201728597388
fungal diversity of "tomme d'orchies" cheese during the ripening process as revealed by a metagenomic study.tomme d'orchies is an artisanal pressed and uncooked cheese produced and marketed in the north of france. this study aimed at showing the fungal microbiota evolution of this cheese using a metagenetic based illumina technology targeting the its2 domain of 5.8s fungal rdnas. to this end, samples were taken from the rind and the core of different cheeses, after 0, 1, 3, 7, 14 and 21days of ripening. the data underpinned the prevalence of yarrowia lipolytica and galactomyces geotrichum for both mic ...201728806689
geotrichum candidumin infective endocarditis. 201728878529
characterization and synergistic effect of antifungal volatile organic compounds emitted by the geotrichum candidum pf005, an endophytic fungus from the eggplant.plant-associated endophytes are recognized as sources of novel bioactive molecules having diverse applications. in this study, an endophytic yeast-like fungal strain was isolated from the fruit of eggplant (solanum melongena) and identified as geotrichum candidum through phenotypic and genotypic characterizations. this endophytic g. candidum isolate pf005 was found to emit fruity scented volatiles. the compositional profiling of volatile organic compounds (vocs) revealed the presence of 3-methyl ...201728894891
the yeastgeotrichum candidumencodes functional lytic polysaccharide monooxygenases.lytic polysaccharide monooxygenases (lpmos) are a class of powerful oxidative enzymes that have revolutionized our understanding of lignocellulose degradation. fungal lpmos of the aa9 family target cellulose and hemicelluloses. aa9 lpmo-coding genes have been identified across a wide range of fungal saprotrophs (ascomycotina, basidiomycotina, etc.), but so far they have not been found in more basal lineages. recent genome analysis of the yeastgeotrichum candidum(saccharomycotina) revealed the pr ...201728919928
differential microbial diversity in drosophila melanogaster: are fruit flies potential vectors of opportunistic pathogens?drosophila melanogaster has become a model system to study interactions between innate immunity and microbial pathogens, yet many aspects regarding its microbial community and interactions with pathogens remain unclear. in this study wild d. melanogaster were collected from tropical fruits in puerto rico to test how the microbiota is distributed and to compare the culturable diversity of fungi and bacteria. additionally, we investigated whether flies are potential vectors of human and plant path ...201729234354
omics-based insights into flavor development and microbial succession within surface-ripened cheese.in this study, a young cheddar curd was used to produce two types of surface-ripened cheese, using two commercial smear-culture mixes of yeasts and bacteria. whole-metagenome shotgun sequencing was used to screen the microbial population within the smear-culture mixes and on the cheese surface, with comparisons of microorganisms at both the species and the strain level. the use of two smear mixes resulted in the development of distinct microbiotas on the surfaces of the two test cheeses. in one ...201829404426
tannery mixed liquors from an ecotoxicological and mycological point of view: risks vs potential biodegradation application.fungi are known to be present in the activated sludge of wastewater treatment plants (wwtp). their study should be at the base of an overall vision of the plant effectiveness and of effluents sanitary impact. moreover, it could be fundamental for the implementation of successful bioaugmentation strategies aimed at the removal of recalcitrant or toxic compounds. this is one of the first studies on the cultivable autochthonous mycoflora present in the mixed liquors of two wwtp treating either vege ...201829426208
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