Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| atg8 is involved in endosomal and phagosomal acidification in the parasitic protist entamoeba histolytica. | autophagy is one of two major bulk protein degradation systems and is conserved throughout eukaryotes. the protozoan entamoeba histolytica, which is a human intestinal parasite, possesses a restricted set of autophagy-related (atg) proteins compared with other eukaryotes and thus represents a suitable model organism for studying the minimal essential components and ancestral functions of autophagy. e. histolytica possesses two conjugation systems: atg8 and atg5/12, although a gene encoding atg12 ... | 2015 | 25923949 |
| detection and molecular characterisation of giardia duodenalis, cryptosporidium spp. and entamoeba spp. among patients with gastrointestinal symptoms in gambo hospital, oromia region, southern ethiopia. | to assess the prevalence and genetic diversity of the enteric protozoa species g. duodenalis, cryptosporidium spp. and entamoeba histolytica in individuals with gastrointestinal symptoms compatible with infections by these pathogens seeking medical attention in a rural area in southern ethiopia. | 2015 | 25939247 |
| amino acid residues leu135 and tyr236 are required for rna binding activity of cfim25 in entamoeba histolytica. | pre-mrna 3' end processing in the nucleus is essential for mrna stability, efficient nuclear transport, and translation in eukaryotic cells. in human, the cleavage/polyadenylation machinery contains the 25 kda subunit of the cleavage factor im (cfim25), which specifically recognizes two ugua elements and regulates the assembly of polyadenylation factors, poly(a) site selection and polyadenylation. in entamoeba histolytica, the protozoan parasite responsible for human amoebiasis, ehcfim25 has bee ... | 2015 | 25941172 |
| characterization of ph-induced transitions of entamoeba histolytica d-phosphoglycerate dehydrogenase. | entamoeba histolytica d-phosphoglycerate dehydrogenase (ehpgdh) exists as a functionally active homodimer at ph 7. our earlier studies have shown that ionic interactions are essentially required for the oligomeric status and activity of the protein. present study focuses on ph associated structural modulations of ehpgdh. far-uv cd spectra showed loss in the secondary structure of the protein as a function of low ph, however, the protein was not completely unfolded even at ph 2. energy minimized ... | 2015 | 25944370 |
| structure of uridine diphosphate n-acetylglucosamine pyrophosphorylase from entamoeba histolytica. | uridine diphosphate n-acetylglucosamine pyrophosphorylase (uap) catalyzes the final step in the synthesis of udp-glcnac, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. small-molecule inhibitors have been developed against uap from trypanosoma brucei that target an allosteric pocket to provide selectivity over the human enzyme. a 1.8 å resolution crystal structure was determined of uap from entamoeba histolytica, an anaerobic parasitic protozoan that c ... | 2015 | 25945709 |
| structures of a histidine triad family protein from entamoeba histolytica bound to sulfate, amp and gmp. | three structures of the histidine triad family protein from entamoeba histolytica, the causative agent of amoebic dysentery, were solved at high resolution within the seattle structural genomics center for infectious disease (ssgcid). the structures have sulfate (pdb entry 3oj7), amp (pdb entry 3omf) or gmp (pdb entry 3oxk) bound in the active site, with sulfate occupying the same space as the α-phosphate of the two nucleotides. the c(α) backbones of the three structures are nearly superimposabl ... | 2015 | 25945711 |
| structure of an adp-ribosylation factor, arf1, from entamoeba histolytica bound to mg(2+)-gdp. | entamoeba histolytica is the etiological agent of amebiasis, a diarrheal disease which causes amoebic liver abscesses and amoebic colitis. approximately 50 million people are infected worldwide with e. histolytica. with only 10% of infected people developing symptomatic amebiasis, there are still an estimated 100,000 deaths each year. because of the emergence of resistant strains of the parasite, it is necessary to find a treatment which would be a proper response to this challenge. adp-ribosyla ... | 2015 | 25945714 |
| iron status of schoolchildren (6-15 years) and associated factors in rural nigeria. | schoolchildren are vulnerable to anaemia because of their higher iron need to meet the demands of puberty and adolescence. | 2015 | 25952679 |
| a comparative study of bacterial and parasitic intestinal infections in india. | infectious diarrhea causes a major health problem in developing countries with significant morbidity and mortality. very often, rehydration therapy alone does not suffice, mandating the use of antimicrobial agents. however, rapidly decreasing antimicrobial susceptibility is complicating the matters. | 2015 | 25954615 |
| the nlrp3 inflammasome is a pathogen sensor for invasive entamoeba histolytica via activation of α5β1 integrin at the macrophage-amebae intercellular junction. | entamoeba histolytica (eh) is an extracellular protozoan parasite of humans that invades the colon to cause life-threatening intestinal and extra-intestinal amebiasis. colonized eh is asymptomatic, however, when trophozoites adhere to host cells there is a considerable inflammatory response that is critical in the pathogenesis of amebiasis. the host and/or parasite factors that trigger the inflammatory response to invading eh are not well understood. we recently identified that eh adherence to m ... | 2015 | 25955828 |
| [coecum amoebiasis can be a rare manifestation of entamoeba histolytica infection]. | a 58-year-old danish male was admitted to hospital with acute abdominal pain. laparoscopy showed a normal appendix. however, a tumour in the ascending colon was found and hemicolectomy was performed. pathological examination showed deep ulcerations with undermined edges and massive inflammation and necrosis. periodic acid schiff (pas)-positive amoebae trophozoites were identified, and the patient was diagnosed with and treated for amoebiasis. the patient had not been abroad for 14 years but he w ... | 2015 | 25967092 |
| amoeboma-lest we forget!!! | amoebiasis is an infection with the parasitic intestinal protozoan entamoeba histolytica (e. histolytica). most infections are usually asymptomatic, but the disease spectrum can range from dysentery to extraintestinal infections, including liver abscesses. the management of a 68-year-old male with amoeboma, a rare manifestation of intestinal amoebiasis, is described along with a review of literature. | 2015 | 25972693 |
| serological investigation to identify risk factors for post-flood infectious diseases: a longitudinal survey among people displaced by typhoon morakot in taiwan. | after typhoon morakot struck taiwan in 2009, thousands of taiwanese citizens were displaced to shelters for several weeks. others were placed in urban communities where they had family members. this study aimed to investigate serological status in both groups and identify risk factors associated with seroconversion of infectious diseases. | 2015 | 25976763 |
| dbdiasnp: an open-source knowledgebase of genetic polymorphisms and resistance genes related to diarrheal pathogens. | diarrhea is a highly common infection among children, responsible for significant morbidity and mortality rate worldwide. after pneumonia, diarrhea remains the second leading cause of neonatal deaths. numerous viral, bacterial, and parasitic enteric pathogens are associated with diarrhea. with increasing antibiotic resistance among enteric pathogens, there is an urgent need for global surveillance of the mutations and resistance genes primarily responsible for resistance to antibiotic treatment. ... | 2015 | 25978092 |
| entamoeba mitosomes play an important role in encystation by association with cholesteryl sulfate synthesis. | hydrogenosomes and mitosomes are mitochondrion-related organelles (mros) that have highly reduced and divergent functions in anaerobic/microaerophilic eukaryotes. entamoeba histolytica, a microaerophilic, parasitic amoebozoan species, which causes intestinal and extraintestinal amoebiasis in humans, possesses mitosomes, the existence and biological functions of which have been a longstanding enigma in the evolution of mitochondria. we previously demonstrated that sulfate activation, which is not ... | 2015 | 25986376 |
| a rare case of multilocus brain abscess due to entamoeba histolytica infection in a child. | 2015 | 25987125 | |
| parasitism in children aged three years and under: relationship between infection and growth in rural coastal kenya. | parasitic infections, which are among the most common infections worldwide, disproportionately affect children; however, little is known about the impact of parasitic disease on growth in very early childhood. our objective was to document the prevalence of parasitic infections and examine their association with growth during the first three years of life among children in coastal kenya. | 2015 | 25996157 |
| phenotypic and transcriptional profiling in entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance. | antimicrobial chemotherapy is critical in the fight against infectious diseases caused by entamoeba histolytica. among the drugs available for the treatment of amebiasis, metronidazole (mtz) is considered the drug of choice. recently, in vitro studies have described mtz resistance and the potential mechanisms involved. costs to fitness and adaptive responses associated with resistance, however, have not been investigated. in this study we generated an hm-1 derived strain resistant to 12 μm mtz ( ... | 2015 | 25999919 |
| intestinal amebae. | among the entamoeba species that infect humans, entamoeba histolytica causes diseases, entamoeba dispar is a harmless commensal, entamoeba moshkovskii seems to be a pathogen, and the pathogenicity of entamoeba bangladeshi remains to be investigated. species-specific detection needed for treatment decisions and for understanding the epidemiology and pathogenicity of these amebae. antigen-based detection methods are needed for e dispar, e moshkovskii, and e bangladeshi; and molecular diagnostic te ... | 2015 | 26004649 |
| palladium(ii) complexes of os donor n-(di(butyl/phenyl)carbamothioyl)benzamide and their antiamoebic activity. | two promising palladium(ii) compounds of general formula, cis-[pd(l-o,s)2] [where hl-o,s = n-(di(butyl/phenyl)carbamothioyl)benzamide] as metal based antiamoebic drug candidates, have been synthesized. both complexes are characterized in the solid state by ft-ir spectroscopy, tga and single crystal x-ray study, as well as in solution by other spectroscopic techniques such as (1)h and (13)c nmr, and uv-visible. all these studies confirm the coordination of ligands through oxygen and sulphur atoms ... | 2015 | 26005916 |
| simultaneous detection of entamoeba histolytica/dispar, giardia duodenalis and cryptosporidia by immunochromatographic assay in stool samples from patients living in the greater cairo region, egypt. | gastrointestinal infection due to intestinal parasites is an enormous health problem in developing countries and its reliable diagnosis is demanding. therefore, this study aimed at evaluating a commercially available immunochromatographic assay (ica) for the detection of cryptosporidia, giardia duodenalis, and entamoeba histolytica/dispar for its usefulness in the greater cairo region, egypt. stool samples of 104 patients who presented between october 2012 and march 2013 with gastrointestinal sy ... | 2015 | 26018117 |
| [evaluation of the new immunocard stat!® cge test for the diagnosis of amebiasis]. | for many years, microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of intestinal parasites although the polymerase chain reaction (pcr) analysis is increasingly utilized due to its high accuracy. recently, pcr has been approved by the world health organization as the current method of choice for the diagnosis of entamoeba histolytica infection. in this study we evaluated a novel immunochromatographic antigen detection rapid test, immunocardstat c ... | 2015 | 26018388 |
| synthesis, characterization and antiamoebic activity of chalcones bearing n-substituted ethanamine tail. | a series of chalcones (4-21) possessing n-substituted ethanamine were synthesized by the aldol condensation reaction of 1-(4-(2-substituted ethoxy)phenyl)ethanones with different aldehydes preceded by the reaction of 2-chloro n-substituted ethanamine hydrochloride and 4-hydroxy acetophenone. the structure of all the synthesized compounds was elucidated by various spectral and x-ray diffraction studies. the compounds were screened against hm1: imss strain of entamoeba histolytica and cytotoxicity ... | 2015 | 26021707 |
| heat shock protein 90 inhibitors repurposed against entamoeba histolytica. | hsp90 is an essential chaperone responsible for trafficking a vast array of client proteins, which are substrates that hsp90 regulates in eukaryotic cells under stress conditions. the atp-binding n-terminal domain of hsp90 (also known as a ghkl type atpase domain) can serve as a specific drug target, because sufficient structural diversity in the atp-binding pocket of hsp90 allows for ortholog selectivity of hsp90 inhibitors. the primary objective of this study is to identify inhibitors specific ... | 2015 | 26029171 |
| potential amoebicidal activity of hydrazone derivatives: synthesis, characterization, electrochemical behavior, theoretical study and evaluation of the biological activity. | four new hydrazones were synthesized by the condensation of the selected hydrazine and the appropriate nitrobenzaldehyde. a complete characterization was done employing 1h- and 13c-nmr, electrochemical techniques and theoretical studies. after the characterization and electrochemical analysis of each compound, amoebicidal activity was tested in vitro against the hm1:imss strain of entamoeba histolytica. the results showed the influence of the nitrobenzene group and the hydrazone linkage on the a ... | 2015 | 26035095 |
| predicting frequency distribution and influence of sociodemographic and behavioral risk factors of schistosoma mansoni infection and analysis of co-infection with intestinal parasites. | geospatial analysis was used to study the epidemiology of schistosoma mansoni, intestinal parasites and co-infections in an area (ilha das flores) in sergipe, brazil. we collected individually georeferenced sociodemographic, behavioral and parasitological data from 500 subjects, analyzed them by conventional statistics, and produced risk maps by kernel estimation. the prevalence rates found were: s. mansoni (24.0%), trichuris trichiura (54.8%), ascaris lumbricoides (49.2%), hookworm (17.6%) and ... | 2015 | 26054512 |
| chew on this: amoebic trogocytosis and host cell killing by entamoeba histolytica. | entamoeba histolytica was named 'histolytica' (from histo-, 'tissue'; lytic-, 'dissolving') for its ability to destroy host tissues. direct killing of host cells by the amoebae is likely to be the driving factor that underlies tissue destruction, but the mechanism was unclear. we recently showed that, after attaching to host cells, amoebae bite off and ingest distinct host cell fragments, and that this contributes to cell killing. we review this process, termed 'amoebic trogocytosis' (trogo-, 'n ... | 2015 | 26070402 |
| cytotoxicity of protein-carbon nanotubes on j774 macrophages is a functionalization grade-dependent effect. | carbon nanotubes (cnts) are used as carriers in medicine due to their ability to be functionalized with chemical substances. however, cytotoxicity analysis is required prior to use for in vivo models. the aim of this study was to evaluate the cytotoxic effect of cnts functionalized with a 46 kda surface protein from entamoeba histolytica (p46-cnts) on j774a macrophages. with this purpose, cnts were synthesized by spray pyrolysis and purified (p-cnts) using sonication for 48 h. a 46 kda protein, ... | 2015 | 26075262 |
| binding and endocytosis of bovine hololactoferrin by the parasite entamoeba histolytica. | entamoeba histolytica is a human parasite that requires iron (fe) for its metabolic function and virulence. bovine lactoferrin (b-lf) and its peptides can be found in the digestive tract after dairy products are ingested. the aim of this study was to compare virulent trophozoites recently isolated from hamster liver abscesses with nonvirulent trophozoites maintained for more than 30 years in cultures in vitro regarding their interaction with iron-charged b-lf (b-holo-lf). we performed growth kin ... | 2015 | 26090404 |
| protection against amoebic liver abscess in hamster by intramuscular immunization with an autographa californica baculovirus driving the expression of the gal-lectin lc3 fragment. | in a previous study, we demonstrated that oral immunization using autographa californica baculovirus driving the expression of the gal-lectin lc3 fragment (acnpv-lc3) of entamoeba histolytica conferred protection against ala development in hamsters. in this study, we determined the ability of acnpv-lc3 to protect against ala by the intramuscular route as well as the liver immune response associated with protection. results showed that 55% of hamsters im immunized with acnpv-lc3 showed sterile pr ... | 2015 | 26090442 |
| insights into endosomal maturation of human holo-transferrin in the enteric parasite entamoeba histolytica: essential roles of rab7a and rab5 in biogenesis of giant early endocytic vacuoles. | the pathogenic amoeba entamoeba histolytica is one of the causative agents of health hazards in tropical countries. it causes amoebic dysentery, colitis and liver abscesses in human. iron is one of the essential nutritional resources for survival and chronic infection caused by the amoeba. the parasite has developed multiple ways to import, sequester and utilize iron from various iron-binding proteins from its host. in spite of its central role in pathogenesis, the mechanism of iron uptake by th ... | 2015 | 26096601 |
| burden of major diarrheagenic protozoan parasitic co-infection among amoebic dysentery cases from north east india: a case report. | intestinal diarrheagenic polyparasitic infections are among the major public health concerns in developing countries. here we examined stool specimens by microscopy, dna dot blot and polymerase chain reaction (pcr) to evaluate the co-infection of four principal protozoans among amoebic dysentery cases from northeast indian population. the multiplex pcr confirmed entamoeba histolytica (8.1%), entamoeba dispar (4.8%) and mixed infection of both the parasites (3.4%) in 68 of 356 stool specimens tha ... | 2015 | 26099490 |
| molecular detection of the carriage rate of four intestinal protozoa with real-time polymerase chain reaction: possible overdiagnosis of entamoeba histolytica in nigeria. | diarrhea remains the second largest killer of children worldwide, and nigeria ranks number two on the list of global deaths attributable to diarrhea. meanwhile, prevalence studies on potentially diarrheagenic protozoa in asymptomatic carriers using molecular detection methods remain scarce in sub-saharan countries. to overcome sensitivity issues related to microscopic detection and identification of cysts in stool concentrates, real-time polymerase chain reaction (pcr) was used to analyze genomi ... | 2015 | 26101274 |
| metronidazole-triazole conjugates: activity against clostridium difficile and parasites. | metronidazole has been used clinically for over 50 years as an antiparasitic and broad-spectrum antibacterial agent effective against anaerobic bacteria. however resistance to metronidazole in parasites and bacteria has been reported, and improved second-generation metronidazole analogues are needed. the copper catalysed huigsen azide-alkyne 1,3-dipolar cycloaddition offers a way to efficiently assemble new libraries of metronidazole analogues. several new metronidazole-triazole conjugates (mtz- ... | 2015 | 26117821 |
| [drainage of amoebic liver abscess by single incision laparoscopic surgery. report of a case]. | single incision laparoscopic surgery has increased recently due to successful results, achieved in several procedures. the aim of the present work is to present the first case in which single incision laparoscopy is used for the drainage of an amoebic liver abscess. | 2015 | 26118779 |
| [intestinal parasitism in terena indigenous people of the province of mato grosso do sul, brazil]. | considering that intestinal protozoans and helminths infect more than half of the world population, with high prevalence in the poorest regions, the objective of this study was to conduct parasitological research among indigenous terena people established in the state of matogrosso do sul. an inquiry was performed to find the incidence of parasitism in these communities. | 2015 | 26120856 |
| detection of entamoeba histolytica by recombinase polymerase amplification. | amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. traditional microscopy approaches are neither sensitive nor specific for entamoeba histolytica. antigen assays are more specific, but many cases are missed unless tested by molecular methods. although polymerase chain reaction (pcr) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource- ... | 2015 | 26123960 |
| magnetic resonance imaging of pathogenic protozoan parasite entamoeba histolytica labeled with superparamagnetic iron oxide nanoparticles. | the aim of this study was to establish a noninvasive tracking of the pathogenic parasite entamoeba histolytica (eh) after superparamagnetic iron oxide (spio) labeling by magnetic resonance imaging (mri) on a single-cell level in vitro and in vivo in a mouse model for amebic liver abscess (ala). | 2015 | 26135016 |
| dimethylated h3k27 is a repressive epigenetic histone mark in the protist entamoeba histolytica and is significantly enriched in genes silenced via the rnai pathway. | rna interference (rnai) is a fundamental biological process that plays a crucial role in regulation of gene expression in many organisms. transcriptional gene silencing (tgs) is one of the important nuclear roles of rnai. our previous data show that entamoeba histolytica has a robust rnai pathway that links to tgs via argonaute 2-2 (ago2-2) associated 27-nucleotide small rnas with 5'-polyphosphate termini. here, we report the first repressive histone mark to be identified in e. histolytica, dime ... | 2015 | 26149683 |
| molecular biology research to benefit patients with entamoeba histolytica infection. | the development of molecular microbiology has made it possible for us to deepen our understanding of the pathogenesis of amebiasis. research using the trophozoite form of entamoeba histolytica has clearly shown us the importance of the interface between the parasite and host cells in vitro. immuno-pathogenesis after excystation was similarly well advanced by the use of a novel murine model of amebic colitis. however, it is still challenging to apply these findings to clinical and epidemiological ... | 2015 | 26173474 |
| metronidazole hydrazone conjugates: design, synthesis, antiamoebic and molecular docking studies. | metronidazole hydrazone conjugates (2-13) were synthesized and screened in vitro for antiamoebic activity against hm1: imss strain of entamoeba histolytica. six compounds were found to be better inhibitors of e. histolytica than the reference drug metronidazole. these compounds showed greater than 50-60% viability against hela cervical cancer cell line after 72 h treatment. also, molecular docking study was undertaken on e. histolytica thioredoxin reductase (ehthrase) protein which showed signif ... | 2015 | 26174554 |
| identification of human intestinal parasites affecting an asymptomatic peri-urban argentinian population using multi-parallel quantitative real-time polymerase chain reaction. | in resource-limited countries, stool microscopy is the diagnostic test of choice for intestinal parasites (soil-transmitted helminths and/or intestinal protozoa). however, sensitivity and specificity is low. improved diagnosis of intestinal parasites is especially important for accurate measurements of prevalence and intensity of infections in endemic areas. | 2015 | 26183074 |
| in vitro studies of chromone-tetrazoles against pathogenic protozoa, bacteria, and fungi. | in vitro studies to fourteen previously synthesized chromone-tetrazoles and four novel fluorine-containing analogs were conducted against pathogenic protozoan (entamoeba histolytica), pathogenic bacteria (pseudomonas aeruginosa, and staphylococcus aureus), and human fungal pathogens (sporothrix schenckii, candida albicans, and candida tropicalis), which have become in a serious health problem, mainly in tropical countries. | 2015 | 26184131 |
| antiamoebic and antigiardial activity of clerodane diterpenes from mexican salvia species used for the treatment of diarrhea. | terpenoids from salvia species have been identified to possess biological properties as antiprotozoal agents. here, we evaluated the antiamoebic and antigiardial activities of 14 known clerodane and modified clerodane-type diterpenes isolated from five mexican salvia species against entamoeba histolytica and giardia lamblia, and analyzed the effects of the functionalities in decalin ring or in the whole clerodane framework to visualize the structural requirements necessary to produce an antiprot ... | 2015 | 26190323 |
| serosurvey of entamoeba histolytica exposure among tepehuanos population in durango, mexico. | the seroepidemiology of entamoeba histolytica infection in tepehuanos population in mexico is largely unknown. this study aimed to study the seroprevalence and correlates of e. histolytica antibodies in tepehuanos in durango, mexico. through a cross-sectional study, we determined the frequency of e. histolytica igg antibodies in 156 tepehuanos people in durango, mexico using an enzyme-linked immunoassay. furthermore, we studied the association of e. histolytica seroprevalence with the socio-demo ... | 2015 | 26199578 |
| occurrence of blastocystis spp. in uberaba, minas gerais, brazil. | intestinal parasites are a problem for public health all over the world. the infection with blastocystis, a protozoan of controversial pathogenicity, is one of the most common among them all. in this study, the occurrence of intestinal parasites, with emphasis on blastocystis, in patients at the universidade federal do triângulo mineiro was investigated in uberaba (mg) through microscopy of direct smears and fecal concentrates using ritchie's method. feces of 1,323 patients were examined from ap ... | 2017 | 26200960 |
| prevalence of intestinal opportunistic parasites infections in the university hospital of bobo-dioulasso, burkina faso. | gastrointestinal parasites infections are widespread in africa and their prevalence infections vary from country to country. this study aimed at assessing the prevalence of opportunistic intestinal parasites infection and other gastrointestinal parasites infection among patients attending the laboratory of parasitology and mycology of the university hospital souro sanou of bobo-dioulasso. | 2015 | 26217488 |
| data set for the proteomics analysis of the endomembrane system from the unicellular entamoeba histolytica. | entamoeba histolytica is the protozoan parasite agent of amebiasis, an infectious disease of the human intestine and liver. this parasite contact and kills human cells by an active process involving pathogenic factors. cellular traffic and secretion activities are poorly characterized in e. histolytica. in this work, we took advantage of a wide proteomic analysis to search for principal components of the endomembrane system in e. histolytica. a total of 5683 peptides matching with 1531 proteins ... | 2014 | 26217682 |
| possible hematological changes associated with acute gastroenteritis among kindergarten children in gaza. | gastroenteritis is considered one of the leading causes of morbidity and mortality in children especially in developing countries. it is a major childhood problem in gaza and one of the most common etiologic agents of iron deficiency anemia (ida). | 2017 | 26229719 |
| a single rnaseiii domain protein from entamoeba histolytica has dsrna cleavage activity and can help mediate rnai gene silencing in a heterologous system. | dicer enzymes process double-stranded rna (dsrna) into small rnas that target gene silencing through the rna interference (rnai) pathway. dicer enzymes are complex, multi-domain rnaseiii proteins, however structural minimalism of this protein has recently emerged in parasitic and fungal systems. the most minimal dicer, saccharomyces castellii dicer1, has a single rnaseiii domain and two double stranded rna binding domains. in the protozoan parasite entamoeba histolytica 27nt small rnas are abund ... | 2015 | 26230096 |
| ehvps32 is a vacuole-associated protein involved in pinocytosis and phagocytosis of entamoeaba histolytica. | here, we investigated the role of ehvps32 protein (a member of the endosomal-sorting complex required for transport) in endocytosis of entamoeba histolytica, a professional phagocyte. confocal microscopy, tem and cell fractionation revealed ehvps32 in cytoplasmic vesicles and also located adjacent to the plasma membrane. between 5 to 30 min of phagocytosis, ehvps32 was detected on some erythrocytes-containing phagosomes of acidic nature, and at 60 min it returned to cytoplasmic vesicles and also ... | 2015 | 26230715 |
| cell-matrix interactions of entamoeba histolytica and e. dispar. a comparative study by electron-, atomic force- and confocal microscopy. | invasion of tissues by entamoeba histolytica is a multistep process that initiates with the adhesion of the parasite to target tissues. the recognition of the non-invasive entamoeba dispar as a distinct, but closely related protozoan species raised the question as to whether the lack of its pathogenic potential could be related to a weaker adhesion due to limited cytoskeleton restructuring capacity. we here compared the adhesion process of both amebas to fibronectin through scanning, transmissio ... | 2015 | 26231438 |
| post-transcriptional regulation of ribosomal protein genes during serum starvation in entamoeba histolytica. | ribosome synthesis involves all three rna polymerases which are co-ordinately regulated to produce equimolar amounts of rrnas and ribosomal proteins (rps). unlike model organisms where transcription of rrna and rp genes slows down during stress, in e. histolytica rdna transcription continues but pre-rrna processing slows down and unprocessed pre-rrna accumulates during serum starvation. to investigate the regulation of rp genes under stress we measured transcription of six selected rp genes from ... | 2015 | 26247142 |
| high throughput sequencing of entamoeba 27nt small rna population reveals role in permanent gene silencing but no effect on regulating gene expression changes during stage conversion, oxidative, or heat shock stress. | the human parasite entamoeba histolytica has an active rna interference (rnai) pathway with an extensive repertoire of 27nt small rnas that silence genes. however the role of this pathway in regulating amebic biology remains unknown. in this study, we address whether silencing via 27nt small rnas may be a mechanism for controlling gene expression changes during conversion between the trophozoite and cyst stages of the parasite. we sequenced small rna libraries generated from trophozoites, early ... | 2015 | 26248204 |
| the effect of ifn-γ and tgf-β in the functional activity of mononuclear cells in the presence of entamoeba histolytica. | entamoeba histolytica (e. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infections. the aim of this study was to analyse the supernatant of cultures of mononuclear (mn) cells with e. histolytica to determine: 1) the levels of the cytokines ifn-γ and tgf-β, and 2) the amoebicidal activity of mn ce ... | 2015 | 26249205 |
| magnetic removal of entamoeba cysts from water using chitosan oligosaccharide-coated iron oxide nanoparticles. | amebiasis, a major health problem in developing countries, is the second most common cause of death due to parasitic infection. amebiasis is usually transmitted by the ingestion of entamoeba histolytica cysts through oral-fecal route. herein, we report on the use of chitosan oligosaccharide-functionalized iron oxide nanoparticles for efficient capture and removal of pathogenic protozoan cysts under the influence of an external magnetic field. these nanoparticles were synthesized through a chemic ... | 2015 | 26261417 |
| multiple amoebic liver abscess as initial manifestation in hiv sero-positive male. | amoebic liver abscess is the most frequent extra-intestinal manifestation of entamoeba histolytica infection. immunosuppression is known to predispose to amoebic liver abscess. although amoebic liver abscess is seen more commonly in patients of human-immunodeficiency virus (hiv), first presentation of hiv sero-positive patient as multiple liver abscess is quite uncommon.the author reports an unusual case of multiple liver abscesses in an hiv seropositive patient. this middle aged male with histo ... | 2015 | 26266151 |
| discovery of ppi-type phosphoenolpyruvate carboxykinase genes in eukaryotes and bacteria. | phosphoenolpyruvate carboxykinase (pepck) is one of the pivotal enzymes that regulates the carbon flow of the central metabolism by fixing co2 to phosphoenolpyruvate (pep) to produce oxaloacetate or vice versa. whereas atp- and gtp-type pepcks have been well studied, and their protein identities are established, inorganic pyrophosphate (ppi)-type pepck (ppi-pepck) is poorly characterized. despite extensive enzymological studies, its protein identity and encoding gene remain unknown. in this stud ... | 2015 | 26269598 |
| taking a bite: amoebic trogocytosis in entamoeba histolytica and beyond. | entamoeba histolytica is a diarrheal pathogen with the ability to cause profound host tissue damage. this organism possesses contact-dependent cell killing activity, which is likely to be a major contributor to tissue damage. e. histolytica trophozoites were recently shown to ingest fragments of living human cells. it was demonstrated that this process, termed amoebic trogocytosis, contributes to cell killing. recent advances in ex vivo and 3-d cell culture approaches have shed light on mechanis ... | 2015 | 26277085 |
| ethanol and isopropanol in concentrations present in hand sanitizers sharply reduce excystation of giardia and entamoeba and eliminate oral infectivity of giardia cysts in gerbils. | enteric protozoan parasites, which are spread by the fecal-oral route, are important causes of diarrhea (giardia duodenalis) and amebic dysentery (entamoeba histolytica). cyst walls of giardia and entamoeba have a single layer composed of fibrils of β-1,3-linked galnac and β-1,4-linked glcnac (chitin), respectively. the goal here was to determine whether hand sanitizers that contain ethanol or isopropanol as the active microbicide might reduce transmission of these parasites. we found that treat ... | 2015 | 26282413 |
| therapy of liver abscesses. | liver abscess (la) is an uncommon but potentially life-threatening disease with significant morbidity and mortality. | 2014 | 26287275 |
| intestinal parasitic infections and anaemia among pregnant women in the highlands of papua new guinea. | this study determined the prevalence of intestinal parasitic infections and associations with risk factors among pregnant women in their second or third trimester in goroka, eastern highlands province, papua new guinea. among the 201 pregnant women enrolled in this study, 163 (81%) were infected with one or more intestinal parasites. infections with protozoan parasites (65%) were more prevalent than infections with nematodes (31%); protozoan infections included entamoeba histolytica (43%), giard ... | 2015 | 26288929 |
| synthesis and characterization with antineoplastic, biochemical, cytotoxic, and antimicrobial studies of schiff base cu(ii) ion complexes. | copper(ii) complexes containing two schiff base ligands derived from 2-hydroxybenzaldehyde with 2-aminophenol and 3-aminophenol have been synthesized and characterized by means of analytical, magnetic, and spectroscopic methods. bacteria, fungus, entamoeba histolytica, and antineoplastic activities of the synthesized complexes have been determined by monitoring the parameters cell growth inhibition, survival time of tumour mice, time-body relation, causing of intraperitoneal cells and macrophage ... | 2015 | 26294901 |
| pulmonary thromboembolism complicating amebic liver abscess: first reported case in the united states-case report and literature review. | even in the absence of amebic colitis, amebic liver abscess (ala) is the most common extraintestinal complication of entamoeba histolytica infection. in the usa, it is most prevalent in middle aged immigrant males from endemic countries such as africa, mexico, and india. one of the complications of ala is inferior vena cava (ivc) thrombosis, which is believed to result from the mechanical compression of the ivc and the consequent thrombogenic nidus elicited from the resultant inflammatory respon ... | 2015 | 26294987 |
| evaluation of factors affecting real-time pcr performance for diagnosis of entamoeba histolytica and entamoeba dispar in clinical stool samples. | although pcr offers the potential for sensitive detection of parasites there are several pitfalls for optimal performance, especially when dna is extracted from a complex sample material such as stool. with the aid of a sensitive inhibitor control in a duplex real-time pcr (qpcr) for identification of entamoeba histolytica and entamoeba dispar we have evaluated factors that influenced the performance of the qpcr and have suggested a rationale to be used in the analysis of clinical samples. pre-p ... | 2015 | 26296348 |
| anaerobic respiration: in vitro efficacy of nitazoxanide against mitochondriate acanthamoeba castellanii of the t4 genotype. | acanthamoeba is an opportunistic protist pathogen that is responsible for serious human and animal infection. being one of the most frequently isolated protists from the environment, it is likely that it readily encounters microaerophilic environments. for respiration under anaerobic or low oxygen conditions in several amitochondriate protists, decarboxylation of pyruvate is catalyzed by pyruvate ferredoxin oxidoreductase instead of pyruvate dehydrogenase. in support, nitazoxanide, an inhibitor ... | 2015 | 26297676 |
| structure-based design of inhibitors of the crucial cysteine biosynthetic pathway enzyme o-acetyl serine sulfhydrylase. | the cysteine biosynthetic pathway is of fundamental importance for the growth, survival, and pathogenicity of the many pathogens. this pathway is present in many species but is absent in mammals. the ability of pathogens to counteract the oxidative defences of a host is critical for the survival of these pathogens during their long latent phases, especially in anaerobic pathogens such as entamoeba histolytica, leishmania donovani, trichomonas vaginalis, and salmonella typhimurium. all of these o ... | 2016 | 26303427 |
| entamoeba invadens: identification of a serca protein and effect of serca inhibitors on encystation. | calcium has an important role on signaling of different cellular processes, including growth and differentiation. signaling by calcium also has an essential function in pathogenesis and differentiation of the protozoan parasites entamoeba histolytica and entamoeba invadens. however, the proteins of these parasites that regulate the cytoplasmic concentration of this ion are poorly studied. in eukaryotic cells, the calcium-atpase of the serca type plays an important role in calcium homeostasis by ... | 2015 | 26318877 |
| pervasiveness of intestinal protozoan and worm incursion in idp's (north waziristan agency, kpk-pakistan) children of 6-16 years. | to evaluate the prevalence of intestinal protozoan and worm infestation in children of families having migrated due to military operation. | 2015 | 26338737 |
| a surface membrane protein of entamoeba histolytica functions as a receptor for human chemokine il-8: its role in the attraction of trophozoites to inflammation sites. | entamoeba histolytica trophozoites respond to the presence of il-8, moving by chemotaxis towards the source of the chemokine. il-8 binds to the trophozoite membrane and triggers a response that activates signaling pathways that in turn regulate actin/myosin cytoskeleton organisation to initiate migration towards the chemokine, suggesting the presence of a receptor for il-8 in the parasite. antibodies directed to the human il-8 receptor (cxcr1) specifically recognised a 29 kda protein in trophozo ... | 2015 | 26343219 |
| a whole-genome rnai screen uncovers a novel role for human potassium channels in cell killing by the parasite entamoeba histolytica. | the parasite entamoeba histolytica kills human cells resulting in ulceration, inflammation and invasion of the colonic epithelium. we used the cytotoxic properties of ameba to select a genome-wide rnai library to reveal novel host factors that control susceptibility to amebic killing. we identified 281 candidate susceptibility genes and bioinformatics analyses revealed that ion transporters were significantly enriched among susceptibility genes. potassium (k(+)) channels were the most common tra ... | 2015 | 26346926 |
| novel hemagglutinating, hemolytic and cytotoxic activities of the intermediate subunit of entamoeba histolytica lectin. | galactose and n-acetyl-d-galactosamine (gal/galnac) inhibitable lectin of entamoeba histolytica, a common protozoan parasite, has roles in pathogenicity and induction of protective immunity in mouse models of amoebiasis. the lectin consists of heavy (hgl), light (lgl), and intermediate (igl) subunits. hgl has lectin activity and lgl does not, but little is known about the activity of igl. in this study, we assessed various regions of igl for hemagglutinating activity using recombinant proteins e ... | 2015 | 26354528 |
| the first case of huge amebic intra-abdominal tumor with asymptomatic amebic colitis. | we report a rare case of huge amebic intra-abdominal tumor with asymptomatic amebic colitis. this appears to represent the first report of amebic intra-abdominal tumor. a 31-year-old woman presented to a local doctor with only a sensation of abdominal fullness. abdominal computed tomography (ct) showed a huge intra-abdominal tumor in the left abdominal cavity, and she was referred to our hospital. colonofiberscopy for detailed examination showed multiple slight, discrete ulcers in the cecum. ame ... | 2015 | 26366345 |
| prevalence of anal human papillomavirus infection and risk factors among hiv-positive patients in tokyo, japan. | oncogenic human papillomavirus (hpv) infection, particularly multiple hpv types, is recognized as a necessary cause of anal cancer. however, a limited number of studies have reported the prevalence of anal hpv infection in asia. we determined the prevalence, genotypes, and risk factors for anal hpv infection in japanese hiv-positive men who have sex with men (msm), heterosexual men, and women. | 2015 | 26368294 |
| infection by intestinal parasites, stunting and anemia in school-aged children from southern angola. | intestinal parasites are responsible for morbidity in children worldwide, especially in low income countries. in the present study we determine the prevalence of intestinal parasites and explore its association with anemia and stunting in school-aged children. | 2015 | 26371758 |
| study of the gastrointestinal parasitic fauna of captive non-human primates (macaca fascicularis). | the aim of this study was to examine helminths and protozoans in cynomolgus macaques (macaca fascicularis) imported from registered breeding facilities in china and their relation to health risks for non-human primate handlers in biomedical research centers and in breeding facilities. fresh fecal samples were collected from a total of 443 m. fascicularis and analyzed by copromicroscopical analysis, immunoenzymatic, or molecular assays. as to helminths, whose eggs were shed in 2.03% of the sample ... | 2016 | 26374536 |
| 1h, 13c and 15n nmr assignments of a calcium-binding protein from entamoeba histolytica. | we report almost complete sequence specific (1)h, (13)c and (15)n nmr assignments of a 150-residue long calmodulin-like calcium-binding protein from entamoeba histolytica (ehcabp6), as a prelude to its structural and functional characterization. | 2016 | 26377206 |
| evidence that the entamoeba histolytica mitochondrial carrier family links mitosomal and cytosolic pathways through exchange of 3'-phosphoadenosine 5'-phosphosulfate and atp. | entamoeba histolytica, a microaerophilic protozoan parasite, possesses mitosomes. mitosomes are mitochondrion-related organelles that have largely lost typical mitochondrial functions, such as those involved in the tricarboxylic acid cycle and oxidative phosphorylation. the biological roles of entamoeba mitosomes have been a long-standing enigma. we previously demonstrated that sulfate activation, which is not generally compartmentalized to mitochondria, is a major function of e. histolytica mit ... | 2015 | 26385892 |
| increasing anaerobic acetate consumption and ethanol yields in saccharomyces cerevisiae with nadph-specific alcohol dehydrogenase. | saccharomyces cerevisiae has recently been engineered to use acetate, a primary inhibitor in lignocellulosic hydrolysates, as a cosubstrate during anaerobic ethanolic fermentation. however, the original metabolic pathway devised to convert acetate to ethanol uses nadh-specific acetylating acetaldehyde dehydrogenase and alcohol dehydrogenase and quickly becomes constrained by limited nadh availability, even when glycerol formation is abolished. we present alcohol dehydrogenase as a novel target f ... | 2015 | 26386051 |
| is procalcitonin increased in cases of invasive amoebiasis? a retrospective, observational study. | procalcitonin (pct) levels are commonly used for diagnostic guidance in routine bacterial infections. by contrast, little data are currently available regarding pct in parasitic diseases, and its role in cases of invasive amoebiasis has not yet been described. for this purpose, 35 adult patients with a proven diagnosis of invasive or digestive amoebiasis were included in a 4-year study period. serum pct was retrospectively assessed. results were analysed with regard to the usual inflammatory bio ... | 2015 | 26388549 |
| the amoebicidal effect of ergosterol peroxide isolated from pleurotus ostreatus. | dysentery is an inflammation of the intestine caused by the protozoan parasite entamoeba histolytica and is a recurrent health problem affecting millions of people worldwide. because of the magnitude of this disease, finding novel strategies for treatment that does not affect human cells is necessary. ergosterol peroxide is a sterol particularly known as a major cytotoxic agent with a wide spectrum of biological activities produced by edible and medicinal mushrooms. the aim of this report is to ... | 2015 | 26392373 |
| pattern of co-infection by enteric pathogenic parasites among hiv sero-positive individuals in a tertiary care hospital, mumbai, india. | one of the major medical concerns in people living with hiv/aids (plha) is management of diarrhea that can lead to severe morbidity and mortality. such clinical scenario warrants an analysis of intestinal parasites, which are important opportunistic pathogens in plha. owing to the scarcity of recent pattern of intestinal opportunistic infections from this region, the study was designed to determine the opportunistic parasites causing diarrhea in plha; and to find out whether there is any signifi ... | 2017 | 26392653 |
| effect of sanitation and water treatment on intestinal protozoa infection: a systematic review and meta-analysis. | pathogenic intestinal protozoa infections are responsible for substantial mortality and morbidity, particularly in settings where people lack improved sanitation and safe drinking water. we assessed the relation between access to, and use of, sanitation facilities and water treatment and infection with intestinal protozoa. | 2016 | 26404667 |
| improved orthologous databases to ease protozoan targets inference. | homology inference helps on identifying similarities, as well as differences among organisms, which provides a better insight on how closely related one might be to another. in addition, comparative genomics pipelines are widely adopted tools designed using different bioinformatics applications and algorithms. in this article, we propose a methodology to build improved orthologous databases with the potential to aid on protozoan target identification, one of the many tasks which benefit from com ... | 2015 | 26416523 |
| entamoeba histolytica dmc1 catalyzes homologous dna pairing and strand exchange that is stimulated by calcium and hop2-mnd1. | meiosis depends on homologous recombination (hr) in most sexually reproducing organisms. efficient meiotic hr requires the activity of the meiosis-specific recombinase, dmc1. previous work shows dmc1 is expressed in entamoeba histolytica, a eukaryotic parasite responsible for amoebiasis throughout the world, suggesting this organism undergoes meiosis. here, we demonstrate dmc1 protein is expressed in e. histolytica. we show that purified ehdmc1 forms presynaptic filaments and catalyzes atp-depen ... | 2015 | 26422142 |
| intestinal amoebiasis in a patient with acute graft-versus-host disease after allogeneic bone marrow transplantation successfully treated by metronidazole. | amoebiasis has rarely been reported in patients undergoing hematopoietic stem cell transplantation, although it is a world-wide infection and extremely common. we present a case of intestinal amoebiasis unexpectedly revealed by colonoscopy after allogeneic bone marrow transplantation from a human leukocyte antigen-mismatched unrelated donor for acute myeloid leukemia arising from chronic myelomonocytic leukemia and successfully treated by metronidazole. | 2015 | 26426525 |
| characterization of the protein tyrosine phosphatase prl from entamoeba histolytica. | protein tyrosine phosphatase of regenerating liver (prl) is a group of phosphatases that has not been broadly studied in protozoan parasites. in humans, prls are involved in metastatic cancer, the promotion of cell migration and invasion. ptps have been increasingly recognized as important effectors of host-pathogen interactions. we characterized the only putative protein tyrosine phosphatase prl (ptp ehprl) in the eukaryotic human intestinal parasite entamoeba histolytica. here, we reported tha ... | 2015 | 26431820 |
| identification of natural inhibitors of entamoeba histolytica cysteine synthase from microbial secondary metabolites. | amebiasis is a common worldwide diarrheal disease, caused by the protozoan parasite, entamoeba histolytica. metronidazole has been a drug of choice against amebiasis for decades despite its known side effects and low efficacy against asymptomatic cyst carriers. e. histolytica is also capable of surviving sub-therapeutic levels of metronidazole in vitro. novel drugs with different mode of action are therefore urgently needed. the sulfur assimilatory de novo l-cysteine biosynthetic pathway is esse ... | 2015 | 26441896 |
| coinfection with malaria and intestinal parasites, and its association with anaemia in children in cameroon. | the purpose of this study was to determine the prevalence of coinfection with malaria and intestinal parasites, as well as to determine its association with anaemia in children aged 10 years and below in muyuka, cameroon. | 2015 | 26445484 |
| prevalence of cryptosporidium parvum/hominis, entamoeba histolytica and giardia lamblia among young children with and without diarrhea in dar es salaam, tanzania. | although enteroparasites are common causes of diarrheal illness, few studies have been performed among children in tanzania. this study aimed to investigate the prevalence of cryptosporidium parvum/hominis, entamoeba histolytica and giardia lamblia among young children in dar es salaam, tanzania, and identify risk factors for infection. | 2015 | 26452235 |
| amoeboid movement in protozoan pathogens. | entamoeba histolytica, the causative agent of amoebiasis, is a protozoan parasite characterised by its amoeboid motility, which is essential to its survival and invasion of the human host. elucidating the molecular mechanisms leading to invasion of human tissues by e. histolytica requires a quantitative understanding of how its cytoskeleton deforms and tailors its mode of migration to the local microenvironment. here we review the wide range of methods available to extract biophysical informatio ... | 2015 | 26459974 |
| molecular epidemiology of entamoeba: first description of entamoeba moshkovskii in a rural area from central colombia. | entamoeba histolytica, e. dispar and e. moshkovskii are the most frequent species described in human infection where e. histolytica is the only true pathogen. the epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the entamoeba species complex. therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. herein, we conducted a cross-sectional study to determine the frequency of ... | 2015 | 26465744 |
| [evaluation of two methods (nativ-lugol preparation and enzyme-linked immunosorbent assay) for detection of entamoeba histolytica in stool samples]. | this study aims to compare the performance of native-lugol examination and eia antigen detection test using stool samples obtained from patients diagnosed as clinical gastroenteritis and submitted to the parasitology laboratory in uludağ university between january 2010 and february 2011. | 2015 | 26470922 |
| [mean platelet volume in children with acute gastroenteritis caused by entamoeba histolytica]. | mean platelet volume (mpv) is a marker of platelet activation, which is a determinant of inflammation. the first aim of the present study was to investigate the mpv levels in children with amebiasis and compare the mpv levels with healthy controls. the second aim of this study was to evaluate the relationship between mpv and other acute phase reactants. | 2015 | 26470926 |
| new drug target in protozoan parasites: the role of thioredoxin reductase. | amebiasis causes approximately 70,000 deaths annually and is the third cause of death due to parasites worldwide. it is treated primarily with metronidazole, which has adverse side effects, is mutagenic and carcinogenic, and emergence of resistance is an increasing concern. unfortunately, better therapeutic alternatives are lacking. re-purposing of older fda approved drugs is advantageous to drug discovery since safety and pharmacokinetic effects in humans are already known. in high throughput s ... | 2015 | 26483758 |
| intestinal parasites detected during pre-employment stool examination at tertiary health care center in makkah, kingdom of saudi arabia. | this retrospective study determined the intestinal parasites discovered in 2490 new employees in a tertiary health care center in makah as regards nationalities, age groups and sex over the period from january 2010 to january 2014 to decrease introduction of communicable diseases. all the data were provided through the electronic hospital information system. the overall positive cases were 16%, significantly higher in females 57.4% than males 42.6%. differences in distribution of positive record ... | 2015 | 26485855 |
| intestinal parasites in patients with chronic abdominal pain. | information about intestinal parasites in sohag (upper egypt) in patients with chronic abdominal pain is scarce. this study determined the intestinal parasites symptoms in 130 patients with chronic abdominal pain and cross-matched 20 healthy persons. parasitic infection was confirmed by stool analysis.the most commonest clinical data with stool analysis was as following: 1-entamoeba histolytica associated with nausea 20 (3 7.74%) followed by anorexia 19 (35.85%), 2-entamoeba coli associated with ... | 2015 | 26485858 |
| intestinal parasites in diabetic patients in sohag university hospitals, egypt. | intestinal parasites usually create benign diseases, though they may induce complications with high morbidity and mortality to the immunocompromised, including diabetic patients. the study detected the prevalence of intestinal parasitic infections in diabetic patients, comparing to non-diabetic controls and other parameters. a total of 100 fecal samples were collected from diabetic patients at the outpatient clinic of sohag university hospitals and another 100 from cross matched controls. the sa ... | 2015 | 26485865 |
| prevalence of intestinal protozoa among saudi patients with chronic renal failure: a case-control study. | it has been hypothesized that chronic renal failure (crf) predisposes patients to infection with intestinal protozoa. we tested this hypothesis with a matched case-control study to determine the prevalence of these protozoa and their diarrhea associated symptoms among 50 patients with crf (cases) from taif, western saudi arabia. fifty diarrheal patients without crf were recruited in the study as controls. participants were interviewed by a structured questionnaire and stool samples were collecte ... | 2015 | 26491455 |
| calpain-like: a ca(2+) dependent cystein protease in entamoeba histolytica cell death. | entamoeba histolytica programmed cell death (pcd) induced by g418 is characterized by the release of important amounts of intracellular calcium from reservoirs. nevertheless, no typical caspases have been detected in the parasite, the pcd phenotype is inhibited by the cysteine protease inhibitor e-64. these results strongly suggest that ca(2+)-dependent proteases could be involved in pcd. in this study, we evaluate the expression and activity of a specific dependent ca(2+) protease, the calpain- ... | 2015 | 26496790 |