Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| lyme disease (borreliosis) in europe. iv lyme disease action conference, sheffield university, united kingdom, 17-18 june 2005. | 2005 | 16496460 | |
| lyme disease: the quest for magic bullets. | lyme disease represents a growing public health threat. recent molecular and genetic studies have confirmed that borrelia burgdorferi, the spirochetal agent of lyme disease, is one of the most complex bacteria known to man. affinity for multiple cell types and the presence of non-replicating forms of b. burgdorferi have contributed to persistent infection and failure of simple antibiotic regimens. the controversial clinical science of lyme disease has impeded reliable diagnosis and effective tre ... | 2006 | 16498239 |
| an in vitro study of the activity of telithromycin against mobile and cystic forms of borrelia afzelii. | in this study the new ketolide telithromycin was tested in vitro against motile and cystic forms of borrelia afzelii, one of the species of borrelia burgdorferi sensu lato. acridine orange staining, dark field microscopy, and transmission electron microscopy were the techniques used to study the influence of telithromycin on the bacteria. the activity was unexpectedly high, 0.0003 microg/ml < mbc < or = 0.0006 microg/ml for the mobile forms after 7 days of incubation at 34 degrees c. mic was < 0 ... | 2006 | 16501899 |
| differentiation of borrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis. | borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing lyme borreliosis. due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated borrelia strains. in the present study, we have optimized tgge for b. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely pfge and restriction of 5s-23s intergenic space region pcr product. a differentiation of b. gari ... | 2006 | 16502459 |
| kinetics of borrelia burgdorferi infection in larvae of refractory and competent tick vectors. | the acquisition of borrelia burgdorferi by the larvae of competent and refractory ixodid ticks was assessed by quantitative polymerase chain reaction (pcr). larvae were fed on infected mice, and the spirochete loads were determined during feeding and up to 93 d postfeeding. amblyomma americanum (l.) was refractory to b. burgdorferi infection, with almost no detection of spirochete dna during or postfeeding. in contrast, ixodes scapularis say supported high loads of spirochetes (10(3)-10(4) per l ... | 2006 | 16506448 |
| detection of a borrelia miyamotoi sensu lato relapsing-fever group spirochete from ixodes pacificus in california. | we investigated whether host-seeking nymphs and adults of the western blacklegged tick, ixodes pacificus cooley & kohls, the primary vector of lyme disease spirochetes in far-western north america, are infected naturally with relapsing-fever group spirochetes in mendocino county, california. relapsing-fever group borreliae were detected in four (1.7%) of 234 nymphal and two (0.7%) of 282 adult host-seeking i. pacificus ticks by polymerase chain reaction and sequence analysis of the 16s rrna and ... | 2006 | 16506458 |
| infectious agents in mucosa-associated lymphoid tissue-type lymphomas: pathogenic role and therapeutic perspectives. | mucosa-associated lymphoid tissue (malt) lymphoma probably constitutes the best in vivo model showing how complex interplay between b lymphocytes and the surrounding microenvironment may lead to a neoplastic disorder. after the seminal discovery of the pathogenic association between helicobacter pylori and gastric malt lymphomas, evidence suggests the possible involvement of other infectious agents in the development of malt lymphomas arising at different body sites. although several other bacte ... | 2006 | 16507206 |
| marginal zone lymphoma. | marginal zone lymphomas (mzls) comprise 3 distinct entities: extranodal mzl of mucosa-associated lymphoid tissue (malt), splenic mzl, and nodal mzl. gastric malt lymphoma is the most common extranodal mzl and often develops as a result of chronic helicobacter pylori gastritis. such cases frequently respond to antibiotics directed against h. pylori. antigen-driven lymphomatous disease can also be seen in the association of borrelia burgdorferi with malt lymphoma of the skin, chlamydia psittaci wi ... | 2006 | 16507274 |
| [erythema migrans due to borrelia afzelii]. | 2006 | 16510103 | |
| borrelia burgdorferi sensu stricto invasiveness is correlated with ospc-plasminogen affinity. | borrelia burgdorferi sensu lato, the causative agent of lyme borreliosis, is transmitted through tick bite. lyme borreliosis evolves in two stages: a primary red skin lesion called erythema migrans; later on, invasive bacteria disseminate to distant sites inducing secondary manifestations (neuropathies, arthritis, carditis, late skin disorders). it has been previously suggested that the ospc gene could be associated with invasiveness in humans depending on its sequence. here, we confirm the patt ... | 2006 | 16513394 |
| clinical and demographic characteristics of psychiatric patients seropositive for borrelia burgdorferi. | borrelia burgdorferi (bb) infection can affect the central nervous system and possibly lead to psychiatric disorders. we compared clinical and demographic variables in bb seropositive and seronegative psychiatric patients and healthy controls. | 2006 | 16516108 |
| [prevalence of antibodies against anaplasma phagocytophilum, babesia microti i borrelia burgdorferi in adults in north-eastern poland]. | of the study was to evaluate the seroprevalance of antibodies against anaplasma phagocytophilum and babesia microti in healthy north-eastern poland, adult population. | 2005 | 16521416 |
| strategies for reducing the risk of lyme borreliosis in north america. | the incidence of lyme borreliosis continues to increase in the united states. in 1991, when lyme borreliosis first became a nationally reportable disease to the centers for disease control and prevention (cdc), a total of 9470 cases were reported; in contrast, by 2002 a total of 23,763 cases were reported, >2.5x the total in 1991. area-wide acaricides can be highly effective in killing nymphal ixodes scapularis, with >95% of nymphs killed in studies using cyfluthrin, deltamethrin, or carbaryl. t ... | 2006 | 16524769 |
| determining the tick scutal index allows assessment of tick feeding duration and estimation of infection risk with borrelia burgdorferi sensu lato in a person bitten by an ixodes ricinus nymph. | the risk of borrelia burgdorferi sensu lato transmission from an infected vector tick to a host increases with increasing duration of tick feeding. in ixodes ricinus nymphs, the main vector of b. burgdorferi s.l. in most parts of europe, the transmission risk appears low to moderate within the first 24h of feeding but increases to >70% after only 36 h. in this study, the so-called scutal index, the ratio between tick abdominal length and scutum width, a very good measure of the level of tick eng ... | 2006 | 16524770 |
| survey on the subject of equine lyme borreliosis. | according to the results of a questionnaire on equine lyme borreliosis addressing veterinarians in germany, the existence of the disease was confirmed by more than half of the 118 participants. practitioners who regarded borrelia burgdorferi as a pathogen of horses seemed to be more sensitized in terms of the number of annually diagnosed cases as well as the frequency of occurrence of tick infestation with equine patients or prophylactic treatments against ectoparasites by horse owners. chronica ... | 2006 | 16524771 |
| clinical aspects of neuroborreliosis and post-lyme disease syndrome in adult patients. | the diagnostic criteria of active neuroborreliosis include inflammatory changes of the cerebrospinal fluid (csf) and an elevated specific borrelia csf-to-serum antibody index, indicating intrathecal borrelia antibody production. patients with neuroborreliosis are usually treated with intravenous ceftriaxone for 2-3 weeks. in case of allergy, doxycycline may be used. treatment efficacy is detected by the improvement of the neurological symptoms and the normalization of the csf pleocytosis. the me ... | 2006 | 16524775 |
| serological evidence for tick-borne encephalitis, borreliosis, and human granulocytic anaplasmosis in mongolia. | five hundred and forty-five serum samples from donors from various parts of mongolia were investigated for antibodies against the tick-borne encephalitis (tbe) virus, borrelia burgdorferi, and anaplasma phagocytophilum. seroprevalence against tbe was 5.1% in the province of selenge and 0.9% in bulgan province, seroprevalence against b. burgdorferi was 1.9% in selenge province and bulgan province, 13.9% in dornogov province, and 3.0% in tov province and ulaanbaatar. seroprevalence against a. phag ... | 2006 | 16524782 |
| lyme disease associated with alzheimer's disease. | this case report discusses a patient with co-occurring neuroborreliosis and alzheimer's disease (ad). although no claim is made for causality nor is there objective evidence that spirochetes are involved in ad, co-infection may exacerbate the symptoms of either neuroborreliosis or ad. much is to be learned about the role of spirochetes in degenerative central nervous system disease. | 2006 | 16528463 |
| role of birds in thuringia, germany, in the natural cycle of borrelia burgdorferi sensu lato, the lyme disease spirochaete. | three hundred and twenty-two birds belonging to 35 species were live-captured in reifenstein, middle germany, from march to october 2004, to investigate their role as hosts of borrelia burgdorferi sensu lato and its vector ticks. a total of 141 ixodes ricinus ticks collected from 53 birds belonging to eight species was investigated for the prevalence of b. burgdorferi by a pcr-rflp analysis of the ospa gene. three of these bird species, the blackbird (turdus merula), the song thrush (t. philomel ... | 2006 | 16530003 |
| comparison of isolation rate of borrelia burgdorferi sensu lato in mkp and bsk-ii medium. | different media have been utilized for borrelial cultivation. the aim of the present study was to evaluate the isolation rate of borrelia burgdorferi sensu lato from two commonly used media, i.e. modified kelly-pettenkofer (mkp) and barbour-stoenner-kelly ii (bsk-ii) medium, and to compare the isolated strains with regard to their phenotypic and genotypic characteristics. skin biopsy specimens of 2 x 2 x 4 mm were taken from the peripheral site of human solitary erythema lesions and were divided ... | 2006 | 16530005 |
| risk of culture-confirmed borrelial persistence in patients treated for erythema migrans and possible mechanisms of resistance. | erythema migrans (em) develops at the site of the tick bite in 77-90% of lyme borreliosis (lb) patients and is therefore a common manifestation of early disease. clinical treatment failures have been reported in early lb cases for almost every suitable antimicrobial agent. the exact risk of resistance to antibiotic treatment in patients with em, however, is not known and there are few published cases of culture-proven treatment failure. moreover, currently available diagnostic techniques cannot ... | 2006 | 16530006 |
| borrelia burgdorferi erp genes are expressed at different levels within tissues of chronically infected mammalian hosts. | the spirochete borrelia burgdorferi is the causative agent of lyme disease and is transmitted to humans and other vertebrate hosts through the bites of ixodid ticks. b. burgdorferi erp (ospe-f related lipoprotein) family members are encoded on members of the 32 kb circular plasmid-like prophage family (cp32s). many erp proteins serve as receptors for the complement inhibitory factor h molecules of numerous vertebrate hosts, providing one mechanism by which the bacteria potentially evade the inna ... | 2006 | 16530008 |
| structure-function mapping of bbcrasp-1, the key complement factor h and fhl-1 binding protein of borrelia burgdorferi. | borrelia burgdorferi, a spirochaete transmitted to human hosts during feeding of infected ixodes ticks, is the causative agent of lyme disease, the most frequent vector-borne disease in eurasia and north america. sporadically lyme disease develops into a chronic, multisystemic disorder. serum-resistant b. burgdorferi strains bind complement factor h (fh) and fh-like protein 1 (fhl-1) on the spirochaete surface. this binding is dependent on the expression of proteins termed complement-regulator a ... | 2006 | 16530476 |
| functionality of borrelia burgdorferi luxs: the lyme disease spirochete produces and responds to the pheromone autoinducer-2 and lacks a complete activated-methyl cycle. | borrelia burgdorferi produces pfs and luxs enzymes for breakdown of the toxic byproducts of methylation reactions, producing 4,5-dihydroxy-2,3-pentanedione (dpd), adenine, and homocysteine. dpd and its spontaneously rearranged derivatives constitute a class of bacterial pheromones named autoinducer-2 (ai-2). we describe that b. burgdorferi produces dpd during laboratory cultivation. furthermore, addition of in vitro synthesized dpd to cultured b. burgdorferi resulted in altered expression levels ... | 2006 | 16530477 |
| prevalence of borrelia burgdorferi s.l. ospa types in ixodes ricinus ticks from selected localities in slovakia and poland. | in this study, 746 questing ixodes (i.) ricinus ticks from eastern slovakia and 187 ticks from southern poland were investigated for infection with borrelia (b.) burgdorferi sensu lato and different outer surface protein a (ospa) types by an improved restriction fragment length polymorphism (rflp) analysis of the ospa gene. the method enables differentiation of both single and multiple infections with b. burgdorferi s.s. (ospa type 1), b. afzelii (ospa type 2), b. garinii (ospa types 3-8), b. va ... | 2006 | 16530478 |
| etiology of tick-borne febrile illnesses in adult residents of north-eastern poland: report from a prospective clinical study. | febrile tick-borne diseases can be caused by different pathogens. the study objective was to prospectively determine the etiology of infection among adults exposed to tick bite and to detect acute human granulocytic anaplasmosis (hga), a recently emerging infection, in north-eastern poland. we evaluated 68 patients (34 males and 34 females, mean age 44.2+/-15 years) who had fever within 4 weeks after a tick bite. paired sera were tested for antibodies against tick-borne encephalitis virus, borre ... | 2006 | 16530481 |
| molecular analysis of decorin-binding protein a (dbpa) reveals five major groups among european borrelia burgdorferi sensu lato strains with impact for the development of serological assays and indicates lateral gene transfer of the dbpa gene. | the borrelia (b.) burgdorferi adhesin dbpa (decorin-binding protein a) is a valuable antigen for serodiagnosis of lyme borreliosis and a promising candidate for a vaccine. to investigate the heterogeneity of dbpa, we aligned dna sequences of 83 different dbpa genes (37 from the database, where the majority of sequences belong to b. burgdorferi sensu stricto and 46 were newly sequenced). analysis of 25 sequences from the species b. burgdorferi s.s., 16 from b. afzelii, 40 from b. garinii, and two ... | 2006 | 16530482 |
| discrimination of cognate and noncognate substrates at the active site of class i lysyl-trna synthetase. | the aminoacyl-trna synthetases are divided into two unrelated structural classes, with lysyl-trna synthetase (lysrs) being the only enzyme represented in both classes. on the basis of the structure of l-lysine complexed with pyrococcus horikoshii class i lysrs (lysrs1) and homology to glutamyl-trna synthetase (glurs), residues implicated in amino acid recognition and noncognate substrate discrimination were systematically replaced in borrelia burgdorferi lysrs1. the catalytic efficiency of stead ... | 2006 | 16533047 |
| immunophenotypic patterns of t-cell activation in neuroinflammatory diseases. | we aimed to gain insights into the pathogen-specific differences in early adaptive immune responses following central nervous system infections with borrelia burgdorferi and viral pathogens by studying the immunophenotypic patterns of t-cell activation. moreover, we wished to determine whether the expression of t-cell activation markers reflects disease activity in multiple sclerosis (ms). | 2006 | 16542164 |
| [investigation of vectors for borrelia burgdorferi and lyme seropositivity in sivas region]. | the aims of this study were to investigate the presence of vector ticks for borrelia burgdorferi and to determine the seropositivity of lyme disease in sivas region of middle anatolia. a total of 10,303 ticks were collected and classified at genus level, however, none of them belonged to ixodes genus, the vector of b. burgdorferi. for the seroepidemiological study, serum samples were collected from 270 subjects who inhabited in rural area and raised livestock, and from 135 subjects as the contro ... | 2005 | 16544552 |
| extension of ixodes ricinus ticks and agents of tick-borne diseases to mountain areas in the czech republic. | along with the shift of the hard tick ixodes ricinus to higher altitudes observed in the czech republic a corresponding shift of tick-borne infections to higher altitudes has been expected. therefore, i. ricinus ticks, mainly nymphs, were investigated for the presence of tick-borne viruses, tick-borne encephalitis (tbe), tribec and eyach, and the spirochaete borrelia burgdorferi sensu lato in the sumava and krkonose mountains (czech republic). the tbe virus and different genospecies of b. burgdo ... | 2006 | 16545603 |
| molecular analyses of the interaction of borrelia hermsii fhba with the complement regulatory proteins factor h and factor h-like protein 1. | borrelia hermsii, the primary etiological agent of tick-borne relapsing fever in north america, binds the complement regulatory protein factor h (fh) as a means of evading opsonophagocytosis and the alternative complement pathway. the ability of fh-binding protein a (fhba) to bind fh-like protein 1 (fhl-1) has not been assessed previously. in this study, using a whole-cell absorption assay, we demonstrated that b. hermsii absorbs both fh and fhl-1 from human serum. consistent with this, affinity ... | 2006 | 16552029 |
| myd88 deficiency enhances acquisition and transmission of borrelia burgdorferi by ixodes scapularis ticks. | borrelia burgdorferi strains exhibit various degrees of infectivity and pathogenicity in mammals, which may be due to their relative ability to evade initial host immunity. innate immune cells recognize b. burgdorferi by toll-like receptors (tlrs) that use the intracellular molecule myd88 to mediate effector functions. to determine whether impaired tlr signaling enhances ixodes scapularis acquisition of b. burgdorferi, we fed nymphs on wild-type (wt) and myd88-/- mice previously infected with tw ... | 2006 | 16552045 |
| human neutrophil calprotectin reduces the susceptibility of borrelia burgdorferi to penicillin. | borrelia burgdorferi, the spirochetal agent of lyme disease, is susceptible to killing by a variety of polymorphonuclear leukocyte (pmn) components. some are most effective against metabolically active b. burgdorferi. the abundant pmn cytoplasmic protein calprotectin, elevated 10- to 100-fold in inflammation, inhibits the growth of spirochetes through chelation of the essential cation, zn. since the action of some therapeutic antibiotics depends on bacterial division, we investigated the antibio ... | 2006 | 16552081 |
| sigma factor selectivity in borrelia burgdorferi: rpos recognition of the ospe/ospf/elp promoters is dependent on the sequence of the -10 region. | members of the ospe/ospf/elp lipoprotein gene families of borrelia burgdorferi, the lyme disease agent, are transcriptionally upregulated in response to the influx of blood into the midgut of an infected tick. we recently have demonstrated that despite the high degree of similarity between the promoters of the ospf (p(ospf)) and ospe (p(ospe)) genes of b. burgdorferi strain 297, the differential expression of ospf is rpos-dependent, while ospe is controlled by sigma(70). herein we used wild-type ... | 2006 | 16553889 |
| a tick b-cell inhibitory protein from salivary glands of the hard tick, hyalomma asiaticum asiaticum. | some studies done to date suggest that b-cell inhibitory factor occurred in tick saliva. in this study, a novel protein having b-cell inhibitory activity was purified and characterized from the salivary glands of the hard tick, hyalomma asiaticum asiaticum. this protein was named b-cell inhibitory factor (bif). the cdna encoding bif was cloned by cdna library screening. the predicted protein from the cdna sequence is composed of 138 amino acids including the mature bif. no similarity was found b ... | 2006 | 16554026 |
| susceptibility of various species of rodents to the relapsing fever spirochete, borrelia hermsii. | in a study to determine susceptibility to borrelia hermsii of various rodents commonly found in or near places where human cases of relapsing fever occurred, chipmunks (eutamias amoenus), pine squirrels (tamiasciurus hudsonicus richardsoni), flying squirrels (glaucomys sabrinus), columbian ground squirrels (spermophilus columbianus columbianus), golden-mantled ground squirrels (s. lateralis tescorum), wood rats (neotoma cinerea cinerea), white-footed deer mice (peromyscus maniculatus), and meado ... | 1970 | 16557828 |
| the incidence and significance of the presence of borrelia vincenti and other spirochaetaceae on beverage glasses. | 1936 | 16559908 | |
| a study of borrelia anserina infection (spirochetosis) in turkeys. | 1949 | 16561664 | |
| the dynamic proteome of lyme disease borrelia. | the proteome of the spirochete bacterium borrelia burgdorferi, the tick-borne agent of lyme disease, has been characterized by two different approaches using mass spectrometry, providing a launching point for future studies on the dramatic changes in protein expression that occur during transmission of the bacterium between ticks and mammals. | 2006 | 16563176 |
| primary biliary cirrhosis: when and why does the disease develop? | primary biliary cirrhosis is a chronic cholestatic liver disease with an autoimmune pathogenesis, that generally develops in adult life, often in perimenopausal age. the clinical features are heterogeneous, ranging from an asymptomatic presentation to end-stage liver disease. primary biliary cirrhosis is unknown in children and its natural history has yet to be elucidated. following a canadian report of primary biliary cirrhosis in two girls (16 and 15 years old), we describe a clinical case dev ... | 2006 | 16567309 |
| differential diagnosis of mesiotemporal lesions: case report of neurosyphilis. | 2006 | 16568298 | |
| ehrlichia infection of the central nervous system. | ehrlichiosis in the united states is caused by three closely related bacterial species (ehrlichia chaffeensis, ehrlichia ewingii, and anaplasma phagocytophilum), all transmitted through tick bite. although there is variation with respect to geography and tick vector, the clinical manifestations are similar, and treatment of these infections is identical. ehrlichiosis can present with a spectrum of neurologic manifestations, ranging in severity from headache to meningoencephalitis. treatment is s ... | 2006 | 16569376 |
| human thyroid autoantigens and proteins of yersinia and borrelia share amino acid sequence homology that includes binding motifs to hla-dr molecules and t-cell receptor. | we previously reported that the spirochete borrelia burgdorferi could trigger autoimmune thyroid diseases (aitd). subsequently, we showed local amino acid sequence homology between all human thyroid autoantigens (human thyrotropin receptor [htsh-r], human thyroglobulin [htg], human thyroperoxidase [htpo], human sodium iodide symporter [hnis]) and borrelia proteins (n = 6,606), and between htsh-r and yersinia enterocolitica (n = 1,153). we have now updated our search of homology with borrelia (n ... | 2006 | 16571084 |
| detection of babesia and anaplasma species in rabbits from texas and georgia, usa. | rabbits have been shown to harbor a suite of zoonotic organisms, including a babesia species, borrelia burgdorferi, and anaplasma phagocytophilum. in this study, we conducted a molecular survey for various tick-borne pathogens in three species of rabbits from texas and georgia. of 18 black-tailed jackrabbits (lepus californicus) tested from texas, six (28%) were polymerase chain reaction (pcr) positive for babesia, and nucleotide sequencing revealed two distinct species or strains. two jackrabbi ... | 2006 | 16584322 |
| a study of the presence of b. burgdorferi, anaplasma (previously ehrlichia) phagocytophilum, rickettsia, and babesia in ixodes ricinus collected within the territory of belluno, italy. | in the years 2000 and 2001, we sampled ticks in order to establish the distribution of ixodes ricinus in the province of belluno; 5987 tick samples from 244 sites throughout the province were gathered, by dragging for a 5-min period. in 40 sites, seasonal variations and cycle stages of the parasites were studied at monthly intervals from march to september. a polymerase chain reaction (pcr) technique was used to identify the tick-infected sites. of 1931 individual ticks, 8.23% were positive for ... | 2006 | 16584324 |
| co-detection of bartonella henselae, borrelia burgdorferi, and anaplasma phagocytophilum in ixodes pacificus ticks from california, usa. | presence of bartonella dna was explored in 168 questing adult ixodes pacificus ticks from santa cruz county, california. bartonella henselae type i dna was amplified from 11 ticks (6.55%); previously, two (1.19%) were found to be infected with borrelia burgdorferi and five (2.98%) with anaplasma phagocytophilum. detection of b. henselae was not dependent on co-infection. the present study offers additional evidence that ixodes spp. ticks may act as hosts and possibly vectors for b. henselae. | 2006 | 16584332 |
| a reverse transcriptase-polymerase chain reaction assay of borrelia burgdorferi 16s rrna for highly sensitive quantification of pathogen load in a vector. | we developed a real-time quantitative detection assay for the pathogen borrelia burgdorferi, a lyme borreliosis (lb) agent, using reverse transcription-polymerase chain reaction (rt-pcr) with primers and probe for a borrelia genus-specific region of 16s ribosomal rna. the standard curve of the assay was linear by semi-log plot over more than five orders of magnitude, and the detection limit of the assay was one thousandth of a single cell of b. burgdorferi. the minimum target level for detection ... | 2006 | 16584333 |
| activity of lysosomal exoglycosidases in the serum of patients with chronic lyme arthritis. | to examine the role of lysosomal exoglycosidases in the pathogenesis of lyme arthritis we studied a group of 18 patients aged 18-72 (mean: 46 yr) diagnosed with chronic arthritis in the course of borreliosis. the control group was composed of 20 healthy volunteers (health service employees) aged 25-65 (mean: 45 yr) with no detectable serum anti-borrelia burgdorferi antibodies. we found that n-acetyl-beta-d-hexosaminidase (hex) was significantly increased and beta-galactosidase and alpha-mannosid ... | 2006 | 16584916 |
| antibiotic-refractory lyme arthritis is associated with hla-dr molecules that bind a borrelia burgdorferi peptide. | an association has previously been shown between antibiotic-refractory lyme arthritis, the human histocompatibility leukocyte antigen (hla)-dr4 molecule, and t cell recognition of an epitope of borrelia burgdorferi outer-surface protein a (ospa163-175). we studied the frequencies of hla-drb1-dqa1-dqb1 haplotypes in 121 patients with antibiotic-refractory or antibiotic-responsive lyme arthritis and correlated these frequencies with in vitro binding of the ospa163-175 peptide to 14 drb molecules. ... | 2006 | 16585267 |
| delineation of borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of borrelia spielmanii sp. nov. | to evaluate multilocus sequence analysis (mlsa) for taxonomic purposes in the delineation of species within borrelia burgdorferi sensu lato, seven relevant loci of various strains for which extensive dna-dna reassociation data were available were sequenced. mlsa delineation proved to be fully concordant with conventional methods. our analysis confirmed the delineation of a novel species, borrelia spielmanii sp. nov., previously known as 'borrelia spielmani' richter et al. 2004, with strain pc-eq ... | 2006 | 16585709 |
| [lichen sclerosus et atrophicus]. | a case of 43-year-old woman with skin and genital lesions of lichen sclerosus et atrophicus was presented. it was paid attention to simultaneously examination of the skin and genital areas. for this reason it is necessary closed cooperation between dermatologists and gynaecologists for determination of proper diagnosis. | 2005 | 16594486 |
| acvim small animal consensus statement on lyme disease in dogs: diagnosis, treatment, and prevention. | the purpose of this report is to offer a consensus opinion of acvim diplomates on the diagnosis, treatment, and prevention of borrelia burgdorferi infections in dogs (canine lyme disease). clinical syndromes known to commonly be associated with canine lyme disease include polyarthritis and glomerulopathy. serological test results can be used to document exposure to b. burgdorferi but not prove illness. although serum enzyme-linked immunosorbent assay/indirect fluorescent antibody assay titers ca ... | 2006 | 16594606 |
| physiological age of field-collected female taiga ticks, ixodes persulcatus (acari: ixodidae), and their infection with borrelia burgdorferi sensu lato. | in some studies the prevalence of tick infection (infection rate) and the intensity of infection are negatively correlated with unfed tick age (in the broad sense of this term). however, no special research has been carried out to consider the phenomenon thoroughly. the infection indices of the female taiga ticks, ixodes persulcatus, infected with borrelia burgdorferi s.l. were related to tick physiological age, an index that more precisely reflects tick physiological state than the time of tick ... | 2006 | 16596353 |
| prevalence of borrelia burgdorferi sensu lato and anaplasmataceae members in ixodes ricinus ticks in alsace, a focus of lyme borreliosis endemicity in france. | due to the high lyme borreliosis incidence in alsace, in northeastern france, we investigated in 2003-2004 three cantons in this region in order to determine the density of ixodes ricinus ticks infected by borrelia burgdorferi sensu lato and anaplasmataceae. the peak density of nymphs infected by b. burgdorferi sensu lato at munster and guebwiller, where the disease incidence was high, was among the highest reported in europe (105 and 114 per 100 m(2), respectively). in contrast, the peak densit ... | 2006 | 16598024 |
| anaplasma phagocytophilum-infected neutrophils enhance transmigration of borrelia burgdorferi across the human blood brain barrier in vitro. | the manifestations of lyme disease, caused by ixodes spp. tick-transmitted borrelia burgdorferi, range from skin infection to bloodstream invasion into the heart, joints and nervous system. the febrile infection human granulocytic anaplasmosis is caused by a neutrophilic rickettsia called anaplasma phagocytophilum, also transmitted by ixodes ticks. previous studies suggest that co-infection with a. phagocytophilum contributes to increased spirochetal loads and severity of lyme disease. however, ... | 2006 | 16600247 |
| preparation and purification of recombinant outer surface protein a (rospa) of borrelia burgdorferi sensu stricto and borrelia afzelii. | the recombinant outer surface protein a (rospa) from borrelia burgdorferi is a possible immunogen for protection of infected humans and animals against development of lyme borreliosis (lyme disease), a chronic tick-borne disease characterised by diverse dermatologic, neurologic, rheumatic, and cardiac manifestations. for several years, research and development have been directed towards a vaccine for the prevention of this debilitating disease. numerous animal studies demonstrate that pre-existi ... | 2005 | 16601765 |
| isolation and purification of recombinant outer surface protein c (rospc) of borrelia burgdorferi sensu lato. | the aim of this work was isolation and purification of the major immunodominant protein, outer surface protein c (ospc) of three members of the species group borrelia burgdorferi, the causative agent of lyme disease. our aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. for optimalization of protein purification's yield we used immobilized metal ion affinity chromatography (imac) under different conditions. the greatest efficiency was ac ... | 2005 | 16601766 |
| testing of the biocan b inj. ad us. vet. vaccine and development of the new recombinant vaccine against canine borreliosis. | verification of the efficacy of biocan b inj. ad us. vet. (bioveta, a.s.) was done by challenge testing. ticks collected in the nature were used as natural vectors of the infection. six beagles and two control ones were used in the test. formation of outer surface protein a specific antibodies (ospa antibodies) and borrelia specific immonoglobulins (igg) was measured by western blot and eia in the sera samples. the tissue samples were used for detection of borreliae by cultivation method and dar ... | 2005 | 16601776 |
| adhesion of borrelia garinii to neuronal cells is mediated by the interaction of ospa with proteoglycans. | to study pathogenic mechanisms of lyme meningoradiculitis, dorsal root ganglia (drg) cells and two neuronal cell lines (b50, sh-sy5y) were incubated with borrelia garinii, the borrelia species most frequently isolated from csf of lyme neuroborreliosis patients in europe. we demonstrated that (i) ospa-positive b. garinii adhere to neuronal cells, (ii) borrelia adhesion can be blocked by a monoclonal antibody against ospa, (iii) preincubation with proteoglycans interferes with the adhesion process ... | 2006 | 16603253 |
| a decrease in the immunoglobulin g antibody response against the vlse protein of borrelia burgdorferi sensu lato correlates with the resolution of clinical signs in antibiotic-treated patients with early lyme disease. | the purpose of this study was to evaluate the diagnostic performance of the liaison borrelia screen (diasorin, saluggia, italy), a new automated immunoassay based on the chemiluminescent technology (chemiluminescence immunoassay). to assess whether a decrease in a negative value in the anti-vlse immunoglobulin g (igg) antibody titer was correlated with a positive response to treatment, a group of serially collected serum samples from 67 patients with culture-confirmed erythema migrans was retros ... | 2006 | 16603623 |
| [lyme borreliosis: from the erythema migrans to lyme arthritis]. | an outbreak of arthritis (lyme arthritis) initiated the research leading to the description of the lyme disease in the years 1977-78 as a multisystemic tick-borne disease. five years later the spirochete responsible for lyme disease is discovered and will be named borrelia burgdorferi, in honor to its discoverer, dr willy burgdorfer. presently in europe, 6 borrelia species have been described and current knowledge on epidemiology is reported here. practical advises for the diagnostic of lyme art ... | 2006 | 16604874 |
| experimental infection of human body lice with acinetobacter baumannii. | the human body louse is currently recognized as a vector of rickettsia prowazekii, borrelia recurrentis, and bartonella quintana. previous studies have reported the isolation of acinetobacter baumannii from the body lice of homeless patients. to study how the body louse acquires a. baumannii, we infected a rabbit by infusing 2 x 10(6) colony-forming units of the louse strain of a. baumannii. two hundred body lice were infected by feeding on the bacteremic rabbit and compared with 200 uninfected ... | 2006 | 16606978 |
| a modest model explains the distribution and abundance of borrelia burgdorferi strains. | the distribution and abundance of borrelia burgdorferi, including human lyme disease strains, is a function of its interactions with vertebrate species. we present a mathematical model describing important ecologic interactions affecting the distribution and abundance of b. burgdorferi strains, marked by the allele at the outer surface protein c locus, in ixodes scapularis ticks, the principal vector. the frequency of each strain in ticks can be explained by the vertebrate species composition, t ... | 2006 | 16606995 |
| predicting density of ixodes pacificus nymphs in dense woodlands in mendocino county, california, based on geographic information systems and remote sensing versus field-derived data. | ixodes pacificus nymphs are the primary vectors to humans of borrelia burgdorferi, the etiologic agent of lyme disease, in california. we used a supervised classification model, based on remote sensing (rs) data from multi-seasonal landsat tm 5 images, to identify the key habitat in mendocino county where humans are exposed to i. pacificus nymphs (woodlands carpeted with leaf litter). the model, based on the normalized difference vegetation index (ndvi), brightness, and wetness, separated the ny ... | 2006 | 16606998 |
| [clinical polymorphism of neuroborreliosis at a late stage of the disease]. | 2006 | 16608111 | |
| [even in fever, joint pain and erythema: borrelia antibodies do not prove lyme disease]. | 2006 | 16612956 | |
| [concentrations of soluble factors participating in regulation of apoptosis of lymphocyte from patients with chronic lyme arthritis (preliminary report)]. | lyme borreliosis is a chronic infectious disease caused by borrelia burgdorferi spirochete. the excess and inadequate inflammatory response and even autoimmunity is considered to participate in its pathogenesis. apoptosis (programmed cell death) of activated lymphocytes contributes to regulation of immunological response and terminating the inflammation. | 2006 | 16617735 |
| residual brain infection in relapsing-fever borreliosis. | neurological involvement is common in the spirochetal infection relapsing fever (rf) in both humans and experimental animals. rf is best known for antigenic variation caused by the sequential expression of variable outer membrane lipoproteins of 2 sizes, variable small (vsp) and variable large (vlp) proteins. less understood is the persistence of rf borreliae in the brain after they are cleared from the blood, referred to as residual brain infection (rbi). our goal was to investigate the phenome ... | 2006 | 16619194 |
| borrelia lusitaniae and spotted fever group rickettsiae in ixodes ricinus (acari: ixodidae) in tuscany, central italy. | prevalence of infection by borrelia burgdorferi s.l. and spotted fever group (sfg) rickettsiae was estimated in host-seeking ticks in an area in tuscany, central italy, where lyme borreliosis was reported in a forestry worker. b. burgdorferi s.l. was identified by polymerase chain reaction in 16.7% (95% ci = 10.3, 24.8) of ixodes ricinus (l.) nymphs and 39.6% (95% ci = 26.5, 54.0) of adults. borrelia lusitaniae accounted for 82.9% of positive samples, followed by borrelia garinii (9.8%), borreli ... | 2006 | 16619594 |
| spatiotemporal patterns of host-seeking ixodes scapularis nymphs (acari: ixodidae) in the united states. | the risk of lyme disease for humans in the eastern united states is dependent on the density of host-seeking ixodes scapularis say nymphal stage ticks infected with borrelia burgdorferi. although many local and regional studies have estimated lyme disease risk using these parameters, this is the first large-scale study using a standardized methodology. density of host-seeking i. scapularis nymphs was measured by drag sampling of closed canopy deciduous forest habitats in 95 locations spaced amon ... | 2006 | 16619595 |
| an avian contribution to the presence of ixodes pacificus (acari: ixodidae) and borrelia burgdorferi on the sutter buttes of california. | birds from 45 species were sampled during three spring seasons from an isolated canyon on the sutter buttes in california for the presence of subadult stages of ixodes pacificus cooley & kohls, and for infection with borrelia burgdorferi johnson, schmid, hyde, steigerwalt & brenner. these birds were found to have an infestation prevalence of 45%, a density of 1.7 ticks per bird, and an intensity of 3.8 ticks per infested bird. there was a significant difference in the i. pacificus infestations b ... | 2006 | 16619623 |
| geographical distribution patterns and habitat suitability models for presence of host-seeking ixodid ticks in dense woodlands of mendocino county, california. | we used drag sampling to examine the geographical distribution patterns of ixodid ticks engaging in open (non-nidicolous) host-seeking behavior in dense woodland habitats of the climatically and ecologically diverse mendocino county in north coastal california. the findings based on this sampling methodology reflect risk of human exposure to host-seeking ticks rather than the true distribution of the ticks. drag sampling in 78 sites yielded 7,860 nymphal or adult ixodes pacificus cooley & kohls, ... | 2006 | 16619628 |
| hepatitis b virus capsid-like particles can display the complete, dimeric outer surface protein c and stimulate production of protective antibody responses against borrelia burgdorferi infection. | hepatitis b virus capsid-like particles (clps), icosahedral assemblies formed by 90 or 120 core protein dimers, hold promise as immune-enhancing vaccine carriers for heterologous antigens. insertions into the immunodominant c/e1 b cell epitope, a surface-exposed loop, are especially immunogenic. however, display of whole proteins, desirable to induce multispecific and possibly neutralizing antibody responses, can be restrained by an unsuitable structure of the foreign protein and by its propensi ... | 2006 | 16621801 |
| borrelia burgdorferi ftsz plays a role in cell division. | 2006 | 16621841 | |
| bgp, a secreted glycosaminoglycan-binding protein of borrelia burgdorferi strain n40, displays nucleosidase activity and is not essential for infection of immunodeficient mice. | bgp, one of the surface-localized glycosaminoglycan-binding proteins of the lyme disease spirochete, borrelia burgdorferi, exhibited nucleosidase activity. infection of scid mice with b. burgdorferi strain n40 mutants harboring a targeted insertion in bgp and apparently retaining all endogenous plasmids revealed that bgp is not essential for colonization of immunocompromised mice. | 2006 | 16622242 |
| evidence that the bba68 protein (bbcrasp-1) of the lyme disease spirochetes does not contribute to factor h-mediated immune evasion in humans and other animals. | bba68 (bbcrasp-1) of the lyme disease spirochetes binds human factor h (fh) and fh-like protein 1 (fhl-1). here we assess transcription of the bba68 gene and production of bba68 in infected mice and humans using real-time reverse transcriptase pcr and immunoblotting. the species specificity of fh binding to bba68 was also tested. the data suggest that bba68 does not play an important role in immune evasion in animals. | 2006 | 16622245 |
| molecular analysis of microbial communities identified in different developmental stages of ixodes scapularis ticks from westchester and dutchess counties, new york. | ixodes scapularis ticks play an important role in the transmission of a wide variety of pathogens between various mammalian species, including humans. pathogens transmitted by ticks include borrelia, anaplasma and babesia. although ticks may harbour both pathogenic and non-pathogenic microflora, little is known about how the diversity of the microflora within ticks may influence the transmission of pathogens. to begin addressing this question, we examined the composition of bacterial communities ... | 2006 | 16623735 |
| immunodominant epitope in the c-terminus of a variable major protein in borrelia duttonii, an agent of tick-borne relapsing fever. | borrelia duttonii strain ly was isolated from a child with tick-borne relapsing fever in tanzania. b. duttonii produces variable major proteins (vmps), which undergo antigenic variation. we previously reported transcription of the vmpp gene, which is one of the vmp genes in strain ly, detected in vitro cultivation. in the current study, we purified the recombinant non-lipidated vmpp protein by affinity chromatography and produced vmpp polyclonal antibodies. antigenicity of vmpp was examined by w ... | 2006 | 16625051 |
| relapsing fever spirochaetes produce a serine protease that provides resistance to oxidative stress and killing by neutrophils. | the spirochaetes that cause tick-borne relapsing fever and lyme disease are closely related human pathogens, yet they differ significantly in their ecology and pathogenicity. genome sequencing of two species of relapsing fever spirochaetes, borrelia hermsii and borrelia turicatae, identified a chromosomal open reading frame, designated bhpa, not present in the lyme disease spirochaete borrelia burgdorferi. the predicted amino acid sequence of bhpa was homologous with the htra serine proteases, w ... | 2006 | 16629672 |
| neuroborreliosis and the pediatric population: a review. | to review the medical literature on neuroborreliosis, in particular its clinical features in both adults and children, and highlight the differences between the two groups, with an emphasis on the pediatric population. | 2006 | 16642458 |
| serological evidence of rickettsia infections in forestry rangers in north-eastern italy. | the prevalence of antibodies to rickettsiae and other tick-borne microrganisms in the sera of 181 forestry rangers from friuli-venezia-giulia, italy, was examined. seven (3.9%) sera were positive for rickettsia conorii and rickettsia helvetica, as single or dual infections; four of these sera had been found previously to be positive for borrelia burgdorferi. antibodies to coxiella burnetii were detected in five (2.8%) sera, four of which were also positive for b. burgdorferi. these findings indi ... | 2006 | 16643531 |
| vlse c6 peptide and igg elisa antibody analysis for clinical diagnosis of lyme borreliosis in an endemic area. | 2006 | 16643532 | |
| the ticking time bomb. | a man with orbital myositis and optic neuritis tested positive serologically for multiple tick-borne diseases. erlichiosis, babesiosis, and lyme disease may occur together and affect the eye or orbit. | 2006 | 16644367 |
| atovaquone plus cholestyramine in patients coinfected with babesia microti and borrelia burgdorferi refractory to other treatment. | ten percent of us patients with lyme disease are coinfected with babesia microti. a double-blind, placebo-controlled, crossover trial enrolled 25 patients with confirmed borrelia burgdorferi/b microti coinfection, abnormal visual contrast sensitivity (vcs), and persistent symptoms despite prior treatment with atovaquone and azithromycin. patients were randomly assigned to atovaquone suspension or placebo plus cholestyramine for 3 weeks, were crossed over for 3 weeks, and then received open-label ... | 2006 | 16644602 |
| [borreliosis--increasing clinical problem]. | lyme disease (ld) is due to infection with borrelia burgdorferi (b. burgdorferi). we analysed some aspects of epidemiology and clinical manifestation of borreliosis. we tried to estimate the efficiency of diagnostic methods and treatment. we analyzed medical documentation of 300 patients with ld treated in our department between 1993-2001. the diagnosis was made according to lyme disease foundation's criteria. patients suffering from ld were divided into 3 groups according to stages of the disea ... | 2006 | 16646287 |
| climate, deer, rodents, and acorns as determinants of variation in lyme-disease risk. | risk of human exposure to vector-borne zoonotic pathogens is a function of the abundance and infection prevalence of vectors. we assessed the determinants of lyme-disease risk (density and borrelia burgdorferi-infection prevalence of nymphal ixodes scapularis ticks) over 13 y on several field plots within eastern deciduous forests in the epicenter of us lyme disease (dutchess county, new york). we used a model comparison approach to simultaneously test the importance of ambient growing-season te ... | 2006 | 16669698 |
| plaques of alzheimer's disease originate from cysts of borrelia burgdorferi, the lyme disease spirochete. | here is hypothesized a truly revolutionary notion that rounded cystic forms of borrelia burgdorferi are the root cause of the rounded structures called plaques in the alzheimer brain. rounded "plaques' in high density in brain tissue are emblematic of alzheimer's disease (ad). plaques may be conceptualized as rounded "pock mark-like" areas of brain tissue injury. in this century, in brain tissue of ad, plaques are amyloid plaques according to the most up to date textbooks. in the last century, h ... | 2006 | 16675154 |
| a single-run, real-time pcr for detection and identification of borrelia burgdorferi sensu lato species, based on the hbb gene sequence. | lyme borreliosis is the most important vector-borne disease caused by spirochetes within the borrelia burgdorferi sensu lato (b. burgdorferi sl) complex. there is strong evidence that different species of this group of genetically diverse spirochetes are involved in distinct clinical manifestations of the disease. in order to differentiate species within this bacterial complex, we developed a real-time-pcr protocol, which targets the hbb gene. we designed a fluorescein-labeled probe specific of ... | 2006 | 16684099 |
| co-evolution of the outer surface protein c gene (ospc) and intraspecific lineages of borrelia burgdorferi sensu stricto in the northeastern united states. | clinical and tick isolates of borrelia burgdorferi sensu stricto, the bacterial agent of lyme disease, from the northeastern united states were sequenced at 12 loci located on the main chromosome and 7 plasmids (lp54, cp26, cp9, lp17, lp25, lp28-2, and lp38). the outer surface protein c gene (ospc) showed the highest number (12) of major alleles (defined as alleles differing by 5% or more in nucleotide sequence) while other orfs had only two to four major alleles. a non-recombining chromosomal m ... | 2007 | 16684623 |
| improved serodiagnosis of lyme disease. | aims-to determine whether enzyme linked immunosorbent assay (elisa) results for borrelia burgdorferi require confirmation by immunoblotting and how immunoblotting may best be used in the diagnosis of lyme disease.methods-over one year, all referrals for lyme disease to a district general hospital with a large tick population in its catchment area were tested by elisa. positive, low positive and negative serum samples were subjected to immunoblotting and the reactive bands analysed.results-in tot ... | 1996 | 16696055 |
| borrelia burgdorferi--traveling incognito? | we outline in this review how borrelia burgdorferi, the causative agent of lyme disease, moves from the tick to the vertebrate host, and what molecules are potentially involved in this challenging commute. the survival strategies utilized by the spirochete during transmission and the initial stages of infection are discussed. | 2006 | 16698304 |
| a paretic condition in an anaplasma phagocytophilum infected roe deer calf. | this paper describes a case of anaplasma phagocytophilum infection in a roe deer (capreolus capreolus) calf in norway. the calf was found deserted, paretic, and heavily infested with ixodes ricinus ticks. it was euthanized and investigated postmortem. anaplasma phagocytophilum was detected in several tissues by polymerase chain reaction (pcr) and 16s rrna sequence analyses. analyses for borrelia burgdorferi sensu lato and tick-borne encephalitis (tbe) virus infections were negative. this is the ... | 2006 | 16699161 |
| occurrence of ticks infected by tickborne encephalitis virus and borrelia genospecies in mountains of the czech republic. | 2005 | 16702647 | |
| neuroborreliosis in an hiv-1 positive patient. | simultaneous co-infections of borrelia burgdorferi sensu lato and hiv-1 are rare events, with only six published cases. a case of acute neuroborreliosis with facial palsy, meningoradiculitis (bannwarth's syndrome) in an hiv-1 positive individual is described. diagnosis was confirmed by western immunoblot analysis of serum and csf and by proof of intrathecal production of antibodies against b. garinii. the patient was successfully treated with cefotaxime. in all published hiv+ cases, the course o ... | 2006 | 16703302 |
| possibilities for relapsing fever reemergence. | relapsing fever borrelia infections have attracted little attention in recent years; however, where endemic, these infections still result in considerable illness and death. despite the marked antimicrobial drug susceptibility of these organisms, therapy is often delayed through lack of clinical suspicion. with increasing travel, infections may be imported, through exotic relapsing fever infection or through resurgence of infected disease vectors. although louseborne relapsing fever is now geogr ... | 2006 | 16704771 |
| epidemic spread of lyme borreliosis, northeastern united states. | we examined the degree of host specialization of different strains of borrelia burgdorferi, the tickborne pathogen that causes lyme borreliosis in the northeastern united states. we first assessed the genetic population structures of b. burgdorferi in ticks obtained from different mammalian host species and in questing ticks sampled in a woodland ecosystem in connecticut. by comparing the patterns found in our study with data from another cross-sectional study, we demonstrate that b. burgdorferi ... | 2006 | 16704808 |
| the interaction of bacterial pathogens with platelets. | in recent years, the frequency of serious cardiovascular infections such as endocarditis has increased, particularly in association with nosocomially acquired antibiotic-resistant pathogens. growing evidence suggests a crucial role for the interaction of bacteria with human platelets in the pathogenesis of cardiovascular infections. here, we review the nature of the interactions between platelets and bacteria, and the role of these interactions in the pathogenesis of endocarditis and other cardi ... | 2006 | 16710325 |
| borrelia burgdorferi lacking bbk32, a fibronectin-binding protein, retains full pathogenicity. | bbk32, a fibronectin-binding protein of borrelia burgdorferi, is one of many surface lipoproteins that are differentially expressed by the lyme disease spirochete at various stages of its life cycle. the level of bbk32 expression in b. burgdorferi is highest during infection of the mammalian host and lowest in flat ticks. this temporal expression profile, along with its fibronectin-binding activity, strongly suggests that bbk32 may play an important role in lyme pathogenesis in the host. to test ... | 2006 | 16714558 |
| delineating the requirement for the borrelia burgdorferi virulence factor ospc in the mammalian host. | we previously demonstrated that outer surface protein c (ospc) of borrelia burgdorferi is essential for establishing mammalian infection. however, the role of ospc in mammalian infection is unknown. here, we report experiments designed to distinguish between two models of ospc function in the mammalian host: (i) ospc fulfills an essential physiological role for growth and host adaptation or (ii) ospc provides a protective role for evasion of components of the innate immune response. we found tha ... | 2006 | 16714587 |