Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| prevalence of entamoeba histolytica/entamoeba dispar in the city of campina grande, in northeastern brazil. | there is a clear need to perform epidemiological studies to find the true prevalence of entamoeba histolytica around the world. the evaluation of this prevalence has been hindered by the existence of two different species which are morphologically identical, but genetically different, namely e. histolytica, which causes amebiasis, and e. dispar, which is non-pathogenic. in brazil, the e. dispar has been detected in communities in the southeastern (se) and northeastern (ne) regions with poor sani ... | 2015 | 25229229 |
| amoebic pi3k and pkc is required for jurkat t cell death induced by entamoeba histolytica. | the enteric protozoan parasite entamoeba histolytica is the causative agent of human amebiasis. during infection, adherence of e. histolytica through gal/galnac lectin on the surface of the amoeba can induce caspase-3-dependent or -independent host cell death. phosphorylinositol 3-kinase (pi3k) and protein kinase c (pkc) in e. histolytica play an important function in the adhesion, killing, or phagocytosis of target cells. in this study, we examined the role of amoebic pi3k and pkc in amoeba-ind ... | 2014 | 25246714 |
| the mak16 gene of entamoeba histolytica and its identification in isolates from patients. | to identify sequences of entamoeba histolytica associated with the development of amebic liver abscess (ala) in hamsters, subtractive hybridization of cdna from e. histolytica hm-1:imss under 2 growth conditions was performed: 1) cultured in axenic medium and 2) isolated from experimental ala in hamsters. for this procedure, 6 sequences were obtained. of these sequences, the mak16 gene was selected for amplification in 29 cultures of e. histolytica isolated from the feces of 10 patients with int ... | 2014 | 25246723 |
| neglected tropical diseases among two indigenous subtribes in peninsular malaysia: highlighting differences and co-infection of helminthiasis and sarcocystosis. | soil-transmitted helminth (sth) infections have been documented among these minority groups since 1938. however the prevalence of sth is still high among these communities. most studies tend to consider the orang asli (indigenous) as a homogenous group. in contrary, different subtribes have their own cultural practices. to understand this variation better, we studied the prevalence and associated factors of sth and other gut parasitic infections among two common subtribes (i.e. temuan and temiar ... | 2014 | 25248116 |
| cultivation of parasites. | parasite cultivation techniques constitute a substantial segment of present-day study of parasites, especially of protozoa. success in establishing in vitro and in vivo culture of parasites not only allows their physiology, behavior and metabolism to be studied dynamically, but also allows the nature of the antigenic molecules in the excretory and secretory products to be vigorously pursued and analyzed. the complex life-cycles of various parasites having different stages and host species requir ... | 0 | 25250227 |
| laboratory methods of identification of entamoeba histolytica and its differentiation from look-alike entamoeba spp. | entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis, is a common parasitic cause of significant morbidity and mortality in the developing countries. hence, early detection and differentiation of pathogenic e. histolytica from nonpathogenic/commensal entamoeba spp (entamoeba dispar/entamoeba moshkovskii/entamoeba bangladeshi) plays a crucial role in clinical management of patients with amebiasis. most diagnostic tests currently available do not reliably differen ... | 2014 | 25250228 |
| entamoeba bangladeshi: an insight. | molecular tools have the potential to differentiate microscopically similar gut micro-eukaryotes that may have significantly different relationships with the human host. using broad range entamoeba primers to amplify a section of the eukaryotic 18s small subunit ribosomal rna gene a novel member of the entamoeba family (entamoeba bangladeshi) has recently been identified. the goal of this review is to place this species in the context of what is already known about this genus and to discuss the ... | 2014 | 25250229 |
| amoeboma of colon mimicking colonic carcinoma. | entamoeba histolytica is a major cause of diarrhea in the developing countries and it can present with a wide range of gastrointestinal symptoms depending on the phase of the infection. we describe a case of 65-year-old male patient presented with abdominal pain and vomiting. on the clinical and radiological examination provisional diagnosis of colonic carcinoma was suspected. after resection of perforated caecal growth, histopathological sections showed numerous trophozoites of e. histolytica i ... | 2014 | 25250235 |
| microscopy versus enzyme linked immunosorbent assay test for detection of entamoeba histolytica infection in stool samples. | 2014 | 25250240 | |
| interaction between nbp35 and cfd1 proteins of cytosolic fe-s cluster assembly reveals a stable complex formation in entamoeba histolytica. | iron-sulfur (fe-s) proteins are involved in many biological functions such as electron transport, photosynthesis, regulation of gene expression and enzymatic activities. biosynthesis and transfer of fe-s clusters depend on fe-s clusters assembly processes such as isc, suf, nif, and cia systems. unlike other eukaryotes which possess isc and cia systems, amitochondriate entamoeba histolytica has retained nif & cia systems for fe-s cluster assembly in the cytosol. in the present study, we have eluc ... | 2014 | 25271645 |
| the parasite entamoeba histolytica exploits the activities of human matrix metalloproteinases to invade colonic tissue. | intestinal invasion by the protozoan parasite entamoeba histolytica is characterized by remodelling of the extracellular matrix (ecm). the parasite cysteine proteinase a5 (cp-a5) is thought to cooperate with human matrix metalloproteinases (mmps) involved in ecm degradation. here, we investigate the role cp-a5 plays in the regulation of mmps upon mucosal invasion. we use human colon explants to determine whether cp-a5 activates human mmps. inhibition of the mmps' proteolytic activities abolishes ... | 2014 | 25291063 |
| crystal structures and kinetics of type iii 3-phosphoglycerate dehydrogenase reveal catalysis by lysine. | d-phosphoglycerate dehydrogenase (pgdh) catalyzes the first committed step of the phosphorylated serine biosynthesis pathway. here, we report for the first time, the crystal structures of type iiik pgdh from entamoeba histolytica in the apo form, as well as in complexes with substrate (3-phosphoglyceric acid) and cofactor (nad(+) ) to 2.45, 1.8 and 2.2 å resolution, respectively. comparison of the apo structure with the substrate-bound structure shows that the substrate-binding domain is rotated ... | 2014 | 25294608 |
| prevalent and incident hiv diagnoses among entamoeba histolytica-infected adult males: a changing epidemiology associated with sexual transmission--taiwan, 2006-2013. | sexually transmitted entamoeba histolytica infection (ehi) has been increasingly recognized among men who have sex with men (msm). we used the national disease surveillance systems (ndss) to identify prevalent and incident hiv diagnoses among adults with ehi and to determine the associated factors. | 2014 | 25299178 |
| a novel alpha kinase ehak1 phosphorylates actin and regulates phagocytosis in entamoeba histolytica. | phagocytosis plays a key role in nutrient uptake and virulence of the protist parasite entamoeba histolytica. phagosomes have been characterized by proteomics, and their maturation in the cells has been studied. however, there is so far not much understanding about initiation of phagocytosis and formation of phagosomes at the molecular level. our group has been studying initiation of phagocytosis and formation of phagosomes in e. histolytica, and have described some of the molecules that play ke ... | 2014 | 25299184 |
| biochemical and kinetic characterization of the recombinant adp-forming acetyl coenzyme a synthetase from the amitochondriate protozoan entamoeba histolytica. | entamoeba histolytica, an amitochondriate protozoan parasite that relies on glycolysis as a key pathway for atp generation, has developed a unique extended ppi-dependent glycolytic pathway in which adp-forming acetyl-coenzyme a (coa) synthetase (acd; acetate:coa ligase [adp-forming]; ec 6.2.1.13) converts acetyl-coa to acetate to produce additional atp and recycle coa. we characterized the recombinant e. histolytica acd and found that the enzyme is bidirectional, allowing it to potentially play ... | 2014 | 25303954 |
| [analysis of intestinal ameobiasis in patients with diarrhea by adhesin antigen test and direct microscopy]. | in this study, we aimed to research the frequency of intestinal amebiasis in patients who applied with diarrhea retrospectively for a year and compare direct microscopic analysis and elisa adhesin antigen test for entamoeba histolytica procedures. | 2014 | 25308451 |
| differentially expressed genes of virulent and nonvirulent entamoeba histolytica strains identified by suppression subtractive hybridization. | entamoeba histolytica is a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. as this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. using the suppression subtractive hybridization (ssh) technique, differential gene expressions of two e. histolytica strains, one virulent (egg) and one nonvirulent (452), have been analyzed with the purpose of isolating genes which may be inv ... | 2014 | 25313356 |
| colonoscopic diagnosis of amebiasis: a case series and systematic review. | nonspecific gastrointestinal symptoms make diagnosis of amebiasis difficult. certain colonoscopic findings predict amebic colitis while others suggest different diagnoses. we aimed to evaluate the diagnostic capability of colonic evaluation of amebiasis. | 2015 | 25346004 |
| in vivo identification of the steps that control energy metabolism and survival of entamoeba histolytica. | the steps that control the entamoeba histolytica glycolytic flux were here identified by elasticity analysis, an experimental approach of metabolic control analysis. the concentrations of glycolytic metabolites were gradually varied in live trophozoites by (a) feeding with different glucose concentrations and (b) inhibiting the final pathway steps; in parallel, the changes in the pathway flux were determined. from the metabolite concentration-flux relationship, the elasticity coefficients of ind ... | 2015 | 25350227 |
| degradation of the transcription factors nf-κb, stat3, and stat5 is involved in entamoeba histolytica-induced cell death in caco-2 colonic epithelial cells. | entamoeba histolytica is a tissue-invasive protozoan parasite causing dysentery in humans. during infection of colonic tissues, amoebic trophozoites are able to kill host cells via apoptosis or necrosis, both of which trigger il-8-mediated acute inflammatory responses. however, the signaling pathways involved in host cell death induced by e. histolytica have not yet been fully defined. in this study, we examined whether calpain plays a role in the cleavage of pro-survival transcription factors d ... | 2014 | 25352693 |
| entamoeba histolytica meningoencephalitis diagnosed by trophozoites in cerebrospinal fluid. | entamoeba histolytica meningoencephalitis has not been described in the modern literature, which is distinct from that caused by free-living amoebae. we report the first case of e. histolytica meningoencephalitis without liver or brain abscesses. cerebrospinal fluid revealed 2 + very motile trophozoites. our patient was successfully treated with intravenous metronidazole. | 2013 | 25356319 |
| bone marrow dendritic cells from mice with an altered microbiota provide interleukin 17a-dependent protection against entamoeba histolytica colitis. | there is an emerging paradigm that the human microbiome is central to many aspects of health and may have a role in preventing enteric infection. entamoeba histolytica is a major cause of amebic diarrhea in developing countries. it colonizes the colon lumen in close proximity to the gut microbiota. interestingly, not all individuals are equally susceptible to e. histolytica infection. therefore, as the microbiota is highly variable within individuals, we sought to determine if a component of the ... | 2014 | 25370489 |
| mass spectrometric analysis of l-cysteine metabolism: physiological role and fate of l-cysteine in the enteric protozoan parasite entamoeba histolytica. | l-cysteine is essential for virtually all living organisms, from bacteria to higher eukaryotes. besides having a role in the synthesis of virtually all proteins and of taurine, cysteamine, glutathione, and other redox-regulating proteins, l-cysteine has important functions under anaerobic/microaerophilic conditions. in anaerobic or microaerophilic protozoan parasites, such as entamoeba histolytica, l-cysteine has been implicated in growth, attachment, survival, and protection from oxidative stre ... | 2014 | 25370494 |
| structure-based identification of inositol polyphosphate 1-phosphatase from entamoeba histolytica. | inositol polyphosphate 1-phosphatase from entamoeba histolytica (ehippase) is an mg(2+)-dependent and li(+)-sensitive enzyme that catalyzes the hydrolysis of inositol 1,4-bisphosphate [ins(1,4)p2] into myo-inositol 1-monophosphate and po4(3-). in the present work, ehippase has been biochemically identified and its crystal structure has been determined in the presence of mg(2+) and po4(3-) at 2.5 å resolution. this enzyme was previously classified as a 3'(2'),5'-bisphosphate nucleotidase in the n ... | 2014 | 25372691 |
| prevalence of cryptosporidiosis in renal transplant recipients presenting with acute diarrhea at a single center in pakistan. | cryptosporidium is an intracellular protozoan organism which causes diarrhea, both in immunocompetent and immunocompromised hosts. renal transplant recipients are prone to develop a variety of infections including protozoal infections. | 2014 | 25374881 |
| intestinal parasitic diarrhea among children in baghdad--iraq. | parasitic diarrhea among children is a significant health problem worldwide. this cross sectional study described the burden of parasitic diarrhea among children. the objectives of this study were to evaluate the impact of risk factors on the parasitic diarrhea, and to determine the parasitic profile among children in baghdad-iraq, during the period extending from september 2003 to june 2004. a total number of 2033 cases were included in the study. the estimated prevalence rate of parasitic diar ... | 2014 | 25382477 |
| headache following occipital brain lesion: a case of migraine triggered by occipital spikes? | this study describes the case of an 8-year-old boy who developed a genuine migraine after the surgical excision, from the right occipital lobe, of brain abscesses due to selective infestation of the cerebrum by entamoeba histolytica. after the surgical treatment, the boy presented daily headaches with typical migraine features, including right-side parieto-temporal pain, nausea, vomiting, and photophobia. electroencephalography (eeg) showed epileptiform discharges in the right occipital lobe, al ... | 2015 | 25406125 |
| [the current view of the diagnosis and management of amebiasis in the light of the authors own case reports]. | this comprehensive review is focused on a serious protozoan disease, amebiasis. this disease is caused by the human parasite entamoeba histolytica (e. histolytica), the second leading cause of mortality due to protozoan disease worldwide (the leading cause is malaria). the incidence of amebiasis in the czech republic is very low, but it may be underreported as the disease often escapes diagnosis. intestinal colonisation by e. histolytica may be asymptomatic. the clinical picture ranges from diar ... | 2014 | 25412488 |
| [entamoeba histolytica: erythrocyt e phagocytosis and capture distance]. | 2016 | 25418666 | |
| prevalence of intestinal parasites among hiv patients at the yaoundé central hospital, cameroon. | intestinal parasites are more common in people with hiv, especially in tropical developing countries. this cross-sectional study was carried out to assess the prevalence of intestinal parasites among people with hiv at the yaoundé central hospital accredited treatment centre. | 2014 | 25419274 |
| immune markers characteristic for asymptomatically infected and diseased entamoeba histolytica individuals and their relation to sex. | the protozoan parasite entamoeba histolytica (e. histolytica) usually asymptomatically colonizes the human intestine. in the minority of the cases, the parasite evades from the gut and can induce severe symptoms like colitis or amebic liver abscess (ala). interestingly, ala predominates in adult men despite a higher prevalence of the parasite in women. the present study aimed to identify characteristic serum markers in a unique cohort of clearly defined asymptomatically infected e. histolytica i ... | 2014 | 25420932 |
| a comparative survey of the prevalence of human parasites found in fresh vegetables sold in supermarkets and open-aired markets in accra, ghana. | consuming raw vegetables offers essential nutrients that one may not get when such vegetables are usually cooked. however, eating them raw may pose a great risk for transmissions of pathogens. such risks may be influenced by the sources of the vegetables and washing techniques used. the aim of the study was to compare the prevalence and diversity of parasitic pathogens associated with vegetables sold at the two types of markets in ghana and compare effectiveness of various washing techniques. | 2014 | 25424391 |
| prevalence and its associated risk factors of intestinal parasitic infections among yadot primary school children of south eastern ethiopia: a cross-sectional study. | intestinal parasitic infections are posing significant morbidity worldwide. in ethiopia, due to poor socio-economic status, intestinal parasitic infections are highly prevalent. the main aim of this study was to determine the prevalence of intestinal parasites and its associated risk factors among yadot primary school children which is found in south-eastern part of ethiopia, in the district called delo-mena. | 2014 | 25425173 |
| multisite clinical evaluation of a rapid test for entamoeba histolytica in stool. | rapid point-of-care detection of enteric protozoa in diarrheal stool is desirable in clinical and research settings to efficiently determine the etiology of diarrhea. we analyzed the ability of the third-generation e. histolytica quik chek assay developed by techlab to detect amebic antigens in fecal samples collected from independent study populations in south africa and bangladesh. we compared the performance of this recently released rapid test to that of the commercially available prospect e ... | 2015 | 25428152 |
| crystal structures of entamoeba histolytica lysyl-trna synthetase reveal conformational changes upon lysine binding and a specific helix bundle domain. | the class ii lysyl-trna synthetases (krs) are conserved aminoacyl-trna synthetases that attach lysine to the cognate trna in a two-step mechanism. the enzyme from the parasitic protozoan entamoeba histolytica was crystallized in the presence of small ligands to generate snapshots of the lysine-adenylate formation. the residues involved in lysine activation are highly conserved and the active site closes around the lysyl-adenylate, as observed in bacterial krs. the entamoeba emapii-like polypepti ... | 2014 | 25448989 |
| immunization with the entamoeba histolytica surface metalloprotease ehmsp-1 protects hamsters from amebic liver abscess. | diarrhea and amebic liver abscesses due to invasive entamoeba histolytica infections are an important cause of morbidity and mortality in the developing world. entamoeba histolytica adherence and cell migration, two phenotypes linked to virulence, are both aberrant in trophozoites deficient in the metallosurface protease ehmsp-1, which is a homologue of the leishmania vaccine candidate leishmanolysin (gp63). we examined the potential of ehmsp-1 for use as a vaccine antigen to protect against ame ... | 2015 | 25452550 |
| [colonic perforation due to invasive amebic colitis during anti-tnf therapy for spondyloarthritis]. | tnf blockade has been successful in the treatment of some rheumatic diseases such as spondyloarthritis. many infectious complications have been reported with anti-tnf therapy, mainly bacterial, mycobacterial, viral and fungal infections. entamoeba histolytica is an extracellular protozoan parasite that mainly causes colitis and hepatic abscess; bowel perforation is an uncommon complication with high mortality. tnf is considered the principal mediator of cell immunity against amebiasis. initially ... | 2016 | 25458030 |
| amebiasis presenting as acute appendicitis: report of a case and review of japanese literature. | outside of these high-risk regions, acute amebic appendicitis is considerably rarer and the mortality rate is much higher than with non-amebic appendicitis. | 2014 | 25460473 |
| infectious diseases in immigrant population related to the time of residence in spain. | the aim of this study was to evaluate the data on the main imported infectious diseases and public health issues arising from the risk of transmission of tropical and common diseases in the immigrant population. during the period of study, 2,426 immigrants were attended in the tropical medicine unit of the hospital of poniente. for each patient, a complete screening for common and tropical diseases was performed. the prevalence and main features of intestinal and urinary parasites, microfilarias ... | 2016 | 25466580 |
| ascaris spp. and capillaria caudinflata infections in captive-bred crested ibis (nipponia nippon) in china. | crested ibis (nipponia nippon), an endan gered native bird, was called the "precious stone" of oriental birds. n. nippon was considered a critically endangered species in the iucn red list of threatened species and a first-class national protected animal in china. the chinese government had exerted considerable effort to protect the n. nippon population. an effective approach to increase the number of these birds was captive breeding. however, several pathogens, including parasites, could jeopar ... | 2015 | 25486916 |
| regulation of serum-responsive transmembrane kinase ehtmkb1-9 by an unsaturated lipid, oleic acid in protistan parasite entamoeba histolytica. | transmembrane kinases of entamoeba histolytica are known to play a wide range of roles from virulence, phagocytosis, and proliferation to stress response. transmembrane kinase ehtmkb1-9 is thought to be involved in early proliferative response and it was originally identified as a serum inducible gene. ability to stimulate ehtmkb1 expression of serum starved cells resides in unsaturated fatty acids associated with albumin fraction of serum and the mechanism of stimulation follows activation of e ... | 2014 | 25497959 |
| modulation of endogenous cysteine protease inhibitor (icp) 1 expression in entamoeba histolytica affects amoebic adhesion to extracellular matrix proteins. | entamoeba histolytica is an enteric tissue-invading protozoan parasite that causes amoebic colitis and occasionally liver abscess in humans. during tissue invasion, amoebic adhesion to host components is an important event for host cell death leading to successful invasion and infection. among amoebic virulence factors, gal/galnac lectin is known to be major adhesion factor to host cells. in this study, we investigated the role of amoebic secreted cp (cysteine proteases) in amoebic adhesion to e ... | 2015 | 25500214 |
| crystal structure of calcium binding protein-5 from entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes. | entamoeba histolytica is the etiological agent of human amoebic colitis and liver abscess, and causes a high level of morbidity and mortality worldwide, particularly in developing countries. there are a number of studies that have shown a crucial role for ca2+ and its binding protein in amoebic biology. ehcabp5 is one of the ef hand calcium-binding proteins of e. histolytica. we have determined the crystal structure of ehcabp5 at 1.9 å resolution in the ca2+-bound state, which shows an unconvent ... | 2014 | 25502654 |
| evolutionary genomics and population structure of entamoeba histolytica. | amoebiasis caused by the gastrointestinal parasite entamoeba histolytica has diverse disease outcomes. study of genome and evolution of this fascinating parasite will help us to understand the basis of its virulence and explain why, when and how it causes diseases. in this review, we have summarized current knowledge regarding evolutionary genomics of e. histolytica and discussed their association with parasite phenotypes and its differential pathogenic behavior. how genetic diversity reveals pa ... | 2014 | 25505504 |
| leptin receptor mutation results in defective neutrophil recruitment to the colon during entamoeba histolytica infection. | amebiasis is an enteric infection caused by entamoeba histolytica, with symptoms ranging in severity from asymptomatic colonization to dysentery. humans with the q223r leptin receptor mutation have increased susceptibility to amebiasis, but the mechanism has been unclear. using a mouse model expressing the mutation, we tested the impact of the q223r mutation on the innate immune response to e. histolytica infection. the 223r mutation resulted in delayed clearance of amebae from the cecum, as had ... | 2014 | 25516614 |
| antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis. | amoebiasis caused by entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. we collected 32 plants used in northeast mex ... | 2014 | 25517343 |
| entamoeba histolytica racc selectively engages p21-activated kinase effectors. | rho family gtpases modulate actin cytoskeleton dynamics by signaling through multiple effectors, including the p21-activated kinases (paks). the intestinal parasite entamoeba histolytica expresses ∼20 rho family gtpases and seven isoforms of pak, two of which have been implicated in pathogenesis-related processes such as amoebic motility and invasion and host cell phagocytosis. here, we describe two previously unstudied pak isoforms, ehpak4 and ehpak5, as highly specific effectors of ehracc. a s ... | 2015 | 25529118 |
| copro prevalence and estimated risk of entamoeba histolytica in diarrheic patients at beni-suef, egypt. | amoebiasis diagnosis is usually based on microscopy that cannot differentiate pathogenic e. histolytica from morphologically identical non-pathogenic species. 194 fecal samples were collected from diarrheic &/or dysenteric patients and examined for entamoeba complex microscopically, e. histolytica/e. dispar coproantigen using ict and e. histolytica coproantigen using tech lab e. histolytica ii elisa test. entamoeba complex trophozoites/cysts, e. histolytica/e. dispar coproantigen and e. histolyt ... | 2015 | 25542044 |
| entamoeba infections in different populations of dogs in an endemic area of lahore, pakistan. | entamoeba histolytica, a protozoan parasite that affects humans and other primates all over the world. it is a common waterborne pathogen in endemic areas that have fecal oral transmission cycle. the aim of the present study was to examine the prevalence of e. histolytica and other entamoeba species cysts in three different dog populations. fecal samples from 600 dogs were collected and processed to detect entamoeba cysts using the triple fecal test (light microscopy) and fecal antigens of e. hi ... | 2015 | 25557213 |
| triage and protocol recommendations for the parasitology laboratory based on an epidemiological investigation of parasite diagnostics in ontario laboratories. | a study was performed using a subset of ontario laboratory parasitology data, with three objectives: to describe parasitic infections in ontario; to identify risk factors for acquiring a parasitic infection using routinely collected information; and to use this information to assess current protocols for parasite testing in laboratories and, in turn, to propose alternatives to optimize the allocation of laboratory resources. | 2017 | 25587292 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| notes from the field: outbreak of diarrheal illness caused by shigella flexneri - american samoa, may-june 2014. | on may 9, 2014, a physician at hospital a in american samoa noticed an abnormally high number of children presenting to the emergency department with bloody diarrhea. based on preliminary testing of stool specimens, entamoeba histolytica infection was suspected as a possible cause. shigella was also suspected in a subset of samples. on may 22, the american samoa department of health requested assistance from cdc with the outbreak investigation. the goals of the investigation were to establish th ... | 2015 | 25590684 |
| prevalence of hiv infection and risky sexual practices among adult males with travel-associated entamoeba histolytica infection in taiwan, 2006-2013. | 2015 | 25593040 | |
| prevalence of intestinal parasites and its impact on nutritional status among preschool children living in damanhur city, el-behera governorate, egypt. | this cross sectional study was done in damanhur city, the capital of el-behera governorate to detect the prevalence of intestinal parasitic infection among preschool aged children and to find out its effect on their nutritional status. five-hundreds children aged between 2-6 years were enrolled; a detailed questionnaire, complete clinical and anthropometric assessment as well as complete stool analysis and blood picture were done. the study revealed that 51.8% preschool children were infected; c ... | 2014 | 25597166 |
| is the evaluation of entamoeba histolytica infection in hiv-positive patients of any clinical significance? | amoebiasis caused by entamoeba histolytica (e. histolytica) is one of the most problematic parasitic infections worldwide. data regarding the effect of hiv-induced immunodeficiency on the status of e. histolytica infection are sparse in iran. this study aimed to assess the seroprevalence of anti-e. histolytica igg among iranian hiv patients. further, it determined whether the advancement of immunodeficiency accompanies an increased risk of amoebiasis. a total of 91 hiv-infected patients and 91 c ... | 2015 | 25597605 |
| the role of bifidobacterium lactis b94 plus inulin in the treatment of acute infectious diarrhea in children. | in contrast to many other studies of probiotic species, the number of publications evaluating bifidobacterium lactis and its combinations with prebiotics as treatments for acute infectious diarrhea is limited. we investigated the synbiotic effects of b. lactis b94 plus inulin on acute infectious diarrhea. | 2014 | 25599772 |
| parasitic infection in a young man presenting with non-specific abdominal pain. | a 27-year-old man presented with a two-week history of central colicky abdominal pain associated with loose stools. further history revealed that he had been exposed to contaminated waters. stool investigation by direct wet stool smears revealed the presence of entamoeba histolytica and blastocystis hominis cysts. a diagnosis of amoebiasis secondary to e. histolytica and concurrent b. hominis infestation was made. we would like to emphasise the importance of clinical history including recent tra ... | 2010 | 25606208 |
| maintenance of intracellular hypoxia and adequate heat shock response are essential requirements for pathogenicity and virulence of entamoeba histolytica. | adhesion to cells, cytotoxicity and proteolysis are functions required for virulence and pathogenicity of entamoeba histolytica. however, there was no correlation between these in vitro functions and the early elimination of non-pathogenic e. dispar and non-virulent e. histolytica (nveh) in experimental amoebic liver abscesses developed in hamsters. thus, additional functions may be involved in amoebic pathogenicity and virulence. in the present study, an integral experimental assessment, includ ... | 2015 | 25611463 |
| comparison of four rapid diagnostic tests, elisa, microscopy and pcr for the detection of giardia lamblia, cryptosporidium spp. and entamoeba histolytica in feces. | microscopy is the diagnostic reference standard for the detection of parasites, but it is labor-intensive and requires experience. rapid diagnostic tests (rdts) can provide an alternative to microscopy. | 2015 | 25615719 |
| diversity and prevalence of gastrointestinal parasites in seven non-human primates of the taï national park, côte d'ivoire. | parasites and infectious diseases are well-known threats to primate populations. the main objective of this study was to provide baseline data on fecal parasites in the cercopithecid monkeys inhabiting côte d'ivoire's taï national park. seven of eight cercopithecid species present in the park were sampled: cercopithecus diana, cercopithecus campbelli, cercopithecus petaurista, procolobus badius, procolobus verus, colobus polykomos, and cercocebus atys. we collected 3142 monkey stool samples betw ... | 2015 | 25619957 |
| entamoeba histolytica: adhesins and lectins in the trophozoite surface. | entamoeba histolytica is the causative agent of amebiasis in humans and is responsible for 100,000 deaths annually, making it the third leading cause of death due to a protozoan parasite. pathogenesis appears to result from the potent cytotoxic activity of the parasite, which kills host cells within minutes. although the mechanism is unknown, it is well established to be contact-dependent. the life cycle of the parasite alternates with two forms: the resistant cyst and the invasive trophozoite. ... | 2015 | 25671365 |
| importance of microscopic stool examination in patients with diarrhoea. | loose motion is a common symptom in patients reporting to our hospital. as it is a small set up where only facility for microscopic stool examination is available, we designed this study to know how much microscopic stool examination can help us in management of patients with diarrhoea. | 2015 | 25672169 |
| a pcr-rflp method for the simultaneous differentiation of three entamoeba species. | amoebiasis caused by entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. the aim of this study was to develop a new diagnostic tool to differentiate e.histolytica from the morphologically undistinguishable e.dispar and e.moshkovskii. we developed a specific, fast and simple pcr-rflp method th ... | 2015 | 25680279 |
| molecular and functional characterisation of a stress responsive cysteine protease, ehcp6 from entamoeba histolytica. | entamoeba histolytica cysteine protease 6 (ehcp6) is a stress responsive cysteine protease that is upregulated in response to heat shock and during pathogen invasion of the host tissue. in the present study an attempt has been made to express and purify recombinant ehcp6 in order to gain insights into its biochemical properties. the recombinant and refolded protein has been shown to undergo autoproteolysis in the presence of dtt and sds to give rise to ∼25kda mature form. the mature form of the ... | 2015 | 25680771 |
| a new anthraquinone from morinda elliptica ridl. | a new anthraquinone, morinquinone, together with 18 known anthraquinones were isolated from the stems of morinda elliptica ridl. their structures were elucidated on the basis of spectroscopic data. they each showed weak inhibitory activity against a susceptible strain of staphylococcus aureus and a methicillin-resistant s. aureus. damnacanthal was effective against microsporum gypseum (mic 1 μg/ml). lucidin was active against entamoeba histolytica (mic 31.25 μg/ml) and giardia intestinalis (mic ... | 2015 | 25686628 |
| bioconjugated fluorescent silica nanoparticles for the rapid detection of entamoeba histolytica. | rapid detection of entamoeba histolytica based on fluorescent silica nanoparticle (fsnp) indirect immunofluorescence microscopy was evaluated. silica nanoparticles were synthesized using stöber's method, with their surface activated to covalently bind to, and immobilize, protein a. for biolabeling, fsnp was added to conjugated e. histolytica trophozoites with monoclonal anti-e. histolytica igg1 for microscopic observation of fluorescence. fluorescent silica nanoparticle sensitivity was determine ... | 2015 | 25697865 |
| intestinal parasitic infection among egyptian children with chronic liver diseases. | patients with chronic liver diseases (cld) are often highly susceptible to parasitic infection due to a depressed immune system. the objective of this study was to detect the most commonly intestinal parasites found among egyptian children with cld. the present study was conducted on 50 children with cld of different etiology (25 were having different intestinal symptoms, 25 without intestinal symptoms) and 50 non-cld children with gastrointestinal complaints served as controls. all cases were s ... | 2015 | 25698851 |
| cellular cytokine and chemokine responses to parasite antigens and fungus and mite allergens in children co-infected with helminthes and protozoa parasites. | in sub-saharan africa poly-parasite infections are frequently observed in children, and with poly-parasitism modulating immune mechanisms, mediated by cytokines and chemokines, are required to prevent overwhelming inflammation and host tissue damage. we analyzed in children co-infected with helminthes and protozoan parasites their cellular production of regulatory and pro-inflammatory cytokines and chemokines in response to parasite antigens and allergens. | 2015 | 25698903 |
| molecular and biochemical characterization of entamoeba histolytica fructokinase. | entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess. the medium for its axenic culture contains glucose as energy source, and we addressed the question whether e. histolytica can also use fructose instead. as the amoebic hexokinases do not phosphorylate fructose, a separate fructokinase is essential. the genome project revealed a single candidate gene encoding an e. histolytica homolog of bacterial fructokinases. this gene was cloned, and the recombinant enzyme ha ... | 2015 | 25700717 |
| prevalence of intestinal parasites among the population of the gaza strip, palestine. | intestinal parasitic diseases in gaza strip are a significant health problem. the main aim of this study is to determine the prevalence of parasitic infection among patients in the five governorates of the gaza strip. a cross-sectional parasitological survey was conducted on 600 patients. stool specimens were examined microscopically for the presence of parasite eggs or larvae/cysts per wet amount by the saline iodine and formol-ether concentration method. of 600 subjects examined, 245 (40.8%) w ... | 2014 | 25706427 |
| entamoeba dispar: could it be pathogenic. | amebiasis is a disease caused by the protozoan parasite entamoeba histolytica. this ameba can colonize the human intestine and persist as a commensal parasite, similar to entamoeba dispar, an ameba considered to be non-pathogenic. the similarities between e. histolytica and e. dispar make the latter an attractive model for studies aimed at clarifying the pathogenesis of amebiasis. however, in addition to being an interesting experimental model, this relative of e. histolytica remains poorly unde ... | 2017 | 25709947 |
| a novel mitosomal β-barrel outer membrane protein in entamoeba. | entamoeba possesses a highly divergent mitochondrion-related organelle known as the mitosome. here, we report the discovery of a novel protein in entamoeba, which we name mitosomal β-barrel outer membrane protein of 30 kda (mbomp30). initially identified through in silico analysis, we experimentally confirmed that mbomp30 is indeed a β-barrel protein. circular dichroism analysis showed mbomp30 has a predominant β-sheet structure. localization to entamoeba histolytica mitosomes was observed throu ... | 2015 | 25711150 |
| dramatic decrease in prevalence of soil-transmitted helminths and new insights into intestinal protozoa in children living in the chaco region, bolivia. | we assessed the prevalence of intestinal parasites among 268 2-12-year-old children living in rural areas, small villages, and semi-urban areas of the chaco region, south-eastern bolivia. the overall parasitism was 69%. only protozoa, helminths, or co-infections were observed in 89.2%, 5.9%, or 4.9% of the positive children, respectively. a significant progressive increase in overall parasite prevalence was found when passing from rural areas to small villages and semi-urban areas. the most comm ... | 2015 | 25711609 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| new enzymatic pathways for the reduction of reactive oxygen species in entamoeba histolytica. | entamoeba histolytica, an intestinal parasite that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen and nitrogen species during tissue invasion. a flavodiiron protein and a rubrerythrin have been characterized in this human pathogen, although their physiological reductants have not been identified. | 2015 | 25725270 |
| small gtpase rab21 mediates fibronectin induced actin reorganization in entamoeba histolytica: implications in pathogen invasion. | the protozoan parasite entamoeba histolytica causes a wide spectrum of intestinal infections. in severe cases, the trophozoites can breach the mucosal barrier, invade the intestinal epithelium and travel via the portal circulation to the liver, where they cause hepatic abscesses, which can prove fatal if left untreated. the host extra cellular matrix (ecm) plays a crucial role in amoebic invasion by triggering an array of cellular responses in the parasite, including induction of actin rich adhe ... | 2015 | 25730114 |
| a rare case of multilocus brain abscess due to entamoeba histolytica infection in a child. | brain abscess due to entamoeba histolytica (e. histolytica) may pose a diagnostic problem or a therapeutic challenge, as evidenced by the paucity of papers reporting complete recovery after treatment. an 11-year-old girl presented with progressive drowsiness, diminished movements of the left upper limb, and swallowing problems. cranial mri showed multiple, contrast-dense masses with fluid content. she was started on meropenem. surgical drainage was performed. no bacterial or fungal growth was ob ... | 2015 | 25737180 |
| prevalence of enteric pathogen-associated community gastroenteritis among kindergarten children in gaza. | gastroenteritis is considered as one of the leading causes of illness and death in children under 5 years age, especially in developing countries. it is one of the major public health problems among childhood in gaza strip, palestine. this study was conducted to determine the prevalence of enteric pathogen-associated community gastroenteritis among kindergarten children in gaza. a total of 150 stool samples were collected and investigated for parasitic, viral and bacterial pathogens at al azhar ... | 2014 | 25745477 |
| enteric pathogens of food sellers in rural gambia with incidental finding of myxobolus species (protozoa: myxozoa). | ongoing surveillance of enteric pathogens of public health significance among casual food sellers is undertaken in many resource-limited countries. we report the results of a survey in kiang west province, the gambia, and provide an exemplar methodology for such surveys in resource-limited laboratories. | 2015 | 25758855 |
| application of multiplex pcr for detection and differentiation of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii. | entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica. multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. | 2017 | 25759727 |
| functional correlation of medicinal chemistry and entamoeba histolytica treatment: an emphasis on the past, present and future chemotherapy. | entamoeba being an internal parasite is the causative organism of many human and animal infections with lethal consequences. chemotherapy against entamoeba was well established by both synthetic and natural products. vigorous attainments were made by different researchers all over the world for discovering better chemical entities and the potent formulations. preexisting compounds were developed into non-cytotoxic and with an enhanced therapeutic compounds by usage of multiparametric optimizatio ... | 2015 | 25769970 |
| molecular detection of entamoeba histolytica and entamoeba dispar infection among wild rats in kuala lumpur, malaysia. | entamoeba histolytica infection is the third-greatest parasitic disease responsible for death in the world. wild rats harbouring e. histolytica can be the possible reservoir hosts for human amoebiasis. there were numerous studies on prevalence of intestinal parasites among wild rats in malaysia but none has reported e. histolytica. rats were captured from sentul and chow kit areas, kuala lumpur, malaysia. the preserved stool samples were used for microscopy examination and molecular analysis. ou ... | 2014 | 25776597 |
| homology modeling of mdr1 gene mdr1_enthi of e. histolytica & its molecular docking with anti-entamoeba histolytica agents. | to investigate the theoretical model of the three-dimensional structure of multidrug resistance protein 1(p-glycoprotein, expressed by mdr1 gene of e. histolytica) and its molecular docking with anti-amoebic agents. the theoretical model of multidrug resistance protein 1(p-glyoprotein, expressed by mdr1 gene of e. histolytica) was predicted by homology modeling on the structure of the 3g61. docking studies were performed to investigate the interaction of p-glycoprotein with antiamoebic gents. mr ... | 2015 | 25786505 |
| pyrazolo[3,4-d]pyrimidines as novel inhibitors of o-acetyl-l-serine sulfhydrylase of entamoeba histolytica: an in silico study. | amoebiasis, a worldwide explosive epidemic, caused by the gastrointestinal anaerobic protozoan parasite entamoeba histolytica, infects the large intestine and, in advance stages, liver, kidney, brain and lung. metronidazole (mnz)-the first line medicament against amoebiasis-is potentially carcinogenic to humans and shows significant side-effects. pyrazolo[3,4-d]pyrimidine compounds have been reported to demonstrate antiamoebic activity. in silico molecular docking simulations on nine pyrazolo[3, ... | 2015 | 25799964 |
| correction: crystal structure of calcium binding protein-5 from entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes. | 2015 | 25816121 | |
| roles and regulation of the mucus barrier in the gut. | the gastrointestinal tract is coated by a thick layer of mucus that forms the front line of innate host defense. mucus consists of high molecular weight glycoproteins called mucins that are synthesized and secreted by goblet cells and functions primarily to lubricate the epithelium and protect it from damage by noxious substances. recent studies have also suggested the involvement of goblet cells and mucins in complex immune functions such as antigen presentation and tolerance. under normal phys ... | 2015 | 25838985 |
| antiprotozoal nitazoxanide derivatives: synthesis, bioassays and qsar study combined with docking for mechanistic insight. | in view of the serious health problems concerning infectious diseases in heavily populated areas, we followed the strategy of lead compound diversification to evaluate the near-by chemical space for new organic compounds. to this end, twenty derivatives of nitazoxanide (ntz) were synthesized and tested for activity against entamoeba histolytica parasites. to ensure drug-likeliness and activity relatedness of the new compounds, the synthetic work was assisted by a quantitative structure-activity ... | 2015 | 25872791 |
| maldi-tof mass spectrometry for the detection and differentiation of entamoeba histolytica and entamoeba dispar. | detection of entamoeba histolytica and its differentiation from entamoeba dispar is an important goal of the clinical parasitology laboratory. the aim of this study was the identification and differentiation of e. histolytica and e. dispar by maldi-tof ms, in order to evaluate the application of this technique in routine diagnostic practice. maldi-tof ms was applied to 3 amebic reference strains and to 14 strains isolated from feces that had been differentiated by molecular methods in our labora ... | 2015 | 25874612 |
| assessments of immunomodulatory and inflammatory effects against induction of entamoeba histolytica (hm1 ims strain) crude extract antigen in complete freund's adjuvant induced rheumatoid arthritis female wistar rats. | today it is well known about mechanisms of cell communication, how the cells that mediate immune response and tissue injury accumulate in tissues but the aetiology of rheumatoid arthritis (ra) is still unknown. this study was to evaluate immunomodulatory effects of crude entamoeba histolytica (hm1 ims strain) antigen in complete freund's adjuvant female wistar rats by studying the alterations in humoral and cell mediated immune responses and also the inflammatory effects by evaluating the change ... | 2015 | 25881902 |
| seroepidemiology of entamoeba histolytica infection in general population in rural durango, mexico. | the seroepidemiology of entamoeba histolytica in mexico has been scantily studied. the aim of the study was to determine the seroprevalence and correlates of e. histolytica antibodies in adults in rural areas in durango, mexico. | 2015 | 25883706 |
| chemokine levels and parasite- and allergen-specific antibody responses in children and adults with severe or uncomplicated plasmodium falciparum malaria. | chemokine and antibody response profiles were investigated in children and adults with severe or uncomplicated plasmodium falciparum malaria; the aim was to reveal which profiles are associated with severe disease, as often seen in nonimmune children, or with mild and uncomplicated disease, as seen in semi-immune adults. blood samples were obtained from children under 5 years of age as well as adults with falciparum malaria. classification of malaria was performed according to parasite densities ... | 2015 | 25883801 |
| entamoeba histolytica rhomboid protease 1 has a role in migration and motility as validated by two independent genetic approaches. | rhomboid proteins represent a recently discovered family of intramembrane proteases present in a broad range of organisms and with increasing links to human diseases. the enteric parasite entamoeba histolytica has evolved multiple mechanisms to adapt to the human host environment and establish infection. our recent studies identified ehrom1 as a functional e. histolytica rhomboid protease with roles in adhesion to and phagocytosis of host cells. since those studies were performed in a non-virule ... | 2015 | 25889553 |
| entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the h4 histone. | in eukaryotes, histone arginine methylation associates with both active and repressed chromatin states depending on the residues involved and the status of methylation. even when the amino-terminus of entamoeba histolytica histones diverge from metazoan sequences, these regions contain arginine residues that are potential targets for methylation. however, histone arginine methylation as well as the activity of arginine methyltransferases (prmts) has not been studied in this parasite. the aim of ... | 2015 | 25889855 |
| [analysis on parasitic infection of clinical samples from hospitals in shanghai during 2011-2013]. | to analyze the results of parasitic pathogen detection on clinical samples from shanghai hospitals during 2011-2013. | 2014 | 25902676 |
| identification of entamoeba histolytica by molecular method in surface water of rasht city, iran. | this study was conducted with the aim of determining surface water contamination with cysts of entamoeba histolytica using pcr in rasht city, northern iran. | 2015 | 25905058 |
| spectrum of enteropathogens detected by the filmarray gi panel in a multicentre study of community-acquired gastroenteritis. | the european, multicentre, quarterly point-prevalence study of community-acquired diarrhoea (eucodi) analysed stool samples received at ten participating clinical microbiology laboratories (austria, finland, france, germany, greece, ireland, italy, portugal, romania, and the uk) in 2014. on four specified days, each local laboratory submitted samples from ≤20 consecutive patients to the austrian study centre for further testing with the filmarray gi panel (biofire diagnostics, salt lake city, ut ... | 2015 | 25908431 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| [amoebic liver abscess in a patient initially diagnosed with pneumonia: case report and discussion of relevant literature]. | in one-third of the patients with amoebiasis, amoebic liver abscess (ala) may occur after the penetration of amoebic trophozoites through the intestinal wall. ala is seen mostly among men aged 20-45 years with a serious clinical outcome, with fever and abdominal pain on the right upper quadrant. most patients have no recent history of amoebic colitis; indeed, they have neither gastrointestinal complaints nor entamoeba histolytica (e. histolytica) cysts/trophozoites in their stools. therefore, ul ... | 2015 | 25917589 |
| prevalence of intestinal protozoan infections among individuals living with hiv/aids at felegehiwot referral hospital, bahir dar, ethiopia. | hiv infection continues to pose a serious challenge to global health by predisposing patients to opportunistic infections. a cross-sectional study was conducted from december 2012 to february 2013 to assess the enteric protozoan infection status among individuals living with hiv/aids in felegehiwot referral hospital, bahir dar, northwest ethiopia. | 2015 | 25917962 |
| prevalence of intestinal parasites among food handlers of sari, northern iran. | parasitic infection is highly prevalent throughout the developing countries of the world. food handlers are a potential source of infection for many intestinal parasites and other enteropathogenic infections as well. the aim of this study was to determine the prevalence of intestinal parasite carriers among food handlers attending the public health center laboratory in sari, northern iran for annual check-up. the study was performed from august 2011 through february 2012. stool samples were coll ... | 2017 | 25923893 |