Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| desideratum: a developmentalist view of zika virus infection. | 2020 | 33068527 | |
| vertical transmission of zika virus and its outcomes: a bayesian synthesis of prospective studies. | prospective studies of zika virus in pregnancy have reported rates of congenital zika syndrome and other adverse outcomes by trimester. however, zika virus can infect and damage the fetus early in utero, but clear before delivery. the true vertical transmission rate is therefore unknown. we aimed to provide the first estimates of underlying vertical transmission rates and adverse outcomes due to congenital infection with zika virus by trimester of exposure. | 2020 | 33068528 |
| curcumin allosterically inhibits the dengue ns2b-ns3 protease by disrupting its active conformation. | flaviviruses including dengue virus and zika virus encode a unique two-component ns2b-ns3 protease essential for maturation/infectivity, thus representing a key target for designing antiflavivirus drugs. here, for the first time, by nmr and molecular docking, we reveal that curcumin allosterically inhibits the dengue protease by binding to a cavity with no overlap with the active site. further molecular dynamics simulations decode that the binding of curcumin leads to unfolding/displacing the ch ... | 2020 | 33073093 |
| zika virus depletes neural stem cells and evades selective autophagy by suppressing the fanconi anemia protein fancc. | zika virus (zikv) is an emerging flavivirus, which when passed through vertical transmission from mother to developing fetus can lead to developmental abnormalities, including microcephaly. while there is mounting evidence that suggests a causal relationship between zikv infection and microcephaly, the mechanisms by which zikv induces these changes remain to be elucidated. here, we demonstrate that zikv infection of neural stems cells, both in vitro and in vivo, induces macroautophagy to enhance ... | 2020 | 33073500 |
| development and optimization of a zika virus antibody-dependent cell-mediated cytotoxicity (adcc) assay. | zika virus (zikv) has become a global public health issue due to its teratogenicity and ability to cause guillain-barré syndrome in adults. although anti-zikv envelope protein neutralizing antibodies correlate with protection, the non-neutralizing function of zikv antibodies including antibody-dependent cell-mediated cytotoxicity (adcc) is incompletely understood. to study the role of adcc antibodies during zikv infections, we generated a stably transfected, dual-reporter target cell line with i ... | 2020 | 33075363 |
| defa1b inhibits zikv replication and retards cell cycle progression through interaction with orc1. | zika virus (zikv) infection causes a public health concern because of its potential association with the development of microcephaly. during viral infections, the host innate immune response is mounted quickly to produce some endogenous functional molecules to limit virus replication and spread. exosomes contain molecules from their cell of origin following virus infection and can enter recipient cells for intercellular communication. here, we aim to clarify whether zikv-induced exosomes can reg ... | 2020 | 33075374 |
| generation and preliminary characterization of vertebrate-specific replication-defective zika virus. | zika virus (zikv) is a mosquito-borne flavivirus that replicates in both vertebrate and insect cells, whereas insect-specific flaviviruses (isf) replicate only in insect cells. we sought to convert zikv, from a dual-tropic flavivirus, into an insect-specific virus for the eventual development of a safe zikv vaccine. reverse genetics was used to introduce specific mutations into the furin cleavage motif within the zikv pre-membrane protein (prm). mutant clones were selected, which replicated well ... | 2020 | 33075709 |
| antibodies targeting epitopes on the cell-surface form of ns1 protect against zika virus infection during pregnancy. | there are no licensed therapeutics or vaccines available against zika virus (zikv) to counteract its potential for congenital disease. antibody-based countermeasures targeting the zikv envelope protein have been hampered by concerns for cross-reactive responses that induce antibody-dependent enhancement (ade) of heterologous flavivirus infection. nonstructural protein 1 (ns1) is a membrane-associated and secreted glycoprotein that functions in flavivirus replication and immune evasion but is abs ... | 2020 | 33077712 |
| methylene blue is a potent and broad-spectrum inhibitor against zika virus in vitro and in vivo. | many flaviviruses including the dengue virus (denv), zika virus (zikv), west nile virus, yellow fever virus, and japanese encephalitis virus are significant human pathogens, unfortunately without any specific therapy. here, we demonstrate that methylene blue, an fda-approved drug, is a broad-spectrum and potent antiviral against zika virus and dengue virus both in vitro and in vivo. we found that methylene blue can considerably inhibit the interactions between viral protease ns3 and its ns2b co- ... | 2020 | 33078696 |
| preventing vector-borne transmission of zika virus infection during pregnancy, puerto rico, usa, 2016-20171. | we examined pregnant women's use of personal protective measures to prevent mosquito bites during the 2016-2017 zika outbreak in puerto rico. healthcare provider counseling on recommended measures was associated with increased use of insect repellent among pregnant women but not with wearing protective clothing. | 2020 | 33079044 |
| zika virus ns4a n-terminal region (1-48) acts as a cofactor for inducing ntpase activity of ns3 helicase but not ns3 protease. | among flaviviridae, in west nile virus (wnv) and hepatitis c virus (hcv), the non-structural protein ns4a modulates the ntpase activity of viral helicases during nucleic acid unwinding through its n-terminal disordered residues (1-50). in hcv, the acidic ns4a also serves as a cofactor for regulating the ns3 protease activity. however, in case of zika virus (zikv), the role of ns4a and its impact on activities of ns3 helicase and protease is not known. in order to elucidate the role of ns4a, we c ... | 2020 | 33080173 |
| betulinic acid exhibits antiviral effects against dengue virus infection. | dengue virus (denv) is an arthropod-borne virus that has developed into a prominent global health threat in recent decades. the main causative agent of dengue fever, the virus infects an estimated 390 million individuals across the globe each year. despite the sharply increasing social and economic burden on global society caused by the disease, there is still a glaring lack of effective therapeutics against denv. in this study, betulinic acid, a naturally occurring pentacyclic triterpenoid was ... | 2020 | 33080251 |
| accuracy of the zika igm antibody capture enzyme-linked immunosorbent assay from the centers for disease control and prevention (cdc zika mac-elisa) for diagnosis of zika virus infection. | serological diagnosis of zika virus (zikv) infection is challenging because of antigenic cross-reactivity with dengue virus (denv). this study evaluated the accuracy of the zika igm antibody capture enzyme-linked immunosorbent assay (cdc zika igm mac-elisa) in differentiating between zikv and denv infections. to determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with rt-pcr-confirmed zikv infection. to determine specificity, we used acute- and convalescent-phase s ... | 2020 | 33080935 |
| disruption of zika virus xrrna1-dependent sfrna1 production results in tissue-specific attenuated viral replication. | the zika virus (zikv), like other flaviviruses, produces several species of sub-genomic rnas (sfrnas) during infection, corresponding to noncoding rna fragments of different lengths that result from the exonuclease degradation of the viral 3' untranslated region (utr). over the course of infection, these sfrnas accumulate in the cell as a result of an incomplete viral genome degradation of the 3' utr by the host 5' to 3' exoribonuclease, xrn1. the halting of xrn1 in the 3' utr is due to two rna ... | 2020 | 33080971 |
| mental models of infectious diseases and public understanding of covid-19 prevention. | the emergence of viral diseases such as ebola virus disease, zika virus disease, and the coronavirus disease (covid-19) has posed considerable challenges to health care systems around the world. public health strategy to address emerging infectious diseases has depended in part on human behavior change and yet the perceptions and knowledge motivating that behavior have been at times inconsistent with the latest consensus of peer-reviewed science. part of that disjuncture likely involves the exis ... | 2020 | 33081500 |
| prenatal cytomegalovirus, rubella, and zika virus infections associated with developmental disabilities: past, present, and future. | prenatal infections have long been recognized as important, preventable causes of developmental disabilities. the list of pathogens that are recognized to have deleterious effects on fetal brain development continues to grow, most recently with the association between zika virus (zikv) and microcephaly. to answer clinical questions in real time about the impact of a novel infection on developmental disabilities, an historical framework is key. the lessons learned from three historically importan ... | 2020 | 33084055 |
| what are the implications of zika virus for infant feeding? a synthesis of qualitative evidence concerning congenital zika syndrome (czs) and comparable conditions. | if a mother contracts the zika virus before or during pregnancy, then there is a risk of the child developing congenital zika syndrome (czs). an infant can then experience problems feeding due to the specific physical and developmental consequences of congenital zika syndrome (czs), such as microcephaly, dysphagia and an increased likelihood of choking. this qualitative evidence synthesis accesses direct and indirect evidence to inform who infant feeding guidelines. we conducted a qualitative ev ... | 2020 | 33085668 |
| prevalence and diagnostic accuracy of microcephaly in a pediatric cohort in brazil: a retrospective cross-sectional study. | we sought to describe the prevalence of microcephaly and to compare the different cutoff points established by the brazilian ministry of health (moh) at various times during a zika virus epidemic. as a secondary aim, we investigated the possible etiology of the microcephaly. | 2020 | 33086050 |
| association between brain morphology and electrophysiological features in congenital zika virus syndrome: a cross-sectional, observational study. | intrauterine infection with the zika virus (zikv) has been connected to severe brain malformations, microcephaly, and abnormal electrophysiological activity. | 2020 | 33089122 |
| animal models of congenital zika syndrome provide mechanistic insight into viral pathogenesis during pregnancy. | in utero zika virus (zikv; family flaviviridae) infection causes a distinct pattern of birth defects and disabilities in the developing fetus and neonate that has been termed congenital zika syndrome (czs). over 8,000 children were affected by the 2016 to 2017 zikv outbreak in the americas, many of whom developed czs as a result of in utero exposure. to date, there is no consensus about how zikv causes czs; animal models, however, are providing mechanistic insights. using nonhuman primates, immu ... | 2020 | 33091001 |
| quantitative definition of neurobehavior, vision, hearing and brain volumes in macaques congenitally exposed to zika virus. | congenital zika virus (zikv) exposure results in a spectrum of disease ranging from severe birth defects to delayed onset neurodevelopmental deficits. zikv-related neuropathogenesis, predictors of birth defects, and neurodevelopmental deficits are not well defined in people. here we assess the methodological and statistical feasibility of a congenital zikv exposure macaque model for identifying infant neurobehavior and brain abnormalities that may underlie neurodevelopmental deficits. we inocula ... | 2020 | 33091010 |
| novel nucleoside analogues as effective antiviral agents for zika virus infections. | previously considered a neglected flavivirus, zika virus has recently emerged as a public health concern due to its ability to spread rapidly and cause severe neurological disorders, such as microcephaly in newborn babies from infected mothers, and guillain-barré syndrome in adults. despite extensive efforts towards the identification of effective therapies, specific antivirals are still not available. as part of ongoing medicinal chemistry studies to identify new antiviral agents, we screened a ... | 2020 | 33092055 |
| utility of proteomics in emerging and re-emerging infectious diseases caused by rna viruses. | emerging and re-emerging infectious diseases due to rna viruses cause major negative consequences for the quality of life, public health, and overall economic development. most of the rna viruses causing illnesses in humans are of zoonotic origin. zoonotic viruses can directly be transferred from animals to humans through adaptation, followed by human-to-human transmission, such as in human immunodeficiency virus (hiv), severe acute respiratory syndrome coronavirus (sars-cov), middle east respir ... | 2020 | 33095583 |
| high seroprevalence of antibodies against arboviruses among pregnant women in rural caribbean colombia in the context of the zika virus epidemic. | mosquito-borne viruses such as dengue (denv), chikungunya (chikv), and zika (zikv) have spread in recent decades. we aimed to assess seroprevalence of arboviral infections in pregnant women living in cereté, caribbean colombia. in 2016 a cross-sectional facility-based sero-survey study was performed among pregnant women (n = 90). most of them (66%) reported at least one symptom or sign compatible with arboviral infection over the previous 15 days. all screened women had a positive igg for denv, ... | 2020 | 33096597 |
| extracellular vesicles in the pathogenesis of viral infections in humans. | most cells can release extracellular vesicles (evs), membrane vesicles containing various proteins, nucleic acids, enzymes, and signaling molecules. the exchange of evs between cells facilitates intercellular communication, amplification of cellular responses, immune response modulation, and perhaps alterations in viral pathogenicity. evs serve a dual role in inhibiting or enhancing viral infection and pathogenesis. this review examines the current literature on evs to explore the complex role o ... | 2020 | 33096825 |
| six cases of zika/dengue coinfection in a brazilian cohort, 2015-2019. | brazil is one of the countries which has been most affected by dengue epidemics. this scenario became more challenging with the emergence of zika virus after 2014. the cocirculation of dengue and zika viruses makes their diagnosis and treatment a challenge for health professionals, especially due to their similar clinical outcomes. from 2015 to 2019, we followed a cohort of 2017 participants in goiania, goias, central brazil. febrile cases were monitored weekly, and after identification of fever ... | 2020 | 33096849 |
| comparison of zika virus inactivation methods for reagent production and disinfection methods. | zika virus (zikv) infection remains a public health concern necessitating demand for long-term virus production for diagnostic assays and r&d activities. inactivated virus constitutes an important component of the trioplex rrt-pcr assay and serological igm assay (mac-elisa). the aim of our study is to establish standard methods of zikv inactivation while maintaining antigenicity and rna integrity. we tested viral supernatants by four different inactivation methods: 1. heat inactivation at 56 °c ... | 2020 | 33098957 |
| detection and discrimination of multiple strains of zika virus by reverse transcription-loop-mediated isothermal amplification. | monitoring both invasion of zika virus disease into free countries and circulation in endemic countries is essential to avoid a global pandemic. however, the difficulty lies in detecting zika virus due to the large variety of mutations in its genomic sequence. to develop a rapid and simple method with high accuracy, reverse transcription-loop-mediated isothermal amplification (rt-lamp) was adopted for the detection of zika virus strains derived from several countries. | 2020 | 33100882 |
| japanese encephalitis virus vaccination elicits cross-reactive hla-class i-restricted cd8 t cell response against zika virus infection. | japanese encephalitis virus (jev) exposure or vaccination could elicit cross-reactive cd8 t cell immunity against heterologous flaviviruses in humans. in addition, cross-reactive cd8 t cells induced by dengue virus (denv) have been shown to play a protective role against zika virus (zikv). however, how jev exposure or vaccination affects zikv infection in humans remains unclear. in this report, epitope prediction algorithms were used to predict the cross-reactive cd8 t cell epitope restricted to ... | 2020 | 33101303 |
| from risk butterflies to citizens engaged in risk prevention in the zika virus crisis: focusing on personal, societal and global risk perceptions. | this study investigates how posting information about the zika virus on social media, and receiving information about zika through the same platforms, are associated with personal, societal and global risk perceptions, and whether different types of risk perceptions are related to information seeking and preventive behavior intentions. a survey of u.s. participants (n = 958) revealed that posting information on social media is positively associated with personal and societal risk perceptions but ... | 2020 | 33103609 |
| the vertical stratification of potential bridge vectors of mosquito-borne viruses in a central amazonian forest bordering manaus, brazil. | the emergence of zika virus (zikv) in latin america brought to the fore longstanding concerns that forests bordering urban areas may provide a gateway for arbovirus spillback from humans to wildlife. to bridge urban and sylvatic transmission cycles, mosquitoes must co-occur with both humans and potential wildlife hosts, such as monkeys, in space and time. we deployed bg-sentinel traps at heights of 0, 5, 10, and 15 m in trees in a rainforest reserve bordering manaus, brazil, to characterize the ... | 2020 | 33106507 |
| zika virus syndrome, lack of environmental policies and risks of worsening by cyanobacteria proliferation in a climate change scenario. | almost half of the brazilian population has no access to sewage collection and treatment. untreated effluents discharged in waters of reservoirs for human supply favor the flowering of cyanobacteria - and these microorganisms produce toxins, such as saxitoxin, which is a very potent neurotoxin present in reservoirs in the northeast region. a recent study confirmed that chronic ingestion of neurotoxin-infected water associated with zika virus infection could lead to a microcephaly-like outcome in ... | 2020 | 33111925 |
| new antibacterial peptides from the freshwater mollusk pomacea poeyana (pilsbry, 1927). | antimicrobial peptides (amps) are biomolecules with antimicrobial activity against a broad group of pathogens. in the past few decades, amps have represented an important alternative for the treatment of infectious diseases. their isolation from natural sources has been widely investigated. in this sense, mollusks are promising organisms for the identification of amps given that their immune system mainly relies on innate response. in this report, we characterized the peptide fraction of the cub ... | 2020 | 33113998 |
| hijacking of lipid droplets by hepatitis c, dengue and zika viruses-from viral protein moonlighting to extracellular release. | hijacking and manipulation of host cell biosynthetic pathways by human enveloped viruses are essential for the viral lifecycle. flaviviridae members, including hepatitis c, dengue and zika viruses, extensively manipulate host lipid metabolism, underlining the importance of lipid droplets (lds) in viral infection. lds are dynamic cytoplasmic organelles that can act as sequestration platforms for a unique subset of host and viral proteins. transient recruitment and mobilization of proteins to lds ... | 2020 | 33114346 |
| phosphotidylethanolamine and phosphatidylserine synergize to enhance gas6/axl-mediated virus infection and efferocytosis. | phosphatidylserine (ps) receptors mediate clearance of apoptotic cells-efferocytosis-by recognizing the ps exposed on those cells. they also mediate the entry of enveloped viruses by binding ps in the virion membrane. here we show that phosphatidylethanolamine (pe) synergizes with ps to enhance ps receptor-mediated efferocytosis and virus entry. the presence of pe on the same surface as ps dramatically enhances recognition of ps by ps-binding proteins such as gas6, pros, and tim1. liposomes cont ... | 2020 | 33115868 |
| nonhuman primates exposed to zika virus in utero are not protected against reinfection at 1 year postpartum. | there is limited information about the impact of zika virus (zikv) exposure in utero on the anti-zikv immune responses of offspring. we infected six rhesus macaque dams with zikv early or late in pregnancy and studied four of their offspring over the course of a year postpartum. despite evidence of zikv exposure in utero, we observed no structural brain abnormalities in the offspring. we detected infant-derived zikv-specific immunoglobulin a antibody responses and t cell memory responses during ... | 2020 | 33115950 |
| viral-immune cell interactions at the maternal-fetal interface in human pregnancy. | the human decidua and placenta form a distinct environment distinguished for its promotion of immunotolerance to infiltrating semiallogeneic trophoblast cells to enable successful pregnancy. the maternal-fetal interface also successfully precludes transmission of most pathogens. this barrier function occurs in conjunction with a diverse influx of decidual immune cells including natural killer cells, macrophages and t cells. however, several viruses, among other microorganisms, manage to escape d ... | 2020 | 33117336 |
| zika virus testing of asymptomatic patients undergoing assisted reproduction in curitiba, brazil. | the objective is to report recent data on the infection and detection of zika virus in infertile couples and to discuss the need to make disease surveillance compulsory in this population in order to decrease the burden on the healthcare system and expedite treatment onset. | 2020 | 33118718 |
| a novel synthetic dna vaccine elicits protective immune responses against powassan virus. | powassan virus (powv) infection is a tick-borne emerging infectious disease in the united states and north america. like zika virus, powv is a member of the family flaviviridae. powv causes severe neurological sequalae, meningitis, encephalitis, and can cause death. although the risk of human powv infection is low, its incidence in the u.s. in the past 16 years has increased over 300%, urging immediate attention. despite the disease severity and its growing potential for threatening larger popul ... | 2020 | 33119599 |
| transmission dynamics of zika virus incorporating harvesting. | in this paper, we establish a zikv model and investigate the transmission dynamics of zikv with two types of harvesting: proportional harvesting and constant harvesting, and give the stability of the steady states of both disease-free and endemic equilibrium, analyze the effect of harvesting on zikv transmission dynamics via numerical simulation. we find that proportional harvesting strategy can eliminate the virus when the basic reproduction number $r_0$ is less than 1, but the constant harvest ... | 2020 | 33120594 |
| impact of zika virus infection on human neural stem cell microrna signatures. | zika virus (zikv) is a mosquito-borne virus, which can cause brain abnormalities in newborns, including microcephaly. micrornas (mirnas) are small non-coding rnas, which post- transcriptionally regulate gene expression. they are involved in various processes including neurological development and host responses to viral infection, but their potential role in zikv pathogenesis remains poorly understood. mirnas can be incorporated into extracellular vesicles (evs) and mediate cell-to-cell communic ... | 2020 | 33121145 |
| factors influencing preventive behaviors for zika virus infection in female nursing students: a cross-sectional study. | backgrounds: zika virus infection may cause severe complications in women of childbearing age. female nursing college students, in the role of future nurses and women of childbearing age, can be effective educators and health supporters by stimulating preventive behaviors. objectives: the purpose of this study was to identify the level of knowledge, attitudes, and preventive behaviors for zika virus infection and determine the factors that influence this behavior in female nursing students. meth ... | 2020 | 33121360 |
| zika virus. | zika virus (zikv), a neurotropic single-stranded rna flavivirus, remains an important cause of congenital infection, fetal microcephaly, and guillain-barré syndrome in populations where zikv has adapted to a nexus involving the aedes mosquitoes and humans. to date, outbreaks of zikv have occurred in africa, southeast asia, the pacific islands, the americas, and the caribbean. emerging evidence, however, suggests that the virus also has the potential to cause infections in europe, where autochton ... | 2020 | 33126413 |
| pregnancy alters innate and adaptive immune responses to zika virus infection in the reproductive tract. | recent outbreaks of zika virus (zikv) have been associated with birth defects, including microcephaly and neurologic impairment. however, the mechanisms that confer potential susceptibility to zikv during pregnancy remain unclear. we hypothesized that poor outcomes from zikv infection during pregnancy are due in part to pregnancy-induced alteration of innate immune cell frequencies and cytokine expression. to examine the impact of pregnancy on innate immune responses, we inoculated immunocompete ... | 2020 | 33127823 |
| molecular mechanisms underlying the extreme mechanical anisotropy of the flaviviral exoribonuclease-resistant rnas (xrrnas). | mechanical anisotropy is an essential property for many biomolecules to assume their structures, functions and applications, however, the mechanisms for their direction-dependent mechanical responses remain elusive. herein, by using a single-molecule nanopore sensing technique, we explore the mechanisms of directional mechanical stability of the xrrna1 rna from zika virus (zikv), which forms a complex ring-like architecture. we reveal extreme mechanical anisotropy in zikv xrrna1 which highly dep ... | 2020 | 33127896 |
| no effect of prior dengue virus 1 infection in mouse dams on long-term behavioral profiles in offspring infected with zika virus during gestation. | zika virus (zikv) is a mosquito-borne flavivirus structurally and antigenically related to dengue virus (denv). zika virus has been associated with congenital anomalies and most zikv outbreaks have occurred in endemic areas of denv. the present study investigated the effects of prior denv serotype 1 (denv1) immunity in immunocompetent female swiss mice on gestational zikv infection in offspring. physical/reflex development, locomotor activity, anxiety, visual acuity, and brain-derived neurotroph ... | 2020 | 33129847 |
| impact of zika virus on the human type i interferon osteoimmune response. | the developing field of osteoimmunology supports importance of an interferon (ifn) response pathway in osteoblasts. clarifying osteoblast-ifn interactions is important because ifn is used as salvage anti-tumor therapy but systemic toxicity is high with variable clinical results. in addition, osteoblast response to systemic bursts and disruptions of ifn pathways induced by viral infection may influence bone remodeling. zika virus (zikv) infection impacts bone development in humans and ifn respons ... | 2020 | 33130337 |
| adapting the ages and stages questionnaire to identify and quantify development among children with evidence of zika infection. | this article describes novel methods of applying the ages and stages questionnaire-3rd edition (asq-3) to assess and quantify developmental delay among children following the 2015-2016 zika virus outbreak in brazil. many of the children with zika virus infection were expected to have severe developmental delay. however, administering the asq-3 to caregivers of these children according to standard protocol would have screened for the overall presence of delay but not the severity of delay. we ado ... | 2020 | 33132516 |
| zika virus infection results in biochemical changes associated with rna editing, inflammatory and antiviral responses in aedes albopictus. | rapid and significant range expansion of both the zika virus (zikv) and its aedes vector species has resulted in the declaration of zikv as a global health threat. successful transmission of zikv by its vector requires a complex series of interactions between these entities including the establishment, replication and dissemination of the virus within the mosquito. the metabolic conditions within the mosquito tissues play a critical role in mediating the crucial processes of viral infection and ... | 2020 | 33133033 |
| potent restriction of sexual zika virus infection by the lipid fraction of extracellular vesicles in semen. | sexual zika virus (zikv) transmission from men to women occurs less frequently than the often-detected high viral loads in semen would suggest, but worries that this transmission route predisposes to fetal damage in pregnant women remain. to better understand sexual zikv pathogenesis, we studied the permissiveness of the human female genital tract to infection and the effect of semen on this process. zikv replicates in vaginal tissues and primary epithelial cells from the vagina, ectocervix, and ... | 2020 | 33133043 |
| modelling of pathogen-host systems using deeper orf annotations and transcriptomics to inform proteomics analyses. | the zika virus is a flavivirus that can cause fulminant outbreaks and lead to guillain-barré syndrome, microcephaly and fetal demise. like other flaviviruses, the zika virus is transmitted by mosquitoes and provokes neurological disorders. despite its risk to public health, no antiviral nor vaccine are currently available. in the recent years, several studies have set to identify human host proteins interacting with zika viral proteins to better understand its pathogenicity. yet these studies us ... | 2020 | 33133425 |
| insights of novel coronavirus (sars-cov-2) disease outbreak, management and treatment. | emerging and re-emerging viral diseases poses a threat to living organisms, and led to serious concern to humankind and public health. the last two decades, viral epidemics such as the severe acute respiratory syndrome (sars-cov) reported in the years 2002-2003, and h1n1 influenza (swine flu) in 2009, middle east respiratory syndrome (mers-cov) from saudi arabia in 2012, ebola virus in 2014-2016, and zika virus in 2015. the recent outbreak of 2019-cov-2 or severe acute respiratory syndrome-2 (sa ... | 2020 | 33134740 |
| current status and a perspective of mosquito-borne diseases in the republic of korea. | background: mosquito-borne diseases in the republic of korea have a unique epidemiology due to the rapid improvement in hygiene and economic status, occurrence of four distinct seasons, and separation from north korea owing to the political situation. therefore, we aimed to analyze and review the epidemiology of mosquito-borne diseases in korea. methods: the incidence and geographical distribution of malaria, japanese encephalitis (je), zika virus infection, chikungunya fever, and dengue fever w ... | 2020 | 33136531 |
| characterization of visual pathway abnormalities in infants with congenital zika syndrome using computed tomography and magnetic resonance imaging. | severe visual impairment is present in nearly all infants with congenital zika syndrome (czs); however, ocular abnormalities are present only in a subset of these infants. the purpose of this study was to characterize the visual pathway abnormalities seen on computed tomography (ct) and mri scans in infants with czs. | 2020 | 33136667 |
| role of zika virus infection in cases and outbreaks of guillain-barré syndrome in latin america: pooling the prevalence of studies. | 2020 | 33137492 | |
| zika virus-infected decidual cells elicit a gestational age-dependent innate immune response and exaggerate trophoblast zika permissiveness: implication for vertical transmission. | vertical transmission of the zika virus (zikv) causes severe fetal defects, but the exact pathogenic mechanism is unclear. we identified up to a 10,480-fold higher expression of viral attachment factors axl, gas6, and pros1 and a 3880-fold increase in zikv infectiousness/propagation in human term decidual stromal cells versus trophoblasts. moreover, levels of viral attachment factors and zikv are significantly increased, whereas expression of innate immune response genes are significantly decrea ... | 2020 | 33139490 |
| the role of community engagement in addressing bystander risks in research: the case of a zika virus controlled human infection study. | there is limited guidance on how to assess the ethical acceptability of research risks that extend beyond research participants to third parties (or "research bystanders"). community or stakeholder engagement has been proposed as one way to address potential harms to community members, including bystanders. despite widespread agreement on the importance of community engagement in biomedical research, this umbrella term includes many different goals and approaches, agreement on which is ethically ... | 2020 | 33141451 |
| the antiviral small-interfering rna pathway induces zika virus resistance in transgenic aedes aegypti. | the resurgence of arbovirus outbreaks across the globe, including the recent zika virus (zikv) epidemic in 2015-2016, emphasizes the need for innovative vector control methods. in this study, we investigated zikv susceptibility to transgenic aedes aegypti engineered to target the virus by means of the antiviral small-interfering rna (sirna) pathway. the robustness of antiviral effector expression in transgenic mosquitoes is strongly influenced by the genomic insertion locus and transgene copy nu ... | 2020 | 33142991 |
| andrographolide and its 14-aryloxy analogues inhibit zika and dengue virus infection. | andrographolide is a labdene diterpenoid with potential applications against a number of viruses, including the mosquito-transmitted dengue virus (denv). in this study, we evaluated the anti-viral activity of three 14-aryloxy analogues (zad-1 to zad-3) of andrographolide against zika virus (zikv) and denv. interestingly, one analogue, zad-1, showed better activity against both zikv and denv than the parental andrographolide. a two-dimension (2d) proteomic analysis of human a549 cells treated wit ... | 2020 | 33143016 |
| microcephaly epidemic in brazil: an earlier chapter. | identify risk factors for microcephaly and evaluate historical trends of microcephaly and arboviruses to recognize patterns and anomalies that indicate the beginning of the microcephaly epidemic associated with zika infection. | 2020 | 33144264 |
| knowledge and attitudes about zika virus infection and vaccine intent among medical students in costa rica. | zika virus (zikv) infection is a public health problem in the americas. we assessed zikv knowledge, attitudes, and future zikv vaccine intent among medical students. in this cross-sectional study, a convenience sample of medical students in san josé, costa rica, were surveyed to assess knowledge, attitudes, vaccine intent, and sources of information about zikv. knowledge and attitude scores were calculated. factors associated with vaccine intent were determined by bivariate analysis using a chi- ... | 2020 | 33146106 |
| hsv-1 and zika virus but not sars-cov-2 replicate in the human cornea and are restricted by corneal type iii interferon. | here, we report our studies of immune-mediated regulation of zika virus (zikv), herpes simplex virus 1 (hsv-1), and severe acute respiratory syndrome coronavirus 2 (sars-cov-2) infection in the human cornea. we find that zikv can be transmitted via corneal transplantation in mice. however, in human corneal explants, we report that zikv does not replicate efficiently and that sars-cov-2 does not replicate at all. additionally, we demonstrate that type iii interferon (ifn-λ) and its receptor (ifnλ ... | 2020 | 33147451 |
| multimerization of zika virus-ns5 causes ciliopathy and forces premature neurogenesis. | zika virus (zikv) is a flavivirus that infects neural tissues, causing congenital microcephaly. zikv has evolved multiple mechanisms to restrict proliferation and enhance cell death, although the underlying cellular events involved remain unclear. here we show that the zikv-ns5 protein interacts with host proteins at the base of the primary cilia in neural progenitor cells, causing an atypical non-genetic ciliopathy and premature neuron delamination. furthermore, in human microcephalic fetal bra ... | 2020 | 33147489 |
| informing epidemic (research) responses in a timely fashion by knowledge management - a zika virus use case. | the response of pathophysiological research to emerging epidemics often occurs after the epidemic and, as a consequence, has little to no impact on improving patient outcomes or on developing high-quality evidence to inform clinical management strategies during the epidemic. rapid and informed guidance of epidemic (research) responses to severe infectious disease outbreaks requires quick compilation and integration of existing pathophysiological knowledge. as a case study we chose the zika virus ... | 2020 | 33148605 |
| cd8+ t cells mediate protection against zika virus induced by an ns3-based vaccine. | zika virus (zikv) is associated with congenital malformations in infants born to infected mothers, and with guillain-barré syndrome in infected adults. development of zikv vaccines has focused predominantly on the induction of neutralizing antibodies, although a suboptimal antibody response may theoretically enhance disease severity through antibody-dependent enhancement (ade). here, we report induction of a protective anti-zikv cd8+ t cell response in the hla-b*0702 ifnar1-/- transgenic mice us ... | 2020 | 33148638 |
| burden of disease of guillain-barré syndrome in brazil before and during the zika virus epidemic 2014 - 2016. | to estimate the burden of disease of guillain-barré syndrome (gbs) in brazil in 2014, one year before the zika virus epidemic, and in 2015 and 2016 during the epidemic. | 2020 | 33151584 |
| contemporary understanding of ebola and zika virus in pregnancy. | understanding the pathophysiology, management, and prevention of emerging infectious diseases among pregnant women is imperative to achieve a successful response from the medical community. ebola and zika viruses represent infections with profound public health implications. in particular, ebola virus is associated with high case fatality and pregnancy and neonatal loss rates, while zika virus has been associated with multiple congenital anomalies; these features present critical clinical dilemm ... | 2020 | 33153665 |
| getting to the base of zika virus and microcephaly. | 2020 | 33154572 | |
| arbo-score: a rapid score for early identification of patients with imported arbovirosis caused by dengue, chikungunya and zika virus. | chikungunya (chikv), dengue (denv), and zika (zikv) viruses present significant clinical and epidemiological overlap, making an accurate and rapid diagnosis challenging. timely activation of preventive vector control measures is crucial to avoid outbreaks in non-endemic settings. diagnosis is based on combination of serological and molecular assays which could be time consuming and sometimes disappointing. | 2020 | 33158274 |
| public perceptions of threats from mosquitoes in the u.s. using online media analytics. | mosquito-borne illnesses present a public health threat. this analysis quantifies general online mosquito media, and the zika virus [zikv) specifically, from 7-20-2016 to 10-20-2018 in five u.s. geographies. the zikv sub-search comprised a shrinking share of online media about mosquitoes over time. net sentiment, numerical scoring of search result positivity/negativity bounded between -100 and +100, was assessed. mean net sentiment for the general mosquitoes search was -51; -55 for zikv. the zik ... | 2020 | 33161883 |
| electrochemical investigations for covid-19 detection-a comparison with other viral detection methods. | virus-induced infection such as sars-cov-2 is a serious threat to human health and the economic setback of the world. continued advances in the development of technologies are required before the viruses undergo mutation. the low concentration of viruses in environmental samples makes the detection extremely challenging; simple, accurate and rapid detection methods are in urgent need. of all the analytical techniques, electrochemical methods have the established capabilities to address the issue ... | 2020 | 33162783 |
| [sars-cov-2 genomic and phylodynamic analyses]. | in line with the recent ebola and zika virus epidemics, the covid-19 pandemic has led to an avalanche of genomic data. these data made it possible to better understand the origin of this virus, to date its emergence in china, but also in france, and to analyse the spread of the epidemic using techniques from the emerging field of phylodynamics. | 2020 | 33163105 |
| systematic review: fetal death reporting and risk in zika-affected pregnancies. | zika virus is linked to several adverse pregnancy outcomes. we assessed whether zika infection during pregnancy is associated with increased risk of fetal death (miscarriage, stillbirth, abortion), and whether there is incomplete reporting of such deaths. | 2020 | 33164278 |
| [microcephaly and central nervous system findings associated with congenital zika virus and other infectious etiologies in the state of rio de janeiro: crosssectional study, 2015 a 2017microcefalia y alteraciones del sistema nervioso central relacionadas con la infección congénita por el virus del zika y otras etiologías infecciosas en el estado de río de janeiro: estudio transversal, 2015-2017]. | to describe the clinical and epidemiological profile of cases with confirmed microcephaly or central nervous system (cns) findings associated with congenital zika virus infection and other infectious etiologies in the state of rio de janeiro, brazil, from november 2015 to july 2017. | 2020 | 33165406 |
| immune reactivity of a 20-mer peptide representing the zika e glycan loop involves the antigenic determinants e-152/156/158. | mosquito-borne zika virus (zikv) causes a severe congenital syndrome and neurological disorders in humans. with the aim to develop a live-attenuated zikv strain, we generated a chimeric viral clone zikalivax with african mr766-niid strain as backbone and the envelope e protein of epidemic brazilian beh810915 strain. the mr766-niid residues e-t152/i156/y158 were introduced into beh810915 e protein leading to a nonglycosylated zikalivax. recently, we reported that the residues e-152/156/158 that a ... | 2020 | 33167511 |
| can zika virus infection in high risk pregnant women be differentiated on the basis of symptoms? | zika virus (zikv) infection in pregnancy is associated with congenital neurological abnormalities. our understanding of the full clinical spectrum of zikv infection is incomplete. using data from this prospective cohort study consisting of 650 women attending a high-risk pregnancy clinic during the zika virus outbreak in brazil, we investigated the extent to which specific symptoms can be utilized to differentiate zikv-infected pregnant women from those with other pregnancy-related problems. all ... | 2020 | 33167566 |
| evolution of ocular defects in infant macaques following in utero zika virus infection. | congenital zika syndrome (czs) is associated with microcephaly and various neurological, musculoskeletal, and ocular abnormalities, but the long-term pathogenesis and postnatal progression of ocular defects in infants are not well characterized. rhesus macaques are superior to rodents as models of czs because they are natural hosts of the virus and share similar immune and ocular characteristics, including blood-retinal barrier characteristics and the unique presence of a macula. using a previou ... | 2020 | 33180748 |
| single amino acid mutations affect zika virus replication in vitro and virulence in vivo. | the 2014-2016 zika virus (zikv) epidemic in the americas resulted in large deposits of next-generation sequencing data from clinical samples. this resource was mined to identify emerging mutations and trends in mutations as the outbreak progressed over time. information on transmission dynamics, prevalence, and persistence of intra-host mutants, and the position of a mutation on a protein were then used to prioritize 544 reported mutations based on their ability to impact zikv phenotype. using t ... | 2020 | 33198111 |
| zika virus and host interactions: from the bench to the bedside and beyond. | before the emergence of sars-cov-2 (severe acute respiratory syndrome coronavirus 2), the causative agent of the current covid-19 (coronavirus disease 2019) pandemic [...]. | 2020 | 33198165 |
| biological characteristics and patterns of codon usage evolution for the african genotype zika virus. | we investigated temporal trends of codon usage changes for different host species to determine their importance in zika virus (zikv) evolution. viral spillover resulting from the potential of codon adaptation to host genome was also assessed for the african genotype zikv in comparison to the asian genotype. to improve our understanding on its zoonotic maintenance, we evaluated in vitro the biological properties of the african genotype zikv in vertebrate and mosquito cell lines. analyses were per ... | 2020 | 33202554 |
| novel hepaci- and pegi-like viruses in native australian wildlife and non-human primates. | the flaviviridae family of positive-sense rna viruses contains important pathogens of humans and other animals, including zika virus, dengue virus, and hepatitis c virus. the flaviviridae are currently divided into four genera-hepacivirus, pegivirus, pestivirus, and flavivirus-each with a diverse host range. members of the genus hepacivirus are associated with an array of animal species, including humans, non-human primates, other mammalian species, as well as birds and fish, while the closely r ... | 2020 | 33240526 |
| low aedes aegypti vector competence for zika virus from viremic rhesus macaques. | despite worldwide efforts to understand the transmission dynamics of zika virus (zikv), scanty evaluation has been made on the vector competence of aedes aegypti fed directly on viremic human and non-human primates (nhps). we blood-fed ae. aegypti from two districts in rio de janeiro on six zikv infected pregnant rhesus macaques at several time points, half of which were treated with sofosbuvir (sof). mosquitoes were analyzed for vector competence after 3, 7 and 14 days of incubation. although v ... | 2020 | 33255150 |
| fetal rhesus monkey first trimester zika virus infection impacts cortical development in the second and third trimesters. | zika virus is a teratogen similar to other neurotropic viruses, notably cytomegalovirus and rubella. the goal of these studies was to address the direct impact of zika virus on fetal development by inoculating early gestation fetal rhesus monkeys using an ultrasound-guided approach (intraperitoneal vs. intraventricular). growth and development were monitored across gestation, maternal samples collected, and fetal tissues obtained in the second trimester or near term. although normal growth and a ... | 2021 | 33341889 |
| transcriptional signatures of zika virus infection in astrocytes. | astrocytes are an early and important target of zika virus (zikv) infection in the developing brain, but the impacts of infection on astrocyte function remain controversial. given that nonhuman primate (nhp) models of zikv infection replicate aspects of neurologic disease seen in human infections, we cultured primary astrocytes from the brain tissue of infant rhesus macaques and then infected the cells with asian or african lineage zikv to identify transcriptional patterns associated with infect ... | 2021 | 33405202 |
| clinical and preclinical evidence for adverse neurodevelopment after postnatal zika virus infection. | although the zika virus (zikv) typically causes mild or no symptoms in adults, during the 2015-2016 outbreak, zikv infection in pregnancy resulted in a spectrum of diseases in infants, including birth defects and neurodevelopmental disorders identified in childhood. while intense clinical and basic science research has focused on the neurodevelopmental outcomes of prenatal zikv infection, less is known about the consequences of infection during early life. considering the neurotropism of zikv an ... | 2021 | 33445671 |
| two immunogenic recombinant protein vaccine candidates showed disparate protective efficacy against zika virus infection in rhesus macaques. | zika virus (zikv) infection has caused major public health problems recently. to develop subunit vaccines for zikv, we have previously constructed recombinant zikv envelope protein domain iii (ediii), and the entire ectodomain (e80, which comprises edi, edii and ediii), as vaccine candidates and showed both of them being immunogenic and protective in murine models. in this follow-up study, we compared these vaccine candidates in non-human primates. both of them elicited neutralizing antibody res ... | 2021 | 33451779 |
| genome-wide identification of aedes albopictus long noncoding rnas and their association with dengue and zika virus infection. | the asian tiger mosquito, aedes albopictus (ae. albopictus), is an important vector that transmits arboviruses such as dengue (denv), zika (zikv) and chikungunya virus (chikv). long noncoding rnas (lncrnas) are known to regulate various biological processes. knowledge on ae. albopictus lncrnas and their functional role in virus-host interactions are still limited. here, we identified and characterized the lncrnas in the genome of an arbovirus vector, ae. albopictus, and evaluated their potential ... | 2021 | 33481791 |
| dengue, yellow fever, zika and chikungunya epidemic arboviruses in brazil: ultrastructural aspects. | the impact of arbovirus cocirculation in brazil is unknown. dengue virus (denv) reinfection may result in more intense viraemia or immunopathology, leading to more severe disease. the zika virus (zikv) epidemic in the americas provided pathogenicity evidence that had not been previously observed in flavivirus infections. in contrast to other flaviviruses, electron microscopy studies have shown that zikv may replicate in viroplasm-like structures. flaviviruses produce an ensemble of structurally ... | 2021 | 33566939 |
| a bacterially-expressed recombinant envelope protein from usutu virus induces neutralizing antibodies in rabbits. | recently, an emerging flavivirus, usutu virus (usuv), has caused an epidemic among birds in europe, resulting in a massive die-off in eurasian blackbirds. currently found only in europe and africa, it can be envisioned that usutu virus will follow the path of other flaviviruses, like west nile virus and zika virus, and will spread via its mosquito vectors and bird hosts to other parts of the world. several cases of human infections by usutu virus have already been published. anticipating this sp ... | 2021 | 33669414 |
| zika virus-like particle vaccine protects ag129 mice and rhesus macaques against zika virus. | zika virus (zikv), a mosquito-borne flavivirus, is a re-emerging virus that constitutes a public health threat due to its recent global spread, recurrent outbreaks, and infections that are associated with neurological abnormalities in developing fetuses and guillain-barré syndrome in adults. to date, there are no approved vaccines against zikv infection. various preclinical and clinical development programs are currently ongoing in an effort to bring forward a vaccine for zikv. | 2021 | 33711018 |
| an integrated molecular approach to untangling host-vector-pathogen interactions in mosquitoes (diptera: culicidae) from sylvan communities in mexico. | there are ~240 species of culicidae in mexico, of which some are vectors of arthropod-borne viruses such as zika virus, dengue virus, chikungunya virus, and west nile virus. thus, the identification of mosquito feeding preferences is paramount to understanding of vector-host-pathogen interactions that, in turn, can aid the control of disease outbreaks. typically, dna and rna are extracted separately for animal (insects and blood meal hosts) and viral identification, but this study demonstrates t ... | 2021 | 33778029 |
| subgenomic flavivirus rna (sfrna) associated with asian lineage zika virus identified in three species of ugandan bats (family pteropodidae). | serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. we hypothesized that prior infection with zikv could be confirmed through the presence of subgenomic flavivirus rna (sfrna) of the 3' untranslated region (utr), which persists in tissues due to xrn-1 stalling during rna decay. we amplified zikv sfrna but not ns5 from three experimentally-infected jamaican fruit bats, sup ... | 2021 | 33863991 |
| a high throughput reporter virus particle microneutralization assay for quantitation of zika virus neutralizing antibodies in multiple species. | zika virus is a flavivirus, transmitted via aedes mosquitos, that causes a range of symptoms including zika congenital syndrome. zika has posed a challenging situation for health, public and economic sectors of affected countries. to quantitate zika virus neutralizing antibody titers in serum samples, we developed a high throughput plate based zika virus reporter virus particle (rvp) assay that uses an infective, non-replicating particle encoding zika virus surface proteins and capsid (cprme) an ... | 2021 | 33891631 |
| infection, dissemination, and transmission efficiencies of zika virus in aedes aegypti after serial passage in mosquito or mammalian cell lines or alternating passage in both cell types. | zika virus (zikv) is an arthropod-borne virus (arbovirus) with an urban transmission cycle that primarily involves humans and aedes aegypti. evidence suggests that the evolution of some arboviruses is constrained by their dependency on alternating between disparate (vertebrate and invertebrate) hosts. the goals of this study are to compare the genetic changes that occur in zikv after serial passaging in mosquito or vertebrate cell lines or alternate passaging in both cell types and to compare th ... | 2021 | 34006306 |
| african-lineage zika virus replication dynamics and maternal-fetal interface infection in pregnant rhesus macaques. | following the zika virus (zikv) outbreak in the americas, zikv was causally associated with microcephaly and a range of neurological and developmental symptoms, termed congenital zika syndrome (czs). the viruses responsible for this outbreak belonged to the asian lineage of zikv. however, in vitro and in vivo studies assessing the pathogenesis of african-lineage zikv demonstrated that african-lineage isolates often replicated to high titers and caused more-severe pathology than asian-lineage iso ... | 2021 | 34076485 |
| early embryonic loss following intravaginal zika virus challenge in rhesus macaques. | zika virus (zikv) is an arthropod-borne virus (arbovirus) and is primarily transmitted by aedes species mosquitoes; however, zikv can also be sexually transmitted. during the initial epidemic and in places where zikv is now considered endemic, it is difficult to disentangle the risks and contributions of sexual versus vector-borne transmission to adverse pregnancy outcomes. to examine the potential impact of sexual transmission of zikv on pregnancy outcome, we challenged three rhesus macaques (m ... | 2021 | 34079560 |
| systemic inflammation, innate immunity and pathogenesis after zika virus infection in cynomolgus macaques are modulated by strain-specificity within the asian lineage. | zika virus (zikv) is an emerging arbovirus with recent global expansion. historically, zikv infections with asian lineages have been associated with mild disease such as rash and fever. however, recent asian sub-lineages have caused outbreaks in the south pacific and latin america with increased prevalence of neurological disorders in infants and adults. asian sub-lineage differences may partially explain the range of disease severity observed. however, the effect of asian sub-lineage difference ... | 2021 | 34120576 |
| the liver x receptor agonist lxr 623 restricts flavivirus replication. | the vector-borne flaviviruses (vbfvs) are well known for causing great misery and death in humans worldwide. the vbfvs include those transmitted by mosquitos, such as zika virus (zikv), dengue virus; and those transmitted by ticks including the tick-borne flavivirus serocomplex and powassan virus (powv). two of our recent reports showed that intracranial powv infection in the reservoir host, peromyscus leucopus, was restricted and caused no overt clinical disease. several modes of analyses sugge ... | 2021 | 34162308 |
| detection of virus-related sequences associated with potential etiologies of hepatitis in liver tissue samples from rats, mice, shrews, and bats. | hepatitis is a major global health concern. however, the etiology of 10-20% hepatitis cases remains unclear. some hepatitis-associated viruses, like the hepatitis e virus, are zoonotic pathogens. rats, shrews, and bats are reservoirs for many zoonotic pathogens. therefore, understanding the virome in the liver of these animals is important for the investigation of the etiologies of hepatitis and monitoring the emerging zoonotic viruses. in this study, viral metagenomics and pcr methods were used ... | 2021 | 34177835 |
| recapitulating zika virus infection in vagina of tree shrew (tupaia belangeri). | sexual transmission of zika virus (zikv) elevates the risk of its dissemination in the female reproductive tract and causes a serious threat to the fetus. however, the available animal models are not appropriate to investigate sexual transmission, dynamics of zikv infection, replication, and shedding. the use of tree shrew as a small animal model of zikv vaginal infection was assessed in this study. a total of 23 sexually mature female tree shrews were infected with zikv gz01 via the intravagina ... | 2021 | 34249779 |