Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| post-travel screening of asymptomatic long-term travelers to the tropics for intestinal parasites using molecular diagnostics. | the incidence of asymptomatic travel-related parasitic infection is uncertain. previous studies did not distinguish new incident infections, from past infections. regardless of symptoms, we performed multiplex real-time polymerase chain reaction on pre- and post-travel stool samples of dutch long-term travelers to the (sub)tropics. serological screening for schistosoma spp. was only performed in travelers to sub-saharan africa. in total, 679 travelers were included in the study. the follow-up ra ... | 2014 | 24615130 |
| proteomic identification of s-nitrosylated proteins in the parasite entamoeba histolytica by resin-assisted capture: insights into the regulation of the gal/galnac lectin by nitric oxide. | entamoeba histolytica is a gastrointestinal protozoan parasite that causes amebiasis, a disease which has a worldwide distribution with substantial morbidity and mortality. nitrosative stress, which is generated by innate immune cells, is one of the various environmental challenges that e. histolytica encounters during its life cycle. although the effects of nitric oxide (no) on the regulation of gene expression in this parasite have been previously investigated, our knowledge on s-nitrosylated ... | 2014 | 24626316 |
| analysis of u3 snorna and small subunit processome components in the parasitic protist entamoeba histolytica. | in the early branching parasitic protist entamoeba histolytica, pre-rrna synthesis continues when cells are subjected to growth stress, but processing slows down and unprocessed pre-rrna accumulates. to gain insight into the regulatory mechanisms leading to accumulation, it is necessary to define the pre-rrna processing machinery in e. histolytica. we searched the e. histolytica genome sequence for homologs of the ssu processome, which contains the u3snorna, and 72 proteins in yeast. we could id ... | 2014 | 24631428 |
| prevalence of intestinal protozoan infections among orang asli schoolchildren in pos senderut, pahang, malaysia. | the current study determined the prevalence of intestinal protozoan infections among the orang asli schoolchildren in pos senderut, pahang, malaysia. the overall intestinal protozoan infection rate was 85% (261 out of 307). the highest prevalence rates were due to entamoeba coli (24.4%), giardia lamblia (21.8%), blastocystis hominis (21.2%) and entamoeba histolytica (15.0%). the prevalence of iodamoeba butschlii was only 2.9%. among the positive samples, mixed infection with b. hominis and e. hi ... | 2013 | 24640856 |
| natural infection of baboons by entamoeba histolytica elicits anti- gal-lectin heavy subunit iga and igg antibodies with shared epitope specificity to that of humans. | non-human primates, such as baboons (papio hamadryas anubis), are natural hosts for entamoeba species; infections can be asymptomatic or result in invasive lethal disease. it was sought to determine whether following natural infection by entamoeba. histolytica, baboon anti-amebic antibodies recognized native gallectin, a recombinant portion of the lectin heavy subunit (designated lc3) and specific heavy subunit epitopes; we compared the specificity of anti-amebic antibodies from baboons to that ... | 2013 | 24640872 |
| isolation of intestinal parasites of public health importance from cockroaches (blattella germanica) in jimma town, southwestern ethiopia. | cockroaches are claimed to be mechanical transmitters of disease causing microorganisms such as intestinal parasites, bacteria, fungi, and viruses. this study assessed the potential of the german cockroach blattella germanica in the mechanical transmission of intestinal parasites of public health importance. a total of 2010 cockroaches were collected from 404 households in jimma town, southwestern ethiopia. all the collected cockroaches were identified to species as b. germanica. the contents of ... | 2014 | 24649356 |
| [prevalence of intestinal microsporidia and other intestinal parasites in hiv positive patients from maracaibo, venezuela]. | to detect the presence of microsporidia and other enteric parasites in patients with hivaids of the autonomous services university hospital of maracaibo (sahum), where there are no previous studies in this field. | 2014 | 24652209 |
| parasitic infections of the appendix as a cause of appendectomy in adult patients. | assessment of frequency and clinical findings of parasitic infections for etiology of acute appendicitis. | 2014 | 24659695 |
| amebic liver abscess: fine needle aspiration diagnosis. | to describe the findings in fine needle aspiration (fna) of an amebic liver abscess (ala). | 2014 | 24662424 |
| the awareness of health professionals in diagnostic techniques for intestinal parasites in gaza strip, palestine. | diagnosis of intestinal parasites still depends on conventional methods in gaza strip hospitals and private laboratories. | 2014 | 24669336 |
| study of opportunistic intestinal parasitic infections in human immunodeficiency virus/acquired immunodeficiency syndrome patients. | intestinal parasites predominantly coccidian parasites are a common cause for diarrhea in human immunodeficiency virus (hiv)-positive patients. | 2013 | 24672179 |
| differential diagnosis of entamoeba spp. in clinical stool samples using sybr green real-time polymerase chain reaction. | amoebiasis, a disease caused by entamoeba histolytica, is usually diagnosed by microscopic examination, which does not differentiate the morphologically identical species of the e. histolytica/e. dispar complex. furthermore, morphologically similar species such as entamoeba hartmanni contribute to misidentification. therefore, there is a need for more sensitive and specific methods. this study standardized a multiplex real-time pcr system for e. histolytica and e. dispar and a single real-time p ... | 2014 | 24693242 |
| production of recombinant entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. | amoebic liver abscess (ala) is the most common clinical manifestation of extraintestinal amoebiasis especially in developing countries, causing up to 100 000 fatal cases annually. accurate and early diagnosis is important to prevent the disease complications, however its diagnosis still poses many challenges due to the limitations of the available detection tools. pyruvate phosphate dikinase (ppdk), an excretory-secretory protein of e. histolytica, has been reported as a potential diagnostic mar ... | 2014 | 24708664 |
| trogocytosis by entamoeba histolytica contributes to cell killing and tissue invasion. | entamoeba histolytica is the causative agent of amoebiasis, a potentially fatal diarrhoeal disease in the developing world. the parasite was named "histolytica" for its ability to destroy host tissues, which is probably driven by direct killing of human cells. the mechanism of human cell killing has been unclear, although the accepted model was that the parasites use secreted toxic effectors to kill cells before ingestion. here we report the discovery that amoebae kill by ingesting distinct piec ... | 2014 | 24717428 |
| unusual findings in appendectomy specimens of adults: retrospective analyses of 1466 patients and a review of literature. | diseases and tumors of the appendix vermiformis are very rare, except acute appendicitis. | 2014 | 24719727 |
| naegleria fowleri: contact-dependent secretion of electrondense granules (edg). | the free living amoeba naegleria fowleri is pathogenic to humans but also to other mammalians. these amoebae may invade the nasal mucosa and migrate into the brain causing cerebral hemorrhagic necrosis, a rapidly fatal infection. knowledge of the cytolytic mechanism involved in the destruction of brain tissues by naegleria trophozoites is limited. in other amoebic species, such as entamoeba histolytica, we have previously reported the possible lytic role of small cytoplasmic components endowed w ... | 2014 | 24721258 |
| proteomic profiling reveals that ehpc4 transcription factor induces cell migration through up-regulation of the 16-kda actin-binding protein ehabp16 in entamoeba histolytica. | actin cytoskeleton is an essential structure involved in cell migration and invasion in parasites. in entamoeba histolytica, the protozoan parasite causing human amoebiasis, the mechanisms underlying the expression of migration-related genes are poorly understood. here, we investigated the biological effects of ectopic overexpression of ehpc4 (positive coactivator 4) in cell migration of e. histolytica trophozoites. using differential in gel two-dimensional electrophoresis, 33 modulated proteins ... | 2014 | 24721673 |
| water-soluble ruthenium complexes bearing activity against protozoan parasites. | parasitic illnesses are major causes of human disease and misery worldwide. among them, both amebiasis and chagas disease, caused by the protozoan parasites, entamoeba histolytica and trypanosoma cruzi, are responsible for thousands of annual deaths. the lack of safe and effective chemotherapy and/or the appearance of current drug resistance make the development of novel pharmacological tools for their treatment relevant. in this sense, within the framework of the medicinal inorganic chemistry, ... | 2014 | 24740394 |
| genital infection caused by entamoeba histolytica confirmed by polymerase chain reaction analyses. | entamoeba histolytica is estimated to infect approximately 1% of the global population. in japan, the prevalence of amebic dysentery has been increasing, with more than 800 patients newly diagnosed annually. however, genital infection with e. histolytica is uncommon even in endemic areas. we present a case of vaginitis caused by e. histolytica. a 50-year-old japanese woman without history of overseas travel presented to a nearby clinic with increased vaginal discharge. she had hemorrhagic erosio ... | 2014 | 24750396 |
| amoebicidal activity of essential oil of dysphania ambrosioides (l.) mosyakin & clemants in an amoebic liver abscess hamster model. | amebiasis is a parasitic disease that extends worldwide and is a public health problem in developing countries. metronidazole is the drug recommended in the treatment of amebiasis, but its contralateral effects and lack of continuity of treatment induce low efficiency, coupled with the appearance of resistant amoebic strains. therefore, the search of new compounds with amoebicidal activity is urgent and important. in this study, we evaluated the in vitro and in vivo antiamoebic activity of the e ... | 2014 | 24757495 |
| intestinal parasitic infestation in combatants and their families: a hospital-based study in mid-western regional police hospital, nepal. | to find out the scenario of intestinal parasitic infestation in combatants and their families in the setting of mid-western regional police hospital (mwrph), nepal. | 2014 | 24762341 |
| hepatopleuropulmonary amoebiasis--a case report. | amoebiasis is a food-borne protozoan infection, caused by entamoeba histolytica. here a case of hepatopleuropulmonary amoebiasis, which was detected after fibre-optic bronchoscopy is reported. bronchial aspirate showed trophozoites of entamoeba histolytica. the patient was treated with tinidazole and responded favourably. | 2013 | 24765700 |
| new evidence of entamoeba histolytica infections in pre-columbian and colonial cemeteries in the caribbean. | elisa was used to test the presence of the human pathogenic amoeba entamoeba histolytica in archaeological samples from 2 cemeteries in guadeloupe, in the caribbean. results show that 15.9% of the population was infected during the colonial period, a value comparable to the current rates observed in humans in adjacent countries. this study also confirms the presence of the current strain of e. histolytica in pre-columbian south america before the european colonization in the 15th century and rai ... | 2014 | 24766261 |
| conformational changes induced by detergents during the refolding of chemically denatured cysteine protease ppehcp-b9 from entamoeba histolytica. | ehcp-b9, a cysteine protease (cp) involved in entamoeba histolytica virulence, is a potential target for disease diagnosis and drug design. after purification from inclusion bodies produced in escherichia coli, the recombinant ehcp-b9 precursor (ppehcp-b9) can be refolded using detergents as artificial chaperones. however, the conformational changes that occur during ppehcp-b9 refolding remain unknown. here, we comprehensively describe conformational changes of ppehcp-b9 that are induced by vari ... | 2014 | 24769005 |
| assessment of gastrointestinal parasites in wild chimpanzees (pan troglodytes troglodytes) in southeast cameroon. | we tested 114 faecal samples from wild simian immunodeficiency virus (siv)-positive (n = 43) and siv-negative (n = 71) chimpanzees (pan troglodytes troglodytes) in southeast cameroon for the presence of gastrointestinal parasites by direct smear. we observed cysts from different protozoa (entamoeba coli and entamoeba histolytica / entamoeba dispar, endolimax nana, iodamoeba butschlii, chilomastix mesnili, balantidium coli and blastocystis cells) and trophozoites from troglodytella abrassarti and ... | 2014 | 24781023 |
| development of a loop-mediated isothermal amplification assay for detection of trichomonas vaginalis. | a loop-mediated isothermal amplification (lamp) assay targeting the 2-kbp repeated dna species-specific sequence was developed for detection of trichomonas vaginalis, the causative agent of trichomoniasis. the analytical sensitivity and specificity of the lamp assay were evaluated using pooled genital swab and urine specimens, respectively, spiked with t. vaginalis trophozoites. genital secretion and urine did not inhibit the detection of the parasite. the sensitivity of the lamp was 10-1000 tim ... | 2014 | 24792836 |
| effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review. | melatonin, a hormone secreted by the pineal gland, works directly and indirectly as a free radical scavenger. its other physiological or pharmacological activities could be dependent or independent of receptors located in different cells, organs, and tissues. in addition to its role in promoting sleep and circadian rhythms regulation, it has important immunomodulatory, antioxidant, and neuroprotective effects suggesting that this indole must be considered as a therapeutic alternative against inf ... | 2014 | 24811367 |
| the macrophage migration inhibitory factor homolog of entamoeba histolytica binds to and immunomodulates host macrophages. | the host inflammatory response contributes to the tissue damage that occurs during amebic colitis, with tumor necrosis factor alpha (tnf-α) being a key mediator of the gut inflammation observed. mammalian macrophage migration inhibitory factor (mif) is a proinflammatory cytokine that plays an important role in the exacerbation of a wide range of inflammatory diseases, including colitis. we identified a mif gene homolog in the entamoeba histolytica genome, raising the question of whether e. histo ... | 2014 | 24818664 |
| vagotomy induces deregulation of the inflammatory response during the development of amoebic liver abscess in hamsters. | the parasympathetic nervous system modulates the immune response in the abdominal-pelvic gut through the vagus nerve, which releases acetylcholine. this endogenous ligand acts on α7 nicotinic receptors expressed on immune cells. | 2015 | 24819982 |
| hsp90 inhibitors as new leads to target parasitic diarrheal diseases. | entamoeba histolytica and giardia lamblia are anaerobic protozoan parasites that cause amebiasis and giardiasis, two of the most common diarrheal diseases worldwide. current therapy relies on metronidazole, but resistance has been reported and the drug has significant adverse effects. therefore, it is critical to search for effective, better-tolerated antiamebic and antigiardial drugs. we synthesized several examples of a recently reported class of hsp90 inhibitors and evaluated these compounds ... | 2014 | 24820073 |
| peroxynitrite and peroxiredoxin in the pathogenesis of experimental amebic liver abscess. | the molecular mechanisms by which entamoeba histolytica causes amebic liver abscess (ala) are still not fully understood. amebic mechanisms of adherence and cytotoxic activity are pivotal for amebic survival but apparently do not directly cause liver abscess. abundant evidence indicates that chronic inflammation (resulting from an inadequate immune response) is probably the main cause of ala. reports referring to inflammatory mechanisms of liver damage mention a repertoire of toxic molecules by ... | 2014 | 24822193 |
| immuno-pcr for detection of giardia lamblia cysts in water. | giardia lamblia cysts at low concentrations were detected in water samples using a highly sensitive immunological-pcr (ipcr) method. magnetic gold particles were precoated with monoclonal anti-giardia antibodies, and giardia lamblia cysts ranging from 1 to 100 cysts were diluted in 500 microl of water. a biotinylated detection antibody bound to the g. lamblia cysts was then linked by a streptavidin bridge to biotinylated giardia-reporter dna. after extensive washing, reporter dna was released by ... | 2014 | 24830167 |
| [ileocecal ameboma: a case diagnosed by pathology and molecular biology]. | colonic ameboma is a rare benign inflammatory tumor due to the infection by entamoeba histolytica and poses frequently the problem of colon cancer. we report a case of a 52 year-old patient who presented a cecal amoeboma revealed by a painful mass in the right iliac fossa. radiologic and endoscopic examinations depicted a parietal thickening of the right colon and the cecum. a presumptive diagnosis of colon cancer was firstly discussed. confirmation of ameboma was made on pathological examinatio ... | 2012 | 24834662 |
| epidemiological features of intestinal infection with entamoeba histolytica in taiwan, 2002-2010. | amebiasis remains an important public health problem worldwide, and immigration and an increase in international travel have affected the incident cases of the disease. the purpose of this study was to assess the prevalence of entamoeba histolytica in taiwan between 2002 and 2010. we analyzed data from surveillance programs run by the centers for disease control, taiwan (taiwan cdc), and only laboratory-confirmed cases were analyzed. in total, 1796 cases with e. histolytica infections were inclu ... | 2014 | 24837854 |
| parasitic infections based on 320 clinical samples submitted to hanyang university, korea (2004-2011). | we analyzed 320 clinical samples of parasitic infections submitted to the department of environmental biology and medical parasitology, hanyang university from january 2004 to june 2011. they consisted of 211 nematode infections, 64 trematode or cestode infections, 32 protozoan infections, and 13 infections with arthropods. the nematode infections included 67 cases of trichuriasis, 62 of anisakiasis (anisakis sp. and pseudoterranova decipiens), 40 of enterobiasis, and 24 of ascariasis, as well a ... | 2014 | 24850969 |
| entamoeba dispar: a rare case of enteritis in a patient living in a nonendemic area. | entamoeba dispar, a common noninvasive parasite, is indistinguishable in its cysts and trophozoite forms from entamoeba histolytica, the cause of invasive amebiasis, by microscopy. to differentiate the two species seems to be a problem for laboratory diagnosis. recent experimental studies showed that e. dispar can be considered pathogenic too. we present a rare case of enteritis due to e. dispar. | 2014 | 24851190 |
| structural and mutational analysis of amino acid residues involved in atp specificity of escherichia coli acetate kinase. | acetate kinase (ak) generally utilizes atp as a phosphoryl donor, but ak from entamoeba histolytica (ppi-ehiak) uses pyrophosphate (ppi), not atp, and is ppi-specific. the determinants of the phosphoryl donor specificity are unknown. here, we inferred 5 candidate amino acid residues associated with this specificity, based on structural information. each candidate residue in escherichia coli atp-specific ak (atp-ecoak), which is unable to use ppi, was substituted with the respective ppi-ehiak ami ... | 2014 | 24856051 |
| entamoeba histolytica and e. dispar calreticulin: inhibition of classical complement pathway and differences in the level of expression in amoebic liver abscess. | the role of calreticulin (crt) in host-parasite interactions has recently become an important area of research. information about the functions of calreticulin and its relevance to the physiology of entamoeba parasites is limited. the present work demonstrates that crt of both pathogenic e. histolytica and nonpathogenic e. dispar species specifically interacted with human c1q inhibiting the activation of the classical complement pathway. using recombinant ehcrt protein, we demonstrate that crt i ... | 2014 | 24860808 |
| parasitic infections on the shore of lake victoria (east africa) detected by mini-flotac and standard techniques. | helminths and protozoa infections pose a great burden especially in developing countries, due to morbidity caused by both acute and chronic infection. the aim of our survey was to analyze the intestinal parasitic burden in communities from mwanza region, tanzania. | 2014 | 24865791 |
| etiology of childhood diarrhea after rotavirus vaccine introduction: a prospective, population-based study in nicaragua. | nicaragua was the first developing nation to implement routine immunization with the pentavalent rotavirus vaccine (rv5). in this rv5-immunized population, understanding infectious etiologies of childhood diarrhea is necessary to direct diarrhea treatment and prevention efforts. | 2014 | 24879131 |
| intestinal parasitoses in a tertiary-care hospital located in a non-endemic setting during 2006-2010. | the aim of this study was to assess the epidemiology of intestinal parasitoses during a 5-year period in patients attending a tertiary-care hospital in a non-endemic setting. | 2014 | 24886502 |
| the flavonoid (-)-epicatechin affects cytoskeleton proteins and functions in entamoeba histolytica. | human amoebiasis is an intestinal disease with a global distribution. due to reports of parasite resistance or susceptibility reduction to metronidazole treatment, there is a renewed interest for the search of new molecules with antiamoebic activity. the flavonoid (-)-epicatechin that was isolated from the mexican medicinal plant geranium mexicanum hbk has an in vitro activity against e. histolytica trophozoites, however its molecular effects have been poorly documented. using a proteomic approa ... | 2014 | 24887480 |
| ultrastructural characteristics and molecular identification of entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity. | protozoan parasites of the genus entamoeba infect many classes of vertebrates and are primarily classified based on morphological criteria. to date, only a few species have been proven to cause disease. here, we examined the pathology of infected pigs with hemorrhage and detected entamoeba parasites. isolates were characterized genetically and ultrastructurally to identify the species. histopathologically, bleeding and thrombus formation were seen only in the large intestine mucosa, where a larg ... | 2014 | 24894081 |
| ameboma: an unusual cause of gastrointestinal bleeding during severe leptospirosis. | severe leptospirosis occurs mainly in a tropical environment and includes icterus, acute renal failure and hemorrhages. these bleedings, which are mainly a consequence of acute homeostatic disturbances, can also reveal simultaneous diseases. coinfections with other tropical diseases have been previously reported during leptospirosis. to our knowledge, invasive amebiasis, which can induce gastrointestinal bleedings, has never been described in the course of severe leptospirosis. | 2014 | 24894109 |
| ligand heterogeneity of the cysteine protease binding protein family in the parasitic protist entamoeba histolytica. | lysosomal soluble proteins are targeted to endosomes and lysosomes by specific receptors resident in the endoplasmic reticulum and/or the golgi apparatus. the enteric protozoan parasite entamoeba histolytica has a novel class of lysosomal targeting receptors, named the cysteine protease binding protein family (cpbf). among 11 cpbfs (cpbf1-11), ligands for three members, cpbf1, cpbf6 and cpbf8, were previously shown to be cysteine proteases, α- and γ- amylases, and β-hexosaminidase and lysozymes, ... | 2014 | 24907554 |
| atomic resolution crystal structure of vclmwptp-1 from vibrio cholerae o395: insights into a novel mode of dimerization in the low molecular weight protein tyrosine phosphatase family. | low molecular weight protein tyrosine phosphatase (lmwptp) is a group of phosphotyrosine phosphatase ubiquitously found in a wide range of organisms ranging from bacteria to mammals. dimerization in the lmwptp family has been reported earlier which follows a common mechanism involving active site residues leading to an enzymatically inactive species. here we report a novel form of dimerization in a lmwptp from vibrio cholera 0395 (vclmwptp-1). studies in solution reveal the existence of the dime ... | 2014 | 24909685 |
| intestinal parasitic infections among prison inmates and tobacco farm workers in shewa robit, north-central ethiopia. | intestinal parasitic infections (ipis) particularly soil-transmitted helminthiasis (sth) and schistosomiasis are among neglected tropical diseases (ntds) globally. apart from being associated with anemia, malabsorption and retarded cognitive development these diseases are complicating the clinical picture of more serious infections like hiv, tb and malaria. renewed and up-to-date information on the epidemiology of ipis in more vulnerable groups such as irrigated-farm workers and prisoners would ... | 2014 | 24926687 |
| destabilization domain approach adapted for regulated protein expression in the protozoan parasite entamoeba histolytica. | a plethora of information has been gained by sequencing the genome of the human parasite entamoeba histolytica, however a lack of robust genetic tools hampers experimental elucidation of gene functions. we adapted the destabilization domain approach for modulation of protein levels in e. histolytica using the destabilization domains of fk506 binding protein (ddfkbp) and dihydrofolate reductase (dddhfr), respectively. in our studies, the ddfkbp appears to be more tightly regulated than dddhfr, wi ... | 2014 | 24929134 |
| inferior vena cava thrombosis in a case of amoebic liver abscess: is hypercomplementemia responsible for this rare entity? | among the liver abscesses, thrombosis of the inferior vena cava (ivc) has been reported mainly in amoebic liver abscess (ala) caused by entamoeba histolytica (e.h). it is an unusual complication especially in paediatric age group. association of hypercomplementemia and ivc thrombosis has not been discussed previously. published data suggest that e.h can activate the complement system and can cause hypercomplementemia. a very few studies suggest that complement activation and hypercomplementemia ... | 2014 | 24943781 |
| metabolomic analysis of entamoeba: applications and implications. | entamoeba histolytica is an enteric protozoan parasite that causes hemorrhagic dysentery and extraintestinal abscesses in millions of inhabitants of endemic areas. the genome of e. histolytica has already been sequenced and used to predict the metabolic potential of the organism. since nearly 56% of the e. histolytica genes remain unannotated, correlative 'omics' analyses of genomics, transcriptomics, proteomics, and biochemical metabolic profiling are essential in uncovering new, or poorly unde ... | 2014 | 24950028 |
| ip6k structure and the molecular determinants of catalytic specificity in an inositol phosphate kinase family. | inositol trisphosphate kinases (ip3ks) and inositol hexakisphosphate kinases (ip6ks) each regulate specialized signalling activities by phosphorylating either insp3 or insp6 respectively. the molecular basis for these different kinase activities can be illuminated by a structural description of ip6k. here we describe the crystal structure of an entamoeba histolytica hybrid ip6k/ip3k, an enzymatic parallel to a 'living fossil'. through molecular modelling and mutagenesis, we extrapolated our find ... | 2014 | 24956979 |
| analysis of the epithelial damage produced by entamoeba histolytica infection. | entamoeba histolytica is the causative agent of human amoebiasis, a major cause of diarrhea and hepatic abscess in tropical countries. infection is initiated by interaction of the pathogen with intestinal epithelial cells. this interaction leads to disruption of intercellular structures such as tight junctions (tj). tj ensure sealing of the epithelial layer to separate host tissue from gut lumen. recent studies provide evidence that disruption of tj by the parasitic protein ehcpadh112 is a prere ... | 2014 | 24962382 |
| in vitro genotoxic and mutagenic evaluation of the aqueous extract of codiaeum variegatum and its amoebicidal sub-fraction. | codiaeum variegatum, grown in many varieties, has been widely used as a houseplant based on its brightly decorative foliage. in addition, a variety of this plant has been used for a long time against bloody diarrhea by the local population in cameroon. | 2014 | 24971796 |
| caecal amoebic colitis mimicking a colorectal cancer. | intestinal entamoeba histolytica infection in the uk is rare but it can lead to colitis, abscess formation, colonic perforation and rarely amoeboma. although most patients are asymptomatic, it can present with a wide range of gastrointestinal symptoms depending on the phase of the infection. we present a case of amoebic colitis that caused diagnostic uncertainty by mimicking a colonic carcinoma. | 2011 | 24972391 |
| antiprotozoal activity of 8-acyl and 8-alkyl incomptine a analogs. | the activities of 11 c-8-o-acyl and alkyl analogs of the antiprotozoal sesquiterpene lactone, incomptine a (1) against entamoeba histolytica and giardia lamblia, were determined. here, the effects of different lengths and amounts of branching of the acyl and alkyl groups on the antiprotozoal activity of the synthesized incomptine a-analogs are reported. | 2014 | 24974343 |
| modified pehps medium as an alternative for the in vitro culture of giardia lamblia. | commercial culture media present interlot variations in biological activity. we have previously designed a homemade and economic culture medium, pehps medium, for the axenic cultivation of entamoeba histolytica and trichomonas vaginalis. trophozoites of amoebae and trichomonads grow well in this medium. furthermore, the medium is stable for several months when stored frozen or refrigerated. the objective of this work was to modify pehps medium to support the in vitro growth of giardia lamblia. i ... | 2014 | 24982905 |
| balantidiosis: a rare accidental finding in the urine of a patient with acute renal failure. | balantidium coli is the only ciliated protozoan which is known to infect human and nonhuman primates. route of infection is faecal-oral route. it is actively motile and causes mostly asymptomatic infections, or it may develop dysentery which is similar to that which is caused by entamoeba histolytica. here, we are describing a case of an accidental finding of b.coli in the urine of a patient who presented with acute renal failure, based on its characteristic morphology and motility which were se ... | 2014 | 24995185 |
| liver abscess in the tropics: an experience from jammu. | to study the various types of liver abscesses. this prospective study was conducted over a period of one year, from november 2011 to october 2012, at the department of general surgery in acharya shri chander college of medical sciences and hospital sidhra, jammu. | 2014 | 24996785 |
| proteomic analysis identifies endoribouclease ehl-psp and ehrrp41 exosome protein as novel interactors of ehcaf1 deadenylase. | in higher eukaryotic cells mrna degradation initiates by poly(a) tail shortening catalyzed by deadenylases caf1 and ccr4. in spite of the key role of mrna turnover in gene expression regulation, the underlying mechanisms remain poorly understood in parasites. here, we aimed to study the function of ehcaf1 and identify associated proteins in entamoeba histolytica. by biochemical assays, we evidenced that ehcaf1 has both rna binding and deadenylase activities in vitro. ehcaf1 was located in cytopl ... | 2014 | 24998979 |
| regulation of virulence of entamoeba histolytica. | entamoeba histolytica is the third-leading cause of parasitic mortality globally. e. histolytica infection generally does not cause symptoms, but the parasite has potent pathogenic potential. the origins, benefits, and triggers of amoebic virulence are complex. amoebic pathogenesis entails depletion of the host mucosal barrier, adherence to the colonic lumen, cytotoxicity, and invasion of the colonic epithelium. parasite damage results in colitis and, in some cases, disseminated disease. both ho ... | 2014 | 25002094 |
| erythrophagocytosis in entamoeba histolytica and entamoeba dispar: a comparative study. | entamoeba histolytica is the causative agent of human intestinal and liver amebiasis. the extraordinary phagocytic activity of e. histolytica trophozoites has been accepted as one of the virulence mechanisms responsible for their invasive capacity. the recognition of the noninvasive entamoeba dispar as a different species has raised the question as to whether the lack of pathogenic potential of this ameba correlates with a limited phagocytic capacity. we have therefore compared the process of er ... | 2014 | 25003123 |
| structural elucidation of a dual-activity pap phosphatase-1 from entamoeba histolytica capable of hydrolysing both 3'-phosphoadenosine 5'-phosphate and inositol 1,4-bisphosphate. | the enzyme 3'-phosphoadenosine 5'-phosphatase-1 (pap phosphatase-1) is a member of the li(+)-sensitive mg(2+)-dependent phosphatase superfamily, or inositol monophosphatase (impase) superfamily, and is an important regulator of the sulfate-activation pathway in all living organisms. inhibition of this enzyme leads to accumulation of the toxic byproduct 3'-phosphoadenosine 5'-phosphate (pap), which could be lethal to the organism. genomic analysis of entamoeba histolytica suggests the presence of ... | 2014 | 25004978 |
| crystallization and preliminary x-ray analysis of rabx3, a tandem gtpase from entamoeba histolytica. | ras superfamily gtpases regulate signalling pathways that control multiple biological processes by modulating the gtp/gdp cycle. various rab gtpases, which are the key regulators of vesicular trafficking pathways, play a vital role in the survival and virulence of the enteric parasite entamoeba histolytica. the rab gtpases act as binary molecular switches that utilize the conformational changes associated with the gtp/gdp cycle to elicit responses from target proteins and thereby regulate a broa ... | 2014 | 25005092 |
| amoebozoa possess lineage-specific globin gene repertoires gained by individual horizontal gene transfers. | the amoebozoa represent a clade of unicellular amoeboid organisms that display a wide variety of lifestyles, including free-living and parasitic species. for example, the social amoeba dictyostelium discoideum has the ability to aggregate into a multicellular fruiting body upon starvation, while the pathogenic amoeba entamoeba histolytica is a parasite of humans. globins are small heme proteins that are present in almost all extant organisms. although several genomes of amoebozoan species have b ... | 2014 | 25013378 |
| differential expression and immunolocalization of antioxidant enzymes in entamoeba histolytica isolates during metronidazole stress. | entamoeba histolytica infections are endemic in the indian subcontinent. five to eight percent of urban population residing under poor sanitary conditions suffers from entamoeba infections. metronidazole is the most widely prescribed drug used for amoebiasis. in order to understand the impact of metronidazole stress on the parasite, we evaluated the expression of two antioxidant enzymes, peroxiredoxin and fesod, in entamoeba histolytica isolates during metronidazole stress. the results reveal th ... | 2014 | 25013795 |
| entamoeba histolytica adaptation to glucose starvation: a matter of life and death. | parasites are often challenged by constant changes of the glucose concentration in their different hosts and/or within the different biotopes in the same host. during its life cycle, entamoeba histolytica, the causative protozoan parasite of human amoebiasis, is exposed to both a glucose-poor environment in the colon and a glucose-rich environment in the liver. high-throughput 'omics' technologies are now widely used to characterize the cell's global response to various stresses and these techno ... | 2014 | 25016247 |
| recent advances in molecular biology of parasitic viruses. | the numerous protozoa that can inhabit the human gastro-intestinal tract are known, yet little is understood of the viruses which infect these protozoa. the discovery, morphologic details, purification methods of virus-like particles, genome and proteome of the parasitic viruses, entamoeba histolytica, giardia lamblia, trichomonas vaginalis, and the eimeria sp. are described in this review. the protozoan viruses share many common features: most of them are rna or double-stranded rna viruses, ran ... | 2014 | 25019235 |
| caecal amebic colitis mimicking obstructing right sided colonic carcinoma with liver metastases: a rare case. | intestinal entamoeba histolytica infection can lead to colitis, abscess formation, colonic perforation and rarely amoeboma. we report a case of colonic amoebiasis, in which the presenting symptoms and radiological findings closely resembled an obstructing right-sided colonic carcinoma, with liver metastases. | 2016 | 25022419 |
| dna extraction from protozoan oocysts/cysts in feces for diagnostic pcr. | pcr detection of intestinal protozoa is often restrained by a poor dna recovery or by inhibitors present in feces. the need for an extraction protocol that can overcome these obstacles is therefore clear. qiaamp® dna stool mini kit (qiagen) was evaluated for its ability to recover dna from oocysts/cysts directly from feces. twenty-five giardia-positive, 15 cryptosporidium-positive, 15 entamoeba histolytica-positive, and 45 protozoa-free samples were processed as control by microscopy and immunoa ... | 2014 | 25031466 |
| identifying etiological agents causing diarrhea in low income ecuadorian communities. | continued success in decreasing diarrheal disease burden requires targeted interventions. to develop such interventions, it is crucial to understand which pathogens cause diarrhea. using a case-control design we tested stool samples, collected in both rural and urban ecuador, for 15 pathogenic microorganisms. pathogens were present in 51% of case and 27% of control samples from the urban community, and 62% of case and 18% of control samples collected from the rural community. rotavirus and shige ... | 2014 | 25048373 |
| asymptomatic intestinal amebiasis in japanese hiv-1-infected individuals. | seventy-one asymptomatic human immunodeficiency virus-1 (hiv-1) -infected individuals who underwent colonoscopy for detection of diseases other than amebiasis were included in this study. ulcerative lesions caused by entamoeba histolytica were identified by colonoscopy and biopsy in 11.3% (8 of 71) of individuals. stool microscopic examination hardly identified entamoeba, whereas serum antibody against e. histolytica was often elevated in patients with subclinical intestinal amebiasis. human leu ... | 2014 | 25048374 |
| repurposing the open access malaria box to discover potent inhibitors of toxoplasma gondii and entamoeba histolytica. | toxoplasmosis and amebiasis are important public health concerns worldwide. the drugs currently available to control these diseases have proven limitations. therefore, innovative approaches should be adopted to identify and develop new leads from novel scaffolds exhibiting novel modes of action. in this paper, we describe results from the screening of compounds in the medicines for malaria venture (mmv) open access malaria box in a search for new anti-toxoplasma and anti-entamoeba agents. standa ... | 2014 | 25049259 |
| amoebic anal fistula: new insight into an old disease. | a 67-year-old gentleman underwent fistulectomy for low trans-sphincteric anal fistula along with curettage for an associated abscess extending proximally for half a centimeter into the intersphincteric plane. the roof of the cavity became clearly visible after satisfactory culmination of the surgical procedure. histopathological examination of the fistulous tract and the curetted granulation tissue revealed presence of multiple trophozoites of entamoeba histolytica exhibiting erythrophagocytosis ... | 2014 | 25053686 |
| hypocholesterolemia in patients with an amebic liver abscess. | many parasites induce changes in the lipid profiles of the host. cholesterol increases the virulence of entamoeba histolytica in animal models and in vitro culture. this study aimed to determine, in patients with an amebic liver abscess, the correlation between cholesterol and other features, such as the size and number of abscesses, standard hematological and serum chemistry profiles, liver tests, and duration of hospital stay. | 2014 | 25071907 |
| regulation of gene expression in the protozoan parasite entamoeba invadens: identification of core promoter elements and promoters with stage-specific expression patterns. | developmental switching between life-cycle stages is a common feature among many pathogenic organisms. entamoeba histolytica is an important human pathogen and is a leading parasitic cause of death globally. during its life cycle, entamoeba converts between cysts (essential for disease transmission) and trophozoites (responsible for tissue invasion). despite being central to its biology, the triggers that are involved in the developmental pathways of this parasite are not well understood. in ord ... | 2014 | 25075445 |
| serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. | a strategy to combat infectious diseases, including neglected tropical diseases (ntds), will depend on the development of reliable epidemiological surveillance methods. to establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in kenya. | 2014 | 25078404 |
| noninvasive intestinal amebiasis: entamoeba histolytica colonization without invasion. | entamoeba histolytica infection remains a major cause of morbidity and mortality worldwide. although research with the organism began in the late nineteenth century, our understanding of the natural history of the infection remains incomplete and is heavily based on expert opinion. most persons infected with e. histolytica are carriers with the organism colonizing the large intestine. host defense mechanisms that prevent invasive diseases are poorly understood. a timely review could lead to rene ... | 2014 | 25101557 |
| cellular and proteomics analysis of the endomembrane system from the unicellular entamoeba histolytica. | entamoeba histolytica is the protozoan parasite agent of amoebiasis, an infectious disease of the human intestine and liver. specific active pathogenic factors are secreted toward the external milieu upon interaction of the parasite with human tissue. trafficking dynamics and secretion of these factors is not known and characterization of the dynamics interplay of subcellular compartments such as the er or golgi apparatus is still pending. in this work, we took advantage of cell fractionation an ... | 2015 | 25109464 |
| proteomic analysis of entamoeba histolytica in vivo assembled pre-mrna splicing complexes. | the genome of the human intestinal parasite entamoeba histolytica contains nearly 3000 introns and bioinformatic predictions indicate that major and minor spliceosomes occur in entamoeba. however, except for the u2-, u4-, u5- and u6 snrnas, no other splicing factor has been cloned and characterized. here, we ha-tagged cloned the snrnp component u1a and assessed its expression and nuclear localization. because the snrnp-free u1a form interacts with polyadenylate-binding protein, ha-u1a immunoprec ... | 2014 | 25109466 |
| discrimination of entamoeba spp. in children with dysentery. | the present study was performed in order to differentiate e. histolytica and e. dispar in children from gorgan city, using a pcr method. | 2014 | 25120897 |
| prevalence of intestinal parasitic infections among communities living in different habitats and its comparison with one hundred and one studies conducted over the past 42 years (1970 to 2013) in malaysia. | intestinal parasitic infections are among the most common diseases affecting mankind causing major public health problems to billions of people living in developing countries. the aim of this study is to determine the prevalence of intestinal parasites in various communities residing in different habitats in malaysia and compare the findings with 101 studies conducted over the past 42 years (1970-2013). a cross-sectional study design was conducted with the aid of a questionnaire to collect relev ... | 2014 | 25134888 |
| genetic variability of the serine-rich entamoeba histolytica protein gene in clinical isolates from the united arab emirates. | the genetic diversity of 20 entamoeba histolytica isolates from asymptomatic individuals from the uae was investigated by analyzing polymorphism in the serine-rich e. histolytica gene (srehp) by nested polymerase chain reaction (pcr) amplification followed by restriction fragment length polymorphism (rflp) on dna extracted directly from stool samples. the srehp gene was successfully amplified in 15 out of 20 e. histolytica-positive samples. four out of the remaining five isolates did not amplify ... | 2014 | 25134907 |
| different profile of intestinal protozoa and helminthic infections among patients with diarrhoea according to age attending a rural hospital in southern ethiopia. | the aim of this study was to analyze the association of intestinal parasitic diseases with age and gender in patients with diarrhea attending a rural hospital in southern ethiopia in the period 2007-2012. a total of 32,191 stool examination was performed in patients who presented with diarrhea. the overall prevalence of intestinal parasites in the present study was 26.5%. predominant parasites detected were giardia lamblia (15.0%), entamoeba histolytica/dispar (5.4%), and ascaris lumbricoides (5 ... | 2014 | 25134911 |
| genetic analysis of enterobius vermicularis isolated from a chimpanzee with lethal hemorrhagic colitis and pathology of the associated lesions. | human pinworms, enterobius vermicularis, are normally recognized as minor pathogens. however, a fatal case of human pinworm infection has been reported in a nonhuman primate, a zoo reared chimpanzee. here, we histopathologically examined the lesions in tissues from the deceased chimpanzee and genetically characterized the isolated worms to investigate the pathogenicity and determine the phylogeny. we identified ulcers deep in the submucosa where many parasites were found to have invaded the lami ... | 2014 | 25138069 |
| is routine medical examination of food handlers enough to ensure food safety in hospitals? | nosocomial food outbreaks due to infected food handlers is primarily due to inadequate knowledge and faulty practices of food handlers during diarrhoeal episodes. the aim of this study was to assess: 1) prevalence of enteropathogen infection among food handlers working in our hospital during 2007 to 2011 and 2) adequacy of precautions taken by them during gastroenteritis episodes. stool samples submitted by food handlers during 2007 to 2011 were examined for the presence of enteropathogens by st ... | 2012 | 25145060 |
| in vitro antiamoebic activity evaluation and docking studies of metronidazole-triazole hybrids. | an in-house database of 520 compounds was docked against entamoeba histolytica thioredoxin reductase (ehtrr), a promising target for the treatment of amoebiasis. amongst these, some metronidazole (mtz)-triazole hybrids were ranked high, with docking scores from -10.23 to -7.56. studies of the binding orientations and conformations show that the head groups of mtz-triazole hybrids interact with the arginine residues within the binding pocket of ehtrr, making it clear that such is the optimal and ... | 2014 | 25146853 |
| flavodiiron oxygen reductase from entamoeba histolytica: modulation of substrate preference by tyrosine 271 and lysine 53. | flavodiiron proteins (fdps) are a family of enzymes endowed with bona fide oxygen- and/or nitric-oxide reductase activity, although their substrate specificity determinants remain elusive. after a comprehensive comparison of available three-dimensional structures, particularly of fdps with a clear preference toward either o2 or no, two main differences were identified near the diiron active site, which led to the construction of site-directed mutants of tyr(271) and lys(53) in the oxygen reducin ... | 2014 | 25151360 |
| an uncommon case of hepatopulmonary amoebiasis. | amoebiasis is an uncommon infection in developed countries caused by the protozoan entamoeba histolytica. amoebic liver abscess is the most frequent extraintestinal presentation of the disease; pleuropulmonary involvement is rare, occurring mostly by rupture of the abscess into the pleural space or lung parenchyma. we describe a case of a 48-year-old migrant from são tomé e príncipe, with fever, wasting, dry cough and right upper abdominal pain for the past 2 months. the ct scan revealed a volum ... | 2014 | 25155487 |
| invasive amoebiasis: a review of entamoeba infections highlighted with case reports. | entamoeba histolytica infections of the gastrointestinal tract are common in the developing world but rare in north america. the authors present two cases: one involving an individual who had not travelled to an endemic area and another involving an individual who was born in bulgaria. both presented with severe abdominal pain and diarrhea. endoscopic assessment revealed scattered colonic ulcerations and one patient was found to have a liver abscess on imaging. stool ova and parasite studies wer ... | 2016 | 25157525 |
| beta carbonic anhydrases: novel targets for pesticides and anti-parasitic agents in agriculture and livestock husbandry. | the genomes of many insect and parasite species contain beta carbonic anhydrase (β-ca) protein coding sequences. the lack of β-ca proteins in mammals makes them interesting target proteins for inhibition in treatment of some infectious diseases and pests. many insects and parasites represent important pests for agriculture and cause enormous economic damage worldwide. meanwhile, pollution of the environment by old pesticides, emergence of strains resistant to them, and their off-target effects a ... | 2014 | 25174433 |
| entamoeba histolytica acetyl-coa synthetase: biomarker of acute amoebic liver abscess. | to characterize the entamoeba histolytica (e. histolytica) antigen(s) recognized by moribound amoebic liver abscess hamsters. | 2014 | 25182945 |
| prevalence of entamoeba histolytica -like cysts compared to e. histolytica antigens detected by elisa in the stools of 600 patients from three socioeconomic communities in the metropolitan city of lahore, pakistan. | amoebiasis, caused by entamoeba histolytica , has a worldwide distribution and is of public health significance in many developing countries. it has a fecal-oral transmission cycle and is most prevalent in developing countries in regions where substandard sanitary conditions exist due to poverty. little is known about the epidemiology of e. histolytica infection and its presence in different socioeconomic communities in developing countries. we undertook the present study in the city of lahore, ... | 2015 | 25189631 |
| duplex microfluidic sers detection of pathogen antigens with nanoyeast single-chain variable fragments. | quantitative and accurate detection of multiple biomarkers would allow for the rapid diagnosis and treatment of diseases induced by pathogens. monoclonal antibodies are standard affinity reagents applied for biomarkers detection; however, their production is expensive and labor-intensive. herein, we report on newly developed nanoyeast single-chain variable fragments (nyscfv) as an attractive alternative to monoclonal antibodies, which offers the unique advantage of a cost-effective production, s ... | 2014 | 25192256 |
| [ameboma: possible therapeutic decisions in an amebiasis-endemic region]. | amebiasis can mimic cecal tumors. unless this infection is diagnosed in a timely manner, affected individuals may undergo extensive surgery. | 2015 | 25195079 |
| rnai pathway genes are resistant to small rna mediated gene silencing in the protozoan parasite entamoeba histolytica. | the rna interference pathway in the protist entamoeba histolytica plays important roles in permanent gene silencing as well as in the regulation of virulence determinants. recently, a novel rna interference (rnai)-based silencing technique was developed in this parasite that uses a gene endogenously silenced by small rnas as a "trigger" to induce silencing of other genes that are fused to it. fusion to a trigger gene induces the production of gene-specific antisense small rnas, resulting in robu ... | 2014 | 25198343 |
| immunity in urogenital protozoa. | innate and adaptive immunity play a significant role in urogenital infections. innate immunity is provided by the epithelial cells and mucus lining along with acidic ph, which forms a strong physical barrier against the pathogens in female reproductive tract. cells of innate immune system, antimicrobial peptides, cytokines, chemokines and adaptive immunity in the reproductive tract are evolved during infection, and a pro-inflammatory response is generated to fight against the invading pathogen t ... | 2014 | 25201404 |
| ehcoactosin stabilizes actin filaments in the protist parasite entamoeba histolytica. | entamoeba histolytica is a protist parasite that is the causative agent of amoebiasis, and is a highly motile organism. the motility is essential for its survival and pathogenesis, and a dynamic actin cytoskeleton is required for this process. ehcoactosin, an actin-binding protein of the adf/cofilin family, participates in actin dynamics, and here we report our studies of this protein using both structural and functional approaches. the x-ray crystal structure of ehcoactosin resembles that of hu ... | 2014 | 25210743 |
| gene expression profiling in entamoeba histolytica identifies key components in iron uptake and metabolism. | entamoeba histolytica is an ameboid parasite that causes colonic dysentery and liver abscesses in humans. the parasite encounters dramatic changes in iron concentration during its invasion of the host, with relatively low levels in the intestinal lumen and then relatively high levels in the blood and liver. the liver notably contains sources of iron; therefore, the parasite's ability to use these sources might be relevant to its survival in the liver and thus the pathogenesis of liver abscesses. ... | 2014 | 25210888 |
| a new human 3d-liver model unravels the role of galectins in liver infection by the parasite entamoeba histolytica. | investigations of human parasitic diseases depend on the availability of appropriate in vivo animal models and ex vivo experimental systems, and are particularly difficult for pathogens whose exclusive natural hosts are humans, such as entamoeba histolytica, the protozoan parasite responsible for amoebiasis. this common infectious human disease affects the intestine and liver. in the liver sinusoids e. histolytica crosses the endothelium and penetrates into the parenchyma, with the concomitant i ... | 2014 | 25211477 |
| identification of phosphatidylcholine transfer protein-like in the parasite entamoeba histolytica. | caveolin is the protein marker of caveola-mediated endocytosis. previously, we demonstrated by immunoblotting and immunofluorescence that an anti-chick embryo caveolin-1 monoclonal antibody (mab) recognizes a protein in amoeba extracts. nevertheless, the caveolin-1 gene is absent in the entamoeba histolytica genome database. in this work, the goal was to isolate, identify and characterize the protein that cross-reacts with chick embryo caveolin-1. we identified the protein using a proteomic appr ... | 2014 | 25223890 |