Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| punica granatum leaf ethanolic extract and ellagic acid as inhibitors of zika virus infection. | zika virus, an arthropod-borne flavivirus, is an emerging healthcare threat worldwide. zika virus is responsible for severe neurological effects, such as paralytic guillain-barrè syndrome, in adults, and also congenital malformations, especially microcephaly. no specific antiviral drugs and vaccines are currently available, and treatments are palliative, but medicinal plants show great potential as natural sources of anti-zika phytochemicals. this study deals with the investigation of the compos ... | 2020 | 32937663 |
| the molecular interactions of zikv and denv with the type-i ifn response. | zika virus (zikv) and dengue virus (denv) are related viruses of the flavivirus genus that cause significant disease in humans. existing control measures have been ineffective at curbing the increasing global incidence of infection for both viruses and they are therefore prime targets for new vaccination strategies. type-i interferon (ifn) responses are important in clearing viral infection and for generating efficient adaptive immune responses towards infection and vaccination. however, zikv an ... | 2020 | 32937990 |
| diagnostic approach for arboviral infections in the united states. | domestic arthropod-borne viruses (arboviruses) are single stranded rna viruses, the most common of which include the mosquito-borne west nile virus, st. louis encephalitis virus, la crosse virus, jamestown canyon virus, and eastern equine encephalitis virus, as well as the tick-borne powassan virus. previously considered to be rare infections, they have been detected with increasing frequency over the past 2 decades. here, we present an overview of the domestic arboviruses listed above and descr ... | 2020 | 32938736 |
| salivary extracellular vesicles inhibit zika virus but not sars-cov-2 infection. | zika virus (zikv) is mainly transmitted via mosquitos, but human-to-human transmissions also occur. the virus is shed into various body fluids including saliva, which represents a possible source of viral transmission. thus, we here explored whether human saliva affects zikv infectivity. we found that physiological concentrations of pooled saliva dose-dependently inhibit zikv infection of monkey and human cells by preventing viral attachment to target cells. the anti-zikv activity in saliva coul ... | 2020 | 32939236 |
| obstetric and perinatal outcomes in cases of congenital zika syndrome. | to describe obstetric and perinatal outcomes in cases of congenital zika syndrome (czs). | 2020 | 32939752 |
| characterization of zika virus infection of human fetal cardiac mesenchymal stromal cells. | zika virus (zikv) is a single-stranded rna virus belonging to the family flaviviridae. zikv predominantly enters cells using the tam-family protein tyrosine kinase receptor axl, which is expressed on a range of cell types, including neural progenitor cells, keratinocytes, dendritic cells, and osteoblasts. zikv infections have been associated with fetal brain damage, which prompted the world health organization to declare a public health emergency in 2016. zikv infection has also been linked to b ... | 2020 | 32941515 |
| clinical and laboratory findings of acute zika virus infection in patients from salvador during the first brazilian epidemic. | several major epidemics of zika fever, caused by the zika virus (zikv), have emerged in brazil since early 2015, eventually spreading to other countries on the south american continent. the present study describes the clinical manifestations and laboratory findings of patients with confirmed acute zikv infection during the first epidemic that occurred in salvador, brazil. all included patients were seen at the emergency room of a private tertiary hospital located in salvador, brazil from 2015 th ... | 2020 | 32941805 |
| ivermectin as a broad-spectrum host-directed antiviral: the real deal? | the small molecule macrocyclic lactone ivermectin, approved by the us food and drug administration for parasitic infections, has received renewed attention in the last eight years due to its apparent exciting potential as an antiviral. it was identified in a high-throughput chemical screen as inhibiting recognition of the nuclear localizing human immunodeficiency virus-1 (hiv-1) integrase protein by the host heterodimeric importin (imp) α/β1 complex, and has since been shown to bind directly to ... | 2020 | 32942671 |
| disulfide reduction allosterically destabilizes the β-ladder subdomain assembly within the ns1 dimer of zikv. | the zika virus (zikv) was responsible for a recent debilitating epidemic that till date has no cure. a potential way to reduce zikv virulence is to limit the action of the nonstructural proteins involved in its viral replication. one such protein, ns1, encoded as a monomer by the viral genome, plays a major role via symmetric oligomerization. we examine the homodimeric structure of the dominant β-ladder segment of ns1 with extensive all atom molecular dynamics. we find it stably bounded by two s ... | 2020 | 32946768 |
| assessing the role of two populations of aedes japonicus japonicus for zika virus transmission under a constant and a fluctuating temperature regime. | since the huge epidemic of zika virus (zikv) in brazil in 2015, questions were raised to understand which mosquito species could transmit the virus. aedes aegypti has been described as the main vector. however, other aedes species (e.g. ae. albopictus and ae. japonicus) proven to be competent for other flaviviruses (e.g. west nile, dengue and yellow fever), have been described as potential vectors for zikv under laboratory conditions. one of these, the asian bush mosquito, ae. japonicus, is wide ... | 2020 | 32948231 |
| pyriproxyfen does not cause microcephaly or malformations in a preclinical mammalian model. | pyriproxyfen is used in brazil to combat epidemics of dengue fever, chikungunya fever, and zika virus. this study assessed the effects of pyriproxyfen on reproductive performance, embryo-fetal development, head measurements, and dna integrity in a preclinical model. thirty pregnant mice were divided into three groups (n = 10): control (drinking water-0.1 ml/10 g (body weight-b.w., gavage) and treated with pyriproxyfen 0.0002 mg/kg and 0.0021 mg/kg (b.w., gavage) during the gestational period. an ... | 2020 | 32948939 |
| castanospermine reduces zika virus infection-associated seizure by inhibiting both the viral load and inflammation in mouse models. | zika virus (zikv) outbreaks have been reported worldwide, including a recent occurrence in brazil where it spread rapidly, and an association with increased cases of microcephaly was observed in addition to neurological issues such as gbs that were reported during previous outbreaks. following infection of neuronal tissues, zikv can cause inflammation, which may lead to neuronal abnormalities, including seizures and paralysis. therefore, a drug containing both anti-viral and immunosuppressive pr ... | 2020 | 32949636 |
| dengue and zika virus infections are enhanced by live attenuated dengue vaccine but not by recombinant dsv4 vaccine candidate in mouse models. | a tetravalent live attenuated dengue vaccine, dengvaxia, sensitised naïve recipients to severe dengue illness upon a subsequent natural dengue infection and is suspected to be due to antibody-dependent enhancement (ade). ade has also been implicated in the severe neurological outcomes of zika virus (zikv) infection. it has become evident that cross-reactive antibodies targeting the viral pre-membrane protein and fusion-loop epitope are ade-competent. a pre-clinical tetravalent dengue sub-unit va ... | 2020 | 32949997 |
| risk of zika virus-associated birth defects in congenital confirmed cases in the brazilian amazon. | to establish the risk of microcephaly in neonates born to women infected with zikv during pregnancy. | 2020 | 32952536 |
| epidemiologic and spatiotemporal trends of zika virus disease during the 2016 epidemic in puerto rico. | after zika virus (zikv) emerged in the americas, laboratory-based surveillance for arboviral diseases in puerto rico was adapted to include zikv disease. | 2020 | 32956416 |
| surveillance of aedes aegypti populations in the city of praia, cape verde: zika virus infection, insecticide resistance and genetic diversity. | aedes spp. are responsible for the transmission of many arboviruses, which contribute to rising human morbidity and mortality worldwide. the aedes aegypti mosquito is a main vector for chikungunya, dengue and yellow fever infections, whose incidence have been increasing and distribution expanding. this vector has also driven the emergence of the zika virus (zikv), first reported in africa which spread rapidly to asia and more recently across the americas. during the outbreak in the americas, cap ... | 2020 | 32958043 |
| maternal infections. | congenital infections are infections transmitted from mother to child during pregnancy (transplacentally) or delivery (peripartum). they have the potential to adversely affect fetal development and long-term neurodevelopmental outcome through inflammatory, destructive, developmental, or teratogenic lesions of the brain. because the fetal/neonatal brain has a limited capacity to respond to injury, early inflammatory changes may be difficult to visualize and only manifest as neurocognitive disabil ... | 2020 | 32958187 |
| production of zika virus virus-like particles. | zika virus (zikv) is a mosquito-transmitted virus that has caused major outbreaks of disease around the world over the last few years. the infectious zikv consists of a structural protein outer shell surrounding a nucleocapsid. virus-like particles (vlp) consist of the outer structural protein shell, but without the nucleocapsid, and are hence noninfectious. vlp, however, are structurally equivalent to the native virus and thus present a similar antigenic profile. these properties make them good ... | 2021 | 32959245 |
| structural determinants of trpv4 inhibition and identification of new antagonists with antiviral activity. | the transient receptor potential vanilloid 4 (trpv4) cation channel participates in multiple physiological processes and is also at the core of different diseases, making this channel an interesting pharmacological target with therapeutic potential. however, little is known about the structural elements governing its inhibition. | 2020 | 32959389 |
| identification of inhibitors of zikv replication. | zika virus (zikv) was identified in 1947 in the zika forest of uganda and it has emerged recently as a global health threat, with recurring outbreaks and its associations with congenital microcephaly through maternal fetal transmission and guillain-barré syndrome. currently, there are no united states (us) food and drug administration (fda)-approved vaccines or antivirals to treat zikv infections, which underscores an urgent medical need for the development of disease intervention strategies to ... | 2020 | 32961956 |
| zika virus seroprevalence in women who gave birth during zika virus outbreak in brazil - a prospective observational study. | the recent zika virus (zikv) outbreak in brazil started in august 2015 and ended in may 2017 without effective public health measures for its control have been taken. the immunological status of a community may not only predict future outbreaks as well to answer questions regarding zikv not known yet. | 2020 | 32964154 |
| clinical, virological, and immunological profiles of denv, zikv, and/or chikv-infected brazilian patients. | arboviruses co-circulating within a population that are transmitted by the same vector have the potential to cause coinfections. coinfections with dengue virus (denv), zika virus (zikv), and chikungunya virus (chikv) have been occurring in brazil, but it is not well-understood how human responses vary during mono- or coinfections and whether they play different roles in pathogenesis. | 2020 | 32966990 |
| inositol-requiring enzyme 1α promotes zika virus infection through regulation of stearoyl coenzyme a desaturase 1-mediated lipid metabolism. | zika virus (zikv) is an emerging mosquito-borne flavivirus which has become a global epidemic threat due to its rapid spread and association with serious consequences of infection, including neonatal microcephaly. inositol-requiring enzyme 1α (ire1α) is an endoplasmic reticulum (er)-related transmembrane protein that mediates unfolded protein response (upr) pathway and has been indicated to play an important role in flavivirus replication. however, the mechanism of how ire1α affects zikv replica ... | 2020 | 32967957 |
| azithromycin in viral infections. | azithromycin (azm) is a synthetic macrolide antibiotic effective against a broad range of bacterial and mycobacterial infections. due to an additional range of anti-viral and anti-inflammatory properties, it has been given to patients with the coronaviruses sars-cov or mers-cov. it is now being investigated as a potential candidate treatment for sars-cov-2 having been identified as a candidate therapeutic for this virus by both in vitro and in silico drug screens. to date there are no randomised ... | 2020 | 32969125 |
| the allocation of usdollar;105 billion in global funding from g20 countries for infectious disease research between 2000 and 2017: a content analysis of investments. | each year, billions of us$ are spent globally on infectious disease research and development. however, there is little systematic tracking of global research and development. we present research on investments into infectious diseases research from funders in the g20 countries across an 18-year time period spanning 2000-17, comparing amounts invested for different conditions and considering the global burden of disease to identify potential areas of relative underfunding. | 2020 | 32971052 |
| evaluation of immunoglobulin m-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a national reference laboratory. | zika and dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. due to brazil's wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by national reference laboratory for arboviruses in brazil and evaluate commercial tests available. we evaluated in house igm elisas for the individual detection of anti-zikv, -denv, and -yfv igm, against a panel of ... | 2020 | 32971183 |
| a screening study of high affinity peptide as molecular binder for axl, tyrosine kinase receptor involving in zika virus entry. | the recent extensive spread of zika virus has led to increased interest in the development of early diagnostic tests. to the best of our knowledge, this is the first study to demonstrate the successful use of phage display to identify affinity peptides for quantitative analysis of axl, a tyrosine kinase receptor involved in zika virus entry. biopanning of m13 phage library successfully identified a high affinity peptide, with the sequence ahnhtpikqkyl. to study the feasibility of using free pept ... | 2020 | 32971483 |
| field-deployable molecular diagnostic platform for arbovirus detection in aedes aegypti. | surveillance of mosquito infection status is critical for planning and deployment of proper mosquito control initiatives. point-of-care (poc) detection assays are necessary for monitoring the infection prevalence and geographical range of viruses in mosquito vector populations. we therefore assessed the novel real-time pcr (qpcr) bcube (hyris, london, uk) molecular diagnostic system as a tool for virus detection. | 2020 | 32972453 |
| effector cd8 t cell-dependent zika virus control in the cns: a matter of time and numbers. | zika virus (zikv), a mosquito-borne flavivirus, came into the spotlight in 2016 when it was found to be associated with an increased rate of microcephalic newborns in brazil. the virus has further been recognized to cause neurologic complications in children and adults in the form of myelitis, encephalitis, acute disseminated encephalomyelitis (adem) and guillain barre syndrome in a fraction of infected individuals. with the ultimate goal of identifying correlates of protection to guide the desi ... | 2020 | 32973802 |
| dengue and zika viruses: epidemiological history, potential therapies, and promising vaccines. | dengue virus (denv), which can lead to fatal hemorrhagic fever, affects 390 million people worldwide. the closely related zika virus (zikv) causes microcephaly in newborns and guillain-barré syndrome in adults. both viruses are mostly transmitted by aedes albopictus and aedes aegypti mosquitoes, which, due to globalization of trade and travel alongside climate change, are spreading worldwide, paving the way to denv and zikv transmission and the occurrence of new epidemics. local outbreaks have a ... | 2020 | 32977703 |
| programmable low-cost dna-based platform for viral rna detection. | detection of viruses is critical for controlling disease spread. recent emerging viral threats, including zika virus, ebola virus, and sars-cov-2 responsible for coronavirus disease 2019 (covid-19) highlight the cost and difficulty in responding rapidly. to address these challenges, we develop a platform for low-cost and rapid detection of viral rna with dna nanoswitches that mechanically reconfigure in response to specific viruses. using zika virus as a model system, we show nonenzymatic detect ... | 2020 | 32978154 |
| avian anti-ns1 igy antibodies neutralize dengue virus infection and protect against lethal dengue virus challenge. | dengue is the most prevalent arboviral disease in humans and a continually increasing global public health burden. to date, there are no approved antiviral therapies against dengue virus (denv) and the only licensed vaccine, dengvaxia, is exclusively indicated for individuals with prior denv infection. endothelial hyperpermeability and vascular leak, pathogenic hallmarks of severe dengue disease, can be directly triggered by denv non-structural protein 1 (ns1). as such, anti-ns1 antibodies can p ... | 2020 | 32979401 |
| protective and enhancing interactions among dengue viruses 1-4 and zika virus. | dengue viruses 1-4 (denv 1-4) and zika virus (zikv) are closely related flaviviruses transmitted by aedes mosquitoes that co-circulate in asia, the americas, africa, and oceania. here, we review recent and historical literature on in vitro experiments, animal models, and clinical and epidemiological studies to describe how the sequence of denv 1-4 and zikv infections modulates subsequent dengue and zika disease outcome. overall, we find these interactions are asymmetric. immunity from a prior de ... | 2020 | 32979816 |
| identifying optimal capsid duplication length for the stability of reporter flaviviruses. | abstract mosquito-transmitted flaviviruses cause widespread disease across the world. to provide better molecular tools for drug screens and pathogenesis studies, we report a new approach to produce stable nanoluc-tagged flaviviruses, including dengue virus serotypes 1-4, japanese encephalitis virus, yellow fever virus, west nile virus, and zika virus. since the reporter gene is often engineered at the capsid gene region, the capsid sequence must be duplicated to flank the reporter gene; such ca ... | 2020 | 32981479 |
| zika induces human placental damage and inflammation. | in brazil, an epidemic of zika virus (zikv) infections was declared in 2015 that coincided with alarming reports of microcephaly in newborns associated with mother infection. although the virus has placental tropism, changes in the tissue morphology and immunity of infected patients have not yet been elucidated. here, we investigated the histopathological and ultrastructural changes along with the immunological profile and the bdnf expression in rare placental material. tissues were obtained in ... | 2020 | 32983175 |
| long-term outcomes of infants with congenital zika virus infection in ecuador: a retrospective longitudinal study. | few congenital zika syndrome (czs) cases have been notified in ecuador and, to our knowledge, there are no significant published studies dealing with their clinical evolution. we present a detailed clinical characterization of 21 children with congenital zika virus (zikv) infection born in ecuador who were followed up until september 2019. | 2020 | 32984903 |
| surveillance of invasive aedes mosquitoes along swiss traffic axes reveals different dispersal modes for aedes albopictus and ae. japonicus. | over the past three decades, europe has witnessed an increased spread of invasive aedine mosquito species, most notably aedes albopictus, a key vector of chikungunya, dengue and zika virus. while its distribution in southern europe is well documented, its dispersal modes across the alps remain poorly investigated, preventing a projection of future scenarios beyond its current range in order to target mosquito control. to monitor the presence and frequency of invasive aedes mosquitoes across and ... | 2020 | 32986704 |
| downregulation of igf2 expression in third trimester placental tissues from zika virus infected women in brazil. | screening for genes differentially expressed in placental tissues, aiming to identify transcriptional signatures that may be involved in zikv congenital pathogenesis. | 2020 | 32987099 |
| age and geographic dependence of zika virus infection during the outbreak on yap island, 2007. | intensive surveillance of zika virus infection conducted on yap island has provided crucial information on the epidemiological characteristics of the virus, but the rate of infection and medical attendance stratified by age and geographical location of the epidemic have yet to be fully clarified. in the present study, we reanalyzed surveillance data reported in a previous study. likelihood-based bayesian inference was used to gauge the age and geographically dependent force of infection and age- ... | 2020 | 32987571 |
| perceptions and emotional state of mothers of children with and without microcephaly after the zika virus epidemic in rural caribbean colombia. | zika virus (zikv) infection during pregnancy can cause neurological manifestations such as microcephaly. the aim of this study was to explore perceptions of zikv and mental health in women exposed to zikv during pregnancy in colombia. this was a mixed-methods study based on structured interviews and psychological tests. structured interviews were transcribed and analysed with atlas ti software. a grounded theory approach was applied. quantitative analysis was performed with statistical package f ... | 2020 | 32992703 |
| neurological development, epilepsy, and the pharmacotherapy approach in children with congenital zika syndrome: results from a two-year follow-up study. | clinical outcomes related to congenital zika syndrome (czs) include microcephaly accompanied by specific brain injuries. among several czs outcomes that have been described, epilepsy and motor impairments are present in most cases. pharmacological treatment for seizures resulting from epilepsy is performed with anticonvulsant drugs, which in the long term are related to impairments in the child's neuropsychomotor development. here, we describe the results from a two-year follow-up of a cohort of ... | 2020 | 32992985 |
| a new subclass of exoribonuclease-resistant rna found in multiple genera of flaviviridae. | viruses have developed innovative strategies to exploit the cellular machinery and overcome the antiviral defenses of the host, often using specifically structured rna elements. examples are found in the flavivirus genus (in the family flaviviridae), where during flaviviral infection, pathogenic subgenomic flaviviral rnas (sfrnas) accumulate in the cell. these sfrnas are formed when a host cell 5' to 3' exoribonuclease degrades the viral genomic rna but is blocked by an exoribonuclease-resistant ... | 2020 | 32994331 |
| facing a new reality from the zika virus congenital syndrome: the families' perspective. | this paper presents the partial results of the research on the social implications of addressing the zika virus congenital syndrome (zvcs). this study aimed to analyze the repercussions of the diagnosis on the life of the families attended at an institute of reference in rio de janeiro, based on a qualitative study built on the thematic analysis of the data collected through semi-structured interviews with 15 mothers from october 2017 to june 2018. the results revealed the helplessness of famili ... | 2020 | 32997012 |
| dengue and zika virus multi-epitope antigen expression in insect cells. | dengue virus and zika virus are arthropod-borne flaviviruses that cause millions of infections worldwide. the co-circulation of both viruses makes serological diagnosis difficult as they share high amino acid similarities in viral proteins. antigens are one of the key reagents in the differential diagnosis of these viruses through the detection of igg antibodies in serological assays during the convalescent-phase of infections. here, we report the expression of dengue virus (denv) and zika virus ... | 2020 | 32997310 |
| potential for virus endogenization in humans through testicular germ cell infection: the case of hiv. | viruses have colonized the germ line of our ancestors at several occasions during evolution, leading to the integration in the human genome of viral sequences from over 30 retroviral groups and a few non-retroviruses. among the recently emerged viruses infecting humans, several target the testis (eg hiv, zika and ebola viruses). here we aimed to investigate whether human testicular germ cells (tgcs) can support integration by hiv, a contemporary retrovirus that started to spread in the human pop ... | 2020 | 32999017 |
| two sides of a coin: a zika virus mutation selected in pregnant rhesus macaques promotes fetal infection in mice but at a cost of reduced fitness in nonpregnant macaques and diminished transmissibility by vectors. | although fetal death is now understood to be a severe outcome of congenital zika syndrome, the role of viral genetics is still unclear. we sequenced zika virus (zikv) from a rhesus macaque fetus that died after inoculation and identified a single intra-host substitution, m1404i, in the zikv polyprotein, located in ns2b. targeted sequencing flanking position 1404 in 9 additional macaque mothers and their fetuses identified m1404i at sub-consensus frequency in the majority (5 of 9, 56%) of animals ... | 2020 | 32999034 |
| zika virus infection leads to variable defects in multiple neurological functions and behaviors in mice and children. | zika virus (zikv) has evolved into a global health threat because of its causal link to congenital zika syndrome. zikv infection of pregnant women may cause a spectrum of abnormalities in children. in the studies in brazil, a large cohort of children with perinatal exposure to zikv is followed, and a spectrum of neurodevelopmental abnormalities is identified. in parallel, it is demonstrated that infection of the mouse neonatal brain by a contemporary zikv strain instead of an asian ancestral str ... | 2020 | 32999822 |
| establishment and characterization of a cell line from ghanaian aedes aegypti (diptera: culicidae) focusing on aedes-borne flavivirus susceptibility. | mosquitoes are generally considered one of the most important vectors of arboviruses, with aedes aegypti regarded as the most important in transmission of yellow fever and dengue viruses. to investigate why there are differences in the incidence of dengue fever and zika in different geographical areas and an absence of outbreaks in ghana in spite of an abundance of a. aegypti mosquitoes, we established a continuous cell line from embryonic cells of a. aegypti collected in ghana and assessed its ... | 2020 | 33000384 |
| [extension of torch spectrum in ophthalmology: congenital zika, case report]. | zika is a flavivirus that can be transmitted transplacentally. eye abnormalities have been reported in 70% of zika cases, and 41.7% of them can occur in the absence of microcephaly. the most common ocular abnormalities are macular atrophy, optic atrophy and chorioretinal coloboma. the objective was to report the case of eye disorders associated with zika, acquired transplacentally, despite negative results for torch, and serology and pcr analyses for zika. | 2019 | 33001616 |
| zika virus-based immunotherapy enhances long-term survival of rodents with brain tumors through upregulation of memory t-cells. | zika virus (zikv) exhibits a tropism for brain tumor cells and has been used as an oncolytic virus to target brain tumors in mice with modest effects on extending median survival. recent studies have highlighted the potential for combining virotherapy and immunotherapy to target cancer. we postulated that zikv could be used as an adjuvant to enhance the long-term survival of mice with malignant glioblastoma and generate memory t-cells capable of providing long-term immunity against cancer remiss ... | 2020 | 33002018 |
| evs containing host restriction factor ifitm3 inhibited zikv infection of fetuses in pregnant mice through trans-placenta delivery. | zika virus (zikv) infection can lead to neurological complications and fetal defects, and it has attracted global public health concerns. effective treatment for zikv infection remains elusive, and a preventative vaccine is not yet available. therapeutics for fetuses need to overcome placenta barriers to reach the fetuses and require higher safety standards. in the present study, we engineered mammalian extracellular vesicles (evs) to deliver a host restriction factor, interferon-induced transme ... | 2020 | 33002418 |
| serological cross-reactivity using a sars-cov-2 elisa test in acute zika virus infection, colombia. | we investigated seroreactivity by using a commercial sars-cov-2 elisa test in samples collected from different groups of individuals, including patients diagnosed to have dengue, zika, and chikungunya infection between 2015 and 2019, from an endemic area in the caribbean colombian region. | 2020 | 33002616 |
| postnatal symptomatic zika virus infections in children and adolescents: a systematic review. | recent zika virus (zikv) outbreaks in the pacific and the americas have highlighted clinically significant congenital neurological abnormalities resulting from zikv infection in pregnancy. however, little is known about zikv infections in children and adolescents, a group that is potentially vulnerable to zikv neurovirulence. | 2020 | 33006989 |
| understanding the magnitude of risk associated with zika virus infection in pregnancy. | 2020 | 33007152 | |
| zika says no dice to dicer. | among the common flaviviruses that infect humans, zika virus, and the contemporary asian strain in particular, is distinctively associated with microcephaly. zeng et al. (2020) report in this issue of cell stem cell that strong binding and inhibition of human dicer enzyme by the capsid protein is a potential mechanism for this unique pathogenic potential. | 2020 | 33007231 |
| impact of intensive physiotherapy training for children with congenital zika syndrome: a retrospective cohort study. | to investigate the impact of intensive physiotherapy training on the motor function of children with congenital zika syndrome (czs). | 2020 | 33007306 |
| the zika virus individual participant data consortium: a global initiative to estimate the effects of exposure to zika virus during pregnancy on adverse fetal, infant, and child health outcomes. | this commentary describes the creation of the zika virus individual participant data consortium, a global collaboration to address outstanding questions in zika virus (zikv) epidemiology through conducting an individual participant data meta-analysis (ipd-ma). the aims of the ipd-ma are to (1) estimate the absolute and relative risks of miscarriage, fetal loss, and short- and long-term sequelae of fetal exposure; (2) identify and quantify the relative importance of different sources of heterogen ... | 2020 | 33007828 |
| evidence of zika virus horizontal and vertical transmission in aedes albopictus from spain but not infectious virus in saliva of the progeny. | aedes albopictus mosquitoes have been experimentally demonstrated to be a competent vector for zika virus (zikv) in different countries, but there are still some gaps related to the importance of ae. albopictus in zikv transmission. recent studies on spanish ae. albopictus populations showed controversial results for zikv transmission and no studies have been performed yet to detect infectious zikv in saliva of progeny of infected female mosquitoes. herein, the horizontal transmission (ht) and v ... | 2020 | 33008282 |
| identification of a pocket factor that is critical to zika virus assembly. | zika virus (zikv) is an emerging mosquito borne flavivirus and a major public health concern causing severe disease. due to the presence of a lipid membrane and structural heterogeneity, attaining an atomic resolution structure is challenging, but important to understand virus assembly and life cycle mechanisms that offer distinct targets for therapeutic intervention. we here use subvolume refinement to achieve a 3.4 å resolution structure and identify two distinct lipid moieties. the first aris ... | 2020 | 33009400 |
| sars-cov2-mediated suppression of nrf2-signaling reveals potent antiviral and anti-inflammatory activity of 4-octyl-itaconate and dimethyl fumarate. | antiviral strategies to inhibit severe acute respiratory syndrome coronavirus 2 (sars-cov2) and the pathogenic consequences of covid-19 are urgently required. here, we demonstrate that the nrf2 antioxidant gene expression pathway is suppressed in biopsies obtained from covid-19 patients. further, we uncover that nrf2 agonists 4-octyl-itaconate (4-oi) and the clinically approved dimethyl fumarate (dmf) induce a cellular antiviral program that potently inhibits replication of sars-cov2 across cell ... | 2020 | 33009401 |
| identification of the phytobioactive polygonum cuspidatum as an antiviral source for restricting dengue virus entry. | dengue virus (denv) is a mosquito-borne pathogen that is becoming a serious global threat, owing to its rising incidence in inter-tropical regions that yield over 50 million annual infections. there are currently no approved antiviral agents for the management of dengue, and recent shortcomings in its immunization called for immediate action to develop effective drugs with prophylactic ability to better manage its infection. in an attempt to discover novel antiviral sources, we identified the me ... | 2020 | 33009425 |
| zika virus-induced brain malformations in chicken embryos. | zika virus (zikv) was confirmed to be related to microcephaly in 2016. however, there is still a need for understanding the embryonic morphological changes induced by zikv and when they occur. here, chicken embryos were chosen as experimental model of zikv to evaluate virus-associated morphological alterations that might take place during embryonic development. | 2020 | 33009728 |
| inhibition of brazilian zikv strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide. | zika virus (zikv) infection during pregnancy is associated with a congenital syndrome. although the virus can be detected in human placental tissue and sexual transmission has been verified, it is not clear how the virus reaches the fetus. despite the emerging severity caused by zikv infection, no specific prophylactic and/or therapeutic treatment is available. the aim of the present study was to evaluate the effectiveness antiviral of nitazoxanide (ntz) in two important congenital transmission ... | 2020 | 33010209 |
| affinity-restricted memory b cells dominate recall responses to heterologous flaviviruses. | memory b cells (mbcs) can respond to heterologous antigens either by molding new specificities through secondary germinal centers (gcs) or by selecting preexisting clones without further affinity maturation. to distinguish these mechanisms in flavivirus infections and immunizations, we studied recall responses to envelope protein domain iii (diii). conditional deletion of activation-induced cytidine deaminase (aid) between heterologous challenges of west nile, japanese encephalitis, zika, and de ... | 2020 | 33010224 |
| classification of the interictal state with hypsarrhythmia from zika virus congenital syndrome and of the ictal state from epilepsy in childhood without hypsarrhythmia in eegs using entropy measures. | this paper intends to classify the interictal state with hypsarrhythmia in patients with zika virus congenital syndrome (zvcs) and of the ictal state in patients with epilepsy in childhood without the presence of hypsarrhythmia. hypsarrhythmia is a specific interictal chaotic morphology, and the correct distinction between these two eeg states is crucial to improving the cognitive development of these epileptic patients. the proposed approach was assessed using the proprietary database of casa n ... | 2020 | 33010735 |
| tam and tim receptors mrna expression in zika virus infected placentas. | to investigate the role of tyro3, axl and tim1 receptors in the zika virus (zikv) cycle, we determined their mrna expression in different placental sites of zikv infected tissue during pregnancy. unexpectedly, the zikv infection was not related with mrna upregulation of these receptors or changes in expression of type i and iii interferons in different placental sites. instead, a decrease of tyro3 mrna expression was observed in positive sites of zikv positive placentas in comparison to negative ... | 2020 | 33011564 |
| accurate detection of zika virus igg using a novel immune complex binding elisa. | accurate serological assays are urgently needed to support public health responses to zika virus (zikv) infection with its potential to cause foetal damage during pregnancy. current flavivirus serology for zikv infections lacks specificity due to cross-reacting antibodies from closely related other flaviviruses. in this study, we evaluated novel serological tests for accurate zikv igg detection. | 2020 | 33012038 |
| passive immunisation of convalescent human anti-zika plasma protects against challenge with new world zika virus in cynomolgus macaques. | zika virus (zikv) causes neurological complications in susceptible individuals, highlighted in the recent south american epidemic. natural zikv infection elicits host responses capable of preventing subsequent re-infection, raising expectations for effective vaccination. defining protective immune correlates will inform viral intervention strategies, particularly vaccine development. non-human primate (nhp) species are susceptible to zikv and represent models for vaccine development. the protect ... | 2020 | 33014434 |
| ante la duda, pregunta: a social marketing campaign to improve contraceptive access during a public health emergency. | during the 2016-2017 zika virus outbreak, preventing unintended pregnancy was recognized as a primary strategy to reduce adverse zika-related pregnancy and birth outcomes. to increase awareness and uptake of contraceptive services provided through the zika contraception access network (z-can) in puerto rico, a multi-strategy campaign called ante la duda, pregunta (aldp) was developed. the principal aim was to increase awareness of z-can services, which included same-day access to the full range ... | 2020 | 33016136 |
| early growth parameters as predictors of developmental delay among children conceived during the 2015-2016 zika virus outbreak in northeastern brazil. | identifying infants with congenital infection for early intervention will likely be challenging in future zika virus outbreaks. we investigated indicators of risk for developmental delay among children born with and without obvious manifestations of congenital zika virus infection. | 2020 | 33019699 |
| first confirmed occurrence of the yellow fever virus and dengue virus vector aedes (stegomyia) luteocephalus (newstead, 1907) in mozambique. | mozambique, same as many other tropical countries, is at high risk of arthropod-borne virus (arbovirus) diseases and recently two dengue virus (denv) outbreaks occurred in the northern part of the country. the occurrence of some important vector species, such as aedes (stegomyia) aegypti (linnaeus) and ae. (stg.) albopictus (skuse), besides several other sylvatic vectors, have been reported in the country, which may indicate that the transmission of some arboviruses of public health importance m ... | 2020 | 33019944 |
| acceptability of a hypothetical zika vaccine among women from colombia and spain exposed to zikv: a qualitative study. | zika virus (zikv) can cause pregnancy loss and congenital zika syndrome, among other poor health outcomes. the zikv epidemic in 2015-2017 disproportionately affected pregnant women in poor-resource settings. we aimed to understand perceptions and attitudes towards a hypothetical zikv vaccine, women's willingness to be vaccinated, and potential barriers and facilitators for vaccine acceptance in 1) migrant women living in spain who travelled to their countries of origin and were diagnosed with zi ... | 2020 | 33022907 |
| unidentified dengue serotypes in denv positive samples and detection of other pathogens responsible for an acute febrile illness outbreak 2016 in cajamarca, peru. | to describe the prevalence of dengue virus serotypes, as well as other viral and bacterial pathogens that cause acute febrile illness during an outbreak in cajamarca in 2016. | 2020 | 33023645 |
| gold nanoparticle/dna-based nanobioconjugate for electrochemical detection of zika virus. | the development of a stable nanobioconjugate based on gold nanoparticles (aunps) linked to single-strand dna (ssdna) is reported for amplification of the electrochemical signal of a zika virus (zikv) genetic material-based bioassay, with high sensitivity. the genosensor was assembled either at a screen-printed gold electrode (spaue) or a screen-printed carbon electrode decorated with hierarchical gold nanostructures (spce/au), with ru3+ as an electrochemical reporter. the genosensor response, in ... | 2020 | 33026568 |
| anti-zika virus and anti-usutu virus activity of human milk and its components. | the benefits of human milk are mediated by multiple nutritional, trophic, and immunological components, able to promote infant's growth, maturation of its immature gut, and to confer protection against infections. despite these widely recognized properties, breast-feeding represents an important mother-to-child transmission route of some viral infections. different studies show that some flaviviruses can occasionally be detected in breast milk, but their transmission to the newborn is still cont ... | 2020 | 33027261 |
| survival analysis in non-congenital neurological disorders related to dengue, chikungunya and zika virus infections in northeast brazil. | the northeast of brazil has experienced a triple epidemic, with the simultaneous circulation of dengue virus (denv), chikungunya virus (chikv) and zika virus (zikv), which may have contributed to the observed increase across this region of atypical forms of disease and deaths. in view of this fact, non-congenital neurological disorders related to arboviruses were compared with other etiologies, mortality and survival rates of patients admitted to referral neurology hospitals in pernambuco state, ... | 2020 | 33027393 |
| amyloid precursor protein is a restriction factor that protects against zika virus infection in mammalian brains. | zika virus (zikv) is a neurotropic flavivirus that causes several diseases including birth defects such as microcephaly microcephaly. intrinsic immunity is known to be a frontline defense against viruses through host anti-viral restriction factors. limited knowledge is available on intrinsic immunity against zikv in brains. amyloid precursor protein (app) is predominantly expressed in brains and implicated in the pathogenesis of alzheimer's diseases. we have found that zikv interacts with app, a ... | 2020 | 33028637 |
| zika virus-like particles bearing covalent dimer of envelope protein protect mice from lethal challenge. | zika virus (zikv) envelope (e) protein is the major target of neutralizing antibodies in infected host, and thus represents a candidate of interest for vaccine design. however, a major concern in the development of vaccines against zikv and the related dengue virus is the induction of cross-reactive poorly neutralizing antibodies that can cause antibody-dependent enhancement (ade) of infection. this risk necessitates particular care in vaccine design. specifically, the engineered immunogens shou ... | 2020 | 33028720 |
| health-related quality of life in neurological disorders most commonly associated with zika-virus infection: a systematic review. | 2020 | 33032786 | |
| clinical features and laboratory diagnosis of emerging arthropod-transmitted viruses: a report from the pan american society for clinical virology clinical practice committee. | arthropod-borne viruses (arboviruses) are an increasing global threat due to their ability to cause human disease and their expanding geographical distribution. they circulate in nature between arthropod vectors and vertebrate hosts. infection of susceptible human hosts leads to harmful developmental and neurological manifestations. arboviruses have caused recent outbreaks with significant public health implications, such as the zika virus outbreak in the western hemisphere which caused fetal ab ... | 2020 | 33035733 |
| zika virus and arthritis/arthralgia: a systematic review and meta-analysis. | dengue, chikungunya and zika viruses share similar disease features, rendering them difficult to distinguish clinically. incapacitating arthralgia/arthritis is a specific manifestation associated with chikungunya virus infection. however, the profile of arthralgia/arthritis in zika virus (zikv) cases has not been well characterized. articles were extracted from pubmed and scopus databases reporting original data from patients with arthralgia/arthritis, according to the cochrane collaboration. fo ... | 2020 | 33036370 |
| warming temperatures could expose more than 1.3 billion new people to zika virus risk by 2050. | in the aftermath of the 2015 pandemic of zika virus (zikv), concerns over links between climate change and emerging arboviruses have become more pressing. given the potential that much of the world might remain at risk from the virus, we used a previously established temperature-dependent transmission model for zikv to project climate change impacts on transmission suitability risk by mid-century (a generation into the future). based on these model predictions, in the worst-case scenario, over 1 ... | 2020 | 33037740 |
| pediatric neurodevelopment by prenatal zika virus exposure: a cross-sectional study of the microcephaly epidemic research group cohort. | the implications of congenital zika virus (zikv) infections for pediatric neurodevelopment and behavior remain inadequately studied. the aim of this study is to investigate patterns of neurodevelopment and behavior in groups of children with differening severities of zikv-related microcephaly and children with prenatal zikv exposure in the absence of microcephaly. | 2020 | 33038931 |
| guillain-barré syndrome in a patient previously diagnosed with covid-19. | as the covid-19 pandemic continues to progress, the medical community is rapidly trying to identify complications and patterns of disease to improve patient outcomes. in a recent systematic review, it has been reported that isolated cases of guillain-barre syndrome (gbs) have occurred secondary to covid-19 infection. gbs is defined as a rare, but potentially fatal, immune mediated disease of peripheral nerves and nerve roots that is usually triggered by infections. the incidence of gbs can there ... | 2020 | 33041119 |
| itaconic acid hybrids as potential anticancer agents. | in this paper, we report the synthesis of novel hybrids 2-14 based on itaconic acid and fluoroaniline, pyridine, indole and quinoline scaffolds. itaconic acid is a naturally occurring compound with a michael acceptor moiety, a key structural feature in several anticancer and antiviral drugs, responsible for the covalent binding of a drug to the cysteine residue of a specific protein. aromatic parts of the hybrids also come from the substances reported as anticancer or antiviral agents. the synth ... | 2020 | 33043404 |
| molecular docking and antiviral activities of plant derived compounds against zika virus. | zika virus (zikv), a recently emerged pathogen of the genus flavivirus causes guillain-barré syndrome and microcephaly in fetus and newborns . until date, there are no licensed vaccine or approved drug to treat zikv infection. thus, in this study, 5550 phytochemicals retrieved from various databases were subjected for molecular docking in discovery studio v.4.0 against the zikv helicase protein and envelope protein domain iii. in addition, in silico admet and density function theory studies were ... | 2020 | 33045342 |
| live vaccine infection burden elicits adaptive humoral and cellular immunity required to prevent zika virus infection. | the emergence of zika virus (zikv) as an important cause of congenital and childhood developmental disorders presents another challenge to global health. efforts to develop a zika vaccine have begun although vaccine development against flaviviruses, of which zikv belongs to, has proven to be time-consuming and challenging. defining the vaccine attributes that elicit adaptive immune response necessary for preventing zikv infection could provide an evidence-based guide to zika vaccine development. | 2020 | 33045466 |
| a dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in china. | mosquito-borne viruses such as zika virus, japanese encephalitis virus and dengue virus present an increasing global health concern. however, in-depth knowledge of the distribution and diversity of mosquito-associated viruses and their related vectors remains limited, especially for china. to promote their understanding, we present the first comprehensive dataset of the distribution and diversity of these viruses and their related vectors in china (including taiwan, hong kong and macau). data wa ... | 2020 | 33051449 |
| systematic discovery and functional interrogation of sars-cov-2 viral rna-host protein interactions during infection. | severe acute respiratory syndrome coronavirus 2 (sars-cov-2) is the cause of a pandemic with growing global mortality. there is an urgent need to understand the molecular pathways required for host infection and anti-viral immunity. using comprehensive identification of rna-binding proteins by mass spectrometry (chirp-ms), we identified 309 host proteins that bind the sars-cov-2 rna during active infection. integration of this data with viral chirp-ms data from three other positive-sense rna vir ... | 2020 | 33052334 |
| controlling the development of the dengue vector aedes aegypti using hr3 rnai transgenic chlamydomonas. | the aedes aegypti mosquito plays an important role in the spread of diseases, including epidemic ones, such as dengue fever, zika virus disease, yellow fever, and chikungunya disease. to control the population of ae.aegypti, we transferred an hr3 rnai fragment into the microalgae chlamydomonas, which serves as food for ae.aegypti larvae. results showed that the hr3 rnai transgenic algal strains were lethal to ae.aegypti. the integumentary system of larvae fed with hr3 rnai transgenic algal strai ... | 2020 | 33052930 |
| reversion to ancestral zika virus ns1 residues increases competence of aedes albopictus. | both mosquito species-specific differences and virus strain -specific differences impact vector competence. previous results in our laboratory with individual populations of n. american mosquitoes support studies suggesting aedes aegypti are more competent than ae. albopictus for american zika virus (zikv) strains and demonstrate that u.s. ae. albopictus have higher competence for an ancestral asian zikv strain. a982v, an amino acid substitution in the ns1 gene acquired prior to the american out ... | 2020 | 33052957 |
| updating zika diagnostic methods: the point-of-care approach. | zika virus (zikv) has gained great importance worldwide since the past epidemic that occurred in 2015 in brazil. early identification of zikv is critical to minimize transmission and prevents potentially devastating consequences, including microcephaly in neonates of infected women, congenital blindness, or guillain-barré syndrome. however, this is not an easy task, considering that approximately 80% of zikv infection cases are asymptomatic or oligosymptomatic, there are diverse modes of transmi ... | 2020 | 33053568 |
| cross-sectional study of the anthropometric characteristics of children with congenital zika syndrome up to 12 months of life. | little is known about physical development of children with congenital zika syndrome (czs). this study aims to evaluate the anthropometric characteristics of children with czs up to 12 months. | 2020 | 33054749 |
| vertical transmission of zika virus in aedes albopictus. | zika virus (zikv) is an arthropod-borne flavivirus transmitted by aedes mosquitoes. aedes albopictus is an important vector of zikv worldwide. to date, most experiments have focused on the vertical transmission of zikv in ae. aegypti, while studies on ae. albopictus are very limited. to explore vertical transmission in ae. albopictus, a series of laboratory studies were carried out. | 2020 | 33057411 |
| host gene expression modulated by zika virus infection of human-293 cells. | the hek-293 cell line was created in 1977 by transformation of primary human embryonic kidney cells with sheared adenovirus type 5 dna. a previous study determined that the hek-293 cells have neuronal markers rather than kidney markers. in this study, we tested the hypothesis whether zika virus (zikv), a neurotropic virus, is able to infect and replicate in the hek-293 cells. we show that the hek-293 cells infected with zikv support viral replication as shown by indirect immunofluorescence (ifa) ... | 2020 | 33059318 |
| discovery of zika virus ns2b/ns3 inhibitors that prevent mice from life-threatening infection and brain damage. | zika virus (zikv) infection, which initially was endemic only in africa and asia, is rapidly spreading throughout europe, oceania, and the americas. although there have been enormous efforts, there is still no approved drug to treat zikv infection. herein, we report the synthesis and biological evaluation of agents with noncompetitive mechanism of the zikv ns2b/ns3 protease inhibition through the binding to an allosteric site. compounds 1 and 2 showed potent activity in both enzymatic and cellul ... | 2020 | 33062166 |
| emergence of zika virus: is morocco concerned? | 2016 | 33065892 | |
| emergence of zika virus: past detection of antibodies against zika virus does not mean active circulation of the virus. | 2016 | 33065893 | |
| a recombinant zika virus envelope protein with mutations in the conserved fusion loop leads to reduced antibody cross-reactivity upon vaccination. | zika virus (zikv) is a zoonotic, human pathogenic, and mosquito-borne flavivirus. its distribution is rapidly growing worldwide. several attempts to develop vaccines for zikv are currently ongoing. central to most vaccination approaches against flavivirus infections is the envelope (e) protein, which is the major target of neutralizing antibodies. insect-cell derived, recombinantly expressed variants of e from the flaviviruses west nile and dengue virus have entered clinical trials in humans. al ... | 2020 | 33066262 |
| zikv viral proteins and their roles in virus-host interactions. | the re-emergence of zika virus (zikv) and its associated neonatal microcephaly and guillain-barré syndrome have led the world health organization to declare a global health emergency. until today, many related studies have successively reported the role of various viral proteins of zikv in the process of zikv infection and pathogenicity. these studies have provided significant insights for the treatment and prevention of zikv infection. here we review the current research advances in the functio ... | 2020 | 33068285 |