Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| dientamoeba fragilis in gaza strip: a neglected protozoan parasite. | the aim of this study was to detect dientamoeba fragilis by iron haematoxylin stain, as well as its prevalence, and association between d. fragilis infection and diarrhoea among patients attending al-nuseirate refugee camp clinic, gaza strip. | 2013 | 23914238 |
| sexually transmitted infections manifesting as proctitis. | there is a rising incidence of several sexually transmitted infections (stis), many of which can present with proctitis. causative organisms include neisseria gonorrhoeae, chlamydia trachomatis, herpes simplex virus, treponema pallidum (syphilis), giardia lamblia (giardiasis) and entamoeba histolytica (amoebiasis). this paper outlines important clinical discriminators and key investigations to distinguish these organisms from non-infective pathology that include inflammatory bowel disease, solit ... | 2013 | 23914292 |
| [water pollution and health risks at yaoundé, cameroon]. | the aim of this article consists of developing an ecosystemic approach to collect information about groundwater pollution in order to determine its origin and infer the health risks in mingoa's watershed through the study of 12 spontaneous settlements. from an environmental point of view, family interviews and direct observations allowed us to inventory springs, wells and latrines, and to collect information. so, we have selected, on the basis of predefined criteria, 21 wells and springs for phy ... | 2013 | 23916209 |
| [drug therapy of infectious diarrhea. part 2: chronic diarrhea]. | diarrheal diseases are among the most common diseases worldwide. in this review the current treatment recommendations for acute (part 1) and chronic (part 2) infectious diarrhea are summarized and typical enteropathogens are discussed. the second part of the article describes chronic diarrhea, its related pathogens and treatment. in contrast to acute diarrhea which is mainly caused by viral and typical bacterial pathogens, chronic diarrhea has mainly non-infectious origins. protozoal pathogens, ... | 2013 | 23917963 |
| enteric pathogens in hiv infected and hiv uninfected individuals with diarrhea in pune. | diarrhea in hiv infected individuals is a common complication seen in about 90% of patients in developing countries. the objective of this study was to identify enteric pathogens in hiv infected and hiv uninfected individuals in pune. | 2013 | 23918286 |
| microbiological and parasitological investigation among food handlers in hotels in the dead sea area, jordan. | intestinal parasitic and bacterial infections constitute a major health issue in developing countries. the present study investigates and assesses infection rates among food handlers with intestinal parasites and microbial agents in luxurious hotels in the dead sea area of jordan. | 2014 | 23933293 |
| robust gene silencing mediated by antisense small rnas in the pathogenic protist entamoeba histolytica. | rna interference uses small rnas (srna), which target genes for sequence-specific silencing. the parasite entamoeba histolytica contains an abundant repertoire of 27 nt antisense (as) srna with 5'-polyphosphate termini, but their roles in regulating gene expression have not been well established. we demonstrate that a gene-coding region to which large numbers of as srnas map can serve as a 'trigger' and silence the gene fused to it. silencing is mediated by generation of as srnas with 5'-polypho ... | 2013 | 23935116 |
| mini-flotac, an innovative direct diagnostic technique for intestinal parasitic infections: experience from the field. | soil-transmitted helminths and intestinal protozoa infection are widespread in developing countries, yet an accurate diagnosis is rarely performed. the aim of this study was to evaluate the recently developed mini-flotac method and to compare with currently more widely used techniques for the diagnosis of intestinal parasitic infections in different settings. | 2013 | 23936577 |
| imported amoebic liver abscess in france. | worldwide, amoebic liver abscess (ala) can be found in individuals in non-endemic areas, especially in foreign-born travelers. | 2013 | 23951372 |
| efficacies of two in-house indirect elisas for diagnosis of amoebic liver abscess. | entamoeba histolytica causes amoebic diarrhoea, colitis and liver abscess (ala). diagnosis of ala is difficult, as most patients do not have simultaneous intestinal amoebic infection. at hospital universiti sains malaysia (husm), diagnosis of ala relies on a combination of clinical findings, ultrasound examination of the liver and serodiagnosis using a commercial kit. in this study, two in-house indirect elisas were developed and evaluated. one of the in-house assays utilises e. histolytica crud ... | 2013 | 23959490 |
| current laboratory diagnosis of opportunistic enteric parasites in human immunodeficiency virus-infected patients. | diarrhea is a major cause of morbidity and mortality in human immunodeficiency virus (hiv)-infected individuals. opportunistic enteric parasitic infections are encountered in 30-60% of hiv seropositive patients in developed countries and in 90% of patients in developing countries. once the cd4(+) cell count drops below 200 cells/μl, patients are considered to have developed acquired immunodeficiency syndrome (aids), with the risk of an aids-defining illness or opportunistic infection significant ... | 2013 | 23961436 |
| evidence for a bacterial lipopolysaccharide-recognizing g-protein-coupled receptor in the bacterial engulfment by entamoeba histolytica. | entamoeba histolytica is the causative agent of amoebic dysentery, a worldwide protozoal disease that results in approximately 100,000 deaths annually. the virulence of e. histolytica may be due to interactions with the host bacterial flora, whereby trophozoites engulf colonic bacteria as a nutrient source. the engulfment process depends on trophozoite recognition of bacterial epitopes that activate phagocytosis pathways. e. histolytica gpcr-1 (ehgpcr-1) was previously recognized as a putative g ... | 2013 | 23975887 |
| effect of the silencing of the ehcp112 gene on the in vitro virulence of entamoeba histolytica. | entamoeba histolytica is an intestinal protozoan parasite that causes amoebiasis in humans, affecting up to 50 million people worldwide each year and causing 40,000 to 100,000 deaths annually. ehcp112 is a cysteine proteinase of e. histolytica able to disrupt cell monolayers and digest extracellular matrix proteins, it is secreted by trophozoites and it can be active in a wide range of temperature and ph. these characteristics have encouraged the use of ehcp112 in the design and production of po ... | 2013 | 23981435 |
| real-time pcr assay in differentiating entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii infections in orang asli settlements in malaysia. | amebiasis caused by entamoeba histolytica is the third leading cause of death worldwide. this pathogenic amoeba is morphologically indistinguishable from e. dispar and e. moshkovskii, the non-pathogenic species. polymerase chain reaction is the current method of choice approved by world health organization. real-time pcr is another attractive molecular method for diagnosis of infectious diseases as post-pcr analyses are eliminated and turnaround times are shorter. the present work aimed to compa ... | 2013 | 23985047 |
| high prevalence of diarrhoegenic intestinal parasite infections among non-art hiv patients in fitche hospital, ethiopia. | hiv infection has been modifying both the epidemiology and outcome of parasite infections. hence, this study was undertaken to determine the prevalence of cryptosporidium and other intestinal parasite infections among hiv positives with and without antiretroviral treatment(art) and its association with cd4+ t-cell count. | 2013 | 23991132 |
| amoebic dysentery. | amoebic dysentery is caused by the protozoan parasite entamoeba histolytica. it is transmitted in areas where poor sanitation allows contamination of drinking water and food with faeces. in these areas, up to 40% of people with diarrhoea may have amoebic dysentery. | 2013 | 23991750 |
| effect of the sesquiterpene lactone incomptine a in the energy metabolism of entamoeba histolytica. | entamoeba histolytica is the causative agent of human amoebiasis, which mainly affects developing countries. although several drugs are effective against e. histolytica trophozoites, the control of amoebiasis requires the development of new and better alternative therapies. medicinal plants have been the source of new molecules with remarkable antiprotozoal activity. incomptine a isolated from decachaeta incompta leaves, is a sesquiterpene lactone of the heliangolide type which has the major in ... | 2013 | 23994114 |
| molecular basis for benzimidazole resistance from a novel β-tubulin binding site model. | benzimidazole-2-carbamate derivatives (bzcs) are the most commonly used antiparasitic drugs for the treatment of protozoan and helminthic infections. bzcs inhibit the microtubule polymerization mechanism through binding selectively to the β-tubulin subunit in which mutations have been identified that lead to drug resistance. currently, the lack of crystallographic structures of β-tubulin in parasites has limited the study of the binding site and the analysis of the resistance to bzcs. recently, ... | 2013 | 23995453 |
| immunogenicity and protective efficacy of heparan sulphate binding proteins of entamoeba histolytica in a guinea pig model of intestinal amoebiasis. | entamoeba histolytica infection is associated with considerable morbidity and mortality in the form of intestinal and extraintestinal amoebiasis. no vaccine is yet available for amoebiasis. heparan sulphate binding proteins (hsbps) from e. histolytica were evaluated for immunogenicity and protective efficacy in a guinea pig model. animals were immunized subcutaneously with 30μg of hsbp by three weekly inoculations. the immunogenicity of hsbp was determined by antibody response (igg, igm and iga) ... | 2013 | 24007700 |
| entamoeba histolytica p-glycoprotein (ehpgp) inhibition, induce trophozoite acidification and enhance programmed cell death. | programmed cell death (pcd) is induced in entamoeba histolytica by a variety of stimuli in vitro and in vivo. in mammals, intracellular acidification serves as a global switch for inactivating cellular processes and initiates molecular mechanisms implicated in the destruction of the genome. in contrast, intracellular alkalinization produced by p-glycoprotein overexpression in multidrug-resistant cells has been related to apoptosis resistance. our previous studies showed that overexpression of e. ... | 2013 | 24012862 |
| infection-induced anaemia: a cross-sectional study of 14,636 german travellers aged 20-49 years. | anaemia is a frequently diagnosed condition which can develop as a consequence of numerous factors, including infectious diseases (ids). travelling, especially in sub-/tropical regions, leads to an elevated risk of contracting ids. the aim of our study was to assess the epidemiological significance of ids in inducing anaemia among a large cohort of returned travellers. | 2013 | 24014235 |
| [amebic liver abscess and late recurrence with no travel in an endemic area]. | amebic liver abscess is the main complication of amebic dysentery. recurrences after treatment and apparent healing are very uncommon. the purpose of this report is to describe the case of a patient with a very late relapse of an amebic liver abscess, 10 years after the first episode. this recurrence seems due to an incomplete initial treatment. this case illustrates the reason for and importance of complying with the current therapeutic strategy: nitroimidazole followed by a luminal agent to er ... | 2016 | 24026002 |
| transcriptome analysis of encystation in entamoeba invadens. | encystation is an essential differentiation process for the completion of the life cycle of a group of intestinal protozoa including entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis. however, regulation of gene expression during encystation is poorly understood. to comprehensively understand the process at the molecular level, the transcriptomic profiles of e. invadens, which is a related reptilian species that causes an invasive disease similar to that of e ... | 2013 | 24040350 |
| etiology of diarrhea in bangladeshi infants in the first year of life analyzed using molecular methods. | diarrhea causes enormous morbidity and mortality in developing countries, yet the relative importance of multiple potential enteropathogens has been difficult to ascertain. | 2013 | 24041797 |
| prevalence of intestinal parasitic infection among hiv positive persons who are naive and on antiretroviral treatment in hiwot fana specialized university hospital, eastern ethiopia. | background. intestinal parasitic infection affects the health and quality of life of people living with hiv. this study was aimed to determine the prevalence of intestinal parasites among hiv positive individuals who are naive and who are on antiretroviral treatment (art) in hiwot fana specialized university hospital, eastern ethiopia. methods. a comparative cross-sectional study was conducted on 371 (112 art-naive group and 259 on art) hiv positive individuals. stool specimens were collected an ... | 2013 | 24052888 |
| [clinical case of the month. amoebic liver abscess contracted in india with diagnosis confirmed by pcr]. | amoebiasis is a disease of parasitic origin responsible for dysentery and extra-intestinal complications. the infection is by entamoeba histolytica, an amoeba whose geographical distribution is cosmopolitan, but that is more prevalent in tropical areas. only a few infections are symptomatic and some of them may cause extra-intestinal complications. hepatic amoebiasis is the most frequently observed. we report the case of a belgian woman who developed an amoebic liver abscess after returning from ... | 2015 | 24053104 |
| entamoeba histolytica contains an occludin-like protein that can alter colonic epithelial barrier function. | the exact mechanism by which entamoeba histolytica disrupts the human colonic epithelium and invades the mucosa has yet to be clearly elucidated. e. histolytica produces a diverse array of putative virulent factors such as glycosidase, cysteine proteinases and amebapore that can modulate and/or disrupt epithelial barrier functions. however, it is currently thought that e. histolytica produces numerous other molecules and strategies to disrupt colonic mucosal defenses. in this study, we document ... | 2013 | 24058468 |
| a test of agent-based models as a tool for predicting patterns of pathogen transmission in complex landscapes. | landscape complexity can mitigate or facilitate host dispersal, influencing patterns of pathogen transmission. spatial transmission of pathogens through landscapes, therefore, presents an important but not fully elucidated aspect of transmission dynamics. using an agent-based model (link) that incorporates gis data, we examined the effects of landscape information on the spatial patterns of host movement and pathogen transmission in a system of long-tailed macaques and their gut parasites. we fi ... | 2013 | 24063811 |
| exploring the possible role of lysine acetylation on entamoeba histolytica virulence: a focus on the dynamics of the actin cytoskeleton. | cytoskeleton remodeling can be regulated, among other mechanisms, by lysine acetylation. the role of acetylation on cytoskeletal and other proteins of entamoeba histolytica has been poorly studied. dynamic rearrangements of the actin cytoskeleton are crucial for amebic motility and capping formation, processes that may be effective means of evading the host immune response. here we report the possible effect of acetylation on the actin cytoskeleton dynamics and in vivo virulence of e. histolytic ... | 2013 | 24078923 |
| intestinal parasitic infection in bhil tribe of rajasthan, india. | a total of 224 bhil tribal individuals (115 males and 109 females) of different age groups inhabiting tribal rural areas of udaipur district of rajasthan, india were investigated for the prevalence of intestinal parasitic (protozoan and helminths) infections. fresh stool samples of these tribal subjects were examined microscopically by direct wet smear with saline and 1 % lugol's iodine and formaline ether concentration. of these 116 (51.78 %) were found to be infected with diverse species of in ... | 2012 | 24082517 |
| [an update on amebiasis]. | the description of entamoeba dispar, and the recovery of entamoeba moshkovskii from humans had a major impact in the epidemiology and clinical management of amebiasis. infections range from asymptomatic colonization to hemorrhagic colitis and extra-intestinal diseases. only a minority of amebiasis patients progress to the development of disease. recent studies suggest that susceptibility to infection, and its outcome is influenced by the host, parasite genotype, and environment. the identificati ... | 2013 | 24089276 |
| strategies to discover the structural components of cyst and oocyst walls. | cysts of giardia lamblia and entamoeba histolytica and oocysts of toxoplasma gondii and cryptosporidium parvum are the infectious and sometimes diagnostic forms of these parasites. to discover the structural components of cyst and oocyst walls, we have developed strategies based upon a few simple assumptions. briefly, the most abundant wall proteins are identified by monoclonal antibodies or mass spectrometry. structural components include a sugar polysaccharide (chitin for entamoeba, β-1,3-link ... | 2013 | 24096907 |
| homologous recombination occurs in entamoeba and is enhanced during growth stress and stage conversion. | homologous recombination (hr) has not been demonstrated in the parasitic protists entamoeba histolytica or entamoeba invadens, as no convenient method is available to measure it. however, hr must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from trophozoite to cyst. here we show the up regulation of mitotic and meiotic hr genes in entamoeba during serum starvation, and encystation. to directly demonstrate hr we use a simple pcr-based method ... | 2013 | 24098652 |
| historical perspective on developmental concepts and terminology. | in their ontogeny and phylogeny all living beings are historical entities. the revolution in biology of the 18th and 19th centuries that did away with the scala naturae according to which we humans, the acme of creation, "made a little lower than the angels," also led to the gradual realization that a humble one-celled protist ("protoctist"), such as entamoeba histolytica of ill repute [margulis and chapman, ] has the same 4-billion-year phylogeny as that of homo sapiens, vivid testimony to comm ... | 2013 | 24123982 |
| effectiveness of latex agglutination slide test in the diagnosis of imported invasive amoebiasis in the emergency department. | we compared a latex agglutination test (lat) with enzyme-linked immunosorbent assay and indirect hemagglutination assay in the diagnosis of invasive amoebiasis. a retrospective biological records review has included 639 patients for whom these three serological tests were performed. the sensitivity of the lat was 97.8% and the specificity was 97%. | 2013 | 24125923 |
| added value of multiplex luminex gastrointestinal pathogen panel (xtag® gpp) testing in the diagnosis of infectious gastroenteritis. | the luminex gastrointestinal pathogen panel (xtag(®) gpp) detects in one assay the most common gastroenteritis-causing pathogens and toxins, namely adenovirus 40/41, norovirus genogroup (ng) i/ii, rotavirus a, clostridium difficile toxin a/b, campylobacter sp., escherichia coli o157, enterotoxigenic e. coli heat-labile enterotoxin/heat-stable enterotoxin, salmonella sp., shiga-toxin producing e. coli, shiga-like toxin (stx)1/2, shigella sp., vibrio cholerae, yersinia enterocolitica, cryptosporid ... | 2014 | 24131399 |
| the cell surface proteome of entamoeba histolytica. | surface molecules are of major importance for host-parasite interactions. during entamoeba histolytica infections, these interactions are predicted to be of prime importance for tissue invasion, induction of colitis and liver abscess formation. to date, however, little is known about the molecules involved in these processes, with only about 20 proteins or protein families found exposed on the e. histolytica surface. we have therefore analyzed the complete surface proteome of e. histolytica. usi ... | 2014 | 24136294 |
| the expanding superfamily of gelsolin homology domain proteins. | the gelsolin homology (gh) domain has been found to date exclusively in actin-binding proteins. in humans, three copies of the domain are present in capg, five copies in supervillin, and six copies each in adseverin, gelsolin, flightless i and the villins: villin, advillin and villin-like protein. caenorhabditis elegans contains a four-gh-domain protein, gsnl-1. these architectures are predicted to have arisen from gene triplication followed by gene duplication to result in the six-domain protei ... | 2013 | 24155256 |
| detection of giardia lamblia, cryptosporidium spp. and entamoeba histolytica in clinical stool samples by using multiplex real-time pcr after automated dna isolation. | diagnosis of intestinal parasites in stool samples is generally still carried out by microscopy; however, this technique is known to suffer from a low sensitivity and is unable to discriminate between certain protozoa. in order to overcome these limitations, a real-time multiplex pcr was evaluated as an alternative approach for diagnosing giardia lamblia, cryptosporidium spp. and entamoeba histolytica in stool samples.therefore, a total of 631 faecal samples were analysed both by microscopy as w ... | 2016 | 24156218 |
| role of leptin-mediated colonic inflammation in defense against clostridium difficile colitis. | the role of leptin in the mucosal immune response to clostridium difficile colitis, a leading cause of nosocomial infection, was studied in humans and in a murine model. previously, a mutation in the receptor for leptin (lepr) was shown to be associated with susceptibility to infectious colitis and liver abscess due to entamoeba histolytica as well as to bacterial peritonitis. here we discovered that european americans homozygous for the same lepr q223r mutation (rs1137101), known to result in d ... | 2014 | 24166957 |
| synthesis and antiprotozoal activity of nitazoxanide-n-methylbenzimidazole hybrids. | a series of a novel hybrid compounds between nitazoxanide and n-methylbenzimidazole were synthesized starting from the corresponding n-methyl-2-nitroanilines. the new hybrid compounds (1-13) were evaluated in vitro against giardia intestinalis, entamoeba histolytica, trichomonas vaginalis. ntz, mtz and abz were used as drug standards. experimental evaluations revealed all of the new compounds (1-13) were active and showed strong activity against the three protozoa, particularly with e. histolyti ... | 2013 | 24183540 |
| correlation between genotypes of trna-linked short tandem repeats in entamoeba nuttalli isolates and the geographical distribution of host rhesus macaques. | several polymorphic markers, including serine-rich protein genes, have been used for the genotyping of isolates from the morphologically indistinguishable protozoan parasites entamoeba histolytica, entamoeba dispar, and entamoeba nuttalli. genotypes of trna-linked short tandem repeats (strs) are highly polymorphic, but the correlation with geographical distribution is unknown. we have recently isolated 15 e. nuttalli strains from wild rhesus macaques in four locations in kathmandu valley, nepal. ... | 2014 | 24185530 |
| differentiation of entamoeba histolytica/entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the sanliurfa province. | we aimed to diagnose amebiasis and also identify entamoeba histolytica (e. histolytica) and entamoeba dispar (e. dispar) in patients with gastrointestinal symptoms in an endemic region in turkey. | 2013 | 24192618 |
| influence of parasite density and sample storage time on the reliability of entamoeba histolytica-specific pcr from formalin-fixed and paraffin-embedded tissues. | we report on the reliability of polymerase chain reaction (pcr) for the detection of entamoeba histolytica from formalin-fixed, paraffin-embedded tissue in comparison with microscopy and have determined predictors that may influence pcr results. e. histolytica-specific and entamoeba dispar-specific real-time pcr and microscopy from adjacent histologic sections were performed using a collection of formalin-fixed, paraffin-embedded tissue specimens obtained from patients with invasive amebiasis. s ... | 2013 | 24193008 |
| detection of intestinal protozoa in the clinical laboratory. | despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. microscopy is, however, labor-intensive and requires a skilled technologist. new, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including giardia lamblia (syn. g. intestinalis/g. duodenalis) and cryptosporidium spp., using technologies that, if expanded, could effectively comple ... | 2014 | 24197877 |
| the ribosomal rna transcription unit of entamoeba invadens: accumulation of unprocessed pre-rrna and a long non coding rna during encystation. | the ribosomal rna genes in entamoeba spp. are located on extrachromosomal circular molecules. unlike model organisms where rrna transcription stops during growth stress, entamoeba histolytica continues transcription; but unprocessed pre-rrna accumulates during stress, along with a novel class of circular transcripts from the 5'-external transcribed spacer (ets). to determine the fate of rrna transcription during stage conversion between trophozoite to cyst we analyzed entamoeba invadens, a model ... | 2014 | 24200639 |
| intestinal parasitosis in school children of lalitpur district of nepal. | enteric parasites are the most common cause of parasitic diseases and cause significant morbidity and mortality, particularly in developing countries like nepal. the objective of this study was to estimate the prevalence and risk factors of intestinal parasitic infections among school going children of lalitpur district of nepal. | 2013 | 24207086 |
| prevalence of isosporiasis in relation to cd4 cell counts among hiv-infected patients with diarrhea in odisha, india. | to determine the prevalence of isospora belli and its correlation with cd4+ cell counts in hiv-positive patients with diarrhea in this region. | 2013 | 24223376 |
| analysis of the protein phosphotome of entamoeba histolytica reveals an intricate phosphorylation network. | phosphorylation is the most common mechanism for the propagation of intracellular signals. protein phosphatases and protein kinases play a dynamic antagonistic role in protein phosphorylation. protein phosphatases make up a significant fraction of eukaryotic proteome. in this article, we report the identification and analysis of protein phosphatases in the intracellular parasite entamoeba histolytica. based on an in silico analysis, we classified 250 non-redundant protein phosphatases in e. hist ... | 2013 | 24236039 |
| deciphering molecular mechanisms of mrna metabolism in the deep-branching eukaryote entamoeba histolytica. | although extraordinary rapid advance has been made in the knowledge of mechanisms regulating messenger rna (mrna) metabolism in mammals and yeast, little information is known in deep-branching eukaryotes. the complete genome sequence of entamoeba histolytica, the protozoan parasite responsible for human amoebiasis, provided a lot of information for the identification and comparison of regulatory sequences and proteins potentially involved in mrna synthesis, processing, and degradation. here, we ... | 2014 | 24249245 |
| gal-lectin-dependent contact activates the inflammasome by invasive entamoeba histolytica. | entamoeba histolytica (eh) is an extracellular protozoan parasite of the human colon, which occasionally breaches the intestinal barrier. eradicating ameba that invades is essential for host survival. a defining but uncharacterized feature of amebic invasion is direct contact between ameba and host cells. this event corresponds with a massive pro-inflammatory response. to date, pathogen recognition receptors (prrs) that are activated by contact with viable eh are unknown. here we show that the i ... | 2014 | 24253103 |
| prevalence and risk factors for intestinal parasites in food handlers, southern brazil. | in this study, the prevalence and risk factors for enteroparasites were determined in food handlers from maringá, paraná state, southern brazil. fecal and subungual materials of 150 street food vendors were analyzed by the methods of lutz, faust, and mello, respectively. a questionnaire on hygiene and sanitary conditions of the workplace and of domicile was applied. the prevalence of enteroparasites was 28%, and the protozoa infection was more expressive (21.3%) than by helminths (6.7%), but wit ... | 2014 | 24256564 |
| fascioliasis and intestinal parasitoses affecting schoolchildren in atlixco, puebla state, mexico: epidemiology and treatment with nitazoxanide. | the atlixco municipality, puebla state, at a mean altitude of 1840 m, was selected for a study of fasciola hepatica infection in schoolchildren in mexico. this area presents permanent water collections continuously receiving thaw water from popocatepetl volcano (5426 m altitude) through the community supply channels, conforming an epidemiological scenario similar to those known in hyperendemic areas of andean countries. | 2013 | 24278492 |
| monocyte locomotion inhibitory factor produced by e. histolytica improves motor recovery and develops neuroprotection after traumatic injury to the spinal cord. | monocyte locomotion inhibitory factor (mlif) is a pentapeptide produced by entamoeba histolytica that has a potent anti-inflammatory effect. either mlif or phosphate buffered saline (pbs) was administered directly onto the spinal cord (sc) immediately after injury. motor recovery was evaluated. we also analyzed neuroprotection by quantifying the number of surviving ventral horn motor neurons and the persistence of rubrospinal tract neurons. to evaluate the mechanism through which mlif improved t ... | 2013 | 24294606 |
| globalised world, globalised diseases: a case report on an amoebiasis-associated colon perforation. | in 2010 the world health organisation estimated the number of infections with entamoeba histolytica at about 50 million cases including 100000 fatal courses. in most cases this infection is a subclinical event with few or none symptoms noticeable for the patient. courses of this disease and incidence of this parasite in industrialised nations are not yet fully investigated. our case reports about a 68-year-old male patient from turkey who lives for more than 30 years in germany and had not been ... | 2013 | 24303471 |
| species-specific immunity induced by infection with entamoeba histolytica and entamoeba moshkovskii in mice. | entamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. there is currently no vaccine against this parasite. we have previously shown that intracecal inoculation of e. histolytica trophozoites leads to chronic and non-healing cecitis in mice. entamoeba moshkovskii, a closely related amoeba, also causes diarrhea and other intestinal disorders in this model. here, we investigated the effect of infection followed by drug-cure of these ... | 2013 | 24312397 |
| highly divergent mitochondrion-related organelles in anaerobic parasitic protozoa. | the mitochondria have arisen as a consequence of endosymbiosis of an ancestral α-proteobacterium with a methane-producing archae. the main function of the canonical aerobic mitochondria include atp generation via oxidative phosphorylation, heme and phospholipid synthesis, calcium homeostasis, programmed cell death, and the formation of iron-sulfur clusters. under oxygen-restricted conditions, the mitochondrion has often undergone remarkable reductive alterations of its content and function, lead ... | 2014 | 24316280 |
| comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard. | performance evaluation of diagnostic tests is critical in the search for accurate diagnoses. a gold standard test is usually absent in parasitology, thus rendering satisfactory assessment of diagnostic accuracy difficult. moreover, reliability (assessed by the study of repeatability) is a rarely studied characteristic of diagnostic tests. this study compared and evaluated the performance (repeatability, concordance and accuracy) of the spontaneous sedimentation technique (sst) and the paratest f ... | 2014 | 24321383 |
| multilocus sequence typing system (mlst) reveals a significant association of entamoeba histolytica genetic patterns with disease outcome. | the relationship between parasite genotypes and outcome of amoebic infection is still a paradox and needed to be explored. proper identification and genetic characterization of entamoeba histolytica clinical isolates is an effective tool for exploring this relation. along with conventional polymorphic marker (chitinase), trna linked short tandem repeat (str) loci has been employed as multilocus genotyping tool due to its better resolution and evolutionary significance. some common as well as exc ... | 2014 | 24321887 |
| synthesis, characterization, and anti-amoebic activity of n-(pyrimidin-2-yl)benzenesulfonamide derivatives. | a new series of n-(pyrimidin-2-yl)benzenesulfonamide derivatives, 3a-3i and 4a-4i, was synthesized from pyrimidin-2-amines, 2a-2i, with the aim to explore their effects on in vitro growth of entamoeba histolytica. the chemical structures of the compounds were elucidated by elemental analysis, ft-ir, (1) h- and (13) c-nmr, and esi mass-spectral data. in vitro anti-amoebic activity was evaluated against hm1 : imss strain of entamoeba histolytica. the ic50 values were calculated by using the double ... | 2013 | 24327446 |
| breast-feeding protects infantile diarrhea caused by intestinal protozoan infections. | this study investigated the effect of breast-feeding in protection against protozoan infection in infants with persistent diarrhea. infants were classified into 2 groups; 161 breast-fed infants and the same number of non-breast-fed infants. microscopic examinations of stool were done for detection of parasites and measuring the intensity of infection. moreover, serum levels of ige and tnf-α were measured by elisa. cryptosporidium spp., entamoeba histolytica/entamoeba dispar, giardia lamblia, and ... | 2013 | 24327776 |
| clinical significance of high anti-entamoeba histolytica antibody titer in asymptomatic hiv-1-infected individuals. | anti-entamoeba histolytica antibody (anti- e. histolytica) is widely used in seroprevalence studies though its clinical significance has not been assessed previously. | 2014 | 24338349 |
| evaluation of the effect of miltefosine on trichomonas vaginalis. | trichomonas vaginalis causes trichomoniasis in humans, a sexually transmitted disease commonly treated with metronidazole (mtz). mtz is known to cause undesirable side effects, and mtz-resistant parasites have been reported. thus, the development of an alternative treatment is desirable. miltefosine (mlt) is an alkylphosphocholine synthetic lipid analogue that displays antiparasitic activity against leishmania, trypanosoma cruzi, entamoeba histolytica, acanthamoeba spp., giardia lamblia, t. vagi ... | 2014 | 24363204 |
| rickettsiae, protozoa, and opisthokonta/metazoa. | rhizobiales (formerly named rickettsiales) cause in rare instances meningitis and meningovasculitis, respectively. in case of history of exposure, infection by rhizobiales needs to be considered since both diagnosis and therapy may be extremely difficult and pathogen-specific. the same applies to protozoa; in this chapter, babesia species, free-living amoebae and entamoeba histolytica infection, including severe meningitis and brain abscess, infection by trypanosoma species (south american and a ... | 2014 | 24365428 |
| prevalence of intestinal parasites in primary school children of mthatha, eastern cape province, south africa. | the presence of intestinal parasites in a population group is indicative of lack of proper sanitation, low economic standards and poor educational background. | 2013 | 24380000 |
| identification of the virulence landscape essential for entamoeba histolytica invasion of the human colon. | entamoeba histolytica is the pathogenic amoeba responsible for amoebiasis, an infectious disease targeting human tissues. amoebiasis arises when virulent trophozoites start to destroy the muco-epithelial barrier by first crossing the mucus, then killing host cells, triggering inflammation and subsequently causing dysentery. the main goal of this study was to analyse pathophysiology and gene expression changes related to virulent (i.e. hm1:imss) and non-virulent (i.e. rahman) strains when they ar ... | 2013 | 24385905 |
| snap-tag technology optimized for use in entamoeba histolytica. | entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. the pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. here we present the optimization of snap-tag technology via codon optimization specific for e. histolytica. the resultant snap protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. we ... | 2013 | 24391864 |
| an integrated microfluidic device for rapid serodiagnosis of amebiasis. | a microfluidic device was successfully fabricated for the rapid serodiagnosis of amebiasis. a micro bead-based immunoassay was fabricated within integrated microfluidic chip to detect the antibody to entamoeba histolytica in serum samples. in this assay, a recombinant fragment of c terminus of intermediate subunit of galactose and n-acetyl-d-galactosamine-inhibitable lectin of entamoeba histolytica (c-igl, aa 603-1088) has been utilized instead of the crude antigen. this device was validated wit ... | 2013 | 24403984 |
| intestinal protozoans in adults with diarrhea. | diarrhea is one of the most common presenting complaints in human immunodeficiency virus-infected individuals. | 2013 | 24404554 |
| assessment of parasitic pollution in the coastal seawater of gaza city. | the main objective of the study was the assessment of the prevalence and the identification of species of human gastrointestinal parasites as an indicator of the pollution of the seashore of gaza city. | 2014 | 24410999 |
| bioassay-guided fractionation of extracts from codiaeum variegatum against entamoeba histolytica discovers compounds that modify expression of ceramide biosynthesis related genes. | leaves of codiaeum variegatum ("garden croton") are used against bloody diarrhoea by local populations in cameroon. this study aims to search for the active components from c. variegatum against entamoeba histolytica, and thereby initiate the study of their mechanism of action. a bioassay-guided screening of the aqueous extracts from c. variegatum leaves and various fractions was carried out against trophozoites of e. histolytica axenic culture. we found that the anti-amoebic activity of extract ... | 2014 | 24416462 |
| nano-yeast-scfv probes on screen-printed gold electrodes for detection of entamoeba histolytica antigens in a biological matrix. | the time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. herein, we present low-cost affinity reagents, nano-yeast-scfv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free elec ... | 2014 | 24434498 |
| a genomewide overexpression screen identifies genes involved in the phosphatidylinositol 3-kinase pathway in the human protozoan parasite entamoeba histolytica. | entamoeba histolytica is a protozoan parasite that causes amoebic dysentery and liver abscess. e. histolytica relies on motility, phagocytosis, host cell adhesion, and proteolysis of extracellular matrix for virulence. in eukaryotic cells, these processes are mediated in part by phosphatidylinositol 3-kinase (pi3k) signaling. thus, pi3k may be critical for virulence. we utilized a functional genomics approach to identify genes whose products may operate in the pi3k pathway in e. histolytica. we ... | 2014 | 24442890 |
| new insights into host-pathogen interactions during entamoeba histolytica liver infection. | amoebiasis is the third worldwide disease due to a parasite. the causative agent of this disease, the unicellular eukaryote entamoeba histolytica, causes dysentery and liver abscesses associated with inflammation and human cell death. during liver invasion, before entering the parenchyma, e. histolytica trophozoites are in contact with liver sinusoidal endothelial cells (lsec). we present data characterizing human lsec responses to interaction with e. histolytica and identifying amoebic factors ... | 2011 | 24466432 |
| synthesis of 3-tetrazolylmethyl-4h-chromen-4-ones via ugi-azide and biological evaluation against entamoeba histolytica, giardia lamblia and trichomona vaginalis. | the synthesis of novel 3-tetrazolylmethyl-4h-chromen-4-ones via an ugi-azide multicomponent reaction and their biological evaluation against entamoeba histolytica, giardia lamblia and trichomona vaginalis are described. reported yields are moderate to good and biological results show that these compounds could be considered as candidates to anti-parasitic drugs, especially against g. lamblia. | 2014 | 24468633 |
| susceptibility to entamoeba histolytica intestinal infection is related to reduction in natural killer t-lymphocytes in c57bl/6 mice. | entamoeba histolytica is a protozoan that causes amoebiasis. recent studies demonstrated that natural killer t lymphocytes (nkt) are critical for preventing the development of amoebic liver abscess. in spite of that, there are only a handful of studies in the area. herein, we explored the role of nkt cells in e. histolytica infection using c57bl/6 wild-type and cd1(-/-) mice. animals were inoculated with e. histolytica and sacrificed 48 hours later to collect caecum samples that were used for qu ... | 2012 | 24470941 |
| stress granule formation in entamoeba histolytica: cross-talk between ehmlbp, ehrle3 reverse transcriptase and polyubiquitinated proteins. | the entamoeba histolytica-methylated line-binding protein (ehmlbp) binds to methylated repetitive dna and is a positive regulator of a reverse transcriptase of a long interspersed nucleotide element (line). this protein protects trophozoites against heat shock by reducing protein aggregation. the presence of ehmlbp and polyubiquitinated proteins in heat shock-induced protein aggregates raised the question whether these proteins interact. this assumption was confirmed by co-immunoprecipitation ex ... | 2014 | 24471581 |
| insights into the gtp/gdp cycle of rabx3, a novel gtpase from entamoeba histolytica with tandem g-domains. | members of the small gtpase ras superfamily regulate a host of systems through their ability to catalyze the gtp/gdp cycle. all family members reported thus far possess a single gtpase domain with a p-loop containing a nucleoside triphosphate hydrolase fold. here for the first time we report a novel member from entamoeba histolytica, ehrabx3, which harbors two gtpase domains in tandem and exhibits unique biochemical properties. a combination of biochemical and microcalorimetric studies revealed ... | 2014 | 24471929 |
| a novel c-terminal homologue of aha1 co-chaperone binds to heat shock protein 90 and stimulates its atpase activity in entamoeba histolytica. | cytosolic heat shock protein 90 (hsp90) has been shown to be essential for many infectious pathogens and is considered a potential target for drug development. in this study, we have carried out biochemical characterization of hsp90 from a poorly studied protozoan parasite of clinical importance, entamoeba histolytica. we have shown that entamoeba hsp90 can bind to both atp and its pharmacological inhibitor, 17-aag (17-allylamino-17-demethoxygeldanamycin), with kd values of 365.2 and 10.77 μm, r ... | 2014 | 24486610 |
| association between allelic variation due to short tandem repeats in trna gene of entamoeba histolytica and clinical phenotypes of amoebiasis. | genotypes of entamoeba histolytica (e. histolytica) may contribute clinical phenotypes of amoebiasis such as amoebic liver abscess (ala), dysentery and asymptomatic cyst passers state. hence, we evaluated allelic variation due to short tandem repeats (strs) in trna gene of e. histolytica and clinical phenotypes of amoebiasis. asymptomatic cyst passers (n=24), patients with dysentery (n=56) and ala (n=107) were included. extracted dna from stool (dysentery, asymptomatic cyst passers) and liver as ... | 2014 | 24495629 |
| [detection of entamoeba histolytica and entamoeba dispar by pcr in children, less than five years of age with diarrhea, in maracaibo, venezuela. a preliminary study]. | to determine the prevalence of entamoeba histolytica as a producer of diarrhea, a study was conducted in children, less than five years of age, with diarrhea who attended several out patient clinics of the servicio aut6nomo hospital universitario, maracaibo, venezuela. a macroscopic and microscopic examination with physiological saline, lugol and kinyoun staining were performed to the stool samples obtained. the remainder of the sample was frozen until dna extraction, and pcr amplification was p ... | 2013 | 24502179 |
| structure, absolute configuration, and antidiarrheal activity of a thymol derivative from ageratina cylindrica. | the leaves of ageratina cylindrica afforded a thymol derivative that was characterized by physical and spectroscopical methods as (8s)-8,9-epoxy-6-hydroxy-l0-benzoyloxy-7-oxothymol isobutyrate (1). the absolute configuration of 1 was established as 8s by vibrational circular dichroism spectroscopy in combination with density functional theory calculations and by evaluation of the flack and hooft x-ray parameters. compound 1 showed weak antiprotozoal activity against entamoeba histolytica and gia ... | 2014 | 24502360 |
| entamoeba histolytica-secreted cysteine proteases induce il-8 production in human mast cells via a par2-independent mechanism. | entamoeba histolytica is an extracellular tissue parasite causing colitis and occasional liver abscess in humans. e. histolytica-derived secretory products (sps) contain large amounts of cysteine proteases (cps), one of the important amoebic virulence factors. although tissue-residing mast cells play an important role in the mucosal inflammatory response to this pathogen, it is not known whether the sps induce mast cell activation. in this study, when human mast cells (hmc-1 cells) were stimulat ... | 2014 | 24502918 |
| the future for vaccine development against entamoeba histolytica. | entamoeba histolytica is the causative agent of amebiasis, one of the top three parasitic causes of mortality worldwide. in the majority of infected individuals, e. histolytica asymptomatically colonizes the large intestine, while in others, the parasite breaches the mucosal epithelial barrier to cause amebic colitis and can disseminate to soft organs to cause abscesses. vaccinations using native and recombinant forms of the parasite gal-lectin have been successful in protecting animals against ... | 2014 | 24504133 |
| [comparison of direct microscopy, culture, elisa and molecular methods for diagnosis of entamoeba histolytica]. | amebiasis, a parasitic infection caused by entamoeba histolytica, is one of the most common parasitic infections worldwide. since it is still an important public health problem in developing countries, rapid differential diagnosis of amebiasis is crucial in terms of treatment. the most frequently used method for laboratory diagnosis is direct microscopy, however more reliable and specific methods are needed in order to differentiate the apathogenic entamoeba dispar under the microscope. this stu ... | 2014 | 24506721 |
| a cross-sectional study on intestinal parasitic infections in rural communities, northeast thailand. | despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in southeast asia, including thailand. in rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. this study presents a survey of the prevalence of intestinal parasitic infections among the people in rural thailand. the community-based cross-sectional study was conducted in villages in khon ... | 2013 | 24516280 |
| prevalence of intestinal parasitic infections and associated risk factors among kigali institute of education students in kigali, rwanda. | intestinal parasitic infections (ipis) are a significant public health problem in sub- saharan africa (ssa) and rwanda is not spared. while eradication programs towards preschool-aged and school-aged children are undertaken, important gaps regarding ipis among students attending tertiary learning institutions remain. the aim of this study was to determine the prevalence of ipis and associated risk factors among kigali institute of education (kie) students who attended its medical clinic for stoo ... | 2013 | 24522143 |
| design, synthesis and biological evaluation of 3-[4-(7-chloro-quinolin-4-yl)-piperazin-1-yl]-propionic acid hydrazones as antiprotozoal agents. | n-acylhydrazones derived from 7-chloro-4-piperazin-1-yl-quinoline were synthesized and biologically evaluated for blood-stage of plasmodium falciparum and entamoeba histolytica trophozoites. n-acylhydrazone f12 was found to inhibit the p. falciparum growth as well as its life cycle with good selectivity, which was achieved by inhibiting hematin formation. compound f24 showed better ic50 value than the amoebicidal drug metronidazole. | 2014 | 24530492 |
| nitroimidazole drugs vary in their mode of action in the human parasite giardia lamblia. | giardia lamblia (syn. duodenalis, intestinalis) is a globally occurring micro-aerophilic human parasite that causes gastrointestinal disease. standard treatment of g. lamblia infections is based on the 5-nitroimidazole drugs metronidazole and tinidazole. in two other micro-aerophilic parasites, entamoeba histolytica and trichomonas vaginalis, 5-nitroimidazole drugs bind to proteins involved in the thioredoxin-mediated redox network and disrupt the redox equilibrium by inhibiting thioredoxin redu ... | 2012 | 24533278 |
| synthesis of 4'-(2-ferrocenyl)-2,2':6'2''-terpyridine: characterization and antiprotozoal activity of mn(ii), co(ii), ni(ii), cu(ii) and zn(ii) complexes. | a terpyridine ligand fctpy was reacted with divalent metals (cu, co, mn, ni and zn), yielding five complexes of general formula [metal(fctpy)2][pf6]2. the structure of fctpy was determined by single crystal x-ray diffraction studies. the complexes characterized using various spectroscopic techniques suggested an octahedral geometry around the central metal ion. these complexes were screened for their antiamoebic, trypanocidal and antimalarial activities. it was found that, complexes 2 and 3 show ... | 2014 | 24534536 |
| expression of ehrad54, ehrad51, and ehblm proteins during dna repair by homologous recombination in entamoeba histolytica. | entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events. it contains the rad52 epistasis group genes, including ehrad51 and ehrad54, and the ehblm gene, which are key homologous recombination factors in other organisms. ehrad51 and ehrad54 genes are differentially transcribed in trophozoites when dna double-strand breaks are induced by ultraviolet-c irradiation. moreover, the ehrad51 rec ... | 2014 | 24534563 |
| unusual multiple large abscesses of the liver: interest of the radiological features and the real-time pcr to distinguish between bacterial and amebic etiologies. | we report a rare case of amebiasis generating 19 large liver abscesses. such a quantity of abscesses is rare, especially when occurring in a young casual traveler without any immunodeficiency disorders. a possible co-infection was excluded. by contrast, the amebic etiology was confirmed by means of serology and real-time pcr. | 2014 | 24548161 |
| crystal structure and putative substrate identification for the entamoeba histolytica low molecular weight tyrosine phosphatase. | entamoeba histolytica is a eukaryotic intestinal parasite of humans, and is endemic in developing countries. we have characterized the e. histolytica putative low molecular weight protein tyrosine phosphatase (lmw-ptp). the structure for this amebic tyrosine phosphatase was solved, showing the ligand-induced conformational changes necessary for binding of substrate. in amebae, it was expressed at low but detectable levels as detected by immunoprecipitation followed by immunoblotting. a mutant lm ... | 2014 | 24548880 |
| frequency of amoebiasis and other intestinal parasitoses in a settlement in ilhéus city, state of bahia, brazil. | this study evaluated the frequency of intestinal parasites, emphasizing the identification and differentiation of entamoeba spp. | 2014 | 24553799 |
| risk factors for intestinal parasitosis among antiretroviral-treated hiv/aids patients in ethiopia. | summary a cross-sectional survey was conducted to determine the risk factors associated with intestinal parasitosis in hiv/aids patients receiving antiretroviral therapy (art). socio-demographic information was collected and faecal samples were analysed from 384 randomly selected patients on art. data on cd4+ t-cell counts and world health organization clinical staging were obtained from the medical records at the hospital. the overall prevalence of intestinal parasitosis was 56% (95% confidence ... | 2014 | 24554001 |
| the entamoeba histolytica dnmt2 homolog (ehmeth) confers resistance to nitrosative stress. | nitric oxide (no) has antimicrobial properties against many pathogens due to its reactivity as an s-nitrosylating agent. it inhibits many of the key enzymes that are involved in the metabolism and virulence of the parasite entamoeba histolytica through s-nitrosylation of essential cysteine residues. very little information is available on the mechanism of resistance to no by pathogens in general and by this parasite in particular. here, we report that exposure of the parasites to s-nitrosoglutat ... | 2014 | 24562908 |
| comparative study of the prevalence of intestinal parasites in low socioeconomic areas from south chennai, india. | intestinal parasites cause one of the most important health problems through their effects in causing undernourishment morbidity and incapacitation due to their behavior particularly in children compared to adults. this study was intended to state the prevalence of intestinal parasites between the slum dwellers of different areas in south chennai. among the total of 256 samples collected between the ages of 0-50 yrs, 194 samples were positive. standard laboratory techniques for parasitological d ... | 2014 | 24587897 |
| longitudinal evaluation of enteric protozoa in haitian children by stool exam and multiplex serologic assay. | haitian children were monitored longitudinally in a filariasis study. included were stool samples examined for giardia intestinalis and entamoeba histolytica cysts, and serum specimens analyzed for immunoglobulin g (igg) responses to eight recombinant antigens from g. intestinalis (variant-specific surface protein [vsp1-vsp5]), e. histolytica (lectin adhesion molecule [leca]), and cryptosporidium parvum (17- and 27-kda) using a multiplex bead assay. the igg responses to vsp antigens peaked at 2 ... | 2014 | 24591430 |
| laboratory diagnosis of amebiasis in a sample of students from southeastern brazil and a comparison of microscopy with enzyme-linked immunosorbent assay for screening of infections with entamoeba sp. | epidemiological studies on amebiasis have been reassessed since entamoeba histolytica and e. dispar were first recognized as distinct species. because the morphological similarity of these species renders microscopic diagnosis unreliable, additional tools are required to discriminate between entamoeba species. the objectives of our study were to compare microscopy with elisa kit (ivd®) results, to diagnose e. histolytica infection, and to determine the prevalence of amebiasis in a sample of stud ... | 2014 | 24603737 |
| entamoeba histolytica: gene expression analysis of cells invading tissues. | entamoeba histolytica is a protozoan parasite that presents a risk to the health of millions of people worldwide. due to the existence of different clinical forms caused by the parasite and also different virulence levels presented by one strain, one would expect differences in the profile of gene transcripts between virulent and nonvirulent cultures. in this study we used the differential display to select gene segments related to invasiveness of amoeba. one brazilian strain of e. histolytica i ... | 2014 | 24605052 |