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bacillus cereus iron uptake protein fishes out an unstable ferric citrate trimer.citrate is a common biomolecule that chelates fe(iii). many bacteria and plants use ferric citrate to fulfill their nutritional requirement for iron. only the escherichia coli ferric citrate outer-membrane transport protein feca has been characterized; little is known about other ferric citrate-binding proteins. here we report a unique siderophore-binding protein from the gram-positive pathogenic bacterium bacillus cereus that binds multinuclear ferric citrate complexes. we have demonstrated tha ...201223027976
synthesis of bacillithiol and the catalytic selectivity of fosb-type fosfomycin resistance proteins.bacillithiol (bsh) has been prepared on the gram scale from the inexpensive starting material, d-glucosamine hydrochloride, in 11 steps and 8-9% overall yield. the bsh was used to survey the substrate and metal-ion selectivity of fosb enzymes from four gram-positive microorganisms associated with the deactivation of the antibiotic fosfomycin. the in vitro results indicate that the preferred thiol substrate and metal ion for the fosb from staphylococcus aureus are bsh and ni(ii), respectively. ho ...201223030527
inactivation of bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit. 201223034184
the methylomes of six bacteria.six bacterial genomes, geobacter metallireducens gs-15, chromohalobacter salexigens, vibrio breoganii 1c-10, bacillus cereus atcc 10987, campylobacter jejuni subsp. jejuni 81-176 and c. jejuni nctc 11168, all of which had previously been sequenced using other platforms were re-sequenced using single-molecule, real-time (smrt) sequencing specifically to analyze their methylomes. in every case a number of new n(6)-methyladenine ((m6)a) and n(4)-methylcytosine ((m4)c) methylation patterns were disc ...201223034806
sr1--a small rna with two remarkably conserved functions.sr1 is a dual-function srna that acts as a base-pairing regulatory rna on the ahrc mrna and as a peptide-encoding mrna on the gapa operon. the sr1-encoded peptide sr1p binds gapa thereby stabilizing gapa mrna. under glycolytic conditions, sr1 transcription is repressed by ccpn and ccpa. a computer-based search identified 23 sr1 homologues in bacillus, geobacillus, anoxybacillus and brevibacillus species. all homologues share a high structural identity with bacillus subtilis sr1, and the encoded ...201223034808
technological properties and probiotic potential of enterococcus faecium strains isolated from cow milk.to identify enterococci from the fermentation of milk for the production of nono, an african fermented dairy product, to determine the technological properties for suitability as starter cultures and safety as probiotics.201323035976
antimicrobial activity and composition profile of grape (vitis vinifera) pomace extracts obtained by supercritical fluids.the possibility of increasing the aggregated value of the huge amount of residues generated by wineries around the world foment studies using the grape pomace - the residue from the wine production, composed by seed, skin and stems - to obtain functional ingredients. nowadays, consumers in general prefer natural and safe products mainly for food and cosmetic fields, where the supercritical fluid extraction is of great importance due to the purity of the extracts provided. therefore, the objectiv ...201323036924
a case series of bacillus cereus septicemia in patients with hematological disease.bacillus cereus (b. cereus) septicemia is a cause of life-threatening infection in patients with hematologic diseases. however, preventing a fatal prognosis in patients with b. cereus infection has not yet been achieved due to insufficient clinical investigations. to discover more optimal treatment strategies, we analyzed b. cereus septicemia in patients with hematologic diseases.201223037464
leukemic manifestation of blastic plasmacytoid dendritic cell neoplasm lacking skin lesion : a borderline case between acute monocytic leukemia.blastic plasmacytoid dendritic cell neoplasm (bpdcn) is a rare hematologic malignancy with a poor prognosis. we encountered a unique case of bpdcn that was leukemic at presentation without skin lesion and expressed cd33 antigen. a 74-year-old man was admitted because of dyspnea. physically, hepatosplenomegaly, but not skin lesions and superficial lymph node swelling, was noted. the white blood count was 33.6 × 10(9)/l with 19% giant abnormal cells. these cells were positive for cd4, cd86, cd123 ...201223037626
changes in microbial contamination levels and prevalence of foodborne pathogens in alfalfa (medicago sativa) and rapeseed (brassica napus) during sprout production in manufacturing plants.samples were taken from three sprout processing plants at five different stages of production (a total of 20 investigations). quantitative analyses comprised aerobic plate counts (apcs) and the measurement of coliforms and bacillus cereus levels, whereas qualitative analyses involved assessing the levels of escherichia coli and major foodborne pathogens (e. coli o157:h7, listeria monocytogenes, salmonella spp., and staphylococcus aureus). the apc for alfalfa seeds (3·71-4·61 log cfu g(-1)) and r ...201323051104
evaluation of a potential starter culture for enhance quality of coffee fermentation.the coffee fermentation is characterized by the presence of different microorganisms belonging to the groups of bacteria, fungi and yeast. the objectives of this work were to select pectinolytic microorganisms isolated from coffee fermentations and evaluate their performance on coffee pulp culture medium. the yeasts and bacteria isolates were evaluated for their activity of polygalacturonase (pg), pectin lyase (pl) and pectin methylesterase (pme) and metabolites production. among 127 yeasts isol ...201323054699
can bacillus cereus food poisoning cause sudden death? 201223066123
[evaluation of multiplex pcr to identify the species of microorganisms from bacillus cereus group].bacillus genus comprises about 215 species. the most closely related are b. cereus, b. thuringiensis, b. anthracis, b. mycoides, b. pseudomycoides and b. weihenstephanensis. these bacterial species belong to the bacillus cereus group. identification and differentiation of bacillus cereus group bacteria is difficult because of genetic and phenotypic similarity. many molecular methods have already been suggested to differentiate b. cereus group members. however easier and more convenient methods a ...201223072054
multilocus sequence analysis of bacillus thuringiensis serovars navarrensis, bolivia and vazensis and bacillus weihenstephanensis reveals a common phylogeny.the bacillus cereus group sensu lato includes six closely-related bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides and bacillus weihenstephanensis. b. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. b. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16s rrna gene and cspa genes. a ...201323073664
chemical composition and antimicrobial activity of salvia x jamensis essential oil.this is the first report about the composition of the essential oil of salvia x jamensis. the major compounds identified by gc-ms were beta-caryophyllene (14.8 %), beta-pinene (6.8 %), caryophyllene oxide (6.0 %), delta-cadinene (5.5 %), alpha-pinene (5.2 %) and spatulenol (5.2 %). the oil showed antimicrobial activity against three gram (+) bacteria: bacillus cereus, staphylococcus aureus, and s. epidermidis, and three candida strains: c. albicans, c. glabrata and c. tropicalis, while it was in ...201223074919
postprocessing microflora of commercial attieke (a fermented cassava product) produced in the south of côte d'ivoire.the distribution and presence of hygiene indicator and pathogenic micro-organisms in 375 samples of attieke marketed in côte d'ivoire, and their roles in the food poisoning were evaluated. microbiological analyses were carried out, which included the total viable bacteria, coliforms, escherichia coli, staphylococcus aureus, salmonella, bacillus spores, fungi and clostridium perfringens. the results revealed that the viable bacteria counts ranged from 2·2 ± 1·2 × 10(5) to 3·4 ± 1·4 × 10(6) cfu g( ...201323078634
production and purification of a protease, a chitosanase, and chitin oligosaccharides by bacillus cereus tku022 fermentation.a protease- and chitosanase-producing strain was isolated and identified as bacillus cereus tku022. the protease and chitosanase were both produced using 1.5% (w/v) shrimp head powder (shp) as the sole carbon/nitrogen source, and these enzymes were purified from the culture supernatant. the molecular masses of the tku022 protease and chitosanase determined using sds-page were approximately 45 and 44kda, respectively. the high stability of the tku022 protease toward surfactants, an optimal ph of ...201223079238
mass spectrometric profiling of bacillus cereus strains and quantitation of the emetic toxin cereulide by means of stable isotope dilution analysis and hep-2 bioassay.a fast and robust high-throughput ultra-performance liquid chromatography/time-of-flight mass spectrometry (uplc-tof ms) profiling method was developed and successfully applied to discriminate a total of 78 bacillus cereus strains into no/low, medium and high producers of the emetic toxin cereulide. the data obtained by uplc-tof ms profiling were confirmed by absolute quantitation of cereulide in selected samples by means of high-performance liquid chromatography with tandem mass spectrometry (h ...201323079954
early murine immune responses from endotracheal exposures to biotechnology-related bacillus strains.an immunology-based in vivo screening regime was used to assess the potential pathogenicity of biotechnology-related microbes. strains of bacillus cereus (bc), bacillus subtilis (bs), bacillus thuringiensis (bt), and bt commercial products (cps) were tested. balb/c mice were endotracheally instilled with purified spores, diluted cp, or vegetative cells (vc) (live or dead). exposed mice were evaluated for changes in behavioral and physical symptoms, bacterial clearance, pulmonary granulocytes, an ...201123087536
divergence of the sigb regulon and pathogenesis of the bacillus cereus sensu lato group.the bacillus cereus sensu lato group currently includes seven species (b. cereus, b. anthracis, b. mycoides, b. pseudomycoides, b. thuringiensis, b. weihenstephanensis and b. cytotoxicus) that recent phylogenetic and phylogenomic analyses suggest are likely a single species, despite their varied phenotypes. although horizontal gene transfer and insertion-deletion events are clearly important for promoting divergence among these genomes, recent studies have demonstrated that a major basis for phe ...201223088190
combined effect of cadmium, lead, and uv rays on bacillus cereus using comet assay and oxidative stress parameters.exposure to environmental chemicals and oxidative stress particularly at low dose levels may produce additive or synergistic interactions not seen in single component exposure. exposure to cadmium, lead, and ultraviolet rays occurs in many occupational settings, such as pigment and battery production, galvanization, and recycling of electric tools. however, little is known about interactions between heavy metals and ultraviolet rays. this study aimed to evaluate the interactions of ultraviolet r ...201523089956
biosorption of arsenic (iii) from aqueous solution by living cells of bacillus cereus.in this work, removal of arsenic (iii) from aqueous solution by living cells (bacillus cereus), biosorption mechanism, and characterization studies have been reported. b. cereus cell surface was characterized using sem-edx and ftir. dependence of biosorption on ph of the solution, biosorbent dose, initial arsenic (iii) concentration, contact time, and temperature had been studied to achieve optimum condition. the maximum biosorption capacity of living cells of b. cereus for arsenic (iii) was fou ...201323093415
drosophila melanogaster selection for survival of bacillus cereus infection: life history trait indirect responses.to study evolved resistance/tolerance in an insect model, we carried out an experimental evolution study using d. melanogaster and the opportunistic pathogen b. cereus as the agent of selection. the selected lines evolved a 3.0- to 3.3-log increase in the concentration of spores required for 50% mortality after 18-24 generations of selection. in the absence of any treatment, selected lines evolved an increase in egg production and delayed development time. the latter response could be interprete ...201223094195
synthesis and characterization of a series of transition metal complexes with a new symmetrical polyoxaaza macroacyclic schiff base ligand: x-ray crystal structure of cobalt(ii) and nickel(ii) complexes and their antibacterial properties.a new symmetrical [n4o2] hexadentate schiff base ligand, (e)-n-(pyridin-2-ylmethylene)-2-(3-(2-((e)-pyridin-2-lmethyleneamino)phenoxy)naphthalen-2-yloxy)benzenamine, abbreviated to l, and its complexes of ni(ii), cu(ii), zn(ii), co(ii), cd(ii) and mn(ii) have been synthesized in the presence of metal ions. the complexes were structurally characterized by elemental analyses, ir, uv-vis, nmr and molar conductivity. the crystal structures of two complexes, [nil(ono2)2]·2h2o and [colcl2]ch3oh·0.5h2o ...201323099161
in vitro evaluation of datura innoxia (thorn-apple) for potential antibacterial activity.various parts of datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against gram-negative bacteria (escherichia coli and salmonella typhi) and gram-positive bacteria (bacillus cereus, bacillus subtilis and staphylococcus aureus). the results of agar well diffusion assay indicated that the pattern of inhibition depends largely upon the plant part, solvent used for extraction and the organism tested. extracts prepared from leaves ...200823100734
simultaneous detection of pathogenic b. cereus, s. aureus and l. monocytogenes by multiplex pcr.three important foodborne pathogens, bacillus cereus, listeria monocytogenes and staphylococcus aureus are of major concern for food safety in terms of frequency and seriousness of the disease. the occurrence these three important pathogens and their coexistence in food matrices are predominant. moreover, symptoms associated with b. cereus and s. aureus food poisoning not only closely resembles each other but can also be overlapping with other foodborne infections. in this context, detection of ...200923100783
multiplex pcr assay for the detection of enterotoxic bacillus cereus group strains and its application in food matrices.bacillus cereus, bacillus thuringiensis and bacillus anthracis are the major concerns for the food safety in terms of frequency and/or seriousness of the disease. being members of the same group and sharing dna homology to a larger extent, they do create problems when their specific detection/identification is attempted from different food and environmental sources. numerous individual polymerase chain reaction (pcr) and few multiplex pcr (mpcr) methods have been employed to detect these organis ...201023100823
chemical composition and antioxidative activity of echinophora platyloba dc. essential oil, and its interaction with natural antimicrobials against food-borne pathogens and spoilage organisms.this study was undertaken to determine the chemical composition and antioxidative capacity of echinophora platyloba dc. essential oil, and its antimicrobial potency against listeria monocytogenes, bacillus cereus, bacillus subtilis, staphylococcus aureus, salmonella typhimurium, escherichia coli o157:h7, pseudomonas aeruginosa, candida albicans, candida tropicalis, rhodotorula rubra, and rhodotorula mucilaginosa. the essential oil was analyzed by gc and gc-ms; and evaluated for its antioxidative ...201223106123
rapid detection and respirometric profiling of aerobic bacteria on panels of selective media.to evaluate high-throughput optical oxygen microrespirometry for selective detection and predictive identification of aerobic bacteria.201323107004
antibacterial effects of ellagitannins from acalypha wilkesiana var. macafeana hort.: surface morphology analysis with environmental scanning electron microscopy and synergy with antibiotics.the present study served to gain further insight into the bactericidal effects of ellagitannins from acalypha wilkesiana var. macafeana hort. against pathogenic bacteria. ellagitannins from the aerial parts of a. wilkesiana var. macafeana hort. (eaw) inhibited the growth of bacillus cereus (atcc 11778), bacillus subtilis (atcc 6633), staphylococcus aureus (atcc 11632) and methicillin-resistant staphylococcus aureus (mrsa) clinical strain with inhibition zones equal to 11.01 ± 1.53 mm, 16.63 ± 0. ...201323109276
immunization of mice with formalin-inactivated spores from avirulent bacillus cereus strains provides significant protection from challenge with bacillus anthracis ames.bacillus anthracis spores are the infectious form of the organism for humans and animals. however, the approved human vaccine in the united states is derived from a vegetative culture filtrate of a toxigenic, nonencapsulated b. anthracis strain that primarily contains protective antigen (pa). immunization of mice with purified spore proteins and formalin-inactivated spores (fis) from a nonencapsulated, nontoxigenic b. anthracis strain confers protection against b. anthracis challenge when pa is ...201323114705
[clinical case of the month. acute post-traumatic panophthalmitis].panophtalmitis is an intra-ocular and intra-orbital rare infection, but its consequences are often horrendous, with loss of both function and anatomy of the eye. the surgical approach and the antibiotherapy should be given as soon as possible, both by intra-vitreal and intra-venous injections. we report the case of a 49-year-old man who developed a bacillus cereus panophtalmitis. a delay in the diagnosis lead to the loss of vision and the evisceration of the sick eye. bacillus cereus panopthalmi ...201223115844
bacillus cereus ar156 induces resistance against rhizopus rot through priming of defense responses in peach fruit.the biocontrol effects of bacillus cereus ar156 on rhizopus rot caused by rhizopus stolonifer in postharvest peach fruit and the possible mechanisms were investigated. the results showed that fruit treated with b. cereus ar156 had significantly lower disease incidence and smaller lesion diameter than the control fruit did. b. cereus ar156 treatment remarkably enhanced activities of chitinase and β-1,3-glucanase, promoted accumulation of h(2)o(2), and improved total phenolic content and 2,2-diphe ...201323122077
synthesis of quinoline derivatives of tetrahydrocurcumin and zingerone and evaluation of their antioxidant and antibacterial attributes.tetrahydrocurcumin (thc, 1) and zingerone (2) are biologically active molecules originating from the important spices turmeric and ginger, respectively. novel quinoline derivatives of thc and zingerone have been synthesised by an efficient protocol involving their reaction with substituted 2-aminobenzophenones and 2-aminoacetophenone. radical-scavenging activities (rsa) of thc, zingerone and their quinoline derivatives were evaluated. the amino-substituted quinoline derivative of thc, 1e, showed ...201323122110
variation of cardinal growth parameters and growth limits according to phylogenetic affiliation in the bacillus cereus group. consequences for risk assessment.the growth rates of strains covering the seven major phylogenetic groups of bacillus cereus sensu lato (as defined by guinebretiere et al., 2008) at a range of temperature (7 °c-55 °c), ph (4.6-7.5) and a(w) (0.929-0.996, with 0.5%-10% nacl as humectant) were determined. growth rates were fitted by non-linear regression to determine the cardinal parameters t(min), t(opt), t(max), ph(min), ph(opt), a(wmin) and μ(opt). we showed that cardinal parameters reflected the differences in the temperature ...201323122503
bacterial quorum sensing: its role in virulence and possibilities for its control.quorum sensing is a process of cell-cell communication that allows bacteria to share information about cell density and adjust gene expression accordingly. this process enables bacteria to express energetically expensive processes as a collective only when the impact of those processes on the environment or on a host will be maximized. among the many traits controlled by quorum sensing is the expression of virulence factors by pathogenic bacteria. here we review the quorum-sensing circuits of st ...201223125205
heterologously expressed β-hydroxyl fatty acids from a metagenomic library of a marine sponge.functional screening based on the antibacterial activity of a metagenomic library of the japanese marine sponge, discodermia calyx, afforded three β-hydroxyl fatty acids: 3-hydroxypalmitic acid, 3-hydroxylauric acid and 3-hydroxymyristic acid, heterologously expressed in an antibacterial clone, pdc113. 3-hydroxypalmitic acid showed moderate antibacterial activity against bacillus cereus and candida albicans. a sequence analysis of the insert dna revealed 23 putative orfs, with most sharing homol ...201223127887
activation of bacillus spores at moderately elevated temperatures (30-33 °c).the time/temperature profiles experienced by spores on the track from their natural sporulation environment to consumable food products may be highly diverse. temperature has been documented as an important factor that may activate spores, i.e. potentiates spores to germinate. there is, however, limited knowledge about the relationship between the expected temperature history and the subsequent germination characteristics of bacterial spores. we show here that the germination rate of five differ ...201323132276
pathogenic potential of bacillus cereus strains as revealed by phenotypic analysis.the bacillus cereus pathogenic spectrum ranges from strains used as probiotics to human-lethal strains. however, prediction of the pathogenic potential of a strain remains difficult. here, we show that food poisoning and clinical strains can be differentiated from harmless strains on the basis of host colonization phenotypes.201323135929
a nine-coordinated bismuth(iii) complex derived from pentadentate 2,6-diacetylpyridine bis((4)n-methylthiosemicarbazone): crystal structure and both in vitro and in vivo biological evaluation.up to now, bismuth(iii) complexes with thiosemicarbazones have been comparatively rare. few in vivo biological studies have been carried out in comparison to the plentiful in vitro data. here, an interesting nine-coordinated bismuth(iii) complex, [bi(h2l)(no3)2]no3 [1; h2l = 2,6-diacetylpyridine bis((4)n-methylthiosemicarbazone)], has been synthesized and structurally characterized. the analytical data reveal the formation of 1:1 (metal/ligand) stoichiometry. in vitro biological studies have ind ...201223136979
genome sequence of the aerobic bacterium bacillus sp. strain fjat-13831.bacillus sp. strain fjat-13831 was isolated from the no. 1 pit soil of emperor qin's terracotta warriors in xi'an city, people's republic of china. the isolate showed a close relationship to the bacillus cereus group. the draft genome sequence of bacillus sp. fjat-13831 was 4,425,198 bp in size and consisted of 5,567 genes (protein-coding sequences [cds]) with an average length of 782 bp and a g+c value of 36.36%.201223144388
design, structural and spectroscopic elucidation, and the in vitro biological activities of new diorganotin dithiocarbamates.the reaction of 2,2-dimethoxy-n-methylethyllamine or 2-methyl-1,3-dioxolane with cs(2) in alkaline media produced two novel dithiocarbamate salts. subsequent reactions with organotin halides yielded six new complexes: [snme(2){s(2)cnr(r(1))(2)}(2)] (1), [sn(n-bu)(2){s(2)cnr(r(1))(2)}(2)] (2), [snph(2){s(2)cnr(r(1))(2)}(2)] (3), [snme(2){s(2)cnr(r(2))(2)}(2)] (4), [sn(n-bu)(2){s(2)cnr(r(2))(2)}(2)] (5), [snph(2){s(2)cnr(r(2))(2)}(2)] (6), where r = methyl, r(1) = ch(2)ch(ome)(2), and r(2) = 2-met ...201223159807
complementary screening, identification and application of a novel class ii 5-enopyruvylshikimate-3-phosphate synthase from bacillus cereus.a novel aroa gene encoding 5-enolpyruvylshikimate-3-phosphate synthase from bacillus cereus was identified and overexpressed by genomic library construction and complementary screening. the enzyme was then purified to homogeneity. we also transformed the aroa ( b. cereus ) gene into arabidopsis thaliana by a floral dip method, and demonstrated that transgenic a. thaliana plants exhibited significant glyphosate resistance compared with the wild type. these results strongly suggested that the stra ...201323161452
antagonistic effects of bacillus cereus strain b-02 on morphology, ultrastructure and cytophysiology of botrytis cinerea.the study on antagonistic mechanism of biocontrol strains gives the premise and basis for efficient and stable biological control. this study aimes to overcome of biocontrol agent in aspects of complicated and diversified mode of action, short-lasting and unstable efficacy in the production processes. this study elucidated the antagonistic mechanism of bacillus cereus strain b-02 on botrytis cinerea by detecting changes in morphology, ultrastructure and physiology in affected hyphae of botrytis ...201223163211
probiotic potential of lactobacillus spp. isolated from brazilian regional ovine cheese.twelve lactobacillus isolates from brazilian starter-free ovine cheeses were evaluated for their probiotic potential. the strains were identified by 16s rdna sequencing as lactobacillus plantarum (7), lb. brevis (2), lb. casei (2) and lb. parabuchneri (1). all strains showed variable resistance to gastric juices and relative tolerance to pancreatin and bile salts. only five strains of lb. plantarum could not deconjugate the sodium salt of taurodeoxycholic acid. autoaggregation ability after 24 h ...201223171587
germination inhibition of bacillus cereus spores: impact of the lipophilic character of inhibiting compounds.in this study, the impact of a range of organic acids and structurally similar alcohols with three to six carbon backbones and increasing lipophilic character, were tested on the germination behavior of b. cereus atcc 14579 spores. this approach allowed substantiating whether the effectivity of the various compounds was largely dictated by membrane interference or a classic weak acid acidification effect. the octanol-water partition coefficient (log p(oct/water)) ranges from 0.25/0.33 to 2.03/1. ...201223177052
antibacterial, antifungal and antioxidant activities of the ethanol extract of the stem bark of clausena heptaphylla.there is wide spread interest in drugs derived from plants as green medicine is believed to be safe and dependable, compared with costly synthetic drugs that have adverse effects.201223181593
role of the moth fly clogmia albipunctata (diptera: psychodinae) as a mechanical vector of bacterial pathogens in german hospitals.the formerly mediterranean moth fly species clogmia albipunctata (diptera: psychodidae) is now present in germany, where it has become a common, year-round pest in hospital buildings.201323182212
perhydrolase-nanotube paint composites with sporicidal and antiviral activity.act (perhydrolase) containing paint composites were prepared leading to broad-spectrum decontamination. act was immobilized onto multi-walled carbon nanotubes (mwnts) and then incorporated into latex-based paints to form catalytic coatings. these act-based paint composites showed a 6-log reduction in the viability of spores of bacillus cereus and bacillus anthracis (sterne) within 60 min. the paint composites also showed >4-log reduction in the titer of influenza virus (x-31) within 10 min (init ...201323188457
crystal structures of two transcriptional regulators from bacillus cereus define the conserved structural features of a padr subfamily.padr-like transcriptional regulators form a structurally-related family of proteins that control the expression of genes associated with detoxification, virulence and multi-drug resistance in bacteria. only a few members of this family have been studied by genetic, biochemical and biophysical methods, and their structure/function relationships are still largely undefined. here, we report the crystal structures of two padr-like proteins from bacillus cereus, which we named bcpadr1 and bcpadr2 (pr ...201223189126
[synthesis and antimicrobial ivestigation of 8-quinolinols glucosaminides].in the phase transfer system solid calcium carbonate--acetonitrile per acetylated o-beta-d-glucosaminides of 8-quinolinol, 2-methyl-8-quinolinol and 5-chloro-8-quinolinol were synthesized. zemplen deacetylation was led to corresponding trioles. obtained o-beta-d-glucosaminides were identified with 1h-nmr spectroscopy. antimicrobial activity of deacetylated forms has been studied in a test of inhibition of bioluminescence of marine luminescent bacteria vibrio fischeri f1 and method of the serial ...201323189563
[lead adsorption by trametes gallica, bacillus cereus, and their co-immobilized biomaterial].taking trametes gallica mycelium pellets, bacillus cereus, and their co-immobilized biomaterial as bio-adsorbents, this paper studied their pb2+ adsorption under effects of different contact time, medium initial ph value and pb2+ concentration, and bio-adsorbent concentration, and compared the infrared spectra of the bio-adsorbents before and after pb2+ absorption. the pb2+ adsorption efficiency of the bio-adsorbents was the highest when the bio-adsorbent concentration was 2 g x l(-1), initial p ...201223189701
crystallization and preliminary x-ray data analysis of the pxo1 plasmid-partitioning factor tubz from bacillus cereus.tubz is a structural homologue of tubulin and ftsz gtpases, which are involved in the type iii plasmid-partitioning system. tubz assembles into polymers in a gtp-dependent manner and drives plasmid segregation as `cytomotive' filaments. in this study, c-terminally truncated tubz from bacillus cereus was crystallized in the presence or absence of gdp by the hanging-drop vapour-diffusion method. the crystal of tubz in complex with gdp belonged to the monoclinic space group p2(1), with unit-cell pa ...201223192045
production of medium chain saturated fatty acids with enhanced antimicrobial activity from crude coconut fat by solid state cultivation of yarrowia lipolytica.fatty acids profiles and antimicrobial activity of crude coconut fat hydrolysates obtained in solid-state cultivation system with a selected yeast strain yarrowia lipolytica ro13 were performed. a preliminary step regarding extracellular lipase production and solid state enzymatic hydrolysis of crude fat at different water activity and time intervals up to 7 days was also applied. gas chromatography-mass spectrometry analysis was used for quantification of medium chain saturated fatty acids (mcs ...201323194533
quorum-sensing regulates biofilm formation in vibrio scophthalmi.in a previous study, we demonstrated that vibrio scophthalmi, the most abundant vibrio species among the marine aerobic or facultatively anaerobic bacteria inhabiting the intestinal tract of healthy cultured turbot (scophthalmus maximus), contains at least two quorum-sensing circuits involving two types of signal molecules (a 3-hydroxy-dodecanoyl-homoserine lactone and the universal autoinducer 2 encoded by luxs). the purpose of this study was to investigate the functions regulated by these quor ...201223198796
role of mechanical vs. chemical action in the removal of adherent bacillus spores during cip procedures.this study was designed to evaluate the respective roles of mechanical and chemical effects on the removal of bacillus spores during cleaning-in-place. this analysis was performed on 12 strains belonging to the bacillus cereus group (b. cereus, bacillus anthracis, bacillus thuringiensis) or to less related bacillus species (bacillus pumilus, bacillus licheniformis, bacillus sporothermodurans, bacillus subtilis). adherent spores were subjected to rinsing-in-place (mechanical action) and cleaning- ...201323200646
low temperatures and fermentative metabolism limit peptidoglycan digestion of bacillus cereus. impact on colony forming unit counts.the impact of fermentative metabolism at low temperature on cell division of bacillus cereus was studied. fermentation at 37 °c had no influence on the division of bacteria. aerobic cultures at 15 °c produced larger cells than at 37 °c, but cell division was normal. in fermentative cultures at 15 °c, no increase in cfu ml(-1) was observed. however, a(600) increased, due to formation of long filaments. transmission electronic microscopy and light microscopy with fluorescent staining showed severa ...201323200654
characterisation and profiling of bacillus subtilis, bacillus cereus and bacillus licheniformis by maldi-tof mass fingerprinting.the bacillus genus includes species such as bacillus cereus, bacillus licheniformis and bacillus subtilis, some of which may be pathogenic or causative agents in the spoilage of food products. the main goal of this work was to apply matrix-assisted laser desorption ionisation-time of flight (maldi-tof) mass fingerprinting to the classification of these bacillus species. genetic analyses were also compared to phyloproteomic analyses. a collection of 57 bacillus strains isolated from fresh and pro ...201323200657
bacillus cereus g9241 s-layer assembly contributes to the pathogenesis of anthrax-like disease in mice.bacillus cereus g9241, the causative agent of anthrax-like disease, harbors virulence plasmids encoding anthrax toxins as well as hyaluronic acid (ha) and b. cereus exopolysaccharide (bps) capsules. b. cereus g9241 also harbors s-layer genes, including homologs of bacillus anthracis surface array protein (sap), extractable antigen 1 (ea1), and the s-layer-associated proteins (bsls). in b. anthracis, s-layer proteins and bsls attach via their s-layer homology domains (slh) to the secondary cell w ...201323204457
dna extract characterization process for microbial detection methods development and validation.quantitative polymerase chain reaction (qpcr) assays used in pathogen detection require rigorous methods development including characterizing dna extraction products. a dna extract characterization process is demonstrated using dna extracted from five different cells types (two gram-negatives: escherichia coli, and burkholderia thailandensis, spores and vegetative cells from the gram-positive bacillus cereus, and yeast saccharomyces cerevisiae) with six different methods.201223206278
essential oil of mitracarpus frigidus as a potent source of bioactive compounds.in our previous work (fabri et al. 2009), we showed that different extracts of mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. in order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. thus, the essential oil obtained by hydrodistillation of the aerial parts of m. frigidus was analyzed by gc/ms. among several compounds detected, 11 were identified, bei ...201223207707
variation in the annual unsatisfactory rates of selected pathogens and indicators in ready-to-eat food sampled from the point of sale or service in wales, united kingdom.the welsh food microbiological forum "shopping basket" survey is a long running, structured surveillance program examining ready-to-eat food randomly sampled from the point of sale or service in wales, united kingdom. the annual unsatisfactory rates for selected indicators and pathogens for 1998 through 2008 were examined. all the annual unsatisfactory rates for the selected pathogens were <0.5%, and no pattern with the annual rate was observed. there was also no discernible trend observed for t ...201223212024
comparative proteomic analysis of rice seedlings in response to inoculation with bacillus cereus.reports suggest that bacillus spp. can be used to increase plant growth and resistance to disease, but the molecular mechanisms underlying the interaction between bacillus spp. and plant is not completely understood. in the present study, to clarify these underlying mechanisms, the interaction between bacillus cereus and rice was investigated using two-dimensional gel electrophoresis. through comparative analysis, a total of 31 differentially expressed proteins were obtained upon b. cereus nmsl8 ...201323216197
small bacillus cereus atcc 14579 subpopulations are responsible for cytotoxin k production.bacillus cereus diarrhoeal food poisoning can be caused by several potential enterotoxins, including the nonhaemolytic enterotoxin (nhe), haemolysin bl (hbl) and cytotoxin k (cytk). to get more insights into the cytk expression, a fluorescent reporter strain was created for cytk expression.201323216529
enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of bacillus cereus and ketogulonicigenium vulgare.the co-cultures of ketogulonicigenium vulgare and bacillus cereus were subcultured daily for a total of 150 transfers. the yield of 2-keto-gulonic acid (2-kga) and medium ph in the co-cultures were measured. we found that the serial subcultivation increased the yield of 2-kga from 77% (original co-culture) to 93% (the 150th transfer of transferred co-culture). the resulted strains are of industrial interests for vitamin c production.201323218663
functional annotation analytics of bacillus genomes reveals stress responsive acetate utilization and sulfate uptake in the biotechnologically relevant bacillus megaterium.bacillus species form an heterogeneous group of gram-positive bacteria that include members that are disease-causing, biotechnologically-relevant, and can serve as biological research tools. a common feature of bacillus species is their ability to survive in harsh environmental conditions by formation of resistant endospores. genes encoding the universal stress protein (usp) domain confer cellular and organismal survival during unfavorable conditions such as nutrient depletion. as of february 20 ...201223226010
decolorization of azo dye reactive black b by bacillus cereus strain hj-1.reactive black b (rbb) is a group of azo dyes that are widely used in the textile industry. in this study, a new microbial strain was isolated from azo dye contaminated river sediment which is capable of degrading rbb. the strain was identified as bacillus cereus strain hj-1 by 16s rrna gene sequences analysis. the optimal conditions for rbb decolorization by b. cereus strain hj-1 are: 25°c, ph 8, 1 cmc of triton x-100, 0.15 g l(-1) of added yeast extract, 0.125 g l(-1) of added glucose and stat ...201323228911
antibacterial, antioxidant and cytotoxic studies of total saponin, alkaloid and sterols contents of decoction of joshanda: identification of components through thin layer chromatography.the current study was aimed to assess antibacterial, antioxidant and cytotoxic activity of total saponin, alkaloid and sterol contents of joshanda decoction followed by its constituent's analysis via thin layer chromatography (tlc). saponins and alkaloids showed prominent antibacterial activity against staphylococcus aureus, bacillus subtilis, bacillus cereus and klebsiella pneumoniae whereas sterols only against s. aureus. saponin and alkaloid contents of 97 and 108 µg/ml, respectively, showed ...201523235996
enterotoxin production by bacillus cereus under gastrointestinal conditions and their immunological detection by commercially available kits.currently, three commercial kits for bacillus cereus enterotoxins nhe and/or hbl detection are available, namely, the bacillus diarrheal enterotoxin visual immunoassay (bde via™) kit (3m tecra), b. cereus enterotoxin reversed passive latex agglutination (bcet-rpla) kit (oxoid), and the duopath(®) cereus enterotoxins (merck). the performance of the kits and their applicability to gastrointestinal simulation samples were evaluated. then, the stability and production of enterotoxins hbl and nhe und ...201223237409
plcra, a new quorum-sensing regulator from bacillus cereus, plays a role in oxidative stress responses and cysteine metabolism in stationary phase.we characterized a new quorum-sensing regulator, plcra, which is present in various members of the b. cereus group and identified a signaling heptapeptide for plcra activity: papra(7). we demonstrated that plcra is a 3d structural paralog of plcr using sequence analysis and homology modeling. a comparison of the transcriptomes at the onset of stationary phase of a δplcra mutant and the wild-type b. cereus atcc 14579 strain showed that 68 genes were upregulated and 49 genes were downregulated in ...201223239999
growth/no growth models for heat-treated psychrotrophic bacillus cereus spores under cold storage.the microbiological safety of refrigerated and processed foods of extended durability (repfed) is linked to spore-forming pathogens, more specifically clostridium botulinum and bacillus cereus. in this study two sets of growth/no growth (gng) models are presented for the spores of two b. cereus strains. the models incorporate both product (water activity (a(w)) and ph) and process parameters (pasteurization value at 90 °c (p(90)) or heating temperature). the first model evaluates the effect of f ...201323246607
screening for bacillus subtilis group isolates that degrade cyanogens at ph 4.5-5.0.cyanogenic food crops abound in nature with important crops like cassava forming the staple food for over half a billion people. detoxification by hydrolysis of cassava cyanogenic glycosides often involves acid fermentation, and in some of these processes bacillus species are encountered. forty bacillus spp. (20 bacillus subtilis, 11 bacillus licheniformis, 7 bacillus sonorensis, 2 bacillus cereus) isolated from acid fermented primary starters to produce gergoush, a sudanese fermented snack, wer ...201323261810
synthesis, pharmacological activity evaluation and molecular modeling of new polynuclear heterocyclic compounds containing benzimidazole derivatives.novel heterocyclic compounds containing benzimidazole derivatives were synthesized from 2-(1h-benzimidazol-2-yl) acetonitrile (1) and arylhydrazononitrile derivative 2 was obtained via coupling of 1 with 4-methyl phenyldiazonium salt, which was then reacted with hydroxylamine hydrochloride to give amidooxime derivative 3. this product was cyclized into the corresponding oxadiazole derivative 4 upon reflux in acetic anhydride. compound 4 was refluxed in dmf in the presence of triethylamine to giv ...201223263801
supramolecular coordination and antimicrobial activities of constructed mixed ligand complexes.a novel series of copper(ii) and palladium(ii) with 4-derivatives benzaldehyde pyrazolone (l(n)) were synthesized. the mixed ligand complexes were prepared by using 1,10-phenanthroline (phen) as second ligand. the structure of these complexes was identified and confirm by elemental analysis, molar conductivity, uv-vis, ir and (1)h nmr spectroscopy and magnetic moment measurements as well as thermal analysis. the ligand behaves as a neutral bidentate ligand through on donor sites. esr spectra sho ...201223268204
lc-ms analysis of the emetic toxin, cereulide, produced by bacillus cereus.a quantitative and chemical assay of cereulide produced in the cultures by some strains of bacillus cereus was performed on a hplc and a esi electrospray ion trap mass analyzer, using the synthetic cereulide as a standard. all 20 strains of emetic b. cereus were found to produce 27 - 740 ng/ml of cereulide by the lc-ms analysis. in contrast, none of the 10 diarrheal strains produced it. 10(2) cfu/ml of the cereulide producible strain with a 210 ng/ml yield was inoculated into the 10% suspensions ...201223269221
the insect galleria mellonella as a powerful infection model to investigate bacterial pathogenesis.the study of bacterial virulence often requires a suitable animal model. mammalian models of infection are costly and may raise ethical issues. the use of insects as infection models provides a valuable alternative. compared to other non-vertebrate model hosts such as nematodes, insects have a relatively advanced system of antimicrobial defenses and are thus more likely to produce information relevant to the mammalian infection process. like mammals, insects possess a complex innate immune syste ...201223271509
changes in microbial contamination levels of porcine carcasses and fresh pork in slaughterhouses, processing lines, retail outlets, and local markets by commercial distribution.the objective of this study was to evaluate the changes in microbial contamination levels of each porcine carcass and fresh pork in a general distribution process. a total of 100 commercial pigs were sampled (six sampling sites per individual, total 600 samples) at four sequential stages: slaughterhouse (after carcass grading and boning), processing line, retail outlet, and local market. no significant differences were observed in the contaminant percentages among sampling sites and sample colle ...201323273786
structure of gentlyase, the neutral metalloprotease of paenibacillus polymyxa.gentlyase is a bacterial extracellular metalloprotease that is widely applied in cell culture and for tissue dissociation and that belongs to the family of thermolysin-like proteases. the structure of thermolysin has been known since 1972 and that of bacillus cereus neutral protease since 1992. however, the structure determination of other bacillus neutral proteases has been hindered by their tendency to cannibalistic autolysis. high calcium conditions that allow the concentration and crystalliz ...201223275160
structural basis for the activation mechanism of the plcr virulence regulator by the quorum-sensing signal peptide papr.the quorum-sensing regulator plcr is the master regulator of most known virulence factors in bacillus cereus. it is a helix-turn-helix (hth)-type transcription factor activated upon binding of its cognate signaling peptide papr on a tetratricopeptide repeat-type regulatory domain. the structural and functional properties of plcr have defined a new family of sensor regulators, called the rnpp family (for rap, nprr, prgx, and plcr), in gram-positive bacteria. to fully understand the activation mec ...201323277548
sporulation environment of emetic toxin-producing bacillus cereus strains determines spore size, heat resistance and germination capacity.heat resistance, germination and outgrowth capacity of bacillus cereus spores in processed foods are major factors in causing the emetic type of gastrointestinal disease. in this study, we aim to identify the impact of different sporulation conditions on spore properties of emetic toxin-producing b. cereus strains.201323279596
induction of drought tolerance in cucumber plants by a consortium of three plant growth-promoting rhizobacterium strains.our previous work showed that a consortium of three plant growth-promoting rhizobacterium (pgpr) strains (bacillus cereus ar156, bacillus subtilis sm21, and serratia sp. xy21), termed as bbs for short, was a promising biocontrol agent. the present study investigated its effect on drought tolerance in cucumber plants. after withholding watering for 13 days, bbs-treated cucumber plants had much darker green leaves and substantially lighter wilt symptoms than control plants. compared to the control ...201223285089
heterologous expression of cyp102a5 variant from bacillus cereus cyppb-1: validation of model for predicting drug metabolism of human p450 probe substrates.cyp102a5 variant (adl27534) from isolated bacillus cereus cyppb-1 was heterologously expressed in escherichia coli top 10 cells. comparative sequence analysis of purified cyp102a5 variant with respect to reported cyp102a5 (aap10153) from bacillus cereus atcc 14579 revealed amino acid sequence changes at positions p245s and m318i of heme domain. the binding affinities of 15 selected human p450 probe substrates towards isolated cyp102a5 were analyzed in silico using a homology model together with ...201323287855
assessment of the microbial safety and quality of cooked chilled foods and their production process.refrigerated processed foods of extended durability (repfeds) are a heterogeneous group of food products. this study assesses the microbial safety and quality along the production process in five repfed companies. samples were taken of raw materials (n=123), intermediate products (n=123), end products at production day (n=45) and at end of shelf life (n=90), food contact surfaces (n=226) and worker's hands/gloves (n=92). samples are analysed for total psychrotrophic aerobic count, aerobic spore ...201323290224
inactivation of foodborne pathogens in ground beef by cooking with highly controlled radio frequency energy.the consumer demand for fresh tasting, high quality, low salt, preservative-free meals which require minimal preparation time magnifies the safety concern and emphasizes the need to use innovative technologies for food processing. a modern technique to uniformly heat and cook foods is based on a combination of convection and controlled radio frequency (rf) energy. however any advantage conferred on meat cooked by this method would be lost if application of the technology results in decreased saf ...201323290228
combined effects of plant extracts in inhibiting the growth of bacillus cereus in reconstituted infant rice cereal.a study was done to determine the potential use of plant extracts to inhibit the growth of bacillus cereus in reconstituted infant rice cereal. a total of 2116 extracts were screened for inhibitory activity against b. cereus using an agar well diffusion assay. the minimal inhibitory concentrations (mic) and minimal lethal concentrations (mlc) of 14 promising extracts in tryptic soy broth (tsb) were determined. dryopteris erythrosora (autumn fern) root extract showed the lowest mic (0.0156 mg/ml) ...201323290233
combined effects of ultrasound and surfactants to reduce bacillus cereus spores on lettuce and carrots.this study was performed to compare the effectiveness of ultrasound treatment singly and in combination with surfactants as an alternative method to conventional sanitizers containing chlorine for reducing numbers of bacillus cereus spores on fresh produce. a cocktail of three strains of b. cereus (10876, atcc 13061, and w-1) spores was inoculated onto iceberg lettuce and then treated with ultrasound for 0, 5, 10, 20 and 60 min. five minutes was found to be an adequate ultrasound (40 khz, 30 w/l ...201323290247
bacillus cereus necrotizing pneumonia in a patient with nephrotic syndrome.bacillus cereus (b. cereus) is a gram-positive rod that is widely distributed in the environment and can be a cause of food poisoning. we herein present a case of b. cereus necrotizing pneumonia in a patient with nephrotic syndrome under corticosteroid treatment after developing transient gastroenteritis symptoms. b. cereus was isolated from bronchial lavage fluid and transbronchial biopsy specimens. a multiplex polymerase chain reaction analysis of the toxin genes revealed a strain possessing e ...201323291682
study of charge distribution on the surface of biocolloids.potentiometric titration and zeta potential measurements are crucial techniques for the characterization of the surface properties of bacterial cells. in this study, we investigated the effects of two commonly used electrolytes, nano(3) and naclo(4), on the viability and acid-base properties of gram-positive bacteria bacillus subtilis. b. subtilis are non-pathogenic bacteria which are often used to model the surface properties of pathogenic microorganisms of the same genus, including bacillus an ...201323298596
purification of an antifungal compound, cyclo(l-pro-d-leu) for cereals produced by bacillus cereus subsp. thuringiensis associated with entomopathogenic nematode.mold spoilage is the main cause of substantial economic loss in cereals and might also cause public health problems due to the production of mycotoxins. the aim of this study was to separate and purify and to identify antifungal compounds of bacterium associated with novel entomopathogenic nematode and check the antifungal property of identified compound in particular food model systems. the antifungal compound was purified using silica gel column chromatography, tlc and hplc and its structure w ...201323305769
bacillus cereus sphingomyelinase recognizes ganglioside gm3.sphingomyelinase (smase) from bacillus cereus (bc-smase) hydrolyzes sphingomyelin (sm) to phosphocholine and ceramide in a divalent metal ion-dependent manner, and is a virulence factor for septicemia. bc-smase has three characteristic sites, viz., the central site (catalytic site), side-edge site (membrane binding site), and β-hairpin region (membrane binding site). here, we show that the β-hairpin directly binds to gangliosides, especially neuacα2-3galβ1-4glcβ1-1ceramide (gm3) through a carboh ...201323313504
inhibitory effect of cinnamon powder on pathogen growth in laboratory media and oriental-style rice cakes (sulgidduk).there has been an increasing interest in the use of natural plant materials as alternative food preservatives. we examined the antimicrobial effects of natural plant materials used as additives against foodborne pathogens in laboratory media and sulgidduk, oriental-style rice cakes. cinnamon, mugwort, and garlic powder solutions (3%) were tested for their antimicrobial activities against pathogens in laboratory media. sulgidduk prepared with different amounts of cinnamon powder (1, 3, and 6%) wa ...201323317869
vaccine protection against bacillus cereus-mediated respiratory anthrax-like disease in mice.bacillus cereus strains harboring a pxo1-like virulence plasmid cause respiratory anthrax-like disease in humans, particularly in welders. we developed mouse models for intraperitoneal as well as aerosol challenge with spores of b. cereus g9241, harboring pbcxo1 and pbc218 virulence plasmids. compared to wild-type b. cereus g9241, spores with a deletion of the pbcxo1-carried protective antigen gene (paga1) were severely attenuated, whereas spores with a deletion of the pbc218-carried protective ...201323319564
calcium-ion-induced stabilization of the protease from bacillus cereus wq9-2 in aqueous hydrophilic solvents: effect of calcium ion binding on the hydration shell and intramolecular interactions.the neutral protease wq from bacillus cereus is stable in various aqueous organic mixtures, with the exception of those containing acetonitrile (acn) and dimethylformamide (dmf). the stability of the enzyme in aqueous hydrophilic solvents was dramatically enhanced with the addition of calcium ions, with the degree of improvement in the half-life relative to different solutions ranging from fourfold to more than 70-fold. studies of the kinetic constants showed that calcium ions induced slight con ...201323322168
design, synthesis, antimicrobial activity and anti-hiv activity evaluation of novel hybrid quinazoline-triazine derivatives.a series of novel hybrid quinazoline-triazine derivatives was designed and synthesized from cyanuric chloride and anthranilic acid through sequential reactions, which contain different pharmacophores like quinazoline and substituted diaryl triazine (data) linked with ethylene diamine. all the newly synthesized compounds were characterized by infrared, (1)h-nmr, (13)c-nmr, ms and elemental analysis. further, we evaluated the in vitro anti-hiv activity of the newly synthesized compounds against hi ...201423327639
screening antimicrobial activity of various extracts of urtica dioica.urtica dioica or stinging nettle is traditionally used as an herbal medicine in western asia. the current study represents the investigation of antimicrobial activity of u. dioica from nine crude extracts that were prepared using different organic solvents, obtained from two extraction methods: the soxhlet extractor (method i), which included the use of four solvents with ethyl acetate and hexane, or the sequential partitions (method ii) with a five solvent system (butanol). the antibacterial an ...201223342511
transport and retention of selected engineered nanoparticles by porous media in the presence of a biofilm.column experiments were conducted to investigate the transport of aqueous c60 (aqu-nc60), fullerol, silver nanoparticles (nps) coated with polyvinylpyrrolidone (ag-pvp) and stabilized by citrate (ag-cit) in biofilm-laden porous media. gram-negative pseudomonas aeruginosa (pa) and gram-positive bacillus cereus (bc) biofilm-laden glass beads were selected to represent the bacterial interfaces nps might encounter in the natural aquatic environment. the biomass distribution, extracellular polymeric ...201323346937
biofilm inhibitors that target amyloid proteins.bacteria establish stable communities, known as biofilms, that are resistant to antimicrobials. biofilm robustness is due to the presence of an extracellular matrix, which for several species-among them bacillus subtilis-includes amyloid-like protein fibers. in this work, we show that b. subtilis biofilms can be a simple and reliable tool for screening of molecules with antiamyloid activity. we identified two molecules, aa-861 and parthenolide, which efficiently inhibited biofilms by preventing ...201323352144
antimicrobial and antifungal activities of lactobacillus curvatus strain isolated from homemade azerbaijani cheese.the aims of this study were to characterize inhibitory activity spectra, some probiotic properties and safety of lactobacillus curvatus a61 for its future application in production of fermented foods. the studied strain was isolated from traditional homemade cheese manufactured in azerbaijan. the cell-free supernatant of culture of lb. curvatus a61 inhibited the growth of tested lab, as well as of listeria monocytogenes and bacillus cereus strains. the strain presented antifungal activity and in ...201323357316
decolorization and detoxification of two textile industry effluents by the laccase/1-hydroxybenzotriazole system.the aim of this work was to determine the optimal conditions for the decolorization and the detoxification of two effluents from a textile industry-effluent a (the reactive dye bath bezactive) and effluent b (the direct dye bath tubantin)-using a laccase mediator system. response surface methodology (rsm) was applied to optimize textile effluents decolorization. a box-behnken design using rsm with the four variables ph, effluent concentration, 1-hydroxybenzotriazole (hbt) concentration, and enzy ...201323361176
[analysis of bactericidal material generated by electrical devices advertising bactericidal ability against bacteria on the agar gel plates].several japanese companies sell electrical devices advertised as effective in inactivating viruses and killing bacteria by releasing special materials, e.g., plasmacluster ions, nanoe particle and minus ions, into the air. these companies claim that their devices killed bacteria on plates in their own experiments. we tested device effectiveness using the same experiments from the plasmacluster ioniser sharp co., japan, the nanoe generator panasonic co., japan, and the vion king jim co., japan, t ...201223367847
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