Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| identification of listeria monocytogenes in vivo-induced genes by fluorescence-activated cell sorting. | listeria monocytogenes is a gram-positive, intracellular, food-borne pathogen capable of causing severe infections in immunocompromised or pregnant individuals, as well as numerous animal species. genetic analysis of listeria pathogenesis has identified several genes which are crucial for virulence. the transcription of most of these genes has been shown to be induced upon entry of listeria into the host cell. to identify additional genes that are induced in vivo and may be required for l. monoc ... | 2001 | 11447181 |
| systemic and intracerebral infections of mice with listeria monocytogenes successfully treated with linezolid. | linezolid is an oxazolidinone derivative which is active mostly against gram-positive bacteria. in this work its activity against the facultatively intracellular bacterium listeria monocytogenes was examined in vitro, in tissue culture and in animal models of systemic and intracerebral infection and compared with ampicillin which is the antibiotic of choice for treatment of listeriosis. all strains of l. monocytogenes were susceptible to the substance, with minimal inhibitory concentrations (mic ... | 2001 | 11450884 |
| activities of gemifloxacin and five other antimicrobial agents against listeria monocytogenes and coryneform bacteria isolated from clinical samples. | the in vitro activities of gemifloxacin, ciprofloxacin, ampicillin, doxycycline, gentamicin, and vancomycin were evaluated against 15 listeria monocytogenes strains and 205 coryneform bacteria isolated from clinical samples. the percentages of strains inhibited by gemifloxacin at 0.5 microg/ml were 100% (l. monocytogenes), 93.3% (brevibacterium spp.), 90% (corynebacterium minutissimum), 42.5% (corynebacterium amycolatum), 20% (corynebacterium striatum), 12.5% (corynebacterium jeikeium), and 10% ... | 2001 | 11451706 |
| effect of age on respiratory defense mechanisms: pulmonary bacterial clearance in fischer 344 rats after intratracheal instillation of listeria monocytogenes. | to examine the lung defense mechanisms of both young and aged rats before and after pulmonary challenge with a bacterial pathogen. | 2001 | 11451845 |
| optimizing detection of heat-injured listeria monocytogenes in pasteurized milk. | optimal conditions for the detection of heat-injured cells of listeria monocytogenes in modified pennsylvania state university (mpsu) broth were determined using a response surface design generated by a computer program, echip. different combinations of incubation temperatures and lithium, magnesium, and d-serine concentrations were evaluated to determine the optimum conditions for the detection of heat-injured l. monocytogenes in filter-sterilized whole milk inoculated with selected problematic ... | 2001 | 11456184 |
| antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. | the beneficial health effects of extracts from many types of plants that are used as seasoning agents in foods and beverages have been claimed for centuries. the purpose of this study was to examine the effectiveness of selected herb and spice essential oils for control of growth and survival of microorganisms. inhibition of growth was tested by the paper disc agar diffusion method. antibiotic susceptibility discs were used as control. minimum lethal concentration (mlc) was determined by the tub ... | 2001 | 11456186 |
| evaluation of thin agar layer method for recovery of acid-injured foodborne pathogens. | the thin agar layer (tal) method of kang and fung was used to enumerate acid-injured foodborne pathogens. this method involves overlaying 14 ml of nonselective medium (tryptic soy agar [tsa]) onto a prepoured and solidified pathogen-specific, selective medium in a petri dish. after surface plating, injured cells resuscitated and grew on tsa during the first few hours of incubation; then, the selective agents from the selective medium diffused to the top layer, interacted with the recovered micro ... | 2001 | 11456194 |
| fate of escherichia coli o157:h7, salmonella typhimurium dt 104, and listeria monocytogenes in fresh meat decontamination fluids at 4 and 10 degrees c. | bacterial pathogens may colonize meat plants and increase food safety risks following survival, stress hardening, or proliferation in meat decontamination fluids (washings). the objective of this study was to evaluate the ability of escherichia coli o157:h7, salmonella typhimurium dt 104, and listeria monocytogenes to survive or grow in spray-washing fluids from fresh beef top rounds sprayed with water (10 or 85 degrees c) or acid solutions (2% lactic or acetic acid, 55 degrees c) during storage ... | 2001 | 11456202 |
| survival of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in and on vacuum packaged lebanon bologna stored at 3.6 and 13.0 degrees c. | escherichia coli o157:h7, salmonella typhimurium, or listeria monocytogenes was spread onto the surface of lebanon bologna luncheon slices using sterile glass rods. the inoculated slices were stacked and vacuum packaged. the packages were stored at 3.6 or 13 degrees c. the foodborne pathogens. e. coli o157:h7, salmonella typhimurium, or l. monocytogenes were reduced in lebanon bologna during storage at 3.6 or 13 degrees c. the higher storage temperature (13.0 degrees c) resulted in significantly ... | 2001 | 11456203 |
| prevalence of listeria monocytogenes in broilers at the abattoir, processing plant, and retail level. | the environment and products from two broiler abattoirs and processing plants and raw broiler pieces at the retail level were sampled for listeria monocytogenes in order to evaluate the contamination level of the broiler carcasses and products. sampling started in the slaughtering process and finished with raw broiler meat or ready-to-eat cooked product. sampling sites positive for l. monocytogenes at the broiler abattoir were the air chiller, the skin-removing machine, and the conveyor belt lea ... | 2001 | 11456209 |
| outbreak of listeriosis associated with homemade mexican-style cheese--north carolina, october 2000-january 2001. | on november 13, 2000, health-care providers at a hospital in winston-salem, north carolina, contacted the local health department about three cases of listeriosis within a 2-week period in recent mexican immigrants. the north carolina general communicable disease control branch, in collaboration with the forsyth county health department, the north carolina departments of agriculture and consumer services (ncda&cs) and environment and natural resources, the food and drug administration (fda), and ... | 2001 | 11456331 |
| protective ctl response is induced in the absence of cd4+ t cells and ifn-gamma by gene gun dna vaccination with a minigene encoding a ctl epitope of listeria monocytogenes. | our work was undertaken to learn the mechanism of induction of protective cytotoxic t lymphocytes (ctl) by gene gun dna vaccination with p91m encoding an h-2kd-restricted t cell epitope of listeriolysin o (llo). vaccination with p91m induced vigorous antigen-specific cd8+ ctl that produce ifn-gamma and was able to confer partial protection against listerial challenge. however, the p91m-induced protective immunity was revealed to be independent of the ifn-gamma and cd4+ t cell help. the ctl induc ... | 2001 | 11457557 |
| inactivation of lrg-47 and irg-47 reveals a family of interferon gamma-inducible genes with essential, pathogen-specific roles in resistance to infection. | the cytokine interferon (ifn)-gamma regulates immune clearance of parasitic, bacterial, and viral infections; however, the underlying mechanisms are poorly understood. recently, a family of ifn-gamma-induced genes has been identified that encode 48-kd gtp-binding proteins that localize to the endoplasmic reticulum of cells. the prototype of this family, igtp, has been shown to be required for host defense against acute infections with the protozoan parasite toxoplasma gondii, but not for normal ... | 2001 | 11457893 |
| retrospective evidence for nosocomial listeria infection. | 2001 | 11461136 | |
| recovery of intraoperative microbicidal and inflammatory functions of alveolar immune cells after a tobacco smoke-free period. | tobacco smoking inhibits alveolar macrophage function, but cessation of smoking markedly reduces the risk of postoperative pulmonary complications. the authors therefore evaluated the effect of nonsmoking duration on both antimicrobial and inflammatory functions of alveolar macrophages during anesthesia and surgery. | 2001 | 11465626 |
| brain abscess due to listeria monocytogenes: five cases and a review of the literature. | 2001 | 11470983 | |
| initiation of alcoholic fatty liver and hepatic inflammation with a specific recall immune response in alcohol-consuming c57bl/6 mice. | whether immunological responses are involved in initiation and progression of alcoholic liver disease is unclear. we describe a mouse model of alcoholic liver injury characterized by steatosis and hepatic inflammation initiated by a recall immune response. mice immune to listeria monocytogenes fed a liquid diet containing ethanol and challenged with viable bacteria developed steatosis within 24 h and, at a later time, elevated serum alanine aminotransferase levels, indicating more liver damage i ... | 2001 | 11472435 |
| automated ribotyping using different enzymes to improve discrimination of listeria monocytogenes isolates, with a particular focus on serotype 4b strains. | to develop improved automated subtyping approaches for listeria monocytogenes, we characterized the discriminatory power of different restriction enzymes for ribotyping. when 15 different restriction enzymes were used for automated ribotyping of 16 selected l. monocytogenes isolates, the restriction enzymes ecori, pvuii, and xhoi showed high discriminatory ability (simpson's index of discrimination > 0.900) and produced complete and reproducible restriction cut patterns. these three enzymes were ... | 2001 | 11474034 |
| [microbial growth and cold. study of the particular case of listeria monocytogenes]. | some of micro-organisms are able to adapt themselves to hardest environmental conditions. so, about cold conditions, most of them can still grow at very low temperatures under 0 degree celsius (nitrogen liquefied at -169 degrees c is the best way for preserving microbes). by another way some germs, qualified psychrotophic bacteria, grow quite easily between 0 degree c and +10 degrees c. among these bacteria the author draw the attention to listeria monocytogenes, a germ contaminating often foods ... | 2001 | 11474584 |
| detection of anti-listeriolysin o and listeria monocytogenes in experimentally infected buffaloes (bubalus bubalis). | the kinetics of antibody production against listeriolysin o (allo) and the recovery pattern of listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic l. monocytogenes. antibodies to llo appeared by 7-10 days post infection (pi), with a shallow peak between days 16 and 36 pi, when tested by indirect plate-elisa. the titres of allo in all the animals then declined slowly but remained detectable up to day 7 ... | 2001 | 11474862 |
| listeria monocytogenes: a causative agent of gastroenteritis? | the intestinal tract is the major portal of entry for listeria monocytogenes, which becomes ingested via contaminated food. the pathogenic strains penetrate the mucosal tissue either directly, via engulfment by enterocytes, or indirectly, via active penetration of the peyer's patches. there are now several reports clearly demonstrating that, in some cases, acute enteritis may be the only symptom of listeria infection or may precede the typical symptoms of listeriosis such as sepsis and meningiti ... | 2001 | 11476434 |
| dicynthaurin: an antimicrobial peptide from hemocytes of the solitary tunicate, halocynthia aurantium. | we isolated a novel antimicrobial peptide, dicynthaurin, from hemocytes of a tunicate, halocynthia aurantium. the native peptide had a mass of approximately 6.2 kda and was composed of two 30-residue monomers without sequence homology to any previously identified peptides (ilqkavldclkaagsslskaaitaiynkit). most cynthaurin molecules were c-terminally amidated and were linked covalently by a single cystine disulfide bond. when performed in membrane-mimetic environments, circular dichroism studies o ... | 2001 | 11479030 |
| effects of trimethoprim and co-trimoxazole on the morphology of listeria monocytogenes in culture medium and after phagocytosis. | the purpose of this study was to compare the extra- and intracellular activity of antifolates on listeria monocytogenes. the fortuitous discovery of elongated bacteria in response to trimethoprim revealed a novel effect on the morphology of listeria in cell culture medium and after phagocytosis. this phenomenon permitted the quantification of trimethoprim activity, revealing comparable activity intra- and extracellularly. subinhibitory concentrations of trimethoprim resulted in bacterial elongat ... | 2001 | 11481287 |
| the in vitro activity of daptomycin against 514 gram-positive aerobic clinical isolates. | the in vitro activity of daptomycin was assessed in comparison with that of vancomycin and penicillin against a wide range of gram-positive aerobic clinical isolates. mics were determined by an agar dilution method on mueller-hinton agar (nccls/eucast) and on isotonic agar adjusted to contain 50 mg/l free calcium (bsac). both media were enriched with 5% horse blood for fastidious organisms. daptomycin mics for all 172 staphylococci, including methicillin-susceptible and methicillin-resistant sta ... | 2001 | 11481291 |
| implication of milk and milk products in food-borne diseases in france and in different industrialised countries. | a study was carried out to estimate the proportion of diseases due to milk and milk products among food-borne diseases recorded in france and in other countries since 1980. particular attention was given to whether the milk involved was heat-treated or not. four etiologic agents were considered: salmonella spp., staphylococcus aureus, listeria monocytogenes, and pathogenic escherichia coli. an overview of food-borne disease annual reports from seven countries indicated that milk and milk product ... | 2001 | 11482557 |
| measurements and predictions of growth for listeria monocytogenes and salmonella during fluctuating temperature ii. rapidly changing temperatures. | growth of listeria monocytogenes and salmonella was examined during various rates of increase and decrease in temperature from and to the minimum for growth. growth was little affected by even the most rapid changes and injury or lag was not observed. subsequent investigations of growth during periods of rapid variation in temperature from and to temperatures below the growth minimum again had little effect and growth was satisfactorily predicted using the dynamic model of baranyi and roberts [i ... | 2001 | 11482561 |
| acta and human zyxin harbour arp2/3-independent actin-polymerization activity. | the actin cytoskeleton is a dynamic network that is composed of a variety of f-actin structures. to understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. we found that human zyxin and the related protein acta of listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (vasp) manner, but independently of the arp2/3 ... | 2001 | 11483954 |
| m-csf transgenic mice: role of m-csf in infection and autoimmunity. | in this study, transgenic cd2f1 mouse lines (c-1.1-c-1.11) bearing a transgene encoding the murine growth factor m-csf under the control of the liver specific alpha-1-antitrypsin gene promoter were generated. transgenic c-1.4 mice showed elevated expression of transgene-encoded m-csf in the liver and displayed a 2-3-fold increase of m-csf plasma levels and of macrophage numbers in the liver as compared with non-transgenic littermates. m-csf transgenic mice showed increased resistance against sub ... | 2001 | 11484835 |
| acta from listeria monocytogenes can interact with up to four ena/vasp homology 1 domains simultaneously. | the facultative intracellular human pathogenic bacterium listeria monocytogenes actively recruits host actin to its surface to achieve motility within infected cells. the bacterial surface protein acta is solely responsible for this process by mimicking fundamental steps of host cell actin dynamics. acta, a modular protein, contains an n-terminal actin nucleation site and a central proline-rich motif of the 4-fold repeated consensus sequence fpppp (fp(4)). this motif is specifically recognized b ... | 2001 | 11489888 |
| a critical role for interleukin 18 in primary and memory effector responses to listeria monocytogenes that extends beyond its effects on interferon gamma production. | the stimulation of interferon (ifn)-gamma by interleukin (il)-12 has been shown to provide protection from intracellular pathogens such as listeria monocytogenes. tumor necrosis factor (tnf) is also a major player in the resolution of listeria infections and is suggested to have more global effects than can be explained by the induction of ifn-gamma alone. since il-18 synergizes with il-12 to induce ifn-gamma production by natural killer and t helper (th)1 cells, we determined its role in respon ... | 2001 | 11489953 |
| dynamic antigen presentation patterns of listeria monocytogenes-derived cd8 t cell epitopes in vivo. | little information exists regarding the presentation of antigenic peptides in infected tissues. in this study the in vivo presentation of four different cd8 t cell epitopes of listeria monocytogenes was monitored. peptide presentation was measured by a new, highly sensitive, ex vivo ag presentation assay that was based on the testing of freshly isolated cells from infected spleens with peptide-specific cd8 t cell lines in an ifn-gamma-specific elispot assay. remarkably, the peptide presentation ... | 2001 | 11490007 |
| starvation survival in listeria monocytogenes: characterization of the response and the role of known and novel components. | the starvation survival response (ssr) of listeria monocytogenes egd is induced under glucose- or multiple-nutrient-, but not amino-acid limitation. 0.01-0.2% of the population remain viable even after 20 d and the survivors show a reduced cell size and increased cross-protection to several environmental stresses. the development of the ssr may therefore be important in l. monocytogenes survival in the food environment. the initiation, but not the maintenance, of the ssr involves both protein an ... | 2001 | 11496004 |
| curved tails in polymerization-based bacterial motility. | the curved actin "comet-tail" of the bacterium listeria monocytogenes is a visually striking signature of actin polymerization-based motility. similar actin tails are associated with shigella flexneri, spotted-fever rickettsiae, the vaccinia virus, and vesicles and microspheres in related in vitro systems. we show that the torque required to produce the curvature in the tail can arise from randomly placed actin filaments pushing the bacterium or particle. we find that the curvature magnitude det ... | 2001 | 11497617 |
| induction of specific cytotoxic lymphocytes in mice vaccinated with brucella abortus rb51. | a safe, more sensitive, nonradioactive, neutral red uptake assay was adopted to replace the traditional 51cr release assay for detection of brucella-specific cytotoxic t lymphocyte (ctl) activity. our studies indicated that brucella abortus strain rb51 vaccination of mice induced specific ctls against both strain rb51- and strain 2308-infected j774.a1 macrophages but not against listeria monocytogenes-infected j774.a1 cells. the antigen-specific cytotoxic activity was exerted by t lymphocytes bu ... | 2001 | 11500423 |
| development of a competitive index assay to evaluate the virulence of listeria monocytogenes acta mutants during primary and secondary infection of mice. | we developed a competitive index assay for murine listeriosis that tests the virulence of listeria monocytogenes strains in different organs and at various times postinoculation. studies presented here demonstrate the reproducibility of this assay during primary and secondary infection of inbred and outbred mice. we verified the validity of this assay by performing competitive index analysis of a well-characterized strain of l. monocytogenes lacking the acta gene. in addition, we found that whil ... | 2001 | 11500481 |
| effect of enterocin ccm 4231 on listeria monocytogenes in saint-paulin cheese. | the bacteriocin production by enterococcus faecium strain in cheese milk and cheese was demonstrated. purified enterocin ccm 4231 exhibited an anti-listerial effect during saint-paulin cheese manufacture. during cheese production the strain grew to a final concentration of 10.1 +/- 0.01 log cfu per ml per g in cheese. then only a slight decrease of the cell concentration was noticed during ripening and was almost stable for 8 weeks. no significant differences in ph were observed between the expe ... | 2001 | 11501405 |
| production of bacteriocins by different enterococcal isolates. | bacteriocins produced by ruminal as well as environmental isolates were found to be heat-stable antimicrobial substances with a broad inhibitory spectrum (including gram-negative species and sanitary-important species). the exponential phase of growth and ph 4-7 were optimal for their production. after purification, some of them were sequenced and specified. | 2001 | 11501476 |
| recombinant listeria strains producing the nontoxic l-chain of botulinum neurotoxin a in a soluble form. | fragments of clostridium botulinum neurotoxin a (bont/a) gene (bota) were expressed in listeria monocytogenes atcc10527 to produce the l-chain of the toxin in a soluble form. a shuttle vector pat19 (emr) was used to make plasmid pat-rl containing a bota gene fragment placed under c. botulinum ntnh-gene promoter control. the plasmid also contained a c. botulinum botr/a gene, a positive transcriptional regulator of bota. the cytoplasmic fraction of the l. monocytogenes (pat-rl) strain was found to ... | 2001 | 11501674 |
| sensitivity and specificity of the bax for screening/listeria monocytogenes assay: internal validation and independent laboratory study. | in this study to certify the bax for screening/listeria monocytogenes assay (dupont qualicon, wilmington, de), an internal evaluation was conducted on 16 food types that were simultaneously analyzed with the bax system (bax), and the iso method for the detection of l. monocytogenes (iso). no statistically significant difference in performance between the bax and iso methods was observed. inclusivity/exclusivity testing showed that the bax system was able to detect 97 of 97 (100%) of l. monocytog ... | 2001 | 11501909 |
| efficacies of abt-773, a new ketolide, against experimental bacterial infections. | abt-773 is a novel ketolide effective against antibacterial-resistant respiratory tract pathogens. the pharmacokinetic profile of abt-773 was studied in rats and consisted of a mean peak concentration in plasma of 1.07 microg/ml and an area under the concentration-time curve (auc) of 12.03 microg. h/ml when the compound was delivered at a dose of 25 mg/kg of body weight. it concentrated in rat lung tissue, with a lung tissue-to-plasma ratio of 29 based on the auc. in acute systemic infections in ... | 2001 | 11502533 |
| from the centers for disease control and prevention. outbreak of listeriosis associated with homemade mexican-style cheese--north carolina, october 2000-january 2001. | 2001 | 11508310 | |
| development of a proposed standard method for assessing the efficacy of fresh produce sanitizers. | a series of studies was done for the purpose of developing a proposed standard method to evaluate point-of-use home sanitizers for fresh produce. preliminary experiments were done to determine the survival of escherichia coli o157:h7, salmonella, and listeria monocytogenes after inoculation onto the surface of ripe tomatoes and drying for up to 24 h at 22 +/- 2 degrees c. within 2 h, the initial population (6.88 log10 cfu/tomato) of e. coli o157:h7 was reduced by approximately 3 log10, while red ... | 2001 | 11510643 |
| analysis of native microflora and selection of strains antagonistic to human pathogens on fresh produce. | the native microflora of three types of produce (green bell peppers, romaine lettuce, and prepeeled baby carrots) and two types of sprouting seeds (alfalfa and clover) were investigated. aerobic plate count (apc) for each produce or seed type as determined on pseudomonas agar f (paf) with incubation at 28 degrees c was in the range of 4 to 7 log cfu per g of tissue or seed. there was no significant difference (p > or = 0.05) in apc when the determinations were made with three agar media includin ... | 2001 | 11510644 |
| pathogen testing of ready-to-eat meat and poultry products collected at federally inspected establishments in the united states, 1990 to 1999. | the food safety and inspection service (fsis) conducted microbiological testing programs for ready-to-eat (rte) meat and poultry products produced at approximately 1,800 federally inspected establishments. all samples were collected at production facilities and not at retail. we report results here for the years 1990 through 1999. prevalence data for salmonella, listeria monocytogenes, escherichia coli o157:h7, or staphylococcal enterotoxins in nine different categories of rte meat and poultry p ... | 2001 | 11510658 |
| home-style beef jerky: effect of four preparation methods on consumer acceptability and pathogen inactivation. | the safety of homemade jerky continues to be questioned. producing a safe product that retains acceptable quality attributes is important. lethality of salmonella, escherichia coli o157:h7, and listeria monocytogenes as well as consumer acceptability and sensory attributes of jerky prepared by four methods were examined. preparation methods were drying marinated strips at 60 degrees c (representing a traditional method), boiling strips in marinade or heating in an oven to 71 degrees c prior to d ... | 2001 | 11510659 |
| antibacterial activity in extracts of camellia japonica l. petals and its application to a model food system. | the potential presence of naturally occurring antimicrobials in petals of camellia japonica l., a member of the tea family, was investigated against foodborne pathogens in microbiological media and food. petals of the camellia flower (c. japonica l.) were extracted with methanol and fractionated into basic, acidic, and neutral fractions. the acidic fraction (equivalent to 1.0 g of raw sample per disk) produced an inhibitory zone of 14 to 19 mm (diameter) in a disk assay against the pathogens sal ... | 2001 | 11510672 |
| differential role of cytosolic phospholipase a2 in the invasion of brain microvascular endothelial cells by escherichia coli and listeria monocytogenes. | invasion of brain microvascular endothelial cells (bmecs) is a key step in the pathogenesis of meningitis due to escherichia coli and listeria monocytogenes. although host cell actin cytoskeletal rearrangements are essential in bmec invasion by e. coli k1 and l. monocytogenes, the underlying signaling mechanisms remain unclear. this study demonstrates that host cell cytosolic phospholipase a2 (cpla2) contributes to e. coli k1 invasion of bmecs but not to l. monocytogenes invasion of bmecs. this ... | 2001 | 11517434 |
| listeriosis: an emerging food-borne disease. | listeriosis is a serious disease acquired by ingestion of foods contaminated with listeria monocytogenes. special populations at risk are individuals who are immunocompromised to some extent. the most common clinical findings include meningitis, septicemia, encephalitis, and intrauterine/cervical infections. food related listeriosis is an unusual occurrence but it is now being thought of as a new, and emergent illness. numerous types of food products have been implicated in listeriosis infection ... | 2001 | 11517630 |
| heat resistance and growth of salmonella enteritidis, listeria monocytogenes and aeromonas hydrophila in whole liquid egg. | salmonella enteritidis atcc,13067, listeria monocytogenes atcc,19116 and aeromonas hydrophila atcc,7965 strains were evaluated for growth and thermal resistance in liquid whole egg (lwe). each strain grew well in lwe at temperatures between 4 and 30 degrees c, except s. enteritidis which grew weakly at 4 and 10 degrees c. maximum populations for each strain increased with increasing growth temperature. the thermal destruction of each strain was determined in six liquid products. the egg products ... | 2001 | 11518391 |
| [less frequent etiology in uveitis]. | this material intends to present three clinical observations regarding the uveitis etiology, that is represented by the association of some etiological factors less known: toxoplasma gondii, listeria monocytogenes, leptospira icterohaemorrhagiae. the uveitis described here have been severe in the fast evolution and with trends of reappearence. this work demonstrates that the strict etiological antibacteria and antiparasite therapy is not enough and only corticotherapy is able to fight against th ... | 2001 | 11519339 |
| tnf-alpha hyper-responses to gram-negative and gram-positive bacteria in propionibacterium acnes primed or salmonella typhimurium infected mice. | ifn-gamma-dependent hypersensitivity to lps is inducible in mice by infection or pre-treatment with killed bacteria. hypersensitive mice exhibit enhanced inflammatory responses to lps, including the overproduction of tnf-alpha. using lps(n) balb/c and lps(d) balb/c/l mice, primed with propionibacterium acnes or infected with salmonella typhimurium, we show that concurrently to hypersensitivity to lps, a hypersensitivity to other constituents of killed gram-negative or gram-positive bacteria and ... | 2001 | 11521096 |
| calcitermin, a novel antimicrobial peptide isolated from human airway secretions. | the human airways are protected from pathogenic colonization by a blanket of fluid impregnated with innate antimicrobial effector molecules. among several previously uncharacterized components, we isolated a peptide that had activity primarily targeting gram-negative bacteria. we named the peptide 'calcitermin' since its amino acid sequence and mass were equivalent to the 15 c-terminal residues of the s100 protein, calgranulin c. the antimicrobial activity of calcitermin was enhanced in acidic b ... | 2001 | 11522286 |
| identification of a new plasmid-encoded sec-dependent bacteriocin produced by listeria innocua 743. | listeria innocua 743 produces an inhibitory activity demonstrating broad-spectrum inhibition of listeria monocytogenes isolates. gel-electrophoretic analysis of culture supernatants indicated that two inhibitors with different molecular weights were produced by this strain. insertion of tn917 into a 2.9 kb plasmid (phc743) generated mutants with either an impaired ability or a loss in ability to produce one of the inhibitors. sequence analysis of the transposon insertion regions revealed the pre ... | 2001 | 11526003 |
| farm-management and milking practices associated with the presence of listeria monocytogenes in new york state dairy herds. | knowledge of the relationship between dairy herd management and milking practices with the occurrence of listeria monocytogenes on dairy farms might assist in the development of intervention strategies aimed at eliminating this organism at the pre-harvest dairy-food-production level. this paper represents a first step towards that goal. we carried out a cross-sectional study to identify farm factors that were associated with isolation of l. monocytogenes from on-farm in-line milk-filters. data o ... | 2001 | 11530195 |
| molecular characterisation of aureocin a70, a multi-peptide bacteriocin isolated from staphylococcus aureus. | staphylococcus aureus a70 produces a heat-stable bacteriocin designated aureocin a70. aureocin a70 is encoded within a mobilisable 8 kb plasmid, prj6, and is active against listeria monocytogenes. experiments of transposition mutagenesis and gene cloning had shown that aureocin a70 production and immunity were associated with the hindiii-a and b fragments of prj6. therefore, a 6332 bp region of the plasmid, encompassing both these fragments, was sequenced using a concatenation dna sequencing pro ... | 2001 | 11531330 |
| [listeria monocytogenes meningitis in a patient with hiv infection and cytomegalovirus infection after colonoscopy]. | 2001 | 11534435 | |
| effect of exposure to diesel exhaust particles on the susceptibility of the lung to infection. | there are at least three mechanisms by which alveolar macrophages play a critical role in protecting the lung from bacterial or viral infections: production of inflammatory cytokines that recruit and activate lung phagocytes, production of antimicrobial reactive oxidant species, and production of interferon (an antiviral agent). in this article we summarize data concerning the effect of exposure to diesel exhaust particles on these alveolar macrophage functions and the role of adsorbed organic c ... | 2001 | 11544172 |
| positive selection of mutations leading to loss or reduction of transcriptional activity of prfa, the central regulator of listeria monocytogenes virulence. | transcription factor prfa controls the expression of virulence genes essential for listeria monocytogenes pathogenesis. to gain insight into the structure-function relationship of prfa, we devised a positive-selection system to isolate mutations reducing or abolishing transcriptional activity. the system is based on the observation that the listerial iap gene, encoding the p60 protein, is lethal if overexpressed in bacillus subtilis. a plasmid in which the iap gene is placed under the control of ... | 2001 | 11544218 |
| sterically stabilized cationic liposomes improve the uptake and immunostimulatory activity of cpg oligonucleotides. | immunostimulatory cpg oligonucleotides (odn) show promise as immune adjuvants, anti-allergens, and immunoprotective agents. increasing the bioavailability and duration of action of cpg odn should improve their therapeutic utility. encapsulating odn in sterically stabilized cationic liposomes provides protection from serum nucleases while facilitating uptake by b cells, dendritic cells, and macrophages. in a pathogen challenge model, sterically stabilized cationic liposomes encapsulation doubled ... | 2001 | 11544321 |
| assessment of the virulence of listeria monocytogenes: agreement between a plaque-forming assay with ht-29 cells and infection of immunocompetent mice. | some listeria monocytogenes strains not related to clinical cases have been found to exhibit a low virulence level in mice as well as in an in vitro test using caco-2 cells. the purpose of this study was to validate a new in vitro test of virulence based on a plaque-forming assay (pfa) using a ht-29 cell monolayer with 118 listeria strains. the use of ht-29 cells in 96-well tissue culture plates allowed the testing of 30 strains per day and providing results in 24 h. in addition. statistical ana ... | 2001 | 11545218 |
| comparison of the efficacy of different techniques, culture media, and sources of blood in determining the hemolytic activity of listeria spp.. | hemolytic activity is a fundamental criterion for the differentiation of listeria species; therefore, a simple and inexpensive procedure to clearly distinguish hemolytic strains from each other and from nonhemolytic strains would be of great aid. we compared the efficacy of several techniques, culture media, and types of blood in demonstrating the hemolysis of listeria spp. the hemolytic activities of listeria monocytogenes and listeria seeligeri were more easily detected with a red blood cell t ... | 2001 | 11547885 |
| [virulence factor expression regulation in listeria monocytogenes]. | the external and internal factors affecting the expression of l. monocytogenes virulence genes are analyzed. the role of protein prfa, the only known factor directly affecting the transcription of virulence genes, as well as the contribution of other regulatory elements modulating the activity and/or intracellular concentration of prfa are discussed. the environmental factors which influence the level of expression, such as temperature and the presence of metabolized carbohydrates in the cultiva ... | 2001 | 11550545 |
| [hemorrhagic syndrome during high-dose ampicillin treatment]. | in high doses, the beta-lactams--particularly cephalosporins--can cause bleeding from impairment of prothrombin formation. we report a case of hemorrhagic syndrome in a 63-year-old woman during therapy with high doses of ampicillin for listeria monocytogenes meningitis. | 2001 | 11552312 |
| fetal death in utero secondary to listeria monocytogenes placental infection. | 2001 | 11552741 | |
| eukaryotic expression plasmid transfer from the intracellular bacterium listeria monocytogenes to host cells. | the facultative intracellular, gram-positive bacterium listeria monocytogenes invades phagocytic and non-phagocytic cells from the tissues and organs of a wide variety of animals and humans. here, we report the use of these bacteria as vehicles for gene transfer. eukaryotic expression plasmids were introduced into the nucleus of host cells following lysis of the intracytosolic, plasmid-carrying bacteria with antibiotics. cell lines of different tissues and species could be transfected in this wa ... | 2001 | 11553012 |
| actin-based motility is sufficient for bacterial membrane protrusion formation and host cell uptake. | shigella flexneri replicates in the cytoplasm of host cells, where it nucleates host cell actin filaments at one pole of the bacterial cell to form a 'comet tail' that propels the bacterium through the host's cytoplasm. to determine whether the ability to move by actin-based motility is sufficient for subsequent formation of membrane-bound protrusions and intercellular spread, we conferred the ability to nucleate actin on a heterologous bacterium, escherichia coli. previous work has shown that i ... | 2001 | 11553015 |
| prevalence of listeria spp and listeria monocytogenes in meat and fermented fish in malaysia. | fermented fish and meat samples were purchased from supermarket and wet market for microbiological analysis of listeria species and listeria monocytogenes contamination. listeria species were isolated from 17 (73.9%) of 23 samples of imported frozen beef, 10 (43.5%) of the 23 samples of local beef and 14 (56%) of the 25 samples of fermented fish from wet market. listeria monocytogenes occurred in 15 (75%) of the frozen beef samples, 6 (30.4%) of the 23 samples of local meat and 3 (12%) of the 25 ... | 2001 | 11556596 |
| evidence for intraaxonal spread of listeria monocytogenes from the periphery to the central nervous system. | rhombencephalitis due to listeria monocytogenes is characterized by progressive cranial nerve palsies and subacute inflammation in the brain stem. in this paper, we report observations made on mice infected with l. monocytogenes. unilateral inoculation of bacteria into facial muscle, or peripheral parts of a cranial nerve, induced clinical and histological signs of mainly ipsilateral rhombencephalitis. similarly, unilateral inoculation of bacteria into lower leg muscle or peripheral parts of sci ... | 2001 | 11556688 |
| inactivation and injury of pressure-resistant strains of escherichia coli o157 and listeria monocytogenes in fruit juices. | to investigate methods for inactivating a pressure-resistant strain of escherichia coli o157 in fruit juices. | 2001 | 11556911 |
| factors affecting production of an antilisterial bacteriocin by carnobacterium piscicola strain a9b in laboratory media and model fish systems. | to investigate factors influencing bacteriocin production and bacteriocin stability of the bioprotective culture carnobacterium piscicola strain a9b. | 2001 | 11556917 |
| characterization of listeria monocytogenes isolated from production lines of fresh and cold-smoked fish. | the aims of this study were to characterize strains of listeria monocytogenes isolated from cold-smoking fish plants to establish possible routes of contamination through the processing chain. | 2001 | 11556924 |
| listeria monocytogenes septicaemia in foals. | 2001 | 11558750 | |
| the differential adherence capabilities of two listeria monocytogenes strains in monoculture and multispecies biofilms as a function of temperature. | to determine the differential adherence capabilities at three different temperatures of listeria monocytogenes scott a, a clinical food pathogen, and l. monocytogenes fm876, a persistent strain from a milk-processing environment, to stainless steel. | 2001 | 11559409 |
| regression of established human papillomavirus type 16 (hpv-16) immortalized tumors in vivo by vaccinia viruses expressing different forms of hpv-16 e7 correlates with enhanced cd8(+) t-cell responses that home to the tumor site. | using vaccinia virus as a live vector, we show that the expression of human papillomavirus type 16 (hpv-16) e7 fused to a nonhemolytic portion of the listeria monocytogenes virulence factor, listeriolysin o (llo), induces an immune response that causes the regression of established hpv-16 immortalized tumors in c57bl/6 mice. the vaccinia virus construct expressing llo fused to e7 (vaclloe7) was compared with two previously described vaccinia virus constructs: one that expresses unmodified e7 (va ... | 2001 | 11559797 |
| listeria protein acta mimics wasp family proteins: it activates filament barbed end branching by arp2/3 complex. | actin-based propulsion of the bacteria listeria and shigella mimics the forward movement of the leading edge of motile cells. while shigella harnesses the eukaryotic protein n-wasp to stimulate actin polymerization and filament branching through arp2/3 complex, the listeria surface protein acta directly activates arp2/3 complex by an unknown mechanism. here we show that the n-terminal domain of acta binds one actin monomer, in a profilin-like fashion, and arp2/3 complex and mimics the c-terminal ... | 2001 | 11560487 |
| reversible acute hydrocephalus complicating listeria monocytogenes meningitis. | 2001 | 11561813 | |
| protective effect of a traditional japanese medicine hochu-ekki-to (chinese name: bu-zhong-yi-qi-tang), on the susceptibility against listeria monocytogenes in infant mice. | in this study, the effect of traditional japanese (chinese) medicine, hochu-ekki-to, hot (chinese name: bu-zhong-yi-qi-tang), on the susceptibility against listeria monocytogenes in postneonatal infant mice was examined. numbers of bacteria in infant mice (infected at 4 weeks of age) were significantly higher than those in adult mice (infected at 8 weeks of age) on day 3 (non-specific resistance phase) and day 5 (specific resistance phase) after infection. oral administration of 1,000 mg/kg of h ... | 2001 | 11562059 |
| role of the glutamate decarboxylase acid resistance system in the survival of listeria monocytogenes lo28 in low ph foods. | the glutamate decarboxylase (gad) acid resistance system of the foodborne pathogen listeria monocytogenes plays a major role in its survival at low ph. it was found that survival of the wild-type strain. lo28, in acidified reconstituted skim milk, diluted to reduce free glutamate levels. improves in response to supplementation with monosodium glutamate. a mutant, in which the two listerial gad homologs have been deleted (and in which there is no discernible gad activity), did not respond to glut ... | 2001 | 11563513 |
| survival of listeria monocytogenes attached to stainless steel surfaces in the presence or absence of flavobacterium spp. | contaminated surfaces of food processing equipment are believed to be a significant source of listeria monocytogenes to foods. however, very little is known about the survival of listeria in processing environments. in a mixed bacterial biofilm of l. monocytogenes and flavobacterium spp., the number of l. monocytogenes cells attaching to stainless steel increased significantly compared to when l. monocytogenes was in a pure culture. the l. monocytogenes cells in the mixed biofilms were also reco ... | 2001 | 11563514 |
| monitoring of microbial hazards at farms, slaughterhouses, and processing lines of swine in korea. | this study was executed to investigate microbiological hazards at swine farms, slaughterhouses, dressing operations, and local markets for the application of the hazard analysis critical control point system in korea by analyzing total aerobic plate count (apc) and presence of pathogens. six integrated pig farms and meat packers were selected from six different provinces, and samples were collected from pig carcasses by swabbing and excision methods at the slaughterhouses, processing rooms, and ... | 2001 | 11563516 |
| combined effects of nacl, naoh, and biocides (monolaurin or lauric acid) on inactivation of listeria monocytogenes and pseudomonas spp. | this study highlighted combinations of chemical stresses that could decrease or eliminate listeria monocytogenes and pseudomonas spp. surviving in food processing plants. strains of l. monocytogenes, pseudomonas fragi, and pseudomonas fluorescens isolated from processing environments (meat and milk) were grown at 20 degrees c up to the early stationary phase. the strains were then subjected to 30 min of physicochemical treatments. these treatments included individual or combined acid (acetic aci ... | 2001 | 11563526 |
| the hazard analysis and critical control points (haccp) generic model for the production of thai fermented pork sausage (nham). | nham is a traditional thai fermented pork sausage. the major ingredients of nham are ground pork meat and shredded pork rind. nham has been reported to be contaminated with salmonella spp., staphylococcus aureus, and listeria monocytogenes. therefore, it is a potential cause of foodborne diseases for consumers. a hazard analysis and critical control points (haccp) generic model has been developed for the nham process. nham processing plants were observed and a generic flow diagram of nham proces ... | 2001 | 11570169 |
| feasibility of fluorescent detection of pathogens on pork carcasses. | the direct immunofluorescent detection of pathogens on pork skin is evaluated. calibrate contamination of pork skin with salmonella typhimurium (st) and listeria monocytogenes (lm) is developed in 2 h at 4 degrees c. then a specific indirect immunofluorescent staining protocol is optimized in order to obtain specific and intensive signals able to be detected by electronic cameras (deported microscopy). despite the individual staining of st and lm is possible on pork skin and is specific and brig ... | 2001 | 11570187 |
| role of sigma(b) in heat, ethanol, acid, and oxidative stress resistance and during carbon starvation in listeria monocytogenes. | to determine the contribution of sigma b (sigma(b)) to survival of stationary-phase listeria monocytogenes cells following exposure to environmental stresses, we compared the viability of strain 10403s with that of an isogenic nonpolar sigb null mutant strain after exposure to heat (50 degrees c), ethanol (16.5%), or acid (ph 2.5). strain viabilities were also determined under the same conditions in cultures that had been previously exposed to sublethal levels of the same stresses (45 degrees c, ... | 2001 | 11571142 |
| mutations in the listerial prob gene leading to proline overproduction: effects on salt tolerance and murine infection. | the observed sensitivity of listeria monocytogenes to the toxic proline analogue l-azetidine-2-carboxylic acid (az) suggested that proline synthesis in listeria may be regulated by feedback inhibition of gamma-glutamyl kinase (gk), the first enzyme of the proline biosynthesis pathway, encoded by the prob gene. taking advantage of the epicurian coli mutator strain xl1-red, we performed random mutagenesis of the recently described proba operon and generated three independent mutations in the liste ... | 2001 | 11571156 |
| internalin b activates nuclear factor-kappa b via ras, phosphoinositide 3-kinase, and akt. | internalin b (inlb), a 630-amino acid protein loosely attached to the surface of listeria monocytogenes, participates in the entry of the bacterium into mammalian cells. this process requires the activation of phosphoinositide (pi) 3-kinase by inlb. previously, we demonstrated that inlb activates the transcription factor nuclear factor-kappab in murine j774 macrophage-like cells, an event that also requires pi 3-kinase. here we have further investigated this phenomenon. inlb activated the small ... | 2001 | 11571285 |
| a role for cofilin and lim kinase in listeria-induced phagocytosis. | the pathogenic bacterium listeria monocytogenes is able to invade nonphagocytic cells, an essential feature for its pathogenicity. this induced phagocytosis process requires tightly regulated steps of actin polymerization and depolymerization. here, we investigated how interactions of the invasion protein inlb with mammalian cells control the cytoskeleton during listeria internalization. by fluorescence microscopy and transfection experiments, we show that the actin-nucleating arp2/3 complex, th ... | 2001 | 11571311 |
| microinjection and growth of bacteria in the cytosol of mammalian host cells. | most facultative intracellular bacteria replicate in specialized phagosomes after being taken up by mammalian cells. relatively few intracellular bacteria escape the phagosomal compartment with the help of cytolytic (pore-forming) proteins and replicate in the host cell cytosol. without such toxins, intracellular bacteria cannot reach this cellular compartment. to circumvent the requirement of an "escape" step, we developed a procedure allowing the efficient direct injection of bacteria into the ... | 2001 | 11572936 |
| internalins from the human pathogen listeria monocytogenes combine three distinct folds into a contiguous internalin domain. | listeria monocytogenes is an opportunistic, food-borne human and animal pathogen. host cell invasion requires the action of the internalins a (inla) and b (inlb), which are members of a family of listerial cell-surface proteins. common to these proteins are three distinctive n-terminal domains that have been shown to direct host cell-specific invasion for inla and inlb. here, we present the high-resolution crystal structures of these domains present in inlb and inlh, and show that they constitut ... | 2001 | 11575932 |
| adsorption, attachment and biofilm formation among isolates of listeria monocytogenes using model conditions. | to determine whether isolates of listeria monocytogenes differ in their ability to adsorb and form biofilms on a food-grade stainless steel surface. | 2001 | 11576310 |
| functional assembly of two membrane-binding domains in listeriolysin o, the cytolysin of listeria monocytogenes. | listeriolysin o (llo) is a major virulence factor secreted by the pathogenic listeria monocytogenes and acts as pore-forming cytolysin. based on sequence similarities between llo and perfringolysin (pfo), the cytolysin from clostridium perfringens of known crystallographic structure, two truncated llo proteins were produced: llo-d123, comprising the first three predicted domains, and llo-d4, the last c-terminal domain. the two proteins were efficiently secreted into the culture supernatant of l. ... | 2001 | 11577147 |
| relationship between nucleic acid ratios and growth in listeria monocytogenes. | listeria monocytogenes is a pathogen whose distribution in a range of foodstuffs requires the development of methods for sensitive and rapid detection. molecular biological methods usually rely on specific detection of l. monocytogenes rdna directly amplified by the application of pcr to dna extracts. information on the metabolic status of l. monocytogenes populations would be valuable and can, in theory, be provided by quantitative detection of rrna itself. both fluorometry and oligonucleotide ... | 2001 | 11577148 |
| pivotal role of vasp in arp2/3 complex-mediated actin nucleation, actin branch-formation, and listeria monocytogenes motility. | the listeria monocytogenes acta protein mediates actin-based motility by recruiting and stimulating the arp2/3 complex. in vitro, the actin monomer-binding region of acta is critical for stimulating arp2/3-dependent actin nucleation; however, this region is dispensable for actin-based motility in cells. here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (vasp) recruitment by acta can bypass defects in actin monomer-binding. furthermore, purified vasp enh ... | 2001 | 11581288 |
| difference in cholesterol-binding and cytolytic activities between listeriolysin o and seeligeriolysin o: a possible role of alanine residue in tryptophan-rich undecapeptide. | we have constructed recombinant listeriolysin o (rllo) and seeligeriolysin o (rlso) from listeria monocytogenes and listeria seeligeri, respectively. in hemolysis and cholesterol-binding assays, the specific activity of recombinant toxin was lower for lso as compared to llo. to understand the molecular basis of this difference, in particular with respect to the conserved trp-rich undecapeptide, a naturally occurring ala to phe substitution in lso was introduced into rllo. the rllo:a488f hemolysi ... | 2001 | 11583846 |
| detection of low levels of listeria monocytogenes within 20 hours using an evanescent wave biosensor. | 2001 | 11586938 | |
| inhibition of interleukin-12 production by auranofin, an anti-rheumatic gold compound, deviates cd4(+) t cells from the th1 to the th2 pathway. | 1. interleukin-12 (il-12) may play a central role in the development and progression of rheumatoid arthritis by driving the immune response towards t helper 1 (th1) type responses characterized by high ifn-gamma and low il-4 production. in this study we investigated the effect of auranofin (af), an anti-rheumatic gold compound, on il-12 production in mouse macrophages and dendritic cells, and studied whether af-mediated inhibition of il-12 production could regulate a cytokine profile of antigen ... | 2001 | 11588111 |
| intraoperative prostaglandin e1 improves antimicrobial and inflammatory responses in alveolar immune cells. | anesthesia and surgery decrease antimicrobial and increase proinflammatory functions of alveolar immune cells. thus, anti-inflammatory agents that do not further suppress antimicrobial functions are required. we tested the hypothesis that intraoperative prostaglandin e1 (pge1) suppresses proinflammatory responses and prevents the reduction in antimicrobial responses of alveolar immune cells. | 2001 | 11588457 |
| quantifying the relationship between multiple immunological parameters and host resistance: probing the limits of reductionism. | although reductionist experimental designs are excellent for identifying cells, molecules, or functions involved in resistance to particular microbes or cancer cells, they do not provide an integrated, quantitative view of immune function. in the present study, mice were treated with either dexamethasone (dex) or cyclosporin a (cya), and immune function and host resistance were evaluated. multivariate statistical methods were used to describe the relative importance of a broad range of immunolog ... | 2001 | 11591782 |
| the family of thiol-activated, cholesterol-binding cytolysins. | several species of both pathogenic and non-pathogenic grampositive bacteria within the genera streptococcus, clostridium and bacillus secrete cytolytic proteins that belong to a single, highly homologous family. the most widely known members of this family are streptolysin o, listeriolysin, perfringolysin, and pneumolysin. these toxins specifically require membrane cholesterol but, apparently, do not depend on any other specific cell surface receptor, so that they are able to lyse the cytoplasmi ... | 2001 | 11595631 |
| administration of superantigens protects mice from lethal listeria monocytogenes infection by enhancing cytotoxic t cells. | superantigens stimulate t-cell-receptor vbeta-selective t-cell proliferation accompanying the release of cytokines, which may eventually protect the host from microbial infections. we investigated here whether superantigens can rescue the host from lethal bacterial infection. mice were pretreated with staphylococcus aureus enterotoxin b (seb) 1 and 2 days before bacterial infection, and the mortality of infected mice was assessed. seb pretreatment protected mice from lethal infection with lister ... | 2001 | 11598032 |