Publications

the pore-forming protein aep1 is an innate immune molecule that prevents zebrafish from bacterial infection.	following the aeromonas hydrophila aerolysin, various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. we have recently reported the mechanism of receptor recognition and in vitro pore-formation of a zebrafish aerolysin-like protein dln1/aep1. however, the physiological function of aep1 remains unknown. here we detected that aep1 gene is constitutively expressed in various immune-related tissues of adult zebrafish; and moreover, its expression is significan ...	2018	29317232
production, characterization and valuable applications of exopolysaccharides from marine bacillus subtilis sh1.	exopolysaccharides (epss) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic. many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields. the present study attempt to optimize the production of eps by marine bacillus subtilis sh1 in addition to charac ...	2017	29319513
a novel gata-like zinc finger transcription factor involving in hematopoiesis of eriocheir sinensis.	gata transcription factor is a family of dna-binding proteins that can recognize and bind to sequence of (a/t) gata (a/g). in the present study, a gata-like protein (named as esglp) was characterized from eriocheir sinensis, including an 834 bp full length open reading frame of esglp, encoding a polypeptide of 277 amino acids. the deduced amino acid sequence of esglp contained one conserved gata-type zinc finger of the form cys-x2-cys-x17-cys-x2-cys, with four cysteine sites. the esglp mrna tran ...	2018	29325712
a systematic investigation on the composition, evolution and expression characteristics of chemokine superfamily in grass carp ctenopharyngodon idella.	chemokines are a superfamily of small cytokines and characterized based on their ability to induce directional migration of cells along a concentration gradient by binding to chemokine receptors, which have important roles in immunology and development. due to the numerous and diverse members, systematic identifications of chemokine superfamily genes are difficult in many species. to that end, a comprehensive analysis of blast and scripting language was conducted to systematically identify and c ...	2018	29325765
molecular characterization and expression of complement factor i in pelteobagrus vachellii during aeromonas hydrophila infection.	complement factor i (cfi) is a novel regulatory serine protease that plays an important role in resistance to pathogen infection. in this study, the cfi gene of pelteobagrus vachellii (pv-cfi) was sequenced and characterized. the full-length cdna of 2320 bp includes a 155 bp 5'-untranslated region (utr), a 164 bp 3'-utr, and a 2001 bp open reading frame (orf) encoding a 667 amino acids. multiple sequence alignment revealed five highly conserved domains with a typical modular architecture and ide ...	2018	29329952
nonspecific bacterial flora isolated from the body surface and inside ixodes ricinus ticks.	ixodes ricinus and other representatives of the order ixodida are vectors of typical pathogens: borrelia burgdorferi sensu lato, anaplasma phagocytophilium, babesia spp., a tick-borne encephalitis virus, and other microorganisms which are important from a medical and veterinary point of view. the presented study focuses on the verification of nonspecific bacterial flora of i. ricinus. we analyzed ticks collected in a forest region in silesia, an industrial district in poland. methods of classica ...	2012	29334060
deficiency of dietary pyridoxine disturbed the intestinal physical barrier function of young grass carp (ctenopharyngodon idella).	the aim of this study was to assess the effects of dietary pyridoxine (pn) deficiency on intestinal antioxidant capacity, cell apoptosis and intercellular tight junction in young grass carp (ctenopharyngodon idella). a total of 540 young grass carp (231.85 ± 0.63 g) were fed six diets containing graded levels of pn (0.12-7.48 mg/kg diet) for 10 weeks. at the end of the feeding trial, the fish were challenged with aeromonas hydrophila for 2 weeks. the results showed that compared with the optimal ...	2018	29339045
molecular characterization and function of β-n-acetylglucosaminidase from ridgetail white prawn exopalaemon carinicauda.	chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and β-n-acetylglucosaminidase (nagase). in this paper, the full-length cdna sequence encoding nagase (ecnag) was obtained from exopalaemon carinicauda. the deduced amino acid sequence of ecnag open reading frame (orf) contained one glycohydro_20b2 domain and one glyco_hydro_20 domain. based on the cdna sequence, the genomic structure of ecnag was characterized and it was composed of six exons and five intron ...	2018	29339067
expression and functional characterization of transferrin in nile tilapia (oreochromis niloticus) in response to bacterial infection.	transferrin (tf), an iron-binding glycoprotein, plays an important role in host defense against pathogenic infection, which inhibits the growth and proliferation of pathogens, deprives iron from invading pathogens, and activates anti-microbial responses in macrophages. in this study, a tf homologue (ontf) was identified from nile tilapia (oreochromis niloticus) and characterized at expression pattern against bacterial infection and capability binding bacterial pathogens. the open reading frame o ...	2018	29353079
the fight for invincibility: environmental stress response mechanisms and aeromonas hydrophila.	aeromonas hydrophila is a freshwater-dwelling zoonotic bacterium that has economic importance in aquaculture. in the past decade, aeromonas hydrophila has become increasingly important because of its emergence as a food-borne zoonotic pathogen that is resistant to different treatment regimes. being an aquatic bacterium, aeromonas hydrophila is frequently subjected to several stressful environmental conditions, including changes in temperature, acidic ph and starvation that challenge its survival ...	2018	29355702
complement component bf/c2b gene mediates immune responses against aeromonas hydrophila in grass carp ctenopharyngodon idella.	the complement system is a significant component of innate immunity. here, we identified a bf/c2 homolog (gcbf/c2b) in grass carp. gcbf/c2b shares a high similarity with bf/c2b counterparts in other teleosts. gcbf/c2b transcription was widely distributed in different tissues, induced by aeromonas hydrophila in vivo and in vitro, and affected by lipopolysaccharide and flagellin stimulation in vitro. in cells over-expressing gcbf/c2b, transcript levels of all components except gcc5 were significan ...	2018	29355764
proteomic analysis of alterations in aeromonas hydrophila outer membrane proteins in response to oxytetracycline stress.	in gram-negative bacteria, the outer membrane proteins (omps) perform a crucial role in antibiotic resistance, but it is largely unknown how they behave in response to antibiotic stress. in this study, we treated aeromonas hydrophila with two different doses of oxytetracycline (oxy) to induce antibiotic stress. proteins were isolated from sarcosine-insoluble fractions and quantitatively examined by using tandem mass tag labeling-based mass spectrometry to identify differentially expressed protei ...	2018	29356594