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isolation and characterization of bacillus cereus ist105 from electroplating effluent for detoxification of hexavalent chromium.electroplating industries are the main sources of heavy metals, chromium, nickel, lead, zinc, cadmium and copper. the highest concentrations of chromium (vi) in the effluent cause a direct hazards to human and animals. therefore, there is a need of an effective and affordable biotechnological solution for removal of chromium from electroplating effluent.201122351260
substituted salicylaldehydes as potential antimicrobial drugs: minimal inhibitory and microbicidal concentrations.substituted salicylaldehydes are potent antibacterial and antifungal agents and may have chemotherapeutic potential. in the clinical setting, the minimal inhibitory concentration (mic) as well as the minimal bactericidal and fungicidal concentrations (mbc and mfc, respectively) are of fundamental interest. therefore, we have now, using a panel of five microbial species (bacillus cereus, candida albicans, escherichia coli, saccharomyces cerevisiae, and staphylococcus aureus), determined the mic a ...201122351982
[investigation of ampicillin effect on morphological and mechanical properties of escherichia coli and bacillus cereus cells with atomic force microscopy].the effect of subbacteriostatic concentrations of ampicillin on morphological and mechanical properties of gramnegative and grampositive cells of escherichia coli k12 tg1 and bacillus cereus ip 5832 respectively was studied with atomic force microscopy. significant heterogeneity of the bacterial populations was shown by the character of the response to the antibiotic effect. the common feature was increase of the cell size likely due to the effect of the inner osmotic pressure on the lowered cel ...201122359862
sverdlovsk revisited: pulmonary pathology of inhalational anthrax versus anthraxlike bacillus cereus pneumonia. 201222372894
complete genome sequence of the highly hemolytic strain bacillus cereus f837/76.highly hemolytic strain bacillus cereus f837/76 was isolated in 1976 from a contaminated prostate wound. the complete nucleotide sequence of this strain reported here counts nearly 36,500 single-nucleotide differences from the closest sequenced strain, bacillus thuringiensis al hakam. f827/76 also contains a 10-kb plasmid that was not detected in the al hakam strain.201222374959
occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment.the occurrence of sulfonamide and tetracycline resistance and their pollution profile in the aquaculture environment of tianjin, northern china, were investigated. the presence of antibiotic-resistant bacteria was identified and the corresponding antibiotic resistance genes (args) were quantified at 6 aquaculture farms in tianjin. sulfonamide-resistance genes were prevalent and their concentrations were the highest detected (3.0 × 10(-5) to 3.3 × 10(-4) for sul1/16s rdna, 2.0 × 10(-4) to 1.8 × 1 ...201222377146
x-ray absorption spectroscopy of metal site speciation in the metallo-β-lactamase bcii from bacillus cereus.cobalt and zinc binding by the subclass b1 metallo-β-lactamase bcii from bacillus cereus is examined by x-ray absorption spectroscopy, at various levels of metal loading. the data show that a significant amount of the dinuclear enzyme is formed, even at substoichiometric levels of metal loading, whether the added metal is zn(ii) or co(ii). increasing metal addition, from 0.5 to 1.0 to 2.0eq/mol of enzyme, are shown to result in a more ordered active site. while zn(ii) appears to show no preferen ...201222381913
inhibition of cytotoxicity by the nhe cytotoxin of bacillus cereus through the interaction of dodecyl maltoside with the nheb component.nhe ('nonhaemolytic enterotoxin') is a three-component cytotoxin implicated in the pathogenesis of diarrhoea by bacillus cereus. nhe forms pores in pure lipid bilayers, but the function of the individual components (nhea, nheb and nhec) remains unclear. nheb and nhec are structural homologues of clya, a pore-forming cytotoxin of escherichia coli. the non-ionic detergent dodecyl maltoside (ddm) has been shown to inhibit haemolysis of clya. we used ddm as a probe to examine the response of the nhe ...201222385099
interactive and single effects of ectomycorrhiza formation and bacillus cereus on metallothionein mt1 expression and phytoextraction of cd and zn by willows.single and joint ectomycorrhizal (+ hebeloma mesophaeum) and bacterial (+ bacillus cereus) inoculations of willows (salix viminalis) were investigated for their potential and mode of action in the promotion of cadmium (cd) and zinc (zn) phytoextraction. dual fungal and bacterial inoculations promoted the biomass production of willows in contaminated soil. single inoculations either had no effect on the plant growth or inhibited it. all inoculated willows showed increased concentrations of nutrit ...201122389535
molecular typing in public health laboratories: from an academic indulgence to an infection control imperative.using three austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on dna subtyping. dna macrorestriction analysis by pulsed field gel electrophoresis has become the gold standard for subtyping of food borne pathogens like listeria, salmonella, campylobacter and bacillus cereus. using a salmonella mbandaka outbreak from the year 2010 as example, it is shown how the comp ...201222389752
detection and expression of enterotoxin genes in endophytic strains of bacillus cereus.the aim of this study was to determine whether endophytic bacillus cereus isolates from agronomic crops possessed genes for the nonhaemolytic enterotoxin (nhe) and haemolysin bl (hbl) and, therefore, have the potential to cause diarrhoeal illness in humans.201222394121
prevalence, phenotypic traits and molecular characterization of emetic toxin-producing bacillus cereus strains isolated from human stools in korea.to investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing bacillus cereus strains isolated from sporadic food poisoning cases in korea.201222394210
antimicrobial and safety properties of lactobacilli isolated from two cameroonian traditional fermented foods.twenty-one lactobacillus isolates from "sha'a" (a maize - based fermented beverage) and "kossam" (traditionally fermented cow milk) were selected in accordance with their antagonistic activities and tested for their bacteriocinogenic potential as well as safety properties. these isolates were preliminarily identified as lactobacillus plantarum (62%), lactobacillus rhamnosus (24%), lactobacillus fermentum (10%) and lactobacillus coprophilus (4%) based on phenotypic characteristics and rep-pcr gen ...201122396914
semisynthesis and antimicrobial activity of novel guttiferone-a derivatives.six derivatives of guttiferone-a (lfqm-79, 80, 81, 82, 113 and 114) were synthesized and evaluated for their antimicrobial activity against the opportunistic or pathogenic fungi candida albicans (atcc 09548), candida glabrata (atcc 90030), candida krusei (atcc 6258), candida parapsilosis (atcc 69548), candida tropicalis (atcc 750), cryptococcus neoformans (atcc 90012), trichophyton tonsurans, microsporum gypseum and also against the opportunistic and pathogenic gram-positive bacteria staphylococ ...201222401914
psychrotolerant paenibacillus tundrae isolates from barley grains produce new cereulide-like depsipeptides (paenilide and homopaenilide) that are highly toxic to mammalian cells.paenilide is a novel, heat-stable peptide toxin from paenibacillus tundrae, which colonizes barley. p. tundrae produced 20 to 50 ng of the toxin mg(-1) of cells (wet weight) throughout a range of growth temperatures from +5°c to +28°c. paenilide consisted of two substances of 1,152 da and 1,166 da, with masses and tandem mass spectra identical to those of cereulide and a cereulide homolog, respectively, produced by bacillus cereus ns-58. the two components of paenilide were separated from those ...201222407690
molecular and toxigenic characterization of bacillus cereus and bacillus thuringiensis strains isolated from commercial ground roasted coffee.thirty samples of roasted ground coffee beans from 10 different commercial brands were analyzed to investigate the occurrence and levels of bacillus cereus and bacillus thuringiensis strains. strains were evaluated for their genetic diversity by repetitive element sequence polymorphism pcr (rep-pcr) and for their toxigenic profiles, i.e., the presence of hbla, hblc, hbld, nhea, nheb, nhec, cytk, ces, and entfm. survival and multiplication of b. cereus sensu lato in the ready-to-drink coffee was ...201222410226
characterization of lysb4, an endolysin from the bacillus cereus-infecting bacteriophage b4.bacillus cereus is a foodborne pathogen that causes emetic or diarrheal types of food poisoning. the incidence of b. cereus food poisoning has been gradually increasing over the past few years, therefore, biocontrol agents effective against b. cereus need to be developed. endolysins are phage-encoded bacterial peptidoglycan hydrolases and have received considerable attention as promising antibacterial agents.201222416675
antiproliferative, antimicrobial and apoptosis inducing effects of compounds isolated from inula viscosa.the antiproliferative and antimicrobial effects of thirteen compounds isolated from inula viscosa (l.) were tested in this study. the antiproliferative activity was tested against three cell lines using the mtt assay. the microdilution method was used to study the antimicrobial activity against two gram positive bacteria, two gram negative bacteria and one fungus. the apoptotic activity was determined using a tunel colorimetric assay. scanning electron microscopy was used to study the morphologi ...201222418930
spectroscopic studies of the iron and manganese reconstituted tyrosyl radical in bacillus cereus ribonucleotide reductase r2 protein.ribonucleotide reductase (rnr) catalyzes the rate limiting step in dna synthesis where ribonucleotides are reduced to the corresponding deoxyribonucleotides. class ib rnrs consist of two homodimeric subunits: r1e, which houses the active site; and r2f, which contains a metallo cofactor and a tyrosyl radical that initiates the ribonucleotide reduction reaction. we studied the r2f subunit of b. cereus reconstituted with iron or alternatively with manganese ions, then subsequently reacted with mole ...201222432022
'candidatus arthromitus' revised: segmented filamentous bacteria in arthropod guts are members of lachnospiraceae.the name arthromitus has been applied collectively to conspicuous filamentous bacteria found in the hindguts of termites and other arthropods. first observed by joseph leidy in 1849, the identity of these filaments has remained contentious. while margulis and colleagues declared them to be a life stage of bacillus cereus, others have assumed them to belong to the same lineage as the segmented filamentous bacteria (sfb) from vertebrate guts, a group that has garnered much attention due to their u ...201222436008
impact of intestinal microbiota and gastrointestinal conditions on the in vitro survival and growth of bacillus cereus.ingestion of b. cereus can result in diarrhea, if these bacteria survive gastrointestinal passage and achieve growth and enterotoxin production in the small intestine. the gastrointestinal survival of vegetative cells and spores of the diarrheal food poisoning strain b. cereus nvh 1230-88 was investigated during in vitro batch experiments simulating the stomach, duodenum and ileum using simulation media and competing intestinal microbiota. all spores and approx. 30% of the vegetative b. cereus c ...201222436640
structural analysis of the leptospiral sphingomyelinases: in silico and experimental evaluation of sph2 as an mg-dependent sphingomyelinase.leptospiral sphingomyelinases are candidate virulence factors present only in pathogenic leptospira spp. leptospira interrogans serovar lai encodes sph1, sph2, sph3, sph4 and sphh. except for sph4, they all possess the exo-endo-phosphatase domain that groups them under the dnase i superfamily.201222441407
a comparative study of the bactericidal activity and daily disinfection housekeeping surfaces by a new portable pulsed uv radiation device.daily cleaning and disinfecting of non-critical surfaces in the patient-care areas are known to reduce the occurrence of health care-associated infections. however, the conventional means for decontamination of housekeeping surfaces of sites of frequent hand contact such as manual disinfection using ethanol wipes are laborious and time-consuming in daily practice. this study evaluated a newly developed portable pulsed ultraviolet (uv) radiation device for its bactericidal activity in comparison ...201222447288
characterization of zinc-solubilizing bacillus isolates and their potential to influence zinc assimilation in soybean seeds.one hundred thirty-four putative bacillus isolates were recovered from soybean rhizosphere soils of nimar region to select effective zinc solubilizers for increased assimilation of zinc (zn) in soybean seeds. these isolates were screened in vitro for zinc-solubilization ability on tris-minimal agar medium supplemented separately with 0.1% zinc in the form of zinc oxide, zinc phosphate, and zinc carbonate. of all, 9 isolates and a reference bacillus cereus atcc 13061 were characterized and identi ...201222450791
characterization of a spore-specific protein of the bacillus cereus group.bc1245 is a monocistronic chromosomal gene of bacillus cereus atcc 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in b. cereus. bc1245 is highly conserved in the genome of members of the b. cereus group, indicating an important function of the gene in this group of bacteria. quantitative pcr revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell-specifi ...201222458449
characterization of two metal resistant bacillus strains isolated from slag disposal site at burnpur, india.two strains of bacillus sp. resistant to arsenate and lead designated as assp9 and pbsp6, respectively were isolated from the slag disposal site. they were identified to be related to bacillus cereus cluster on the basis of 16s rdna based sequence analysis and phenotypic characteristics. both were rod-shaped (assp9, 2-5 microm and pbsp6, 2-4 microm), aerobic, salt tolerant (2-8% naci), endospore forming bacteria with minor differences like the assp9 showed sporangial bulging and pbsp6 had positi ...201122471215
bacteriophages bcp1-1 and bcp8-2 require divalent cations for efficient control of bacillus cereus in fermented foods.bacillus cereus is a foodborne bacterial pathogen that causes diarrhea and vomiting. in this study, the usefulness of bacteriophages to eradicate b. cereus from fermented foods was investigated. a total of 13 phages were isolated from korean fermented food products, and 2 (bcp1-1 and bcp8-2) were further characterized. transmission electron microscopy (tem), restriction enzyme digestion pattern analysis, and sds-page of the structural proteins suggest that both phages belong to the family myovir ...201222475937
bacterial contamination of an automated pharmacy robot used for intravenous medication preparation.multiple cultures were positive for bacillus cereus during routine quality assurance testing of a pharmacy robot that prepares intravenous medications. an investigation confirmed bacterial contamination of the robot as well as drug product made by the robot. the process and outcomes of the investigation are described in this report.201222476280
oligo-dna custom macroarray for monitoring major pathogenic and non-pathogenic fungi and bacteria in the phyllosphere of apple trees.to monitor the richness in microbial inhabitants in the phyllosphere of apple trees cultivated under various cultural and environmental conditions, we developed an oligo-dna macroarray for major pathogenic and non-pathogenic fungi and bacteria inhabiting the phyllosphere of apple trees.201222479577
phyto-synthesis of silver nanoscale particles using morinda citrifolia l. and its inhibitory activity against human pathogens.leaf extract of morinda citrifolia l. was assessed for the synthesis of silver nanoscale particles under different temperature and reaction time. synthesized nanoscale (mcagnps) particles were confirmed by analysing the excitation of surface plasmon resonance (spr) using uv-visible spectrophotometer at 420 nm. further sem, hrtem analysis confirmed the range of particle size between 10 and 60 nm and sead pattern authorizes the face centered cubic (fcc) crystalline nature of the mcagnps. fourier t ...201222483838
[antibacterial activity of rare streptomyces species against clinical resistant bacteria].in the search for new antibiotics from steptomyces, investigating extremes habitats enhances the probability of isolating novel producers. in this context, the antibacterial activity of four streptomyces strains isolated from ezzmoul saltpans was studied. two of them showed antibacterial activity against antibiotic's resistant bacteria (bacillus cereus: β-lactamines and sulfamides resistant, streptococcus faecalis: penicillin, tetracycline and cotrimoxazole resistant, and staphylococcus aureus m ...201222484527
survival of bacillus cereus vegetative cells and spores during in vitro simulation of gastric passage.the enteric pathogen bacillus cereus must survive gastric passage in order to cause diarrhea by enterotoxin production in the small intestine. the acid resistance and the survival after gastric passage were assessed by in vitro experiments with acidified growth medium and gastric simulation medium with b. cereus nvh 1230-88 vegetative cells and spores. first, batch incubations at constant ph values for 4 h, which represented different physiological states of the stomach, showed that spores were ...201222488056
antimicrobial effects of silver nanoparticles against bacterial cells adhered to stainless steel surfaces.given the increasing number of antibiotic-resistant bacteria and the need to synthesize new antimicrobials, silver has attracted interest in the scientific community because of its recognized antimicrobial activity. this study aimed to evaluate the antimicrobial effects of silver nanoparticles (np) obtained by a new method and tested at concentrations of 6 μg/ml and 60 μg/ml against the species staphylococcus aureus, listeria innocua, salmonella choleraesuis, pseudomonas aeruginosa, escherichia ...201222488058
microbiological performance of a food safety management system in a food service operation.the microbiological performance of a food safety management system in a food service operation was measured using a microbiological assessment scheme as a vertical sampling plan throughout the production process, from raw materials to final product. the assessment scheme can give insight into the microbiological contamination and the variability of a production process and pinpoint bottlenecks in the food safety management system. three production processes were evaluated: a high-risk sandwich p ...201222488059
[screening and identification of a microcystin-degrading bacterium strain and its enzymatic degradation of microcystin-lr by intracellular extract of bacillus cereus].to provide effective microorganisms for the treatment of water polluted by microcystins (mcs), the strains capable of biodegrading microcystin lr (mc-lr) were isolated from the sediment of chaohu lake. the degradation of microcystin-lr by the intracellular extracts of the strains were studied.201222489466
invariant gly residue is important for α-defensin folding, dimerization, and function: a case study of the human neutrophil α-defensin hnp1.the human α-defensins (hnp) are synthesized in vivo as inactive prodefensins, and contain a conserved glycine, gly(17), which is part of a β-bulge structure. it had previously been shown that the glycine main chain torsion angles are in a d-configuration, and that d-amino acids but not l-alanine could be substituted at that position to yield correctly folded peptides without the help of a prodomain. in this study, the glycine to l-alanine mutant defensin was synthesized in the form of a prodefen ...201222496447
microbiological and physicochemical characterisation of caxiri, an alcoholic beverage produced by the indigenous juruna people of brazil.caxiri is a traditional fermented alcoholic beverage produced from cassava and sweet potatoes by the indigenous juruna or yudjá people in brazil. our results showed that caxiri fermentation is invariably associated with the following: (i) an increase in the total microbial population, with yeast being the largest group detected; (ii) a decrease in reducing sugars, malic, tartaric, succinic, oxalic and propionic acid; and (iii) a final product characterised by a high content of ethanol and a high ...201222497838
laccase- and chloroperoxidase-nanotube paint composites with bactericidal and sporicidal activity.laccase and chloroperoxidase (cpo) were separately immobilized onto multi-walled carbon nanotubes (mwnts) and subsequently mixed with a commercial eco-friendly paint to generate biocatalytic coatings. the laccase-nanotube based paints showed >99% bactericidal activity against escherichia coli and staphylococcus aureus (both challenged with 10⁶ cfu/ml) within 30 min and >98% sporicidal activity against bacillus cereus and bacillus anthracis-δsterne (initially challenged with 10⁴ cfu/ml) within 12 ...201222500892
inactivation kinetics of spores of bacillus cereus strains treated by a peracetic acid-based disinfectant at different concentrations and temperatures.the purpose of this study was to assess the effect of a commercial peracetic acid-based disinfectant against spores of bacillus cereus, to identify the most influential factor for the final number of microorganisms after different disinfection procedures, and to evaluate the nature of the inactivation kinetics. the spores of four different strains of b. cereus (dsm 318, 4312, 4313, and 4384) were treated with five different disinfectant concentrations (0.25%, 0.5%, 1.0%, 1.5%, and 2.0% [w/v]) at ...201222506696
biosecurity aspects and pathogen inactivation in acidified high risk animal by-products.the aim of the study was to investigate the effects of formic acid addition to ground high risk animal by-products (abp 1) in terms of stabilization and pathogen inactivation and to evaluate the biosecurity risk connected with the abp 1 based combustion fuel biomal. laboratory studies were performed on the persistence of salmonella typhimurium, bacillus cereus spores, porcine herpes virus, avian influenza virus, bovine viral diarrhea virus, equine rhinitis a virus and porcine parvovirus in bioma ...201222506709
foodborne outbreaks surveillance in the piedmont region, italy (2002-2009).this article summarises the results of eight years (2002-2009) of investigations on food borne disease outbreaks carried out the regional agency for public health in piedmont, which is an italian region (4,432,571 citizens, 2008).201222508645
partitioning studies of l-glutaminase production by bacillus cereus mtcc 1305 in different peg-salt/dextran.partitioning studies of l-glutaminase production by bacillus cereus mtcc 1305 was carried out in different peg-salt/peg-dextran system. the partitioning value of l-glutaminase increased with increasing molecular weight of peg from 2000-4000 kda and decreased with higher molecular weight of 6000 kda. phase system of peg 4000 (8.5%)/dextran t500 (9.5%) was selected for the extractive fermentative production of l-glutaminase on the basis maximum partition coefficient (1.31). the production of l-glu ...201222513254
synthesis of benzimidazolyl-1,3,4-oxadiazol-2ylthio-n-phenyl (benzothiazolyl) acetamides as antibacterial, antifungal and antituberculosis agents.to affiliate multiple bioactivities in a compact heteronuclei, two series of benzimidazole based 1,3,4-oxadiazoles were synthesized and assessed in vitro for their efficacy as antimicrobial agents against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneri), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberc ...201222516426
development of sandwich enzyme-linked immunosorbent assay for the detection of cronobacter muytjensii (formerly called enterobacter sakazakii).this study aimed to produce a polyclonal antibody against cronobacter muytjensii (c. muytjensii, formerly called enterobacter sakazakii) and to develop an immunoassay for its detection. the optimum production of rabbit anti-c. muytjensii immunoglobulin g (igg) and chicken anti-c. muytjensii igy was reached in weeks 8 and 9, respectively. purification of rabbit anti-c. muytjensii igg from immunized rabbit sera was accomplished using the caprylic acid and ammonium sulfate precipitation method. as ...201222519814
iron regulates expression of bacillus cereus hemolysin ii via global regulator fur.the capacity of pathogens to respond to environmental signals, such as iron concentration, is key to bacterial survival and establishment of a successful infection. bacillus cereus is a widely distributed bacterium with distinct pathogenic properties. hemolysin ii (hlyii) is one of its pore-forming cytotoxins and has been shown to be involved in bacterial pathogenicity in a number of cell and animal models. unlike many other b. cereus pathogenicity factors, hlyii is not regulated by pleiotropic ...201222522892
characterization of an endolysin, lysbps13, from a bacillus cereus bacteriophage.use of bacteriophages as biocontrol agents is a promising tool for controlling pathogenic bacteria including antibiotic-resistant bacteria. not only bacteriophages but also endolysins, the peptidoglycan hydrolyzing enzymes encoded by bacteriophages, have high potential for applications as biocontrol agents against food-borne pathogens. in this study, a putative endolysin gene was identified in the genome of the bacteriophage bps13, which infects bacillus cereus. in silico analysis of this endoly ...201222524239
biochemical analysis and investigation on the prospective applications of alkaline protease from a bacillus cereus strain.proteases have prospective financial and environment-friendly applications; hence attention is focused currently on the finding of new protease producing microorganism so as to meet the requirements of industry. a thermophilic bacterial strain producing extracellular protease activity was isolated from soil and identified as bacillus cereus by analysis of 16s rrna. protease production by the microorganism was improved by studying the impact of the type of nitrogen and carbon source, fermentation ...201222528469
on the active site of mononuclear b1 metallo β-lactamases: a computational study.metallo-β-lactamases (mβls) are zn(ii)-based bacterial enzymes that hydrolyze β-lactam antibiotics, hampering their beneficial effects. in the most relevant subclass (b1), x-ray crystallography studies on the enzyme from bacillus cereus point to either two zinc ions in two metal sites (the so-called '3h' and 'dch' sites) or a single zn(ii) ion in the 3h site, where the ion is coordinated by asp120, cys221 and his263 residues. however, spectroscopic studies on the b1 enzyme from b. cereus in the ...201222532071
trace detection of meglumine and diatrizoate from bacillus spore samples using liquid chromatography/mass spectrometry.following the september 11, 2001 terrorist attacks, letters containing bacillus anthracis were distributed through the united states postal system killing five people. a complex forensic investigation commenced to identify the perpetrator of these mailings. a novel liquid chromatography/mass spectrometry protocol for the qualitative detection of trace levels of meglumine and diatrizoate in dried spore preparations of b. anthracis was developed. meglumine and diatrizoate are components of radiogr ...201222537353
global mrna decay analysis at single nucleotide resolution reveals segmental and positional degradation patterns in a gram-positive bacterium.recent years have shown a marked increase in the use of next-generation sequencing technologies for quantification of gene expression (rna sequencing, rna-seq). the expression level of a gene is a function of both its rate of transcription and rna decay, and the influence of mrna decay rates on gene expression in genome-wide studies of gram-positive bacteria is under-investigated.201222537947
cody, a pleiotropic regulator, influences multicellular behaviour and efficient production of virulence factors in bacillus cereus.in response to nutrient limitation in the environment, the global transcriptional regulator cody modulates various pathways in low g+c gram-positive bacteria. in bacillus subtilis cody triggers adaptation to starvation by secretion of proteases coupled to the expression of amino acid transporters. furthermore, it is involved in modulating survival strategies like sporulation, motility, biofilm formation, and cody is also known to affect virulence factor production in pathogenic bacteria. in this ...201222540344
two piperazic acid-containing cyclic hexapeptides from streptomyces alboflavus 313.two novel cyclic hexapeptides, designated nw-g10 (1) and nw-g11 (2), were isolated from the fermentation broth of streptomyces alboflavus 313. their relative structures were elucidated on the basis of extensive spectroscopic analysis, and the absolute configurations of several constituent amino acids were determined by marfey's method. nw-g10 (1) and nw-g11 (2) exhibited significant activity against gram-positive bacteria, such as bacillus cereus, bacillus subtilis and staphylococcus aureus, inc ...201222543750
antibacterial anthraquinone derivatives from a sea anemone-derived fungus nigrospora sp.chemical investigation of a marine-derived fungus nigrospora sp., isolated from an unidentified sea anemone, yielded two new hydroanthraquinone analogues, 4a-epi-9α-methoxydihydrodeoxybostrycin (1) and 10-deoxybostrycin (2), together with seven known anthraquinone derivatives (3-9). the structures of the two new compounds were established through extensive nmr spectroscopy as well as a single-crystal x-ray diffraction analysis using cu kα radiation. the antibacterial activities of compounds 1-9 ...201222545792
development of metal-chelating inhibitors for the class ii fructose 1,6-bisphosphate (fbp) aldolase.it has long been suggested that the essential and ubiquitous enzyme fructose 1,6-bisphosphate (fbp) aldolase could be a good drug target against bacteria and fungi, since lower organisms possess a metal-dependant (class ii) fbp aldolase, as opposed to higher organisms which possess a schiff-base forming (class i) fbp aldolase. we have tested the capacity of derivatives of the metal-chelating compound dipicolinic acid (dpa), as well a thiol-containing compound, to inhibit purified recombinant cla ...201222546686
diversity of spore-forming bacteria and identification of bacillus amyloliquefaciens as a species frequently associated with the ropy spoilage of bread.this study examines the diversity of spore-forming bacteria isolated from raw materials/bread using molecular methods along with a rapid and innovative technology, the ft-nir spectroscopy. microbiological analysis showed that 23% of semolina and 42% of other raw materials (including grain, brewer yeast, improvers) contained more than 100 spores/g and more than 50% of each kind of sample was contaminated at a level ranging from 1 to 100 spores/g. a high bacterial diversity characterized raw mater ...201222551674
a urea channel from bacillus cereus reveals a novel hexameric structure.urea is exploited as a nitrogen source by bacteria, and its breakdown products, ammonia and bicarbonate, are employed to counteract stomach acidity in pathogens such as helicobacter pylori. uptake in the latter is mediated by urei, a uac (urea amide channel) family member. in the present paper, we describe the structure and function of uacbc, a homologue from bacillus cereus. the purified channel was found to be permeable not only to urea, but also to other small amides. cd and ir spectroscopy r ...201222553922
antimicrobial action of the leaf extract of moringa oleifera lam.the ethnolic extract of the leaves of moringa oleifera lam. (fam. moringaceae) was tested for antimicrobial activities against gram positive - bacillus cereus, bacillus subtilis staphylococcus aureus, sarcina lutea: gram negative - escherichia coli and acid fast mycobacterium phlei. significant antimicrobial activity of the extract was found in this study.199522556699
antimicrobial action of the leaf extract of jatropha tanjorensis (e&r).the ethalonic extract of the leaves of jatropha tanjorensis ellis & saroja was tested for antimicrobial activities against gram positive - bacillus cereus, bacillus subtilis, staphylococcus aureus, gram negative e. coli and mycobacterium pheli.199822556869
antimicrobial potency of the leaf - stalk extract of curcuma longa (linn).the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ...200022557005
antimicrobial activity of the mycotoxin citrinin obtained from the fungus penicillium citrinum.the mycotoxin citrinin was obtained from the fungus penicillium citrinum. it was tested for it's minimum inhibitory concentration (mic) against some gram positive strains viz. staphylococcus aureus, bacillus pumilus, bacillus subtillis, bacillus cereus, klebsiella pneumoniae, streptococcus pneumoniae, lactobacillus arabinosus and gram negative strains e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis and vibrio cholerae. further the zones o ...200222557053
antimicrobial action of the leaf extract of lagerstroemia parviflora roxb.the benzene extract of the leaves of lagerstroemia paviflora roxb was tested for its minimum inhibitory concentration (mic) against gram positive staphylococcus aureus, bacillus subtilis, bacillus cereus, klebsiella pneumoniae, streptococcus pneumoniae, lactobacillus arabinosus and gram negative strains e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis and vibrio cholerae. further the zones of inhibition produced by the crude extract agains ...200222557054
phytochemical and antimicrobial studies of extracts of solanum xanthocarpum.antibacterial activity pf various parts (stem, leaf and fruits) of solvent extracts (petroleum ether, alcohol and acetone) of solanum xanthocarpum against escherichia coli, klebsiella pneumoniae, salmonella typhi and bacillus cereus were detected by zone of inhibition. the extracts of solanum xanthocarpum showed high sensitivity to kiebsiella pneumoniae and salmonella typhi, moderate sensitivity to escherichia coli and less sensitivity and resistant to bacillus cereus. in control, there is no in ...200322557117
phytochemical and antimicrobial studies of extracts of solanum xanthocarpum.antibacterial activity of various parts (stem, leaf and fruits) of solvent extracts (petroleum ether, alcohol and acetone) of solanum xanthocarpum against escherichia coil, klebsiella of solanum xanthocarpum showed high sensitivity to escherichia coil & less sensitivity and resistance to bacillus cereus. in control, no inhibitory zone was observed.200422557158
pharmacognostic, phytochemical and anti-microbial studies of solanum indicum leaves.the leaves of solanum indicum showed the presence of stellate trichome. ethanolic extract of leaves solanum indicum showed antibacterial activity against staphylococcus aureus, bacillus cereus, escherichia coli where as chloroform extract, acetone extract and ethanol showed anti bacterial against pseudomonas. the aqueous extract did not show any anti microbial activity towards the tested organism. none of the extract showed anti fungal activity.200922557334
genome organization of temperate phage 11143 from emetic bacillus cereus nctc11143.a temperate phage was isolated from emetic bacillus cereus nctc 11143 by mitomycin c and characterized by transmission electron microscopy and dna and protein analyses. whole genome sequencing of bacillus phage 11143 was performed by gs-flx. the phage has a dsdna genome of 39,077 bp and a 35% g+c content. bioinformatic analysis of the phage genome revealed 49 putative orfs involved in replication, morphogenesis, dna packaging, lysogeny, and host lysis. bacillus phage 11143 could be classified as ...201222561859
solid-state fermentation: tool for bioremediation of adsorbed textile dyestuff on distillery industry waste-yeast biomass using isolated bacillus cereus strain ebt1.bioremediation of textile dyestuffs under solid-state fermentation (ssf) using industrial wastes as substrate pose an economically feasible, promising, and eco-friendly alternative. the purpose of this study was to adsorb red m5b dye, a sample of dyes mixture and a real textile effluent on distillery industry waste-yeast biomass (diw-yb) and its further bioremediation using bacillus cereus ebt1 under ssf. textile dyestuffs were allowed to adsorb on diw-yb. diw-yb adsorbed dyestuffs were decolori ...201322562346
antimicrobial activity of platelet (plt)-poor plasma, plt-rich plasma, plt gel, and solvent/detergent-treated plt lysate biomaterials against wound bacteria.platelet (plt) gels exhibit antimicrobial activity useful for wound healing. the nature of the antibacterial component(s) is unknown.201322563709
role of side-edge site of sphingomyelinase from bacillus cereus.bacillus cereus sphingomyelinase (bc-smase) belongs to the mg(2+)-dependent neutral sphingomyelinase (nsmase) which hydrolyzes sphingomyelin (sm) to produce phosphocholine and ceramide, and acts as an extracellular hemolysin. bc-smase has two metal ion-binding sites in a long horizontal cleft across the molecule, with one mg(2+) in the central region of the cleft and one divalent metal ion at the side-edge of the cleft. the role of the mg(2+) at the side-edge of the long horizontal cleft in bc-s ...201222564743
sequence analysis of inducible prophage phis3501 integrated into the haemolysin ii gene of bacillus thuringiensis var israelensis atcc35646.diarrheic food poisoning by bacteria of the bacillus cereus group is mostly due to several toxins encoded in the genomes. one of them, cytotoxin k, was recently identified as responsible for severe necrotic syndromes. cytotoxin k is similar to a class of proteins encoded by genes usually annotated as haemolysin ii (hlyii) in the majority of genomes of the b. cereus group. the partially sequenced genome of bacillus thuringiensis var israelensis atcc35646 contains several potentially induced proph ...201222567391
complete genome sequence of bacillus cereus bacteriophage pbc1.bacillus cereus is a ubiquitous, spore-forming bacterium associated with food poisoning cases. to develop an efficient biocontrol agent against b. cereus, we isolated lytic phage pbc1 and sequenced its genome. pbc1 showed a very low degree of homology to previously reported phages, implying that it is novel. here we report the complete genome sequence of pbc1 and describe major findings from our analysis.201222570248
characterization of boron resistant and accumulating bacteria lysinibacillus fusiformis m1, bacillus cereus m2, bacillus cereus m3, bacillus pumilus m4 isolated from former mining site, hokkaido, japan.boron is known to be widespread environmental contaminant that is relatively mobile in soil when compared to other metal contaminants. the present study made an attempt to isolate and characterize the boron resistant and accumulating bacteria from former mining site at hokkaido, japan. four potential strains m1, m2, m3 and m4 were selected based on high degree of boron and heavy metal resistances. the morphological, biochemical and 16s rdna sequencing analysis of mining bacteria revealed that th ...201222571522
cody orchestrates the expression of virulence determinants in emetic bacillus cereus by impacting key regulatory circuits.bacillus cereus causes gastrointestinal diseases and local and systemic infections elicited by the depsipeptide cereulide, enterotoxins, phospholipases, cytolysins and proteases. the plcr-papr quorum sensing system activates the expression of several virulence factors, whereas the spo0a-abrb regulatory circuit partially controls the plasmid-borne cereulide synthetase (ces) operon. here, we show that cody, a nutrient-responsive regulator of gram-positive bacteria, has a profound effect on both re ...201222571587
dimethyl disulfide is an induced systemic resistance elicitor produced by bacillus cereus c1l.bacillus cereus c1l is a plant growth-promoting rhizobacterium and can elicit induced systemic resistance (isr) in plants against necrotrophic pathogens. however, little is known about isr elicitors produced by b. cereus c1l, and no isr elicitor has been identified and characterised. therefore, the objective of this study is to identify volatile isr elicitor(s) produced by b. cereus c1l.201222573612
membrane interaction and antibacterial properties of chensinin-1, an antimicrobial peptide with atypical structural features from the skin of rana chensinensis.many antimicrobial peptides from amphibian skin have been purified and structurally characterized and may be developed as therapeutic agents. here we describe the antibacterial properties and membrane interaction of chensinin-1, a cationic arginine/histidine-rich antimicrobial peptide, from the skin secretions of rana chensinensis. the amino acid composition, sequence, and atypical structure of chensinin-1 differ from other known antimicrobial peptides from amphibian skin. chensinin-1 exhibited ...201222581068
investigations of antimicrobial activity of some cameroonian medicinal plant extracts against bacteria and yeast with gastrointestinal relevance.resistance against antibiotics of many bacteria is accumulating. therefore, searches for new substances with antimicrobial activity have become an urgent necessity. medicinal plants are frequently used in popular medicine as remedies for many infectious diseases (intestinal infection, malaria, tuberculosis, etc.).201222583961
rapid detection of bacterial contamination of platelet-rich plasma-derived platelet concentrates using flow cytometry.flow cytometry can be used to detect bacterial contamination of platelet products. in this study, we investigated whether the incubation of a minimal volume of platelet-rich plasma (prp)-derived platelet concentrates (pcs) with growth medium improved the analytical sensitivity of flow cytometry. five bacterial strains (staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, klebsiella pneumoniae, and escherichia coli) were used. platelets were inoculated with 10, 10(2), and 10(3) cfu ...201222585614
possible contamination of organ preservation fluid with bacillus cereus: the united kingdom response.we describe here the united kingdom (uk) response following the recent international recall of an organ preservation fluid owing to potential bacillus cereus contamination. this fluid is used for the transport of solid organs and pancreatic islet cells for transplant. we detail the response mechanisms, including the initial risk stratification, investigatory approaches, isolate analysis and communications to professional bodies. this report further lays out the potential need for enhanced survei ...201222587955
synthesis and antibacterial activities of 1-alkyl-3-methacryloyl (acryloyl) of benzimidazolone (thione) derivatives.a series of (28) 1-alkyl-3-methacryloyl (acryloyl)-benzimidazolone (thione) deriv-atives were synthesized. the structures of the new derivatives were confirmed by (1)h-nmr, (13)c-nmr and esi-ms spectral analysis. the antibacterial activities of these compounds against several strains of bacteria, such as bacillus cereus, bacillus subtilis, escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, were evaluated by methods of paper disc-diffusion and broth mciro-dilution. methacryloyl d ...201222606011
a multicomponent sugar phosphate sensor system specifically induced in bacillus cereus during infection of the insect gut.using a previously developed bacillus cereus in vivo expression technology (ivet) promoter trap system, we showed that spsa, a gene of unknown function, was specifically expressed in the larval gut during infection. search for gut-related compounds inducing spsa transcription identified glucose-6-phosphate (g6p) as an activation signal. analysis of the spsa-related 5-gene cluster indicated that spsa is part of a new sugar phosphate sensor system composed of a 2-component system (tcs) encoded by ...201222611084
bc4707 is a major facilitator superfamily multidrug resistance transport protein from bacillus cereus implicated in fluoroquinolone tolerance.transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen bacillus cereus that were up-regulated during stress produced by bile salts. one of these multidrug transporters (bc4707) was selected for investigation. functional characterization of the bc4707 protein in escherichia coli revealed a role in the energized efflux of xenobiotics. phenotypic analyses after inactivation of the gene bc4707 in bacillus cereus atcc14579 suggested a more spe ...201222615800
[analysis of microorganism species diversity in plant intercropping models in a wetland system constructed for treatment of municipal sewage].the selective culture method and pcr-dgge technology were applied to analyze the number and the biodiversity of microorganism species in cells with plant intercropping models and without plants in different seasons in a wetland system constructed for treatment of municipal sewage. the results showed that the numbers of microorganisms were considerably larger in the cells with plant intercropping models than those without plants, while the number of microorganisms was apparently larger in summer ...201122619969
novel polymer anchored cr(iii) schiff base complexes: synthesis, characterization and antimicrobial properties.new polymer-bound schiff bases and cr(iii) complexes have been synthesized by the reaction of 4-benzyloxybenzaldehyde, polymer-bound with 2-aminophenol, 2-amino-4-chlorophenol and 2-amino-4-methylphenol. the structure of polymeric-schiff bases and their cr(iii) complexes have been characterized by elemental analyses, magnetic measurements, ir, uv-vis, tg-dta and (1)h-nmr. all these compounds have also been investigated for antibacterial activity by the well-diffusion method against staphylococcu ...201222622060
synthesis of s-triazine-based thiazolidinones as antimicrobial agents.a novel series of thiazolidinone derivatives, namely 4-{4-dimethylamino-6-[4-oxo-2-phenyl-5-(4-pyridin-2-yl-piperazin-1-ylmethyl)-thiazolidin-3-yl]-[1,3,5]-triazin-2-yloxy}-1-methyl-1h-quinolin-2-ones, have been synthesized from the key intermediate 4-(4-amino-6-dimethylamino-[1,3,5]-triazin-2-yloxy)-1-methyl-1h-quinolin-2-one (5). compound 5 was condensed with various aldehydes to give schiff base derivatives, which after cyclization gave thiazolidinones that were linked with 1-pyridin-2-yl-pip ...201222624326
bacillus cereus septicemia attributed to a matched unrelated bone marrow transplant.hematopoietic cell transplantation (hct) is performed in more than 25,000 patients annually. clinically significant bacterial transmission from hct products is rare.201322624657
isopeptide bonds of the major pilin protein bcpa influence pilus structure and bundle formation on the surface of bacillus cereus.bacillus cereus strains elaborate pili on their surface using a mechanism of sortase-mediated cross-linking of major and minor pilus components. here we used a combination of electron microscopy and atomic force microscopy to visualize these structures. pili occur as single, double or higher order assemblies of filaments formed from monomers of the major pilin, bcpa, capped by the minor pilin, bcpb. previous studies demonstrated that within assembled pili, four domains of bcpa - cna(1), cna(2), ...201222624947
bacillus cereus endocarditis in native aortic valve.bacillus cereus endocarditis is rare. it has been implicated in immunocompromised individuals, especially in intravenous drug users as well as in those with a cardiac prosthesis. the patient was a 31-year-old ex-intravenous drug addict with a past history of staphylococcal pulmonary valve endocarditis, who presented with symptoms of decompensated cardiac failure. echocardiography showed severe aortic regurgitation with an oscillating vegetation seen on the right coronary cusp of the aortic valve ...201322627887
biologically active steroids from the aerial parts of vernonia anthelmintica willd.a new steroid, vernoanthelsterone a (1), and five known steroids were isolated from the aerial parts of vernonia anthelmintica willd. compound 1 possesses a δ(8(14))-15-one moiety. to our best knowledge, few steroids with this moiety have been reported before. compounds 1-6 were tested for their antibacterial activities and their effects on estrogen biosynthesis in human ovarian granulosa-like cells (kgn cells). compound 2 showed the ability to promote estrogen biosynthesis with ec(50) of 56.95 ...201222634578
interplay of rsbm and rsbk controls the σ(b) activity of bacillus cereus.the alternative transcription factor σ(b) of bacillus cereus controls the expression of a number of genes that respond to environmental stress. four proteins encoded in the sigb gene cluster, including rsbv, rsbw, rsby (rsbu) and rsbk, are known to be essential in the σ(b)-mediated stress response. in the context of stress, the hybrid sensor kinase rsbk is thought to phosphorylate the response regulator rsby, a pp2c serine phosphatase, leading to the dephosphorylation of the phosphorylated rsbv. ...201222640257
production of the antimicrobial peptides caseicin a and b by bacillus isolates growing on sodium caseinate.the aim of this study was to identify bacillus isolates capable of degrading sodium caseinate and subsequently to generate bioactive peptides with antimicrobial activity.201222642665
inhibition of macrophage migration by c. botulinum exoenzyme c3.c3-like exoenzymes are produced by various microorganism including clostridium botulinum (c3bot), bacillus cereus and staphylococcus aureus. c3bot is the prototype of c3-like exoenzymes that specifically adp-ribosylates and thereby inactivates rho(a/b/c). c3-like exoenzymes are not yet regarded as virulence factors, as the lack of cell entry domains results in a poor accessibility of the c3-like exoenzymes to cells. in this study, the sensitivity of various cell lines to c3bot has been reinvesti ...201222644106
genomic characterization of the bacillus cereus sensu lato species: backdrop to the evolution of bacillus anthracis.the key genes required for bacillus anthracis to cause anthrax have been acquired recently by horizontal gene transfer. to understand the genetic background for the evolution of b. anthracis virulence, we obtained high-redundancy genome sequences of 45 strains of the bacillus cereus sensu lato (s.l.) species that were chosen for their genetic diversity within the species based on the existing multilocus sequence typing scheme. from the resulting data, we called more than 324,000 new genes repres ...201222645259
preliminary study on the antimicrobial activity of enicostemma littorale using different solvents.to study the antimicrobial activity of enicostemma littorale (e. littorale) using different solvents.201222647818
genome characteristics of a novel phage from bacillus thuringiensis showing high similarity with phage from bacillus cereus.bacillus thuringiensis is an important entomopathogenic bacterium belongs to the bacillus cereus group, which also includes b. anthracis and b. cereus. several genomes of phages originating from this group had been sequenced, but no genome of siphoviridae phage from b. thuringiensis has been reported. we recently sequenced and analyzed the genome of a novel phage, btcs33, from a b. thuringiensis strain, subsp. kurstaki cs33, and compared the gneome of this phage to other phages of the b. cereus ...201222649540
efficient isolation and identification of bacillus cereus group.bacillus cereus is a group of ubiquitous facultative anaerobic sporeforming gram-positive rods commonly found in soil. the spores frequently contaminate a variety of foods, including produce, meat, eggs, and dairy products. foodborne illnesses associated with toxins produced by b. cereus can result in self-limiting diarrhea or vomiting. plate enumeration methods recommended by recognized food authorities to detect the presence of b. cereus in potentially contaminated food products do not inhibit ...201222649932
synthesis of a new class of antimicrobial agents incorporating the indolin-2-one moiety.new furanone derivatives incorporating the indolin-2-one moiety 3 were prepared via the perkin reaction of isatins 1 with aroylpropionic acids 2 under conventional conditions or microwave irradiation. a series of functionally heterocyclic derivatives (e.g., pyridazines, pyrroles, and sulfonamides) incorporating the indolin-2-one moiety was achieved via reaction of 3 with different reagents under microwave irradiation conditions. the newly synthesized compounds were characterized on the basis of ...201322651801
association of bacillus cereus infection with contaminated alcohol prep pads.bacillus species have caused healthcare-associated outbreaks of invasive disease as well as pseudo-outbreaks. we report an outbreak investigation of blood cultures positive for bacillus cereus associated with alcohol prep pads (apps) contaminated with b. cereus and bacillus species resulting in a rapid internal product recall and subsequent international product recall.201222669227
structure, biosynthesis, and properties of kurstakins, nonribosomal lipopeptides from bacillus spp.a new family of lipopeptides produced by bacillus thuringiensis, the kurstakins, was discovered in 2000 and considered as a biomarker of this species. kurstakins are lipoheptapeptides displaying antifungal activities against stachybotrys charatum. recently, the biosynthesis mechanism, the regulation of this biosynthesis and the potential new properties of kurstakins were described in the literature. in addition, kurstakins were also detected in other species belonging to bacillus genus such as b ...201222678024
synthesis and evaluation of antimicrobial activity of 4h-pyrimido[2,1-b]benzothiazole, pyrazole and benzylidene derivatives of curcumin.a novel, one-pot, simple, efficient procedure for 4h-pyrimido[2,1-b]benzothiazole (4a-h), pyrazole (6a-d) and benzylidene (7a-d) derivatives of curcumin under solvent and solvent free conditions in microwave with good yield is have been synthesized. the synthesized compounds were evaluated for their antibacterial activity against gram-positive and gram-negative bacteria viz. staphylococcus aureus, pseudomonas aeruginosa, salmonella typhi, escherichia coli, bacillus cereus and providencia rettger ...201222683240
generation of thiocillin ring size variants by prepeptide gene replacement and in vivo processing by bacillus cereus.the thiocillins from bacillus cereus atcc 14579 are natural products from the broader class of thiazolyl peptides. their biosynthesis proceeds via extensive post-translational modification of a ribosomally encoded precursor peptide. this post-translational tailoring involves a key step formal cycloaddition between two distal serine residues. in the wild-type structure, this cycloaddition forms a major macrocycle circumscribed by 26-atoms (shortest path). results presented herein demonstrate the ...201222686940
draft genome sequence of bacillus cereus strain lct-bc244.bacillus cereus is a prevalent, soil-dwelling, gram-positive bacterium. some strains are harmful to humans and cause food-borne illness, while other strains can be beneficial as probiotics for animals. to gain insight into the bacterial genetic determinants, we report the genome sequence of a strain, lct-bc244, which was isolated from cgmcc 1.230.201222689237
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