Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| lipopolysaccharides mediate leukotoxin secretion in aggregatibacter actinomycetemcomitans. | we previously reported that lipopolysaccharide (lps) -related sugars are associated with the glycosylation of the collagen adhesin emaa, a virulence determinant of aggregatibacter actinomycetemcomitans. in this study, the role of lps in the secretion of other virulence factors was investigated. the secretion of the epithelial adhesin aae, the immunoglobulin fc receptor omp34 and leukotoxin were examined in a mutant strain with inactivated tdp-4-keto-6-deoxy-d-glucose 3,5-epimerase (rmlc), which ... | 2011 | 22394466 |
| atomic force spectroscopy evidence of non-specific adhesion of aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans is a gram-negative periodontopathogen found within the subgingival plaque on the tooth surface. it is associated with localized aggressive periodontitis, a severe form of periodontitis in adolescents, and is the cause various extra-oral infections. the ability of this organism to tenaciously adhere to abiotic surfaces has been attributed to bundle-forming type ivb-like fimbriae whose major component is the fimbrial lower molecular weight protein (flp). in th ... | 2011 | 22400208 |
| comparative evaluation of the effects of different photoablative laser irradiation protocols on the gingiva of periodontopathic patients. | we aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode gaas, er:yag, nd:yag, and co(2) lasers) and histologically analyzing their effects on the gingiva. | 2012 | 22401128 |
| clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical periodontal treatment: a 6-month clinical trial. | this randomized split-mouth clinical trial was designed to evaluate the efficacy of scaling and root planing associated to the high-intensity diode laser on periodontal therapy by means of clinical parameters and microbial reduction. | 2013 | 22402617 |
| in vitro antibacterial efficacy of cetylpyridinium chloride-containing mouthwashes. | the objective of this study was to examine the ability of three cpc-containing mouthwashes to kill planktonic bacteria in an in vitro short-exposure assay. | 2011 | 22403973 |
| pdt in non-surgical treatment of periodontitis in hiv patients: a split-mouth, randomized clinical trial. | the aim of this study was to evaluate the clinical and microbiological effect of photodynamic therapy (pdt) in the non-surgical treatment of periodontitis in hiv patients. | 2012 | 22407618 |
| genome sequence of a serotype b non-jp2 aggregatibacter actinomycetemcomitans strain, anh9381, from a periodontally healthy individual. | gram-negative aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into jp2 and non-jp2 genotypes, with the former found to be more pathogenic than the latter. here we report the first complete genome sequence of a serotype b non-jp2 strain of a. actinomycetemcomitans. | 2012 | 22408240 |
| the green tea polyphenol (-)-epigallocatechin gallate precipitates salivary proteins including alpha-amylase: biochemical implications for oral health. | green tea is a popular drink throughout the world, and it contains various components, including the green tea polyphenol (-)-epigallocatechin gallate (egcg). tea interacts with saliva upon entering the mouth, so the interaction between saliva and egcg interested us, especially with respect to egcg-protein binding. sds-page revealed that several salivary proteins were precipitated after adding egcg to saliva. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ... | 2012 | 22409219 |
| distribution of periodontopathic bacterial species in dogs and their owners. | presently, a large number of individuals consider their companion animals as family members and have close contact with them in daily life. the purpose of the present study was to analyze the distribution of periodontopathic bacterial species in oral specimens taken from dogs and their owners. | 2012 | 22417880 |
| microbial profiles of patients seeking treatment for periodontitis. influence of origin, smoking and age? | we assessed the potential influence of the origin, the smoking status and the age on subgingival microbial profiles of subjects seeking periodontal care in switzerland today. | 2012 | 22419513 |
| increased levels of porphyromonas gingivalis are associated with ischemic and hemorrhagic cerebrovascular disease in humans: an in vivo study. | this study investigated the role of periodontal disease in the development of stroke or cerebral infarction in patients by evaluating the clinical periodontal conditions and the subgingival levels of periodontopathogens. | 2012 | 22437687 |
| individual composition of human leukocyte antigens and periodontopathogens in the background of periodontitis. | human leukocyte antigens (hlas) are a basic precondition to induce the immune response to pathogens. therefore, this study evaluates associations among periodontitis, five key periodontopathic bacteria, and hlas to test their impact together with additional risk factors in multivariate analyses. | 2013 | 22443520 |
| specificity of antimicrobial peptide ll-37 to neutralize periodontopathogenic lipopolysaccharide activity in human oral fibroblasts. | the antimicrobial peptide ll-37 is known to have a potent lipopolysaccharide (lps)-neutralizing activity in various cell types. because of observed heterogeneity within periodontopathogenic lps, the authors hypothesized that ll-37 had specificity to neutralize such lps activity. the present study, therefore, aims to investigate the lps-neutralizing activity of ll-37 to various periodontopathogenic lps in interleukin-8 (il-8) production after challenging them in human oral fibroblasts. | 2013 | 22443521 |
| aggregatibacter actinomycetemcomitans infection enhances apoptosis in vivo through a caspase-3-dependent mechanism in experimental periodontitis. | the purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by aggregatibacter actinomycetemcomitans infection. thirty-eight diabetic and 33 normal rats were inoculated with a. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. bone loss and the infiltration of polymorphonuclear leukocytes (pmns) in gingival epithelium were measured in hematoxylin-eosin-stained sections. the induction of tumor necrosis fa ... | 2012 | 22451521 |
| in vitro bactericidal activity against periodontopathic bacteria by electrolyzed ion-reduced water. | as typical periodontopathic bacteria, porphyromonas gingivalis (p. gingivalis) and aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) were exposed to electrolyzed ion-reduced water (eri) and eri containing 1% sodium carboxymethylcellulose (cmc-na) (eri-1% cmc-na), and the time course of their bactericidal action was evaluated. more than 99% of each bacteria species were killed after exposure to each solution for 15 sec. in addition, 1% cmc-na, which was added to prolong bactericida ... | 2011 | 22466442 |
| inhibitory activity of aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria. | aloe vera is a medicinal plant with anti-inflammatory, antimicrobial, antidiabetic and immune-boosting properties. in the present study we investigated the inhibitory activities of aloe vera gel on some cariogenic (streptococcus mutans), periodontopathic (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis) and an opportunistic periodontopathogen (bacteroides fragilis) isolated from patients with dental caries and periodontal diseases. twenty isolates of each of these bacteria were i ... | 2012 | 22466882 |
| leukotoxin (leukothera®) targets active leukocyte function antigen-1 (lfa-1) protein and triggers a lysosomal mediated cell death pathway. | leukotoxin (ltxa) is a protein toxin that is secreted from the oral bacterium, aggregatibacter actinomycetemcomitans. ltxa targets specifically the β(2) integrin, leukocyte function antigen-1 (lfa-1) on white blood cells (wbcs) and causes cell death. ltxa preferentially targets activated wbcs and is being developed as a therapeutic agent for the treatment of wbc diseases such as hematologic malignancies and autoimmune/inflammatory diseases. however, the mechanism by which interaction between ltx ... | 2012 | 22467872 |
| aggregatibacter actinomycetemcomitans serotypes, the jp2 clone and cytolethal distending toxin genes in a thai population. | to examine the genetic diversity of aggregatibacter actinomycetemcomitans in thai adults. | 2012 | 22471788 |
| aggregatibacter actinomycetemcomitans lipopolysaccharide regulates bone sialoprotein gene transcription. | aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) is believed to be associated with aggressive periodontitis characterized by a rapid bone loss. a. actinomycetemcomitans lipopolysaccharide (lps) has a similar structure to escherichia coli lps, and they are toll-like receptor 4 agonists. bone sialoprotein (bsp) is an early marker of osteoblast differentiation. to investigate the effects of a. actinomycetemcomitans lps on bone formation, we targeted bsp as a marker for osteogenic di ... | 2012 | 22492284 |
| production of a novel quercetin glycoside through metabolic engineering of escherichia coli. | most flavonoids exist as sugar conjugates. naturally occurring flavonoid sugar conjugates include glucose, galactose, glucuronide, rhamnose, xylose, and arabinose. these flavonoid glycosides have diverse physiological activities, depending on the type of sugar attached. to synthesize an unnatural flavonoid glycoside, actinobacillus actinomycetemcomitans gene tll (encoding dtdp-6-deoxy-l-lyxo-4-hexulose reductase, which converts the endogenous nucleotide sugar dtdp-4-dehydro-6-deoxy-l-mannose to ... | 2012 | 22492444 |
| aggregatibacter actinomycetemcomitans targets nlrp3 and nlrp6 inflammasome expression in human mononuclear leukocytes. | periodontitis is an inflammatory condition that destroys the tooth-supporting tissues, as a result of local bacterial infection. aggregatibacter actinomycetemcomitans is a gram-negative facultative anaerobic species, highly associated with aggressive periodontitis. periodontal inflammation is dominated by cytokines of the interleukin (il)-1 family. prior to their secretion by mononuclear cells, il-1 cytokines are processed by intracellular protein complexes, known as "inflammasomes", which can s ... | 2012 | 22503597 |
| bacterial colonization patterns of periodontal pockets in different ages. | the aim of this study was to investigate subgingival bacterial composition of untreated italian subjects with aggressive and chronic periodontitis. the total bacterial load, pathogenic bacteria belonging to "red" and "orange" complexes and aggregatibacter actinomycetemcomitans were determined by real-time pcr in 1216 patients. data were analysed by looking for relationships between bacteriological parameters, age and periodontal probing depth. the obtained results showed a significant higher num ... | 2013 | 22507992 |
| involvement of toll-like receptor 2 in apoptosis of aggregatibacter actinomycetemcomitans-infected thp-1 cells. | aggregatibacter (actinobacillus) actinomycetemcomitans is a gram-negative bacterium that has been associated with aggressive periodontitis. a actinomycetemcomitans infection induces apoptosis in the human monocytic cell line thp-1, and p38 mitogen-activated protein kinase (p38) activity and tumor necrosis factor-α (tnf-α) production are enhanced by a actinomycetemcomitans infection. however, mechanisms governing the recognition of a actinomycetemcomitans by monocytes during apoptosis have not be ... | 2013 | 22513358 |
| probiotics and periodontal health. | periodontitis is one of the most common chronic inflammatory diseases. the etiology is clearly bacterial and a number of putative bacterial pathogens have been associated with the disease, including aggregatibacter actinomycetemcomitans, tannerella forsythus and porphyromonas gingivalis. comparatively, little attention has been paid to the identification of health-associated and potentially beneficial bacterial species that may reside in the gingival sulcus. probiotic technology represents a bre ... | 2011 | 22514571 |
| mast cells act as phagocytes against the periodontopathogen aggregatibacter actinomycetemcomitans. | evidence to date shows that mast cells play a critical role in immune defenses against infectious agents, but there have been no reports about involvement of these cells in eliminating periodontopathogens. in this study, the phagocytic ability of mast cells against aggregatibacter actinomycetemcomitans compared with macrophages is evaluated. | 2013 | 22524328 |
| quantification of subgingival bacterial pathogens at different stages of periodontal diseases. | anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ... | 2012 | 22526568 |
| oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. | oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients. | 2012 | 22531876 |
| bacterial and inflammatory behavior of implants in the early healing phase of chronic periodontitis. | to assess the pattern of early bacterial colonization at implants and teeth in patients with a history of chronic periodontitis compared with a group of healthy subjects. furthermore, the presence of host-derived markers at teeth and implants in the two subject groups was determined. | 2012 | 22532956 |
| interactions of extracts from selected chewing stick sources with aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans produces a leukotoxin that activates a pro-inflammatory death of human monocytes/macrophages. a specific clone of this bacterium (jp2) has a 530-base pair deletion in the leukotoxin promoter gene and significantly enhanced expression of leukotoxin. this specific clone of a. actinomycetemcomitans is common in some african populations and has a strong association with periodontal attachment loss in adolescents in these populations. chewing sticks of plant orig ... | 2012 | 22537711 |
| serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease. | chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects. | 2012 | 22546352 |
| prevalence of fima genotypes of porphyromonas gingivalis and other periodontal bacteria in a spanish population with chronic periodontitis. | the aim of this study was to determine the prevalence of the different fima genotypes of porphyromonas gingivalis in adult spanish patients with chronic periodontitis, patients with gingivitis and periodontally healthy subjects, and the relationship between these genotypes and other periodontopathogenic bacteria. | 2012 | 22549664 |
| subgingival microbial profiles as diagnostic markers of destructive periodontal diseases: a clinical epidemiology study. | to describe the subgingival microbial profiles of the major putative periodontal pathogens and investigate their role as diagnostic markers for destructive periodontal diseases in an untreated and isolated population. | 2013 | 22564019 |
| effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. | emingil g, han b, özdemir g, tervahartiala t, vural c, atilla g, baylas h, sorsa t. the effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. j periodont res 2012; 47: 729-739. © 2012 john wiley & sons a/s background and objective: to study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parame ... | 2012 | 22571226 |
| experimental arthritis exacerbates aggregatibacter actinomycetemcomitans-induced periodontitis in mice. | this study aimed to investigate whether chronic antigen-induced arthritis (aia) influences infection-induced periodontitis (pd) in mice and whether pd modifies the clinical course of aia. the contribution of anti-tnf-α therapy was also evaluated. | 2012 | 22582749 |
| human salivary cystatin sa exhibits antimicrobial effect against aggregatibacter actinomycetemcomitans. | healthy subjects who do not have aggregatibacter actinomycetemcomitans in their oral cavity may possess factors in saliva that might demonstrate antibacterial activity against the bacterium. the aim of this study was to identify and purify proteins from saliva of healthy subjects that might demonstrate antibacterial activity against a. actinomycetemcomitans and test the same against the bacteria. | 2012 | 22582873 |
| fcγriib-nt645+25a/g gene polymorphism and periodontitis in japanese women with preeclampsia. | fcγriib contains a unique immunoreceptor tyrosine-based inhibition motif (itim) and functions as a negative feedback regulator of leucocyte activation and antibody production. we have previously reported fcγriib-nt645+25a/g gene polymorphism to be associated with prevalence and severity of periodontitis, fcγriib expression level on peripheral b lymphocytes and the serum igg level against periodontopathic bacteria. previous studies have reported maternal periodontal disease to be associated with ... | 2012 | 22594540 |
| relationship between gram negative enteric rods, aggregatibacter actinomycetemcomitans, and clinical parameters in periodontal disease. | the association between gram negative enteric rods and aggregatibacter actinomycetemcomitans in periodontal diseases has received little attention in the literature. the objective of this study was to explore the relationship between these organisms and clinical parameters of periodontal disease. | 2012 | 22628966 |
| localization of aggregatibacter actinomycetemcomitans cytolethal distending toxin subunits during intoxication of live cells. | the cytolethal distending toxin (cdt), produced by some clinically important gram-negative bacterial species, is related to the family of ab-type toxins. three heterologous proteins (cdta, cdtb, and cdtc) and a genotoxin mode of action distinguish the cdt from others in this toxin class. crystal structures of several species-specific cdts have provided a basis for predicting subunit interactions and functions. in addition, empirical studies have yielded significant insights into the in vivo inte ... | 2012 | 22645284 |
| peripheral arterial disease associated with caries and periodontal disease. | peripheral arterial disease (pad) is an important cardiovascular disorder of the peripheral arteries. chronic infections, such as periodontitis, may play an important role in the etiology and pathophysiology of pad and other cardiovascular conditions. recently, streptococcus mutans has been found with high frequency in atheromatous plaques. the aim of this study is to evaluate the possible clinical and microbiologic association between pad and periodontitis and dental caries. | 2013 | 22680302 |
| o-polysaccharide glycosylation is required for stability and function of the collagen adhesin emaa of aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans is hypothesized to colonize through the interaction with collagen and establish a reservoir for further dissemination. the trimeric adhesin emaa of a. actinomycetemcomitans binds to collagen and is modified with sugars mediated by an o-antigen polysaccharide ligase (waal) that is associated with lipopolysaccharide (lps) biosynthesis (g. tang and k. mintz, j. bacteriol. 192:1395-1404, 2010). this investigation characterized the function and cellular localizat ... | 2012 | 22689812 |
| antimicrobial constituents of artemisia afra jacq. ex willd. against periodontal pathogens. | the phytochemical investigation of an ethanol extract of artemisia afra led to the isolation of six known compounds, acacetin (1), 12α,4α-dihydroxybishopsolicepolide (2), scopoletin (3), α-amyrin (4), phytol (5), and a pentacyclic triterpenoid betulinic acid (6). the compounds were evaluated for antimicrobial activity against gram positive (actinomyces naeslundii, actinomyces israelii, and streptococcus mutans), gram negative bacteria (prevotella intermedia, porphyromonas gingivalis and aggregat ... | 2012 | 22693528 |
| genome-wide association study of periodontal pathogen colonization. | pathological shifts of the human microbiome are characteristic of many diseases, including chronic periodontitis. to date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. we conducted a genome-wide association (gwa) study among 1,020 white participants of the atherosclerosis risk in communities study, whose periodontal diagnosis ranged from healthy to severe chronic periodontitis, and for whom "checkerboard" dna-dna hybridization quantificat ... | 2012 | 22699663 |
| a common periodontal pathogen has an adverse association with both acute and stable coronary artery disease. | the aim of this study was to investigate the association between angiographically verified coronary artery disease (cad) and salivary levels of four major periodontal pathogens. | 2012 | 22704805 |
| exogenous administration of 15d-pgj2-loaded nanocapsules inhibits bone resorption in a mouse periodontitis model. | the 15-deoxy-(δ12,14)-pg j(2) (15d-pgj(2)) has demonstrated excellent anti-inflammatory results in different experimental models. it can be used with a polymeric nanostructure system for modified drug release, which can change the therapeutic properties of the active principle, leading to increased stability and slower/prolonged release. the aim of the current study was to test a nanotechnological formulation as a carrier for 15d-pgj(2), and to investigate the immunomodulatory effects of this fo ... | 2012 | 22706081 |
| subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions. | this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ... | 2012 | 22710412 |
| [amalgam, composite and compomer: microbiological study]. | restorative materials have different consequences on the periodontium. the surface of these materials may influence gingival health and cause in some instances gingival inflammation. the purpose of this study was to investigate and compare, in a healthy periodontium, intracrevicular plaque bacteria (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis and treponema denticola), at day 0 and at 6 months, around subgingivally located amalga ... | 2012 | 22715643 |
| haemophilus aphrophilus associated spleen abscess: an unusual presentation of subacute endocarditis. | the hacek group of bacteria (haemophilus spp., actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, kingella spp.), is uncommon pathogens of infective endocarditis, but can cause life-threatening events such as heart failure or formation of lethal emboli. here we report a 58-year-old asian man with a past history of congenital valvular heart disease who presented with sudden onset of left flank pain followed by fever with chills for 2 weeks. abdominal computed tomo ... | 2012 | 22719808 |
| antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens. | dental plaque accumulation may result in peri-implantitis, an inflammatory process causing loss of supporting bone that may lead to dental implant failure. the antimicrobial activities of six metal and metal oxide nanoparticles and two of their composites against bacterial pathogens associated with peri-implantitis were examined under anaerobic conditions. the activities of nanoparticles of silver (ag), cuprous oxide (cu(2)o), cupric oxide (cuo), zinc oxide (zno), titanium dioxide (tio(2)), tung ... | 2012 | 22727529 |
| clinical and microbiological parameters in patients with self-ligating and conventional brackets during early phase of orthodontic treatment. | to determine the effect of different bracket designs (conventional brackets and self-ligating brackets) on periodontal clinical parameters and periodontal pathogens in subgingival plaque. | 2013 | 22765511 |
| actinobacillus actinomycetemcomitans endocarditis in a 1.5 year old toddler. | the authors report a 1.5-year-old girl who developed actinobacillus actinomycetemcomitans (aa) endocarditis involving the pulmonic valve. she had a congenital cardiac abnormality, but no history of dental manipulation. the case illustrates an uncomplicated course with three unique features; the youngest reported infant with endocarditis caused by aa with vegetation on the pulmonic valve. she underwent a benign course with complete recovery. the authors report a 1.5-year-old girl who developed aa ... | 2010 | 22767569 |
| serotype-dependent expression patterns of stabilized lipopolysaccharide aggregates in aggregatibacter actinomycetemcomitans strains. | above a critical concentration, amphiphilic lipopolysaccharide (lps) molecules in an aqueous environment form aggregate structures, probably because of interactions involving hydrophobic bonds. ionic bonds involving divalent cations stabilize these aggregate structures, making them resistant to breakdown by detergents. the aim of this study was to examine expression patterns of stabilized lps aggregates in aggregatibacter actinomycetemcomitans, a microorganism that causes periodontitis. a. actin ... | 2012 | 22812537 |
| an in vitro investigation concerning the bacterial leakage at implants with internal hexagon and morse taper implant-abutment connections. | to evaluate, in vitro, the leakage observed in internal hexagon and morse taper implant-abutment connections. | 2012 | 22814560 |
| [fungal invasion of connective tissue in patients with gingival-periodontal disease]. | in the last few years unusual microorganisms have been isolated from subgingival biofilm, as possible initiators or contributors to periodontal disease, especially in patients who show no improvement during treatment. | 2015 | 22824245 |
| proteomics of protein secretion by aggregatibacter actinomycetemcomitans. | the extracellular proteome (secretome) of periodontitis-associated bacteria may constitute a major link between periodontitis and systemic diseases. to obtain an overview of the virulence potential of aggregatibacter actinomycetemcomitans, an oral and systemic human pathogen implicated in aggressive periodontitis, we used a combined lc-ms/ms and bioinformatics approach to characterize the secretome and protein secretion pathways of the rough-colony serotype a strain d7s. lc-ms/ms revealed 179 pr ... | 2012 | 22848560 |
| antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects. | neutrophils play an important role in periodontitis by producing nitric oxide (no) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. it is unknown whether variation in the production of antimicrobial peptides such as ll-37, human neutrophil peptides (hnp) 1-3, and no by neutrophils influences the pathogenesis of periodontal diseases. we compared the production of these peptides and no by lipopolysaccharide (lps)-stimu ... | 2012 | 22850872 |
| microbiological characterization in children with aggressive periodontitis. | the objective of this study was to characterize the subgingival microbiota of african-american children with localized aggressive periodontitis (lap). fifty-one children were included. subgingival plaque samples were taken from diseased (dd) and healthy sites (dh) in lap and from healthy sites in hs and hc and analyzed by 16s rrna-based microarrays. aggregatibacter actinomycetemcomitans (aa) was the only species found to be both more prevalent (or = 8.3, p = 0.0025) and abundant (p < 0.01) in dd ... | 2012 | 22863892 |
| are there specific benefits of amoxicillin plus metronidazole in aggregatibacter actinomycetemcomitans-associated periodontitis? double-masked, randomized clinical trial of efficacy and safety. | it has been suggested that prescription of amoxicillin plus metronidazole in the context of periodontal therapy should be limited to patients with specific microbiologic profiles, especially those testing positive for aggregatibacter actinomycetemcomitans. the main purpose of this analysis is to determine if patients positive for a. actinomycetemcomitans with moderate to advanced periodontitis benefit specifically from amoxicillin plus metronidazole given as an adjunct to full-mouth scaling and ... | 2013 | 22873656 |
| prediagnostic plasma antibody levels to periodontopathic bacteria and risk of coronary heart disease. | many epidemiological studies have indicated that periodontitis is an important risk factor for coronary heart disease (chd). we examined whether plasma antibody levels to 3 major periodontal pathogens, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia predicted the risk of chd events. a nested case-control research design (case: n = 191, control: n = 382), by matching gender, age, study area, date of blood collection, and time since last meal at blood col ... | 2012 | 22878796 |
| the periodontal pathogen aggregatibacter actinomycetemcomitans deteriorates ventricular remodeling after myocardial infarction in mice. | chronic inflammation plays a fundamental role in coronary heart disease (chd). periodontal disease is a common infectious disease and is a potential source of systemic inflammation. however, the effect of periodontal infection on chd has not yet been proven. the purpose of this study was to determine the effect of periodontopathic bacteria on experimental myocardial infarction (mi). we implanted a chamber into the subcutaneous tissue of each male mouse. aggregatibacter actinomycetemcomitans (a.a ... | 2012 | 22878805 |
| periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease. | to determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hhsp60) antibodies in patients with established cardiovascular disease (cvd). | 2012 | 22882677 |
| neutralizing effect of green tea epigallocatechin-3-gallate on nicotine-induced toxicity and chemokine (c-c motif) ligand 5 secretion in human oral epithelial cells and fibroblasts. | tobacco use has been identified as the most important environmental risk factor for periodontitis. the aim of this study was to investigate the effect of green tea epigallocatechin-3-gallate on the nicotine-induced toxic and inflammatory responses in oral epithelial cells and gingival fibroblasts. | 2011 | 22887905 |
| antibiotic susceptibility profiles of oral pathogens. | periodontitis is a bacterial disease that can be treated with systemic antibiotics. the aim of this study was to establish the antibiotic susceptibility profiles of five periodontal pathogens to six commonly used antibiotics in periodontics. a total of 247 periodontal bacterial isolates were tested for susceptibility to the six antibiotics using the etest method. mic(50) and mic(90) values (minimum inhibitory concentrations for 50% and 90% of the organisms, respectively) were calculated. both eu ... | 2012 | 22890195 |
| actinobacillus endocarditis associated with hypertrophic cardiomyopathy. | infective endocarditis can be associated with complex clinical presentations, sometimes with a difficult multi-disciplinary management. actinobacillus actinomycetemcomitans belongs to the haemophilus species, actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens and kingella species group, responsible for 5% to 10% of infective endocarditis in native heart valves. these organisms have slow fastidious growth pattern, often associated with negative cultures, and cause systemic emboli ... | 2012 | 22891010 |
| bacterial antagonism against periodontopathogens. | the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria. | 2013 | 22897652 |
| interleukin-1β is internalised by viable aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids. | the opportunistic pathogen aggregatibacter actinomycetemcomitans causes periodontitis, which is a biofilm infection that destroys tooth-supportive tissues. interleukin (il)-1β, a central proinflammatory cytokine of periodontitis, is an essential first line cytokine for local inflammation that modulates the cell proliferation and anti-pathogen response of human gingival keratinocytes. previously, we demonstrated that a. actinomycetemcomitans biofilms bind il-1β; however, whether this binding is a ... | 2012 | 22898394 |
| the peri-implant health in patients attending an annual recall program. a clinical and microbiological study in 74 patients from the tübingen implant registry. | to describe the peri-implant condition of unselected implant recall patients, to relate it to relevant features from the patient medical history, and to look at associations across these findings. | 2013 | 22905716 |
| leukotoxin from aggregatibacter actinomycetemcomitans causes shrinkage and p2x receptor-dependent lysis of human erythrocytes. | leukotoxin (ltxa) is a virulence factor secreted by the bacterium aggregatibacter actinomycetemcomitans, which can cause localized aggressive periodontitis and endocarditis. ltxa belongs to the repeat-in-toxin (rtx) family of exotoxins of which other members inflict lysis by formation of membrane pores. recently, we documented that the haemolytic process induced by another rtx toxin [α-haemolysin (hlya) from escherichia coli] requires p2x receptor activation and consists of sequential cell shrin ... | 2012 | 22906303 |
| influence of il-6 haplotypes on clinical and inflammatory response in aggressive periodontitis. | the aim of this study was to investigate the inflammatory response in aggressive periodontitis (agp) patients after periodontal therapy and associate these changes to subjects' interleukin-6 (il-6) genetic variants. | 2013 | 22918663 |
| analysis of viable vs. dead aggregatibacter actinomycetemcomitans and porphyromonas gingivalis using selective quantitative real-time pcr with propidium monoazide. | one of the major disadvantages of dna-based microbial diagnostics is their inability to differentiate dna between viable and dead microorganisms, which could be important when studying etiologically relevant pathogens. the aim of this investigation was to optimize a method for the selective detection and quantification of only viable aggregatibacter actinomycetemcomitans and porphyromonas gingivalis cells by combining quantitative real-time polymerase chain reaction (qpcr) and propidium monoazid ... | 2013 | 22957816 |
| novel iron-regulated and fur-regulated small regulatory rnas in aggregatibacter actinomycetemcomitans. | iron can regulate biofilm formation via non-coding small rna (srna). to determine if iron-regulated srnas are involved in biofilm formation by the periodontopathogen aggregatibacter actinomycetemcomitans, total rna was isolated from bacteria cultured with iron supplementation or chelation. transcriptional analysis demonstrated that the expression of four srna molecules (ja01-ja04) identified by bioinformatics was significantly upregulated in iron-limited medium compared with iron-rich medium. a ... | 2012 | 22958383 |
| characterization of the secretion pathway of the collagen adhesin emaa of aggregatibacter actinomycetemcomitans. | the extracellular matrix protein adhesin a (emaa) surface antennae-like structures of the periodontal pathogen aggregatibacter actinomycetemcomitans are composed of three identical protein monomers. recently, we have demonstrated that the protein is synthesized with an extended signal peptide of 56 amino acids necessary for membrane targeting and protein translocation. in this study, emaa secretion was demonstrated to be reliant on a chaperone-dependent secretion pathway. deletion of secb partia ... | 2012 | 22958387 |
| sustained mitogen-activated protein kinase activation with aggregatibacter actinomycetemcomitans causes inflammatory bone loss. | aggregatibacter actinomycetemcomitans is a gram-negative facultative capnophile involved in pathogenesis of aggressive forms of periodontal disease. in the present study, we interrogated the ability of a. actinomycetemcomitans to stimulate innate immune signaling and cytokine production and established that a. actinomycetemcomitans causes bone loss in a novel rat calvarial model. in vitro studies indicated that a. actinomycetemcomitans stimulated considerable production of soluble cytokines, tum ... | 2012 | 22958388 |
| do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens? | the presence of periodontal pathogens in the oral cavity may impact implant survival. therefore, this study aimed to determine the prevalence of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, prevotella intermedia, tannerella forsythia, treponema denticola, eikenella corrodens, and parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures. | 2012 | 22988660 |
| quantification of periodontopathic bacteria in saliva using the invader assay. | when quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. the invader assay is a convenient method because it does not involve gene amplification. the purpose of this study was to evaluate the validity of the invader assay to quantify periodontopathic bacteria. the invader technology was applied in quantifying five periodontopathic bacteria (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsy ... | 2012 | 22996215 |
| a double-blind randomized placebo-controlled study on the clinical and microbial effects of an essential oil mouth rinse used by patients in supportive periodontal care. | this 3-month double-blind randomized placebo-controlled study evaluated the clinical and microbial effects of an essential oil mouth rinse used as an adjunct to mechanical plaque control by patients in supportive periodontal care. | 2013 | 22998386 |
| molecular detection of aggregatibacter actinomycetemcomitans on metallic brackets by the checkerboard dna-dna hybridization technique. | the purpose of this randomized clinical study was to evaluate the presence of the periodontal pathogen aggregatibacter actinomycetemcomitans on metallic brackets and the effectiveness of a 0.12% chlorhexidine digluconate mouthwash in inhibiting this microorganism. | 2012 | 22999671 |
| detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women. | the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ... | 2012 | 23018230 |
| aggregatibacter actinomycetemcomitans in down's syndrome children. | to investigate the presence of aggregatibacter actinomycetemcomitans (aa) in down's syndrome (ds) children in comparison with age-matched normal children and to determine if aa was acquired during childhood in ds individuals. | 2012 | 23019843 |
| prophage induction in lysogenic aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release. | lysogeny is common among strains of the periodontal pathogen aggregatibacter actinomycetemcomitans. since lysogenic induction is known to result in the increased synthesis and release of bacterial toxins from lysogens, it would be important to elucidate the conditions under which induction of these bacteria may occur. co-cultures of a. actinomycetemcomitans strains (either lysogenic or non-lysogenic) and human cells (either gingival fibroblasts or pharyngeal epithelial cells) were prepared. foll ... | 2013 | 23022667 |
| an evolutionary link between natural transformation and crispr adaptive immunity. | natural transformation by competent bacteria is a primary means of horizontal gene transfer; however, evidence that competence drives bacterial diversity and evolution has remained elusive. to test this theory, we used a retrospective comparative genomic approach to analyze the evolutionary history of aggregatibacter actinomycetemcomitans, a bacterial species with both competent and noncompetent sister strains. through comparative genomic analyses, we reveal that competence is evolutionarily lin ... | 2012 | 23033473 |
| porphyromonas gingivalis displays a competitive advantage over aggregatibacter actinomycetemcomitans in co-cultured biofilm. | biofilm formation occurs through the events of cooperative growth and competitive survival among multiple species. porphyromonas gingivalis and aggregatibacter actinomycetemcomitans are important periodontal pathogens. the aim of this study was to demonstrate competitive or cooperative interactions between these two species in co-cultured biofilm. | 2013 | 23033940 |
| variables of host response in saliva of patients with periodontitis: a pilot study. | periodontitis is an infection that results from an imbalance between periodontopathic microorganisms and the local and systemic host defense. this study analyzed saliva samples of patients with periodontitis for several biomarkers of host response. | 2012 | 23034426 |
| periodontal pathogen aggregatibacter actinomycetemcomitans deteriorates pressure overload-induced myocardial hypertrophy in mice. | although a relationship between periodontitis and myocardial hypertrophy has been reported, the precise mechanism has not been clarified. the purpose of this study was to investigate the association between periodontal infection and myocardial hypertrophy. transverse aortic constriction (tac) was performed. mice were injected with aggregatibacter actinomycetemcomitans (a.a.) (0.1 ml of 10(8) cfu/ml) in the infected group and pbs in the control group. echocardiography, histopathology, and immunoh ... | 2012 | 23038095 |
| reduction of salivary arginine catabolic activity through periodontal therapy. | salivary enzymes may be used to diagnose periodontal conditions. salivary arginase activity (saa) is related to susceptibility to bacterial infection. therefore, the aim of this controlled interventional study was to determine the saa before and after non-surgical periodontal therapy. | 2012 | 23041992 |
| antibacterial activity of myristica fragrans against oral pathogens. | myristica fragrans houtt is mostly cultivated for spices in penang island, malaysia. the ethyl acetate and ethanol extracts of flesh, mace and seed of myristica fragrans was evaluated the bactericidal potential against three gram-positive cariogenic bacteria (streptococcus mutans atcc 25175, streptococcus mitis atcc 6249, and streptococcus salivarius atcc 13419) and three gram-negative periodontopathic bacteria (aggregatibacter actinomycetemcomitans atcc 29522, porphyromonas gingivalis atcc 3327 ... | 2012 | 23049613 |
| quantitative discrimination of aggregatibacter actinomycetemcomitans highly leukotoxic jp2 clone from non-jp2 clones in diagnosis of aggressive periodontitis. | aggregatibacter actinomycetemcomitans is the etiological agent of periodontitis, and there is a strong association between clone jp2 and aggressive periodontitis in adolescents of african descent. the jp2 clone has an approximately 530-bp deletion (∆530) in the promoter region of the lkt/ltx gene, which encodes leukotoxin, and this clone has high leukotoxic activity. therefore, this clone is very important in aggressive periodontitis. to diagnose this disease, culture methods and conventional pc ... | 2012 | 23050598 |
| clinical and microbiological changes after minimally invasive therapeutic approaches in intrabony defects: a 12-month follow-up. | this 12-month randomized, controlled trial evaluated the clinical effects and microbiological changes of minimally invasive nonsurgical and surgical approaches for the therapy of intrabony defects. | 2013 | 23053707 |
| effects of biphenyl sulfonylamino methyl bisphosphonic acids on porphyromonas gingivalis and cytokine secretion by oral epithelial cells. | bisphosphonate drugs are well known to inhibit osteoclastic activity and have been proposed for the management of bone diseases, including periodontitis which is associated with alveolar bone destruction. in this study, we evaluated the effects of four arylsulfonamide bisphosphonates on growth of the periodontopathogenic bacterium porphyromonas gingivalis as well as their capacity to reduce cytokine secretion by lipopolysaccharide (lps)-stimulated oral epithelial cells. the growth of p. gingival ... | 2013 | 23061603 |
| modeling susceptibility to periodontitis. | chronic inflammatory diseases like periodontitis have a complex pathogenesis and a multifactorial etiology, involving complex interactions between multiple genetic loci and infectious agents. we aimed to investigate the influence of genetic polymorphisms and bacteria on chronic periodontitis risk. we determined the prevalence of 12 single-nucleotide polymorphisms (snps) in immune response candidate genes and 7 bacterial species of potential relevance to periodontitis etiology, in chronic periodo ... | 2013 | 23100272 |
| transcriptional regulation of aggregatibacter actinomycetemcomitans lsracdbfg and lsrrk operons and their role in biofilm formation. | autoinducer-2 (ai-2) is required for biofilm formation and virulence of the oral pathogen aggregatibacter actinomycetemcomitans, and we previously showed that lsrb codes for a receptor for ai-2. the lsrb gene is expressed as part of the lsracdbfg operon, which is divergently transcribed from an adjacent lsrrk operon. in escherichia coli, lsrrk encodes a repressor and ai-2 kinase that function to regulate lsracdbfg. to determine if lsrrk controls lsracdbfg expression and influences biofilm growth ... | 2013 | 23104800 |
| cleavage of igg1 in gingival crevicular fluid is associated with the presence of porphyromonas gingivalis. | immunoglobulin (ig) g1 plays an important role in the adaptive immune response. kgp, a lysine-specific cysteine protease from porphyromonas gingivalis, specifically hydrolyses igg1 heavy chains. the purpose of this study was to examine whether cleavage of igg1 occurs in gingival crevicular fluid (gcf) in vivo, and whether there is any association with the presence of porphyromonas gingivalis and other periodontopathogens. | 2012 | 23116446 |
| possible translocation of periodontal pathogens into the lymph nodes draining the oral cavity. | numerous publications have reported the presence of periodontopathogenic bacteria in peripheral and central vascular lesions. however, it is unclear how this bacterial translocation occurs. the objective of this study was to investigate whether periodontopathic bacteria are translocated to lymph nodes proximal to the oral cavity. obtaining lymph node samples is not ethically feasible unless they are excised as part of the surgical management of patients with cancer. this study analyzed formalin- ... | 2012 | 23124752 |
| frequency of periodontal pathogens in equivalent peri-implant and periodontal clinical statuses. | this study tested the hypotheses that there is: (1) higher bacterial frequency in peri-implantitis/periodontitis, followed by mucositis/gingivitis and peri-implant/periodontal health; (2) similar bacterial frequency between comparable peri-implant and periodontal clinical statuses. | 2013 | 23127822 |
| gram-positive bacteria as an antigen topically applied into gingival sulcus of immunized rat accelerates periodontal destruction. | periodontitis is generally accepted to relate to gram-negative bacteria, and the host defense system influences its onset and progression. however, little is known about the relation between gram-positive bacteria and periodontitis. in this study, we topically applied gram-positive and gram-negative bacterial suspensions to the gingival sulcus in rats after immunization, and then histopathologically examined their influence on periodontal destruction. | 2013 | 23137272 |
| fenretinide inhibited de novo ceramide synthesis and proinflammatory cytokines induced by aggregatibacter actinomycetemcomitans. | ceramides play an essential role in modulating immune signaling pathways and proinflammatory cytokine production in response to infectious pathogens, stress stimuli, or chemotherapeutic drugs. in this study, we demonstrated that aggregatibacter actinomycetemcomitans, the pathogen for aggressive periodontitis, induced de novo synthesis of ceramide in raw 264.7 cells. in addition, we identified that fenretinide, a synthetic retinoid, suppressed the de novo synthesis of ceramide induced by a. actin ... | 2013 | 23139430 |
| screening for periodontopathogenic bacteria in severe chronic periodontitis in a moroccan population. | bacteria play an important role in destructive periodontitis. the aim of this study was to screen for five highly pathogenic bacteria: aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, and treponema denticola, in moroccan patients presenting with severe chronic periodontitis and to compare results with those of patients presenting with severe aggressive periodontitis. | 2012 | 23141872 |
| p2x receptor stimulation amplifies complement-induced haemolysis. | activation of the complement system evokes cell damage by insertion of membrane attack complexes, which constitute the basis of the pathogenesis of various haemolytic disorders. recently, we found that haemolysis caused by other types of membrane pore-forming proteins such as α-haemolysin (hlya) from escherichia coli, α-toxin from staphylococcus aureus and leukotoxin from aggregatibacter actinomycetemcomitans inflict their cytotoxic effects through p2 receptor activation. here we show that simil ... | 2013 | 23149487 |
| aggregatibacter actinomycetemcomitans groel protein promotes conversion of human cd4+ t cells into ifnγ il10 producing tbet+ th1 cells. | one of the heat shock family protein (hsp) expressing bacteria is the gram negative, periodontal pathogen aggregatibacter actinomycetemcomitans (aa). a. actinomycetemcomitans' hsp is a 64-kda groel-protein, which has been shown to influence the host cells. in this study we used recombinant a. actinomycetemcomitans groel (raagroel) protein as a model antigen to study groel-mediated t cell immune response. human peripheral mononuclear cells (pbmcs), when stimulated with recombinant raagroel, expre ... | 2012 | 23152883 |
| evaluation of efficacy of tetracycline fibers in conjunction with scaling and root planing in patients with chronic periodontitis. | the objective of this study was to compare the efficacy of scaling and root planing (srp) alone versus tetracycline fiber therapy used adjunctively with srp in the treatment of chronic periodontitis sites in maintenance patients. | 2012 | 23162335 |
| protective effect of topical cordia verbenacea in a rat periodontitis model: immune-inflammatory, antibacterial and morphometric assays. | this study evaluated the effects of c. verbenacea essential oil topically administered in a rat periodontitis model. | 2012 | 23171319 |