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distinct roles of comk1 and comk2 in gene regulation in bacillus cereus.the b. subtilis transcriptional factor comk regulates a set of genes coding for dna uptake from the environment and for its integration into the genome. in previous work we showed that bacillus cereus expressing the b. subtilis comk protein is able to take up dna and integrate it into its own genome. to extend our knowledge on the effect of b. subtilis comk overexpression in b. cereus we first determined which genes are significantly altered. transcriptome analysis showed that only part of the c ...201121747963
bioinformatics and molecular approaches to detect nrps genes involved in the biosynthesis of kurstakin from bacillus thuringiensis.degenerated primers designed for the detection by polymerase chain reaction of nonribosomal peptide synthetases (nrps) genes involved in the biosynthesis of lipopeptides were used on genomic dna from a new isolate of bacillus thuringiensis cip 110220. primers dedicated to surfactin and bacillomycin detection amplified sequences corresponding respectively to the surfactin synthetase operon and to a gene belonging to a new nrps operon identified in the genome of b. thuringiensis serovar pondicheri ...201121751008
mukbef on the march: taking over chromosome organization in bacteria?smc-scpab complexes, also called bacterial condensin, are comprised of highly conserved proteins present in a wide variety of bacteria and archaea. they facilitate bacterial chromosome segregation probably by organizing and/or compacting nascent sister chromosomes. in some ?-proteobacteria however the smc-scpab complex appears to have been replaced by a different smc complex, called mukbef. petrushenko et al., (2011) now show in this issue of molecular microbiology that distantly related homolog ...201121752108
antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate schiff base ligands and their metal complexes.in this study, two schiff base ligands (hl(1) and hl(2)) and their cu(ii), co(ii), ni(ii), pd(ii) and ru(iii) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. the ligands and their metal complexes were evaluated for their antimicrobial activity against corynebacterium xerosis, bacillus brevis, bacillus megaterium, bacillus cereus, mycobacterium smegmatis, st ...201121752697
evaluation of four microbial class ii fructose 1,6-bisphosphate aldolase enzymes for use as biocatalysts.fructose 1,6-bisphosphate (fbp) aldolase has been used as biocatalyst in the synthesis of several pharmaceutical compounds such as monosaccharides and analogs. is has been suggested that microbial metal-dependant class ii aldolases could be better industrial catalysts than mammalian class i enzyme because of their greater stability. the class ii aldolases from four microbes were subcloned into the escherichia coli vector pt7-7, expressed and purified to near homogeneity. the kinetic parameters, ...201121763425
l-asparaginase production by mangrove derived bacillus cereus mab5: optimization by response surface methodology.to isolate marine bacteria, statistically optimize them for maximum asparaginase production.201121771705
antioxidative and antibacterial effects of seeds and fruit rind of nutraceutical plants belonging to the fabaceae family.in the present study, the seeds and fruit rind of six plants of the fabaceae family were selected to evaluate their potential as antioxidant and antibacterial agents. the dried powders were individually extracted with various organic solvents by the cold percolation method, were evaluated for antibacterial activity and methanol extracts used for antioxidant activities. total phenol, protein and sugar contents were also measured. antioxidant activities were measured by dpph free radical scavengin ...201021776481
the analysis of the immobilization mechanism of ni(ii) on bacillus cereus.this work focused on the identification of biosorption mechanism of ni(ii) by living bacillus cereus (b. cereus) based on batch experiments and a variety of microscopic equipments. the adsorption equilibrium reached rapidly in 2 h and the maximum nickel adsorption capability of b. cereus was 17.7 mg x g(-1) (dry weight). atomic force microscopy (afm) analysis showed that the bacterial surface roughness increased from 7.9 +/- 0.5 nm to 12.6 +/- 1.6 nm during this process. scanning electron micros ...201121776742
domains of bcla, the major surface glycoprotein of the b. cereus exosporium: glycosylation patterns and role in spore surface properties.the role of the bcla domains of b. cereus atcc 14579 was investigated in order to understand the phenomena involved in the interfacial processes occurring between spores and inert surfaces. this was done by (i) creating deletions in the collagen-like region (clr) and the c-terminal domain (ctd) of bcla, (ii) building bcla proteins with various lengths in the clr and (iii) modifying the hydrophobic upper surface in the ctd. first, it was demonstrated that the clr was substituted by three residues ...201121777114
narrow antagonistic activity of antimicrobial peptide from bacillus subtilis sck-2 against bacillus cereus.bacillus subtilis sck-2, producing an antimicrobial peptide of this study, was isolated from kyeopjang, the korean traditional fermented-soybean paste. this strain showed a narrow antagonistic activity as it inhibited bacillus cereus causing food poisoning in human. the antimicrobial peptide, tentatively named amp ic-1, was purified, characterized, and compared to bsap-254, another peptide which was previously recovered from traditionally fermented-soybean paste. amp ic-1 was found to be more th ...201121783410
environmental and biofilm-dependent changes in a bacillus cereus secondary cell wall polysaccharide.bacterial species from the bacillus genus, including bacillus cereus and bacillus anthracis, synthesize secondary cell wall polymers (scwp) covalently associated to the peptidoglycan through a phospho-diester linkage. although such components were observed in a wide panel of b. cereus and b. anthracis strains, the effect of culture conditions or of bacterial growth state on their synthesis has never been addressed. herein we show that b. cereus atcc 14579 can synthesize not only one, as previous ...201121784857
purification and characterization of an endoxylanase from the culture broth of bacillus cereus bsa1.an extracellular xylanase from the fermented broth of bacillus cereus bsa1 was purified and characterized. the enzyme was purified to 3.43 fold through ammonium sulphate precipitation, deae-cellulose chromatography and followed by gel filtration through sephadex g-100 column. the molecular mass of the purified xylanse was about 33 kda. the enzyme was an endoxylanase as it initially degraded xylan to xylooligomers. the purified enzyme showed optimum activity at 55 degrees c and at ph 7.0 and rema ...201121790026
effects of photoactivated titanium dioxide nanopowders and coating on planktonic and biofilm growth of pseudomonas aeruginosa.we exploited the ability of photocatalytic titanium dioxide (tio(2) ) as an agent for the biofilm control. two photocatalytic systems were investigated: a 3g/l suspension of tio(2) nanopowder in demineralised water and glass slides coated with a tio(2) thin film, achieved by sol-gel deposition. a running protocol for the photoactivation of tio(2) was set up using the dye rhodamine b. the microorganisms studied were pseudomonas stutzeri, pseudomonas aeruginosa and a bacillus cereus-group as plank ...201121790617
design and synthesis of novel antimicrobial acyclic and heterocyclic dyes and their precursors for dyeing and/or textile finishing based on 2-n-acylamino-4,5,6,7-tetrahydro-benzo[b]thiophene systems.a series of novel polyfunctionalized acyclic and heterocyclic dye precursors and their respective azo (hydrazone) counterpart dyes and dye precursors based on conjugate enaminones and/or enaminonitrile moieties were synthesized. the dyes and their precursors are based on 2-cyano-n-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide, 2-ethoxycarbonyl-n-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide or 2-phenylcarbamoyl-n-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-aceta ...201121792147
synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents.a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ...201121794959
in vitro antimicrobial and antioxidant activity of acetone and methanol extracts from thymus leucotrichius (lamiaceae).thymus species has been used as tonic and herbal tea, antiseptic, antitussive, carminative, antioxidant, anti-inflammatory and antimicrobial activities. the acetone and methanol extracts of thymus (t.) leucotrichius (labiatae/lamiaceae) was examined for antimicrobial and antioxidant properties.201121796869
[isolation, identification and characterization of cypermethrin-degrading strain l12].to isolate and characterize bacteria to degrade cypermethrin (cp).201121796986
inhibition of bacillus cereus spore outgrowth and multiplication by chitosan.bacillus cereus is an endospore-forming bacterium able to cause food-associated illness. different treatment processes are used in the food industry to reduce the number of spores and thereby the potential of foodborne disease. chitosan is a polysaccharide with well-documented antibacterial activity towards vegetative cells. the activity against bacterial spores, spore germination and subsequent outgrowth and growth (the latter two events hereafter denoted (out)growth), however, is poorly docume ...201121798612
structural analysis of papain-like nlpc/p60 superfamily enzymes with a circularly permuted topology reveals potential lipid binding sites.nlpc/p60 superfamily papain-like enzymes play important roles in all kingdoms of life. two members of this superfamily, lrat-like and yaef/yiix-like families, were predicted to contain a catalytic domain that is circularly permuted such that the catalytic cysteine is located near the c-terminus, instead of at the n-terminus. these permuted enzymes are widespread in virus, pathogenic bacteria, and eukaryotes. we determined the crystal structure of a member of the yaef/yiix-like family from bacill ...201121799766
antimicrobial activity of mangrove plant (lumnitzera littorea).to investigate the antimicrobial activities of n-hexane, ethyl acetate and methanol extracts of the leaves of lumnitzera littorea (l. littorea) against six human pathogenic microbes.201121803301
microbial control of food-related surfaces: na-chlorophyllin-based photosensitization.the aim of this study was to evaluate efficiency of photosensitization as surface sanitation alternative using model systems when food pathogens, their spores and biofilms were attached to the food-related surface (polyolefine). in addition it was important to compare antibacterial efficiency of na-chlorophyllin (na-chl)-based photosensitization with conventional sanitizers. obtained results indicate that bacilluscereus atcc 12826 and listeriamonocytogenes atcc 7644 as well as their thermoresist ...201121807530
the chemical composition and antimicrobial activity of the leaf oil of cupressus lusitanica from monteverde, costa rica.the essential oils from the leaves of three different individuals of cupressus lusitanica were obtained by hydrodistillation and analyzed by gas chromatography - mass spectrometry. a total of 49 compounds were identified in the leaf oils. the major components of c. lusitanica leaf oil were +¦-pinene (40%-82%), limonene (4%-18%), isobornyl acetate (up to 10%) and cis-muurola-4(14),5-diene (up to 7%). the essential oil was screened for antimicrobial activity, and it showed antibacterial activity a ...201021808533
antimicrobial screening of ethnobotanically important stem bark of medicinal plants.the stem barks are the rich sources of tannins and other phenolic compounds. tannins inhibited the growth of various fungi, yeast, bacteria and virus. hence, ten stem barks of ethnomedicinally important plants were screened for antibacterial and antifungal activities against human pathogenic strains.201021808577
prevalence of bacillus cereus bacteriophages in fermented foods and characterization of phage jbp901.bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. in this study, the prevalence of b. cereus bacteriophages in korean fermented foods was studied using 6 reference strains, and jbp901 was further characterized. in a total of 47 samples, 19 (40.4%) contained more than one kind of b. cereus phage. fourteen phages were purified and determined for host lysis profile. among them, jbp901 was specific to b. cereus group s ...201121810470
genetic diversity of emetic toxin producing bacillus cereus korean strains.bacillus cereus is divided into emetic and enterotoxin producing strains. emetic b. cereus showed the low level of genetic diversity and single evolutionary lineage but no specific study of the genotypic characterization of emetic b. cereus korean strains has been conducted. the objective of this study was to investigate the genotypic diversity of emetic b. cereus korean strains. a total 39 strains (35 clinical and 4 food isolates) was analyzed for the genotypic characterization. a total of 17 d ...201121820749
evolutionary history and functional characterization of three large genes in bacillus cereus group bacteria involved in sporulation.the bacillus cereus group of bacteria is a group of closely related species that are of medical and economic relevance, including b. anthracis, b. cereus, and b. thuringiensis. bacteria from the bacillus cereus group encode three large, highly conserved genes of unknown function (named crda, crdb and crdc) that are composed of 16-35 copies of a repeated domain of 132 amino acids at the protein level. bioinformatic analysis revealed that there is a phylogenetic bias in the genomic distribution of ...201121821775
polyhydroxyalkanoate (pha) synthesis by class iv pha synthases employing ralstonia eutropha phb(-)4 as host strain.class iv polyhydroxyalkanoate (pha) synthase from bacillus cereus yb-4 (pharc(yb4)) or b. megaterium nbrc15308(t) (pharc(bm)) was expressed in ralstonia eutropha phb(-)4 to compare the ability to produce pha and the substrate specificity of pharcs. pharc(yb4) produced significant amounts of pha and had broader substrate specificity than pharc(bm).201121821924
investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.).the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ...201121822040
Rapidly Progressive, Fatal, Inhalation Anthraxlike Infection in a Human: Case Report, Pathogen Genome Sequencing, Pathology, and Coordinated Response.Context.-Ten years ago a bioterrorism event involving Bacillus anthracis spores captured the nation's interest, stimulated extensive new research on this pathogen, and heightened concern about illegitimate release of infectious agents. Sporadic reports have described rare, fulminant, and sometimes fatal cases of pneumonia in humans and nonhuman primates caused by strains of Bacillus cereus , a species closely related to Bacillus anthracis . Objectives.-To describe and investigate a case of rapid ...201121827220
composition and in-ávitro antibacterial activity of essential oils from four satureja species growing in iran.the composition and in-ávitro antibacterial activity of essential oils from four satureja species (s. bachtiarica, s. khuzistanica, s. mutica and s. rechingeri) growing in iran were determined. according to the results of gc-fid and gc-ms analysis, all oil samples were principally composed of phenolic constituents (carvacrol and/or thymol) with the percentages ranging from 41.2% (s. bachtiarica) to 77.7% (s. rechingeri). determining antibacterial activities by the disc diffusion method against t ...201121827283
chemical constituents and antibacterial activity of melastoma malabathricum l.the aqueous methanolic extracts of melastoma malabathricum l. exhibited antibacterial activity when assayed against seven microorganisms by the agar diffusion method. solvent fractionation afforded active chloroform and ethyl acetate fractions from the leaves and the flowers, respectively. a phytochemical study resulted in the identification of ursolic acid (1), 2+¦-hydroxyursolic acid (2), asiatic acid (3), +¦-sitosterol 3-o-+¦-d-glucopyranoside (4) and the glycolipid glycerol 1,2-dilinolenyl-3 ...201121834640
treatment of experimental bacillus cereus endophthalmitis using intravitreal moxifloxacin with or without dexamethasone.abstract purpose: to evaluate the effects of intravitreal moxifloxacin with or without dexamethasone on experimental bacillus cereus endophthalmitis. methods: the right eyes of 21 new zealand albino rabbits weighing 2-3 kg were used in this study. each eye was inoculated with 1+ù10(6) colony-forming units of b. cereus microorganisms via intravitreal injection into the vitreous cavity, and an experimental model of b. cereus endophthalmitis was formed. the rabbits were separated into 3 groups: gro ...201121834669
attachment strength and biofilm forming ability of bacillus cereus on green-leafy vegetables: cabbage and lettuce.the present study was designed to investigate the ability of six bacillus cereus strains to attach and form biofilm on cabbage and lettuce surfaces. these six strains were; a reference strain dsmz 345 and five biofilm-producing strains (aquatic strains; tub8, tub30, tub31, tub32 and tub33) isolated from drinking-water distribution network. hydrophobicity, biofilm formation ability, attachment strength (s(r)) of spores and vegetative cells of the six b.-ácereus strains were also determined. due t ...201121839375
incidence, diversity and toxin gene characteristics of bacillus cereus group strains isolated from food products marketed in belgium.the major objectives of this study were to determine the incidence, diversity and characteristics of bacillus cereus group spp. isolated from food products marketed in belgium. the food products investigated in this study included cooked pasta, lasagna, b+®chamel sauce, bolognaise sauce, fresh minced beef, fresh-cut vegetables and raw basmati rice. b. cereus group spp. were detected in 56.3% (324 of 575) of the samples giving rise to 380 strains. the highest incidence (100%) occurred in the raw ...201121840614
controlled delivery of gentamicin using poly(3-hydroxybutyrate) microspheres.poly(3-hydroxybutyrate), p(3hb), produced from bacillus cereus spv using a simple glucose feeding strategy was used to fabricate p(3hb) microspheres using a solid-in-oil-water (s/o/w) technique. for this study, several parameters such as polymer concentration, surfactant and stirring rates were varied in order to determine their effect on microsphere characteristics. the average size of the microspheres was in the range of 2 ++m to 1.54 ++m with specific surface areas varying between 9.60 m(2)/g ...201121845079
Antibacterial activity of different degree of hydrolysis of palm kernel expeller peptides against spore-forming and non-spore-forming bacteria.The goal of this study was to determine inhibitory effect of palm kernel expeller (PKE) peptides of different degree of hydrolysis (DH %) against spore-forming bacteria Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophillus, Bacillus subtilis, Bacillus thuringiensis, Clostridium perfringens; and non-spore-forming bacteria Escherichia coli, Lisinibacillus sphaericus, Listeria monocytogenes, Pseudomonas a ...201121848644
2,4,6-trinitrophenol degradation by bacillus cereus isolated from a firing range.bacillus cereus strain pu, isolated from soil contaminated with explosive waste, tolerated up to 1.3 mm 2,4,6 trinitrophenol (tnp) and utilize it aerobically as sole nitrogen and carbon source. degradation of tnp was accompanied by stoichiometric release of 2.1 ± 0.15 mol nitrite/mol tnp at 539 μmol/h g dry cell wt. metabolism of tnp was accompanied by transient accumulation of an orange-red metabolite, hydride meisenheimer complex (h-tnp), indicating a metabolic pathway involving complete reduc ...201121858669
antibacterial properties of a glycolipid-rich extract and active principle from nunavik collections of the macroalgae fucus evanescens c. agardh (fucaceae).this study investigated the antibacterial activity of glycolipid-rich extracts of the brown macroalga fucus evanescens in cell culture. accessions were collected on the arctic coast of ungava bay, nunavik, quebec. the crude ethyl acetate extract of these accessions showed strong antibacterial activity (≥4 log(10) cfu) against hemophilus influenzae , legionella pneumophila , propionibacterium acnes (atcc and clinical isolate), and streptococcus pyogenes at 100 µg/ml. this algal extract inhibited ...201121859295
Biochemical characteristics and biological properties of annurca apple cider.Abstract Our work aimed to investigate the phenolic composition and antioxidant capacity of the de-alcoholized extract of cider obtained from the Annurca apple (Malus domestica var. Annurca). The antimicrobial effect of the extract against different pathogens, including Chronobacter sakazakii, was also examined. The extract's potential anti-quorum-sensing (AQS) activity was assessed using the test microorganism Chromobacterium violaceum. Biochemical analysis of the extract using ultra-performa ...201121861727
coriander (coriandrum sativum l.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry.the aim of this work was to study the antibacterial effect of coriander (coriandrum sativum) essential oil against gram-positive and gram-negative bacteria. antibacterial susceptibility was evaluated using classical microbiological techniques concomitantly with the use of flow cytometry for the evaluation of cellular physiology. our results showed that coriander oil has an effective antimicrobial activity against all bacteria tested. also, coriander oil exhibited bactericidal activity against al ...201121862758
Whole-genome phylogenies of the family Bacillaceae and expansion of the sigma factor gene family in the Bacillus cereus species-group.The Bacillus cereus sensu lato group consists of six species (B. anthracis, B. cereus, B. mycoides, B. pseudomycoides, B. thuringiensis, and B. weihenstephanensis). While classical microbial taxonomy proposed these organisms as distinct species, newer molecular phylogenies and comparative genome sequencing suggests that these organisms should be classified as a single species (thus, we will refer to these organisms collectively as the Bc species-group). How do we account for the underlying simil ...201121864360
Chemical and microbiological hazards associated with recycling of anaerobic digested residue intended for agricultural use.In the present study, three full-scale biogas plants (BGP) were investigated for the concentration of heavy metals, organic pollutants, pesticides and the pathogenic bacteria Bacillus cereus and Escherichia coli in the anaerobically digested residues (ADR). The BGPs mainly utilize source-separated organic wastes and industrial food waste as energy sources and separate the ADR into an ADR-liquid and an ADR-solid fraction by centrifugation at the BGP. According to the Norwegian standard for organi ...201121865025
fluorogenic cephalosporin substrates for β-lactamase tem-1.cephalosporin was used to synthesize soluble and precipitating fluorogenic β-lactam substrates that demonstrated differential catalytic hydrolysis by three different subtypes of β-lactamase: tem-1 (class a), p99 (class c), and a bacillus cereus enzyme sold by genzyme (class b). the most successful soluble substrate contained difluorofluorescein (oregon green 488) ligated to two cephalosporin moieties that, therefore, required two turnovers to produce the fluorescent oregon green 488 leaving grou ...201121867672
Isolation and characterization of aerobic culturable arsenic-resistant bacteria from surfacewater and groundwater of Rautahat District, Nepal.Arsenic (As) contamination of groundwater is a serious Environmental Health Management issue of drinking water sources especially in Terai region of Nepal. Many studies have reported that due to natural abundance of arsenic in the environment, various bacteria have developed different resistance mechanisms for arsenic compound. In this study, the culturable arsenic-resistant bacteria indigenous to surfacewater as well as groundwater from Rautahat District of Nepal were randomly isolated by stand ...201121868146
heat stress leads to superoxide formation in bacillus cereus detected using the fluorescent probe mitosox.bacillus cereus is a food-borne human pathogen and food spoilage organism. spores and vegetative cells of b. cereus can be found almost everywhere and therefore often end up in food processing equipment and food products. to remove spores and vegetative cells from food or equipment, harsh treatments such as high temperatures are applied. the heat stress response of b. cereus and other organisms has been studied and it has been shown that reactive oxygen species may be involved in inactivating th ...201121872960
de-mercurization of wastewater by bacillus cereus (jubt1): growth kinetics, biofilm reactor study and field emission scanning electron microscopic analysis.removal of mercuric ions by a mercury resistant bacteria, called bacillus cereus (jubt1), isolated from the sludge of a local chlor-alkali industry, has been investigated. growth kinetics of the bacteria have been determined. a multiplicative, non-competitive relationship between sucrose and mercury ions has been observed with respect to bacterial growth. a combination of biofilm reactor, using attached growth of bacillus cereus (jubt1) on rice husk packing, and an activated carbon filter has be ...201121872987
Bovine intestinal bacteria inactivate and degrade ceftiofur and ceftriaxone with multiple beta-lactamases.The veterinary cephalosporin drug ceftiofur is rapidly degraded in the bovine intestinal tract. A cylinder-plate assay was used to detect microbiologically active ceftiofur, and high-performance liquid chromatography-mass spectrometry analysis was used to quantify the amount of ceftiofur remaining after incubation with bovine intestinal anaerobic bacteria, which were isolated from colon contents or feces from 8 cattle. Ninety-six percent of the isolates were able to inactivate ceftiofur to some ...201121876048
antibacterial activity of three medicinal thai plants against campylobacter jejuni and other foodborne pathogens.leaves of adenanthera pavonina, moringa oleifera and annona squamosa are used in traditional thai medicine to treat dysentery and other diseases. this study investigated the antibacterial activity of these plants against six species of foodborne pathogen. methods and solvents employed to extract active constituents were optimised using the disc diffusion assay. phytochemical analysis of the optimised extracts was performed by thin layer chromatography (tlc). minimum inhibitory concentrations (mi ...201121878033
the potential for reductive mobilization of arsenic [as(v) to as(iii)] by osbh(2) (pseudomonas stutzeri) and osbh(5) (bacillus cereus) in an oil-contaminated site.microbial reduction of arsenate [as(v)] plays an important role in arsenic (as) mobilization in aqueous environments. in this study, we investigated reduction of arsenate by different bacterial isolates such as osbh(1) (gu329913), osbh(2) (gu329914), osbh(3) (gu329915), osbh(4) (gu329916) and osbh(5) (gu329917), isolated from the oil sludge of a sewage treatment plant operated by the china petroleum refinery company in kaohsiung, southern taiwan. bacterial strains of pure culture were identified ...201121879856
Biotransformation of puerarin into puerarin-6?-O-phosphate by Bacillus cereus.The biotransformation of puerarin catalyzed by Bacillus cereus NT02 was studied. A primary screening was carried out using 307 strains of bacteria isolated from soil which were able to grow in the presence of puerarin. Strain NT02, identified as B. cereus, was able to convert puerarin into puerarin-6?-O-phosphate. Under the optimum conditions, resting cells of B. cereus NT02 converted 27% of added 0.4 g/l puerarin into puerarin-6?-O-phosphate that was characterized by MS, (13)C NMR, (31)P NMR. T ...201121882014
Rapidly progressive, fatal, inhalation anthrax-like infection in a human: case report, pathogen genome sequencing, pathology, and coordinated response.Ten years ago a bioterrorism event involving Bacillus anthracis spores captured the nation's interest, stimulated extensive new research on this pathogen, and heightened concern about illegitimate release of infectious agents. Sporadic reports have described rare, fulminant, and sometimes fatal cases of pneumonia in humans and nonhuman primates caused by strains of Bacillus cereus , a species closely related to Bacillus anthracis.201121882964
microbial contamination of food products consumed by infants and babies in korea.the objectives of this study were to investigate the microbiological safety of various foods intended for consumption by infants and babies.201121883321
biosynthesis of cyclodextrin glucosyltransferase by the free and immobilized cells of bacillus cereus nrc7 in batch and continuous cultures.the objective of this study was to enhance the production of cyclodextrin glucanotransferase (cgtase) produced by a local isolate bacillus cereus nrc7.201121883731
ywdl in bacillus cereus: its role in germination and exosporium structure.in members of the bacillus cereus group the outermost layer of the spore is the exosporium, which interacts with hosts and the environment. efforts have been made to identify proteins of the exosporium but only a few have so far been characterised and their role in determining spore architecture and spore function is still poorly understood. we have characterised the exosporium protein, ywdl. δywdl spores have a more fragile exosporium, subject to damage on repeated freeze-thawing, although ther ...201121887322
A novel pathogen-binding gC1qR homolog, FcgC1qR, in the Chinese white shrimp, Fenneropenaeus chinensis.In vertebrates, the globular "head" of complement component C1q receptor (gC1qR) is a versatile, multiligand binding protein. However, research on its function in invertebrates is limited. In the present study, a full-length cDNA sequence of a novel gC1qR homolog, FcgC1qR, from the Chinese white shrimp Fenneropenaeus chinensis was cloned. Semi-quantitative polymerase chain reaction (PCR) detected FcgC1qR in all examined tissues, with the highest level detected in the intestine. Western blot assa ...201221893092
surface architecture of endospores of the bacillus cereus/anthracis/thuringiensis family at the subnanometer scale.bacteria of the bacillus cereus family form highly resistant spores, which in the case of the pathogen b. anthracis act as the agents of infection. the outermost layer, the exosporium, enveloping spores of the b. cereus family as well as a number of clostridia, plays roles in spore adhesion, dissemination, targeting, and germination control. we have analyzed two naturally crystalline layers associated with the exosporium, one representing the "basal" layer to which the outermost spore layer ("ha ...201121896762
microbial examination of nonheated foods served in feeding programs of elementary schools, iksan city, jeonbuk province, korea.more than 90% of elementary school students in korea have lunch provided by a school feeding program. this study examined nonheated foods, foods in which final ingredients were added after cooking ("heated/nonheated foods"), and desserts for microbial contamination levels and the presence of foodborne pathogens. we obtained a total of 77 food samples belonging to the above three groups from four elementary schools located in iksan, jeonbuk, korea, from june to july 2010. among the samples, 15% o ...201121902929
chitosan and silver nanoparticles as pudding with raisins with antimicrobial properties.chitosan nanoparticles (cs-np) containing small silver nanoparticles are reported (ag@cs-np). cs-np was synthesized using tripolyphosphate (tpp) as a polyanionic template. tpp also served to electrostatically attract ag(+) inside cs-np, where it was reduced by the terminal glucosamine units of the biopolymer. this procedure is environmental friendly, inexpensive, and permits the synthesis of very small agnp (0.93-1.7 nm), with only a discrete dependence from the amount of silver nitrate used (5- ...201121903218
An analysis of temperature adaptation in cold active, mesophilic and thermophilic Bacillus a-amylases.A comparative biochemical and structural study was performed on a cold active a-amylase from Bacillus cereus (BCA) and two well-known homologous mesophilic and thermophilic a-amylases from Bacillus amyloliquefaciens (BAA) and Bacillus licheniformis (BLA). In spite of a high degree of sequence and structural similarity, drastic variations were found for T(opt) as 50, 70 and 90°C for BCA, BAA and BLA, respectively. The half-lives of thermoinactivation were 1 and 9 min for BCA and BAA at 80°C respe ...201121907234
identification of a bacillus anthracis specific indel in the yeac gene and development of a rapid pyrosequencing assay for distinguishing b. anthracis from the b. cereus group.bacillus anthracis, the causative agent of anthrax, is a potential source of bioterrorism. the existing assays for its identification lack specificity due to the close genetic relationship it exhibits to other members of the b. cereus group. our comparative analyses of protein sequences from bacillus species have identified a 24 amino acid deletion in a conserved region of the yeac protein that is uniquely present in b. anthracis. pcr primers based on conserved regions flanking this indel in the ...201121907250
protection activity of a novel probiotic strain of bacillus subtilis against salmonella enteritidis infection.the activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. in observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including salmonella enteritidis, salmonella typhimurium, escherichia co ...201121907374
trypan blue dye enters viable cells incubated with the pore-forming toxin hlyii of bacillus cereus.trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. here, we show that the pore-forming toxin hlyii of bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. these findings suggest that the dye enters viable cells through the pores. to our knowledge, this is the first demonstration that trypan blue may enter viable cells. consequently, the use of trypan blue staining as a marker of ...201121909398
preparation and antimicrobial activity of poly (vinyl chloride)/gelatin/montmorillonite biocomposite films.the aim of this study was using a novel antimicrobial thermoplastic plasticizer based on aliphatic anhydride derivative dodecenyl succinic anhydride (dsa) for blending poly (vinyl chloride), pvc, with gelatin in presence of montmorillonite (mmt) using brabender via polymer melting technique. this anhydride-based plasticizer blended the membrane ingredients homogenously under melting process. the used plasticizer exhibited high performance antimicrobial potency for some biomedical and industrial ...201121909641
a multivalent probe for ai-2 quorum-sensing receptors.multivalency is a common principle in the recognition of cellular receptors, and multivalent agonists and antagonists have played a major role in understanding mammalian cell receptor biology. the study of bacterial cell receptors using similar approaches, however, has lagged behind. herein we describe our efforts toward the development of a dendrimer-based multivalent probe for studying ai-2 quorum-sensing receptors. from these studies, we have discovered a chemical probe specific for lsr-type ...201121913711
Demonstration of Bacillus cereus in orthopaedic-implant-related infection with use of a multi-primer polymerase chain reaction-mass spectrometric assay: report of two cases. 201121915528
Composition and antimicrobial activity of Seseli globiferum essential oil.The essential oil from aerial parts of Seseli globiferum Vis. obtained by hydrodistillation with Clevenger-type apparatus was analyzed by GC-MS. Twenty-eight compounds were identified, representing 99.4% of the total oil. The main components of the oil were sabinene (38.0%), alpha-pinene (21.2%) and beta-phellandrene (13.5%). The microbial growth inhibitory properties of the isolated essential oil were determined using the broth microdilution method against seven bacterial species: Salmonella ty ...201121922927
bioremoval of trivalent chromium using bacillus biofilms through continuous flow reactor.present study deals with the applicability of bacterial biofilms for the bioremoval of trivalent chromium from tannery effluents. a continuous flow reactor was designed for the development of biofilms on different substrates like glass beads, pebbles and coarse sand. the parameters for the continuous flow reactor were 20 ml/min flow rate at 30°c, ph4. biofilm biomass on the substrates was in the following sequence: coarse sand>pebbles>glass beads (4.8 × 10(7), 4.5 × 10(7) and 3.5 × 10(5)cfu/cm(2 ...201121924829
formation of cereulide and enterotoxins by bacillus cereus in fermented african locust beans.afitin, iru and sonru are three spontaneously fermented african locust bean benin condiments. the fermentation processes are exothermic, with temperatures mostly being above 40 °c. a total of 19 predominant bacillus cereus isolates from afitin, iru and sonru, were investigated. the enterotoxin genes nhe (a, b, c) were present in all 19 isolates, the hbl (a, c, d) in one (afitin), and the cytk gene in three isolates (afitin). levels of cytotoxicity to vero cells and nhea production in bhi-broth w ...201121925026
β-lysine discrimination by lysyl-trna synthetase.elongation factor p is modified with (r)-β-lysine by the lysyl-trna synthetase (lysrs) paralog poxa. poxa specificity is orthogonal to lysrs, despite their high similarity. to investigate α- and β-lysine recognition by lysrs and poxa, amino acid replacements were made in the lysrs active site guided by the poxa structure. a233s lysrs behaved as wild type with α-lysine, while the g469a and a233s/g469a variants decreased stable α-lysyl-adenylate formation. a233s lysrs recognized β-lysine better th ...201121925499
Transcriptional responses of Bacillus cereus towards challenges with the polysaccharide chitosan.The antibacterial activity of the polysaccharide chitosan towards different bacterial species has been extensively documented. The response mechanisms of bacteria exposed to this biopolymer and the exact molecular mechanism of action, however, have hardly been investigated. This paper reports the transcriptome profiling using DNA microarrays of the type-strain of Bacillus cereus (ATCC 14579) exposed to subinhibitory concentrations of two water-soluble chitosan preparations with defined chemical ...201121931677
antimicrobial activities of hydrogen peroxide and its activation by a novel heterogeneous fenton's-like modified pan catalyst.to investigate the potential activation of hydrogen peroxide by a novel catalyst, reducing the concentration of hydrogen peroxide required and the time taken for microbial inactivation.201121933313
Efficacy of intravascular catheter lock solutions containing preservatives in the prevention of microbial colonization.There is little published evidence regarding whether heparin lock solutions containing preservatives prevent catheter-related infections. However, adverse effects from preservative-containing flushes have been documented in neonates, leading many hospitals to avoid their use altogether. Infection control records from 1982 to 2008 at St. Jude Children's Research Hospital (SJCRH) were reviewed regarding the incidence of catheter-related infections and the use of preservative-containing intravenous ...201121945067
Fluorinated s-triazinyl piperazines as antimicrobial agents.A series of 1,3,5-triazine derivatives that contain 4-amino-2-trifluoromethyl-benzonitrile, 8-hydroxyquinoline, and different piperazines as substituents at the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. The chemical structures of the compounds were elucidated with the aid of IR, 1H NMR and 13C NMR spectroscopy, and elemental analysis. The antimicrobial activity of the compounds was tested against seven bacteria (Staphylococcus aureus MT ...201121950158
Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar.Phosphatase Producing Bacteria (PPB) and Inorganic Phosphate Solubilizing Bacteria (IPSB) are important to reef nutrition. These microbes and phosphate concentration play a significant role in the productivity of coral reef ecosystems. A study was conducted in Gulf of Mannar coral reef ecosystem to understand the diversity of these groups of bacteria and their competence in mineralizing the phosphate. The PPB isolates were identified under six genera i.e. Bacillus, Pseudomonas, Micrococcus, Vibr ...201121953521
enzymes acting on d-amino acid containing peptides.using a synthetic oligopeptide (d-phe)(4), a microorganism bacillus cereus df4-b producing alkaline d-peptidase (adp) was isolated. the enzymatic properties have been characterized; the enzyme showed d-stereospecific dipeptidyl aminopeptidase and endopeptidase activities. the enzyme was active toward (d-phe)(n), boc-(d-phe)(n), (d-phe)(n) methyl ester, d-phe-nh(2), boc-(d-phe)(n) methyl ester, and boc-(d-phe)(n) tert-butyl ester, but not toward (d-ala)(n) (n = 2-4), (d-val)(3), and (d-leu)(2).201221956579
a cell-cell communication system regulates protease production during sporulation in bacteria of the bacillus cereus group.in sporulating bacillus, major processes like virulence gene expression and sporulation are regulated by communication systems involving signalling peptides and regulators of the rnpp family. we investigated the role of one such regulator, nprr, in bacteria of the bacillus cereus group. we show that nprr is a transcriptional regulator whose activity depends on the nprx signalling peptide. in association with nprx, nprr activates the transcription of an extracellular protease gene (npra) during t ...201121958299
phytochemical constituents and antibacterial efficacy of the flowers of peltophorum pterocarpum (dc.) baker ex heyne.to investigate the preliminary phytochemistry and antibacterial activity of the flower extract of peltophorum pterocarpum .201121967698
re-pcr variability and toxigenic profile of food poisoning, foodborne and soil-associated bacillus cereus isolates from brazil.twenty-three bacillus cereus isolates from food poisoning outbreaks associated with a diarrheal-type syndrome, fourteen foodborne isolates not associated with food poisoning and fifteen isolates from brazilian soil samples were analyzed for the presence and genetic diversity (by re-pcr) of the virulence genes ces (emetic toxin, cereulide), plcr-papr (pleiotropic regulator plcr and peptide papr), nhea (a component of the nhe complex), bcet (diarrheal enterotoxin bc-d-ent), gyrb (b subunit of dna ...201121978657
effect of povidone-iodine concentration and exposure time on bacteria isolated from endophthalmitis cases.to determine the in vitro susceptibility of post-cataract surgery endophthalmitis bacterial isolates to different concentrations of povidone-iodine at different exposure times.201121983301
molecular architecture of a multifunctional mcm complex.dna replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. one of them is the replicative helicase, which is required for initiation and elongation phases. a mcm helicase found as a prophage in the genome of bacillus cereus is fused with a primase domain constituting an integrative arrangement of two essential activities for replication. we have isolated this helicase-primase complex (bcmcm) showing that it can bind dna and displays not ...201121984415
diversity and bioactive potential of endospore-forming bacteria cultured from the marine sponge haliclona simulans.despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. the main aims of this study were to isolate and characterize the spore-forming bacteria from the marine sponge haliclona simulans and to examine their potential as a source for bioactive compounds.201221985154
[contamination by bacillus cereus on equipment and utensil surfaces in a food and nutrition service unit].the confirmation that bacillus cereus is a microorganism that represents a special problem in food processing plants, such as university cafeterias, inspired this work, the scope of which was to evaluate the risks consumers are exposed to by identifying the contamination points from whence the microorganism can be transferred on to food. the presence of b. cereus was detected in 38.3% of the equipment and utensils studied. counts of up to 5.7 x 10 ² cfu/cm² were found, with the highest values be ...201121987337
chemical composition and antimicrobial activity of essential oil of achillea cretica l. (asteraceae) from turkey.hydrodistilled volatile oil from the aerial parts of achillea cretica l. (asteraceae) was analysed by a combination of gc and gc/ms. seventy-six components were identified, constituting 86.4% of the oil. the main constituents of the essential oil were caryophylladienol-ii (13.4%), β-maaliene (6.1%), neo-intermedeol (6.0%), carvone (4.9%), spathulenol (4.5%), palmitic acid (3.3%) and selina-3,11-dien-6α-ol (3.2%). the antimicrobial activity was evaluated by the broth-dilution method on nine micro ...201121988483
antibacterial characteristics and activity of water-soluble chitosan derivatives prepared by the maillard reaction.the antibacterial activity of water-soluble chitosan derivatives prepared by maillard reactions against staphylococcus aureus, listeria monocytogenes, bacillus cereus, escherichia coli, shigella dysenteriae, and salmonella typhimurium was examined. relatively high antibacterial activity against various microorganisms was noted for the chitosan-glucosamine derivative as compared to the acid-soluble chitosan. in addition, it was found that the susceptibility of the test organisms to the water-solu ...201121989311
[analyze and compare metabolic pathways of bacillus cereus group].a large number of data and information was obtained from genome sequencing and high-throughput genomic studies, use of the information to study metabolic networks become a new hotspot in biological research. this article compared different methods to reconstruct metabolic networks and analyzed the advantages and disadvantages of each methods, and then introduced some researches about carbohydrate metabolism pathways, amino acid metabolic pathways, and energy metabolism pathways of 9 strains of b ...201121993280
biodegradation potential of oily sludge by pure and mixed bacterial cultures.the biodegradation capacity of aliphatic and aromatic hydrocarbons of petrochemical oily sludge in liquid medium by a bacterial consortium and five pure bacterial cultures was analyzed. three bacteria isolated from petrochemical oily sludge, identified as stenotrophomonas acidaminiphila, bacillus megaterium and bacillus cibi, and two bacteria isolated from a soil contaminated by petrochemical waste, identified as pseudomonas aeruginosa and bacillus cereus demonstrated efficiency in oily sludge d ...201121993328
From transcriptional landscapes to the identification of biomarkers for robustness.The ability of microorganisms to adapt to changing environments and gain cell robustness, challenges the prediction of their history-dependent behaviour. Using our model organism Bacillus cereus, a notorious Gram-positive food spoilage and pathogenic spore-forming bacterium, a strategy will be described that allows for identification of biomarkers for robustness. First an overview will be presented of its two-component systems that generally include a transmembrane sensor histidine kinase and it ...201121995521
Bacterial inactivation using silver-coated magnetic nanoparticles as functional antimicrobial agents.The ability for silver nanoparticles to function as an antibacterial agent while being separable from the target fluids is important for bacterial inactivation in biological fluids. This report describes the analysis of the antimicrobial activities of silver-coated magnetic nanoparticles synthesized by wet chemical methods. The bacterial inactivation of several types of bacteria was analyzed, including Gram-positive bacteria ( Staphylococcus aureus and Bacillus cereus ) and Gram-negative bacte ...201121999710
A hospital acquired outbreak of Bacillus cereus gastroenteritis, Oman.To investigate the course of a hospital acquired outbreak of Bacillus cereus gastroenteritis outbreak, and the interventions that were taken to prevent such an outbreak from occurring again.201122000845
Validated empirical models describing the combined effect of water activity and pH on the heat resistance of spores of a psychrotolerant Bacillus cereus strain in broth and béchamel sauce.The major objective of this study was to evaluate and model the combined effect of the water activity (a(w)) and pH of the heating menstrum on the heat resistance of spores of a psychrotolerant Bacillus cereus strain isolated from béchamel sauce. Two models, a quadratic polynomial equation and a reparameterized function, were assessed for their ability to describe the combined influence of a(w) and pH on the D(85°C)-values of the B. cereus isolate in tryptone soy broth. The performance of the m ...201122004813
defense role of the cocoon in the silk worm bombyx mori l.silk from the domesticated silk worm bombyx mori procures foreign body response naturally, so it has been utilized as a biomaterial for decades. in india the prime focus of the sericulture industry is to improve silk production with high quality silk. naturally, the silk worm builds its cocoon not only with silk proteins, but also with antimicrobial proteins to avoid infection since the cocoon is non-motile and non-feeding. the aim of the present study is to elucidate the antimicrobial proteins ...201122006381
sudden death of a young adult associated with bacillus cereus food poisoning.a lethal intoxication case, which occurred in brussels, belgium, is described. a 20-year-old man died following the ingestion of pasta contaminated with bacillus cereus. emetic strains of b. cereus were isolated, and high levels of cereulide (14.8 μg/g) were found in the spaghetti meal.201122012017
antimicrobial activity, cytotoxicity, and phytochemical screening of voacanga globosa (blanco) merr. leaf extract (apocynaceae).to determine the antibacterial, antifungal, antiprotozoal, cytotoxic, and phytochemical properties of ethanol extracts of leaves of voacanga globosa (blanco) merr. (v. globosa).201122014741
diversity of bacterial communities that colonize the filter units used for controlling plant pathogens in soilless cultures.in recent years, increasing the level of suppressiveness by the addition of antagonistic bacteria in slow filters has become a promising strategy to control plant pathogens in the recycled solutions used in soilless cultures. however, knowledge about the microflora that colonize the filtering columns is still limited. in order to get information on this issue, the present study was carried out over a 4-year period and includes filters inoculated or not with suppressive bacteria at the start of t ...201122015683
screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40.the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated.201222017666
complete genome sequence and characterization of the n-acylhomoserine lactone-degrading gene of the potato leaf-associated solibacillus silvestris.n-acylhomoserine lactones (ahls) are used as quorum-sensing signal molecules by many gram-negative bacteria. we have reported that solibacillus silvestris, which was isolated from the potato leaf, has ahl-degrading activity. to identify the ahl-degrading gene, whole genome sequencing of s. silvestris stlb046 was performed by using pyrosequencing technology. as the result of the blast search, one predicted orf (ahls) showed slight similarity to aiia-like ahl lactonase from bacillus cereus group. ...201122019407
Hyicin 3682, a bioactive peptide produced by Staphylococcus hyicus 3682 with potential applications for food preservation.Bacteriocins are peptides produced by bacteria and having inhibitory activity against other bacteria. Many of these substances may be useful as antibacterial agents for practical applications. In this study, 21 Staphylococcus spp. isolated from pigs, dogs and bovine milk in different states of Brazil were investigated for staphylococcin production. Hyicin 3682, a bacteriocin produced by one such strain, inhibited almost all strains tested, including Bacillus cereus, Listeria monocytogenes and St ...201122019494
Biosynthesis of UDP-glucuronic acid and UDP-galacturonic acid in Bacillus cereus subsp. cytotoxis NVH 391-98.The food borne pathogen Bacillus cereus produces uronic acid-containing glycans that are secreted in a shielding biofilm environment, and certain alkaliphilic Bacillus deposit uronate-glycan polymers in the cell wall when adapting to alkaline environments. The source of these acidic sugars is unknown and, in the present study, we describe the functional identification of an operon in Bacillus cerues subsp. cytotoxis NVH 391-98 that comprises genes involved in the synthesis of UDP-uronic acids in ...201222023070
Characterization of Type II and III Restriction-Modification Systems from Bacillus cereus Strains ATCC 10987 and ATCC 14579.The genomes of two Bacillus cereus strains (ATCC 10987 and ATCC 14579) have been sequenced. Here, we report the specificities of type II/III restriction (R) and modification (M) enzymes. Found in the ATCC 10987 strain, BceSI is a restriction endonuclease (REase) with the recognition and cut site CGAAG 24-25/27-28. BceSII is an isoschizomer of AvaII (G/GWCC). BceSIII cleaves at ACGGC 12/14. The BceSIII C terminus resembles the catalytic domains of AlwI, MlyI, and Nt.BstNBI. BceSIV is composed of ...201222037402
vigorous cleaning and adequate ventilation are necessary to control an outbreak in a neonatal intensive care unit.an outbreak of bacillus cereus (b. cereus) bacteremia occurred in our neonatal intensive care unit (nicu) in july 2005. many strains of b. cereus were cultured from patient specimens, as well as from environmental samples such as the surfaces of instruments and air in the nicu. some of these strains were analyzed by pulsed field gel electrophoresis, and several were confirmed to be identical. we speculated that the bacterial load in the environment had initially increased and then possibly sprea ...201122038125
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