Publications

single nucleotide polymorphisms in interleukin-1gene cluster and subgingival colonization with aggregatibacter actinomycetemcomitans in patients with aggressive periodontitis.	periodontitis is initiated by the subgingival occurrence of periodontopathogens. it is triggered by a specific host-dependent immune response that is influenced by genetic predisposition. polymorphisms in the interleukin-1 (il-1) gene cluster have been suggested to influence the pathogenesis of periodontitis. a total of 159 periodontitis patients (chronic disease: n = 73, aggressive disease: n = 86) and 89 periodontitis-free controls were included in the study. polymorphisms il-1╬▒ (rs1800587), ...	2011	21672595
antimicrobial sesquiterpenoids from laurus nobilis l.	activity-guided fractionations of leaf extracts from laurus nobilis l. led to the isolation of a known sesquiterpene lactone, deacetyl laurenobiolide (1). compound 1 showed antimicrobial activity against periopathic pathogens (actinomyces viscosus, porphyromonas gingivalis, prevotella intermedia and actinobacillus actinomycetemcomitans), opportunistic gram-positive bacteria (staphylococcus aureus and streptococcus pyogenes) and fungi (candida albicans, cryptococcus neoformans and aspergillus fum ...	2011	21678158
[prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis].	helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori.	2011	21688602
Tongue piercing: the effect of material on microbiological findings.	Biofilms on oral piercings may serve as a bacterial reservoir and lead to systemic bacteremia or local transmission of pathogenic microbiota. The use of piercing materials which are less susceptible to biofilm accumulation could contribute to prevention of problems. The present study investigated whether there are microbiological differences in bacterial samples collected from tongue piercings made of different materials.	2011	21700161
[analysis of the inhibitory capacity against bacterial growth of several materials of dental use. preliminar].	dental plaque is one of the reasons for odontogenic infection. although multiple bacteria are involved in these processes, a.actinomycetemcomitans and s.mutans are considered directly responsible for localized aggressive periodontitis and caries respectively. on the other hand, it seems clear that the oral flora utilizes especially not well polished surfaces or without bacteriastatic capacity in order to obtain better adhesion. based on those facts we expect to prove the inhibitory capacity "in ...	2011	21716233
[pcr "real time" to analyze the quantitative and qualitative relations microbiota of periodontal pockets].	the introduction of a broad medical practice pcr "real time" is just beginning and dentistry is no exception. modern molecular genetic methods provide numerous opportunities for diagnosis, assessment and prediction in patients with inflammatory periodontal diseases. early and accurate diagnosis can allow in the future reduce the incidence of periodontitis and the progression of its course.	2011	21716234
clinical, microbiologic, and immunologic factors of orthodontic treatment-induced gingival enlargement.	the aim of this study was to investigate the microbiologic and immunologic factors related to orthodontic treatment-induced gingival enlargement (ge).	2011	21724088
does routine analysis of subgingival microbiota in periodontitis contribute to patient benefit?	in clinical periodontology it is common practice to sample subgingival plaque from periodontitis patients and to search for the presence of alleged periodontal pathogens using routine laboratory techniques such as culture, dna-dna hybridization or real-time pcr. usually, special attention is given to the recognition of 'red complex' microorganisms and to aggregatibacter actinomycetemcomitans. recently, molecular open-ended techniques have been introduced which are distinct from the more 'classic ...	2011	21726285
live/dead real-time polymerase chain reaction to assess new therapies against dental plaque-related pathologies.	dna-based methodology for the identification and detection of specific bacteria in dental plaque offers advantages over culturing techniques. one drawback of current molecular techniques like real-time quantitative polymerase chain reaction (rt-qpcr) is that they are not able to distinguish between live or dead bacteria. to overcome this problem an assay was assessed to discriminate between viable or dead bacteria using dna intercalating substances, propidium monoazide (pma) and ethidium monoazi ...	2011	21729246
aggregatibacter actinomycetemcomitans leukotoxin is post-translationally modified by addition of either saturated or hydroxylated fatty acyl chains.	aggregatibacter actinomycetemcomitans, a common inhabitant of the human upper aerodigestive tract, produces a repeat in toxin (rtx), leukotoxin (ltxa). the ltxa is transcribed as a 114-kda inactive protoxin with activation being achieved by attachment of short chain fatty acyl groups to internal lysine residues. methyl esters of ltxa that were isolated from a. actinomycetemcomitans strains jp2 and hk1651 and subjected to gas chromatography/mass spectrometry contained palmitoyl (c16:0, 27-29%) an ...	2011	21729247
nitric oxide production by a murine macrophage cell line (raw264.7 cells) stimulated with aggregatibacter actinomycetemcomitans surface-associated material.	nitric oxide (no) may play a crucial role in the pathogenesis of periodontal disease and, hence, the aim of the present study was to test the hypothesis that aggregatibacter actinomycetemcomitans surface-associated material (sam) stimulates inducible nitric oxide synthase (inos) activity and no production by the murine macrophage cell line raw264.7. cells were stimulated with untreated or heat-treated a.┬áactinomycetemcomitans sam and with or without pre-treatment with l-n(6)-(1-iminoethyl)-lysi ...	2011	21736946
analysis of the peri-implant microbiota in 90 dental implants and its relationship to crevicular fluid volume.	objective: to evaluate the presence within the peri-implant sulcus of tannerela forsythia (tf), porphyromonas gingivales (pg), treponema denticola (td) and aggregatibacter actinomycetemcomitans (aa), and relate these bacteria to the peri-implant crevicular fluid volume (picfv). material and method: a prospective and cross-sectional clinical case series study was made. for the measurement of crevicular fluid, use was made of the periotron® 8000 (proflow incorporated. new york, usa), measuring the ...	2011	21743411
clinical and microbiological results 12 months after scaling and root planing with different irrigation solutions in patients with moderate chronic periodontitis ?a pilot randomized trial.	aim: the aim of this study was to determine in a randomized trial the impact on treatment outcome after 12 months of different subgingival irrigation solutions during scaling and root planing (srp). materials and methods: 51 adult volunteers with generalized chronic periodontitis were treated by full mouth srp using 0.9 % sodium chloride, 0.12 % chlorhexidine digluconate or 7.5 % povidone-iodine for subgingival irrigation during srp. prior to srp, after three and twelve months probing depth (pd) ...	2011	21749169
detection of periodontal markers in chronic periodontitis.	the aim was to compare the detection frequency of periodontopathogens by using the pado test 4.5 and checkerboard dna-dna hybridization technique in chronic periodontitis patients.thirty patients with chronic periodontitis were tested cross-sectionally with dna/rna oligogenomic probe method (iai pado test 4.5) and dna/dna whole genomic probe (checkerboard) method. samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the t ...	2011	21769304
effect of green tea catechin, a local drug delivery system as an adjunct to scaling and root planing in chronic periodontitis patients: a clinicomicrobiological study.	evaluate the adjunctive use of locally delivered green tea catechin with scaling and root planing, as compared to scaling and root planing alone in the management of chronic periodontitis.	2011	21772720
isolation of aggregatibacter aphrophilus from a patient with acute appendicitis.	acute appendicitis is a common surgical emergency. the etiology and pathophysiology of appendicitis have been well investigated. aggregatibacter aphrophilus is a fastidious gram-negative coccobacilli. detection of this organism in clinical samples and its differentiation from haemophilus aphrophilus or from aggregatibacter actinomycetemcomitans in routine microbiology settings could be difficult.	2011	21780555
bacterial leakage in implants with different implant-abutment connections: an in vitro study.	two-piece implants present gaps and cavities between the implant and the abutment, and these hollow spaces can act as a trap for bacteria. the aim of the present in vitro study was to evaluate the migration of two different microbial species from the inside to the outside of the implant-abutment assembly in three different connection types.	2012	21780904
prevotella nigrescens and porphyromonas gingivalis are associated with signs of carotid atherosclerosis in subjects with and without periodontitis.	yakob m, söder b, meurman jh, jogestrand t, nowak j, söder p.-ö. prevotella nigrescens and porphyromonas gingivalis are associated with signs of carotid atherosclerosis in subjects with and without periodontitis. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01398.x. © 2011 john wiley & sons a/s background and objective: oral microorganisms may be involved in the development of cardiovascular diseases, and porphyromonas gingivalis is one of the periodontal microorganisms that has been fou ...	2011	21793826
dose-response met-rantes treatment of experimental periodontitis: a narrow edge between the disease severity attenuation and infection control.	chemokines and chemokine receptors have been implicated in the selective migration of leukocyte subsets to periodontal tissues, which consequently influences the disease outcome. among these chemoattractants, the chemokines ccl3, ccl4 and ccl5 and its receptors, ccr1 and ccr5, have been associated with increased disease severity in mice and humans. therefore, in this study we investigated the modulation of experimental periodontitis outcome by the treatment with a specific antagonist of ccr1 and ...	2011	21799885
inhibitory effect of oral lactobacillus against oral pathogens.	aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ...	2011	21801186
antibacterial effect of silver-platinum coating for orthodontic appliances.	abstract objective: to develop a hard coating for stainless surfaces based on silver (ag)-platinum (pt) alloys. materials and methods: ag-pt alloys, which have high degree of biocompatibility, excellent resistance to sterilization conditions, and antibacterial properties to different bacteria, are associated with long-term antibacterial efficiency. approximately 1.03-µm to 2.34-µm-thick coatings, as determined by scanning electron microscopy, were deposited on stainless surfaces by the simultane ...	2011	21810004
identification of the pangenome and its components in 14 distinct aggregatibacter actinomycetemcomitans strains by comparative genomic analysis.	aggregatibacter actinomycetemcomitans is genetically heterogeneous and comprises distinct clonal lineages that may have different virulence potentials. however, limited information of the strain-to-strain genomic variations is available.	2011	21811606
aerosol deposition of hydroxyapatite and 4-hexylresorcinol coatings on titanium alloys for dental implants.	purpose: aerosol deposition is a newly developed technique, and it can deliver the drug from a hydroxyapatite (ha)-coated surface. 4-hexylresorcinol (4-hr) is a well-known antiseptic. the influence of the 4-hr component of ha coatings on titanium surfaces was studied in vitro and in vivo. materials and methods: x-ray diffraction and fourier transform infrared techniques were used for the evaluation of the coating. the cellular response of the coating was evaluated by scanning electron microscopi ...	2011	21821331
microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment.	abstract objective: to evaluate changes in subgingival microbiota before and during the leveling and alignment orthodontic stage using the polymerase chain reaction (pcr) method. materials and methods: thirty orthodontic patients (17 females and 13 males; aged 16.7 -¦ 6.5-áy) were included in this study. subgingival microbial samples were taken from the disto-buccal gingival crevice of the left upper central incisors, the left lower central incisors, the mesio-buccal gingival crevice of the left ...	2011	21827233
Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy.	BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ...	2011	21830010
in vitro evaluation of bacterial leakage along the implant-abutment interface of an external-hex implant after saliva incubation.	the aim of this in vitro study was to evaluate bacterial leakage along the implant-abutment interface under unloaded conditions. twelve premachined abutments with plastic sleeves and 12 dental implants were used in this study. prior to tests of bacterial leakage, samples from the inner parts of the implants were collected with sterile microbrushes to serve as negative controls for contamination. after casting, the abutments were tightened to 32 ncm on the implants. the assemblies were immersed i ...	2011	21841988
efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms.	objective: to determine in vitro the action of chlorhexidine digluconate and different commercially available mouthrinses on oral microorganisms. method and materials: minimal inhibitory concentrations and possible induction of resistance by chlorhexidine digluconate, an essential oil-containing mouthwash and an amine fluoride/stannous fluoride solution, were determined against microorganisms normally found in the oral cavity (10 streptococci, 2 enterobacteria, 1 candida albicans, 8 porphyromona ...	2011	21842009
Comparative evaluation of two structurally related flavonoids, isoliquiritigenin and liquiritigenin, for their oral infection therapeutic potential.	Isoliquiritigenin (1) and liquiritigenin (2) are structurally related flavonoids found in a variety of plants. The purpose of this study was to perform a comparative analysis of biological properties of these compounds in regard to their therapeutic potential for oral infections. Compound 1 demonstrated significant antibacterial activity against three major periodontopathogens, Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. In contrast, 2 exerted less pronounced ef ...	2011	21866899
proteomic and immunoproteomic analysis of aggregatibacter actinomycetemcomitans jp2 clone strain hk1651.	the proteome of aggregatibacter actinomycetemcomitans hk1651 (jp2 clone) and immunoreactive antigens were studied by two-dimensional (2d) gel electrophoresis, matrix-assisted laser desorption time-of-flight mass spectrometry (maldi-tof-ms), and 2d immunoblotting. the highly leukotoxic jp2 clone of a. actinomycetemcomitans is strongly associated with aggressive periodontitis (agp) in adolescents of north-west african descent and the pathogenicity of this bacterium is of major interest. hence, we ...	2011	21867783
A novel gene required for natural competence in Aggregatibacter actinomycetemcomitans.	Tanaka A, Fujise O, Chen C, Miura M, Hamachi T, Maeda K. A novel gene required for natural competence inAggregatibacter actinomycetemcomitans. J Periodont Res 2012; 47: 129-134. © 2011 John Wiley & Sons A/S Background and Objective: Natural competence is the ability of bacteria to take up extracellular DNA and incorporate it into their genomes. Some strains of Aggregatibacter actinomycetemcomitans, a critical periodontal pathogen, are naturally competent for transformation. However, information ...	2011	21883229
irsogladine maleate regulates epithelial barrier function in tumor necrosis factor-α-stimulated human gingival epithelial cells.	fujita t, yumoto h, shiba h, ouhara k, miyagawa t, nagahara t, matsuda s, kawaguchi h, matsuo t, murakami s, kurihara h. irsogladine maleate regulates epithelial barrier function in tumor necrosis factor-α-stimulated human gingival epithelial cells. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01404.x. ©2011 john wiley & sons a/s background and objective: as epithelial cells function as a mechanical barrier, the permeability of the gingival epithelial cell layer indicates a defensive cap ...	2011	21895660
Levels of periodontal pathogens in neonatal gastric aspirates and possible maternal sites of origin.	Maternal periodontal infection has been recognized as a risk factor for preterm and low birthweight infants. It is suspected that pathogens causing periodontal disease may translocate to the amniotic cavity and so contribute to triggering an adverse pregnancy outcome. This study aimed to determine levels and proportions of periodontal bacteria in neonatal gastric aspirates obtained from complicated pregnancies and the respective maternal oral and vaginal samples using a quantitative polymerase c ...	2011	21896155
microbiologic findings 1 year after partial- and full-mouth scaling in the treatment of moderate chronic periodontitis.	to investigate the impact on microbiologic variables of full-mouth scaling (fms) and conventional scaling and root planing (csrp) after 12 months.	2011	21909493
diode laser activated indocyanine green selectively kills bacteria.	commercially available photodynamic therapy for periodontal diseases utilizes methylene blue as a photosensitizer. here we propose a novel photosensitizer dye, indocyanine green (icg), because it can be readily activated by commercially available dental 810 nm diode lasers and has an established safety record as an intravascular agent in cardiac imaging and ophthalmologic photodynamic therapy. therefore, we aim to characterize icg uptake and killing of key periodontal pathogens to explore its po ...	2011	21913603
molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan.	periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ...	2011	21916371
RbsB (NTHI_0632) mediates quorum signal uptake in nontypeable Haemophilus influenzae strain 86-028NP.	Nontypeable Haemophilus influenzae (NTHI) is a respiratory commensal and opportunistic pathogen, which persists within biofilms on airway mucosal surfaces. For many species, biofilm formation is impacted by quorum signalling. Our prior work shows that production of autoinducer-2 (AI-2) promotes biofilm development and persistence for NTHI 86-028NP. NTHI 86-028NP encodes an ABC transporter annotated as a ribose transport system that includes a protein (RbsB) with similarity to the Escherichia col ...	2011	21923771
In vitro antibacterial activity and cytocompatibility of bismuth doped micro-arc oxidized titanium.	Chemical manipulations of the implant surface produce a bactericidal feature to prevent infections around dental implants. Despite the successful use of bismuth against mucosal and dermis infections, the antibacterial effect of bismuth in the oral cavity remains under investigation. The aim of this study was to evaluate the antibacterial activities of bismuth compounds against Actinobacillus actinomycetemcomitans, Staphylococcus mutans, and methicillin-resistant Staphylococcus aureus (MRSA), and ...	2011	21926149
colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers.	gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00506.x colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers objective: the purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. we also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria. materials and methods: forty edentulous and 37 dentate ...	2011	21929616
prevalence and antimicrobial susceptibility pattern of pathogens isolated from patients with juvenile periodontitis in jamaica: a prospective multi-centre study of 15 cases over a 15-year period.	prevalence and antimicrobial susceptibility pattern of most frequent pathogens isolated from patients treated with juvenile periodontitis at three separate dental centres in jamaica from 1989 to 2003 were studied. swabs were taken from these patients periodontal pathologic pocket or root of most of their teeth with active disease processes. these swabs were processed at the microbiology department of the university hospital of the west indies kingston, jamaica and the microbiology laboratory, sc ...	2011	21942137
salivary levels of antibacterial peptide (ll-37/hcap-18) and cotinine in periodontitis patients.	background: the purpose of this study was to examine the relationship between salivary ll-37 levels and clinical severity in chronic periodontitis (cp) patients. the presence/absence of four periodontopathic bacteria and salivary cotinine levels were also examined in order to assess the impact of these factors on ll-37 production. methods: unstimulated salivary samples were collected from 69 cp patients. salivary concentrations of ll-37 and cotinine were measured by enzyme-linked immunosorbent ...	2011	21942788
Antigen-presenting cell marker expression and phagocytotic activity in periodontal ligament cells.	J Oral Pathol Med (2011) Background: Periodontal ligament (PDL) cells are the main cellular constituents of the periodontium, maintain the integrity of the connective tissue, and impact pathology in periodontitis. The aim of this study was to analyze whether PDL cells recognize foreign particles and participate in the immune response to periodontal pathogens. Methods: Expression of surface proteins characteristic of antigen-presenting cells (APCs) (major histocompatibility complex [MHC] class ...	2011	21950503
Effects of low-dose doxycycline on cytokine secretion in human monocytes stimulated with Aggregatibacter actinomycetemcomitans.	Doxycycline is an antibiotic used in the treatment of a variety of inflammatory conditions, including periodontitis. Apart from its antimicrobial properties, this drug also has independent anti-inflammatory effects at sub-antimicrobial doses. The present study aimed to investigate the effects of low-doses of doxycycline (LDD) on cytokine production by human monocytic cells challenged with the periodontal pathogen Aggregatibacter actinomycetemcomitans, for up to 6 h. The simultaneous regulation o ...	2011	21962932
effect of a self-etching adhesive containing an antibacterial monomer on clinical periodontal parameters and subgingival microbiologic composition in orthodontic patients.	the aims of this study were to evaluate the effect of a self-etching adhesive system containing an antibacterial monomer on periodontal health and subgingival microbiologic composition in orthodontic patients and to compare it with a conventional adhesive system.	2011	21967952
Kingella kingae endocarditis: A rare case of mitral valve perforation.	Kingella kingae, a HACEK (Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, Cardiobacterium hominis, Eikenella corrodens, Kingella kingae) organism, is a common resident of the upper airway in children; it has been associated with endocarditis in children with pre-existing heart conditions. This case report describes K. kingae endocarditis leading to valvular damage in a previously healthy 18-month-old child. Our patient developed a K. kingae bactere ...	2011	21976892
the extended signal peptide of the trimeric autotransporter emaa of aggregatibacter actinomycetemcomitans modulates secretion.	the extracellular matrix protein adhesin a (emaa) of the gram-negative bacterium aggregatibacter actinomycetemcomitans is a fibrillar collagen adhesin belonging to the family of trimeric autotransporters. the protein forms antenna-like structures on the bacterial surface required for collagen adhesion. the 202-kda protein monomers are proposed to be targeted and translocated across the inner membrane by a long signal peptide composed of 56 amino acids. the predicted signal peptide was functional ...	2011	22001514
Aggregatibacter actinomycetemcomitans serotypes infections and periodontal conditions: a two-way assessment.	This study investigated a large population of individuals positive for A. actinomycetemcomitans and performed a two way analysis assessing the relation between the different serotypes of the bacterium and periodontal conditions. The serotypes analysis (serotypes a, b, c, d, e, f) showed that out of the 204 selected individuals positive for A. actinomycetemcomitans, 152 were positive for a single serotype, 27 showed a variable mixed infection and 25 individuals were not positive for any of the se ...	2011	22006011
the effect of spiramycin on porphyromonas gingivalis and other "classic" periopathogens.	in clinical trials, spiramycin has shown additional benefit overscaling and root planing on pocket depth reduction, but its effect on periodontal microbiota was evaluated only by darkfield microscopy. therefore, this study was conducted to determine the effect of spiramycin administration on porphyromonas gingivalis and other periodontopathic bacteria using 16s rarn pcr technique. thirty two non-smoker adults with untreated periodontitis and pocket depth > or = 7 mm. were evaluated to participat ...	2011	22010417
in vitro effect of photodynamic therapy on aggregatibacter actinomycetemcomitans and streptococcus sanguinis.	new periodontal disease treatments are needed to prevent infection progression. photodynamic therapy (pdt) is one of the greatest pledges for this purpose. it involves the use of light of specific wavelength to activate a nontoxic photosensitizing agent in the presence of oxygen for eradication of target cells, and can be used for photokilling of microorganisms. this study evaluated in vitro the photodynamic effect of 0.01% toluidine blue-o (tbo) in combination with an algainp diode laser light ...	2011	22011896
mif induces osteoclast differentiation and contributes to progression of periodontal disease in mice.	periodontal disease (pd) is a chronic inflammatory and alveolar bone destructive disease triggered by microorganisms from the oral biofilm. oral inoculation of mice with the periodontopathogen aggregatibacter actinomycetemcomitans (aa) induces marked alveolar bone loss and local production of inflammatory mediators, including macrophage migration inhibitory factor (mif). the role of mif for alveolar bone resorption during pd is not known. in the present study, experimental pd was induced in balb ...	2011	22016007
perinuclear localization of internalized outer membrane vesicles carrying active cytolethal distending toxin from aggregatibacter actinomycetemcomitans.	aggregatibacter actinomycetemcomitans is implicated in aggressive forms of periodontitis. similarly to several other gram-negative species, this organism produces and excretes a cytolethal distending toxin (cdt), a genotoxin associated with cell distention, g(2) cell cycle arrest, and/or apoptosis in many mammalian cell types. in this study, we have identified a. actinomycetemcomitans outer membrane vesicles (omvs) as a vehicle for simultaneous delivery of multiple proteins, including cdt, into ...	2012	22025516
the importance of the presence of aggregatibacter actinomycetemcomitans in sulcus gingivalis of patients with cardiovascular diseases.	over-replication of periodontal pathogens in the periodontium induces production of proinflammatory cytokines and c-reactive protein that can stimulate systemic inflammatory status and can initiate atherosclerosis and its consequences. in our pilot study we examined whether periodontal status and serum levels of interleukin-6 and c-reactive protein are associated with the presence of aggregatibacter actinomycetemcomitans in the periodontium of patients with cardiovascular diseases (cvd).	2011	22037744
clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole.	background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ...	2011	22060046
gangliosides block aggregatibacter actinomycetemcomitans leukotoxin (ltxa)-mediated hemolysis.	aggregatibacter actinomycetemcomitans is an oral pathogen and etiologic agent of localized aggressive periodontitis. the bacterium is also a cardiovascular pathogen causing infective endocarditis. a. actinomycetemcomitans produces leukotoxin (ltxa), an important virulence factor that targets white blood cells (wbcs) and plays a role in immune evasion during disease. the functional receptor for ltxa on wbcs is leukocyte function antigen-1 (lfa-1), a ß-2 integrin that is modified with n-linked car ...	2010	22069577
Aggregatibacter actinomycetemcomitans Leukotoxin: A Powerful Tool with Capacity to Cause Imbalance in the Host Inflammatory Response.	Aggregatibacter actinomycetemcomitans has been described as a member of the indigenous oral microbiota of humans, and is involved in the pathology of periodontitis and various non-oral infections. This bacterium selectively kills human leukocytes through expression of leukotoxin, a large pore-forming protein that belongs to the Repeat in Toxin (RTX) family. The specificity of the toxin is related to its prerequisite for a specific target cell receptor, LFA-1, which is solely expressed on leukocy ...	2011	22069708
inhibitory effect of quercetin on periodontal pathogens.	periodontal disease and associated bone loss can severely hinder orthodontic treatment. actinobacillus actinomycetemcomitans (aa) and porphyromonas gingivalis (pg) are bacteria which are strongly associated with this type of bone loss. quercetin, a unique flavonoid, which is found in many common foods including apples, onions and tea may have a effect against these bacteria.	2008	22073470
Associations of periodontal microorganisms with salivary proteins and MMP-8 in gingival crevicular fluid.	OBJECTIVE: We investigated in subjects with and without periodontitis, the levels of certain salivary proteins and matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF), in relation to the presence of specific periodontal pathogens. METHODS: Clinical parameters were recorded at baseline, in 1985 and in 2009 from 99 subjects; 56 with and 43 without periodontitis (mean age 59.2 ± SD 2.9). Saliva samples collected in 2009 were analysed for salivary albumin, total protein and immunog ...	2011	22103335
Aggressive periodontitis: how does an understanding of the pathogenesis affect treatment?	Aggressive periodontitis is a relatively rare periodontal condition that can result in significant attachment loss over a short period of time. As the disease is difficult to manage, owing to its rapid progression and severity, a variety of adjuncts have been advocated in its management. The authors outline concepts of the aetiology and pathogenesis of aggressive periodontitis and how the different treatment regimens relate to the current understanding of the disease process. Clinical Relevance: ...	2011	22128629
high frequency of tropheryma whipplei in culture-negative endocarditis.	"classical" whipple's disease (cwd) is caused by tropheryma whipplei and is characterized by arthropathy, weight loss, and diarrhea. t. whipplei infectious endocarditis (twie) is rarely reported, either in the context of cwd or as isolated twie without signs of systemic infection. the frequency of twie is unknown, and systematic studies are lacking. here, we performed an observational cohort study on the incidence of t. whipplei infection in explanted heart valves in two german university center ...	2011	22135251
the subgingival microbiota of papillon-lefèvre syndrome.	background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ...	2011	22141356
animal models to study host-bacteria interactions involved in periodontitis.	animal models have distinct advantages because they can mimic cellular complexities that occur in humans in vivo and are often more accurate than in vitro studies that take place on plastic surfaces with limited numbers of cell types present. furthermore, cause and effect relationships can be established by applying inhibitors or activators or through the use of genetically modified animals. such gain or loss of function studies are often difficult to achieve in human clinical studies, particula ...	2012	22142960
antimicrobial advances in treating periodontal diseases.	antibiotics are generally an efficient means of treating bacterial infections, and therefore are an obvious candidate in the treatment of periodontal diseases. systemically and locally administered antimicrobial agents of all kinds have been evaluated in multiple clinical trials. the vast majority of studies have tested antibiotics as adjuncts to non-surgical debridement. no regime has demonstrated superiority over systemically administered amoxicillin and metronidazole in the treatment of any c ...	2012	22142961
[Role of several periodontopathogenic microorganisms and tlr4 gene Asp299Gly polymorphism in atherosclerosis pathogenesis].	Establishment of presence of periodonto-pathogenic microorganisms in atherosclerosis plaque and surrounding tissues, and possible relation of development of atherosclerosis and TLR4 gene Asp299Gly polymorphism in ischemic heart disease patients (IHD).	2011	22145357
Correlation of the amino-acid sequence and the 3D structure of the functional domain of EmaA from Aggregatibacter actinomycetemcomitans.	Adhesion to collagen is an important virulence determinant for the periodontal pathogen Aggregatibacter actinomycetemcomitans. Binding to collagen is mediated by the extracellular-matrix protein adhesin-A (EmaA). EmaA is a homotrimeric autotransporter protein that forms flexible antenna-like appendages on the bacterium surface. An ellipsoidal structure at the distal end of the appendage, composed of three subdomains, contains the functional domain of the molecule. A correlation between amino-aci ...	2011	22155288
[enteric gram negative rods and unfermented of glucose bacteria in patients with peri-implant disease].	implants can be colonized by microorganisms from oral biofilms and may affect peri-implant tissues health. among these bacteria, pathogens typically associated with periodontitis can be found, such as aggregatibacter actinomycetemcomitans and porphyromonas gingivalis, as well as gram negative enteric bacilli not typically associated with periodontal diseases.	2011	22159480
ralstonia solanacearum needs flp pili for virulence on potato.	type iv pili are virulence factors in various bacteria. several subclasses of type iv pili have been described according to the characteristics of the structural prepilin subunit. although type iva pili have been implicated in the virulence of ralstonia solanacearum, type ivb pili have not previously been described in this plant pathogen. here, we report the characterization of two distinct tad loci in the r. solanacearum genome. the tad genes encode functions necessary for biogenesis of the ...	2011	22168446
Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis.	OBJECTIVE: The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR). MATERIALS AND METHODS: Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomit ...	2011	22169888
a five-species transcriptome array for oral mixed-biofilm studies.	oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs.	2011	22194794
evidence for graft colonization with periodontal pathogens in lung transplant recipients.	summary bronchiolitis obliterans syndrome (bos) is a major cause of late graft dysfunction in lung transplant recipients. there is increasing evidence that beside alloimmunologic injury also non-alloimmunologic inflammatory conditions may raise the risk of acute and chronic rejection. the oral cavity represents a possible reservoir for pathogenic bacteria due to its close anatomical proximity. in this pilot study, the presence of pathogenic periodontal bacteria in the oral cavity as well as in t ...	2011	22203528
implants with external hexagon and conical implant-abutment connections: an in vitro study of the bacterial contamination.	abstract prevention of microbial leakage at the implant-abutment junction is a major challenge for the construction of two-stage implants in order to minimize inflammatory reactions and to maximize bone stability at the implant neck. the aim of the present in vitro study was an evaluation of the leakage observed, over a period of 28 days, in cone morse taper internal connections and in screwed-abutments connections. in the present study ten specimens of cone morse (group 1) and ten of intern ...	2011	22208157
adhesion of aggregatibacter actinomycetemcomitans and fusobacterium nucleatum on bioactive tio(2) surfaces.	bioactive tio(2) coatings have been found to enhance fibroblast adhesion and gingival attachment on the titanium surfaces, but no information is available whether the coatings also promote the adhesion of periodontal pathogens.	2013	22220677
full-mouth disinfection and systemic antimicrobial therapy in generalized aggressive periodontitis: a randomized, placebo-controlled trial.	aim: the present investigation aimed to analyse clinical and microbiological effects of systemic administration of metronidazole and amoxicillin combined with the one-stage-full-mouth-disinfection protocol (osfmd) in generalized aggressive periodontitis patients (g-agp). materials and methods: thirty-nine systemically healthy patients with g-agp were consecutively included. the test group (n = 19) received amoxicillin-metronidazole combination (500 mg of each, three times a day for 7 days) and t ...	2012	22220822
investigation and quantification of key periodontal pathogens in patients with type 2 diabetes.	diabetes is a recognized risk factor for periodontitis. there are conflicting data regarding whether healthy diabetic patients or diabetic patients with chronic periodontitis have an altered subgingival microbiota compared with nondiabetic individuals. the aim of the present study was to detect quantitative differences in selected periodontopathogens in the subgingival plaque of diabetic patients using taqman quantitative pcr.	2012	22220967
release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment.	we aimed to achieve detailed biomaterials characterization of a drug delivery system for local periodontitis treatment based on electrospun metronidazole-loaded resorbable polylactide (pla) fibers.	2012	22226009
signaling transduction analysis in gingival epithelial cells after infection with aggregatibacter actinomycetemcomitans.	periodontal diseases result from the interaction of bacterial pathogens with the host's gingival tissue. gingival epithelial cells are constantly challenged by microbial cells and respond by altering their transcription profiles, inducing the production of inflammatory mediators. different transcription profiles are induced by oral bacteria and little is known about how the gingival epithelium responds after interaction with the periodontopathogenic organism aggregatibacter actinomycetemcomitans ...	2012	22230463
periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid.	in periodontitis, an effective host-response is primarily related to neutrophils loaded with serine proteases, including elastase (ne) and protease 3 (pr3), the extracellular activity of which is tightly controlled by endogenous inhibitors. in vitro these inhibitors are degraded by gingipains, cysteine proteases produced by porphyromonas gingivalis. the purpose of this study was to determine the level of selected protease inhibitors in gingival crevicular fluid (gcf) in relation to periodontal i ...	2012	22230465
the product of tadz, a new member of the para/mind superfamily, localizes to a pole in aggregatibacter actinomycetemcomitans.	aggregatibacter actinomycetemcomitans establishes a tenacious biofilm that is important for periodontal disease. the tad locus encodes the components for the secretion and biogenesis of flp pili, which are necessary for the biofilm to form. tadz is required, but its function has been elusive. we show that tadz genes belong to the para/mind superfamily of genes and that tadz from a. actinomycetemcomitans (aatadz) forms a polar focus in the cell independent of any other tad locus protein. mutation ...	2012	22239271
characterization of aggregatibacter actinomycetemcomitans strains in periodontitis patients in germany.	aggregatibacter actinomycetemcomitans strains of serotype b and with a deletion of 530 bp in the promoter region of the leukotoxin gene (jp2 clone) are known to be associated with severe periodontitis. our study was aimed to detect virulence genes of a. actinomycetemcomitans strains obtained from patients living in four german cities with different proportions of immigrants.	2012	22246112
the highly leukotoxic jp2 clone of aggregatibacter actinomycetemcomitans in localized and generalized forms of aggressive periodontitis.	abstract objective: to investigate the presence of a. actinomycetemcomitans, including the highly virulent jp2 clone, in young adult patients with aggressive periodontitis, and associate the findings with the two forms of the disease. materials and methods: seventy moroccan subjects with aggressive periodontitis, aged less than 35 years, were recruited. among these, 41 had lagp and 29 had gagp. plaque samples were collected from periodontal pockets and examined using a pcr that detects the pres ...	2012	22251014
microbial profile of patients with periodontitis compared with healthy subjects.	objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ...	2012	22257880
necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study.	objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ...	2012	22259811
[aggregatibacter actinomycetemcomitans].		2011	22286682
the combination of amoxicillin and metronidazole improves clinical and microbiologic results of one-stage, full-mouth, ultrasonic debridement in aggressive periodontitis treatment.	the aim of the present study is to assess clinical, microbiologic, and immunologic benefits of amoxicillin/metronidazole (am) when performing full-mouth ultrasonic debridement (fmud) in generalized aggressive periodontitis (gagp) treatment.	2012	22288485
no association between a actinomycetemcomitans or p gingivalis and chronic or aggressive periodontitis diagnosis.	only a few studies have attempted to detect differences in microbiologic profiles of patients with chronic periodontitis (cp) and aggressive periodontitis (agp). the aim of this analysis was to assess if clinical diagnosis or other subject factors showed association with the presence of actinobacillus actinomycetemcomitans and porphyromonas gingivalis in a cohort of periodontitis patients.	2012	22299125
detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction.	numerous reports have demonstrated that periodontal bacteria are present in plaques from atherosclerotic arteries. although periodontitis has recently been recognized as a risk factor for coronary artery disease, the direct relationship between periodontal bacteria and coronary artery disease has not yet been clarified. it has been suggested that these bacteria might contribute to inflammation and plaque instability. we assumed that if periodontal bacteria induce inflammation of plaque, the bact ...	2012	22305832
aggregatibacter actinomycetemcomitans leukotoxin cytotoxicity occurs through bilayer destabilization.	the gram-negative bacterium, aggregatibacter actinomycetemcomitans, is a common inhabitant of the human upper aerodigestive tract. the organism produces an rtx (repeats in toxin) toxin (ltxa) that kills human white blood cells. ltxa is believed to be a membrane-damaging toxin, but details of the cell surface interaction for this and several other rtx toxins have yet to be elucidated. initial morphological studies suggested that ltxa was bending the target cell membrane. because the ability of a ...	2012	22309134
antibacterial activity of the lactoperoxidase system combined with edible laminaria hot-water extract as a source of halide ions.	hot-water extracts prepared from nine out of 12 samples of dried edible laminaria reduced the viable numbers of aggregatibacter actinomycetemcomitans, staphylococcus aureus, and esherichia coli below the detection limit after incubation for 5 min when combined with lactoperoxidase, glucose oxidase, and glucose. some extracts showed higher bactericidal activity and a higher oi(-) concentration in the assay mixture after ultrafiltration.	2012	22313780
prevalence of periodontopathogens in a black brazilian secluded community matched with a black urban population.	to evaluate the prevalence of periodontopathogens according to periodontal profile in a black brazilian secluded community matched with an urban black population.	2011	22320070
azithromycin as an adjunctive treatment of generalized severe chronic periodontitis: clinical, microbiologic, and biochemical parameters.	this study examines the efficacy of azithromycin in combination with non-surgical periodontal therapy on clinical and microbiologic parameters and gingival crevicular fluid (gcf) matrix metalloproteinases-8 (mmp-8) levels over 6 months in patients with severe generalized chronic periodontitis (cp).	2012	22324488
rapid identification of oral isolates of aggregatibacter actinomycetemcomitans obtained from humans and primates by an ultrafast super convection based polymerase chain reaction.	aggregatibacter actinomycetemcomitans is a gram negative oral bacterium associated with localized aggressive periodontitis (lap). detection of a. actinomycetemcomitans in clinical samples is routinely done by pcr. our aim was to develop a rapid and reliable pcr method that can be used as a chair-side tool to detect a. actinomycetemcomitans in clinical samples. sensitivity and specificity assessment was performed on buccal and plaque samples obtained from 40 adolescents enrolled in an ongoing lap ...	2012	22326236
staphylococcus epidermidis biofilms: functional molecules, relation to virulence, and vaccine potential.	medical device-associated infections, most frequently caused by staphylococcus epidermidis and staphylococcus aureus, are of increasing importance in modern medicine. the formation of adherent, multilayered bacterial biofilms is crucial in the pathogenesis of these infections. polysaccharide intercellular adhesin (pia), a homoglycan of β-1,6-linked 2-acetamido-2-deoxy-d: -glucopyranosyl residues, of which about 15% are non-n-acetylated, is central to biofilm accumulation in staphylococci. it tra ...	2009	22328030
effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model.	oral bacterial biofilms are highly complex microbial communities with up to 700 different bacterial taxa. we report here the use of metatranscriptomic analysis to study patterns of community gene expression in a multispecies biofilm model composed of species found in healthy oral biofilms (actinomyces naeslundii, lactobacillus casei, streptococcus mitis, veillonella parvula, and fusobacterium nucleatum) and the same biofilm plus the periodontopathogens porphyromonas gingivalis and aggregatibacte ...	2012	22328675
genome sequence of aggregatibacter actinomycetemcomitans rhaa1, isolated from a rhesus macaque, an old world primate.	aggregatibacter actinomycetemcomitans is implicated in localized aggressive periodontitis. we report the first genome sequence of an a. actinomycetemcomitans strain isolated from an old world primate.	2012	22328766
in vitro efficacy of diallyl sulfides against the periodontopathogen aggregatibacter actinomycetemcomitans.	the in vitro antibacterial effects of diallyl sulfide (das) against the gram-negative periodontopathogen aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. a. actinomycetemcomitans was treated with garlic extract, allicin, or das, and the anti-a. actinomycetemcomitans effects of the treatment were evaluated. garlic extract, allicin, and das significantly inhibited the growth of a. act ...	2012	22330917
infection of human gingival fibroblasts with aggregatibacter actinomycetemcomitans: an in vitro study.	aggregatibacter actinomycetemcomitans is known to be a major cause of localized aggressive periodontitis. previous research has suggested that a. actinomycetemcomitans can damage many types of host cells. there is evidence for the ability of this organism to invade endothelial and epithelial cells, but information pertaining to its potential for invading gingival fibroblasts is very limited. internalization of bacteria is not only responsible for damaging host tissue but also a means to evade th ...	2012	22348592
[effect of periodontal surgery on the subgingival periodontal microbiota in artificial class iii furcation defects: an experimental study in monkeys].	this study was to longitudinally evaluate the change of prevalence of five periodontal putative pathogens in the subgingival plaque of artificial class iii furcation defects at the three time-points, including before the establishment of furcation defects, before and 6 months after periodontal surgery.	2012	22353894
[dissertations 25 years after date 31. black-pigmented oral bacteria in human oral infections].	in 1986, oral microbiology, and especially the microbiology of periodontal infections, was of central importance. interest in periodontology was growing rapidly and with it, interest in developments in microbiology and the immunology of infectious diseases of the periodontium. at national and international meetings discussions took place concerning the role of the newly discovered bacterial species actinobacillus actinomycetemcomitans and the role of antibiotics in the treatment of severe period ...	2012	22368841
oral microbiota in swiss adolescents.	the purpose of the study was to determine the prevalence of different oral microbes in gingival plaque samples and in samples from the dorsum of the tongue in a swiss adolescent population.	2013	22371122
presence of jp2 and non-jp2 genotypes of aggregatibacter actinomycetemcomitans and attachment loss in adolescents in ghana.	limited data are reported concerning the presence of a. actinomycetemcomitans and attachment loss (al) in sub-saharan countries. the authors investigate the carrier frequency of jp2 and non-jp2 genotypes of a. actinomycetemcomitans and the presence of al in ghanaian adolescents and evaluate socioeconomic conditions and oral hygiene practices.	2012	22376208
immunoregulatory gene polymorphisms in japanese women with preterm births and periodontitis.	many studies have reported an association between periodontal disease and preterm birth, although this remains controversial. cytokines and antibodies produced to give resistance to infection can enter the bloodstream and cause preterm labor. we analyzed maternal genetic polymorphisms in various immunoregulatory genes that could affect both preterm birth and periodontitis. a total of 1099 women referred to the department of obstetrics and gynecology, niigata university medical and dental hospita ...	2012	22382006
the association of aggregatibacter actinomycetemcomitans with preeclampsia in a subset of japanese pregnant women.	to determine whether periodontitis and three prominent members of the periodontal flora are associated with the development of preeclampsia (hypertension plus proteinuria) materials and methods: the samples were composed of 127 systemically healthy women. within 5 days after labour, clinical periodontal parameters and aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in subgingival plaque were evaluated. maternal serum igg antibody specific for each bacter ...	2012	22393563