Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
identification of reproduction-related genes and ssr-markers through expressed sequence tags analysis of a monsoon breeding carp rohu, labeo rohita (hamilton).labeo rohita (ham.) also called rohu is the most important freshwater aquaculture species on the indian sub continent. monsoon dependent breeding restricts its seed production beyond season indicating a strong genetic control about which very limited information is available. additionally, few genomic resources are publicly available for this species. here we sought to identify reproduction-relevant genes from normalized cdna libraries of the brain-pituitary-gonad-liver (bpgl-axis) tissues of ad ...201323583682
proteomic analysis of epithelioma papulosum cyprini cells infected with spring viremia of carp virus.spring viremia of carp (svc), caused by spring viremia of carp virus (svcv) is an important disease due to its drastic effects on carp fisheries in many countries. to better understand molecular responses to svcv infection, two dimensional electrophoresis (2-de) and maldi-tof/tof were performed to investigate altered proteins in epithelioma papulosum cyprini cells (epcs). differentially expressed proteins in mock-infected epcs and svcv-infected epcs were compared. a total of 54 differentially ex ...201323583725
does interspecies hybridization affect the host specificity of parasites in cyprinid fish?host specificity varies among parasite species. some parasites are strictly host-specific, others show a specificity for congeneric or non-congeneric phylogenetically related host species, whilst some others are non-specific (generalists). two cyprinids, cyprinus carpio and carassius gibelio, plus their respective hybrids were investigated for metazoan parasites. the aim of this study was to analyze whether interspecies hybridization affects host specificity. the different degrees of host specif ...201323587287
characterization and physical mapping of 18s and 5s ribosomal genes in indian major carps (pisces, cyprinidae).characterization of the major (18s) and minor (5s) ribosomal rna genes were carried out in three commercially important indian major carp (imc) species, viz. catla catla, labeo rohita and cirrhinus mrigala along with their physical localizations using dual colour fluorescence in situ hybridization. the diploid chromosome number in the above carps was confirmed to be 50 with inter-species karyo-morphological variations. the 18s rdna signals were observed on 3 pair of chromosomes in c. catla and l ...201323587674
spring viraemia of carp (svc) in the uk: the road to freedom.spring viraemia of carp (svc) is a disease of international importance that predominantly affects cyprinid fish and can cause significant mortality. in the united kingdom (uk), svc was first detected in 1977 with further cases occurring in fisheries, farms, wholesale and retail establishments throughout england and wales (but not scotland, where few cyprinid populations exist, nor northern ireland where svc has never been detected) over the subsequent 30 years. following a control and eradicatio ...201323597621
cryopreservation of common carp (cyprinus carpio l.) sperm induces protein phosphorylation in tyrosine and threonine residues.the effect of cryopreservation on the protein phosphorylation/dephosphorylation pattern of common carp (cyprinus carpio) sperm is described. sperm was diluted in dimethyl sulfoxide (dmso) and ethylene glycol (eg)-based extenders, followed by equilibration, freezing, and thawing. proteins extracted from fresh and cryopreserved spermatozoa were separated on sds-page and two-dimensional gel electrophoresis, blotted on polyvinylidene difluoride membrane, and treated with anti-phosphotyrosine, anti-p ...201323602083
effects of atrazine and chlorpyrifos on the production of nitric oxide and expression of inducible nitric oxide synthase in the brain of common carp (cyprinus carpio l.).the study aimed to investigate the effects of atrazine (atr), chlorpyrifos (cpf), and the mixture of them on nitric oxide (no) and inducible nitric oxide synthase (inos) in the brain of common carp. the triazine herbicide atr and the organophosphorus insecticide cpf are frequently and extensively applied in agriculture all over the world. 220 carps were averagely divided into eleven groups according to the different treatments and concentration, including the exposure and recovery experiments. i ...201323602412
pouchitis: what every gastroenterologist needs to know.pouchitis is the most common complication among patients with ulcerative colitis who have undergone restorative proctocolectomy with ileal pouch-anal anastomosis. pouchitis is actually a spectrum of diseases that vary in etiology, pathogenesis, phenotype, and clinical course. although initial acute episodes typically respond to antibiotic therapy, patients can become dependent on antibiotics or develop refractory disease. many factors contribute to the course of refractory pouchitis, such as the ...201323602818
the complete mitochondrial genome of the japanese ornamental koi carp (cyprinus carpio) and its implication for the history of koi.complete mitochondrial genome (mitogenome) sequences were determined for two individuals of japanese ornamental koi carp. interestingly, the obtained mitogenomes (16,581 bp) were both completely identical to the recently reported mitogenome of oujiang color carp from china. control region (cr) sequences in dna database demonstrated that more than half (65%) of the koi carp individuals so far reported had partial or complete cr sequences identical to those from oujiang color carp. these results m ...201423607478
renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (carassius auratus).an acute toxicological experiment was designed to characterize the sequence of renal ultrastructural changes with accumulated mcs in crucian carp injected intraperitoneally (i.p.) with extracted microcystins (mainly mc-rr and -lr) at two doses, 50 and 200 μg mc-lreq. kg⁻¹ body weight. quantitative and qualitative determinations of mcs in the kidney were conducted by hplc and lc-ms, respectively. mc-rr content in kidney of crucian carp showed a time dose-dependent increase within 48 h post-inject ...201323608020
[evaluation of ten fish species to be included as part of renal diet, due to their protein, phosphorus and fatty acids content].because renal disease is highly complex, its nutritional treatment is complicated and many foods are restricted, including fish because its phosphorus content. the aim of the present study was to analyze ten fillet fish species, commonly consumed in mexico (cyprinus carpio carpio, ophichthus rex, symphurus elongatus, eucinostomus entomelas, chirostoma patzcuaro, bairdiella chrysoura, salmo salar oreochromis urolepis hornorum, sphyraena guachancho, istiophorus albicans), to determine their phosph ...201223610899
relationship between brain and liver oxidative state and maximum lifespan potential of different fish species.the aim of the present study is to determine whether a relationship exists between the maximum lifespan potential (mlsp) of different fish species (squalius pyrenaicus, cyprinus carpio, carassius auratus, luciobarbus sclateri and oncorhynchus mykiss) and the oxidative state of brain and liver. for this purpose, the activity of the antioxidant enzymes superoxide dismutase (sod), catalase, glutathione peroxidase (gpx), glutathione reductase (gr), glutathione s-transferase (gst) and dt-diaphorase ( ...201323618756
smad3 mediates cigarette smoke extract (cse) induction of vegf release by human fetal lung fibroblasts.cigarette smoke is the major cause of chronic obstructive pulmonary disease (copd), yet pathogenic mechanisms are not fully understood. vascular endothelial growth factor (vegf) is one of the major regulators of endothelial cell survival and is believed to play a role in the pathogenesis of copd. fibroblasts are a significant source of vegf in the lungs; however the effect of cigarette smoke exposure on vegf release by fibroblasts is not fully understood. we hypothesized that cigarette smoke-ind ...201323618901
effect of ambient temperature increase on intestinal mucosa amylolytic activity in freshwater fish.the relationship between the level of the intestinal mucosa amylolytic activity (aa) of juvenile goldfish carassius auratus, carp cyprinus carpio, roach rutilus rutilus and perch perca fluviatilis and the rate of water temperature increase was studied. increase in water heating rate significantly influences aa and the level of upper lethal temperatures defined by critical thermal maximum (ctm) during different seasons. at a low rate of water temperature increase, 0.04 °c/h, aa and the level of c ...201323619779
novel seadornavirus (family reoviridae) related to banna virus in europe.banna virus, whose genome is composed of 12 segments of double-stranded rna, is a member of the genus seadornavirus in the family reoviridae and is thought to be an emerging mosquito-transmitted human pathogen in southeast asia. a novel phylogenetic relative of banna virus (balaton virus, balv, jx947843-jx947850 and kc522611-kc522612) was identified using viral metagenomics in the intestinal contents of freshwater carp (cyprinus carpio) in hungary. the amino acid sequence identity of balaton vir ...201323624680
transcriptome analysis of crucian carp (carassius auratus), an important aquaculture and hypoxia-tolerant species.the crucian carp is an important aquaculture species and a potential model to study genome evolution and physiological adaptation. however, so far the genomics and transcriptomics data available for this species are still scarce. we performed de novo transcriptome sequencing of four cdna libraries representing brain, muscle, liver and kidney tissues respectively, each with six specimens. the removal of low quality reads resulted in 2.62 million raw reads, which were assembled as 127,711 unigenes ...201323630630
effect of subacute exposure to silver nanoparticle on some hematological and plasma biochemical indices in silver carp (hypophthalmichthys molitrix).the use of silver nanoparticles (ag-nps) is rapidly increasing, but there are limited data on their effects on the aquatic environment. the present study aimed to determine the acute toxicity and evaluate the effect of subacute concentrations of ag-nps (nanocid®: average particle size of 61 nm) on hematological and plasma biochemical indices of silver carp, hypophthalmichthys molitrix, after 3, 7 and 14 days. the 24-, 48-, 72- and 96-h median lethal concentration (lc50) values of nanocid for sil ...201323632006
comparison of the lipid properties of healthy and pansteatitis-affected african sharptooth catfish, clarias gariepinus (burchell), and the role of diet in pansteatitis outbreaks in the olifants river in the kruger national park, south africa.pansteatitis has been identified in wild populations of sharptooth catfish, clarias gariepinus (burchell), and nile crocodiles, crocodylus niloticus laurenti, inhabiting the same waters in the olifants river gorge in the kruger national park, south africa. mesenteric and pectoral fat tissue was investigated microscopically and by fatty acid analysis in healthy and pansteatitis-affected catfish from both captive and wild populations. variation in fatty acid composition between pectoral and mesent ...201323634747
apoptosis in thymus of teleost fish.the presence and distribution of apoptotic cells during thymus development and in adult were studied by in situ end-labelling of fragmented dna in three temperate species carp (cyprinus carpio), sea bass (dicentrarchus labrax) and dusky grouper (epinephelus marginatus) and in the adult thymus of three antarctic species belonging to the genus trematomus spp. during thymus development some few isolated apoptotic cell (ac) firstly appeared in the central-external part of the organ (carp: 5 days ph; ...201323639932
molecular barcoding of an atypical cyprinid population assessed by cytochrome b gene sequencing.a fish population of the carp family cyprinidae with atypical phenotypic characteristics was observed in one of the main catchments of the pollino national park, a valuable, protected area in southern italy. in this area, the italian roach rutilus rubilio (bonaparte, 1837), a native endemic fish of tyrrhenean regions, has been introduced in sympatric conditions with squalius squalus (bonaparte, 1837) and telestes muticellus (bonaparte, 1837). a molecular investigation was carried out to assess t ...201323646946
the effects of caudal fin loss and regeneration on the swimming performance of three cyprinid fish species with different swimming capacities.in nature, the caudal fins of fish species are frequently lost to some extent by aggressive behaviour, predation and diseases. to test whether the swimming performance of fish with different swimming capacities would be differentially affected due to caudal fin loss and regeneration, we investigated the critical swimming speed (ucrit), swimming metabolic rate (m(o2)), tail beat frequency (f(tb)) and tail beat amplitude (a(tb)) after caudal fin loss and regeneration (20 days) in juveniles of thre ...201323661776
modulation of rh glycoproteins, ammonia excretion and na+ fluxes in three freshwater teleosts when exposed chronically to high environmental ammonia.we investigated relationships among branchial unidirectional na(+) fluxes, ammonia excretion, urea excretion, plasma ammonia, plasma cortisol, and gill transporter expression and function in three freshwater fish differing in their sensitivity to high environmental ammonia (hea). the highly ammonia-sensitive salmonid oncorhynchus mykiss (rainbow trout), the less ammonia-sensitive cyprinid cyprinus carpio (common carp) and the highly ammonia-resistant cyprinid carassius auratus (goldfish) were ex ...201323661781
two δ6-desaturase-like genes in common carp (cyprinus carpio var. jian): structure characterization, mrna expression, temperature and nutritional regulation.δ6-desaturase is the rate-limiting enzyme involved in highly unsaturated fatty acid (hufa) biosynthesis. there is very little information on the evolution and functional characterization of δ6fad-a and δ6fad-b in common carp (cyprinus carpio var. jian). in the present study, the genomic sequences and structures of two putative δ6-desaturase-like genes in common carp genome were obtained. we investigated the mrna expression patterns of δ6fad-a and δ6fad-b in tissue, hatching carp embryos, larvae ...201323664980
zinc transferrin stimulates red blood cell formation in the head kidney of common carp (cyprinus carpio).the common carp is one of the few fish able to tolerate extremely low oxygen levels. these fish store zinc in their digestive tract tissue and head kidney at concentrations of 300-500μg/g of fresh tissue, which is 5-10 times higher than in other fish. previous studies have indicated a link between the high zinc levels in the common carp and stress erythropoiesis. in this report, using suspension-cultured common carp head kidney cells with or without zncl2 supplementation, we found that zinc stim ...201323665074
diasteroisomer and enantiomer-specific profiles of hexabromocyclododecane and tetrabromobisphenol a in an aquatic environment in a highly industrialized area, south china: vertical profile, phase partition, and bioaccumulation.tetrabromobisphenol a (tbbpa) and hexabromocyclododecanes (hbcds) were determined in water, sediments, sediment cores, and three fish species from a river running through a highly industrialized area in south china. tbbpa concentrations exceeded those of hbcds in the sediment and the dissolved phase of water and its levels in fish were at the high end of worldwide figures. 26% of hbcds and 99% of tbbpa were found in dissolved phase of water. plecostomus occupying high trophic level exhibited hig ...201323665846
supplementation of microbial levan in the diet of cyprinus carpio fry (linnaeus, 1758) exposed to sublethal toxicity of fipronil: effect on growth and metabolic responses.a 60-day feeding trial was conducted to study the effect of dietary microbial levan on growth performance and metabolic responses of cyprinus carpio fry exposed to sublethal dose (1/10th lc₅₀) of fipronil [(±)-5-amino-1-(2,6-dichloro-α,α,α-trifluoro-p-tolyl)-4-trifluoromethylsulfinylpyrazole-3-carbonitrile]. two hundred and twenty five fry were randomly distributed in five treatments in triplicates. four purified diets were prepared with graded levels of microbial levan. five different treatment ...201323666372
molecular characterization of sphaerospora molnari (myxozoa), the agent of gill sphaerosporosis in common carp cyprinus carpio carpio.sphaerospora molnari lom, dyková, pavlásková and grupcheva, 1983 often causes severe infections in the gills and skin of common carp fingerlings cyprinus carpio carpio in central europe. although most sphaerospora spp. are coelozoic and affect the excretory system of fish, s. molnari develops mature spores in the epithelia of gill filaments, making it a rare representative of histozoic freshwater species within the genus. on the basis of a partial 18s rdna sequence assigned as belonging to s. mo ...201323670080
effects of dietary β-(1,3)(1,6)-d-glucan supplementation on growth performance, intestinal morphology and haemato-immunological profile of mirror carp (cyprinus carpio l.).in recent years, aquaculture research has focused on probiotics, prebiotics, and β-glucans, in order to improve health status and growth performance. information regarding the effects of β-glucan on growth performance and intestinal immunity of mirror carp (cyprinus carpio l.) is scarce. an experiment was therefore conducted to investigate the effects of a yeast β-glucan preparation (macrogard(®) ) on growth performance, intestinal morphology and haemato-immunological indices of mirror carp. car ...201423676107
variations in the microcystin content of different fish species collected from a eutrophic lake.microcystins produced from cyanobacteria can accumulate in fish tissues. liquid chromatography coupled with tandem quadrupole mass spectrometry (lc-ms/ms) is an attractive alternative to immunoassays for the determination of low concentrations of microcystins in tissues. fish taken from grand lake st. marys, a eutrophic lake in ohio, usa, were analyzed for microcystin-lr in their fillets using lc-ms/ms. of 129 fish tested for microcystins, only black crappie (pomoxis nigromaculatus) and common c ...201323676698
bioaccumulation and neurotoxicity of dithiopyridine herbicide in the brain of freshwater fish, cyprinus carpio.the freshwater carp, cyprinus carpio, was exposed to 0.5 mg (30% of median lethal concentration (lc50)), 1.0 mg (60% of lc50), and 1.6 mg (lc50) of dithiopyridine herbicide per liter for acute (24 h) and 1/10 of lc50 (0.2 mg/l/day) for sublethal (1, 3, 7, 14, and 21 days) experiments. the herbicide bioaccumulation was significantly affected by the acute exposure levels and the experimental periods and was positively correlated with them. one-way analysis of variance revealed that the acute and s ...201523681548
evaluation of the effects of tricaine methanesulfonate on retinal structure and function in koi carp (cyprinus carpio).to determine whether repeated exposure to clinically relevant concentrations of tricaine methanesulfonate (ms-222) would alter retinal function or induce histologically detectable retinal lesions in koi carp (cyprinus carpio).201323683025
cloning of cytochrome p450 3a137 complementary dna in silver carp and expression induction by ionic liquid.cytochrome p450 (cyp) enzymes, especially cyp 3a, are responsible for metabolizing of various kinds of endogenous and exogenous compounds in animals. in the present study, a full-length sequence of cyp 3a137 cdna in silver carp was cloned and sequenced, and then a phylogenetic tree of cyp 3a was structured. additionally, the acute toxicity of the ionic liquid 1-octyl-3-methylimidazolium bromide ([c8mim]br) on silver carp and transcription and microsome enzyme activity of cyp 3a137 in the liver o ...201323683867
study the seasonal steroid hormones of common carp in caspian sea, iran.in this investigation, serum steroid hormones such as testosterone (t), 17β-estradiol (e2) and progesterone (p) in 12 female of the migratory population of common carp (cyprinus carpio) in southeast of caspian sea during a year from may 2011 to may 2012 were studied. the results of present study revealed that changes in levels of steroid hormones, (e2) and (t) were closely correlated to ovarian development. there was significant difference in level of 17 β- estradiol between autumn and winter se ...201323687630
determination of pentachlorophenol residue in meat and fish by gas chromatography-electron capture detection and gas chromatography-mass spectrometry with accelerated solvent extraction.a novel analytical method, using gas chromatography-electron capture detection (gc-ecd) and gc-mass spectrometry detection (ms), was developed for the qualitative and quantitative measurement of pentachlorophenol in meat and fish. the analyte was extracted by methanol-2% trichloroacetic acid (3/1, v/v) with accelerated solvent extraction (ase). the eluted fraction was evaporated and derivatized with acetic anhydride-pyridine (1/1, v/v) for gc-ecd analysis and gc-ms confirmation. the parameters f ...201423690067
recovery ability of common carp (cyprinus carpio) after a short-term exposure to terbuthylazine.effects of a high terbuthylazine concentration (3.3 mg/1) on cyprinus carpio were studied using a commercial herbicide formulation click 500 sc (terbuthylazine 500 g/l). the fish were exposed to the pesticide for 24 h and allowed to recover for 6 days. biometric parameters, plasma biochemical parameters and biomarkers of oxidative stress as well as histopathological changes in selected tissues were assessed on day 1 and 7. after a 24-h exposure, there were significant alterations found in the ac ...201323691571
biological response of high-back crucian carp (carassius auratus) during different life stages to wastewater treatment plant effluent.this study presents the adverse effects of endocrine disrupting chemicals (edcs) in effluent of wastewater treatment plants (wwtps) on fish health. a study of chronic exposure to wwtps effluent for 10 months was undertaken in high-back crucian carp (carassius auratus) during different life stages, covering early-life-stage (els), prespawning period, and postspawning period. condition factor (cf), gonadosomatic index (gsi), hepatosomatic index (hsi), and plasma vitellogenin (vtg) levels were empl ...201323695857
effect of atrazine and chlorpyrifos exposure on cytochrome p450 contents and enzyme activities in common carp gills.chlorpyrifos (cpf) and atrazine (atr) are the most widely used organophosphate insecticides and triazine herbicides, respectively, worldwide. this study aimed at investigating the effects of atr, cpf and mixture on common carp gills following 40-d exposure and 40-d recovery experiments. cytochrome p450 content, activities of aminopyrine n-demethylase (apnd) and erythromycin n-demethylase (ernd) and the mrna levels of the cyp1 family (cyp1a, cyp1b, and cyp1c) were determined. in total, 220 common ...201323702303
immunomodulatory effect of cimetidine in common carp (cyprinus carpio l.).the main indication of cimetidine is being h₂-receptor antagonist, but studies suggest that cimetidine may also act as a non-specific stimulant of cell-mediated immunity and immunomodulator. in order to determine the immunomodulatory effect of dietary intake of cimetidine in the common carp (100 ± 10 g), subjects were fed diets containing 0 (control), 50, 100 and 200 mg cimetidine kg⁻¹ of dry diet for a period of 6 weeks. tlc and nbt assays were significantly (p < 0.05) stimulated in cimetidine- ...201323703690
a dense genetic linkage map for common carp and its integration with a bac-based physical map.common carp (cyprinus carpio) is one of the most important aquaculture species with an annual global production of 3.4 million metric tons. it is also an important ornamental species as well as an important model species for aquaculture research. to improve the economically important traits of this fish, a number of genomic resources and genetic tools have been developed, including several genetic maps and a bacterial artificial chromosome (bac)-based physical map. however, integrated genetic an ...201323704958
the co-effect of organic matrix from carp otolith and microenvironment on calcium carbonate mineralization.in vitro mineralization experiment is an effective way to study the effect of organic matrix on calcium carbonate crystallization, and to reveal the relationship between organic matrix and inorganic crystal in natural biominerals. in natural biominerals, organic matrix plays an important role in crystal formation and stability, together with microenvironment changes, they can affect crystal polymorph, morphology, density, size, orientation etc. in this work, we systematically studied the effects ...201323706232
khawia abbottinae sp. n. (cestoda: caryophyllidea) from the chinese false gudgeon abbottina rivularis (cyprinidae: gobioninae) in china: morphological and molecular data.khawia abbottinae sp. n. is described from the chinese false gudgeon, abbottina rivularis (basilewsky) (cyprinidae: gobioninae), from the yangtze river basin in china. the new species can be distinguished from the congeneric species mainly by the arrangements of the testes, which form two longitudinal bands (other congeneric species have the testes irregularly scattered throughout the testicular region) and their number (at maximum 85 testes versus at least 160 in the other khawia spp.), and the ...201323724733
metacercariae of clinostomum schizothoraxi kaw, 1950 (digenea: clinostomatidae) in carassius carassius (linnaeus) under different environmental conditions.this report addresses the possible impacts of local habitat characteristics on the metacercariae of clinostomum schizothoraxi kaw, 1950 infecting crucian carp, carassius carassius (linnaeus), from three lakes in kashmir. the lakes chosen encompass an extremely wide gradient in trophic status--manasbal (less polluted and mesotrophic), dal (moderately polluted and eutrophic) and anchar (strongly polluted and hypertrophic). the results indicate that infrapopulation-level descriptors of abundance of ...201323724736
an evaluation of soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm.plant-derived lecithin has been used as a more sanitary alternative to avian egg yolk in livestock sperm cryopreservation protocols but its efficacy for cryopreserving fish sperm has not previously been tested comparatively. here various concentrations of soybean lecithin were evaluated for the cryopreservation of carp (cyprinus carpio) sperm. sexually mature fish were induced to spermiation and ovulation with ovopel. the extenders were prepared by using 300 mm glucose, 10% dmso, supplemented wi ...201323727066
toxic effects, bioconcentration and depuration of verapamil in the early life stages of common carp (cyprinus carpio l.).verapamil is a pharmaceutical that belongs to a group of calcium channel blockers and is mainly used as a treatment of angina pectoris and arterial hypertension. verapamil has been detected in aquatic environments in concentrations ranging from ng l(-1) to μg l(-1). in the present study, a series of acute toxicity tests of verapamil on various developmental stages of common carp (cyprinus carpio) were conducted. as a result, 96hlc50 values of verapamil were estimated at 16.4±9.2, 7.3±1.5 and 4.8 ...201323727993
residual levels, tissue distribution and risk assessment of organochlorine pesticides (ocps) in edible fishes from taihu lake, china.tissue distribution and bioaccumulation of organochlorine pesticides (ocps) in edible fishes collected from taihu lake, cyprinus carpio (c. carpio) and ctenopharyngodon idellus (c. idellus), were studied. ocps were detected in all samples with hexachlorocyclohexanes (hchs), aldrins (including aldrin, dieldrin, endrin, endrin aldehyde, and endrin ketone), heptachlors (heptachlor and heptachlor epoxide) and dichlorodiphenyltrichloroethanes (ddts) being the predominant compounds for both fish speci ...201323729160
characterization of a novel cell line from the caudal fin of koi carp cyprinus carpio.a continuous cell line (kf-101) derived from the caudal fin of the koi carp cyprinus carpio was established and characterized. the kf-101 cell line multiplied abundantly in leibovitz's l-15 medium containing 10% foetal bovine serum at 25° c, and was subcultured for >90 passages over a period of 3 years. immunocytochemistry revealed that the kf-101 cells contain keratin, junction proteins connexin-43 and occludin, and ectodermal stem-cell marker pax-6, but not vimentin. furthermore, the kf-101 ce ...201323731143
[sequences analysis of jlfabp2 and the correlation between polymorphisms and body weight gain in cyprinus carpio var. jian].two replicate intestine fatty acid binding protein genes (jlfabp2a and jlfabp2b) were cloned from cyprinus carpio var. jian using pcr. both orfs were 399 bp in length sharing 92.2% similarity with each other, and 88.0% and 90.5% with their counterpart in zebrafish, respectively. the gene structure of jlfabp2s was same as other fabps, which contained four exons and three introns. sequences and lengths of introns between 2a and 2b. were obviously different phylogenetic tree displayed that two jlfa ...201323732670
bioconcentration of perfluorinated alkyl acids: how important is specific binding?perfluorinated alkyl acids (pfaas) are important global pollutants with unique pharmacokinetics. evidence is accumulating that their behavior within organisms is affected by interaction with a number of proteins. in mammals, serum albumin, fatty acid binding proteins (fabps) and organic anion transporters (oats) have been identified as important to the tissue distribution, species-specific accumulation, and species- and gender-specific elimination rates of perfluoroalkyl carboxylates and perfluo ...201323734664
effects of deltamethrin on biometric parameters and liver biomarkers in common carp (cyprinus carpio l.).the present study is aimed to evaluate the effect of sublethal exposure to the pyrethroid pesticide deltamethrin, according to biometric parameters and important liver biomarkers in the common carp (cyprinus carpio). fish were exposed for 4, 15 and 30 days to deltamethrin mixed to the aquaria water at the concentrations of 0.08, 0.4 and 0.8μg/l. the exposure did not affect fish biometric parameters. glutathione-s-transferase (gst) activity in liver (hepatopancreas) increased in fish exposed to t ...201323736096
gut microbiota contributes to the growth of fast-growing transgenic common carp (cyprinus carpio l.).gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. to gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (cyprinus carpio l.) to study the connections between its large body feature and gut microbes. metagenome-based fingerprinting and high-throughput sequencing on bacterial 16s rrna genes indicated that fish gut wa ...201323741344
molecular characterization and expression analysis of tlr 7 and tlr 8 homologs in large yellow croaker (pseudosciaena crocea).the two toll-like receptor (tlr) genes, lyctlr7 and lyctlr8, were cloned from large yellow croaker (pseudosciaena crocea), an economically important marine fish in china. the full-length cdnas of lyctlr7 and lyctlr8 are 3544 and 3593 bp, with an open reading frame (orf) of 3165 and 3093 bp, encoding 1053 and 1030 amino acids, respectively. the tlr family motifs, such as leucine rich repeat (lrr) and toll/interleukin (il)-1 receptor (tir) domain, are conserved in the lyctlr7 and lyctlr8, with 17 ...201323742866
effects of dietary isoleucine on the immune response, antioxidant status and gene expression in the head kidney of juvenile jian carp (cyprinus carpio var. jian).this study was conducted to evaluate the effects of dietary isoleucine (ile) on the immune response, antioxidant status and gene expression in the head kidney of juvenile jian carp (cyprinus carpio var. jian). six semi-purified isonitrogenous diets (4.2, 7.0, 9.5, 11.9, 13.9 and 16.9 g ile kg(-1) diet) were fed to jian carp (6.9 ± 0.03 g) for 60 days. the results showed that ile supplementation improved the head kidney index, red and white blood cell counts, anti-hydroxyl radical capacity and th ...201323742869
effects of growth hormone (gh) transgene and nutrition on growth and bone development in common carp.limited information is available on effects of growth hormone transgene and nutrition on growth and development of aquatic animals. here, we present a study to test these effects with growth-enhanced transgenic common carp under two nutritional conditions or feeding rations (i.e., 5% and 10% of fish body weight per day). compared with the nontransgenic fish, the growth rates of the transgenic fish increased significantly in both feeding rations. the shape of the pharyngeal bone was similar among ...201323744555
cytocomposition of the vitellarium in khawia sinensis hsü, 1935 (cestoda, caryophyllidea, lytocestidae): another caryophyllidean species with lamellar bodies and lipids.the vitellarium of the invasive caryophyllidean tapeworm khawia sinensis hsü, 1935 from carp cyprinus carpio l. was examined by means of transmission electron microscopy and cytochemical staining for glycogen with periodic acid-thiosemicarbazide-silver proteinate (pa-tsc-sp). a vitellarium consists of numerous follicles of irregular size that are interconnected by a net of vitelline ducts. vitelline follicles are composed of vitelline cells at various stages of development that are interconnecte ...201323749090
high affinity of cadmium and copper to head kidney of common carp (cyprinus carpio l.).the aim of the present study was to evaluate bioaccumulation of metals in various tissues of the freshwater fish cyprinus carpio l. exposed to cadmium and copper (a xenobiotic and a microelement). the fish were subjected to short-term (3 h, cd-s and cu-s) or long-term (4 weeks, cd-l and cu-l) exposures to 100 % 96hlc₅₀ or 10 % 96hlc₅₀, respectively. blood, gill, liver, head and trunk kidney were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term ...201423756826
evidence for two koi herpesvirus (khv) genotypes in south korea.the geographic distribution of koi herpesvirus (khv) has recently been analyzed by polymerase chain reaction (pcr, based on the alleles of 3 domains) and sequence analysis using 3 regions of khv genomic dna (sphi-5, 9/5, and the thymidine kinase gene). in this study, samples from 6 carp showing symptoms of khv infection in 2008 were examined for the presence of khv by using pcr and cell culture isolation methods. khv was detected in 2 (pyeongtaek and buan) of the samples. sequence analysis revea ...201323759557
effects of fluoride on growth, body composition, and serum biochemical profile in a freshwater teleost, cyprinus carpio.fluoride is ubiquitously distributed in natural waters. elevated fluoride may cause histopathological changes and induce oxidative stress in the gills of the common carp (cyprinus carpio). the present study further evaluates the effects of fluoride on growth performance, body composition, and biochemical measurements of c. carpio. the results showed that food intake, growth, serum osmolality, body composition, and biochemical measures in the blood were affected by fluoride. weight gain rate and ...201323761139
transgenic common carp do not have the ability to expand populations.the ecological safety of transgenic organisms is an important issue of international public and political concern. the assessment of ecological risks is also crucial for realizing the beneficial industrial application of transgenic organisms. in this study, reproduction of common carp (cyprinus carpio, cc) in isolated natural aquatic environments was analyzed. using the method of paternity testing, a comparative analysis was conducted on the structure of an offspring population of "all-fish" gro ...201323762383
cell cycle and apoptosis regulatory protein (carp)-1 is expressed in osteoblasts and regulated by pth.bone mass is dependent on osteoblast proliferation, differentiation and life-span of osteoblasts. parathyroid hormone (pth) controls osteoblast cell cycle regulatory proteins and suppresses mature osteoblasts apoptosis. intermittent administration of pth increases bone mass but the mechanism of action are complex and incompletely understood. cell cycle and apoptosis regulatory protein (carp)-1 (aka ccar1) is a novel transducer of signaling by diverse agents including cell growth and differentiat ...201323764399
toxic effects of chlorpyrifos on lysozyme activities, the contents of complement c3 and igm, and igm and complement c3 expressions in common carp (cyprinus carpio l.).chlorpyrifos is one of the organophosphate pesticides widely used in agricultural practices throughout world. it has resulted in a series of toxicological and environmental problems, such as impacts on many non-target aquatic species, including fish. in the present study, toxic effects of chlorpyrifos on lysozyme activities, contents of igm and complement c3, and the expressions of igm and complement c3 at mrna level in common carp were evaluated by acute exposure of 15 (1/10 lc50) or 75μgl(-1) ...201323769463
β-glucan enriched bath directly stimulates the wound healing process in common carp (cyprinus carpio l.).wound healing is a complex and well-organized process in which physiological factors and immune mechanisms are involved. a number of different immune modulators have been found to enhance the non-specific defence system in vertebrates, among which β-glucans are the most powerful and extensively investigated. the aim of the present study was to investigate the biological impact of two different commercially available β glucan containing products on the wound healing process in carp. throughout a ...201323770625
dietary choline modulates immune responses, and gene expressions of tor and eif4e-binding protein2 in immune organs of juvenile jian carp (cyprinus carpio var. jian).the present work evaluates the effects of various levels of dietary choline on immune parameters, immune-related gene expression and protection against aeromonas hydrophila (ah) in juvenile jian carp (cyprinus carpio var. jian). fish were fed with six different experimental diets containing graded levels of choline at 165 (choline-deficient control), 310, 607, 896, 1167 and 1820 mg kg(-1) diet for 65 days. at the end of the feeding trail, fish were challenged with ah and mortalities were recorde ...201323774323
cdna cloning of glucose-6-phosphate isomerase from crucian carp (carassius carassius) and expression of the active region as myofibril-bound serine proteinase inhibitor in escherichia coli.glucose-6-phosphate isomerase (gpi) (ec 5.3.1.9) can act as a myofibril-bound serine proteinase (mbsp) inhibitor (mbspi) in fish. in order to better understand the biological information of the gpi and its functional domain for inhibiting mbsp, the cdna of gpi was cloned from crucian carp (carassius carassius) with rt-pcr, nested-pcr and 3'-race. the result of sequencing showed that the gpi cdna had an open reading frame of 1662bp encoding 553 amino acid residues. after constructing and comparin ...201423774640
short and long-term effects of clofibric acid and diclofenac on certain biochemical and ionoregulatory responses in an indian major carp, cirrhinus mrigala.extensive use of pharmaceuticals in human and veterinary medicine and aquaculture practices pose a serious threat to aquatic organisms. in the present investigation, cirrhinus mrigala an indian major carp was exposed to different concentrations of clofibric acid (ca) and diclofenac (dcf) and certain biochemical and ionoregulatory responses were assessed under short and long term exposures. during short-term (96h) exposure period, plasma glucose and sodium (na(+)) levels were increased at all con ...201323777676
evaluation of food chain transfer of the antibiotic oxytetracycline and human risk assessment.there has been recent concern regarding the possibility of antibiotics entering the aquatic food chain and impacting human consumers. this work reports experimental results of the bioconcentration of the antibiotic oxytetracycline (otc) by the asian watermeal plant (wolffia globosa hartog & plas) and bioaccumulation of otc in watermeal and water by the seven-striped carp (probarbus jullieni). they show, for the first time, the extent to which otc is able to transfer from water to plant to fish a ...201323790827
detoxification of jatropha curcas kernel cake by a novel streptomyces fimicarius strain.a huge amount of kernel cake, which contains a variety of toxins including phorbol esters (tumor promoters), is projected to be generated yearly in the near future by the jatropha biodiesel industry. we showed that the kernel cake strongly inhibited plant seed germination and root growth and was highly toxic to carp fingerlings, even though phorbol esters were undetectable by hplc. therefore it must be detoxified before disposal to the environment. a mathematic model was established to estimate ...201323792974
prostanoids-induced dispersion in the melanophores of a carp labeo rohita (ham.).effects of three prostaglandins (i.e., prostanoids) and one of its precursors, arachidonic acid, were examined on the melanophores of the fish labeo rohita (ham.). pge₁, pge₂, pgf(2α) and arachidonic acid elicit a concentration-related dispersion in the fish melanophores. in vitro analysis of melanophores was performed through incubation of the isolated fish scales in different agonists and antagonists solutions. dispersal effect of prostanoids may be mediated directly through the typical recept ...201423801365
anti-platelet-activating factor, antibacterial, and antiradical activities of lipids extract from silver carp brain.epidemiological studies have verified the protective role of fish lipids in cardiovascular diseases. however, the effects of fish lipids on health boost remain undefined. large amounts of by-products, such as fish brain which contains high level of lipids, are produced with silver carp processing. fish brain is rich in bioactive lipids which are overwhelmingly effective in preventing cardiovascular diseases. the aim of this study was to elucidate the pharmacological activities of silver carp bra ...201323805935
molecular characterization of five steroid receptors from pengze crucian carp and their expression profiles of juveniles in response to 17α-ethinylestradiol and 17α-methyltestosterone.pengze crucian carp (carassius auratus var. pengze, pcc), a triploid gynogenetic fish, was used in this study to investigate the cross-talk between edcs and steroid receptors. the full-length cdnas of five steroid receptors (esr1, er alpha2, esr2a, esr2b, ar) and partial cdna of vtg b were isolated. the tissue distributions of these genes were analyzed in adult fish by qrt-pcr. then the expression profiles of five steroid receptors (esrs and ar) and vtg b were detected in the juveniles exposed t ...201323806426
immunity to ichthyophthirius infections in fish: a synopsis.ichthyophthirius multifiliis is a ciliated protozoan parasite that infects freshwater fish. it has been the subject of both applied and basic research for over 100years, which can be attributed to its world-wide distribution and its significant economic impact on both food and aquarium fish production. i. multifiliis serves as a model for studies in fish on innate and acquired immunity, as well as on mucosal immunity. although an obligate parasite, i. multifiliis is relatively easily passaged fr ...201423810781
phenotypic changes in cyprinus carpiovar var. jian introduced by sperm-mediated transgenesis of rearranged homologous dna fragments.common carp, specifically the jian variety (cyprinus carpiovar var. jian), is an important chinese and global aquatic stock for commercial foodstuff. homologous recombination of carp gene sequences has been widely used in population genetics to broadly screen for beneficial phenotypical variations, thus optimizing artificially engineered carp stocks with jian variety and native stock varieties. random rearrangement of homologous dna fragments from parent specimens of c. carpiovar var. jian were ...201323824532
carp-1 functional mimetics: a novel class of small molecule inhibitors of medulloblastoma cell growth.medulloblastomas (mbs) constitute an aggressive class of intracranial pediatric tumors. current multimodality treatments for mbs include surgery, ionizing radiation, and chemotherapy. toxic side effects of therapies coupled with high incidence of recurrence and the metastatic spread warrant development of more effective, less toxic therapies for this disease. carp-1/ccar1 is a peri-nuclear phospho-protein that is a co-activator of the cell cycle regulatory anaphase promoting complex/cyclosome (a ...201323826121
impact of the introduction of drug eluting stents on clinical outcomes in patients undergoing percutaneous and surgical coronary artery revascularisation procedures in western australia.increasing rates of percutaneous coronary intervention (pci) and decreasing rates of coronary artery bypass graft (cabg) surgery followed the introduction of drug eluting stents in western australia in 2002. we assessed the impact of these changes on one-year outcomes for the total population of patients undergoing coronary artery revascularisation procedures (carp) in western australia between 2000-2004.201323826870
medaka haploid embryonic stem cells are susceptible to singapore grouper iridovirus as well as to other viruses of aquaculture fish species.viral infection is a challenge in high-density aquaculture, as it leads to various diseases and causes massive or even complete loss. the identification and disruption of host factors that viruses utilize for infection offer a novel approach to generate viral-resistant seed stocks for cost-efficient and sustainable aquaculture. genetic screening in haploid cell cultures represents an ideal tool for host factor identification. we have recently generated haploid embryonic stem (es) cells in the la ...201323828270
oxidative burst and nitric oxide responses in carp macrophages induced by zymosan, macrogard(®) and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors.β-glucans are glucose polymers that are found in the cell walls of plants, bacteria, certain fungi, mushrooms and the cell wall of baker's yeast. in mammals, myeloid cells express several receptors capable of recognizing β-glucans, with the c-type lectin receptor dectin-1 in conjunction with toll-like receptor 2 (tlr2), considered key receptors for recognition of β-glucan. in our studies to determine the possible involvement of these receptors on carp macrophages a range of sources of β-glucans ...201323831551
the tapeworm atractolytocestus tenuicollis (cestoda: caryophyllidea)--a sister species or ancestor of an invasive a. huronensis?atractolytocestus tenuicollis (li, 1964) xi, wang, wu, gao et nie, 2009 is a monozoic, non-segmented tapeworm of the order caryophyllidea, parasitizing exclusively common carp (cyprinus carpio l.). in the current work, the first molecular data, in particular complete ribosomal internal transcribed spacer 2 (its2) and partial mitochondrial cytochrome c oxidase subunit i (cox1) on a. tenuicollis from niushan lake, wuhan, china, are provided. in order to evaluate molecular interrelationships within ...201323832640
production of oocytes of nile tilapia (oreochromis niloticus) for in vitro fertilization via hormonal treatments.only a few studies have described hormonal treatments for induction of synchronicity and gamete collection in nile tilapia (oreochromis niloticus), both important for assortative matings in breeding programmes and essential for polyploidy technologies. in this study, we compared the effectiveness of carp pituitary extract (cpe), nile tilapia pituitary extract (tpe), human chorionic gonadotropin (hcg) and gonadotropin-releasing hormone (gnrh) protocols on the induction of spawning and egg product ...201323834584
the effect of prolonged exercise training on swimming performance and the underlying biochemical mechanisms in juvenile common carp (cyprinus carpio).to investigate the effect of prolonged exercise training on swimming performance and the underlying biochemical mechanisms in juvenile common carp (cyprinus carpio), we measured the critical swimming speed (ucrit), the excess post-exercise oxygen consumption (epoc), the activity of red and white muscle enzymes [pyruvate kinase (pk), lactate dehydrogenase (ldh) and citrate synthase (cs)], the tissue substrates (glycogen and glucose content of muscle and liver) and metabolite (the lactate content ...201323838144
heavy metals contamination in water and three species of most consumed fish sampled from caspian sea, 2011.the present study is an attempt to evaluate the heavy metal contamination in the marine environment of the caspian sea of iran. the concentrations of zinc, chromium, cadmium, and lead were measured in water and dorsal muscle of fish sampled from 10 selected stations along gorgan coast using atomic absorption spectrophotometry during summer of 2011. the average concentrations of lead in water and fish samples (115.29 ± 14.78 and 113.23 ± 27.01 μg/l) were significantly higher than that of cadmium ...201323842607
molecular characterization of hepcidin gene in common carp (cyprinus carpio l.) and its expression pattern responding to bacterial challenge.hepcidin is a cysteine-rich cationic antimicrobial peptide (amp), which plays an important role in host innate immune system and iron regulation. a great many of hepcidin genes have been identified from vertebrates, including various fish species. here we report the cloning and identification of a hepcidin cdna from the liver of common carp (cyprinus carpio l.). the full-length cdna of the common carp hepcidin was 647 bp, which contained an orf of 276 bp encoding a prepropeptide of 91 amino acid ...201323851290
the relationship of cytotoxic and genotoxic damage with blood aluminum levels and oxidative stress induced by this metal in common carp (cyprinus carpio) erythrocytes.aluminum is one of the most abundant elements in nature and is used in diverse industrial processes. as a result, it contaminates aquatic ecosystems, inducing damage on associated biota. in fish, it has been observed to induce hypoxia, hypercapnia, metabolic acidosis and respiratory arrest. although there is little information on al-induced cytotoxicity and dna damage, this type of studies are essential in order to identify the mechanisms of action of this metal. the cytotoxic and genotoxic effe ...201323856122
close association between paralogous multiple isomirs and paralogous/orthologues mirna sequences implicates dominant sequence selection across various animal species.micrornas (mirnas) are crucial negative regulators of gene expression at the post-transcriptional level. next-generation sequencing technologies have identified a series of mirna variants (named isomirs). in this study, paralogous isomir assemblies (from the mirna locus) were systematically analyzed based on data acquired from deep sequencing data sets. evolutionary analysis of paralogous (members in mirna gene family in a specific species) and orthologues (across different animal species) mirna ...201323856130
the il-10 homologue encoded by cyprinid herpesvirus 3 is essential neither for viral replication in vitro nor for virulence in vivo.cyprinid herpesvirus 3 (cyhv-3), a member of the family alloherpesviridae, is the causative agent of a lethal disease in common and koi carp. cyhv-3 orf134 encodes an interleukin-10 (il-10) homologue. the present study was devoted to this orf. transcriptomic analyses revealed that orf134 is expressed as a spliced gene belonging to the early-late class. proteomic analyses of cyhv-3 infected cell supernatant demonstrated that the orf134 expression product is one of the most abundant proteins of th ...201323865540
isolation and partial characterization of a novel virus from different carp species suffering gill necrosis - ultrastructure and morphogenesis.two isolates of a novel enveloped rna virus were obtained from carp and koi carp with gill necrosis. both isolates behaved identically and could be propagated in different cyprinid cell lines forming large syncytia. the virus was sensitive to lipid solvents and neither exhibited haemadsorption/haemagglutination nor reverse transcriptase activity. mature virus particles displayed a spherical shape with diameter of 100-350 nm after negative staining and 100-300 nm in ultrathin sections, covered by ...201423865968
brain h2a.z: the long and the short.the role of histone variants and their specific post-translational modifications (ptms) in the epigenetic regulation of gene expression is still poorly understood. a paper published by simonet and colleagues in epigenetics and chromatin describes a new h2a.z subtype that is specific for brain and pituitary in the carp and provides additional information about the functional epigenetic complexity of the ptms associated with histone h2a.z.201323866047
epigenetic regulation of the ribosomal cistron seasonally modulates enrichment of h2a.z and h2a.zub in response to different environmental inputs in carp (cyprinus carpio).the specific deposition of histone variants into chromatin is an important epigenetic mechanism that contributes to gene regulation through chromatin architectural changes. the histone variant h2a.z is essential in higher eukaryotes, and its incorporation within chromatin is a relevant process for gene expression and genome stability. however, the dual positive and negative roles of h2a.z in gene regulation still remain unclear. we previously reported that acclimatization in common carp fish (cy ...201323866978
functional analysis of domain of unknown function (duf) 1943, duf1944 and von willebrand factor type d domain (vwd) in vitellogenin2 in zebrafish.vitellogenin (vg), the precursor of egg-yolk proteins in all oviparous organisms, shares a similar domain structure combination. in most cases, vg contains the vitellogenin_n domain, the domain of unknown function (duf) 1943, and the von willebrand factor type d domain (vwd), which are present in different forms of vg from both vertebrates and invertebrates. occasionally, a duf1944 domain is also present in between duf1943 and vwd in some vg proteins of vertebrates. recent studies have shown tha ...201323867754
assessment of surface water in the vicinity of fertilizer factory using fish and plants.the genotoxic and toxic potential of polluted surface water exposed to a fertilizer factory effluent was evaluated using assays with fish (cyprinus carpio) and plant (lemna minor) model organisms. beside classical physicochemical parameters, the contents of fluorides, some heavy metals and polycyclic aromatic hydrocarbons were analyzed as well. surface water caused inhibition of plant growth and decrease of photosynthetic pigment content. regarding dna damage and oxidative stress parameters, bot ...201323871567
immunity against selected piscine flagellates.this discussion is on immune response to amyloodinium ocellatum, cryptobia salmositica, trypanoplasma borreli and trypanosoma carassii. piscidin and histone-like proteins enhance innate resistance to amyloodinium. fish that are naturally resistant to cryptobia and trypanoplasma can be bred. cryptobia resistance in charr is controlled by a dominant mendelian locus and protection is via the alternative pathway of complement activation. studies on cryptobia-tolerant charr may lead to production of ...201423872230
epidemiology of cyprinid herpesvirus-3 infection in latently infected carp from aquaculture.cyprinid herpesvirus-3 (cyhv-3, koi herpesvirus, khv) is the causative agent of an economically important disease in carp. the mode of transmission of this virus, especially how the infectious agent is introduced into ponds de novo, is not known in detail. the aim of this study was to investigate the shedding of cyhv-3 from fish with latent infections, under aquaculture conditions. ponds in saxony, germany, with latently infected carp were examined at different times during the production cycle ...201323872854
cyhv-3: the third cyprinid herpesvirus.common carp (including ornamental koi carp) cyprinus carpio l. are ecologically and economically important freshwater fish in europe and asia. c. carpio have recently been endangered by a third cyprinid herpesvirus, known as cyprinid herpesvirus-3 (cyhv-3), the etiological agent of koi herpesvirus disease (khvd), which causes significant morbidity and mortality in koi and common carp. clinical and pathological signs include epidermal abrasions, excess mucus production, necrosis of gill and inter ...201323872859
anticarbamylated protein (anti-carp) antibodies are present in sera of juvenile idiopathic arthritis (jia) patients. 201323873877
molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (megalobrama amblycephala).β-defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. in this study, the part-length cdna sequences of two β-defensin genes (maβd-1, maβd-2) in blunt snout bream (megalobrama amblycephala) were identified. homology analysis showed that the cdna sequences of maβd-1 and maβd-2 had high similarities to those in common carp and zebrafish. real-time quantitative pcr results exhibited that expre ...201323876385
evaluation of the effects induced by dietary diphenyl diselenide on common carp cyprinus carpio.several diets employed in aquaculture are enriched with selenium (se), as it is a fundamental element to aquatic vertebrates. diphenyl diselenide [(phse)₂], which is a synthetic organoselenium compound, has been considered a potential antioxidant agent in different experimental models. thus, the aim of this study was to evaluate the effects of dietary diphenyl diselenide at concentrations of 1.5, 3.0, and 5.0 mg/kg for 60 days and to determine its optimal supplemental level for carp, cyprinus ca ...201423877622
molecular characterization and expression analysis of toll-like receptor 21 cdna from paralichthys olivaceus.toll-like receptor (tlr) is believed to play crucial role in host defense of pathogenic microbes in innate immune system. in the present study, the full-length cdna of paralichthys olivaceus toll-like receptor 21 (po-tlr21) was cloned by homology cloning and rapid amplification of cdna ends (race) technique. the po-tlr21 cdna sequence was 3687 bp, containing an open reading frame of 2922 bp encoding 973 amino acids. tmhmm and smart program analysis indicated that protein contained one transmembr ...201323880453
detrimental effects of metronidazole on the liver of freshwater common carp (cyprinus carpio l.).the widely used antibiotic metronidazole (mtz) was investigated for its toxic effects on the liver of the common carp (cyprinus carpio l.). the fish were exposed to 0.1, 0.5, and 2.5 mg/l mtz in water for 30 days, and parameters that are indicative of liver damage and oxidative stress were measured. mtz increased liver ethoxyresorufin-o-deethylase, malondialdehyde, and glutathione levels, and elevated the activities of superoxide dismutase, catalase, aspartate aminotransferase, and alanine amino ...201323884169
changes in apoptotic rate and cell viability in three fish epidermis cultures after exposure to nonylphenol and to a wastewater sample containing low concentrations of nonylphenol.three types of epidermal cultures of fish were used for toxicological investigations, a primary cell culture and a tissue culture prepared from the rainbow trout oncorhynchus mykiss walbaum and the cell line epc, derived from a skin tumour of the carp cyprinus carpio l. two studies were carried out to compare the different culture systems. in the first cultures were incubated with nonylphenol and in the second set of experiments the cell cultures were exposed to a wastewater sample containing lo ...200023889222
effects of a dietary β-(1,3)(1,6)-d-glucan supplementation on intestinal microbial communities and intestinal ultrastructure of mirror carp (cyprinus carpio l.).to assess the effects of dietary saccharomyces cerevisiae β-(1,3)(1,6)-d-glucan supplementation (macrogard(®)) on mirror carp (cyprinus carpio l.) intestinal microbiota and ultrastructure of the enterocyte apical brush border.201323889777
comparative study on effects of dietary with diphenyl diselenide on oxidative stress in carp (cyprinus carpio) and silver catfish (rhamdia sp.) exposed to herbicide clomazone.the study investigated the capacity of diphenyl diselenide [(phse)2] (3.0mg/kg), on reduce the oxidative damage in liver, gills and muscle of carp and silver catfish exposed to clomazone (192h). silver catfish exposed to clomazone showed increased thiobarbituric acid-reactive substance (tbars) in liver and muscle and protein carbonyl in liver and gills. furthermore, clomazone in silver catfish decrease non-protein thiols (npsh) in liver and gills and glutathione peroxidase and ascorbic acid in l ...201323892285
the primary culture of mirror carp snout and caudal fin tissues and the isolation of koi herpesvirus.the explosive koi herpesvirus (khv) epidemic has caused the deaths of a large number of carp and carp variants and has produced serious economic losses. the mirror carp (cyprinus carpio var. specularis) exhibits strong environmental adaptability and its primary cells can be used to isolate khv. this study utilized the tissue explant method to systematically investigate primary cell culture conditions for mirror carp snout and caudal fin tissues. we demonstrated that cells from these two tissue t ...201323893087
Refresh
firstprev
...61626364656667686970...
nextlast
Displaying items 6001 - 6100 of 7554