Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of olive powder and high hydrostatic pressure on the inactivation of bacillus cereus spores in a reference medium. | the aim of this work was to study the effect of olive powder combined with high hydrostatic pressure (hhp) on the inactivation of bacillus cereus spores, to use it as an additional control hurdle in beverages pasteurised by this technology. with this purpose, reference medium prepared at different concentrations of olive powder was inoculated with b. cereus spores and subjected to different pressure treatments. the outgrowth capacity of the treated spores was then determined at 20-¦c and 32-¦c. ... | 2011 | 21381901 |
| emetic disease caused by bacillus cereus after consumption of tuna fish in a beach club. | several vomiting episodes were reported in december 2007 by the management of a beach club in calvi+á. the subsequent case investigation confirmed tuna fish consumption a few hours before onset of emesis in all cases. microbiological analyses detected high bacterial levels in ready-to-eat fish samples, indicating inappropriate cooking procedures. more important, elevated levels of bacillus cereus were present both in raw and cooked fish. no other pathogens were detected, indicating b. cereus as ... | 2011 | 21381943 |
| maklamicin, an antibacterial polyketide from an endophytic micromonospora sp. | a new spirotetronate-class polyketide, maklamicin (1), was isolated from the culture extract of an endophytic actinomycete of the genus micromonospora. the structure and relative configuration of 1 were elucidated by interpretation of nmr and other spectroscopic data, and the absolute configuration was determined using the modified mosher method. maklamicin (1) showed strong to modest antimicrobial activity against gram-positive bacteria. | 2011 | 21388191 |
| partial characterization of bacteriocins produced by two lactobacilus strains with probiotic properties. | the probiotic characteristics of lactobacillus brevis bg18 and lb. plantarum bg33, isolated from traditional turkish tulum cheese were assessed. these two bacteriocinproducer strains exhibited good probiotic characteristics such as resistance in media containing 0.3% bile salt, pepsin (3 mg mlôü+-¦), and pancreatine (1 mg mlôü+-¦) as well as acid resistance at ph 2. they were also adhered to caco-2 epithelial cells in a manner comparable to escherichia coli lmg3083 (etec) and salmonella typhimur ... | 2011 | 21388923 |
| improving the ethanol yield by reducing glycerol formation using cofactor regulation in saccharomyces cerevisiae. | to increase ethanol yield and decrease glycerol production in saccharomyces cerevisiae, the strategies of direct cofactor-regulation were explored. during anaerobic batch fermentations, the yeast expressing bacillus cereus gapn gene, encoding non-phosphorylating nadp(+)-dependent glyceraldehyde-3-phosphate dehydrognease, produced 73.8 g ethanol l(-1), corresponding to 96% of theoretical maximum yield compared to 92% for the wild type. the yeast expressing escherichia coli frda gene encoding the ... | 2011 | 21400237 |
| the role of two families of bacterial enzymes in putrescine synthesis from agmatine via agmatine deiminase. | putrescine, one of the main biogenic amines associated to microbial food spoilage, can be formed by bacteria from arginine via ornithine decarboxylase (odc), or from agmatine via agmatine deiminase (agdi). this study aims to correlate putrescine production from agmatine to the pathway involving n-carbamoylputrescine formation via addi (the agua product) and n-carbamoylputrescine amidohydrolase (the agub product), or putrescine carbamoyltransferase (the ptca product) in bacteria. pcr methods were ... | 2010 | 21404211 |
| [biodiversity of mesophilic microbial community bynd-8 capability of lignocellulose degradation and its effect on biogas production]. | the biodiversity of a mesophilic microbial community bynd-8 capable of degrading lignocellulose at 30 degrees c was detected using denaturing gradient gel electrophoresis (dgge) and the isolation of pure cultures, and the effect of the liquid of rice straw degradation by bynd-8 on biogas production was measured. six bacterial strains were isolated using peptone cellulose solution medium, and the highest similarities of their 16s rdna gene sequences to serratia sp. psgb 13, s. marcescens strain u ... | 2011 | 21404695 |
| regulation of toxin production by bacillus cereus and its food safety implications. | toxin expression is of utmost importance for the food-borne pathogen b. cereus, both in food poisoning and non-gastrointestinal host infections as well as in interbacterial competition. therefore it is no surprise that the toxin gene expression is tightly regulated by various internal and environmental signals. an overview of the current knowledge regarding emetic and diarrheal toxin transcription and expression is presented in this review. the food safety aspects and management tools such as te ... | 2011 | 21417966 |
| application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents. | a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ... | 2011 | 21419572 |
| in vitro antioxidant, anticholinesterase and antimicrobial activity studies on three agaricus species with fatty acid compositions and iron contents: a comparative study on the three most edible mushrooms. | the fatty acids of agaricus essettei, agaricus bitorquis and agaricus bisporus were investigated by using gc and gc-ms. the dominant fatty acids were found to be linoleic (61.82-67.29%) and palmitic (12.67-14.71%) acids among the 13 fatty acids detected in the oils. total unsaturation for the oils was calculated as 77.50%, 77.44%, and 79.72%, respectively. in vitro antioxidant, anticholinesterase and antimicrobial activities were also studied. the ethyl acetate extract of agaricus bitorquis show ... | 2011 | 21419821 |
| secondary cell wall polysaccharides from bacillus cereus strains g9241, 03bb87 and 03bb102 causing fatal pneumonia share similar glycosyl structures with the polysaccharides from bacillus anthracis. | secondary cell wall polysaccharides (scwps) are important structural components of the bacillus cell wall and contribute to the array of antigens presented by these organisms in both spore and vegetative forms. we previously found that antisera raised to bacillus anthracis spore preparations cross-reacted with scwps isolated from several strains of pathogenic b. cereus, but did not react with other phylogenetically related but nonpathogenic bacilli, suggesting that the scwp from b. anthracis and ... | 2011 | 21421577 |
| an eight-year report on the implementation of haccp in a university canteen: impact on the microbiological quality of meals. | an investigation aimed at assessing the microbiological quality of meals consumed at a university canteen after implementation of the haccp system and personnel training was carried out. cooked and warm-served products (74 samples), cooked and cold-served products (92 samples) and cold gastronomy products (63 samples) sampled from 2000 to 2007 underwent microbiological analyses. all the samples were tested for: samonella spp., listeria monocytogenes, total mesophilic aerobes, coliforms, escheric ... | 2011 | 21424969 |
| [microbiological evaluation of ready-to-eat foods manufactured by small costa rican industries]. | ready-to-eat (rte) foods are processed foodstuffs which have gained popularity in recent times because they can be ingested without further thermic treatments. in this work, the microbiological quality and safety of 90 samples of rte foods manufactured by small costa rican industries was determined to evaluate whether they represent a public health risk. twenty-six samples of pickled vegetables, 18 dips, 18 salads, and 12 sweet treats were studied. each sample was analyzed with regard to its ph, ... | 2010 | 21427886 |
| identification and safety evaluation of bacillus species occurring in high numbers during spontaneous fermentations to produce gergoush, a traditional sudanese bread snack. | gergoush is a naturally fermented sudanese bread snack produced in three fermentation steps (primary starter, adapted starter and final dough), followed by three baking steps for a half to one hour at above 200 °c. this study examines the microbiota of two sets of fermentations performed at a traditional production site in khartoum, sudan in 2006 and 2009, respectively. in 2006 four different milk/legume based primary starters (faba bean, chick pea, lentil and white bean) were sampled in order t ... | 2011 | 21429611 |
| notes from the field: contamination of alcohol prep pads with bacillus cereus group and bacillus species--colorado, 2010. | in october 2010, a child at the children's hospital (tch) in aurora, colorado, with newly diagnosed leukemia developed clinical sepsis 24 hours after insertion of an implanted vascular access device. the child also developed extensive cellulitis at the insertion site, requiring surgical debridement, intensive care, antibiotics, prolonged wound management, and outpatient treatment. cultures of the child's blood and tissue specimens grew bacillus cereus. an investigation found neither breach of in ... | 2011 | 21430639 |
| development and validation of stable reference materials for food microbiology using bacillus cereus and clostridium perfringens spores. | to develop a new type of microbiological reference materials (rms), displaying long-term stability at room temperature. the purpose was to produce and validate two batches of rms for the enumeration of bacillus cereus and clostridium perfringens. | 2011 | 21435123 |
| genetic diversity, antimicrobial resistance and toxigenic profiles of bacillus cereus isolated from food in brazil over three decades. | bacillus cereus is an ever-present problem. it is widely distributed in several environments such as soil and plants and is commonly isolated from food and additives. in this study we analyzed 97 foodborne b. cereus sensu stricto strains isolated in brazil in the 1980's, 1990's and 2000's in order to investigate the genetic diversity (assessed by rep-pcr), antimicrobial resistance and toxigenic profiles (presence of hbla, hblc and hbld; nhea, nheb and nhec as well as cytk, ces and entfm genes) o ... | 2011 | 21440319 |
| kinetics of germination of wet-heat-treated individual spores of bacillus species, monitored by raman spectroscopy and differential interference contrast microscopy. | raman spectroscopy and differential interference contrast (dic) microscopy were used to monitor the kinetics of nutrient and nonnutrient germination of multiple individual untreated and wet-heat-treated spores of bacillus cereus and bacillus megaterium, as well as of several isogenic bacillus subtilis strains. major conclusions from this work were as follows. (i) more than 90% of these spores were nonculturable but retained their 1:1 chelate of ca²+ and dipicolinic acid (cadpa) when incubated in ... | 2011 | 21441336 |
| a membrane-based, high-efficiency, microfluidic debubbler. | in many lab-on-chip applications, it is necessary to remove bubbles from the flow stream. existing bubble removal strategies have various drawbacks such as low degassing efficiency, long degassing time, large dead volumes, sensitivity to surfactants, and the need for an external vacuum or pressure source. we report on a novel, simple, robust, passive, nozzle-type, membrane-based debubbler that can be readily incorporated into microfluidic devices for rapid degassing. the debubbler is particularl ... | 2011 | 21445396 |
| investigating the genome diversity of b. cereus and evolutionary aspects of b. anthracis emergence. | here we report the use of a multi-genome dna microarray to investigate the genome diversity of bacillus cereus group members and elucidate the events associated with the emergence of bacillus anthracis the causative agent of anthrax-a lethal zoonotic disease. we initially performed directed genome sequencing of seven diverse b. cereus strains to identify novel sequences encoded in those genomes. the novel genes identified, combined with those publicly available, allowed the design of a "species" ... | 2011 | 21447378 |
| antagonistic bacteria from live corals, tuticorin coastal waters, southeastern india. | the objective of this study is to isolate and production of secondary metabolites with bioactive substances by coral reef (acropora formosa and favia palida) associated bacteria was carried out from the tuticorin coastal waters, southeastern india. the isolated coral associated bacteria were found to have an antagonistic effect against 10 human pathogens. the pathogens were escherichia coli, shigella dysentriae, staphylococcus epidermidis, s. aureus, klebsiella pneumoniae, pseudomonas aerogenosa ... | 2011 | 21454167 |
| invertebrate pathogenicity and toxin-producing potential of strains of bacillus thuringiensis endemic to antarctica. | several strains of bacillus thuringiensis were previously isolated from soil in antarctica and appeared to have physiological adaptations to this cold, nutrient-poor environment. in spite of this they could produce abnormally large, parasporal crystals under laboratory conditions. here, they have been further characterised for toxin genes and invertebrate pathogenicity. all of the strains were positive in pcr assays for the cry1aa and cry2 genes. this was confirmed by sequence analysis and the p ... | 2011 | 21457716 |
| biofilm inhibition and antimicrobial action of lipopeptide biosurfactant produced by heavy metal tolerant strain bacillus cereus nk1. | biosurfactants are worthful microbial amphiphilic molecules with efficient surface-active and biological properties applicable to several industries and processes. among them lipopeptides represent a class of microbial surfactants with increasing scientific, therapeutic and biotechnological interests. a heavy metal tolerant bacillus strain has been isolated and the biofilm inhibition and antimicrobial activity of biosurfactant produced by the strain have been studied. biosurfactant production wa ... | 2011 | 21458961 |
| antimicrobial activity of plant essential oils against bacterial and fungal species involved in food poisoning and/or food decay. | the currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. the purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the national institute of research-development for chemistry and petrochemistry, bucharest to be used for the fu ... | 2010 | 21462837 |
| a rapd-pcr method for the rapid detection of bacillus cereus. | distinction of bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. from our previous work on rapd profiles of bacilli, we found a possibility that b. cereus strains could be distinguished from other bacilli. in this work, rapd-pcr profiles of b. cereus strains were obtained using a 10-mer (s30) as a primer, and a b. cereus specific 0.91-kb band was produced from all tested strains. the rapd-pcr procedure also successfully detected ... | 2011 | 21464598 |
| rapid identification of emetic bacillus cereus by immunochromatography. | the immunochromatographic assay, which targets a marker protein co-expressed during the synthesis of cereulide by an emetic bacillus cereus strain, was used for easily, rapidly and specifically identifying the emetic strains among b. cereus strains from various materials associated with food poisonings. all 50 of the emetic strains showed a positive reaction to the assay, but all 50 diarrheal strains had a negative reaction. the bacterial counts of 108 cfu/ml in enrichment broth and 109 cfu/ml i ... | 2011 | 21467628 |
| crystal structure of a phosphorylation-coupled saccharide transporter. | saccharides have a central role in the nutrition of all living organisms. whereas several saccharide uptake systems are shared between the different phylogenetic kingdoms, the phosphoenolpyruvate-dependent phosphotransferase system exists almost exclusively in bacteria. this multi-component system includes an integral membrane protein eiic that transports saccharides and assists in their phosphorylation. here we present the crystal structure of an eiic from bacillus cereus that transports diacet ... | 2011 | 21471968 |
| resistance and biosorption mechanism of silver ions by bacillus cereus biomass. | biosorption of silver ions onto bacillus cereus biomass was investigated. overall kinetic experiments were performed for the determination of the necessary contact time for the attainment of equilibrium. it was found that the overall biosorption process was best described by pseudo second-order kinetic model. the crystals detected by scanning electron microscope and x-ray photoelectron spectroscopy suggested the precipitation was a possible mechanism of biosorption. the molecular genetics of sil ... | 2011 | 21476348 |
| phenolic composition and antimicrobial and antiquorum sensing activity of an ethanolic extract of peels from the apple cultivar annurca. | abstract this study investigated the phenolic composition and antioxidant capacity of an ethanolic extract from the peel of the annurca (malus domestica var. annurca), which is widespread in the campania region of southern italy. the antimicrobial effect of the extract on different pathogens was also examined. the potential antiquorum sensing activity of the extract was tested by using the microorganism chromobacterium violaceum. ultra-performance liquid chromatography revealed that rutin, epica ... | 2011 | 21476926 |
| growth characteristics and development of a predictive model for bacillus cereus in fresh wet noodles with added ethanol and thiamine. | response surface methodology was used to determine growth characteristics and to develop a predictive model to describe specific growth rates of bacillus cereus in wet noodles containing a combination of ethanol (0 to 2% [vol/wt]) and vitamin b(1) (0 to 2 g/liter). b. cereus f4810/72, which produces an emetic toxin, was used in this study. the noodles containing b. cereus were incubated at 10°c. the growth curves were fitted to the modified gompertz equation using nonlinear regression, and the g ... | 2011 | 21477484 |
| [epidemiological investigation for outbreak of food poisoning caused by bacillus cereus among the workers at a local company in 2010]. | in july 2 2010, a diarrhea outbreak occurred among the workers in a company in gyeungju city, korea. an epidemiological investigation was performed to clarify the cause and transmission route of the outbreak. | 2011 | 21483225 |
| purification and characterization of a new bacillus thuringiensis bacteriocin active against listeria monocytogenes, bacillus cereus and agrobacterium tumefaciens. | this study reports on the identification, characterization and purification of a new bacteriocin, named bacthuricin f103, from a bacillus thuringiensis strain bupm103. bacthuricin f103 production began in the early exponential phase and reached a maximum in the middle of the same phase. two chromatographic methods based on high performance liquid chromatography and fast protein liquid chromatography systems were used to purify bacthuricin f103. sodium dodecyl sulphate-polyacrylamide gel electrop ... | 2011 | 21487734 |
| antimicrobial activity of blumea balsamifera (lin.) dc. extracts and essential oil. | leaves of blumea balsamifera (lin.) dc. are used in traditional thai and chinese medicine for the treatment of septic wounds and other infections. in this study, the essential oil, hexane, dichloromethane and methanol extracts of these leaves were evaluated for antibacterial and antifungal activities using the disc diffusion assay and agar microdilution method. the essential oil was the most potent, with a minimum inhibitory concentration (mic) of 150 µg ml(-1) against bacillus cereus and an mic ... | 2011 | 21500092 |
| the proof of the pudding is in the eating: an outbreak of emetic syndrome after a kindergarten excursion, berlin, germany, december 2007. | an outbreak of food poisoning (emetic syndrome) occurred in three kindergartens (a, b and c) in berlin, germany, on 3 december 2007 after an excursion during which food was served. we conducted a retrospective cohort study among the kindergarten children and personnel who participated in the trip. the overall attack rate among the 155 participants was 30%. it was 31% among the 137 children (aged two to six years) and 17% among adults (n=18). the consumption of rice pudding was significantly asso ... | 2011 | 21507320 |
| risk evaluation and management to reaching a suggested fso in a steam meal. | steam meals are ready-to-eat meals composed of raw and semi-cooked ingredients, which get cooked while microwave heating. in this study, an indian style meal was selected, chicken tandoori, from two different producers. these meals were first evaluated with the risk ranger® to identify the main foodborne pathogens risks, which were listeria monocytogenes, salmonella typhimurium and bacillus cereus. thereafter, quantitative microbiology was applied using different models and verified with growth ... | 2011 | 21511122 |
| the effects of glutaraldehyde on the control of single and dual biofilms of bacillus cereus and pseudomonas fluorescens. | glutaraldehyde (glut) was evaluated for control of single and dual species biofilms of bacillus cereus and pseudomonas fluorescens on stainless steel surfaces using a chemostat system. the biofilms were characterized in terms of mass, cell density, total and matrix proteins and polysaccharides. the control action of glut was assessed in terms of inactivation and removal of biofilm. post-biocide action was characterized 3, 7, 12, 24, 48 and 72 h after treatment. tests with planktonic cells were a ... | 2011 | 21512918 |
| crystal structure of pduo-type atp:cob(i)alamin adenosyltransferase from bacillus cereus in a complex with atp. | atp:cobalamin adenosyltransferases catalyze the transfer a 5'-deoxyadenosyl moiety from atp to cob(i)alamin in the synthesis of the co-c bond of coenzyme b(12). there are three types of adenosyltransferases, coba, pduo and eutt. among these adenosyltransferases, the pduo-type adenosyltransferases is the most widely distributed enzyme. structural comparisons between apo bcpduo and bcpduo in complex with mgatp revealed that the n-terminal strands of both structures were ordered, which is in contra ... | 2011 | 21514283 |
| antibacterial activity of different honeys against pathogenic bacteria. | to study the antimicrobial activity of honey, 60 samples of various botanical origin were evaluated for their antimicrobial activities against 16 clinical pathogens and their respective reference strains. the microbiological quality of honeys and the antibiotic susceptibility of the various isolates were also examined. the bioassay applied for determining the antimicrobial effect employs the well-agar diffusion method and the estimation of minimum active dilution which produces a 1mm diameter in ... | 2011 | 21524711 |
| effect of benzoic acid and combination of benzoic acid with a probiotic containing bacillus cereus var. toyoi in weaned pig nutrition. | the purpose of this study was to assess the efficacy of a probiotic containing bacillus cereus var. toyoi spores (toyocerin) and benzoic acid (vevovitall) on growth performance and diarrhoea in weaning pigs, against negative controls. the trial groups were as follows: (a) nc group (negative controls): no treatment (b) toyo group: same feed as in the controls plus toyocerin at a dose of 1 x 10(9) bacillus cereus var. toyoi spores/kg feed, (c) ba group: same feed as in the controls plus vevovitall ... | 2011 | 21528721 |
| the nudix hydrolase cdp-chase, a cdp-choline pyrophosphatase, is an asymmetric dimer with two distinct enzymatic activities. | a nudix enzyme from bacillus cereus (accession no. np_831800) catalyzes the hydrolysis of cdp-choline to produce cmp and phosphocholine. here we show that in addition, the enzyme has a 3'→5' rna exonuclease activity. the structure of the free enzyme, determined to 1.8 å resolution, shows that the enzyme is an asymmetric dimer. each monomer consists of two domains, an n-terminal helical domain and a c-terminal nudix domain. the n-terminal domain is placed relative to the c-terminal domain in such ... | 2011 | 21531795 |
| synthesis, antibacterial activity and quantum-chemical studies of novel 2-arylidenehydrazinyl-4-arylthiazole analogues. | a new series of 2-arylidenehydrazinyl-4-arylthiazole derivatives (2a-k) was designed and synthesized through a rapid, simple, and efficient methodology in excellent isolated yield. these compounds were screened for in vitro antimicrobial activities against eight bacteria, e.g. bacillus cereus, staphylococcus aureus, bacillus subtilis, bacillus megaterium, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhi, escherichia coli, and three fungi e.g. aspergillus oryzae, candida albicans, a ... | 2011 | 21532194 |
| synthesis, characterization and in vitro antimicrobial evaluation of new compounds incorporating oxindole nucleus. | new compounds incorporating with the oxindole nucleus were synthesized via the reaction of substituted isatins [5-methyl-, 5-chloro- and 1-hydroxymethyl isatins] with different nucleophiles. the structures of the newly compounds were elucidated on the basis of ftir, (1)h nmr, (13)cmr spectral data, gc/ms and chemical analysis. investigation of antimicrobial activity of the new compounds was evaluated using broth dilution technique in terms of minimal inhibitory concentration (mic) count against ... | 2011 | 21534861 |
| evaluation of antibacterial activity of 3-butenyl, 4-pentenyl, 2-phenylethyl, and benzyl isothiocyanate in brassica vegetables. | this study investigated antibacterial activities of 4 isothiocyanates (3-butenyl, 4-phentenyl, 2-phenylethyl, and benzyl isothiocyanate) against 4 gram-positive bacteria (bacillus cereus, bacillus subtilis, listeria monocytogenes, and staphylococcus aureus) and 7 gram-negative bacteria (aeromonas hydrophila, pseudomonas aeruginosa, salmonella choleaesuis, salmonella enterica, serratia marcescens, shigella sonnei, and vibrio parahaemolyticus) by an agar disc diffusion assay. benzyl isothiocyanate ... | 2010 | 21535549 |
| toxin genes profiles and toxin production ability of bacillus cereus isolated from clinical and food samples. | bacillus cereus can cause diarrheal and emetic type of food poisoning but little study has been done on the main toxins of food poisoning caused by b. cereus in korea. the objective of this study is to characterize the toxin gene profiles and toxin-producing ability of 120 b. cereus isolates from clinical and food samples in korea. the detection rate of nheabc, hblcda, entfm, and cytk enterotoxin gene among all b. cereus strains was 94.2, 90.0, 65.8, and 52.5%, respectively. the ces gene encodin ... | 2011 | 21535727 |
| gastrointestinal passage of bacillus cereus. | 2011 | 21539184 | |
| synthesis and antimicrobial activities of hexahydroimidazo[1,5-a]pyridinium bromides with varying benzyl substituents. | variously substituted benzyl bromides were employed to quaternize hexahydrobenzylimidazo[1,5-a]pyridine (a) and the resulting bromides (1-11) were evaluated for their in vitro antimicrobial activity against 10 pathogenic microorganisms: staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, micrococcus luteus, proteus vulgaris, escherichia coli, salmonella typhimurium, klebsiella pneumonia, candida albicans and candida krusei. antimicrobial activities were surprisingly high (mic: 0. ... | 2011 | 21543140 |
| growth inhibition and induction of stress protein, groel, of bacillus cereus exposed to antibacterial peptide isolated from bacillus subtilis sc-8. | this study was conducted to investigate the antibacterial effect of bsap-254 on bacillus cereus with the induced stress proteins. the bsap-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, bacillus subtilis sc-8. it had a narrow spectrum of activity against b. cereus group. the growth inhibitory effect of bsap-254 (50 μg/ml) reduced the population of b. cereus from >10(8) to 10(4) colony-forming units per milliliter within 30 min. in b. cereus exposed to bsap-254, 14 int ... | 2011 | 21544555 |
| occurrence of microorganisms of public health and spoilage significance in fruit juices sold in retail markets in greece. | fruit juices are an important part of the modern diet in many countries. however, few data are available concerning the microbiological quality of the fruit juices sold in greece. using standard microbiological procedures, we conducted a bacteriological survey of commercially sold, pasteurized, shelf-stable fruit juices from retail markets. a total of 120 samples of fruit juices sold in various retail markets were examined for their bacteriological quality. the ph of the tested juices was 2.4-4. ... | 2011 | 21545842 |
| production and properties of two collagenases from bacteria and their application for collagen extraction. | two collagenolytic protease (collagenase) producing bacteria, a gram positive bacillus cereus cna1 and a gram negative klebsiella pneumoniae cnl3, were isolated under alkaline and acidic conditions, respectively. the production of collagenase by these two bacteria was optimized. glycerol was the suitable carbon source for collagenase production by both strains. the optimal initial ph values for collagenase production by cna1 and cnl3 were 7.5 and 6.0, respectively, and the optimal temperature wa ... | 2011 | 21549225 |
| potential application of the nisin z preparation of lactococcus lactis w8 in preservation of milk. | aims: the aim of the study is to evaluate the effectiveness of the preparation of nisin z from lactococcus lactis w8-fermented milk in controlling the growth of spoilage bacteria in pasteurized milk. methods and results: spoilage bacteria isolated from pasteurized milk at 8 and 15°c were identified as enterococcus italicus, enterococcus mundtii, enterococcus faecalis, bacillus thuringiensis, bacillus cereus, lactobacillus paracasei, acinetobacter sp., pseudomonas fluorescens and enterobacter a ... | 2011 | 21554339 |
| bacillus cereus responses to acid stress. | coping with acid environments is one of the prerequisites for the soil saprophytic and human pathogenic lifestyle of bacillus cereus. this minireview highlights novel insights in the responses displayed by vegetative cells and germinating spores of b. cereus upon exposure to low ph as well as organic acids, including acetic acid, lactic acid and sorbic acid. insights regarding the possible acid-inflicted damage, physiological responses and protective mechanisms have been compiled based on single ... | 2011 | 21554514 |
| antibacterial peptides derived from caprine whey proteins, by digestion with human gastrointestinal juice. | peptides in caprine whey were identified after in vitro digestion with human gastrointestinal enzymes in order to determine their antibacterial effect. the digestion was performed in two continuing steps using human gastric juice (ph 2·5) and human duodenal juice (ph 8) at 37°c. after digestion the hydrolysate was fractionated and 106 peptides were identified. from these results, twenty-two peptides, located in the protein molecules, were synthesised and antibacterial activity examined. strong a ... | 2011 | 21554806 |
| antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease. | background: bioactive peptides might be released from precursor proteins through enzymatic hydrolysis. these molecules could be potentially employed in health and food products. in this investigation, ovine milk caseinate hydrolysates obtained with a novel microbial protease derived from bacillus sp. p7 were evaluated for antioxidant, antimicrobial, and angiotensin i-converting enzyme (ace)-inhibitory activities. results: antioxidant activity measured by the 2,2'-azino-bis-(3-ethylbenzothiazolin ... | 2011 | 21560133 |
| characterization of bacilli isolated from the confined environments of the antarctic concordia station and the international space station. | abstract bacillus and related genera comprise opportunist and pathogen species that can threaten the health of a crew in confined stations required for long-term missions. in this study, 43 bacilli from confined environments, that is, the antarctic concordia station and the international space station, were characterized in terms of virulence and plasmid exchange potentials. no specific virulence feature, such as the production of toxins or unusual antibiotic resistance, was detected. most of th ... | 2011 | 21563959 |
| follow-up of the bacillus cereus emetic toxin production in penne pasta under household conditions using liquid chromatography coupled with mass spectrometry. | two outbreak-related bacillus cereus emetic strains were investigated for their growth and cereulide production potential in penne pasta at 4, 8 and 25 °c during 7-day storage. cereulide production was detected and quantified by lc-ms method (lod of 1 ng/ml, loq of 5 ng/ml) and growth was determined by culture-based enumeration. inoculated b. cereus strains (10(5) cfu/g) were able to reach counts of more than 10(8) cfu/g and cereulide production of about 500 ng/g already after 3 days of storage ... | 2011 | 21569959 |
| isolation of glucosidase and phospholipase positive bacillus circulans on aloa medium. | aim: to report the growth of glucosidase and phospholipase positive bacteria on agar listeria according to ottaviani and agosti (aloa) different from listeria monocytogenes, listeria ivanovii and bacillus cereus. methods and results: raw water-buffalo milk was analysed according to en iso 11290. streaking of fraser broth on aloa resulted in green colonies with an opaque halo after 48 h at 30°c. colonies were transferred onto tryptone soya yeast extract agar and showed cultural characteristics ... | 2011 | 21575023 |
| bacillus cereus g9241 makes anthrax toxin and capsule like highly virulent b. anthracis ames but behaves like attenuated toxigenic nonencapsulated b. anthracis sterne in rabbits and mice. | bacillus cereus g9241 was isolated from a welder with a pulmonary anthrax-like illness. the organism contains two megaplasmids, pbcxo1 and pbc218. these plasmids are analogous to the b. anthracis ames plasmids pxo1 and pxo2 that encode anthrax toxins and capsule, respectively. here we evaluated the virulence of b. cereus g9241 as well as the contributions of pbcxo1 and pbc218 to virulence. b. cereus g9241 was avirulent in new zealand rabbits after subcutaneous inoculation and attenuated 100-fold ... | 2011 | 21576337 |
| successful surgical drainage and aggressive medical therapy in a preterm neonate with bacillus cereus meningitis. | bacillus cereus meningitis is a rare disease with a very high mortality rate in neonates. the authors present the rare case of a premature infant with b. cereus bacteremia and subsequent intracranial abscesses. in addition to aggressive medical therapy, surgical drainage was performed via a left frontal mini-craniotomy. at 15 months of age, the patient had mild developmental delay, cortical blindness, and sensorineural hearing loss. the clinical case is described and difficulties in the manageme ... | 2011 | 21577046 |
| physiological parameters of bacillus cereus marking the end of acid-induced lag phases. | during lag phases microbial cells adapt to their environment and prepare to proliferate. physiological parameters of b. cereus cells upon exposure to near-growth-boundary acid stress were investigated and markers for the transition between lag phase and growth were identified using fluorescent probes combined with flow cytometry. determination of cell counts and optical density revealed lag phases of 1h, 2h and 5h, in cultures shifted to ph 7, ph 5.3 (set with lactic acid) and ph 4.9 (set with s ... | 2011 | 21592605 |
| synthesis of gold and silver nanoparticles using purified urak. | this study aims at developing a new eco-friendly process for the synthesis of silver nanoparticles (agnps) and gold nanoparticles (aunps) using purified urak. urak is a fibrinolytic enzyme produced by bacillus cereus nk1. the enzyme was purified and used for the synthesis of aunps and agnps. the enzyme produced agnps when incubated with 1mm agno3 for 24h and aunps when incubated with 1mm haucl4 for 60h. but when naoh was added, the synthesis was rapid and occurred within 5min for agnps and 12h f ... | 2011 | 21592748 |
| sulfamethoxazole biodegradation and biotransformation in the water-sediment system of a natural river. | in this study, the biodegradation of sulfamethoxazole (smx) as affected by temperature, humic acid (ha) and smx concentrations was investigated by hplc-ms/ms analysis based on water-sediment batch experiments. the first order decay model (c=c(0)×exp (-kt)) was best fitted for smx biodegradation. smx degradation significantly increased with elevated temperature (degradation rate was 82.9% at 25°c vs. 40.5% at 4°c in sediment), ha contents (30mg/l of ha facilitated smx degradation rate at 90.1% vs ... | 2011 | 21596556 |
| monitoring the wet-heat inactivation dynamics of single spores of bacillus species using raman tweezers, differential interference contrast and nucleic acid dye fluorescence microscopy. | dynamic processes during wet heat treatment at 80-90°c of individual spores of bacillus cereus, bacillus megaterium and bacillus subtilis were investigated using dual-trap raman spectroscopy, differential interference contrast (dic) microscopy and nucleic acid stain (syto 16) fluorescence microscopy. during spore wet heat treatment, while spores' 1:1 chelate of ca(2+) with dipicolinic acid (cadpa) was released rapidly at a highly variable time t(lag), the levels of spore nucleic acids remained n ... | 2011 | 21602365 |
| enzymatic synthesis of isopropyl acetate by immobilized bacillus cereus lipase in organic medium. | selective production of fragrance fatty acid ester from isopropanol and acetic acid has been achieved using silica-immobilized lipase of bacillus cereus mtcc 8372. a purified thermoalkalophilic extracellular lipase was immobilized by adsorption onto the silica. the effects of various parameters like molar ratio of substrates (isopropanol and acetic acid; 25 to 100 mm), concentration of biocatalyst (25-125 mg/ml), reaction time, reaction temperature, organic solvents, molecular sieves, and initia ... | 2011 | 21603222 |
| screening of australian plants for antimicrobial activity against campylobacter jejuni. | campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. in this study, 115 extracts from 109 australian plant species were investigated for their antimicrobial activities against two c. jejuni strains using an in vitro broth microdilution assay. among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/ml against at least one c. jejuni strain. seventeen plant extracts were ... | 2011 | 21604309 |
| [screening endophytic bacteria against plant-parasitic nematodes]. | plant-parasite nematode is one of the most important pathogens in plant. our objective is to screen endophytic bacteria against plant-parasitic nematodes from plant. | 2011 | 21604551 |
| antibacterial activity of chemically defined chitosans: influence of molecular weight, degree of acetylation and test organism. | chitosans, polysaccharides obtained from the exoskeleton of crustaceans, have been shown to exert antibacterial activity in vitro and their use as a food preservative is of growing interest. however, beyond a consensus that chitosan appears to disrupt the bacterial cell membrane, published data are inconsistent on the chemical characteristics that confer the antibacterial activity of chitosan. while most authors agree that the net charge density of the polymer (reflected in the fraction of posit ... | 2011 | 21605923 |
| efficacy of vitrectomy in improving the outcome of bacillus cereus endophthalmitis. | purpose:: to evaluate the efficacy of vitrectomy with vancomycin for the treatment of experimental bacillus cereus endophthalmitis. methods:: endophthalmitis was initiated in rabbits via intravitreal injection of 100 colony-forming unit b. cereus. treatment groups included 25-gauge transconjunctival sutureless vitrectomy with intravitreal vancomycin (1 mg) or vancomycin alone. groups were treated at 4, 5, or 6 hours after infection. at 48 hours (for 4-hour and 5-hour groups) or 36 hours (for the ... | 2011 | 21606892 |
| mutually exclusive distribution of the sap and eag s-layer genes and the lytb/lyta cell wall hydrolase genes in bacillus thuringiensis. | recently, two bacillus thuringiensis strains were reported to synthesize parasporal inclusion bodies made not of the expected crystal (cry) proteins but rather of the surface layer proteins (slp) sap (encoded by sap) and ea1 (encoded by eag), respectively. whether the presence of the sap and eag genes is restricted to these two b. thuringiensis strains or ubiquitous in b. thuringiensis is unknown. we report here the distribution of the sap and eag genes in b. thuringiensis. strains in the bacill ... | 2011 | 21611767 |
| bacterial load of fresh vegetables and their resistance to the currently used antibiotics in saudi arabia. | abstract this study was carried out to describe the bacterial load and the occurrence of some disease-causing enteric bacteria on raw vegetables sold in saudi markets. the study further aimed to analyze antibiotic resistance rates, production of extended-spectrum beta lactamase, and plasmid carriage among bacterial population of raw vegetables. results revealed that none of them contained bacillus cereus, salmonella, and escherichia coli o157:h7. however, staphylococcus aureus and shigella were ... | 2011 | 21612423 |
| antifungal quinazolinones from marine-derived bacillus cereus and their preparation. | two new quinazolinones alkaloids, r(+)-2-(heptan-3-yl)quinazolin-4(3h)-one (1) and (2r,3'r)+(2s,3'r)-2-(heptan-3-yl)-2,3-dihydroquinazolin-4(1h)-one (2) (a pair of epimers), as well as seven known analogues, 2-methylquinazolin-4(3h)-one (3), 2-benzylquinazolin-4(3h)-one (4), cyclo-(pro-ile), cyclo-(pro-leu), cyclo-(pro-val), cyclo-(pro-phe), and cyclo-(tyr-pro) were isolated from the n-butyl alcohol extract of the marine-derived bacterium bacillus cereus 041381. the new compounds were identified ... | 2011 | 21612927 |
| some like it hot: the effects of climate change on reproduction, immune function and disease resistance in the cricket gryllus texensis. | in many parts of the world, climate change is increasing the frequency and severity of heat waves. how do heat waves impact short-lived poikilotherms such as insects? in the cricket, gryllus texensis, 6 days of elevated temperatures (i.e. 7°c above the average field temperature and 5°c above their preferred temperature) resulted in increased egg laying, faster egg development and greater mass gain. the increased temperature also increased activity of phenoloxidase and lysozyme-like enzymes, two ... | 2011 | 21613515 |
| natural resistance of rose petals to microbial attack. | petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ... | 2011 | 21614712 |
| preparation of mucoadhesive oral patches containing tetracycline hydrochloride and carvacrol for treatment of local mouth bacterial infections and candidiasis. | the specific aim of this work was to prepare mucoadhesive patches containing tetracycline hydrochloride and carvacrol in an attempt to develop a novel oral drug delivery system for the treatment of mouth infections. the bilayered patches were prepared using ethyl cellulose as a backing layer and carbopol 934 as a matrix mucoadhesive layer. patches were prepared with different loading amounts of tetracycline hydrochloride and carvacrol. the antimicrobial activity was assessed for the prepared pat ... | 2011 | 21617783 |
| molecular weight change of polyhydroxyalkanoate (pha) caused by the phac subunit of pha synthase from bacillus cereus yb-4 in recombinant escherichia coli. | polyhydroxyalkanoate (pha)-producing bacillus strains possess class iv pha synthases composed of two subunit types, namely, phar and phac. in the present study, pha synthases from bacillus megaterium nbrc15308(t) (pharc(bm)), b. cereus yb-4 (pharc(yb4)), and hybrids (phar(bm)c(yb4) and phar(yb4)c(bm)) were expressed in escherichia coli jm109 to characterize the molecular weight of the synthesized poly(3-hydroxybutyrate) [p(3hb)]. pharc(bm) synthesized p(3hb) with a relatively high molecular weig ... | 2011 | 21618968 |
| reference genes for quantitative, reverse-transcription pcr in bacillus cereus group strains throughout the bacterial life cycle. | quantitative reverse-transcription pcr (rt-qpcr) has become a major tool to better understand the biology and pathogenesis of bacteria. one prerequisite of valid rt-qpcr data is their proper normalization to stably expressed reference genes. to identify and evaluate reference genes suitable for normalization of gene expression data in bacillus cereus group strains, mrna levels of eleven candidate reference genes (rpsu, nifu, udp (udp-n-acetylglucosamine 2-epimerase), bt9727_5154/bc_5475, bt9727_ ... | 2011 | 21620905 |
| role played by exosporium glycoproteins in the surface properties of bacillus cereus spores and in their adhesion properties to stainless steel. | b. cereus spores are surrounded by a loose fitting layer, called the exosporium, whose distal part is mainly formed of glycoproteins. the role played by the exosporium glycoproteins of b. cereus atcc 14579 (bcla, exsh) was investigated considering hydrophobicity and charge, as well as adhesion properties of spores to stainless steel. the absence of bcla increased both the isoelectric point (iep) and hydrophobicity of whole spores, whilst simultaneously reducing the interaction between spores and ... | 2011 | 21622795 |
| phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth. | a local herb, kacip fatimah, is famous amongst malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of labisia pumila benth. total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. leaves of var. pumi ... | 2011 | 21623314 |
| examining the genetic variation of reference microbial cultures used within food and environmental laboratories using fluorescent amplified fragment length polymorphism analysis. | fluorescent amplified fragment length polymorphism (faflp) analysis was applied to genetically fingerprint 'working culture control strains' used by accredited food microbiology laboratories. a working culture control strain is defined as a sub-culture from a strain initially obtained from an authenticated source (such as the national collection of type cultures, nctc), that is maintained for use with routine testing within the laboratory. working culture control strains from eight food examinat ... | 2011 | 21623896 |
| physiological traits of the symbiotic bacterium teredinibacter turnerae isolated from the mangrove shipworm neoteredo reynei. | nutrition in the teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the teredinibacter clade. the mangrove teredinidae neoteredo reynei is popularly used in the treatment of infectious diseases in the north of brazil. in the present work, the symbionts of n. reynei, which are strictly confined to the host's gills, were conclusively identified as teredinibacter turnerae. symbiont variants obtained in vitro were able to grow using casein a ... | 2009 | 21637522 |
| automated spectral smoothing with spatially adaptive penalized least squares. | a variety of data smoothing techniques exist to address the issue of noise in spectroscopic data. the vast majority, however, require parameter specification by a knowledgeable user, which is typically accomplished by trial and error. in most situations, optimized parameters represent a compromise between noise reduction and signal preservation. in this work, we demonstrate a nonparametric regression approach to spectral smoothing using a spatially adaptive penalized least squares (sapls) approa ... | 2011 | 21639989 |
| bacillus anthracis: molecular taxonomy, population genetics, phylogeny and patho-evolution. | bacillus anthracis, the etiological agent of anthrax, manifests a particular bimodal lifestyle. this bacterial species alternates between short replication phases of 20-40 generations that strictly require infection of the host, normally causing death, interrupted by relatively long, mostly dormant phases as spores in the environment. hence, the b. anthracis genome is highly homogeneous. this feature and the fact that strains from nearly all parts of the world have been analysed for canonical si ... | 2011 | 21640849 |
| global gene expression profile of swarming bacillus cereus. | bacillus cereus can use swarming to move over and colonize solid surfaces in different environments. this kind of motility is a collective behavior accompanied by the production of long and hyperflagellate swarm cells. in this study, the genome-wide transcriptional response of b. cereus atcc 14579 during swarming was analyzed. swarming was shown to trigger the differential expression (>2 fold change) of 118 genes. down-regulated genes included those required for basic cellular metabolism. in acc ... | 2011 | 21642396 |
| lysozyme as a barrier to growth of bacillus anthracis strain sterne in liquid egg white, milk and beef. | in this study, we investigated the role of lysozyme on the viability of bacillus cereus, bacillus subtilis, bacillus pumilus and bacillus anthracis (sterne) in egg white (ew), ground beef and milk. at 35 °c in ew, growth rates (gr) for b. cereus, b. subtilis, b. pumilus and b. anthracis were 0.005, -0.018, -0.028 and -0.029 od(600)/h, respectively. heat-treating ew at 55 and 60 °c reduced the inactivating effect of ew by 3.1 and 10.5-fold, respectively. addition of lysozyme (2 mg/ml) to 60 °c-tr ... | 2011 | 21645824 |
| aerobic degradation of 2,4-dinitrotoluene by individual bacterial strains and defined mixed population in submerged cultures. | the degradation efficiencies of isomeric mononitrotoluenes (2- and 4-nts) and dinitrotoluenes (2,4-dnt and 2,6-dnt) by either individual bacterial strains (bacillus cereus ndt4, pseudomonas putida ndt1, pseudomonas fluorescens ndt2, and achromobacter sp. ndt3) or their mixture were compared in submerged batch cultivations. the mixed culture degraded 2,4-dnt nearly 50 times faster than any of the individual strains. the mixed culture also demonstrated significantly shorter lag periods in 2,4-dnt ... | 2011 | 21665364 |
| anti-proliferative activity of meroditerpenoids isolated from the brown alga stypopodium flabelliforme against several cancer cell lines. | the sea constitutes one of the most promising sources of novel compounds with potential application in human therapeutics. in particular, algae have proved to be an interesting source of new bioactive compounds. in this work, six meroditerpenoids (epitaondiol, epitaondiol diacetate, epitaondiol monoacetate, stypotriol triacetate, 14-ketostypodiol diacetate and stypodiol) isolated from the brown alga stypopodium flabelliforme were tested for their cell proliferation inhibitory activity in five ce ... | 2011 | 21673894 |
| dnaj sequences of bacillus cereus strains isolated from outbreaks of hospital infection are highly similar to bacillus anthracis. | bacillus cereus is becoming an important nomosomial pathogen because of frequent isolation from blood cultures and from severe systemic infections. to differentiate highly pathogenic outbreak strain of b. cereus from other sources of the bacillus cereus, we attempted to analyze their dnaj sequences. assays indicated that dnaj sequence similarity of all of 52 blood culture isolates of b. cereus ranged from 92.8% to 100%. the distance between b. anthracis and b. cereus except six outbreak isolates ... | 2011 | 21683265 |
| molecular phylogenetic diversity of bacillus community and its temporal-spatial distribution during the swine manure of composting. | in order to obtain the diversity and temporal-spatial distribution of bacillus community during the swine manure composting, we utilized traditional culture methods and the modern molecular biology techniques of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and -denaturing gradient gel electrophoresis (pcr-dgge). bacillus species were firstly isolated from the composting. based on temperature changes, the temporal-spatial characteristics of total culturable bacill ... | 2011 | 21701982 |
| comparing nonsynergy gamma models and interaction models to predict growth of emetic bacillus cereus for combinations of ph and water activity values. | this research aims to test the absence (gamma hypothesis) or occurrence of synergy between two growth-limiting factors, i.e., ph and water activity (a(w)), using a systematic approach for model selection. in this approach, preset criteria were used to evaluate the performance of models. such a systematic approach is required to be confident in the correctness of the individual components of the combined (synergy) models. with bacillus cereus f4810/72 as the test organism, estimated growth bounda ... | 2011 | 21705525 |
| role of the five rna helicases in the adaptive response of bacillus cereus atcc 14579 cells to temperature, ph, and oxidative stresses. | in this study, growth rates and lag times of the five rna helicase-deleted mutants of bacillus cereus atcc 14579 were compared to those of the wild-type strain under thermal, oxidative, and ph stresses. deletion of cshd and cshe had no impact under any of the tested conditions. deletion of csha, cshb, and cshc abolished growth at 12┬░c, confirming previous results. in addition, we found that each rna helicase had a role in a specific temperature range: deletion of csha reduced growth at all the ... | 2011 | 21705526 |
| bovine bacillus anthracis in cameroon. | bovine bacillus anthracis isolates from cameroon were genetically characterized. they showed a strong homogeneity, and they belong, together with strains from chad, to cluster a+¦, which appears to be predominant in western africa. however, one strain that belongs to a newly defined clade (d) and cluster (d1) is penicillin resistant and shows certain phenotypes typical of bacillus cereus. | 2011 | 21705535 |
| Biochemical and Mutational Studies of the Bacillus cereus CECT 5050T Formamidase Support the Existence of a C-E-E-K Tetrad in Several Members of the Nitrilase Superfamily. | Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate in the nitrogen metabolic pathway. Sequence analysis has revealed that at least two different groups of formamidases are classified as EC 3.5.1.49: on the one hand, the derivatives of the FmdA-AmdA superfamily, which are the best ... | 2011 | 21705545 |
| detection, cellular localization and antibacterial activity of two lytic enzymes of pediococcus acidilactici atcc 8042. | aim:ôçé in pediococcus acidilactici atcc 8042, two activities of peptidoglycan hydrolase (pgh) with lytic effect against micrococcus lysodeikticus and staphylococcus aureus have been detected. this work intends to elucidate the growth phase of maximum lytic activity, the localization and the effectiveness of the activity against pathogenic gram-negative and gram-positive bacteria. methods and results:ôçé cells were grown in mrs medium and collected at different growth stages, and the proteins we ... | 2011 | 21707881 |
| molecular characterization of bacillus cereus toxigenic strains isolated from different food matrices in jordan. | bacillus cereus is increasingly recognized as one of the causative agents of food poisoning in different parts of the world. | 2011 | 21714637 |
| compact dry(r) x-bc for the enumeration of bacillus cereus in food samples. | we evaluated the effectiveness of using compact dry(r) x-bc (cd-xbc), a ready-to-use and self-diffusing dry medium sheet culture system based on a novel detection principle, for the detection and enumeration of bacillus cereus. all 13 b. cereus strains, which were studied for the inclusivity study, grew as blue/green colonies on the cd-xbc. when 3 yeast strains and 103 bacterial strains other than b. cereus were tested for the exclusivity study, 5 strains formed white colonies, and 4 strains for ... | 2011 | 21719993 |
| hygienic quality and antibiotic resistance profile of sliced butchery. | in order to investigate the microbiological quality of different meat products on the greek market, 200 samples were collected from the following preparations: boiled turkey (n=50), boiled pork ham (n=50), smoked turkey (n=50) and smoked pork ham (n=50). in all cold meat preparations clostridium perfringens vegetative and spore forms, staphylococcus aureus, escherichia coli and other clostridium sp lec(-), as well as lactobacillus, bacillus sp. and salmonella sp. were recovered. for instance bac ... | 2011 | 21722746 |
| validation of the intact zwittermicin a biosynthetic gene cluster and discovery of a complementary resistance mechanism in bacillus thuringiensis. | zwittermicin a (zma) is a hybrid polyketide-nonribosomal peptide produced by certain bacillus cereus group strains. it displays broad-spectrum antimicrobial activity. its biosynthetic pathway in b. cereus has been proposed through analysis of the nonribosomal peptide synthetase (nrps) and polyketide synthase (pks) modules involved in zma biosynthesis. in this study, we constructed a bacterial artificial chromosome (bac) library from bacillus thuringiensis subsp. kurstaki strain ybt-1520 genomic ... | 2011 | 21730118 |
| selection of phytotoxin producing rhizobacteria. | in order to select phytotoxin producing rhizobacteria to control weed plants, twenty five bacterial strains previously isolated from the rhizospheres of various plants were grown in a liquid medium and, after cell removal by centrifugation, the liquid phases were freeze-dried and the products were extracted with ethyl acetate/methanol. the extracts were concentrated to dryness under vacuum and dissolved in water and sucrose solution to be submitted to in vitro assays of lettuce (lactuca sativa l ... | 2011 | 21739082 |
| a new class of 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazine analogues with antimicrobial/antimycobacterial activity. | this study presents the synthesis and in vitro pharmacological evaluations of novel 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazines. the title compounds were assayed for their in vitro antimicrobial activity against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria) and four fungi (aspergillus niger, aspergillus fumigatus, a ... | 2011 | 21740100 |
| molecular characterization of the probiotic strain bacillus cereus var. toyoi ncimb 40112 and differentiation from food poisoning strains. | bacillus cereus var. toyoi strain ncimb 40112 (toyocerin), a probiotic authorized in the european union as feed additive for swine, bovines, poultry, and rabbits, was characterized by dna fingerprinting applying pulsed-field gel electrophoresis and multilocus sequence typing and was compared with reference strains (of clinical and environmental origins). the probiotic strain was clearly characterized by pulsed-field gel electrophoresis using the restriction enzymes apa i and sma i resulting in u ... | 2011 | 21740723 |
| effects of physical culture parameters on bacteriocin production by mexican strains of bacillus thuringiensis after cellular induction. | we have shown previously that in the presence of inducer bacillus cereus 183, significant increases in bacteriocin production and bactericidal activity of b. thuringiensis occur when the latter is cultivated at ph 7.2, 28°c, and 180 rpm. here we show that this activity can be further improved when b. thuringiensis is induced with b. cereus 183 and then cultivated with modification of ph, temperature, and agitation. five native strains of b. thuringiensis, lbit 269, lbit 287, lbit 404, lbit 420, ... | 2011 | 21743998 |