Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| fine mapping and identification of candidate rice genes associated with qstv11(sg), a major qtl for rice stripe disease resistance. | rice stripe disease, caused by rice stripe virus (rsv) is a serious constraint to rice production in subtropical regions of east asia. we performed fine mapping of a rsv resistance qtl on chromosome 11, qstv11 ( sg ), using near-isogenic lines (nils, bc(6)f(4)) derived from a cross between the highly resistant variety, shingwang, and the highly susceptible variety, ilpum, using 11 insertion and deletion (indel) markers. qstv11 ( sg ) was localized to a 150-kb region between indel 11 (17.86 mbp) ... | 2012 | 22751999 |
| epidemiology, aetiology and management of childhood acute community-acquired pneumonia in developing countries--a review. | childhood acute community-acquired pneumonia is one of the leading causes of morbidity and mortality in developing countries. in children who have not received prior antibiotic therapy, the main bacterial causes of clinical pneumonia in developing countries are streptococcus pneumoniae and haemophilus influenzae type b (hib), and the main viral cause is respiratory syncytial virus (rsv), but estimates of their relative importance vary in different settings. the only vaccines for the prevention o ... | 2011 | 22783679 |
| [acute bronchiolitis: a prospective study]. | bronchiolitis is the most common lower respiratory infection in children under 2 years old. respiratory syncytial virus (rsv) is the most frequently involved etiologic agent. | 2011 | 22849929 |
| pathological observations of lung inflammation after administration of ip-10 in influenza virus- and respiratory syncytial virus-infected mice. | pneumonia is a common complication of influenza virus infection and a common cause of death of patients. the aim of this study was to test the hypothesis that interferon-γ-inducible protein-10 (ip-10) is an important chemokine in the development of airway inflammation caused by certain viruses. mice were infected with influenza virus after administration of murine ip-10 and the severity of pneumonia was compared with the group which was infected with influenza virus alone. another mice group was ... | 2011 | 22969848 |
| population diversity of rice stripe virus-derived sirnas in three different hosts and rnai-based antiviral immunity in laodelphgax striatellus. | small rna-mediated gene silencing plays evolutionarily conserved roles in gene regulation and defense against invasive nucleic acids. virus-derived small interfering rnas (vsirnas) are one of the key elements involved in rna silencing-based antiviral activities in plant and insect. vsirnas produced after viruses infecting hosts from a single kingdom (i.e., plant or animal) are well described. in contrast, vsirnas derived from viruses capable of infecting both plants and their insect vectors have ... | 2012 | 23029445 |
| identification of novel oryza sativa mirnas in deep sequencing-based small rna libraries of rice infected with rice stripe virus. | micrornas (mirnas) play essential regulatory roles in the development of eukaryotes. methods based on deep-sequencing have provided a powerful high-throughput strategy for identifying novel mirnas and have previously been used to identify over 100 novel mirnas from rice. most of these reports are related to studies of rice development, tissue differentiation, or abiotic stress, but novel rice mirnas related to viral infection have rarely been identified. in previous work, we constructed and pyro ... | 2012 | 23071571 |
| strong resistance against rice grassy stunt virus is induced in transgenic rice plants expressing double-stranded rna of the viral genes for nucleocapsid or movement proteins as targets for rna interference. | rice grassy stunt virus (rgsv), a member of the genus tenuivirus, causes significant economic losses in rice production in south, southeast, and east asian countries. growing resistant varieties is the most efficient method to control rgsv; however, suitable resistance genes have not yet been found in natural rice resources. one of the most promising methods to confer resistance against rgsv is the use of rna interference (rnai). it is important to target viral genes that play important roles in ... | 2013 | 23190115 |
| role of rice stripe virus nsvc4 in cell-to-cell movement and symptom development in nicotiana benthamiana. | our previous work has demonstrated that the nsvc4 protein of rice stripe virus (rsv) functions as a cell-to-cell movement protein. however, the mechanisms whereby rsv traffics through plasmodesmata (pd) are unknown. here we provide evidence that the nsvc4 moves on the actin filament and endoplasmic reticulum network, but not microtubules, to reach cell wall pd. disruption of cytoskeleton using different inhibitors altered nsvc4 localization to pd, thus impeding rsv infection of nicotiana bentham ... | 2012 | 23233857 |
| wheezing in infancy. | several population-based birth cohort studies documented that 30% of children suffer from wheezing during respiratory infections before their third birthday. infants are prone to wheeze because of anatomic factors related to the lung and chest wall in addition to immunologic and molecular influences in comparison to older children. viral infections lead to immunologic derangements that cause wheezing both in immunocompetent and immunodeficient infants. anatomic causes of wheeze may be extrinsic ... | 2011 | 23282443 |
| [prophylaxis against respiratory viral disease in pediatric and adult patients undergoing solid organ and hematopoietic stem cells transplantation]. | respiratory viruses have been identified as a cause of morbidity and mortality in patients undergoing sot and hsct, specially in children. the most frequent are respiratory syncytial virus (rsv), influenza (flu), parainfluenza (pi) and adenovirus (adv). these infections are associated with progression to severe lower respiratory tract infections in up to 60% of the cases. it is advised to apply universal protection recommendations for respiratory viruses (a2) and some specific measures for flu a ... | 2012 | 23282554 |
| combination therapy using monoclonal antibodies against respiratory syncytial virus (rsv) g glycoprotein protects from rsv disease in balb/c mice. | therapeutic options to control respiratory syncytial virus (rsv) are limited, thus development of new therapeutics is high priority. previous studies with a monoclonal antibody (mab) reactive to an epitope proximal to the central conserved region (ccr) of rsv g protein (mab 131-2g) showed therapeutic efficacy for reducing pulmonary inflammation rsv infection in balb/c mice. here, we show a protective effect in rsv-infected mice therapeutically treated with a mab (130-6d) reactive to an epitope w ... | 2012 | 23300550 |
| the incidence and clinical burden of respiratory syncytial virus disease identified through hospital outpatient presentations in kenyan children. | there is little information that describe the burden of respiratory syncytial virus (rsv) associated disease in the tropical african outpatient setting. | 2012 | 23300695 |
| [viruses and clinical features associated with hospitalized children with acute respiratory infections in lhasa, tibet]. | to investigate the viral etiology and clinical features of hospitalized children with acute respiratory tract infections in tibet. | 2012 | 23302560 |
| value of chest radiographic pattern in rsv disease of the newborn: a multicenter retrospective cohort study. | respiratory syncytial virus (rsv) lower respiratory tract infection is the most common viral respiratory infection in infants. several authors have sought to determine which risk factors are the best predictors for severe rsv disease. our aim was to evaluate if a specific chest radiographic pattern in rsv disease can predict the disease severity. we conducted a multicenter retrospective cohort study in term and preterm neonates with confirmed lower respiratory tract rsv infection, admitted to ne ... | 2012 | 23304470 |
| respiratory syncytial virus persistence in macrophages alters the profile of cellular gene expression. | viruses can persistently infect differentiated cells through regulation of expression of both their own genes and those of the host cell, thereby evading detection by the host’s immune system and achieving residence in a non-lytic state. models in vitro with cell lines are useful tools in understanding the mechanisms associated with the establishment of viral persistence. in particular, a model to study respiratory syncytial virus (rsv) persistence in a murine macrophage-like cell line has been ... | 2012 | 23342359 |
| the pneumonia virus of mice (pvm) model of acute respiratory infection. | pneumonia virus of mice (pvm) is related to the human and bovine respiratory syncytial virus (rsv) pathogens, and has been used to study respiratory virus replication and the ensuing inflammatory response as a component of a natural host—pathogen relationship. as such, pvm infection in mice reproduces many of the clinical and pathologic features of the more severe forms of rsv infection in human infants. here we review some of the most recent findings on the basic biology of pvm infection and it ... | 2012 | 23342367 |
| neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants. | respiratory syncytial virus (rsv) is the most common viral cause of childhood acute lower respiratory tract infections. it is estimated that rsv infections result in more than 100,000 deaths annually worldwide. bovine rsv is a cause of enzootic pneumonia in young dairy calves and summer pneumonia in nursing beef calves. furthermore, bovine rsv plays a significant role in bovine respiratory disease complex, the most prevalent cause of morbidity and mortality among feedlot cattle. infection of cal ... | 2012 | 23342375 |
| [prevalence of h1n1 a influenza virus infection among hospitalized patients with bronchiolitis twelve months old and younger]. | many pathogens have been reported to ause bronchiolitis during the winter season including he respiratory syncytial virus (rsv), influenza, oetapneumovirus, etc. the last h1n1 a influenza pandemic ccurred during the respiratory syncytial virus (rsv) eason. the role of the pandemic influenza strain as a ause of bronchiolitis was not documented. | 2012 | 23350292 |
| respiratory syncytial virus-associated hospitalizations in louisiana. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis and pneumonia in children under one year of age worldwide. records indicative of rsv cases were pulled from the louisiana inpatient hospital discharge data based on rsv diagnosis codes to describe the burden of rsv infections in louisiana from 1999 to 2010. two thousand to three thousand hospitalized rsv cases occurred each year, with rates ranging from 37.2 to 71.4 hospitalizations per 100,000 population and the majorit ... | 2012 | 23362592 |
| [differences of the regulation on the expression of mucin 1 induced by two single-strand rna viruses, respiratory syncytial virus and influenza virus]. | to investigate whether influenza virus (ifz) could up-regulate the expression of mucin 1 (muc1) which exists in epithelial cells of upper respiratory track to restrict the inflammation, as respiratory syncytial virus (rsv) does. quantitative rt-pcr and western blot were performed to detect the expression level of muc1 induced by two single-strand rna viruses in a549 cell lines. hep-2 and mdck cells were used respectively to culture rsv and ifz. at 24h post a549 cells infection with the same tite ... | 2012 | 23367563 |
| use of rapid human respiratory syncytial virus strip tests for detection of bovine respiratory syncytial virus in experimentally vaccinated calves. | three different rapid strip tests: tru rsv, binaxnow rsv and rsv respi-strip were compared with rt-pcr and elisa brsv ag for the ability to detect bovine respiratory syncytial virus (brsv) in nasal swabs collected from calves experimentally vaccinated with live vaccine rispoval rs-pi3. the reference strains of brsv (375 and a51908) were detected by elisa brsv ag whereas the strains of human respiratory syncytial virus (hrsv) and bovine parainfluenza virus type 3 (bpiv-3) were not. all rapid stri ... | 2012 | 23390751 |
| immunogenicity and protective efficacy of a dual subunit vaccine against respiratory syncytial virus and influenza virus. | respiratory syncytial virus (rsv) and influenza virus are the most significant pathogens causing respiratory tract diseases. composite vaccines are useful in reducing the number of vaccination and confer protection against multiple infectious agents. in this study, we generated fusion of rsv g protein core fragment (amino acid residues 131 to 230) and influenza ha1 globular head domain (amino acid residues 62 to 284) as a dual vaccine candidate. this fusion protein, gcf-ha1, was bacterially expr ... | 2012 | 23396871 |
| an insect cell line derived from the small brown planthopper supports replication of rice stripe virus, a tenuivirus. | a cell line from the small brown planthopper (sbph; laodelphax striatellus) was established to study replication of rice stripe virus (rsv), a tenuivirus. the sbph cell line, which had been subcultured through 30 passages, formed monolayers of epithelial-like cells. inoculation of cultured vector cells with rsv resulted in a persistent infection. during viral infection in the sbph cell line, the viral non-structural protein ns3 co-localized with the filamentous ribonucleoprotein particles of rsv ... | 2013 | 23468422 |
| regulation of tight junctions in upper airway epithelium. | the mucosal barrier of the upper respiratory tract including the nasal cavity, which is the first site of exposure to inhaled antigens, plays an important role in host defense in terms of innate immunity and is regulated in large part by tight junctions of epithelial cells. tight junction molecules are expressed in both m cells and dendritic cells as well as epithelial cells of upper airway. various antigens are sampled, transported, and released to lymphocytes through the cells in nasal mucosa ... | 2012 | 23509817 |
| enhanced early expression of membrane receptors with the rous sarcoma virus promoter in baculovirus-infected insect cells. | the effect of the rous sarcoma virus (rsv) long terminal repeat enhancer/promoter on expression levels of complementary dnas (cdnas) encoding seven transmembrane receptors was studied using the baculovirus expression vector system. expression of the human α(2b)-adrenoceptor (ar) cdna under the control of the polyhedrin (pol) promoter produced up to 7.6 pmol/mg protein at 28 h post infection (p.i.) in sf9 cells. the addition of the rsv promoter increased the expression to 11.6 pmol/mg protein. dr ... | 2012 | 23586865 |
| detection of quantitative trait loci (qtls) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. | small brown planthopper (sbph) and rice stripe virus (rsv) disease transmitted by sbph cause serious damage to rice (oryza sativa l.) in china. in the present study, we screened 312 rice accessions for resistance to sbph. the indica variety, n22, is highly resistant to sbph. one hundred and eighty two recombinant inbred lines (rils) derived from a cross of n22 and the highly susceptible variety, ussr5, were used for quantitative trait locus (qtl) analysis of resistances to sbph and rsv. in a mod ... | 2013 | 23591851 |
| transcription of orfs on rna2 and rna4 of rice stripe virus terminate at an auccggau sequence that is conserved in the genus tenuivirus. | rice stripe virus, the type member of the genus tenuivirus, has four genomic rnas. rnas 2-4 have an ambisense coding strategy and the noncoding intergenic regions (irs) separating the two orfs are thought to function in termination of transcription. sequencing the 3'-untranslated region of transcripts from rna2 and rna4 in virus-infected oryza sativa (the natural host), nicotiana benthamiana (an experimental host) and laodelphax striatellus (the vector), showed that the sequences of p2 and pc2 t ... | 2013 | 23624227 |
| rice yellow stunt rhabdovirus protein 6 suppresses systemic rna silencing by blocking rdr6-mediated secondary sirna synthesis. | the p6 protein of rice yellow stunt rhabdovirus (rysv) is a virion structural protein that can be phosphorylated in vitro. however its exact function remains elusive. we found that p6 enhanced the virulence of potato virus x (pvx) in nicotiana benthamiana and n. tabacum plants, suggesting that it might function as a suppressor of rna silencing. we examined the mechanism of p6-mediated silencing suppression by transiently expressing p6 in both n. benthamiana leaves and rice protoplasts. our resul ... | 2013 | 23634838 |
| respiratory syncytial virus prophylaxis in preterm infants: a cost-effectiveness study from turkey. | the main aim of this study was to evaluate the cost-effectiveness of respiratory syncytial virus (rsv) prophylaxis with palivizumab in turkey, by comparing hospitalization rates and costs as well as results of risk analyses in preterm infants who were treated either with palivizumab or conservatively. this retrospective study was undertaken in two centers on infants born with a gestational age of ≤32 weeks during the 2010-2011 seasons. patients were divided into two groups based on status of rsv ... | 2012 | 23692714 |
| characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus. | replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ... | 2013 | 23776591 |
| one-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses. | rice stripe virus (rsv), rice black-streaked dwarf virus (rbsdv), and rice dwarf virus (rdv) are major rice-infecting viruses in korea that can cause serious crop losses. a one-step multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed for the simultaneous detection of these rice viruses. three sets of specific primers targeted to the capsid protein coding genes of rsv, rbsdv, and rdv were used to amplify fragments that were 703 bp, 485 bp, and 252 bp, respectively. t ... | 2013 | 23850700 |
| respiratory virus laboratory pandemic planning and surveillance in central viet nam, 2008-2010. | laboratory capacity is needed in central viet nam to provide early warning to public health authorities of respiratory outbreaks of importance to human health, for example the outbreak of influenza a(h1n1) pandemic in 2009. polymerase chain reaction (pcr) procedures established as part of a capacity-building process were used to conduct prospective respiratory surveillance in a region where few previous studies have been undertaken. | 2012 | 23908924 |
| production of marker-free and rsv-resistant transgenic rice using a twin t-dna system and rnai. | a twin t-dna system is a convenient strategy for creating selectable marker-free transgenic plants. the standard transformation plasmid, pcambia 1300, was modified into a binary vector consisting of two separate t-dnas, one of which contained the hygromycin phosphotransferase (hpt) marker gene. using this binary vector, we constructed two vectors that expressed inverted-repeat (ir) structures targeting the rice stripe virus (rsv) coat protein (cp) gene and the special-disease protein (sp) gene. ... | 2013 | 23938389 |
| genetic variability and evolution of rice stripe virus. | rice stripe virus (rsv) is the type member of the genus tenuivirus. rsv is known to have four segmented, single-stranded rna molecules and causes rice stripe disease in the rice fields of china, japan, and korea. based on the complete genomic sequences of the determined 6 rsv isolates (from yunnan, jiangsu, zhejiang, and liaoning provinces, china) and 27 other rsv isolates (from yunnan, jiangsu, anhui, henan, and shandong provinces of china, also japan and korea) downloaded from genbank, we prov ... | 2013 | 24101204 |
| investigation on subcellular localization of rice stripe virus in its vector small brown planthopper by electron microscopy. | rice stripe virus (rsv), which is transmitted by small brown planthopper (laodelphax striatellus fallén, sbph), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many east asian countries, including china. investigation on viral localization in the vector is very important for elucidating transmission mechanisms of rsv by sbph. in this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localiza ... | 2013 | 24139455 |
| interaction between rice stripe virus disease-specific protein and host psbp enhances virus symptoms. | rice stripe virus (rsv) causes severe diseases in oryza sativa (rice) in many eastern asian countries. disease-specific protein (sp) of rsv is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of rsv symptoms. here, we present evidence that expression of rsv sp alone in rice or nicotiana benthamiana did not produce visible symptoms. expression of sp in these two plants, however, enhanced rsv- or potato virus x (pvx)-induced symptoms. through ye ... | 2014 | 24214893 |
| construction of single-chain variable fragment antibodies against mcf-7 breast cancer cells. | a phage display library of single chain variable fragment (scfv) against mcf-7 breast cancer cells was constructed from c3a8 hybridoma cells. rna from the c3a8 was isolated, cdna was constructed, and variable heavy and light immunoglobulin chain gene region were amplified using pcr. the variable heavy and light chain gene regions were combined with flexible linker, linked to a pcantab 5e phagemid vector and electrophoresed into supe strain of escherichia coli tg1 cells. forty-eight clones demons ... | 2013 | 24301925 |
| migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia. | this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ... | 2013 | 24312081 |
| comparative transcriptome analysis of two rice varieties in response to rice stripe virus and small brown planthoppers during early interaction. | rice stripe, a virus disease, transmitted by a small brown planthopper (sbph), has greatly reduced production of japonica rice in east asia, especially in china. although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance. by de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to sbph and rsv infection in rice. respectively 648 and 937 degs were detect ... | 2013 | 24358146 |
| interaction study of rice stripe virus proteins reveals a region of the nucleocapsid protein (np) required for np self-interaction and nuclear localization. | rice stripe virus (rsv), which belongs to the genus tenuivirus, is an emergent virus problem. the rsv genome is composed of four single-strand rnas (rna1-rna4) and encodes seven proteins. we investigated interactions between six of the rsv proteins by yeast-two hybrid (y2h) assay in vitro and by bimolecular fluorescence complementation (bifc) in planta. y2h identified self-interaction of the nucleocapsid protein (np) and ns3, while bifc revealed self-interaction of np, ns3, and ncp. to identify ... | 2014 | 24463502 |
| nonstructural protein ns4 of rice stripe virus plays a critical role in viral spread in the body of vector insects. | rice stripe virus (rsv), a tenuivirus, is transmitted by small brown planthopper (sbph) in a persistent-propagative manner. in this study, sequential infection of rsv in the internal organs of sbph after ingestion of virus indicated that rsv initially infected the midgut epithelium, and then progressed to the visceral muscle tissues, through which rsv spread to the entire alimentary canal. finally, rsv spread into the salivary glands and reproductive system. during viral infection, the nonstruct ... | 2014 | 24523924 |
| transgenic rice expressing rice stripe virus ns3 protein, a suppressor of rna silencing, shows resistance to rice blast disease. | the ns3 protein of rice stripe virus (rsv), encoded by the virion strand of rna3, is a viral suppressor of rna silencing (vsr). rice expressing ns3 had a normal phenotype, was initially sensitive to rsv but recovered at the later stages of infection. rsv accumulated slightly more in transgenic than in wild-type plants at the early stage of infection, but accumulation was similar later. transgenic rice expressing ns3 also showed enhanced resistance to the fungus magnaporthe oryzae. meanwhile, exp ... | 2014 | 24557730 |
| a transmembrane domain determines the localization of rice stripe virus pc4 to plasmodesmata and is essential for its function as a movement protein. | the pc4 protein encoded by rice stripe virus (rsv) is a viral movement protein (mp). a transmembrane (tm) domain spanning aas 106-123 of pc4 was identified and shown to be essential for localization of pc4 to plasmodesmata (pd) (but not to chloroplasts) and for its ability to recover the movement of movement-deficient pvx. analysis of alanine-scanning mutants showed that m116a and g120a had a similar localization to wild type pc4, being localized at pd and chloroplasts, but all other mutants wer ... | 2014 | 24560843 |
| transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector. | most plant viruses are transmitted by hemipteroid insects. some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. rice stripe virus (rsv), a tenuivirus, transmitted mainly by the small brown planthopper (laodelphax striatellus), is also spread to the offspring through the eggs. here, we used the rsv-planthopper system as a model to investigate the mechanism of transovarial transmission and demonst ... | 2014 | 24603905 |
| heat shock protein 70 is necessary for rice stripe virus infection in plants. | heat shock proteins 70 (hsp70s) are a highly conserved family of genes in eukaryotes, and are involved in a remarkable variety of cellular processes. in many plant positive-stranded rna viruses, hsp70 participates in the construction of a viral replication complex and plays various roles during viral infection. here, we found increased expression of hsp70 following infection by rice stripe virus (rsv), a negative-stranded rna virus, in both rice (the natural host) and nicotiana benthamiana (an e ... | 2014 | 24823923 |
| stv11 encodes a sulphotransferase and confers durable resistance to rice stripe virus. | rice stripe virus (rsv) causes one of the most serious viral diseases of rice (oryza sativa l.), but the molecular basis of rsv resistance has remained elusive. here we show that the resistant allele of rice stv11 (stv11-r) encodes a sulfotransferase (ossot1) catalysing the conversion of salicylic acid (sa) into sulphonated sa (ssa), whereas the gene product encoded by the susceptible allele stv11-s loses this activity. sequence analyses suggest that the stv11-r and stv11-s alleles were prediffe ... | 2014 | 25203424 |
| current insights into research on rice stripe virus. | rice stripe virus (rsv) is one of the most destructive viruses of rice, and greatly reduces rice production in china, japan, and korea, where mostly japonica cultivars of rice are grown. rsv is transmitted by the small brown plant-hopper (sbph) in a persistent and circulative-propagative manner. several methods have been developed for detection of rsv, which is composed of four single-stranded rnas that encode seven proteins. genome sequence data and comparative phylogenetic analysis have been u ... | 2013 | 25288949 |
| transcriptome analysis of the small brown planthopper, laodelphax striatellus carrying rice stripe virus. | rice stripe virus (rsv), the type member of the genus tenuivirus, transmits by the feeding behavior of small brown planthopper (sbph), laodelphax striatellus. to investigate the interactions between the virus and vector insect, total rna was extracted from rsv-viruliferous sbph (rvls) and non-viruliferous sbph (nvls) adults to construct expressed sequence tag databases for comparative transcriptome analysis. over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of ... | 0 | 25288960 |
| rice stripe tenuivirus p2 may recruit or manipulate nucleolar functions through an interaction with fibrillarin to promote virus systemic movement. | rice stripe virus (rsv) is the type species of the genus tenuivirus and represents a major viral pathogen affecting rice production in east asia. in this study, rsv p2 was fused to yellow fluorescent protein (p2-yfp) and expressed in epidermal cells of nicotiana benthamiana. p2-yfp fluorescence was found to move to the nucleolus initially, but to leave the nucleolus for the cytoplasm forming numerous distinct bright spots there at later time points. a bimolecular fluorescence complementation (bi ... | 2015 | 25431002 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| alterations in sirna and mirna expression profiles detected by deep sequencing of transgenic rice with sirna-mediated viral resistance. | rna-mediated gene silencing has been demonstrated to serve as a defensive mechanism against viral pathogens by plants. it is known that specifically expressed endogenous sirnas and mirnas are involved in the self-defense process during viral infection. however, research has been rarely devoted to the endogenous sirna and mirna expression changes under viral infection if the resistance has already been genetically engineered in plants. aiming to gain a deeper understanding of the rna-mediated gen ... | 2015 | 25559820 |
| rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos. | plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ... | 2015 | 25601039 |
| rice stripe tenuivirus nonstructural protein 3 hijacks the 26s proteasome of the small brown planthopper via direct interaction with regulatory particle non-atpase subunit 3. | the ubiquitin/26s proteasome system plays a vital role in regulating host defenses against pathogens. previous studies have highlighted different roles for the ubiquitin/26s proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. in this study, we determined that the 26s proteasome is present in the small brown planthopper (sbph) (laodelphax striatellus) and has components similar to those in plants and ... | 2015 | 25653432 |
| first discovery of acetone extract from cottonseed oil sludge as a novel antiviral agent against plant viruses. | a novel acetone extract from cottonseed oil sludge was firstly discovered against plant viruses including tobacco mosaic virus (tmv), rice stripe virus (rsv) and southern rice black streaked dwarf virus (srbsdv). gossypol and β-sitosterol separated from the acetone extract were tested for their effects on anti-tmv and analysed by nuclear magnetic resonance (nmr) assay. in vivo and field trials in different geographic distributions and different host varieties declared that this extract mixture w ... | 2015 | 25705894 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| functional comparison of rna silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus. | rice grassy stunt virus (rgsv) is a member of the genus tenuivirus, which includes rice stripe virus (rsv), as the type species. a viral suppressor of rna silencing (vsr) of rgsv has not been identified, whereas the p3 protein of rsv (rsvp3) encoded by the viral-sense (v) strand of rna3 has been reported to act as a vsr. in this study, we examined the vsr function of the p5 protein of rgsv (rgsvp5), encoded by vrna5. expecting it to correspond to the vrna3 of rsv, we compared the vsr function of ... | 2015 | 25836276 |
| analysis of genetic variation and diversity of rice stripe virus populations through high-throughput sequencing. | plant rna viruses often generate diverse populations in their host plants through error-prone replication and recombination. recent studies on the genetic diversity of plant rna viruses in various host plants have provided valuable information about rna virus evolution and emergence of new diseases caused by rna viruses. we analyzed and compared the genetic diversity of rice stripe virus (rsv) populations in oryza sativa (a natural host of rsv) and compared it with that of the rsv populations ge ... | 2015 | 25852724 |
| [construction of rice stripe virus ns2 and ns3 co-rnai transgenic rice and disease-resistance analysis]. | ns2 and ns3 are two post-transcriptional gene silencing suppressors that are encoded by rice stripe virus. gene silencing suppressors are always related to the pathogenicity of viruses. in this study, the cdna of ns2 and ns3 were recombined by overlapping pcr assays, ligated to the rnai vector, and inserted into the pxq expression vector using pst i; the expressed vector was transferred into calluses induced from seeds of the japonica rice cultivar, 'nipponbare', using an agrobacterium-mediated ... | 2014 | 25868281 |
| [stress effects of imidacloprid on rsv in rice plants]. | the rice stripe disease is a viral disease transmitted by small brown planthopper, laodelphax striatellus, which outbroke a few years ago in the yangtze river basin, especially jiangsu region, china. to study the effects of imidacloprid stress on rice stripe virus (rsv) in rice plants, the rice seedlings were treated with imidacloprid 1, 2, 3 and 4 times (b1, b2, b3 and b4), respectively, after artificial inoculation by l. striatellus for 48 h, and the expression levels of relative genes includi ... | 2014 | 25876413 |
| proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein. | numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ... | 2015 | 26091699 |
| itraq-based quantitative proteomics analysis of rice leaves infected by rice stripe virus reveals several proteins involved in symptom formation. | rice plants infected by rice stripe virus (rsv) usually leads to chlorosis and death of newly emerged leaves. however, the mechanism of rsv-induced these symptoms was not clear. | 2015 | 26113023 |
| osrdr6 plays role in host defense against double-stranded rna virus, rice dwarf phytoreovirus. | rnai is a major antiviral defense response in plant and animal model systems. rna-dependent rna polymerase 6 (rdr6) is an essential component of rnai, which plays an important role in the resistance against viruses in the model plants. we found previously that rice rdr6 (osrdr6) functioned in the defense against rice stripe virus (rsv), and rice dwarf phytoreovirus (rdv) infection resulted in down-regulation of expression of rdr6. here we report our new findings on the function of osrdr6 against ... | 2015 | 26165755 |
| rice stripe virus counters reduced fecundity in its insect vector by modifying insect physiology, primary endosymbionts and feeding behavior. | virus-vector relationships can be complex and diverse as a result of long-term coevolution. understanding these interactions is crucial for disease and vector management. rice stripe virus (rsv) is known to be transovarially transmitted within its vector, laodelphax striatellus, and causes serious rice stripe disease. in rsv-infected l. striatellus, we found contrasting changes in vector fecundity, physiology, primary endosymbionts (i.e. yeast-like symbionts, yls) and feeding behavior that can i ... | 2015 | 26211618 |
| the rna-binding properties and domain of rice stripe virus nucleocapsid protein. | the nucleocapsid protein (np) of rice stripe virus (rsv) encapsidates viral genomic rnas to form virion. the binding of np with rna is essential for the formation of virus particle. in this study, the binding specificity of rsv np to rna and the domains within the np that mediate this interaction were investigated by gel electrophoretic mobility shift assays and northwestern blot analysis. the results demonstrated that rsv np was able to bind to all synthetic rnas and dnas without sequence speci ... | 2015 | 26250446 |
| time-course rna-seq analysis reveals transcriptional changes in rice plants triggered by rice stripe virus infection. | rice stripe virus (rsv) has become a major pathogen of rice. to determine how the rice transcriptome is modified in response to rsv infection, we used rna-seq to perform a genome-wide gene expression analysis of a susceptible rice cultivar. the transcriptomes of rsv-infected samples were compared to those of mock-treated samples at 3, 7, and 15 days post-infection (dpi). from 8 to 11% of the genes were differentially expressed (>2-fold difference in expression) in rsv-infected vs. noninfected ri ... | 2015 | 26305329 |
| interaction of hsp20 with a viral rdrp changes its sub-cellular localization and distribution pattern in plants. | small heat shock proteins (shsps) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. rice stripe virus (rsv) causes a severe disease of rice in eastern asia. oshsp20 and its homologue (nbhsp20) were used as baits in yeast two-hybrid (yth) assays to screen an rsv cdna library and were found to interact with the viral rna-dependent rna polymerase (rdrp) of rsv. interactions were confirmed by pull-dow ... | 2015 | 26359114 |
| rice responses and resistance to planthopper-borne viruses at transcriptomic and proteomic levels. | rice (oryza sativa) is one of the most important cereal crops in the world, especially in asian areas. rice virus diseases are considered as the most serious threat to rice yields. most rice viruses are transmitted by hemipteran insects such as planthoppers and leafhoppers. in asia five rice viruses are transmitted mainly by three planthopper species in a persistent manner: rice stripe virus, rice black-streaked dwarf virus, rice ragged stunt virus, rice grassy stunt virus, and southern rice bla ... | 2016 | 26363817 |
| identification and regulation of host genes related to rice stripe virus symptom production. | viral infections cause plant chlorosis, stunting, necrosis or other symptoms. the down-regulation of chloroplast-related genes (chrgs) is assumed to be responsible for chlorosis. we identified the differentially expressed genes (degs) in rice stripe virus (rsv)-infected nicotiana benthamiana, and examined the contribution of 75 down-regulated degs to rsv symptoms by silencing them one by one using tobacco rattle virus (trv)-induced gene silencing. silencing of 11 of the 75 down-regulated degs ca ... | 2016 | 26487490 |
| assessment of reference gene stability in rice stripe virus and rice black streaked dwarf virus infection rice by quantitative real-time pcr. | stably expressed reference gene(s) normalization is important for the understanding of gene expression patterns by quantitative real-time pcr (rt-qpcr), particularly for rice stripe virus (rsv) and rice black streaked dwarf virus (rbsdv) that caused seriously damage on rice plants in china and southeast asia. | 2015 | 26497487 |
| intracellular localization of rice stripe virus rna-dependent rna polymerase and its interaction with nucleocapsid protein. | the rna-dependent rna polymerase (rdrp) of rice stripe virus (rsv) is critical for both the transcription and replication of the viral genome. despite its importance, little is known about how it functions in cells. in the present study, rsv rdrp was split into three pieces, since expression of the full protein could not be achieved. then, the intracellular localization of these three rdrp fragments and their interactions with nucleocapsid protein (np) were investigated, which is another viral p ... | 2015 | 26560706 |
| different pathogenicities of rice stripe virus from the insect vector and from viruliferous plants. | persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. however, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. in this study, we used rice stripe virus (rsv), its insect vector laodelphax striatellus and host plant (oryza sativa) to explore how persistent viruses acquire pathogenicity from insect vectors. rsv acquired phytopathogenicity in both the alim ... | 2016 | 26585422 |
| organ-specific transcriptome response of the small brown planthopper toward rice stripe virus. | rice stripe virus (rsv) causes rice stripe disease and is transmitted by the small brown planthopper (laodelphax striatellus, sbph) in a persistent, circulative, and propagative manner. the alimentary canal and salivary gland of sbph play important roles in viral replication and transmission. however, little is known about the underlying molecular functions of these two organs in the interaction between rsv and sbph. in this study, organ-specific transcriptomes of the alimentary canal and saliva ... | 2016 | 26678499 |
| integrative analysis of the micrornaome and transcriptome illuminates the response of susceptible rice plants to rice stripe virus. | rice stripe virus (rsv) is one of the most serious rice viruses in east asia. to investigate how rice responds to rsv infection, we integrated mirna expression with parallel mrna transcription profiling by deep sequencing. a total of 570 mirnas were identified of which 69 mirnas (56 up-regulated and 13 down-regulated) were significantly modified by rsv infection. digital gene expression (dge) analysis showed that 1274 mrnas (431 up-regulated and 843 down-regulated genes) were differentially expr ... | 2016 | 26799317 |
| a signaling cascade from mir444 to rdr1 in rice antiviral rna silencing pathway. | plant rna-dependent rna polymerase1 (rdr1) is a key component of the antiviral rna-silencing pathway, contributing to the biogenesis of virus-derived small interfering rnas. this enzyme also is responsible for producing virus-activated endogenous small interfering rnas to stimulate the broad-spectrum antiviral activity through silencing host genes. the expression of rdr1 orthologs in various plants is usually induced by virus infection. however, the molecular mechanisms of activation of rdr1 exp ... | 2016 | 26858364 |
| viruliferous rate of small brown planthopper is a good indicator of rice stripe disease epidemics. | rice stripe virus (rsv), its vector insect (small brown planthopper, sbph) and climatic conditions in jiangsu, china were monitored between 2002 and 2012 to determine key biotic and abiotic factors driving epidemics of the disease. average disease severity, disease incidence and viruliferous rate of sbph peaked in 2004 and then gradually decreased. disease severity of rsv was positively correlated with viruliferous rate of the vector but not with the population density of the insect, suggesting ... | 2016 | 26898155 |
| mapping of the regions involved in self-interaction of rice stripe virus p3 protein. | rice stripe virus (rsv) protein p3 is a suppressor of rna silencing in plants. p3 has been shown by biomolecular fluorescence complementation assay to self-interact in planta but the regions responsible for homotypic interaction have not been determined. here we analyzed the domains for the self-interaction of p3 by using yeast two-hybrid, co-immunoprecipitation and fluorescence experiments. the results showed that p3 was also able to interact with itself in yeast and insect cells. the domain re ... | 2016 | 26982473 |
| roles of the laodelphax striatellus down syndrome cell adhesion molecule in rice stripe virus infection of its insect vector. | the arthropod down syndrome cell adhesion molecule (dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. to date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. in the present study, we cloned and characterized the cdna of laodelphax striatellus dscam (lsdscam) and investigated the function of lsdscam in rice stripe vir ... | 2016 | 26991800 |
| seasonal changes in the percentage of rice stripe virus viruliferous laodelphax striatellus (hemiptera: delphacidae) in paddy fields in japan. | rice stripe disease, which is caused by rice stripe virus (rsv), is one of the most serious viral diseases of rice. rsv is transmitted in a persistent manner bylaodelphax striatellus(fallén). the incidence of the disease can be estimated from the density of viruliferous vectors. understanding seasonal changes of the percentage of viruliferousl. striatelluscan facilitate forecasting and controlling the disease. in paddies, the percentage of viruliferous insects fluctuated in phase with the rate o ... | 2016 | 27099363 |
| differential proteomics profiling of the ova between healthy and rice stripe virus-infected female insects of laodelphax striatellus. | rice stripe virus-infected females of the small brown planthopper (sbph, laodelphax striatellus) usually lay fewer eggs with a longer hatch period, low hatchability, malformation and retarded or defective development compared with healthy females. to explore the molecular mechanism of those phenomena, we analyzed the differential proteomics profiling of the ova between viruliferous and healthy female insects using an isobaric tag for relative and absolute quantitation (itraq) approach. we obtain ... | 2016 | 27277140 |
| artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus. | rice stripe virus (rsv), the causative agent of rice stripe disease, is transmitted by laodelphax striatellus in a persistent-propagative manner. efficient virus acquisition is primary for studies of virus transmission and virus-insect vector interactions. however, under greenhouse conditions, less than 30% of the l. striatellus population, on average, become viruliferous during feeding on rsv-infected plants. here, we explored a method for efficient rsv acquisition by feeding the insects with a ... | 2016 | 27283882 |
| inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of rice stripe virus. | two tenuiviruses rice stripe virus (rsv) and rice grassy stunt virus (rgsv) were found to co-infect rice with the same reovirus rice ragged stunt virus (rrsv). during the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-rna leaders from the rrsv. a total of 245 and 102 rrsv-rgsv and rrsv-rsv chimeric mrna clones, respectively, were sequenced. an analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped lead ... | 2016 | 27393974 |
| overexpression of bsr1 confers broad-spectrum resistance against two bacterial diseases and two major fungal diseases in rice. | broad-spectrum disease resistance against two or more types of pathogen species is desirable for crop improvement. in rice, xanthomonas oryzae pv. oryzae (xoo), the causal bacteria of rice leaf blight, and magnaporthe oryzae, the fungal pathogen causing rice blast, are two of the most devastating pathogens. we identified the rice broad-spectrum resistance 1 (bsr1) gene for a bik1-like receptor-like cytoplasmic kinase using the fox hunting system, and demonstrated that bsr1-overexpressing (ox) ri ... | 2016 | 27436950 |
| time-course small rna profiling reveals rice mirnas and their target genes in response to rice stripe virus infection. | it has been known that many micrornas (mirnas) are involved in the regulation for the plant development and defense mechanism by regulating the expression of the target gene. several previous studies has demonstrated functional roles of mirnas in antiviral defense mechanisms. in this study, we employed high-throughput sequencing technology to identify rice mirnas upon rice stripe virus (rsv) infection at three different time points. six libraries from mock and rsv-infected samples were subjected ... | 2016 | 27626631 |
| rna-seq-based digital gene expression analysis reveals modification of host defense responses by rice stripe virus during disease symptom development in arabidopsis. | virus infection induces and suppresses host gene expression on a global level. rice stripe virus (rsv) is the type species of the genus tenuivirus and infects rice and arabidopsis plants. microarray-based and next generation sequencing-based transcriptomic approaches have been used to study rice-rsv interactions. however, our knowledge of the response of arabidopsis plants to rsv infection is limited, and it requires further investigation to determine the similarities (or differences) in virus-h ... | 2016 | 27912765 |
| rice stripe virus infection alters mrna levels of sphingolipid-metabolizing enzymes and sphingolipids content in laodelphax striatellus. | sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. here we report the identification of 12 sphingolipid genes and their regulation by rice stripe virus in the small brown planthopper (laodelphax striatellus fallén), a serious pest of rice throughout eastern asia. according to protein sequence similarity, we identified 12 sphingolipid enzyme gen ... | 2017 | 28130458 |
| evolution of rice stripe virus. | rice stripe virus (rsv) is an insect-borne tenuivirus of economical significance. it is endemic to the rice-growing regions of east asia and exhibits more genetic diversity in yunnan province of china. to gain more insights into the molecular epidemiology and evolution of rsv, recombination analyses were conducted and potential events were detected in each of the four rna segments of rsv. bayesian coalescent method was then applied to the time-stamped coding sequences of the cp gene. the nucleot ... | 2017 | 28189616 |
| identification of residues or motif(s) of the rice stripe virus ns3 protein required for self-interaction and for silencing suppressor activity. | rice stripe virus (rsv) is an important pathogen of rice. the rsv genome consists of four single-stranded rna segments that encode seven viral proteins. a previous report found that ns3 is a viral suppressor of rna silencing and self interacts. using a model that predicts protein structure, we identified amino acid residues or motifs, including four α-helix motifs, required for ns3 self-interaction. we then used yeast two-hybrid (y2h) and bimolecular fluorescence complementation (bifc) assays to ... | 2017 | 28392445 |
| development of a simplified rt-pcr without rna isolation for rapid detection of rna viruses in a single small brown planthopper (laodelphax striatellus fallén). | the small brown planthopper (sbph) is an important pest of cereal crops and acts as a transmission vector for multiple rna viruses. rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. reverse transcription polymerase chain reaction (rt-pcr) is a rapid, sensitive and reliable method for virus detection. the traditional rt-pcr contains a rna isolation step and is widely used for virus detection in insect. however, using the traditional rt-pcr for ... | 2017 | 28468626 |
| bacterial microbiota in small brown planthopper populations with different rice viruses. | the small brown planthopper (sbph) is an important virus vector, transmitting rice stripe virus (rsv), and rice black-streaked dwarf virus (rbsdv). insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by sbph. here, we investigated endosymbiont communities in non-viruliferous, rsv-infected, and rbsdv-infected sbph populations using illumina 16s rrna gene miseq sequencing. in total, 281,803 effective sequences ... | 2017 | 28471518 |
| quantitative analysis of rice stripe virus in a transovarial transmission cycle during the development and reproduction of its vector, laodelphax striatellus. | the amount of rice stripe virus (rsv) maintained through transovarial transmission was analyzed during the development and reproduction of its vector, laodelphax striatellus. reverse transcription quantitative pcr analysis was used to quantify rna expressed from the rsv coat protein (cp) gene as an estimate of rsv content in nymphs and adults of l. striatellus at various developmental stages. the 18s ribosome rna gene of l. striatellus was chosen as the reference for calculating rsv cp expressio ... | 2017 | 28589385 |
| the c-jun n-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication. | no evidence has shown whether insect-borne viruses manipulate the c-jun n-terminal kinase (jnk) signaling pathway of vector insects. using a system comprising the plant virus rice stripe virus (rsv) and its vector insect, the small brown planthopper, we have studied the response of the vector insect's jnk pathway to plant virus infection. we found that rsv increased the level of tumor necrosis factor-α and decreased the level of g protein pathway suppressor 2 (gps2) in the insect vector. the vir ... | 2017 | 28716183 |