Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| adaptive immunity and enhanced cd8+ t cell response to listeria monocytogenes in the absence of perforin and ifn-gamma. | single ag-specific cd8+ t cells from ifn-gamma-deficient (gko) or perforin-deficient (pko) mice provide substantial immunity against murine infection with listeria monocytogenes. to address the potential for redundancy between perforin and ifn-gamma as cd8+ t cell effector mechanisms, we generated perforin/ifn-gamma (pko/gko) double-deficient mice. pko/gko-derived cd8+ t cells specific for the immunodominant listeriolysin o (llo91-99) epitope provide immunity to lm infection similar to that prov ... | 2000 | 10843700 |
| restriction fragment differential display of pediocin-resistant listeria monocytogenes 412 mutants shows consistent overexpression of a putative beta-glucoside-specific pts system. | pediocin pa-1, which is a bacteriocin produced by lactic acid bacteria, has potential as a biopreservative of food. however, such use may lead to the development of resistance in the target organism. gene expression in two independent pediocin-resistant mutants of listeria monocytogenes 412 was compared to the original isolate by restriction fragment differential display pcr (rfdd-pcr). this method amplifies cdna restriction fragments under stringent pcr conditions, enabled by the use of specifi ... | 2000 | 10846216 |
| predictive models of the combined effects of curvaticin 13, nacl and ph on the behaviour of listeria monocytogenes atcc 15313 in broth. | thirty-three strains of listeria monocytogenes belonging to different serotypes were tested for their sensitivity to curvaticin 13, an antilisterial bacteriocin produced by lactobacillus curvatus sb13, using the well diffusion method in institut pasteur agar plates at 37 degrees c. no relationship between serotype and sensitivity was observed. the sensitivity of this species was strain-dependent and a large variation in tolerance to curvaticin 13 was observed. the combined effects of curvaticin ... | 2000 | 10849167 |
| physicochemical surface properties of five listeria monocytogenes strains from a pork-processing environment in relation to serotypes, genotypes and growth temperature. | physicochemical surface properties, related to electrostatic, van der waals and lewis acid-base interactions, of five listeria monocytogenes strains isolated from pork-processing environments were determined after two subcultures at 37 degrees c and a final culture at three temperatures: 37, 10 and 4 degrees c. three strains (lm1, lm114 and lm191) were genetically related while two were unrelated (lm25 and lm74) according to apai-macrorestriction and pulsed-field gel electrophoresis (pfge) typin ... | 2000 | 10849175 |
| solubility and antimicrobial efficacy of protamine on listeria monocytogenes and escherichia coli as influenced by ph. | the antimicrobial efficacy of protamine on listeria monocytogenes and escherichia coli was evaluated at concentrations from 50 to 10 000 microgram ml-1 and ph levels from 5.5 to 8.0. the minimum inhibitory concentrations decreased with increasing ph. protamine inhibited e. coli at all ph values while l. monocytogenes was inhibited at ph 6.5 and above. the antimicrobial efficacy of protamine decreased in the presence of negatively charged gelatine b but remained almost unchanged with addition of ... | 2000 | 10849181 |
| survey for psychrotrophic bacterial pathogens in minimally processed lettuce. | a total of 120 minimally processed, cut and packaged lettuce samples were purchased from retail supermarkets or provided by a salad production facility over an 8-month period. the samples were tested for total aerobic plate counts and for the presence of potentially pathogenic species belonging to the genera of listeria, aeromonas and yersinia. the aerobic plate counts ranged from 103 to 109 colony forming units (cfu) g-1. most samples (76%) contained between 105 and 107 cfu g-1 total aerobic ba ... | 2000 | 10849276 |
| a comparison of the bioscreen method and microscopy for the determination of lag times of individual cells of listeria monocytogenes. | lag phase durations (tlag) of individual listeria monocytogenes cells were analysed using the nightowl molecular imaging system, and results were compared with mean individual cell lag times (tl) obtained from the detection time (td) method using bioscreen. with bioscreen, an average tl of 6.39+/-0.89 h was obtained from five separate experiments. with the nightowl method, an average tlag of 2.73+/-0.06 h was obtained from three experiments consisting of eight total replicates. lag values from t ... | 2000 | 10849278 |
| pulsed-field gel electrophoresis for the analysis of listeria monocytogenes infection clusters after kidney transplantation. | listeria monocytogenes causes a rare, life-threatening infection in recipients of transplanted organs. we used cultures of blood and cerebrospinal fluid to characterize isolates and to distinguish cases in clusters from what might have been sporadic cases. from december 1994 to november 1995, six systemic l. monocytogenes infections occurred at our renal-transplantation unit. we confirmed the clinical diagnosis with blood and cerebrospinal fluid cultures and characterized the isolates retrospect ... | 2000 | 10850630 |
| treatment of listeriosis. | to review the most currently accepted treatment options for the treatment of listeriosis. | 2000 | 10852095 |
| survival of listeria innocua in salmon following cold-smoke application. | the ability of listeria innocua to survive on salmon fillets during cold smoking in a commercial processing plant was investigated using a central composite rotatable response surface design to examine smoking temperatures in the range of 18 to 30 degrees c and a smoke time from 2 to 14 h. smoke temperature did not significantly (p < 0.05) reduce counts of l. innocua on the salmon. however, the smoking time had a significant effect on l. innocua. the smoking time was directly related to the redu ... | 2000 | 10852563 |
| antibacterial mechanism of allyl isothiocyanate. | allyl isothiocyanate (aitc), a natural compound in plants belonging to the family cruciferae, has been shown to have strong antimicrobial activity in liquid media as well as in its vapor form. to understand its antimicrobial mechanism, aitc was tested for bactericidal activities to salmonella montevideo, escherichia coli o157:h7, and listeria monocytogenes scott a at different stages of growth and was compared with streptomycin, penicillin g, and polymyxin b, each of known antibacterial mechanis ... | 2000 | 10852565 |
| antibacterial activity of a chitooligosaccharide mixture prepared by cellulase digestion of shrimp chitosan and its application to milk preservation. | the antibacterial activity of a chitooligosaccharide mixture prepared by digestion of shrimp chitosan with cellulase at 50 degrees c for 14 h was evaluated. sugars with 1 to 8 degrees of polymer (dp) were found in this chitooligosaccharide mixture, and the weight percentage of sugars with dp > or = 6 was 44.3%. minimal lethal concentrations of this mixture against aeromonas hydrophila, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, shigella dysenteriae, ... | 2000 | 10852568 |
| a multidrug efflux transporter in listeria monocytogenes. | a chromosomal gene (mdrl) was found in listeria monocytogenes l028, showing a high degree of similarity with multidrug efflux transporters of the major facilitator superfamily (family 2). an allele-substituted mutant of this gene failed to pump out ethidium bromide and presented lower minimal inhibitory concentrations of macrolides, cefotaxime and heavy metals. this is the first multidrug efflux pump described in listeria. | 2000 | 10856655 |
| survival characteristics and the applicability of predictive mathematical modelling to listeria monocytogenes growth in sous vide products. | survival and growth of listeria monocytogenes isolates during sous vide processing and storage, and the applicability of predictive modelling in determining the potential for growth of l. monocytogenes in broth models and in sous vide products was investigated. l. monocytogenes grew in anaerobic tryptose phosphate broth and in chicken and beef samples by 2 log cycles in 8 days at 3 degrees c and 4-5 log cycles in 6 days at 8 degrees c. however, heating to an internal temperature of 70 degrees c ... | 2000 | 10857538 |
| survival of osmotic and acid stress by listeria monocytogenes strains of clinical or meat origin. | the ability of 30 listeria monocytogenes strains, 15 of meat origin and 15 of clinical origin, to use carnitine as an osmoprotectant and to resist acid stress was determined. all strains examined were able to use carnitine as an osmoprotectant, indicating the importance of this characteristic to the survival of l. monocytogenes in natural environments. clinical and meat strains, however, differed with respect to this characteristic. specifically, 73% of meat strains reached a lower maximum cell ... | 2000 | 10857542 |
| combined pcr and slot blot assay for detection of salmonella and listeria monocytogenes. | detection of salmonella typhimurium and listeria monocytogenes by the polymerase chain reaction (pcr) assay coupled with slot blot detection was investigated in this study. after being extracted from diluted bacterial culture with the extraction buffer, bacterial dna was subjected to pcr. the slot blot assay was optimized and used to detect pcr products. the lowest detection level of this method was 10(3) cfu/ml in the original culture media for both pathogens, or 5 bacterial cells in the pcr re ... | 2000 | 10857543 |
| a new rapid automated method for the detection of listeria from environmental swabs and sponges. | many food and meat processors test environmental swabs and sponges to confirm the absence of listeria spp. spectral pattern changes in a liquid growth medium, resulting from esculin hydrolysis by listeria in contaminated swabs and sponges, were automatically monitored by the biosys instrument in a semifluid layer (sfl). the blackening of sfl in modified mox broth resulted in sharply declining curves, which were easily detected by the instrument. the instrument detected all nine strains of lister ... | 2000 | 10857550 |
| mathematical modelling of the growth rate and lag time for listeria monocytogenes. | growth data for listeria monocytogenes were collected from the literature and a global model built with existing secondary models describing independently the effects of environmental factors on the growth rate and lag time was based on these data. the growth rates calculated with this model were consistent with the published ones but the fit was poor near the limits of growth of the micro-organism. the model was also less accurate to describe the lag time. it seems then that reliable prediction ... | 2000 | 10857924 |
| modelling the growth rate of listeria monocytogenes with a multiplicative type model including interactions between environmental factors. | a multiplicative secondary model previously published to describe independently the effects of environmental factors on the growth rate of listeria monocytogenes (augustin and carlier, 2000) was improved by taking into account interactions between these environmental factors. the proposed model allowed to decrease the rate of fail-safe growth predicted from 13.5% to 12.1% and the rate of fail-dangerous no growth predicted from 16.1% to 7.1%. | 2000 | 10857925 |
| host resistance to listeria monocytogenes infection is enhanced but resistance to staphylococcus aureus infection is reduced in acute graft-versus-host disease in mice. | acute graft-versus-host disease (gvhd) is characterized by the production of high levels of t helper 1 (th1)-type cytokines. bone marrow transplantation from allogeneic c57bl/6 cells to cbf(1) mice produced acute gvhd. host resistance to th1-driven listeria monocytogenes was enhanced, whereas host resistance to th2-driven staphylococcus aureus was reduced during acute gvhd. these results suggest that opposite host responses are observed between th1-driven and th2-driven bacterial infections in a ... | 2000 | 10858256 |
| downmodulation of the inflammatory response to bacterial infection by gammadelta t cells cytotoxic for activated macrophages. | although gammadelta t cells are involved in the regulation of inflammation after infection, their precise function is not known. intraperitoneal infection of t cell receptor (tcr)-delta(-/-) mice with the intracellular bacterium listeria monocytogenes resulted in the development of necrotic foci in the livers. in contrast, the peritoneal cavities of infected tcr-delta(-/-) mice contained an accumulation of low density activated macrophages and a reduced percentage of macrophages undergoing apopt ... | 2000 | 10859339 |
| detection of dna via an ion channel switch biosensor. | detection of dna by an ion channel switch biosensor has been demonstrated in a model system, using single-stranded oligonucleotide sequences of 52-84 bases in length. two different biotinylated probes are bound, via streptavidin, either to the outer region of a gramicidin ion channel dimer or to an immobilized membrane component. the ion channels are switched off upon detection of dna containing complementary epitopes to these probes, separated by a nonbinding region, at nanomolar levels. the dn ... | 2000 | 10860501 |
| cutting edge: antilisterial activity of cd8+ t cells derived from tnf-deficient and tnf/perforin double-deficient mice. | the mechanisms by which cd8+ t cells mediate immunity against bacterial pathogens remain largely unknown. perforin-dependent cytolysis plays a role, but is not required for cd8+ t cell-mediated immunity against listeria monocytogenes. tnf is essential for cd8+ t cell immunity to l. monocytogenes, but the cellular source of tnf is undefined. tnf-deficient and tnf/perforin double-deficient mice were used to generate cd8+ t cells specific for an l. monocytogenes-derived ag. wild-type and tnf-defici ... | 2000 | 10861027 |
| use of a ground beef model to assess the effect of the lactoperoxidase system on the growth of escherichia coli o157:h7, listeria monocytogenes and staphylococcus aureus in red meat. | the ability to preserve food in a state that is both appetising and nutritious is a basic requirement for health. food poisoning represents a major source of illness and loss of productivity in many developed countries. of particular concern in recent years are outbreaks of food poisoning associated with escherichia coli o157:h7 or listeria monocytogenes, many of which have been associated with the consumption of ground meat. many of the chemicals presently licensed for use as food preservatives ... | 2000 | 10868676 |
| a model describing the effect of temperature history on lag time for listeria monocytogenes. | a model was built to describe the influence of the temperature and the duration of pre-incubation on the lag time for regrowth of listeria monocytogenes at low temperature. this model is consistent with the usual procedure used to calculate lag times of cultures growing under fluctuating temperatures. it also describes the effect of prolonged starvation conditions on the regrowth lag time and takes into account the influence of the physiological state of inocula in predictive models. | 2000 | 10868678 |
| evaluation of a monoclonal antibody able to detect live listeria monocytogenes and listeria innocua. | a monoclonal listeria antibody, designated b4, was evaluated. the ability of the antibody to bind to viable bacteria belonging to listeria spp. compared to bacteria of the same species killed by heat treatment, acid or base treatment, sanitizers, and irradiation was examined. the antibody was found to react with viable l. monocytogenes and l. innocua, but not with heat-killed (72 degrees c, 5 min) strains of these organisms. when l. monocytogenes and l. innocua were killed by methods other than ... | 2000 | 10868683 |
| microbiologic features of adult community-acquired bacterial meningitis in taiwan. | community-acquired bacterial meningitis (cabm) is a life-threatening disease that requires prompt initiation of appropriate antibiotic therapy. the purpose of this study was to determine the causative microorganisms of cabm and their antimicrobial susceptibility patterns at a major teaching hospital in taipei from 1993 to 1998. | 2000 | 10870313 |
| characterization of iap gene in listeria monocytogenes strains isolated in japan. | variation of the iap gene region (407bp) encoding an invasion-associated protein p60 was studied on 12 strains of listeria monocytogenes of different origin in japan. these 12 strains are known to have 2 types of serotype (1/2a and 4b) and have a diversity among the strains (saito et al., 1998). the dye-primer cycle sequencing method was employed to determine the genomic structure, and the nucleotide sequences obtained were compared with those of reference strain sv 1/2a egd. differences found i ... | 2000 | 10872686 |
| differences in interleukin-12 and -15 production by dendritic cells at the early stage of listeria monocytogenes infection between balb/c and c57 bl/6 mice. | the mechanisms responsible for the resistance of c57bl/6 mice and for the susceptibility of balb/c mice to infection with listeria monocytogenes were studied by comparing early il-12 and il-15 production by dendritic cells (dc) after infection with l. monocytogenes. splenic dc expressing cd11b(low) and cd11c(+) obtained from c57bl/6 mice at 3 and 6 h after l. monocytogenes infection expressed higher levels of il-12 p40 mrna and il-12 p40 protein than did those from balb/c mice. concurrently, a l ... | 2000 | 10873304 |
| the presence of intragenically located rep-like elements in bacillus sporothermodurans is sufficient for rep-pcr typing. | the technique of repetitive extragenic palindromic polymerase chain reaction (rep-pcr) enables the identification and discrimination of clonally related bacillus sporothermodurans isolates from ultra-high temperature and sterilized milk. the aim of this study was to investigate the genetic basis for the generation of these highly informative rep-pcr patterns. the major 947-bp rep-pcr fragment of b. sporothermodurans was cloned, together with its 5' and 3' flanking sequences. only partial homolog ... | 2000 | 10875282 |
| listeria monocytogenes peritonitis: an unusual presentation and review of the literature. | listeria monotogenes bacteria-ascites developed in a 73-year-old man who had cholangiocarcinoma and liver metastasis. spontaneous bacterial peritonitis (sbp) is a frequent complication in patients with chronic liver disease and ascites. l. peritonitis has been reported in only <30 cases world-wide. our patient represents a unique case of l. peritonitis without peritoneal fluid analysis consistent with spontaneous bacteria peritonitis. however, the culture of the ascitic fluid provided the final ... | 2000 | 10875476 |
| [the interaction of yersinia, listeria and salmonella with plant cells]. | the results of the interaction of bacteria of the genera yersinia, listeria and salmonella, pathogenic for humans and animals, with callus cultures of different plant species are presented. as revealed in this study, complicated interactions developed between bacteria and plant cells. plant cells were shown to be highly sensitive to the action of bacteria. yersinia, listeria and salmonella were found to be capable of callus damage. the influence of plant cells on bacteria was more complicated: b ... | 2000 | 10876881 |
| [nutrient media for the isolation and accumulation of listeria]. | dried culture media for the isolation and accumulation of listeria from pathological material and foodstuffs have been developed. the media are suitable for use in bacteriological and sanitary-hygienic practice. the optimum nutrient base has been selected: dried broth (from sprat hydrolysate), produced by the research and manufacturing amalgamation "culture media". the optimum concentrations of ingredients, stimulating the growth of listeria and inhibiting the growth of associated microbes, have ... | 2000 | 10876892 |
| measurement of the elasticity of the actin tail of listeria monocytogenes. | we report biophysical experiments performed on the bacterium listeria monocytogenes, a model system to study actin-based motility. using optical tweezers and electrophoresis experiments, we find that the bacterium is firmly attached to its tail, and we demonstrate that the tail responds as an elastic gel when deformed. we have measured its elastic modulus at a value of 10(3)-10(4) pa, which is 10 times higher than the rigidity of the eukaryotic cytoplasm. these results demonstrate that the bacte ... | 2000 | 10877022 |
| gene cloning and expression and secretion of listeria monocytogenes bacteriophage-lytic enzymes in lactococcus lactis. | bacteriophage lysins (ply), or endolysins, are phage-encoded cell wall lytic enzymes which are synthesized late during virus multiplication and mediate the release of progeny virions. bacteriophages of the pathogen listeria monocytogenes encode endolysin enzymes which specifically hydrolyze the cross-linking peptide bridges in listeria peptidoglycan. ply118 is a 30.8-kda l-alanoyl-d-glutamate peptidase and ply511 (36.5 kda) acts as n-acetylmuramoyl-l-alanine amidase. in order to establish dairy ... | 2000 | 10877791 |
| absence of a putative mannose-specific phosphotransferase system enzyme iiab component in a leucocin a-resistant strain of listeria monocytogenes, as shown by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | leucocin a is a class iia bacteriocin produced by leuconostoc spp. that has previously been shown to inhibit the growth of listeria monocytogenes. a spontaneous resistant mutant of l. monocytogenes was isolated and found to be resistant to leucocin a at levels in excess of 2 mg/ml. the mutant showed no significant cross-resistance to nontype iia bacteriocins including nisaplin and esf1-7gr. however, there were no inhibition zones found on a lawn of the mutant when challenged with an extract cont ... | 2000 | 10877813 |
| virulent rough filaments of listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein. | atypical rough cell filaments of listeria monocytogenes (designated fr variants), isolated from clinical and food samples, form long filaments up to 96 microm in length and demonstrated wild-type levels of adherence, invasion, and cytotoxicity to human epithelial hep-2, caco-2, and hela cells. unlike previously described avirulent rough mutants of l. monocytogenes that secrete diminished levels of the major extracellular protein p60 and that form long chains that consist of multiple cells of sim ... | 2000 | 10878057 |
| a novel beta-glucoside-specific pts locus from streptococcus mutans that is not inhibited by glucose. | a regulon from streptococcus mutans that plays a role in the utilization of beta-glucosides has been isolated, sequenced and subjected to sequence analysis. this regulon encodes a beta-glucoside-specific enzyme ii (eii) component (bglp) of the phosphoenolpyruvate-dependent phosphotransferase system (pts) and a phospho-beta-glucosidase (bgla) which is responsible for the breakdown of the phospho-beta-glucosides within the cell. both the bglp and bgla gene products have significant similarity with ... | 2000 | 10878120 |
| the arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins. | the arp2/3 complex is an essential regulator of actin polymerization in response to signalling and generates a dendritic array of filaments in lamellipodia. here we show that the activated arp2/3 complex interacts with the barbed ends of filaments to initiate barbed-end branching. barbed-end branching by arp2/3 quantitatively accounts for polymerization kinetics and for the length correlation of the branches of filaments observed by electron microscopy. filament branching is visualized at the su ... | 2000 | 10878802 |
| exploiting the immune system: toward new vaccines against intracellular bacteria. | 2000 | 10879281 | |
| amplified fragment length polymorphism (aflp) analysis of listeria monocytogenes. | aflp analysis using four selective primers was performed on a set of 33 listeria monocytogenes including strains from patients and foods implicated in outbreaks, human sporadic cases or foods. strains were tested belonging to serovars 1/2a, 1/2b, 1/2c, 3b, and 4b. using one of the primers, the aflp technique generated 20 different sized dna fragments. the 33 cultures segregated into 14 different patterns, each comprising 7-12 different fragments. although the method was not sufficiently discrimi ... | 2000 | 10879987 |
| from hot dogs to cd8+ t cells: listeria monocytogenes. | 2000 | 10881333 | |
| a novel antibacterial agent derived from the c-terminal domain of streptococcus mutans gtp-binding protein. | a decapeptide, p1, homologous to part of the streptococcus mutans g-protein (sgp) and the escherichia coli era protein, was synthesized and examined for growth-inhibitory effects. when p1 10 mg/l was added to e. coli dh5, the viability of the cells was reduced by 13%. addition of lauric acid enhanced the bactericidal effects of p1 (68% killing in the presence of p1 plus lauric acid). similar enhancements were observed for mono lauroyl-rac-glycerol (mlg) and sodium dodecyl sulphate (sds). in cult ... | 2000 | 10882696 |
| [listeria monocytogenes incidence and evaluation of the sanitary quality of filleted fresh fish from the metropolitan area of san josé]. | fish constitutes a very important part of human diet due to its protein content. the poor commercialization system existing in costa rica for this product causes an important secondary contamination that may include microorganisms such as fecal coliforms, staphylococcus aureus and even pathogens such as listeria monocytogenes. there is a lack of recent information about the microbiological quality of the fish that arrives to the metropolitan area of san josé, costa rica, so the aim of this work ... | 1999 | 10883301 |
| growth condition-related response of listeria monocytogenes 412 to bacteriocin inactivation. | bacteriocin inactivation of listeria monocytogenes 412 was studied as a function of growth phase. cells were treated with nisin (300 iu ml-1) or pediocin (320 or 2560 au ml-1) for 20 min at 30 degrees c. inactivation with nisin or the low concentration of pediocin was growth phase dependent, with exponentially growing cells being more susceptible than stationary cells. no effect of growth phase was observed for the high pediocin concentration. pediocin inactivation (320 au ml-1) of l. monocytoge ... | 2000 | 10886618 |
| development of a direct viable count procedure for the investigation of vbnc state in listeria monocytogenes. | a viable but non-culturable (vbnc) bacterial state was originally detected in studies in environmental microbiology. in particular, this state has been demonstrated for a number of human pathogens (escherichia coli, salmonella enteritidis, vibrio cholerae, legionella pneumophila and campylobacter jejuni). the presence of vbnc cells poses a major public health problem since they cannot be detected by traditional culturing methods and the cells remain potentially pathogenic under favourable condit ... | 2000 | 10886620 |
| kombucha fermentation and its antimicrobial activity. | kombucha was prepared in a tea broth (0.5% w/v) supplemented with sucrose (10% w/v) by using a commercially available starter culture. the ph decreased steadily from 5 to 2.5 during the fermentation while the weight of the "tea fungus" and the od of the tea broth increased through 4 days of the fermentation and remained fairly constant thereafter. the counts of acetic acid-producing bacteria and yeasts in the broth increased up to 4 days of fermentation and decreased afterward. the antimicrobial ... | 2000 | 10888589 |
| rapid detection of low levels of listeria in foods and next-day confirmation of l. monocytogenes. | outbreaks of foodborne listeriosis caused by listeria monocytogenes in recent years, and the high mortality rate associated with listeriosis, have raised the need for reliable and rapid detection of the pathogen. a simple, automated method was developed for the detection of listeria organisms in foods. it consists of a 6-h pre-enrichment step followed by overnight incubation in selective broth at 35 degrees c. changes in light transmittance in the selective broth are registered continuously by a ... | 2000 | 10889308 |
| a colony lift immunoassay for the specific identification and quantification of listeria monocytogenes. | a colony lift immunoassay (cli) has been developed to detect listeria monocytogenes after the organisms have been cultured on filter membranes or agar plates. polyvinylidene fluoride membranes (pvdf) (millipore, bedford, ma), used in the cli, were prewet with methanol and used to imprint colonies that were grown on the filter or agar plates. a positive control was applied to the edge of each membrane. the imprinted membranes were subsequently air dried, peroxidase neutralized, blocked, and react ... | 2000 | 10889311 |
| roles of endogenous cytokines in liver apoptosis of mice in lethal listeria monocytogenes infection. | various bacterial pathogens have been identified as mediators of apoptosis. apoptosis reportedly shows both detrimental and beneficial effects on biological functions. we studied the role of liver apoptosis in lethal listeria monocytogenes infection and the regulation of apoptosis by endogenous cytokines during infection. apoptosis was observed in the spleen but not in the liver of infected mice, whereas the induction of liver necrosis was evident by rising levels of serum aminotransferases in t ... | 2000 | 10891658 |
| negative regulation of fibroblast motility by ena/vasp proteins. | ena/vasp proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. using overexpression, loss-of-function, and inhibitory approaches, we find that ena/vasp proteins negatively regulate fibroblast motility. a dose-dependent decrease in movement is observed when ena/vasp proteins are overexpressed in fibroblasts. neutralization or deletion of all ena/vasp proteins results in increased cell movement. selective ... | 2000 | 10892743 |
| listeria spp. in broiler flocks: recovery rates and species distribution investigated by conventional culture and the eiafoss method. | the occurrence of listeria monocytogenes in samples from broiler houses and cloacal swabs taken at the abattoir was investigated. an automated immunobased method (eiafoss) was used, and 42 samples were also analysed by conventional culture; both methods were based on a two-step selective enrichment using chr.4.17; fraser and fraser broths. l. monocytogenes was isolated from two of 71 broiler flocks, yielding an estimated flock prevalence of 3%. the flock prevalence of l. inocua was estimated to ... | 2000 | 10898468 |
| immunity to listeria infection. | infection with listeria monocytogenes is a well studied model for understanding host resistance to intracellular bacteria. recent advances in the study of listeria have carefully quantitated the response of cd8(+) t cells to infection and analyzed the effector functions of these cells in vivo. a surprising role for antibody in mediating resistance to listeria has also recently emerged, providing new insight into the mechanisms of host defense. | 2000 | 10899025 |
| adaptive immunity against listeria monocytogenes in the absence of type i tumor necrosis factor receptor p55. | tumor necrosis factor (tnf) and the type i tnf receptor (tnfri), p55, are critical for resistance against primary infections with the intracellular bacterial pathogen listeria monocytogenes. importantly, however, susceptibility to primary listeriosis in cytokine-deficient mice does not preclude the development or expression of effective adaptive immunity against virulent l. monocytogenes. we used tnfri(-/-) mice to study adaptive antilisterial immunity in the absence of interactions between tnf ... | 2000 | 10899844 |
| interleukin-10 has different effects on proliferation of listeria monocytogenes in livers and spleens of mice. | the aim of this study was to investigate the effect of interleukin-10 (il-10) on the course of listeria monocytogenes infection in naive and immune mice. treatment with il-10 during the course of a primary infection significantly decreased the number of bacteria in the spleen and did not affect the number in the liver. during a secondary infection in immune mice treated with il-10, the number of bacteria was significantly lower in the spleen but significantly higher in the liver in comparison to ... | 2000 | 10899871 |
| ultrastructure of rickettsia rickettsii actin tails and localization of cytoskeletal proteins. | actin-based motility (abm) is a mechanism for intercellular spread that is utilized by vaccinia virus and the invasive bacteria within the genera rickettsia, listeria, and shigella. within the rickettsia, abm is confined to members of the spotted fever group (sfg), such as rickettsia rickettsii, the agent of rocky mountain spotted fever. infection by each agent induces the polymerization of host cell actin to form the typical f (filamentous)-actin comet tail. assembly of the actin tail propels t ... | 2000 | 10899876 |
| critical role of neutrophils in eliminating listeria monocytogenes from the central nervous system during experimental murine listeriosis. | neutrophils are the main inflammatory cell present in lesions involving the central nervous system (cns) during human and murine listeriosis. in this study, administration of the neutrophil-depleting monoclonal antibody rb6-8c5 during experimental murine listeriosis facilitated the multiplication of listeria monocytogenes in the cns. these data suggest that neutrophils play a key role in eliminating bacteria that gain access to the cns compartment. in addition, we provide evidence that their mig ... | 2000 | 10899890 |
| humoral and cell-mediated immunity in mice with genetic deficiencies of prolactin, growth hormone, insulin-like growth factor-i, and thyroid hormone. | prolactin (prl), growth hormone (gh), insulin-like growth factor-i (igf-i), and thyroid hormones have been proposed as critical immunoregulatory mediators, and their clinical use is being considered. the precise role played by each of these hormones in the generation of humoral and cell-mediated immune responses was assessed in a panel of mice with mutations that result in a selective reduction of prl, gh, igf-i, and/or thyroid hormone production. a surprising result, in view of previous studies ... | 2000 | 10900161 |
| listeriosis in a raccoon dog (nyctereutes procyonoides) associated with canine distemper. | a wild raccoon dog (nyctereutes procyonoides) that manifested severe illness and died was examined. necropsy revealed severe emaciation, systemic icterus and petechial hemorrhages on the mucous membranes. histopathologically, necroses were seen in the liver and brain stem associated with meningitis. eosinophilic intranuclear inclusion bodies were observed in the spleen and intestinal mucosa, and eosinophilic intracytoplasmic inclusion bodies were seen in transitional epithelium in the bladder. l ... | 2000 | 10907692 |
| incidence of listeria monocytogenes in wild animals in japan. | between 1991 and 1993, the intestinal contents and feces of wild animals in japan were examined for the presence of listeria. the wild animals examined included 623 mammals (11 species) and 996 birds (18 species). listeria species were isolated from 38 (6.1%) of the 623 mammalian samples and 133 (13.4%) of 996 bird samples. the highest incidence of listeria in the mammals was found in japanese monkeys (20.0%) and that in birds was found in crows (43.2%). the incidence of listeria in japanese mon ... | 2000 | 10907701 |
| rapid membrane capture pcr-based method for detection of listeria species. | 1998 | 10909835 | |
| listeria monocytogenes as an alternative vaccine vector for hiv. | the necessity for an hiv vaccine and a brief review of current strategies towards this aim are given here to set into context contemporary studies towards exploiting the bacterium listeria monocytogenes as an hiv vaccine vector. the cell biology and immunology of this unusual intracellular organism are also reviewed, in addition to its application to introducing viral antigens, including hiv antigens, to the immune system. | 2000 | 10912619 |
| current status and future perspectives of dna vaccine delivery by attenuated intracellular bacteria. | vaccination by intradermal or intramuscular injection of antigen-encoding plasmid-dna elicits strong cellular and humoral immune responses. professional antigen presenting cells (apc) seem to induce these responses, making it, therefore, desirable to deliver the plasmid molecules directly to these cells. the exploitation of attenuated intracellular bacteria as dna delivery vehicles makes the direct targeting of dna vaccine vectors to professional apc feasible. | 2000 | 10912622 |
| antimicrobial activity of a 14-residue synthetic peptide against foodborne microorganisms. | a chemically synthesized short-chain peptide composed of six leucine and eight lysine (6k8l) residues was demonstrated to be biocidal against several foodborne organisms including escherichia coli o157:h7, listeria monocytogenes, pseudomonas fluorescens, and kluyveromyces marxianus suspended in phosphate buffer at concentrations of 5 to 50 microg/ml. all strains were reduced by 3 log10 cfu/ml within 10 min at peptide concentrations of <10 microg/ml. the peptide reduced by 3 log10 cfu/ml e. coli ... | 2000 | 10914655 |
| effectiveness of two cooking systems in destroying escherichia coli o157:h7 and listeria monocytogenes in ground beef patties. | a rapid, high-temperature double-sided grilling-broiling (dgb) system was compared to a single-sided broiling (ssb) system for cooking of foodservice ground beef patties to reduce microbial numbers and maintain textural quality. patties (110 g) containing either escherichia coli o157:h7 or listeria monocytogenes (10(6-7) cfu/g) were cooked to target internal temperatures of 60 or 68 degrees c on each cooking system and immediately removed from the grills without the additional holding time at 60 ... | 2000 | 10914656 |
| growth and survival of escherichia coli o157:h7 and listeria monocytogenes in egg products held at different temperatures. | growth and survival of escherichia coli o157:h7 and listeria monocytogenes in steamed eggs and scrambled eggs held at different temperatures (5, 18, 22, 37, 55, and 60 degrees c) were investigated in the present study. among the holding temperatures tested, both pathogens multiplied best at 37 degrees c followed by 22, 18, and 5 degrees c. in general, e. coli o157:h7 grew better in the egg products than l. monocytogenes did at all the storage temperatures tested except at 5 degrees c. e. coli o1 ... | 2000 | 10914658 |
| behavior of listeria monocytogenes in pasteurized milk during fermentation with lactic acid bacteria. | the behavior of listeria monocytogenes in pasteurized milk during fermentation with starter and nonstarter lactic acid bacteria was investigated. pasteurized milk was co-inoculated with approximately 10(4) cfu/ml of l. monocytogenes and 10(6) cfu/ml of lactococcus lactis, lactococcus cremoris, lactobacillus plantarum, lactobacillus bulgaricus, or streptococcus thermophilus. inoculated milks were incubated at 30 degrees c or 37 degrees c for 24 to 72 h. listeria monocytogenes survived and also gr ... | 2000 | 10914660 |
| use of capillary tubes and plate heat exchanger to validate u.s. department of agriculture pasteurization protocols for elimination of listeria monocytogenes in liquid egg products. | d-values for a five-strain cocktail of listeria monocytogenes in five different liquid egg products (whole egg, egg yolk, egg white, egg yolk + 5% sucrose + 5% nacl, and egg yolk + 10% nacl) were determined using 100-microl capillary tubes. the egg products were inoculated with approximately 1 x 10(10) organisms/ml and heated in capillary tubes to temperatures ranging from 53 to 69 degrees c for various time intervals. using a pilot scale plate heat exchanger, the u.s. department of agriculture ... | 2000 | 10914661 |
| natural variation in susceptibility of listeria strains to class iia bacteriocins. | thirty-one listeria strains were tested for sensitivity to four class iia bacteriocins, namely, enterocin a, mesentericin y105, divercin v41, and pediocin ach, and to nisin a. class iia bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, whereas nisin a showed a different pattern in which all listeria strains were inhibited. particularly, it was observed that the strain listeria monocytogenes v7 could not be inhibited by ... | 2000 | 10919390 |
| optimization of green fluorescent protein expression vectors for in vitro and in vivo detection of listeria monocytogenes. | the green fluorescent protein (gfp) of the jellyfish aequorea victoria is a useful reporter molecule for monitoring in vivo gene expression in eukaryotic and prokaryotic cells. we constructed a series of gfp vectors for in situ detection of the intracellular pathogen listeria monocytogenes. the gfp-mutl gene, which encodes a red-shifted gfp, was transcriptionally fused to a strong l. monocytogenes promoter and inserted into various escherichia coli-listeria shuttle vectors: i) the integrative mo ... | 2000 | 10919515 |
| real-time measurements of the interaction between single cells of listeria monocytogenes and nisin on a solid surface. | a method to obtain real-time measurements of the interactions between nisin and single cells of listeria monocytogenes on a solid surface was developed. this method was based on fluorescence ratio-imaging microscopy and measurements of changes in the intracellular ph (ph(i)) of carboxyfluorescein succinimidyl ester-stained cells during exposure to nisin. immobilized cells were placed in a chamber mounted on a microscope and attached to a high-precision peristaltic pump which allowed rapid change ... | 2000 | 10919824 |
| a framework for interpreting the leucine-rich repeats of the listeria internalins. | the surface protein inlb of the bacterial pathogen listeria monocytogenes is required for inducing phagocytosis in various nonphagocytic mammalian cell types in vitro. inlb causes tyrosine phosphorylation of host cell adaptor proteins, activation of phosphoinositide 3-kinase, and rearrangements of the actin cytoskeleton. these events lead to phagocytic uptake of the bacterium by the host cell. inlb belongs to the internalin family of listeria proteins, which also includes inla, another surface p ... | 2000 | 10922035 |
| rapid molecular typing of listeria monocytogenes by pulsed-field gel electrophoresis. | pulsed-field gel electrophoresis (pfge) is a highly discriminating tool for molecular typing, but the conventional pfge protocol is time consuming. this paper describes a rapid method of pfge for listeria monocytogenes that yields results within 2 days. | 2000 | 10924402 |
| three regions within acta promote arp2/3 complex-mediated actin nucleation and listeria monocytogenes motility. | the listeria monocytogenes acta protein induces actin-based motility by enhancing the actin nucleating activity of the host arp2/3 complex. using systematic truncation analysis, we identified a 136-residue nh(2)-terminal fragment that was fully active in stimulating nucleation in vitro. further deletion analysis demonstrated that this fragment contains three regions, which are important for nucleation and share functional and/or limited sequence similarity with host wasp family proteins: an acid ... | 2000 | 10931865 |
| a simple procedure for determining the presence and concentration of listeria monocytogenes in dairy products. | this paper presents a simple method for determining both the presence and concentration of listeria monocytogenes in dairy products. the method involves application of the most probable number (mpn) technique and enrichment of a 25 g sample. our tests showed that the mpn correlates with the colony forming units (cfu), and estimated concentrations of as low as 1 bacterium/gr or less. we also studied the influence of listeria innocua as an accompanying flora. we detected l. monocytogenes, even in ... | 1997 | 10932725 |
| modulation of acta gene expression in listeria monocytogenes by iron. | this study analysed the invasiveness of listeria monocytogenes into enterocyte-like caco-2 cells in which iron depletion was achieved by picolinic acid treatment. both entry and intracellular multiplication varied depending on the endogenous iron content of bacterial and eukaryotic cells. the behaviour within enterocytes was correlated with a 10-fold increased transcription of the acta gene observed in bacterial cells grown under conditions of iron stress. | 2000 | 10933250 |
| in vivo cytokine production and resistance to infection after acute exposure to 3,4-dichloropropionaniline. | 3,4-dichloropropionaniline (propanil) is an extensively used postemergent herbicide that has been shown to produce toxic and immunotoxic effects. the present report examined if acute exposure to propanil altered in vitro or in vivo cytokine production in response to antigenic stimulation. studies to determine resistance to infection by the intracellular bacterium listeria monocytogenes after exposure to propanil were also conducted. our experiments demonstrate that in vivo exposure to propanil d ... | 2000 | 10933756 |
| in vivo and in vitro assessment of the virulence of listeria monocytogenes strains. | to evaluate whether the in vitro model (invasion and intracellular growth in caco-2 cells) for determining virulence is a suitable alternative to the in vivo model (50% lethal dose), we compared the levels of virulence obtained with the two models. we tested l. monocytogenes strains isolated from food and clinical samples during three episodes of listeriosis occurring in italy in the period 1993-1995. we also tested l. monocytogenes strains isolated from food during official control activities. ... | 2000 | 10939044 |
| quantitative risk assessment for listeria monocytogenes in smoked or gravad salmon and rainbow trout in sweden. | the objective of the present work was to develop a quantitative risk assessment model in which the exposure and risk of acquiring listeriosis from consumption of packaged smoked or gravad salmon and rainbow trout were estimated. an excel spreadsheet model was constructed in which variables were represented by distributions based on surveys of l. monocytogenes in these food products, and on demographic and consumption data. growth or inactivation was not included in the model. the model was run t ... | 2000 | 10939268 |
| comparison of microbiological risk assessment studies published. | the fao/who risk analysis framework and principles are in the process of being implemented in different national and international settings. the importance of a further development of international co-operation based upon agreed principles in this area is stressed in the codex alimentarius system, as well as under the wto/sps agreements. key input in this context will come from the presentation of formalised microbiological risk assessments. the number of peer-review published microbiological ri ... | 2000 | 10939269 |
| mycobacterium tuberculosis h37rv comparative gene-expression analysis in synthetic medium and human macrophage. | mycobacteria are intracellular pathogens that survive and grow in host macrophages. following phagocytosis, sustained intracellular bacterial growth depends on its ability to avoid destruction by macrophage-mediated host defences such as lysosomal enzymes, reactive oxygen and the reactive nitrogen intermediates. this suggests that the interaction between host cell and microbe is delicately balanced, and can be tipped in favour of either organism. the identification of mycobacterium tuberculosis ... | 2000 | 10940566 |
| dithiothreitol enhances listeria monocytogenes mediated cell cytotoxicity. | the hybridoma ped-2e9 based cytotoxicity assay was developed to distinguish virulent from avirulent listeria species in 6 hr. the cytotoxicity effect on ped-2e9 was reported to be primarily due the cytolytic action of listeriolysin o (llo), produced by l. monocytogenes. in this study, the effect of a reducing agent, dithiothreitol (dtt, 0-2 mm) that is known to activate llo was investigated to make the ped-2e9 based cytotoxicity assay an even more sensitive and rapid. also, we examined the effec ... | 2000 | 10941925 |
| the sophisticated survival strategies of the pathogen listeria monocytogenes. | the function of the acta protein of listeria monocytogenes has been partially elucidated. these results illustrate the sophistication with which intracellular pathogens like listeria use the host cell to their advantage, and have provided new insights into some of the molecular mechanisms of complex cell functions such as actin-promoted cell motility. the clarification of these processes is of fundamental importance not only for understanding elementary processes such as development and growth, ... | 1998 | 10943336 |
| consumption of eicosapentaenoic acid and docosahexaenoic acid impair murine interleukin-12 and interferon-gamma production in vivo. | in mice, individual dietary omega-3 polyunsaturated fatty acids n-3 (pufa) were found to be sufficient to effect the changes in circulating interleukin (il)-12 and interferon (ifn)-gamma levels that were previously seen in fish oil-fed mice. weanling female c3h mice were fed one of five experimental diets. all five diets met all known nutritional requirements for mice and differed only in the fat source. after 4 weeks, mice were challenged with live listeria monocytogenes or sterile pbs. twenty- ... | 2000 | 10944484 |
| immunostimulatory dna sequences help to eradicate intracellular pathogens. | 2000 | 10944809 | |
| activation of the innate immune system by cpg oligodeoxynucleotides: immunoprotective activity and safety. | 2000 | 10944812 | |
| rapid detection of salmonella typhimurium in chicken carcass wash water using an immunoelectrochemical method. | an immunoelectrochemical method coupled with immunomagnetic separation was developed for rapid detection of salmonella typhimurium in chicken carcass wash water. samples of chicken carcass wash water were inoculated with salmonella typhimurium at different cell numbers. possible nonspecified inhibitors in the wash water were minimized by filtration and centrifugation. an approximately 9.4% loss of salmonella cells was found after filtration (p < 0.01). the samples were mixed with anti-salmonella ... | 2000 | 10945578 |
| comparison of different reducing agents for enhanced detection of heat-injured listeria monocytogenes. | the effect of different reducing agents (l-cysteine, oxyrase, and enterococcus faecium) and their combinations on the detection of heat-injured (62.8 degrees c, 7.5 min or 10 min) listeria monocytogenes was examined. the incorporation of l-cysteine (0.5 g/liter) yielded higher percentage detection than any of the other reducing agents (p < 0.05). the combination of oxyrase (10 u/ml) and e. faecium (10(3) cfu/ml) synergistically enhanced the detection of l. monocytogenes heat-injured for 10 min a ... | 2000 | 10945581 |
| feasibility of a defined microflora challenge method for evaluating the efficacy of foodborne listeria monocytogenes selective enrichments. | comparison of isolation methods for microbial pathogens is complicated by the variable interference caused by the competitive microflora present in test samples such as foods. in principle, using measured amounts of a standard competitor in a defined surrogate food matrix might control the effect of variable interference. this possibility was investigated using listeria monocytogenes and enrichment broths belonging to the acriflavine-nalidixate selective agent class. triplicate test sample sets ... | 2000 | 10945582 |
| high-pressure processing applied to cooked sausages: bacterial populations during chilled storage. | vacuum-packaged cooked sausages were pressurized at 500 mpa for 5 or 15 min at mild temperature (65 degrees c) and later stored at 2 and 8 degrees c for 18 weeks. counts of aerobic mesophiles and psychrotrophs, lactic acid bacteria, enterobacteria, baird-parker microbiota, and listeria spp. were determined 1 day and 3, 6, 9, 12, 15, and 18 weeks after treatment and compared with those of cooked sausages treated at 80 to 85 degrees c for 40 min. pressurization generated reductions of about 4 log ... | 2000 | 10945586 |
| recalls of foods and cosmetics due to microbial contamination reported to the u.s. food and drug administration. | in the u.s., food product recalls serve as an important intervention in stemming the consumption of food products contaminated with infectious disease agents. we summarize the number and nature of foods and cosmetics recalled as a result of microbial contamination reported to the u.s. food and drug administration (fda) for the period 1 october 1993 through 30 september 1998. during this period, microbial contamination of food and cosmetic products was the leading cause for recalls, accounting fo ... | 2000 | 10945589 |
| microbiological safety of mayonnaise, salad dressings, and sauces produced in the united states: a review. | the literature on the death and survival of foodborne pathogens in commercial mayonnaise, dressing, and sauces was reviewed and statistically analyzed with emphasis on salmonella, escherichia coli o157:h7, and listeria monocytogenes. the absence of reports of foodborne illness associated directly with the consumption of commercially prepared acidic dressings and sauces is evidence of their safety. salmonella, e. coli o157:h7, e. coli, l. monocytogenes, staphylococcus aureus, and yersinia enteroc ... | 2000 | 10945595 |
| cytokine profile and natural killer cell activity in listeria monocytogenes infected mice treated orally with petiveria alliacea extract. | in this work, we investigated the effects of petiveria alliacea extract on the production of th1-type and th2-type cytokines and on nk cells activity in normal and listeria monocytogenes infected mice. our results demonstrated that in normal/non-infected mice p. alliacea administration led to increased levels of interleukin-2 (il-2). the infection alone enhanced inf-gamma levels and nk cell activity at 48 and 72 hours of infection. the treatment with five consecutive doses of 1000 mg/kg/day of p ... | 2000 | 10946828 |
| evaluation of the impact of short-term temperature abuse on the microbiology and shelf life of a model ready-to-use vegetable combination product. | the growth dynamics of indigenous aerobic mesophilic populations (amp), lactic acid bacteria (lab) and inoculated (listeria spp.) microbial populations on cooked and fresh vegetable products, packaged as separate entities and in combination, subjected to temperature fluctuation, were assessed. microbial proliferation was temperature and product dependent, being most pronounced at 12 degrees c in all products with maximum growth rates of 0.140, 0.175 and 0.126 log10 cfu/g per h being identified f ... | 2000 | 10946838 |
| prevalence and contamination levels of listeria monocytogenes in retail foods in japan. | retail foods in japan were surveyed for the presence and contamination levels of l. monocytogenes. it was isolated from 12.2, 20.6, 37.0 and 25.0% of 41 minced beef, 34 minced pork, 46 minced chicken and 16 minced pork-beef mixture samples, respectively. mpn values were higher than 100/g in five (10.9%) minced chicken samples, but lower than 100/g in all minced beef, pork and pork-beef mixture samples. the organism was also isolated from 5.4% of the 92 smoked salmon samples at mpn values lower t ... | 2000 | 10946841 |
| induction of interleukin-4 (il-4) by legionella pneumophila infection in balb/c mice and regulation of tumor necrosis factor alpha, il-6, and il-1beta. | infection of balb/c mice with a sublethal concentration of legionella pneumophila causes an acute disease that is resolved by innate immune responses. the infection also initiates the development of adaptive th1 responses that protect the mice from challenge infections. to study the early responses, cytokines induced during the first 24 h after infection were examined. in the serum, interleukin-12 (il-12) was detectable by 3 h and peaked at 10 h, while gamma interferon was discernible by 5 h and ... | 2000 | 10948149 |
| interleukin-1 signaling is essential for host defense during murine pulmonary tuberculosis. | interleukin (il)-1 signaling is required for the containment of infections with intracellular microorganisms, such as listeria monocytogenes and leishmania major. to determine the role of il-1 in the host response to tuberculosis, we infected il-1 type i receptor-deficient (il-1r(-/-)) mice, in which il-1 does not exert effects, with mycobacterium tuberculosis. il-1r(-/-) mice were more susceptible to pulmonary tuberculosis, as reflected by an increased mortality and an enhanced mycobacterial ou ... | 2000 | 10950787 |
| antibodies to the junctional adhesion molecule cause disruption of endothelial cells and do not prevent leukocyte influx into the meninges after viral or bacterial infection. | a hallmark of infectious meningitis is the invasion of leukocytes into the subarachnoid space. in experimental meningitis triggered by tumor necrosis factor-alpha and interleukin-1beta, the interaction of leukocytes with endothelial cells and the subsequent migration of the cells through the vessel wall can be inhibited by an antibody to the junctional adhesion molecule (jam). in contrast to the cytokine-induced meningitis model, anti-jam antibodies failed to prevent leukocyte influx into the ce ... | 2000 | 10950802 |
| an updated model of cell-mediated immunity--listeriosis: clinical and research aspects. | listeria monocytogenes causes sepsis and meningitis in immunocompromised hosts and a devastating maternal/fetal infection in pregnant women. in recent years a more benign gastroenteritis in normal hosts has been described. listeria has been increasingly identified as a food-borne pathogen, and large-scale contamination of processed foods with resulting outbreaks has occurred in recent years, possibly as a result of consolidation of the food industry. experimental listeriosis in mice has proven t ... | 2000 | 10951886 |
| mutations of arginine residues within the 146-kkrrk-150 motif of the acta protein of listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the arp2/3 complex. | the recruitment of actin to the surface of intracellular listeria monocytogenes and subsequent tail formation is dependent on the expression of the bacterial surface protein acta. of the different functional domains of acta identified thus far, the n-terminal region is absolutely required for actin filament recruitment and intracellular motility. mutational analysis of this domain which abolished actin recruitment by intracellular listeria monocytogenes identified two arginine residues within th ... | 2000 | 10954425 |